KR20240033606A - Method for producing low-molecular-weight collagen using tongue sole skin - Google Patents
Method for producing low-molecular-weight collagen using tongue sole skin Download PDFInfo
- Publication number
- KR20240033606A KR20240033606A KR1020220116606A KR20220116606A KR20240033606A KR 20240033606 A KR20240033606 A KR 20240033606A KR 1020220116606 A KR1020220116606 A KR 1020220116606A KR 20220116606 A KR20220116606 A KR 20220116606A KR 20240033606 A KR20240033606 A KR 20240033606A
- Authority
- KR
- South Korea
- Prior art keywords
- collagen
- molecular
- low
- skin
- weight
- Prior art date
Links
- 102000008186 Collagen Human genes 0.000 title claims abstract description 173
- 108010035532 Collagen Proteins 0.000 title claims abstract description 173
- 229920001436 collagen Polymers 0.000 title claims abstract description 173
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 32
- 241001595453 Symphurus nigrescens Species 0.000 title 1
- 240000001980 Cucurbita pepo Species 0.000 claims abstract description 39
- 235000000832 Ayote Nutrition 0.000 claims abstract description 37
- 235000009854 Cucurbita moschata Nutrition 0.000 claims abstract description 37
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 claims abstract description 37
- 235000015136 pumpkin Nutrition 0.000 claims abstract description 37
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 34
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 29
- 239000006228 supernatant Substances 0.000 claims abstract description 28
- 102000004190 Enzymes Human genes 0.000 claims abstract description 21
- 108090000790 Enzymes Proteins 0.000 claims abstract description 21
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims abstract description 17
- 229920002674 hyaluronan Polymers 0.000 claims abstract description 17
- 229960003160 hyaluronic acid Drugs 0.000 claims abstract description 17
- 238000002156 mixing Methods 0.000 claims abstract description 17
- 239000011780 sodium chloride Substances 0.000 claims abstract description 17
- 239000000243 solution Substances 0.000 claims abstract description 17
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000012535 impurity Substances 0.000 claims abstract description 14
- 239000007787 solid Substances 0.000 claims abstract description 14
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims abstract description 12
- 235000017491 Bambusa tulda Nutrition 0.000 claims abstract description 12
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims abstract description 12
- 239000007864 aqueous solution Substances 0.000 claims abstract description 12
- 239000011425 bamboo Substances 0.000 claims abstract description 12
- 238000001914 filtration Methods 0.000 claims abstract description 12
- 238000003756 stirring Methods 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 9
- 239000008280 blood Substances 0.000 claims abstract description 8
- 210000004369 blood Anatomy 0.000 claims abstract description 8
- 239000003814 drug Substances 0.000 claims abstract description 7
- 238000010438 heat treatment Methods 0.000 claims abstract description 5
- 230000001954 sterilising effect Effects 0.000 claims abstract description 5
- 238000005406 washing Methods 0.000 claims abstract description 5
- 210000003097 mucus Anatomy 0.000 claims abstract description 4
- 230000001133 acceleration Effects 0.000 claims description 4
- 235000008216 herbs Nutrition 0.000 claims description 4
- 239000004615 ingredient Substances 0.000 claims description 4
- 244000061520 Angelica archangelica Species 0.000 claims description 3
- 241000218157 Aquilegia vulgaris Species 0.000 claims description 3
- 241001061264 Astragalus Species 0.000 claims description 3
- 102000018389 Exopeptidases Human genes 0.000 claims description 3
- 108010091443 Exopeptidases Proteins 0.000 claims description 3
- 241000287828 Gallus gallus Species 0.000 claims description 3
- 235000001287 Guettarda speciosa Nutrition 0.000 claims description 3
- 229920000715 Mucilage Polymers 0.000 claims description 3
- 102000035195 Peptidases Human genes 0.000 claims description 3
- 108091005804 Peptidases Proteins 0.000 claims description 3
- 101710118538 Protease Proteins 0.000 claims description 3
- 239000000853 adhesive Substances 0.000 claims description 3
- 235000006533 astragalus Nutrition 0.000 claims description 3
- 210000004233 talus Anatomy 0.000 claims description 3
- 244000166124 Eucalyptus globulus Species 0.000 claims description 2
- 235000013861 fat-free Nutrition 0.000 claims description 2
- 244000082204 Phyllostachys viridis Species 0.000 claims 3
- 238000000855 fermentation Methods 0.000 claims 1
- 230000004151 fermentation Effects 0.000 claims 1
- 241001330002 Bambuseae Species 0.000 abstract description 9
- 241001465754 Metazoa Species 0.000 abstract description 7
- 239000002994 raw material Substances 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 13
- 208000012659 Joint disease Diseases 0.000 description 4
- 230000037319 collagen production Effects 0.000 description 4
- 238000005227 gel permeation chromatography Methods 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 239000006227 byproduct Substances 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 208000017520 skin disease Diseases 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 235000009852 Cucurbita pepo Nutrition 0.000 description 2
- 235000015872 dietary supplement Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 241001474374 Blennius Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 208000017667 Chronic Disease Diseases 0.000 description 1
- 241000208688 Eucommia Species 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 206010023204 Joint dislocation Diseases 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000021120 animal protein Nutrition 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 230000004452 decreased vision Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 210000002683 foot Anatomy 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000003908 liver function Effects 0.000 description 1
- 235000004213 low-fat Nutrition 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 235000015277 pork Nutrition 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/40—Feeding-stuffs specially adapted for particular animals for carnivorous animals, e.g. cats or dogs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/40—Feeding-stuffs specially adapted for particular animals for carnivorous animals, e.g. cats or dogs
- A23K50/42—Dry feed
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S426/00—Food or edible material: processes, compositions, and products
