KR20230172185A - Cosmetic composition for preventing hair loss and improving hair growth - Google Patents

Abstract

The present invention relates to a cosmetic composition for preventing hair loss and improving hair growth containing brewer's yeast extract, Centella asiatica extract, and Centella asiatica extract as active ingredients. The cosmetic composition according to the present invention promotes the proliferation of human hair papilla cells and the production of hair growth factors. It can demonstrate excellent performance in suppressing the growth and production of hair loss-related factors.

Description

Cosmetic composition for preventing hair loss and improving hair growth {Cosmetic composition for preventing hair loss and improving hair growth}

The present invention relates to a cosmetic composition for preventing hair loss and improving hair growth, and more specifically, to a cosmetic composition for preventing hair loss and improving hair growth containing brewer's yeast extract, Centella asiatica extract, and Centella asiatica extract as active ingredients.

Hair loss refers to the absence of hair in areas where hair should normally exist, and generally refers to the loss of hair (thick, black hair) on the scalp. Virgin hair has no color and, unlike vellus hair, can cause cosmetic problems if it falls out.

In the case of Koreans, there are about 100,000 hairs, and it is normal for about 50 to 100 hairs to fall out per day, but if the number of hairs that fall out after sleeping or when washing the hair exceeds 100, it may be due to a pathological cause. This is high.

The cause of male pattern baldness is thought to be genetic and androgen, a male hormone, as important factors. Female pattern baldness is also presumed to occur in part through the same path as male pattern baldness, but clinically, there is a difference in its appearance. Telogen effluvium is a temporary hair loss that occurs after severe physical and mental stress such as endocrine disease, nutritional deficiency, drug use, childbirth, fever, or surgery, and occurs when part of the hair fails to complete the growth period and transitions to a telogen state and falls out.

In terms of treatment, topical medications such as minoxidil, oral medications such as finasteride and dutasteride, and hair transplant surgery are used for male-pattern hair loss and female-pattern hair loss. Recently, the use of functional cosmetics and medical devices to prevent hair loss in the early stages is increasing.

In particular, compositions for improving hair loss using various natural ingredients that are harmless to the human body are being released in functional cosmetics, such as one or more extracts selected from the group consisting of herbal extract, plantain extract, nasturtium extract, and herbacea extract, and fine needle powder. Cosmetic composition for preventing hair loss and promoting hair growth containing as an active ingredient (Public Patent Publication No. 10-2020-0014517), black garlic extract, ginseng extract, Houttuynia cordata extract, black bean extract, sorghum extract, perilla leaf extract, licorice extract , a hair cosmetic composition for preventing hair loss and improving the scalp containing natural extracts consisting of green tea extract, centella asiatica extract, aloe vera extract, and purslane extract as active ingredients (Patent Publication No. 10-2022-0057714), etc. are disclosed. .

However, the above-mentioned prior technologies have a problem in that they have little effect on preventing hair loss or promoting hair growth.

Public Patent Publication No. 10-2020-0014517 Public Patent Publication No. 10-2022-0057714

The purpose of the present invention is to prevent hair loss by proliferating human hair papilla cells containing natural substances such as brewer's yeast extract, bottle extract, and centella asiatica extract as active ingredients, increasing the production of hair growth factors, and/or suppressing the production of hair loss-related factors. The aim is to provide a cosmetic composition.

In order to achieve the above object, the present invention provides a cosmetic composition for preventing hair loss and improving hair growth containing brewer's yeast extract, Centella asiatica extract, and Centella asiatica extract as active ingredients.

In one embodiment of the present invention, the brewer's yeast extract, Centella asiatica extract, and Centella asiatica extract may be included in an amount of 0.1 to 50% by weight based on the total weight of the composition.

In one embodiment of the present invention, the brewer's yeast extract, Centella asiatica extract, and Centella asiatica extract are mixed in a quantitative ratio of 8 to 10: 0.5 to 2; It can be included at a weight ratio of 0.01 to 0.03.

In one embodiment of the present invention, the brewer's yeast extract, Centella asiatica extract, and Centella asiatica extract are mixed at 9:1; It can be included at a weight ratio of 0.025.

In one embodiment of the present invention, the cosmetic composition may have at least one selected from the group consisting of the ability to increase cell proliferation of human hair cells, the ability to increase the production of hair growth factors, and the ability to inhibit the production of hair loss inducing factors.

