KR20230167176A - Composition for Improving Intestinal Microbiome Comprising Menthae Herba Extract - Google Patents

Abstract

The present invention relates to a composition that can improve intestinal microbial flora by including Menthae Herba extract as an active ingredient.
According to the present invention, the intestinal microbial flora can be effectively improved by selectively controlling the growth of beneficial and harmful intestinal bacteria using peppermint extract. Therefore, using the present invention, intestinal diseases caused by an imbalance in the intestinal microbial flora can be prevented or treated, and intestinal health can be improved to improve intestinal immunity and intestinal function.

Description

Composition for improving intestinal microbial flora comprising peppermint extract {Composition for Improving Intestinal Microbiome Comprising Menthae Herba Extract}

The present invention relates to a composition for improving the intestinal microbial flora containing a peppermint extract, and more specifically, to a composition that can effectively improve the intestinal microbial flora by selectively controlling the growth of intestinal beneficial and harmful bacteria by containing a peppermint extract. It's about.

The human intestine (intestine) has a microbial flora (microbiome) in which various microorganisms form a community. In the intestinal microbial flora, beneficial and harmful bacteria are distributed in a balanced manner, but if this balance is destroyed by external factors, it can have a harmful effect on the body. For example, it is known that long-term use of antibiotics can destroy the balance of intestinal microbial flora and increase inflammatory response by inducing excessive growth of certain pathogens.

If the imbalance in the intestinal microbial flora becomes severe due to external factors, the intestinal defense barrier function may weaken and the mucous membrane may be damaged. As a result, pathogens and toxins present in the intestinal tract stimulate the immune system, leading to inflammatory bowel disease or irritable bowel. It can lead to disease and even autoimmune disease.

As an example, the intestinal microbial flora of patients with inflammatory bowel disease has been reported to have a different composition, with harmful bacteria predominantly distributed, compared to the microbial flora of healthy people. According to Reis et al., Gut Pathogens, volume 2, 8 (2010), among the intestinal microbial flora, Escherichia sp., Yersinia sp., and Salmonella sp. are listed as commensal bacteria that have harmful effects, and in particular, it is known that harmful commensal bacteria such as Escherichia coli and Yersinia proliferate in the intestines of patients with colonic Crohn's disease, a type of inflammatory bowel disease.

However, when antibiotics are used simply to kill harmful bacteria in the intestines, not only are there controversies regarding the emergence of resistant strains, safety, and harmfulness, but antibiotics do not affect the growth of beneficial bacteria or may even inhibit the growth of beneficial bacteria, thus damaging the microbial flora. There was a problem that it was difficult to control balance effectively.

Accordingly, various medicines and functional foods are being developed to improve the intestinal microbial flora, and probiotics, a live form of beneficial bacteria, are being sold as a representative example. However, since probiotics are live bacteria, there are problems with low stability and difficulty in storage and distribution.

As an alternative to solving the problems of probiotics, prebiotics are attracting attention. Unlike probiotics, which are in the form of live bacteria, prebiotics are raw materials that serve as food for beneficial bacteria, and have the advantage of being easy to store and distribute and having the effect of promoting the growth of beneficial bacteria. In relation to this, Republic of Korea Patent Publication No. 10-2178556 describes the prebiotic activity of garlic peel extract, which promotes the growth of Enterococcus faecium and the like.

However, most prebiotic research such as the above technology is focused on promoting the growth of beneficial bacteria in the intestines, and research on raw materials that can also suppress the growth of harmful bacteria is still insufficient. Accordingly, there is a need to develop functional raw materials that can effectively improve the intestinal microbial flora by selectively controlling beneficial and harmful bacteria in the intestine.

The purpose of the present invention is to provide a composition that can effectively improve intestinal microbial flora.

Another object of the present invention is to provide a functional food for improving intestinal health containing the composition.

Another object of the present invention is to provide a pharmaceutical composition for preventing or treating inflammatory or irritable bowel disease, comprising the composition.

In order to achieve the above-described object, the present invention provides a composition for improving intestinal microbial flora containing Menthae Herba extract as an active ingredient.

In the present invention, the peppermint extract may be obtained by extracting peppermint using one or more methods selected from the group consisting of solvent extraction, supercritical extraction, and compression extraction.