- Y10S426/805—Pet food for dog, cat, bird, or fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Physiology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Botany (AREA)
- Mycology (AREA)
- Birds (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
- Fodder In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
본 발명은 박대 껍질을 이용한 저분자 콜라겐의 제조방법에 관한 것으로, 국내 원료를 사용하여 생산단가를 절감하고, 반려동물의 체내에 흡수가 용이한 저분자 콜라겐을 제조하는 박대 껍질을 이용한 저분자 콜라겐의 제조방법을 제공하기 위한 것이다. 본 발명에 따른 박대 껍질을 이용한 저분자 콜라겐의 제조방법은 건조된 박대 껍질을 물에 침지하여 핏물을 제거하는 단계, 상기 핏물을 제거한 박대 껍질을 물에 넣고 가열하여 점액질을 포함하는 불순물을 제거하는 단계, 상기 불순물을 제거한 박대 껍질에 포함되어 있는 지방을 제거하기 위해, 상기 불순물을 제거한 박대 껍질을 수산화나트륨 수용액에 침지한 후 상등액을 제거하는 단계, 상기 지방이 제거된 박대 껍질에 혼합약재 및 물을 혼합한 후, 교반 및 여과하여 콜라겐을 추출하는 단계, 상기 추출한 콜라겐을 가열살균하는 단계, 상기 가열살균한 콜라겐에 복합효소 및 히알루론산을 첨가한 후 발효시켜 저분자 콜라겐을 제조하는 단계, 상기 제조된 저분자 콜라겐에 아세톤을 혼합한 후 상등액을 제거하는 과정을 복수회 반복하여 상기 제조된 저분자 콜라겐을 세척하는 단계, 상기 세척된 저분자 콜라겐을 염화나트륨 수용액에 혼합한 후 교반하여 상기 세척된 저분자 콜라겐을 응집시키는 단계, 상기 응집된 저분자 콜라겐을 원심분리를 통해 상등액을 제거하여 저분자 콜라겐 고형물을 얻는 단계, 및 상기 저분자 콜라겐 고형물에 염화나트륨 수용액을 첨가하고 상등액을 제거한 후 여과하여 정제된 저분자 콜라겐을 제조하는 단계를 포함한다.The present invention relates to a method for manufacturing low-molecular-weight collagen using the skin of bamboo shoots, which reduces production costs by using domestic raw materials and produces low-molecular-weight collagen that is easily absorbed into the body of companion animals. It is intended to provide. The method for producing low-molecular-weight collagen using the skin of a bamboo shoot according to the present invention includes the steps of immersing the dried bamboo skin in water to remove blood, placing the blood-removed pumpkin skin in water and heating it to remove impurities including mucus. , in order to remove the fat contained in the skin of the pumpkin from which the impurities have been removed, immersing the skin of the pumpkin from which the impurities have been removed in an aqueous solution of sodium hydroxide and then removing the supernatant, adding a mixed medicine and water to the skin of the pumpkin from which the fat has been removed. After mixing, extracting collagen by stirring and filtering, heat sterilizing the extracted collagen, adding complex enzymes and hyaluronic acid to the heat-sterilized collagen and then fermenting to produce low-molecular-weight collagen, the prepared Washing the prepared low-molecular collagen by repeating the process of mixing acetone with low-molecular collagen and removing the supernatant multiple times, mixing the washed low-molecular collagen with an aqueous sodium chloride solution and stirring to agglomerate the washed low-molecular collagen. A step of centrifuging the aggregated low-molecular collagen to remove the supernatant to obtain a low-molecular-weight collagen solid, and adding an aqueous sodium chloride solution to the low-molecular-weight collagen solid, removing the supernatant, and filtering to prepare purified low-molecular-weight collagen. do.
Description
본 발명은 저분자 콜라겐의 제조방법에 관한 것으로, 더욱 상세하게는 식품 부산물인 박대 껍질로부터 추출한 콜라겐을 발효하여 저분자 콜라겐을 제조하는 박대 껍질을 이용한 저분자 콜라겐의 제조방법에 관한 것이다.The present invention relates to a method for producing low-molecular-weight collagen, and more specifically, to a method for producing low-molecular-weight collagen using the skin of a food by-product, in which low-molecular-weight collagen is produced by fermenting collagen extracted from the skin of a food by-product.
현재 국내에서 반려동물을 기르는 반려가구는 전체 가구의 29.7%를 차지하고 있다. 그에 따라 반려동물 시장이 연평균 10% 이상 성장하는 등 반려동물과 연관된 생산 및 소비 활동이 지속적으로 증가하고 있다. 특히, 반려동물을 인간으로 대하는 펫 휴머니제이션(Pet Humanization) 현상으로 반려동물의 건강을 위한 프리미엄 제품의 소비가 급증하고 있다.Currently, households raising pets in Korea account for 29.7% of all households. Accordingly, production and consumption activities related to companion animals are continuously increasing, with the companion animal market growing at an average annual rate of more than 10%. In particular, the consumption of premium products for the health of companion animals is rapidly increasing due to the phenomenon of pet humanization, which treats companion animals like humans.
그러나 서구화된 주거 문화로 반려동물의 슬개골 탈구 등의 관절질환이 발생하기 쉽다. 특히, 노령 반려동물은 관절질환 외에도 피부질환, 시력저하, 배변활동 문제, 당뇨, 고혈압 등의 만성질환이 발생할 수 있다. 이러한 질환은 조기 발견과 치료가 어렵기 때문에 식품을 통한 예방이 필요하다.However, due to the westernized residential culture, joint diseases such as patella dislocation are prone to occur in pets. In particular, in addition to joint diseases, elderly pets can develop chronic diseases such as skin diseases, decreased vision, problems with bowel movements, diabetes, and high blood pressure. These diseases are difficult to detect and treat early, so prevention through food is necessary.
콜라겐은 단백질의 일종으로서 뼈, 피부, 연골 등을 구성하는 성분으로 사람 뿐만 아니라 반려동물에게 나타나는 관절 및 피부질환에 도움을 준다. 따라서 반려동물의 관절 및 피부질환을 예방하기 위해 콜라겐을 포함하는 반려동물용 영양제의 수요가 증가하고 있다. 하지만, 현재 반려동물용 콜라겐 영양제는 대부분 고가의 수입 제품에 의존하고 있기 때문에 반려동물에게 콜라겐 영양제를 제공하는 것이 쉽지 않은 실정이다.Collagen is a type of protein that makes up bones, skin, and cartilage, and helps with joint and skin diseases not only in humans but also in pets. Therefore, the demand for nutritional supplements for pets containing collagen is increasing to prevent joint and skin diseases in pets. However, since most collagen supplements for pets currently rely on expensive imported products, it is not easy to provide collagen supplements to pets.
또한, 콜라겐은 분자량에 따라 고분자과 저분자 콜라겐으로 나눌 수 있는데, 고분자 콜라겐은 5000Da. 이상으로 분자량이 크기 때문에 체내 흡수율이 2%에 불과하다. 반면에 저분자 콜라겐은 1500Da. 이하로 분자량이 작기 때문에 체내 흡수율이 80% 이상인 것으로 알려져있다. 따라서 반려동물이 콜라겐을 용이하게 흡수할 수 있도록 콜라겐의 저분자화가 필요하다.Additionally, collagen can be divided into high molecular weight and low molecular weight collagen depending on its molecular weight, with high molecular weight collagen being 5000 Da. Because the molecular weight is large, the absorption rate in the body is only 2%. On the other hand, low molecular weight collagen is 1500Da. Because the molecular weight is small, it is known that the absorption rate in the body is over 80%. Therefore, it is necessary to reduce the molecular weight of collagen so that pets can easily absorb collagen.
따라서 본 발명의 목적은 국내 원료를 사용하여 생산단가를 절감한 박대 껍질을 이용한 저분자 콜라겐의 제조방법을 제공하기 위한 것이다.Therefore, the purpose of the present invention is to provide a method for manufacturing low-molecular-weight collagen using thinner skin, which reduces production costs by using domestic raw materials.
본 발명의 다른 목적은 반려동물의 체내에 흡수가 용이한 저분자 콜라겐을 제조하는 박대 껍질을 이용한 저분자 콜라겐의 제조방법을 제공하기 위한 것이다.Another object of the present invention is to provide a method for producing low-molecular-weight collagen using thin-molecular collagen that is easily absorbed into the body of companion animals.