In one embodiment of the present invention, the brewer's yeast extract, Centella asiatica extract, and Centella asiatica quantitative extract may be obtained by hot water extraction or organic solvent extraction.

In one embodiment of the present invention, the organic solvent is methanol, ethanol, isopropanol, butanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, dimethylformamide, dimethyl sulfoxide, 1,3- It may be one or more selected from the group consisting of butylene glycol and propylene glycol.

In one embodiment of the present invention, the cosmetic composition includes hair shampoo, hair rinse, hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair treatment, hair cream, hair nutrition cream, hair moisture cream, and hair massage. It may be made of any one formulation selected from the group consisting of cream, hair wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair gel, hair mousse, and hair spray.

The cosmetic composition for preventing hair loss and improving hair growth according to the present invention can exhibit excellent performance in promoting the proliferation of human hair papilla cells, increasing the production of hair growth factors, and suppressing the production of hair loss-related factors.

Figure 1 shows a manufacturing process according to one embodiment of the brewer's yeast extract included in the cosmetic composition of the present invention.
Figure 2 shows the manufacturing process according to one embodiment of the quantitative extract of Centella asiatica included in the cosmetic composition of the present invention.
Figure 3 shows the results of measuring cell viability of human hair papilla cells (hHFDPCs) after treatment with the active ingredients included in the cosmetic composition of the present invention.
Figure 4 shows a cell staining image of human hair papilla cells (hHFDPCs) after treatment with the active ingredients included in the cosmetic composition of the present invention.
Figure 5 shows the change in expression of the growth factor (IGF-1) gene in human hair papilla cells (hHFDPCs) after treatment with the active ingredient contained in the cosmetic composition of the present invention (* p <.05, compared to the negative control group). .
Figure 6 shows the change in expression of the growth factor (VEGF) gene in human hair papilla cells (hHFDPCs) after treatment with the active ingredient contained in the cosmetic composition of the present invention (* p <.05, compared to the negative control group).
Figure 7 shows the hair loss inducing factor (TGF-) of human hair papilla cells (hHFDPCs) after treatment with the active ingredients included in the cosmetic composition of the present invention. 1) This shows the change in gene expression (* p <.05, compared to the negative control group).
Figure 8 shows the change in expression of the hair loss inducing factor (SRD5A2) gene in human hair papilla cells (hHFDPCs) after treatment with the active ingredient contained in the cosmetic composition of the present invention (* p <.05, compared to the negative control group).
Figure 9 shows the results of measuring hair fall reduction according to Example 4.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement the present invention. The present invention may be implemented in many different forms and is not limited to the embodiments described herein.

The present invention relates to a cosmetic composition for preventing hair loss and improving hair growth containing brewer's yeast extract, centella asiatica extract, and centella asiatica quantitative extract (TECA) as active ingredients.

The brewer's yeast is a strain belonging to yeast obtained during the beer fermentation process, contains amino acids, biotin, etc., and is known to be pharmacologically effective in strengthening, organizing, and producing hair.

In addition, Centella asiatica is a perennial plant belonging to the Umbrella family. It is also called gotu kola, tiger grass, clam grass, or horseshoe grass, and commonly grows in the mountains and fields of the southern islands of the Korean Peninsula. The main stem extends to the side, and there are two degenerated scale-shaped leaves near the node where the root comes from. The leaves grow densely at the nodes, are kidney-shaped, 2 to 5 cm in diameter, have a glossy surface, have dull sawtooth edges, and the length of the petiole is 4 to 20 cm. The flowers are red-purple, grow in umbels of 3 to 4 at each leaf axil, and are shaped like a head. The style is two-pronged, and the fruit is an oblong shape. Centella asiatica, a madecassol ingredient, is used as a soothing ingredient to soothe skin weakened by irritation and strengthen its self-renewal power. Centella asiatica is known to reduce dermatitis, have antibacterial, anti-inflammatory, and antioxidant effects.

로 10~20분간 가열하여 수득할 수 있으나, 이에 제한되는 것은 아니다. 도 1은 본 발명의 화장료 조성물에 포함되는 맥주효모 추출물의 일 실시예에 따른 제조 공정을 나타낸 것이다.The yeast extract and Centella asiatica extract are extracted using known extraction methods, examples of which include hot water export and solvent extraction. Here, in the case of hot water extraction, 80~121

It can be obtained by heating for 10 to 20 minutes, but is not limited thereto. Figure 1 shows a manufacturing process according to one embodiment of the brewer's yeast extract included in the cosmetic composition of the present invention.

