JP2024528094A - インデックスを付したスプリントオリゴを使用したライゲーションによる長い核酸のアセンブリー - Google Patents
インデックスを付したスプリントオリゴを使用したライゲーションによる長い核酸のアセンブリー Download PDFInfo
- Publication number
- JP2024528094A JP2024528094A JP2024505406A JP2024505406A JP2024528094A JP 2024528094 A JP2024528094 A JP 2024528094A JP 2024505406 A JP2024505406 A JP 2024505406A JP 2024505406 A JP2024505406 A JP 2024505406A JP 2024528094 A JP2024528094 A JP 2024528094A
- Authority
- JP
- Japan
- Prior art keywords
- sequence
- splint
- index
- indexed
- oligonucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/501—Ligase
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163228448P | 2021-08-02 | 2021-08-02 | |
| US63/228,448 | 2021-08-02 | ||
| PCT/US2022/039193 WO2023014728A1 (en) | 2021-08-02 | 2022-08-02 | Assembly of long nucleic acids by ligation using indexed splint oligos |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2024528094A true JP2024528094A (ja) | 2024-07-26 |
| JP2024528094A5 JP2024528094A5 (https=) | 2025-07-31 |
Family
ID=85153280
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2024505406A Pending JP2024528094A (ja) | 2021-08-02 | 2022-08-02 | インデックスを付したスプリントオリゴを使用したライゲーションによる長い核酸のアセンブリー |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US12281302B2 (https=) |
| EP (1) | EP4381063A4 (https=) |
| JP (1) | JP2024528094A (https=) |
| KR (1) | KR20240039167A (https=) |
| CN (1) | CN117716030A (https=) |
| WO (1) | WO2023014728A1 (https=) |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6495318B2 (en) | 1996-06-17 | 2002-12-17 | Vectorobjects, Llc | Method and kits for preparing multicomponent nucleic acid constructs |
| WO2006127423A2 (en) * | 2005-05-18 | 2006-11-30 | Codon Devices, Inc. | Methods of producing polynucleotide libraries using scarless ligation |
| US10538796B2 (en) | 2016-10-13 | 2020-01-21 | Agilent Technologies, Inc. | On-array ligation assembly |
| US20230008217A1 (en) | 2019-12-17 | 2023-01-12 | Agilent Technologies, Inc. | Ligation-based gene editing using crispr nickase |
-
2022
- 2022-08-02 US US17/879,530 patent/US12281302B2/en active Active
- 2022-08-02 JP JP2024505406A patent/JP2024528094A/ja active Pending
- 2022-08-02 WO PCT/US2022/039193 patent/WO2023014728A1/en not_active Ceased
- 2022-08-02 KR KR1020247006522A patent/KR20240039167A/ko active Pending
- 2022-08-02 CN CN202280053115.XA patent/CN117716030A/zh active Pending
- 2022-08-02 EP EP22853815.3A patent/EP4381063A4/en active Pending
-
2025
- 2025-03-21 US US19/086,557 patent/US20250250561A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP4381063A4 (en) | 2025-07-02 |
| EP4381063A1 (en) | 2024-06-12 |
| US20230037679A1 (en) | 2023-02-09 |
| US12281302B2 (en) | 2025-04-22 |
| US20250250561A1 (en) | 2025-08-07 |
| KR20240039167A (ko) | 2024-03-26 |
| CN117716030A (zh) | 2024-03-15 |
| WO2023014728A1 (en) | 2023-02-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20250723 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20250723 |