상기 목적을 달성하기 위하여, 본 발명에 따른 박대 껍질을 이용한 저분자 콜라겐의 제조방법은 건조된 박대 껍질을 물에 침지하여 핏물을 제거하는 단계; 상기 핏물을 제거한 박대 껍질을 물에 넣고 가열하여 점액질을 포함하는 불순물을 제거하는 단계; 상기 불순물을 제거한 박대 껍질에 포함되어 있는 지방을 제거하기 위해, 상기 불순물을 제거한 박대 껍질을 수산화나트륨 수용액에 침지한 후 상등액을 제거하는 단계; 상기 지방이 제거된 박대 껍질에 혼합약재 및 물을 혼합한 후, 교반 및 여과하여 콜라겐을 추출하는 단계; 상기 추출한 콜라겐을 가열살균하는 단계; 상기 가열살균한 콜라겐에 복합효소 및 히알루론산을 첨가한 후 발효시켜 저분자 콜라겐을 제조하는 단계; 상기 제조된 저분자 콜라겐에 아세톤을 혼합한 후 상등액을 제거하는 과정을 복수회 반복하여 상기 제조된 저분자 콜라겐을 세척하는 단계; 상기 세척된 저분자 콜라겐을 염화나트륨 수용액에 혼합한 후 교반하여 상기 세척된 저분자 콜라겐을 응집시키는 단계; 상기 응집된 저분자 콜라겐을 원심분리를 통해 상등액을 제거하여 저분자 콜라겐 고형물을 얻는 단계; 및 상기 저분자 콜라겐 고형물에 염화나트륨 수용액을 첨가하고 상등액을 제거한 후 여과하여 정제된 저분자 콜라겐을 제조하는 단계;를 포함한다.In order to achieve the above object, the method for producing low-molecular-weight collagen using the skin of a bamboo shoot according to the present invention includes the steps of immersing the dried bamboo skin in water to remove blood; removing impurities including mucilage by placing the blood-removed pumpkin skin in water and heating it; In order to remove fat contained in the impurities-removed pumpkin skin, immersing the impurity-free pumpkin skin in an aqueous sodium hydroxide solution and then removing the supernatant; Mixing mixed herbs and water with the skin of the pumpkin from which the fat has been removed, then stirring and filtering to extract collagen; Heat sterilizing the extracted collagen; Adding complex enzymes and hyaluronic acid to the heat-sterilized collagen and then fermenting to produce low-molecular-weight collagen; Washing the prepared low-molecular collagen by mixing acetone with the prepared low-molecular collagen and then removing the supernatant, repeating the process multiple times; Mixing the washed low-molecular collagen with an aqueous sodium chloride solution and stirring to aggregate the washed low-molecular collagen; centrifuging the aggregated low-molecular collagen to remove the supernatant to obtain a low-molecular collagen solid; And adding an aqueous sodium chloride solution to the low-molecular-weight collagen solid, removing the supernatant, and then filtering to prepare purified low-molecular-weight collagen.
상기 혼합약재는 황기, 당귀, 계지, 두충, 백출, 및 우슬을 포함할 수 있다.The mixed medicine may include astragalus, angelica root, gyeji, eucalyptus, baekchul, and columbine.
상기 콜라겐을 추출하는 단계는, 상기 건조된 박대 껍질 100중량부에 대하여, 상기 혼합약재 3~3.5중량부를 첨가할 수 있다.In the step of extracting the collagen, 3 to 3.5 parts by weight of the mixed medicine may be added to 100 parts by weight of the dried pumpkin skin.
상기 복합효소는 단백질분해효소로 endo protease 및 exo protease 중 적어도 하나를 포함할 수 있다.The complex enzyme is a proteolytic enzyme and may include at least one of endo protease and exo protease.
상기 저분자 콜라겐을 제조하는 단계는, 30~60℃에서 24~48시간 동안 발효시킬 수 있다.The step of producing the low-molecular-weight collagen may be fermented at 30-60°C for 24-48 hours.
본 발명에 따른 박대 껍질을 이용한 저분자 콜라겐의 제조방법은 건조된 박대 껍질을 물에 침지하여 핏물을 제거하는 단계; 상기 핏물을 제거한 박대 껍질을 물에 넣고 95~100℃에서 10~20분 동안 가열하여 점액질을 포함하는 불순물을 제거하는 단계; 상기 불순물을 제거한 박대 껍질에 포함되어 있는 지방을 제거하기 위해, 상기 불순물을 제거한 박대 껍질을 0.05~0.15N 수산화나트륨 수용액에 18~24시간 동안 침지한 후 상등액을 제거하는 단계; 상기 지방이 제거된 박대 껍질에 혼합약재 및 물을 혼합한 후, 100~110℃에서 24~48시간 동안 교반 및 여과하여 콜라겐을 추출하는 단계; 상기 추출한 콜라겐을 100~110℃에서 가열살균하는 단계; 상기 가열살균한 콜라겐에 복합효소 및 히알루론산을 첨가한 후 30~60℃에서 24~48시간 동안 발효시켜 저분자 콜라겐을 제조하는 단계; 상기 제조된 저분자 콜라겐에 아세톤을 1:4~6의 중량비로 혼합한 후 상등액을 제거하는 과정을 복수회 반복하여 상기 제조된 저분자 콜라겐을 세척하는 단계; 상기 세척된 저분자 콜라겐을 0.01~0.2M 염화나트륨 수용액에 혼합한 후 교반하여 상기 세척된 저분자 콜라겐을 응집시키는 단계; 상기 응집된 저분자 콜라겐을 9000~11000g의 중력가속도에서 80~100분 동안 원심분리를 하여 상등액을 제거하여 저분자 콜라겐 고형물을 얻는 단계; 및 상기 저분자 콜라겐 고형물에 0.01~0.2M 염화나트륨 수용액을 첨가하고 상등액을 제거한 후 여과하여 정제된 저분자 콜라겐을 제조하는 단계;를 포함한다.The method for producing low-molecular-weight collagen using the skin of a bamboo shoot according to the present invention includes the steps of immersing the dried skin of a bamboo shoot in water to remove blood; Putting the blood-removed pumpkin skin in water and heating it at 95-100°C for 10-20 minutes to remove impurities including mucus; In order to remove the fat contained in the impurities-removed thin crust, immersing the impurities-removed thin slices in a 0.05-0.15N sodium hydroxide aqueous solution for 18-24 hours and then removing the supernatant; Extracting collagen by mixing mixed herbs and water with the fat-free skin of the pumpkin, then stirring and filtering at 100-110°C for 24-48 hours; Heat sterilizing the extracted collagen at 100-110°C; Adding complex enzymes and hyaluronic acid to the heat-sterilized collagen and then fermenting at 30-60°C for 24-48 hours to produce low-molecular-weight collagen; Washing the prepared low-molecular collagen by mixing the prepared low-molecular collagen with acetone at a weight ratio of 1:4 to 6 and then removing the supernatant, repeating the process multiple times; Mixing the washed low-molecular collagen with 0.01-0.2M aqueous sodium chloride solution and then stirring to aggregate the washed low-molecular collagen; Centrifuging the aggregated low-molecular-weight collagen for 80-100 minutes at a gravitational acceleration of 9000-11000g to remove the supernatant to obtain low-molecular-weight collagen solid; And adding 0.01 to 0.2M sodium chloride aqueous solution to the low-molecular-weight collagen solid, removing the supernatant, and then filtering to prepare purified low-molecular-weight collagen.