In addition, organic solvent extraction refers to extraction using an organic solvent, water, or a mixed solvent thereof, and the organic solvent includes methanol, ethanol, isopropanol, butanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, and dichloroform. It may be an organic solvent such as methane, N, N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol, or a mixed solvent thereof, but is not limited thereto.

In addition, when extracting using such an organic solvent, the extraction conditions can be room temperature or heating. As an example, the organic solvent extraction may be an ethanol extract, preferably a 20-80% ethanol extract, and as an example, a 60% ethanol extract. The extraction method is immersed in 60% ethanol at room temperature, for example, 23°C, and then 1. It can be obtained by stirring for ~3 days.

In addition, the yeast extract and Centella asiatica extract can be used by using the extract itself, filtering the extract, fractionating the extract using a known fractionation solvent, drying the extract and dissolving the obtained powder in purified water, etc. All known methods of using extracts can be employed, and the present invention does not specifically limit this. Additionally, the extract may be concentrated or diluted, and a distillate of the extract may be used, but is not limited thereto.

The titrated extract of Centella asiatica (TECA) has been used for medicinal purposes, not only for the treatment of skin lesions such as skin redness, burns, hypertrophic scars, and eczema, but also for gastric ulcers, gastric mucosal lesions, anxiety, and improvement of venous circulation. It is also widely used for dermatological diseases.

The quantitative extract of Byeongpung is a quantitative extraction of the active ingredient from Centella asiatica plant, and its main ingredients include asiaticoside, asiatic acid, and madecassic acid. The quantitative extract of Centella asiatica differs from the Centella asiatica extract in that only the core components are extracted and quantified at high concentration and powdered.

The quantitative extract of Centella asiatica can be prepared by various extraction methods known in the art. The quantitative extract of Centella asiatica can be prepared through a process of contacting it with an extraction solvent. The centella asiatica may be dried and/or ground before the extraction step.

The extraction solvent that can be used to obtain the quantitative extract of Centella asiatica is a solvent that can be generally used in the natural product extraction process, and two or more different solvents can be mixed or used sequentially. The extraction solvent is water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol), acetone, ethyl acetate, butylacetate, dichloromethane, chloroform, hexane, 1,3-butylene glycol, or these. It may be a mixed solvent, but is not limited thereto.

More specifically, the quantitative extract of Centella asiatica includes the steps of immersing Centella asiatica in an organic solvent, decolorizing it, and then filtering the first concentration concentrate; Secondary concentration of the filtrate obtained in the filtration step to obtain crude asiaticoside, followed by purification to obtain purified asiaticoside, followed by cooling, filtration, and pulverization to obtain purified asiaticoside powder; Independently of the step of obtaining the purified asiaticoside powder, the residue obtained in the filtration step was hydrolyzed with an organic solvent to obtain crude genin compounds (Genins), and then purified and filtered and then pulverized to obtain purified genin compounds. step; and mixing the purified asiaticoside powder and the purified genin compound obtained above, but is not limited thereto. Figure 2 shows the manufacturing process according to one embodiment of the quantitative extract of Centella asiatica included in the cosmetic composition of the present invention.

In the cosmetic composition of the present invention, the active ingredient may be included in an amount of 50% by weight or less, for example, 30% or less, 20% or less, 15% or less, or 10% or less of the weight, based on the total weight of the cosmetic composition. , preferably 0.1 to 10% by weight, but is not limited thereto.

The cosmetic composition of the present invention contains the brewer's yeast extract, Centella asiatica extract, and Centella asiatica extract in an amount of 8 to 10: 0.5 to 2: 0.01 to 0.03, preferably 8 to 10: 0.5 to 1.5: 0.02 to 0.03, more preferably. 9:1; It may be included at a weight ratio of 0.025, but is not limited thereto.

In addition to the above-mentioned active ingredients, the cosmetic composition of the present invention may further include other ingredients blended in conventional cosmetics as needed. These ingredients include fat ingredients, moisturizers, emollients, surfactants, organic and inorganic ingredients. Pigments, organic powders, ultraviolet absorbers, preservatives, disinfectants, antioxidants, plant extracts, pH adjusters, alcohol, pigments, fragrances, blood circulation promoters, cooling agents, purified water, etc. may be included, but are not limited thereto.

The oil and fat components include, but are not limited to, ester-based fats, hydrocarbon-based fats, silicone-based fats, fluorine-based fats, animal fats and vegetable fats.