In the present invention, the peppermint extract is prepared by mixing peppermint with water, methanol, ethanol, propanol, 1,3-propanediol, butanol, pentanol, hexanol, propylene glycol, dipropylene glycol, butylene glycol, glycerin, acetone, and ethyl acetate. , butyl acetate, chloroform, diethyl ether, dichloromethane, and hexane.

In the present invention, the concentration of the peppermint extract may be 0.01 to 1 g/L.

The composition of the present invention can promote the growth of beneficial intestinal bacteria and inhibit the growth of harmful intestinal bacteria.

In the present invention, the beneficial intestinal bacteria may be Lactobacillus sp.

In the present invention, the Lactobacillus genus bacteria include Lactobacillus plantarum , Lactobacillus acidophilus , Lactobacillus delbrueckii , Lactobacillus casei and Lactobacillus. It may be one or more species selected from the group consisting of Bacillus ruminis ( Lactobacillus ruminis ).

In the present invention, the intestinal harmful bacteria may be one or more of Escherichia sp. and Yersinia sp.

In the present invention, the Escherichia coli genus bacteria may be Escherichia coli, and the Yersinia genus bacteria may be one or more species selected from Yersinia enterocolitica and Yersinia pestis .

The present invention also provides a functional food for improving intestinal health, comprising the composition for improving intestinal microbial flora.

The present invention also provides a pharmaceutical composition for preventing or treating inflammatory or irritable bowel disease, comprising the composition for improving intestinal microbial flora.

In the present invention, the inflammatory bowel disease includes enterocolitis, enterogastritis, infectious diarrhea, ulcerative colitis, Crohn's disease, intestinal Behcet's disease, and inflammatory bowel disease. It may be selected from the group consisting of colon cancer (colitis-associated colorectal cancer).

According to the present invention, the intestinal microbial flora can be effectively improved by selectively controlling the growth of beneficial and harmful intestinal bacteria using peppermint extract. Therefore, using the present invention, intestinal diseases caused by an imbalance in the intestinal microbial flora can be prevented or treated, and intestinal health can be improved to improve intestinal immunity and intestinal function.

Figure 1 is a graph showing the doubling time of Lactobacillus plantarum and E. coli when treated with methanol extract of peppermint according to an embodiment of the present invention compared to the control group.
Figure 2 is a graph showing the difference in cell density depending on whether or not peppermint methanol extract was treated when Lactobacillus plantarum and E. coli were mixed cultured in an embodiment of the present invention.

Hereinafter, specific implementation forms of the present invention will be described in more detail. Unless otherwise defined, all technical and scientific terms used in this specification have the same meaning as commonly understood by a person skilled in the art to which the present invention pertains. In general, the nomenclature used herein is well known and commonly used in the art.

The present invention relates to a composition for improving intestinal microbial flora containing Menthae Herba extract.

In this specification, “microbiome” refers to a microbial community including bacteria, fungi, and other prokaryotes present in the human body, and “intestinal microbiome” refers to the microbial community present in the intestinal tract. It means microbial flora. The intestinal microbial flora includes beneficial bacteria that have beneficial effects on the intestines, such as promoting intestinal motility, enhancing intestinal immunity, and inhibiting the growth of harmful bacteria, as well as harmful bacteria that cause constipation, diarrhea, and inflammatory bowel disease.

In this specification, “improving the intestinal microbial flora” means changing the intestinal microbial flora in a direction that is beneficial to the human body. Specifically, it refers to an activity that inhibits the growth of harmful intestinal bacteria while not having a harmful effect on the beneficial intestinal bacteria. Preferably, it may refer to the activity of promoting the growth of beneficial bacteria while suppressing the growth of harmful bacteria in the intestines.

Peppermint, which is used as an active ingredient in the composition for improving the intestinal microbial flora in the present invention, is known to eliminate wind heat, have an analgesic effect, and is effective against colds, headaches, and hives, and to improve blood circulation. However, it is known to improve the intestinal microbial flora. is not known.

The present invention relates to a composition for improving intestinal microbial flora containing peppermint extract. By containing peppermint extract, the composition of the present invention can selectively control the growth of intestinal beneficial and harmful bacteria. Preferably, it can effectively improve the intestinal microbial flora by promoting the growth of intestinal beneficial bacteria while suppressing the growth of intestinal harmful bacteria. Therefore, using the present invention, intestinal health can be improved to improve intestinal immunity, intestinal function, etc., and intestinal diseases caused by an imbalance in the intestinal microbial flora can be prevented or treated.