본 발명은 지역 특산물인 박대의 부산물을 이용하여 저분자 콜라겐을 제조함으로써 생산단가를 절감할 수 있다.The present invention can reduce production costs by manufacturing low-molecular-weight collagen using a by-product of baguette, a local specialty.
또한, 본 발명은 박대 껍질로부터 추출한 고분자 콜라겐을 복합효소 및 히알루론산을 첨가한 후 발효하여 반려동물의 체내에 흡수가 용이한 저분자 콜라겐을 제조할 수 있다.In addition, the present invention can produce low-molecular-weight collagen that is easily absorbed into the body of a companion animal by fermenting high-molecular collagen extracted from the skin of a gourd after adding complex enzymes and hyaluronic acid.
또한, 본 발명은 피부개선에 효과가 있는 약재를 첨가하여 반려동물의 피부개선에 효과가 있는 저분자 콜라겐을 제조할 수 있다.In addition, the present invention can produce low-molecular-weight collagen that is effective in improving the skin of pets by adding medicinal ingredients that are effective in improving the skin.
도 1은 본 발명에 따른 박대 껍질을 이용한 저분자 콜라겐의 제조방법을 보여주는 흐름도이다.
도 2 내지 도 7은 본 발명에 따른 박대 껍질을 이용한 저분자 콜라겐의 제조방법을 설명하기 위한 이미지이다.
도 8은 실시예에 따른 저분자 콜라겐의 GPC 분석 그래프이다.Figure 1 is a flow chart showing a method for producing low-molecular-weight collagen using thin-walled skin according to the present invention.
Figures 2 to 7 are images illustrating the method for producing low-molecular-weight collagen using thin skins according to the present invention.
Figure 8 is a GPC analysis graph of low-molecular-weight collagen according to an example.
하기의 설명에서는 본 발명의 실시예를 이해하는데 필요한 부분만이 설명되며, 그 이외 부분의 설명은 본 발명의 요지를 벗어나지 않는 범위에서 생략될 것이라는 것을 유의하여야 한다.It should be noted that in the following description, only the parts necessary to understand the embodiments of the present invention will be described, and descriptions of other parts will be omitted without departing from the gist of the present invention.
이하에서 설명되는 본 명세서 및 청구범위에 사용된 용어나 단어는 통상적이거나 사전적인 의미로 한정해서 해석되어서는 아니 되며, 발명자는 그 자신의 발명을 가장 최선의 방법으로 설명하기 위해 용어의 개념으로 적절하게 정의할 수 있다는 원칙에 입각하여 본 발명의 기술적 사상에 부합하는 의미와 개념으로 해석되어야만 한다. 따라서 본 명세서에 기재된 실시예와 도면에 도시된 구성은 본 발명의 바람직한 실시예에 불과할 뿐이고, 본 발명의 기술적 사상을 모두 대변하는 것은 아니므로, 본 출원시점에 있어서 이들을 대체할 수 있는 다양한 균등물과 변형예들이 있을 수 있음을 이해하여야 한다.The terms or words used in the specification and claims described below should not be construed as limited to their usual or dictionary meanings, and the inventor should use the concept of terminology appropriately to explain his/her invention in the best way. It must be interpreted as meaning and concept consistent with the technical idea of the present invention based on the principle that it can be defined clearly. Therefore, the embodiments described in this specification and the configurations shown in the drawings are only preferred embodiments of the present invention, and do not represent the entire technical idea of the present invention, and therefore, various equivalents can be substituted for them at the time of filing the present application. It should be understood that there may be variations.
이하, 첨부된 도면을 참조하여 본 발명의 실시예를 보다 상세하게 설명하고자 한다.Hereinafter, embodiments of the present invention will be described in more detail with reference to the attached drawings.
본 발명은 박대 껍질을 이용한 저분자 콜라겐의 제조방법을 제공한다.The present invention provides a method for producing low-molecular-weight collagen using the skin of a bamboo shoot.
도 1은 본 발명에 따른 박대 껍질을 이용한 저분자 콜라겐의 제조방법을 보여주는 흐름도이고, 도 2 내지 도 7은 본 발명에 따른 박대 껍질을 이용한 저분자 콜라겐의 제조방법을 설명하기 위한 이미지이다.Figure 1 is a flowchart showing a method for producing low-molecular-weight collagen using thin-molecular collagen according to the present invention, and Figures 2 to 7 are images for explaining the method for producing low-molecular-weight collagen using thin-molecular collagen according to the present invention.
도 1 내지 도 7을 참조하면, 먼저 S11 단계에서 건조된 박대 껍질을 물에 침지하여 핏물을 제거한다.Referring to Figures 1 to 7, first, in step S11, the dried thin skin is immersed in water to remove blood.
박대는 참서대과 생선으로 개서대, 용서대, 참서대 등의 종이 있다. 본 발명에서 사용한 박대는 군산 인근 서해안에서 나는 20~30cm의 참서대로, 함황아미노산이 많이 함유되어 피로회복 및 간 기능 개선에 도움을 주며, 지방함량이 낮아 소화가 잘되는 것으로 알려진 생선이다. 일반적으로 박대는 생으로 조리하지 않고 박대의 껍질을 벗기고 말려서 섭취하며, 벗겨낸 껍질은 버려져왔다. 버려지는 박대 껍질을 이용하여 저분자 콜라겐을 추출하는 경우, 부가가치를 창출할 수 있으며 저분자 콜라겐의 생산단가를 절감할 수 있다. Bakdae is a fish of the Chamseodae family and includes species such as Gaeseodae, Yongyongdae, and Chamseodae. The seaweed used in the present invention is a 20-30 cm long Chamseodae from the west coast near Gunsan. It is a fish known to contain a lot of sulfur-containing amino acids to help relieve fatigue and improve liver function, and to be easily digested due to its low fat content. Generally, Bakdae is not cooked raw, but is eaten by peeling and drying the skin of the Bakdae, and the peeled skin is discarded. When low-molecular-weight collagen is extracted using discarded pumpkin peels, added value can be created and the production cost of low-molecular-weight collagen can be reduced.
이러한 박대 껍질은 동물성 단백질의 일종으로 콜라겐이 풍부하게 함유되어 있다. 특히, 박대 껍질은 돈피, 닭발 등에 비해 분자량이 작은 저분자 콜라겐을 함유하고 있기 때문에 저분자 콜라겐을 추출하는데 바람직하다.The skin of this type of pumpkin is rich in collagen, a type of animal protein. In particular, gourd skin is desirable for extracting low-molecular-weight collagen because it contains low-molecular-weight collagen with a smaller molecular weight than pork skin, chicken feet, etc.
다음으로, S13 단계에서 핏물을 제거한 박대 껍질을 물에 넣고 가열하여 점액질을 포함하는 불순물을 제거한다. 이때, 95~100℃에서 10~20분 동안 가열할 수 있으며, 이에 한정되는 것은 아니다.Next, the skin of the pumpkin from which the blood was removed in step S13 is placed in water and heated to remove impurities including mucilage. At this time, it can be heated at 95 to 100°C for 10 to 20 minutes, but is not limited thereto.