The ester-based oils include triglyceryl 2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, Butyl stearate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, sebacic acid. Diethyl, diisopropyl adipate, isoalkyl neopentanoate, tri(caprylic, capric acid)glyceryl, trimethylolpropane 2-ethylhexanoate, trimethylolpropane triisostearate, tetra 2-ethyl Pentaelislitol hexanoate, cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinooleate. , isostearyl laurate, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, isodecyl phosphate, 2 -Cetostearyl ethylhexanoate, 2-ethylhexanoate stearyl, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, di(caprylic, capric acid) propylene glycol, Propylene glycol dicaprylate, neopentyl glycol dicaprylate, neopentyl glycol dioctanate, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldode neopentanoate. Sil, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyl decyl isostearate, polyglycerol oleic acid ester, Polyglycerin isostearate ester, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyltriethyl citrate, acetyltri citrate Butyl, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxytearate, di2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, stearic acid. Cholesteryl, cholesteryl isostearate, hydroxystearyl cholesteryl, cholesteryl oleate, dihydrocholesteryl oleate, phytsteryl isostearate, phytsteryl oleate, 12-stealoyl hydroxy Ester systems such as isocetyl stearate, 12-stealoylhydroxystearate, and 12-stealoylhydroxystearate isostearyl are included, but are not limited thereto.

Examples of the hydrocarbon oil include squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybudene, microcrystalline wax, and petroleum jelly. It is not limited.

The silicone oils include polymethyl silicone, methylphenyl silicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane/methylcetyloxysiloxane copolymer, dimethylsiloxane/methylstealoxysiloxane copolymer, Examples include, but are not limited to, alkyl-modified silicone oil and amino-modified silicone oil.

Examples of the fluorine-based fats and oils include perfluoropolyether. Animal or plant oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, birdflower oil, soybean oil, corn oil, rapeseed oil, apricot oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil, Cottonseed oil, palm oil, cuckoo nut oil, wheat germ oil, rice germ oil, shea butter, walnut colostrum, marker damia nut oil, meadow oil, egg yolk oil, beef tallow, horse oil, mink oil, orange rape oil, jojoba oil, candelier wax. , carnaba wax, liquid lanolin, hydrogenated castor oil, and other animal or plant oils, but are not limited thereto.

The humectants include, but are not limited to, water-soluble low-molecular moisturizers, oil-soluble molecular moisturizers, water-soluble polymers, and fat-soluble polymers. Water-soluble low-molecular-weight moisturizers include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (degree of polymerization n=2 or more), poly Propylene glycol (degree of polymerization n=2 or more), polyglycerin B (degree of polymerization n=2 or more), lactic acid, lactate, etc. are included, but are not limited thereto.

The water-soluble polymers include carboxyvinyl polymer, polyaspartate, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, etc. can be mentioned. Fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymer, polyvinylpyrrolidone-hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and high-molecular silicone. Oil-soluble low-molecular moisturizers include cholesterol, cholesterol ester, etc. It may be mentioned, but it is not limited to this.

The moisturizing agent may include, but is not limited to, long-chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, and lanolin fatty acid cholesteryl ester. .

The surfactants include, but are not limited to, nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

The nonionic surfactants include self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, and POE sorbitan fatty acid ester. , POE glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hydrogenated castor oil, POE castor oil, POE·POP (polyoxyethylene·polyoxypropylene) copolymer, polyether modified silicone, lauric acid alkanolamide. , alkylamine oxide, hydrogenated soy phospholipid, etc., but is not limited thereto.

The anionic surfactant includes fatty acid soap, alpha-acylsulfonate, alkylsulfonate, alkylallylsulfonate, alkylnaphthalenesulfonate, alkylsulfate, alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, alkyl phosphate, and alkylamide phosphate. , alkyloyl alkyl taurine salt, N-acylamino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, sodium alkyl sulfoacetate, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate ester, etc. , but is not limited to this.

The cationic surfactant includes alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, and behenyltrimethylammonium bromide. , benzalkonium chloride, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salt of lanolin derivative, etc., but is not limited thereto.

The organic and inorganic pigments include silicic acid, silicic acid anhydride, magnesium silicate, talc, sericite, mica, kaolin, Bengala, clay, titanium-coated mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, bentonite, inorganic pigments such as aluminum oxide, calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine and complexes thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene/styrene copolymer. , organic pigments such as silk powder, cellulose, CI pigment yellow, CI pigment orange, and complex pigments of the above inorganic pigments and organic pigments, but are not limited thereto.