In the present invention, peppermint extract refers to a substance obtained by extracting components of peppermint. The “extract” refers to the extract itself and the extract, such as the extract obtained by extraction treatment of herbal medicine, the diluted or concentrated liquid of the extract, the crude or purified product of the extract, the dried product obtained by drying the extract, the pulverized product of the dried product, or a mixture thereof. It is meant to include extracts of all formulations that can be formed using , and is interpreted to include crude extract and solvent fraction. The raw material subject to extraction may be the raw material itself or one that has undergone pretreatment such as grinding, drying, or steaming.

In one embodiment of the present invention, peppermint extract can be obtained using a solvent extraction method. Specifically, the extract can be prepared by adding an extraction solvent to mint, stirring, and then filtering.

In the present invention, the extraction solvent is water, methanol, ethanol, propanol, 1,3-propanediol, butanol, pentanol, hexanol, propylene glycol, dipropylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, butyl. It may include one or more selected from the group consisting of acetate, chloroform, diethyl ether, dichloromethane, and hexane. Among these, methanol can be preferably used in that it can effectively promote the growth of beneficial bacteria while suppressing the growth of harmful bacteria.

In an exemplary embodiment of the present invention, a peppermint extract can be obtained by adding and stirring 1 to 100 mL, preferably 5 to 50 mL, of extraction solvent per gram of dry peppermint. At this time, the extraction temperature may be room temperature to 100°C, preferably 40 to 60°C, and the extraction time may be 30 minutes to 10 hours, preferably 1 to 5 hours.

In one embodiment of the present invention, supercritical extraction may be used as the extraction method. Supercritical extraction is an extraction method using a supercritical fluid, which is a fluid in a state that exceeds the critical temperature and boundary pressure. Carbon dioxide can be used as the supercritical fluid, and an organic solvent can be used as an auxiliary solvent. Using the supercritical extraction method, only the extracted components can be easily obtained because the fluid vaporizes at normal pressure, and since it uses carbon dioxide, which is inert, tasteless, odorless, and harmless, it can be safely used in the manufacture of food or medicine.

In one embodiment of the present invention, it is also possible to use a compression method of extracting the raw material by applying pressure as an extraction method. For example, an extract can be prepared by pressing mint at a pressure of 100 to 2,000 kg/cm ² for 1 minute to 1 hour.

In the present invention, it is also possible to improve extraction efficiency by performing ultrasonic treatment, heat treatment, acid treatment, etc. when preparing the extract.

In the present invention, after obtaining the extract through an extraction process, one or more steps of filtering, separating, concentrating, and drying may be further performed. At this time, each step can be performed by a known method. For example, filtration may be performed by passing the extract through a filter, separation may be performed by chromatographic methods, concentration may be performed through reduced pressure distillation, and drying may be performed using freeze-drying or spray drying. It can be. Additionally, the dried extract can be prepared into powder form through a grinding step and, if necessary, resolvated with an appropriate solvent such as dimethyl sulfoxide (DMSO) or ethanol.

According to the present invention, by using a composition containing peppermint extract as an active ingredient, the microbial flora can be improved by adjusting the balance of beneficial and harmful bacteria existing in the intestine in a beneficial direction. At this time, the expression “containing as an active ingredient” means containing a sufficient amount to achieve the efficacy or activity of the peppermint extract.

In the composition of the present invention, the concentration of peppermint extract may be 0.01 to 1 g/L, preferably 0.1 to 0.8 g/L. In the above concentration range, the growth of beneficial and harmful bacteria in the intestine can be selectively controlled.

In the intestinal microbial flora, beneficial bacteria include Lactobacillus sp., Bifidobacterium sp., and Lactococcus sp., specifically Lactobacillus plantarum. ( Lactobacillus plantarum ), Lactobacillus acidophilus ( Lactobacillus acidophilus ), Lactobacillus delbrueckii , Lactobacillus casei ( Lactobacillus casei ), Lactobacillus ruminis ( Lactobacillus ruminis ), Bifidobacterium brive ( Bifidobacterium breve ), Bifidobacterium animalis , Bifidobacterium longum , Lactococcus lactis , etc.

