KR20240039167A - 인덱스 스플린트 올리고를 사용하는 결찰에 의한 긴 핵산의 조립 - Google Patents

인덱스 스플린트 올리고를 사용하는 결찰에 의한 긴 핵산의 조립 Download PDF

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Publication number
KR20240039167A
KR20240039167A KR1020247006522A KR20247006522A KR20240039167A KR 20240039167 A KR20240039167 A KR 20240039167A KR 1020247006522 A KR1020247006522 A KR 1020247006522A KR 20247006522 A KR20247006522 A KR 20247006522A KR 20240039167 A KR20240039167 A KR 20240039167A
Authority
KR
South Korea
Prior art keywords
sequence
index
splint
oligonucleotide
stranded oligonucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
KR1020247006522A
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English (en)
Korean (ko)
Inventor
데이비드 타우식
니콜라스 엠 삼파스
Original Assignee
애질런트 테크놀로지스, 인크.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 애질런트 테크놀로지스, 인크. filed Critical 애질런트 테크놀로지스, 인크.
Publication of KR20240039167A publication Critical patent/KR20240039167A/ko
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020247006522A 2021-08-02 2022-08-02 인덱스 스플린트 올리고를 사용하는 결찰에 의한 긴 핵산의 조립 Pending KR20240039167A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163228448P 2021-08-02 2021-08-02
US63/228,448 2021-08-02
PCT/US2022/039193 WO2023014728A1 (en) 2021-08-02 2022-08-02 Assembly of long nucleic acids by ligation using indexed splint oligos

Publications (1)

Publication Number Publication Date
KR20240039167A true KR20240039167A (ko) 2024-03-26

Family

ID=85153280

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020247006522A Pending KR20240039167A (ko) 2021-08-02 2022-08-02 인덱스 스플린트 올리고를 사용하는 결찰에 의한 긴 핵산의 조립

Country Status (6)

Country Link
US (2) US12281302B2 (https=)
EP (1) EP4381063A4 (https=)
JP (1) JP2024528094A (https=)
KR (1) KR20240039167A (https=)
CN (1) CN117716030A (https=)
WO (1) WO2023014728A1 (https=)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6495318B2 (en) 1996-06-17 2002-12-17 Vectorobjects, Llc Method and kits for preparing multicomponent nucleic acid constructs
WO2006127423A2 (en) * 2005-05-18 2006-11-30 Codon Devices, Inc. Methods of producing polynucleotide libraries using scarless ligation
US10538796B2 (en) 2016-10-13 2020-01-21 Agilent Technologies, Inc. On-array ligation assembly
US20230008217A1 (en) 2019-12-17 2023-01-12 Agilent Technologies, Inc. Ligation-based gene editing using crispr nickase

Also Published As

Publication number Publication date
EP4381063A4 (en) 2025-07-02
EP4381063A1 (en) 2024-06-12
US20230037679A1 (en) 2023-02-09
US12281302B2 (en) 2025-04-22
US20250250561A1 (en) 2025-08-07
JP2024528094A (ja) 2024-07-26
CN117716030A (zh) 2024-03-15
WO2023014728A1 (en) 2023-02-09

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Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20240227

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20250703

Comment text: Request for Examination of Application