Examples of the organic powder include metal soap such as calcium stearate; Alkyl metal phosphate salts such as sodium zinc cetilate, zinc laurylate, and calcium laurylate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroyl glycine calcium; Amidesulfonic acid polyvalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylizine, N-alpha-paritoylolnithine, N-alpha-lauroylarginine, N-alpha-hydrogenated beef tallow acid acylarginine, etc. -Acyl basic amino acid; N-acyl polypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Examples include polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene/styrene copolymer, and ethylene tetrafluoride. Ultraviolet absorbers include para-aminobenzoic acid, ethyl para-aminobenzoate, amyl para-aminobenzoate, octyl para-aminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, and cinnamic acid. Benzyl, paramethoxycinnamic acid-2-ethoxyethyl, octyl paramethoxycinnamic acid, mono-2-ethylhexane glyceryl diparamethoxycinnamic acid, isopropyl paramethoxycinnamic acid, diisopropyl·diisopropylcinnamic acid ester mixture , Urocanic acid, ethyl urocanic acid, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and its salts, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone disulfonate sodium, dihydroxy Benzophenone, tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoyl methane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy) -1,3,5-triazine, 2-(2-hydroxy-5-methylphenyl)benzotriazole, etc. are included, but are not limited thereto.

The ultraviolet absorbers include para-aminobenzoic acid, ethyl para-aminobenzoate, amyl para-aminobenzoate, octyl para-aminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, Benzyl cinnamic acid, paramethoxycinnamic acid-2-ethoxyethyl, octyl paramethoxycinnamic acid, mono-2-ethylhexane glyceryl diparamethoxycinnamic acid, isopropyl paramethoxycinnamic acid, diisopropyl·diisopropyl cinnamic acid ester. Mixture, urocanic acid, ethyl urocanic acid, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and its salts, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone disulfonate sodium, dihyde Roxybenzophenone, tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoyl methane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy )-1,3,5-triazine, 2-(2-hydroxy-5-methylphenyl)benzotriazole, etc., but are not limited thereto.

Disinfectants include hinokitiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zincphyllithione, benzalkonium chloride, and photosensitizer. Examples include, but are not limited to, No. 301, mononitroguaicol sodium, and undecirenic acid.

Antioxidants include butylhydroxyanisole, propyl gallate, and elisorbic acid. pH adjusters include, but are not limited to, citric acid, sodium citrate, malic acid, sodium malate, phmalic acid, sodium phmalate, succinic acid, sodium succinate, sodium hydroxide, and sodium monohydrogen phosphate. Alcohols include higher alcohols such as cetyl alcohol, but are not limited thereto.

The ingredients that may be additionally included in the cosmetic composition of the present invention may preferably be included in an amount of 0.01 to 5% by weight, more preferably 0.01 to 3% by weight, based on the total weight of the composition, but are not limited thereto.

The cosmetic composition according to the present invention can be manufactured in various forms, such as lotion, essence, gel, emulsion, lotion, cream (oil-in-water type, water-in-oil type, multi-phase), solution, and suspension (anhydrous and aqueous). , can be prepared as anhydrous products (oil and glycol based), packs or powders.

In particular, the cosmetic composition can be manufactured in the form of a general emulsified formulation and solubilized formulation using commonly known production methods in addition to the production method specifically disclosed in the present invention. Cosmetics in the emulsified formulation include nutritional lotion, cream, essence, etc., and cosmetics in the solubilized formulation include flexible lotion. Additionally, it may be prepared in the form of an adjuvant for topical application or general application to part or the entire scalp, but is not limited thereto.

The cosmetic composition of the present invention is a specific formulation, including hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nutrition cream, hair moisture cream, and hair massage cream. , hair wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair dryer, hair preservation treatment, hair dye, hair waving agent, hair bleach, hair gel, hair glaze, hair dresser, hair It may be prepared in the form of lacquer, hair moisturizer, hair mousse or hair spray, but is not limited thereto.

In addition, suitable cosmetic formulations include, for example, solutions, gels, solid or pasty anhydrous products, emulsions obtained by dispersing the oil phase in the water phase, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes), non-ionic. It may be provided in the form of a vesicular dispersion, cream, skin, lotion, powder, ointment, spray or conceal stick. Additionally, it may be manufactured in the form of foam or in the form of an aerosol composition further containing a compressed propellant, but is not limited thereto.