Harmful intestinal bacteria include Escherichia sp., Yersinia sp., Salmonella sp., Shigella sp., and Clostridium sp. .), and specifically, Escherichia coli , Yersinia enterocolitica , Yersinia pestis , Salmonella enterica , and Shigella flexneri . ), Clostridium difficile , etc.

In relation to this, in the present invention, it was confirmed through examples that treatment with peppermint extract can effectively inhibit the growth of Escherichia coli, a harmful intestinal bacterium, and at the same time promote the growth of Lactobacillus plantarum, a beneficial bacterium. In this way, using the present invention, the microbial flora can be effectively improved by controlling the balance of beneficial and harmful bacteria in the intestine.

Accordingly, the present invention can also provide a functional food for improving intestinal health containing peppermint extract as an active ingredient.

When peppermint extract is applied to food according to the present invention, constipation, diarrhea, etc. can be improved by improving intestinal immunity and improving intestinal function by improving the intestinal microbial flora. Therefore, the present invention can be effectively used in functional foods that improve intestinal health.

In the present invention, functional food may include health functional food, nutritional supplements, food additives, etc.

The functional foods may include beverages, gum, tea, vitamin complexes, and health supplements, and more specifically, dairy products, confectionery products, seasonings, beverages and drink preparations, snacks, candies, jellies, ice cream and frozen desserts, and breakfast cereals. , nutritional bars, snack bars, chocolate products, processed foods, grain products and pasta, soups, sauces and dressings, confectionery products, oil and fat products, dairy drinks and milk drinks, tea, soy milk and soy dairy. -like product), frozen food, prepared food and alternative food, meat products, cheese, yogurt, bread and rolls, cakes, cookies and crackers.

Additionally, the functional food may be manufactured in dosage forms such as capsules, tablets, powders, liquid suspensions, pills, and granules containing peppermint extract.

In the present invention, the functional food may further include ingredients commonly added during food production as active ingredients in addition to peppermint extract.

The functional food of the present invention contains various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavors, colorants and thickening agents (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its salts. , organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated drinks, etc., and may contain pulp for producing fruit juice or vegetable drinks.

The present invention can also provide a pharmaceutical composition for preventing or treating intestinal diseases containing peppermint extract as an active ingredient.

In the present invention, the intestinal disease may be inflammatory bowel disease. Inflammatory bowel diseases include enterocolitis, enterogastritis, infectious diarrhea, ulcerative colitis, Crohn's disease, intestinal Behcet's disease, and inflammatory colon cancer. Associated colorectal cancer) refers to a group of intestinal diseases caused by or highly related to inflammation, and intestinal inflammation is reported to be caused by mucosal lesions caused by modification of the overall intestinal microbial flora composition rather than pathogen infection.

Alternatively, the intestinal disease may be irritable bowel disease, also called irritable bowel syndrome. It has been reported that in patients with irritable bowel disease, the diversity of microorganisms is reduced compared to the intestinal microbial flora of healthy people, and that harmful bacteria tend to increase as beneficial bacteria such as Lactobacilli decrease.

In this way, an imbalance in the intestinal microbial flora acts as a major factor in the etiology or symptoms of inflammatory bowel disease or irritable bowel disease. The present invention has the effect of improving the microbial flora by selectively controlling the growth of beneficial and harmful bacteria in the intestine by using peppermint extract, so it can be effectively used to prevent or treat such intestinal diseases.

In addition to the peppermint extract, the pharmaceutical composition may further include an appropriate pharmaceutically acceptable carrier, excipient, or diluent according to a conventional method. The pharmaceutically acceptable carriers are those commonly used in preparation, and include lactose, dextrose, sucrose, sorbitol, mannitol, starch, gum acacia, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, Includes, but is not limited to, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil.

In addition to the above components, the pharmaceutical composition of the present invention may further include lubricants, wetting agents, sweeteners, flavoring agents, emulsifiers, suspending agents, preservatives, etc. Regarding suitable pharmaceutically acceptable carriers and formulations, they can be preferably formulated according to each ingredient using the method disclosed in Remington's Pharmaceutical Sciences (19th ed., 1995).

The pharmaceutical composition of the present invention can be administered either orally or parenterally, and parenteral administration includes transdermal administration, intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, etc. Preferably, the pharmaceutical composition of the present invention can be used as an external agent and administered transdermally.