다음으로, S15 단계에서 불순물을 제거한 박대 껍질에 포함되어 있는 지방을 제거하기 위해, 도 2와 같이 불순물을 제거한 박대 껍질을 수산화나트륨 수용액에 침지한 후에 상등액을 제거한다.Next, in order to remove the fat contained in the skin of the thin skin from which impurities have been removed in step S15, the thin skin from which impurities have been removed is immersed in an aqueous sodium hydroxide solution as shown in FIG. 2, and then the supernatant is removed.
수산화나트륨 수용액과 같은 염기수용액은 지방을 녹이는 성질을 갖고 있다. 이때 수산화나트륨 수용액의 농도가 높으면 지방 뿐만 아니라 콜라겐이 녹을 수 있기 때문에 0.05~0.15N의 수산화나트륨 수용액을 사용할 수 있다. 또한, 콜라겐이 파괴되지 않도록 상온에서 18~24시간 동안 침지하는 것이 바람직하다.Aqueous base solutions, such as aqueous sodium hydroxide solution, have the property of dissolving fat. At this time, if the concentration of the sodium hydroxide solution is high, not only fat but also collagen may melt, so a 0.05 to 0.15N sodium hydroxide solution can be used. Additionally, it is preferable to immerse at room temperature for 18 to 24 hours to prevent collagen from being destroyed.
이와 같이, 불순물을 제거한 박대 껍질을 수산화나트륨 수용액에 침지시키면 칼슘, 납 등의 금속성분과 지방 등의 비콜라겐 물질을 제거할 수 있다.In this way, by immersing the skin of the pumpkin from which impurities have been removed in an aqueous solution of sodium hydroxide, metal components such as calcium and lead and non-collagenous substances such as fat can be removed.
다음으로, S17 단계에서 지방이 제거된 박대 껍질에 혼합약재 및 물을 혼합한 후, 교반 및 여과하여 콜라겐을 추출한다.Next, mixed medicinal ingredients and water are mixed with the skin of the pumpkin from which fat has been removed in step S17, and then collagen is extracted by stirring and filtering.
여기서 혼합약재는 피부개선에 효과가 있는 약재로 황기, 당귀, 계지, 두충, 백출, 및 우슬을 포함할 수 있으며, 이에 한정되는 것은 아니다. 혼합약재는 건조된 박대 껍질 100중량부에 대하여, 혼합약재 3~3.5중량부를 첨가할 수 있다.Here, the mixed medicine is a medicine that is effective in improving skin and may include, but is not limited to, Astragalus, angelica root, chicken root, Eucommia, baekchul, and columbine. 3 to 3.5 parts by weight of mixed medicine can be added per 100 parts by weight of dried pumpkin peel.
S17 단계는 100~110℃에서 24~48시간 동안 교반한 후 여과하여, 도 4와 같은 콜라겐을 추출할 수 있다. 여기서 추출한 콜라겐은 고분자 콜라겐일 수 있다.In step S17, collagen can be extracted as shown in Figure 4 by stirring at 100-110°C for 24-48 hours and then filtering. The collagen extracted here may be high molecular collagen.
다음으로, S19 단계에서 추출한 콜라겐을 가열살균한다. 이때, 추출한 콜라겐은 100~110℃에서 가열살균할 수 있다.Next, the collagen extracted in step S19 is heat sterilized. At this time, the extracted collagen can be heat sterilized at 100-110°C.
다음으로, S21 단계에서 가열살균한 콜라겐에 복합효소 및 히알루론산을 첨가한 후 발효시켜, 도 6과 같은 저분자 콜라겐을 제조한다. 이때, 30~60℃에서 24~48시간 동안 발효시켜 저분자 콜라겐을 제조할 수 있으며, 이에 한정되는 것은 아니다.Next, complex enzymes and hyaluronic acid are added to the collagen heat-sterilized in step S21 and then fermented to produce low-molecular-weight collagen as shown in FIG. 6. At this time, low-molecular-weight collagen can be produced by fermenting at 30-60°C for 24-48 hours, but is not limited thereto.
여기서 복합효소는, 도 5와 같이 분말 형태의 단백질분해효소로 endo protease 및 exo protease 중 적어도 하나를 포함할 수 있으며, 이에 한정되는 것은 아니다. 복합효소는 고분자 콜라겐을 저분자 펩타이드화 하여 저분자 콜라겐을 제조할 수 있다.Here, the complex enzyme is a proteolytic enzyme in powder form as shown in Figure 5 and may include at least one of endo protease and exo protease, but is not limited thereto. Complex enzymes can produce low-molecular collagen by converting high-molecular collagen into low-molecular peptides.
히알루론산은 피부 속에 수분을 저장했다가 보습이 필요한 부위에 전달하는 역할을 하며, 콜라겐과 함께 섭취하는 경우 콜라겐의 체내 흡수율을 증가시키는 효가 있으므로 복합효소와 함께 첨가하는 것이 바람직하다. 이 뿐만 아니라, 복합효소 및 히알루론산을 첨가하는 경우, 복합효소 또는 히알루론산은 단독으로 첨가하여 발효시키는 것에 비해 콜라겐의 저분자 펩타이드화가 잘 일어날 수 있다.Hyaluronic acid plays a role in storing moisture in the skin and delivering it to areas that need moisturizing. When taken together with collagen, it has the effect of increasing the absorption rate of collagen in the body, so it is desirable to add it together with complex enzymes. In addition, when adding complex enzymes and hyaluronic acid, low-molecular-weight peptides of collagen can occur more easily than when fermenting by adding complex enzymes or hyaluronic acid alone.
다음으로, S23 단계에서 제조된 저분자 콜라겐에 아세톤을 혼합한 후 상등액을 제거하는 과정을 복수회 반복하여 제조된 저분자 콜라겐을 세척한다. 이때, 제조된 저분자 콜라겐에 아세톤을 1:4~6의 중량비로 혼합할 수 있다.Next, the low-molecular-weight collagen prepared in step S23 is mixed with acetone and the supernatant is removed by repeating the process multiple times to wash the low-molecular-weight collagen. At this time, acetone can be mixed with the prepared low-molecular-weight collagen at a weight ratio of 1:4 to 6.
다음으로, S25 단계에서 세척된 저분자 콜라겐을 염화나트륨 수용액에 혼합한 후 교반하여 세척된 저분자 콜라겐을 응집시킨다. 여기서 염화나트륨 수용액의 농도는 0.01~0.2M 일 수 있다.Next, the low-molecular-weight collagen washed in step S25 is mixed with an aqueous sodium chloride solution and then stirred to aggregate the washed low-molecular-weight collagen. Here, the concentration of the sodium chloride aqueous solution may be 0.01 to 0.2 M.