The present invention will be described in more detail through the following examples, but the following examples do not limit the scope of the present invention, and should be interpreted to aid understanding of the present invention.

<Example>

Matters commonly applied to the following embodiments are as follows.

1. Cell line selection and cell culture

Human Hair Follicle Dermal Papilla cells (hHFDPCs; CEFO, Korea) were used. For culturing human hair papilla cells (hHFDPCs), a medium containing Human Dermal Papilla Basal Medium (CEFO, Korea) supplement (CEFO, Korea), penicillin, and streptomycin (CEFO, Korea) was used, 5% CO ₂ The cells were cultured under temperature conditions of 37°C in a cell incubator.

2. Setting test substance concentration

Human hair papilla cells were distributed in a 96-well plate at 1Х10 ⁴ cells/well, cultured for 24 hours, then treated with the test solution at an appropriate concentration and cultured for another 48 hours. Afterwards, WST-1 assay solution (EZ-CYTOX; DOGEN, Korea) was added at 10% of the medium volume, reacted at 37°C for an additional 0.5 to 1 hour, and then incubated with a microplate reader system (SpectraMax® i3x Multi- Absorbance was measured at 450 nm using Mode Detection Platform; Molecular Devices, USA). Reference absorbance was measured at 650 nm and the results were corrected. The results were expressed as the mean value ± standard deviation from three independent experiments.

3. Statistical analysis method

Statistical processing was performed using the Microsoft Office Excel 2013 for Windows program. To analyze the test results, three independent experiments were performed, and the average, standard deviation, and percentage were calculated for the repeated experiment results. To analyze whether cell viability changed, it was verified through Student's t-test analysis (conditions: two-tailed analysis, equal variance), and the significance of the results was indicated.

<Example 1> Measurement of human hair papilla cell proliferation

For cytotoxicity assessment of the active ingredients (breer's yeast extract + Centella asiatica extract + Centella asiatica quantitative extract) of the cosmetic composition according to the present invention, water soluble tetrazolium salt (WST-1) assay. It was measured.

Additionally, to confirm cell morphological changes, cells were stained with 0.2% crystal violet solution (Sigma, USA) and then observed using an inverted microscope (OLYMPUS, Japan).

After treating human hair papilla cells with the active ingredient at a concentration of 0 to 10% for 48 hours, WST-1 assay was performed to confirm cell viability, and the results are shown in Figures 3 and 4.

As shown in Figures 3 and 4, when the active ingredient was treated at a concentration of 10% or less (0.5, 1, 2.5, 5, 10%), the cell survival rate was 103.87%, 108.03%, and 116.00%, respectively, compared to the negative control group. , it can be seen that there is a significant increase to 116.32% and 109.48% ( p <0.05).

<Example 2> Measurement of hair growth factor gene expression level

The expression level of hair growth factor genes was measured by confirming the expression levels of IGF-1 and VEGF genes, respectively, through quantitative real-time PCR (qRT-PCR) analysis (Peter, 2008).

Human dermal fibroblasts were distributed at 5Х10 ⁵ cells/well in a 6-well culture plate and cultured for 24 hours. Afterwards, the cells were treated with a test solution of a certain concentration and further cultured for 48 hours. After harvesting the cultured cells, 1 ml TRIzol reagent (Invitrogen, USA) was added to perform cell lysis and total mRNA extraction.

The concentration and purity (A260/A280 and A260/A230 ratio) of the total mRNA were verified using a MaestroNano ^® Micro-Volume Spectrophotometer (Maestrogen, USA), and only total mRNA with a purity of 2.0 or higher was selected. Total mRNA was replaced with cDNA using M-MLV reverse transcriptase (Invitrogen, USA) and used for qRT-PCR.

qRT-PCR was performed using SYBR™ Green Master Mix (Applied Biosystems, USA), and the expression of the corresponding gene was analyzed using StepOnePlus™ Systems software (Applied Biosystems, USA).

PCR reaction conditions were denaturation at 94°C for 12 minutes, followed by denaturation (94°C, 30 seconds), annealing (60°C, 30 seconds), and polymerization (72°C, 30 seconds) for 40 cycles. Changes in gene expression were measured by comparing them with gene expression levels. The C _T value of each gene was normalized to the C _T value of β-lactin, and the relative expression level of the corresponding gene was calculated using the 2 ^-ΔΔCt method.