다음으로, S27 단계에서 응집된 저분자 콜라겐을 원심분리를 하여, 도 7과 같이 생성된 상등액을 제거하여 저분자 콜라겐 고형물을 얻는다. 이때, 응집된 저분자 콜라겐을 9000~11000g의 중력가속도에서 80~100분 동안 원심분리할 수 있으며, 이에 한정되는 것은 아니다.Next, the low-molecular-weight collagen aggregated in step S27 is centrifuged, and the resulting supernatant is removed as shown in FIG. 7 to obtain low-molecular-weight collagen solid. At this time, the aggregated low-molecular-weight collagen can be centrifuged for 80 to 100 minutes at a gravitational acceleration of 9,000 to 11,000 g, but is not limited to this.
마지막으로, S29 단계에서 저분자 콜라겐 고형물에 염화나트륨 수용액을 첨가하고 상등액을 제거한 후 여과하여 정제된 저분자 콜라겐을 제조한다. 이때, 염화나트륨 수용액의 농도는 0.01~0.2M 일 수 있으며, 바람직하게는 S25 단계에서 첨가한 염화나트륨 수용액의 농도보다 낮은 농도일 수 있다.Finally, in step S29, an aqueous sodium chloride solution is added to the low-molecular-weight collagen solid, the supernatant is removed, and then filtered to prepare purified low-molecular-weight collagen. At this time, the concentration of the sodium chloride aqueous solution may be 0.01 to 0.2M, and preferably may be lower than the concentration of the sodium chloride aqueous solution added in step S25.
이와 같이 제조된 저분자 콜라겐은 건조 및 분쇄하여 분말 형태로 반려동물용 영양제와 같은 분야에 활용할 수 있다.The low-molecular-weight collagen produced in this way can be dried and ground in powder form and used in fields such as nutritional supplements for pets.
[실시예 및 비교예][Examples and Comparative Examples]
이와 같은 본 발명에 따른 박대 껍질을 이용한 저분자 콜라겐의 제조방법으로 제조된 저분자 콜라겐의 특성을 확인하기 위해 실시예 및 비교예에 따른 콜라겐을 제조하였다.In order to confirm the characteristics of low-molecular-weight collagen produced by the method for producing low-molecular-weight collagen using the skin of a bamboo shoot according to the present invention, collagen was prepared according to Examples and Comparative Examples.
실시예에 따른 콜라겐의 제조방법은 아래와 같다.The method for producing collagen according to the example is as follows.
건조된 박대 껍질 1kg을 물에 침지하여 핏물을 제거한 후, 핏물을 제거한 박대 껍질을 물에 넣고 95~100℃에서 15분 동안 가열하여 점액질을 포함하는 불순물을 제거한다.After soaking 1kg of dried pumpkin skin in water to remove blood, the blood-removed pumpkin skin is placed in water and heated at 95-100°C for 15 minutes to remove impurities including mucus.
불순물을 제거한 박대 껍질에 포함된 지방을 제거하기 위해, 불순물을 제거한 박대 껍질을 0.1N 수산화나트륨 수용액에 18~24시간 동안 침지한 후 상등액을 제거한다.In order to remove the fat contained in the skin of the pumpkin from which impurities have been removed, the skin from which the impurities have been removed is immersed in a 0.1N sodium hydroxide aqueous solution for 18 to 24 hours, and then the supernatant is removed.
지방이 제거된 박대 껍질에 혼합약재 32g 및 물 3L를 혼합한 후, 105℃에서 24시간 동안 교반 및 여과하여 콜라겐을 열수추출한다.After mixing 32g of mixed medicinal ingredients and 3L of water with the skin of the pumpkin from which fat was removed, the mixture was stirred and filtered at 105°C for 24 hours to extract collagen in hot water.
추출한 콜라겐을 105℃에서 가열살균한 후, 가열살균한 콜라겐에 복합효소 및 히알루론산을 첨가한 후 30~60℃에서 24~48시간 동안 발효시켜 저분자 콜라겐을 제조한다.After the extracted collagen is heat-sterilized at 105℃, complex enzymes and hyaluronic acid are added to the heat-sterilized collagen, and then fermented at 30-60℃ for 24-48 hours to produce low-molecular-weight collagen.
제조된 저분자 콜라겐에 아세톤을 1:5의 중량비로 혼합한 후 상등액을 제거하는 과정을 복수회 반복하여 제조된 저분자 콜라겐을 세척한다.The prepared low-molecular collagen is washed by mixing acetone in a weight ratio of 1:5 with the prepared low-molecular collagen and then repeating the process of removing the supernatant several times.
세척된 저분자 콜라겐을 0.1M 염화나트륨 수용액에 혼합한 후 교반하여 세척된 저분자 콜라겐을 응집시킨다.Washed low-molecular collagen is mixed with 0.1M sodium chloride aqueous solution and stirred to aggregate the washed low-molecular collagen.
응집된 저분자 콜라겐을 10000g의 중력가속도에서 90분 동안 원심분리를 하여 상등액을 제거하여 저분자 콜라겐 고형물을 얻는다.The aggregated low-molecular-weight collagen is centrifuged at a gravity acceleration of 10,000 g for 90 minutes to remove the supernatant to obtain low-molecular-weight collagen solid.
저분자 콜라겐 고형물에 0.05M 염화나트륨 수용액을 첨가하고 상등액을 제거한 후 여과하여 정제된 저분자 콜라겐을 제조한다.A 0.05M sodium chloride aqueous solution is added to the low-molecular-weight collagen solid, the supernatant is removed, and then filtered to prepare purified low-molecular-weight collagen.
즉, 실시예에 따른 콜라겐은 박대 껍질을 24시간 동안 열수추출하고, 복합효소 및 히알루론산을 첨가하여 발효시킨 콜라겐이다.That is, the collagen according to the example is collagen obtained by extracting hot water from the skin of the pumpkin for 24 hours and adding complex enzymes and hyaluronic acid to ferment it.
제1 비교예에 따른 콜라겐은 실시예에 따른 콜라겐의 제조방법과 동일하되, 박대 껍질을 12시간 동안 열수추출하고, 발효시키지 않은 콜라겐이다.The collagen according to the first comparative example was the same as the collagen production method according to the example, but the collagen was obtained by extracting hot water from the skin of the pumpkin for 12 hours and not fermenting it.
제2 비교예에 따른 콜라겐은 실시예에 따른 콜라겐의 제조방법과 동일하되, 박대 껍질을 24시간 동안 열수추출하고, 발효시키지 않은 콜라겐이다.The collagen according to the second comparative example was the same as the collagen production method according to the example, but the collagen was obtained by extracting hot water from the skin of the pumpkin for 24 hours and not fermenting it.
제3 비교예에 따른 콜라겐은 실시예에 따른 콜라겐의 제조방법과 동일하되, 박대 껍질을 24시간 동안 열수추출하고, 히알루론산을 첨가하여 발효시킨 콜라겐이다.The collagen according to the third comparative example was the same as the collagen production method according to the example, but was obtained by extracting the skin of the pumpkin with hot water for 24 hours and adding hyaluronic acid and fermenting it.
제4 비교예에 따른 콜라겐은 실시예에 따른 콜라겐의 제조방법과 동일하되, 박대 껍질을 24시간 동안 열수추출하고, 복합효소를 첨가하여 발효시킨 콜라겐이다.The collagen according to the fourth comparative example was the same as the collagen production method according to the example, but the collagen was fermented by extracting the skin of the pumpkin with hot water for 24 hours and adding complex enzymes.