The results were expressed as the mean value ± standard deviation from three independent experiments. P <.05 was verified through Student's t-test, indicating the significance of the results. Primer information used in the qRT-PCR test is shown in Table 1 below.

gene forward primer reverse primer IGF-1 5'-CAGCAGTCTTCCAACCCAAT-3' 5'-CCTGCACTCCCTCTACTTGC-3' VEGF 5'-CTACCTCCACCATGCCAAGT-3' 5'-GCGAGTCTGTGTTTTTGCAG-3' β-actin 5'-GGATTCCTATGTGGGCGACGA-3' 5'-CGCTCGTGTGAGGATCTTCATG-3'

Changes in IGF-1 and VEGF mRNA expression levels were measured to determine whether the production of hair growth factors increased by treatment with the test substance, the active ingredient (breer's yeast extract + Centella asiatica extract + Centella asiatica quantitative extract). Human hair papilla cells were treated with a test solution concentration of 10% or less set as in Example 1 above and cultured for 48 hours, and then total RNA extraction and cDNA synthesis were performed.

Afterwards, the expression level of the corresponding gene mRNA was measured through qRT-PCR using the primer set and Ζβ-actin primer set for each gene shown in Table 1 above. Minoxidil was used as a positive control to improve the reliability of the test results.

As shown in Figures 5 and 6, when the test substance was treated at a concentration of 10% or less (1, 5, 10%), IGF-1 mRNA expression increased by up to 155.72 ± 9.19% and VEGF mRNA expression compared to the negative control group. It was confirmed that this increased by up to 103.69±3.08% ( p <0.05).

As a result of treating human hair papilla cells with Minoxidil (10 mM), a positive control group, for 48 hours, it was confirmed that IGF-1 and VEGF mRNA expression increased by 52.57 ± 4.85% and 32.66 ± 3.78%, respectively, compared to the negative control group ( p < 0.05).

<Example 3> Measurement of hair loss inducing factor gene expression level

The expression level of hair loss-inducing factor genes was measured by confirming the expression levels of TGF-β1 and SRD5A2 genes, respectively, through quantitative real-time PCR (qRT-PCR) analysis (Peter, 2008).

Human dermal fibroblasts were distributed at 5Х10 ⁵ cells/well in a 6-well culture plate e and cultured for 24 hours. Afterwards, the cells were treated with a test solution of a certain concentration and further cultured for 48 hours. After harvesting the cultured cells, 1 ml TRIzol reagent (Invitrogen, USA) was added to perform cell lysis and total mRNA extraction.

The concentration and purity (A260/A280 and A260/A230 ratio) of the total mRNA were verified using a MaestroNano ^® Micro-Volume Spectrophotometer (Maestrogen, USA), and only total mRNA with a purity of 2.0 or higher was selected. Total mRNA was replaced with cDNA using M-MLV reverse transcriptase (Invitrogen, USA) and used for qRT-PCR.

qRT-PCR was performed using SYBR™ Green Master Mix (Applied Biosystems, USA), and the expression of the corresponding gene was analyzed using StepOnePlus™ Systems software (Applied Biosystems, USA). PCR reaction conditions were denaturation at 94°C for 12 minutes, followed by denaturation (94°C, 30 seconds), annealing (60°C, 30 seconds), and polymerization (72°C, 30 seconds) for 40 cycles.

Changes in gene expression were measured by comparing them with gene expression levels. The Cт value of each gene was normalized to the Cт value of β-actin, and the relative expression level of the corresponding gene was calculated using the 2 ^-ΔΔCt method. The results were expressed as the mean value ± standard deviation from three independent experiments. p <.05 was verified through Student's t-test, and the significance of the results was indicated. Primer information used in the qRT-PCR test is shown in Table 2 below.

gene forward primer reverse primer TGF-β1 5'-CAGCAGTCTTCCAACCCAAT-3' 5'-CCTGCACTCCCTCTACTTGC-3' SRD5A2 5'-AAGCACACGGGAGAGCCTGAA-3' 5'-GCACCTTGTGGAATCCTGTAGC-3' β-actin 5'-GGATTCCTATGTGGGCGACGA-3' 5'-CGCTCGTGTGAGGATCTTCATG-3'

To determine whether the production of hair growth factors increased by treatment with the test substance, the active ingredient (breer's yeast extract + Centella asiatica extract + Centella asiatica quantitative extract), changes in TGF-1 and SRD5A2 mRNA expression levels were measured. Human hair papilla cells were treated with a test solution concentration of 10% or less set as in Example 1 above and cultured for 48 hours, and then total RNA extraction and cDNA synthesis were performed.