도 8은 실시예에 따른 저분자 콜라겐의 GPC(Gel Permeation Chromatography) 분석 그래프이다.Figure 8 is a GPC (Gel Permeation Chromatography) analysis graph of low-molecular-weight collagen according to an example.
도 8을 참조하면, 실시예에 따른 저분자 콜라겐은 Mw(중량평균분자량)가 626 Da. 및 Mn(수평균분자량)이 454Da.으로 저분자 콜라겐임을 확인할 수 있다.Referring to Figure 8, the low molecular weight collagen according to the example has an Mw (weight average molecular weight) of 626 Da. And Mn (number average molecular weight) is 454 Da. It can be confirmed that it is low molecular weight collagen.
이하, 비교예 및 실시예의 특성을 표 1을 참조하여 구체적으로 설명하면 다음과 같다.Hereinafter, the characteristics of comparative examples and examples will be described in detail with reference to Table 1.
아래의 표 1은 제1 내지 제4 비교예 및 실시예의 콜라겐을 GPC 분석하여 측정한 중량평균분자량을 나타낸다.Table 1 below shows the weight average molecular weight measured by GPC analysis of the collagen of Comparative Examples 1 to 4 and Examples.
표 1을 참조하면 발효시키지 않거나 히알루론산 및 복합효소 중 하나만 첨가하여 발효시킨 제1 내지 제4 비교예에 따른 콜라겐과 비교하여, 복합효소 및 히알루론산으로 발효시킨 실시예에 따른 콜라겐은 626Da.으로 작은 분자량을 갖는 것을 확인할 수 있다.Referring to Table 1, compared to the collagen according to Comparative Examples 1 to 4, which was not fermented or fermented by adding only hyaluronic acid and complex enzymes, the collagen according to the example fermented with complex enzyme and hyaluronic acid had 626 Da. It can be confirmed that it has a small molecular weight.
한편, 본 명세서와 도면에 개시된 실시예들은 이해를 돕기 위해 특정 예를 제시한 것에 지나지 않으며, 본 발명의 범위를 한정하고자 하는 것은 아니다. 여기에 개시된 실시예들 이외에도 본 발명의 기술적 사상에 바탕을 둔 다른 변형예들이 실시 가능하다는 것은, 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자에게는 자명한 것이다.Meanwhile, the embodiments disclosed in the specification and drawings are merely provided as specific examples to aid understanding, and are not intended to limit the scope of the present invention. It is obvious to those skilled in the art that in addition to the embodiments disclosed herein, other modifications based on the technical idea of the present invention can be implemented.
Claims (6)
상기 핏물을 제거한 박대 껍질을 물에 넣고 가열하여 점액질을 포함하는 불순물을 제거하는 단계;
상기 불순물을 제거한 박대 껍질에 포함되어 있는 지방을 제거하기 위해, 상기 불순물을 제거한 박대 껍질을 수산화나트륨 수용액에 침지한 후 상등액을 제거하는 단계;
상기 지방이 제거된 박대 껍질에 혼합약재 및 물을 혼합한 후, 교반 및 여과하여 콜라겐을 추출하는 단계;
상기 추출한 콜라겐을 가열살균하는 단계;
상기 가열살균한 콜라겐에 복합효소 및 히알루론산을 첨가한 후 발효시켜 저분자 콜라겐을 제조하는 단계;
상기 제조된 저분자 콜라겐에 아세톤을 혼합한 후 상등액을 제거하는 과정을 복수회 반복하여 상기 제조된 저분자 콜라겐을 세척하는 단계;
상기 세척된 저분자 콜라겐을 염화나트륨 수용액에 혼합한 후 교반하여 상기 세척된 저분자 콜라겐을 응집시키는 단계;
상기 응집된 저분자 콜라겐을 원심분리를 통해 상등액을 제거하여 저분자 콜라겐 고형물을 얻는 단계; 및
상기 저분자 콜라겐 고형물에 염화나트륨 수용액을 첨가하고 상등액을 제거한 후 여과하여 정제된 저분자 콜라겐을 제조하는 단계;
를 포함하는 박대 껍질을 이용한 저분자 콜라겐의 제조방법.Removing blood by immersing the dried pumpkin skin in water;
removing impurities including mucilage by placing the blood-removed pumpkin skin in water and heating it;
In order to remove fat contained in the impurities-removed pumpkin skin, immersing the impurity-free pumpkin skin in an aqueous sodium hydroxide solution and then removing the supernatant;
Extracting collagen by mixing mixed herbs and water with the skin of the pumpkin from which the fat has been removed, followed by stirring and filtering;
Heat sterilizing the extracted collagen;
Adding complex enzymes and hyaluronic acid to the heat-sterilized collagen and then fermenting to produce low-molecular-weight collagen;
Washing the prepared low-molecular collagen by mixing acetone with the prepared low-molecular collagen and then removing the supernatant, repeating the process multiple times;
Mixing the washed low-molecular collagen with an aqueous sodium chloride solution and stirring to aggregate the washed low-molecular collagen;
centrifuging the aggregated low-molecular collagen to remove the supernatant to obtain a low-molecular collagen solid; and
Adding an aqueous sodium chloride solution to the low-molecular-weight collagen solid, removing the supernatant, and filtering to prepare purified low-molecular-weight collagen;
A method for producing low-molecular-weight collagen using the skin of a bamboo shoot containing.
상기 혼합약재는 황기, 당귀, 계지, 두충, 백출, 및 우슬을 포함하는 것을 특징으로 하는 박대 껍질을 이용한 저분자 콜라겐의 제조방법.According to paragraph 1,
A method for producing low-molecular-weight collagen using the bark of the bamboo shoot, characterized in that the mixed medicinal ingredients include astragalus, angelica root, chicken root, eucalyptus, baekchul, and columbine.
상기 콜라겐을 추출하는 단계는,
상기 건조된 박대 껍질 100중량부에 대하여, 상기 혼합약재 3~3.5중량부를 첨가하는 것을 특징으로 하는 박대 껍질을 이용한 저분자 콜라겐의 제조방법.According to paragraph 1,
The step of extracting the collagen is,
A method for producing low-molecular-weight collagen using the skin of a pumpkin, characterized in that 3 to 3.5 parts by weight of the mixed medicine is added to 100 parts by weight of the dried pumpkin skin.
상기 복합효소는 단백질분해효소로 endo protease 및 exo protease 중 적어도 하나를 포함하는 것을 특징으로 하는 박대 껍질을 이용한 저분자 콜라겐의 제조방법.According to paragraph 1,
The complex enzyme is a proteolytic enzyme and includes at least one of endo protease and exo protease.
상기 저분자 콜라겐을 제조하는 단계는,
30~60℃에서 24~48시간 동안 발효시키는 것을 특징으로 하는 박대 껍질을 이용한 저분자 콜라겐의 제조방법.According to paragraph 1,
The step of producing the low-molecular-weight collagen is,
A method for producing low-molecular-weight collagen using pumpkin peel, characterized by fermentation at 30-60°C for 24-48 hours.
상기 핏물을 제거한 박대 껍질을 물에 넣고 95~100℃에서 10~20분 동안 가열하여 점액질을 포함하는 불순물을 제거하는 단계;
상기 불순물을 제거한 박대 껍질에 포함되어 있는 지방을 제거하기 위해, 상기 불순물을 제거한 박대 껍질을 0.05~0.15N 수산화나트륨 수용액에 18~24시간 동안 침지한 후 상등액을 제거하는 단계;
상기 지방이 제거된 박대 껍질에 혼합약재 및 물을 혼합한 후, 100~110℃에서 24~48시간 동안 교반 및 여과하여 콜라겐을 추출하는 단계;
상기 추출한 콜라겐을 100~110℃에서 가열살균하는 단계;
상기 가열살균한 콜라겐에 복합효소 및 히알루론산을 첨가한 후 30~60℃에서 24~48시간 동안 발효시켜 저분자 콜라겐을 제조하는 단계;
상기 제조된 저분자 콜라겐에 아세톤을 1:4~6의 중량비로 혼합한 후 상등액을 제거하는 과정을 복수회 반복하여 상기 제조된 저분자 콜라겐을 세척하는 단계;
상기 세척된 저분자 콜라겐을 0.01~0.2M 염화나트륨 수용액에 혼합한 후 교반하여 상기 세척된 저분자 콜라겐을 응집시키는 단계;
상기 응집된 저분자 콜라겐을 9000~11000g의 중력가속도에서 80~100분 동안 원심분리를 하여 상등액을 제거하여 저분자 콜라겐 고형물을 얻는 단계; 및
상기 저분자 콜라겐 고형물에 0.01~0.2M 염화나트륨 수용액을 첨가하고 상등액을 제거한 후 여과하여 정제된 저분자 콜라겐을 제조하는 단계;
를 포함하는 박대 껍질을 이용한 저분자 콜라겐의 제조방법.Removing blood by immersing the dried pumpkin skin in water;
Putting the blood-removed pumpkin skin in water and heating it at 95-100°C for 10-20 minutes to remove impurities including mucus;
In order to remove the fat contained in the impurities-removed thin crust, immersing the impurities-removed thin slices in a 0.05-0.15N sodium hydroxide aqueous solution for 18-24 hours and then removing the supernatant;
Extracting collagen by mixing mixed herbs and water with the fat-free skin of the pumpkin, then stirring and filtering at 100-110°C for 24-48 hours;
Heat sterilizing the extracted collagen at 100-110°C;
Adding complex enzymes and hyaluronic acid to the heat-sterilized collagen and then fermenting at 30-60°C for 24-48 hours to produce low-molecular-weight collagen;
Washing the prepared low-molecular collagen by mixing the prepared low-molecular collagen with acetone at a weight ratio of 1:4 to 6 and then removing the supernatant, repeating the process multiple times;
Mixing the washed low-molecular collagen with 0.01-0.2M aqueous sodium chloride solution and then stirring to aggregate the washed low-molecular collagen;
Centrifuging the aggregated low-molecular-weight collagen for 80-100 minutes at a gravitational acceleration of 9000-11000g to remove the supernatant to obtain low-molecular-weight collagen solid; and
Adding 0.01 to 0.2M sodium chloride aqueous solution to the low-molecular-weight collagen solid, removing the supernatant, and filtering to prepare purified low-molecular-weight collagen;
A method for producing low-molecular-weight collagen using the skin of a bamboo shoot containing.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020220112363 | 2022-09-05 | ||
| KR20220112363 | 2022-09-05 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20240033606A true KR20240033606A (en) | 2024-03-12 |
Family
ID=90299612
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020220116606A KR20240033606A (en) | 2022-09-05 | 2022-09-15 | Method for producing low-molecular-weight collagen using tongue sole skin |
| KR1020220180973A KR20240033622A (en) | 2022-09-05 | 2022-12-21 | Nutritional supplement for companion animals containing low-molecular-weight collagen using gourd skin |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020220180973A KR20240033622A (en) | 2022-09-05 | 2022-12-21 | Nutritional supplement for companion animals containing low-molecular-weight collagen using gourd skin |
Country Status (1)
| Country | Link |
|---|---|
| KR (2) | KR20240033606A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20150132650A (en) | 2014-05-15 | 2015-11-26 | 주식회사농심 | Method for preparing lowmolecular collagen peptide from fish |
-
2022
- 2022-09-15 KR KR1020220116606A patent/KR20240033606A/en unknown
- 2022-12-21 KR KR1020220180973A patent/KR20240033622A/en unknown
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20150132650A (en) | 2014-05-15 | 2015-11-26 | 주식회사농심 | Method for preparing lowmolecular collagen peptide from fish |
Also Published As
| Publication number | Publication date |
|---|---|
| KR20240033622A (en) | 2024-03-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101803770A (en) | Cooking method of giant salamander nutrient soup | |
| JP4599426B2 (en) | Method for producing health food based on garlic | |
| KR101141505B1 (en) | mathod for manufacturing pickled shrimp powder | |
| CN108576139A (en) | A kind of slim biscuit of konjaku and preparation method thereof | |
| CN104970180B (en) | A kind of technique dissolving out collagen from the flesh of fish, fish-skin, air bladder | |
| CN101366488B (en) | Concentrated seafood juice flavourings and preparation method | |
| KR101423194B1 (en) | Manufacturing Method of Soy Sauce | |
| CN111202231B (en) | Nutritional bone soup for preparing sauced beef, sauced beef and preparation method thereof | |
| KR101482857B1 (en) | Method manufacturing garnish of noodle formed by slices of boiled meat chondroichin and collagen | |
| CN107815388A (en) | A kind of shaddock vinegar and preparation method thereof | |
| CN104187971A (en) | Collagen-apple beverage and preparation method thereof | |
| KR20240033606A (en) | Method for producing low-molecular-weight collagen using tongue sole skin | |
| CN102461625A (en) | Osmanthus fragrans-bean dreg crude fibre cookies and preparation method thereof | |
| CN105285111A (en) | Health-care cheese and garlic juice beverage capable of strengthening bodies and preparation method of cheese and garlic juice beverage | |
| CN114041584A (en) | Preparation method of fish skin collagen peptide jelly capable of being stored and transported at normal temperature | |
| KR20070005954A (en) | The biofunctional dried fish and pickled fish manufacture method | |
| CN109548855A (en) | A kind of preparation method of the complex biological preservative of the extract containing pitaya peel | |
| CN106819209A (en) | A kind of preparation method of pawpaw black tea | |
| KR102568901B1 (en) | Fish cake containing fish collagen and its manufacturing method | |
| CN102754861A (en) | Sea cucumber gelatin | |
| KR101692692B1 (en) | slices of raw skate seasoned with vegetable | |
| KR102048590B1 (en) | Fabricating method of supplementary feed for black goat comprising tangle and producing method of black goat meat thereform | |
| RU2787112C1 (en) | Method for obtaining food dispersion | |
| AU2021101970A4 (en) | Instant Burdock Powder and Preparation Method Thereof | |
| CN109380673A (en) | A kind of processing method of cucumber product |