Thereafter, the expression level of the corresponding gene mRNA was measured through qRT-PCR test using the primer set and β-actin primer set for each gene shown in Table 2 above. Minoxidil was used as a positive control to improve the reliability of the test results.

As shown in Figures 7 and 8, when the active ingredient was treated at a concentration of 10% or less (1, 5, 10%), mRNA expression decreased by up to 17.74 ± 1.28% compared to the negative control group, and mRNA expression decreased by up to 29.40 ± A decrease of 2.21% was confirmed ( p <0.05).

As a result of treating human hair papilla cells with Minoxidil (10 mM), a positive control group, for 48 hours, it was confirmed that TGF-1 and SRD5A2 mRNA expression was decreased by 36.80 ± 1.81% and 52.33 ± 1.15%, respectively, compared to the negative control group ( p < 0.05).

<Example 4> Measurement of hair fall reduction

Table 3 below shows the results of measuring the number of hairs shed by having 20 adults (10 men, 10 women) use a test substance containing 10% of the active ingredient (breer's yeast extract + Centella asiatica extract + Centella asiatica quantitative extract) on 20 adults (10 men, 10 women). and shown in Figure 9. At this time, the number of hairs shed was calculated as the average value of 30 people.

division Change in the number of hairs that fall out hour Mean ± standard deviation (each) Rate of change ^a (%) Significance probability ^b ( p value) Before use 51,000±31.732 - - 2 weeks after use 26,913±14.644 -40.928 0.000† After 4 weeks of use 17,180±11.673 -61.670 0.000†

In Table 3 above, the change rate ^a (%) was calculated as [(measured value after using the product - measured value before using the product)/measured value before using the product] x 100, and the probability of significance ^b ( p value) ?? is Wilcoxon signed. p <0.05 by ranks test.

As shown in Table 3, as a result of measuring the number of hairs shed after 4 weeks of treatment, it can be seen that the number of shed hairs decreased statistically significantly both after 2 weeks of use and after 4 weeks of use compared to before use ( p < 0.05).

As the specific parts of the present invention have been described in detail above, those skilled in the art will understand that these specific techniques are merely preferred embodiments and do not limit the scope of the present invention. It will be obvious.

Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents. Simple modifications or changes of the present invention can be easily used by those skilled in the art, and all such modifications or changes can be considered to be included in the scope of the present invention.

Claims (8)

A cosmetic composition for preventing hair loss and improving hair growth containing brewer's yeast extract, centella asiatica extract, and centella asiatica extract as active ingredients.
The cosmetic composition for preventing hair loss and improving hair growth according to claim 1, comprising 0.1 to 50% by weight of the brewer's yeast extract, Centella asiatica extract, and Centella asiatica extract based on the total weight of the composition.
The method of claim 1, wherein the brewer's yeast extract, Centella asiatica extract, and Centella asiatica extract are mixed at a ratio of 8 to 10:0.5 to 2; A cosmetic composition for preventing hair loss and improving hair growth, characterized in that it contains a weight ratio of 0.01 to 0.03.
The method of claim 1, wherein the brewer's yeast extract, Centella asiatica extract, and Centella asiatica extract are mixed at a ratio of 9:1; A cosmetic composition for preventing hair loss and improving hair growth, characterized in that it contains a weight ratio of 0.025.
The method of claim 1, wherein the cosmetic composition has the ability to increase cell proliferation of human hair cells, the ability to increase the production of hair growth factors, and the ability to inhibit the production of hair loss inducing factors. Cosmetic composition for improving hair growth.
The cosmetic composition for preventing hair loss and improving hair growth according to claim 1, wherein the brewer's yeast extract, Centella asiatica extract, and Centella asiatica quantitative extract are extracted with hot water or organic solvent.
The method of claim 6, wherein the organic solvent is methanol, ethanol, isopropanol, butanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, dimethylformamide, dimethyl sulfoxide, and 1,3-butyl. A cosmetic composition for preventing hair loss and improving hair growth, comprising at least one selected from the group consisting of lene glycol and propylene glycol.
The method according to any one of claims 1 to 7, wherein the cosmetic composition is suitable for use in hair shampoo, hair rinse, hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair treatment, hair cream, and hair nutrition cream. , hair moisture cream, hair massage cream, hair wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair gel, hair mousse, and hair spray. A cosmetic composition for preventing hair loss and improving hair growth.