Dosage forms for oral administration include, for example, tablets, pills, hard and soft capsules, solutions, suspensions, emulsifiers, syrups, granules, etc. These dosage forms contain diluents (e.g. lactose, dextrose, water) in addition to the active ingredient. crose, mannitol, sorbitol, cellulose and/or glycine), lubricants (e.g. silica, talc, stearic acid and magnesium or calcium salts thereof and/or polyethylene glycol). Additionally, the tablets may contain binders such as magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose and/or polyvinylpyrrolidine, and in some cases starch, agar, alginic acid. or disintegrants such as sodium salts thereof or effervescent mixtures and/or absorbents, colorants, flavoring and sweetening agents. The formulation can be prepared by conventional mixing, granulating or coating methods.

In addition, representative formulations for parenteral administration are injectable formulations, and solvents for the injectable formulation include water, Ringer's solution, isotonic saline solution, or suspension. The sterile fixed oil of the injectable preparation can be used as a solvent or suspending medium, and any non-irritating fixed oil, including mono- and di-glycerides, can be used for this purpose. Additionally, the injectable preparation may use fatty acids such as oleic acid.

The composition according to the present invention is administered in a pharmaceutically effective amount. In the present invention, "pharmaceutically effective amount" means an amount sufficient to treat a disease with a reasonable benefit/risk ratio applicable to medical treatment, and the effective dose level is determined by the type, severity, and activity of the patient's disease. , can be determined based on factors including sensitivity to the drug, time of administration, route of administration and excretion rate, duration of treatment, drugs used simultaneously, and other factors well known in the field of medicine. The composition according to the present invention may be administered as an individual therapeutic agent or in combination with other therapeutic agents, may be administered sequentially or simultaneously with conventional therapeutic agents, and may be administered singly or multiple times. Considering all of the above factors, it is important to administer an amount that can achieve the maximum effect with the minimum amount without side effects, and this can be easily determined by a technician with ordinary knowledge.

Specifically, the effective amount of the composition according to the present invention may vary depending on the patient's age, gender, and weight, and is generally administered at 0.001 to 500 mg per 1 kg of body weight, preferably 0.01 to 100 mg every day or every other day, or 1 to 1 mg per day. It can be administered in three divided doses. However, since it may increase or decrease depending on the route of administration, gender, weight, age, etc., the above dosage does not limit the scope of the present invention in any way.

Because the present invention uses herbal extracts, it has higher safety compared to probiotics using live microorganisms, and can very effectively improve the microbial flora by selectively controlling the growth of beneficial and harmful bacteria in the intestines.

Example

The present invention will be described in more detail through examples below. However, these examples show some experimental methods and configurations to illustratively illustrate the present invention, and the scope of the present invention is not limited to these examples.

Preparation Example 1: Preparation of peppermint extract

30g of mint was weighed and placed in a 500mL flask, and 300mL of 95% methanol was added as an extraction solvent. It was left in a constant temperature water bath at 50°C for 3 hours and stirred once every 30 minutes, a total of 5 times. Next, it was filtered through qualitative filter paper (Whatman Filter paper No. 1) with a diameter of 90 mm, concentrated using a rotary concentrator, and then freeze-dried for 72 hours. After weighing 0.05 g of the dried extract, it was dissolved in 1 mL of DMSO to prepare a sample to be used in the experiment.

Experimental preparation: strain preculture

Lactobacillus plantarum ( L. plantarum 299v) and Escherichia coli ( E. coli O157) were used as test strains, and each bacteria was pre-cultured and then used in the experiment.

In the case of Lactobacillus plantarum, it was placed in MRS broth and cultured at 37°C at 250 rpm for 24 hours. In the case of E. coli, it was placed in Nutrient Broth and cultured overnight at 37°C and 250 rpm.

Experimental Example 1: L. plantarum and E. coli Growth regulation analysis

In order to confirm the effect of peppermint extract on controlling the growth of beneficial and harmful bacteria, the doubling time of each bacteria was measured for the peppermint extract treatment group and the control group and the results were compared.

The methanol extract prepared in Preparation Example 1 was treated with the medium at a concentration of 0.5 g/L, and pre-cultured Lactobacillus plantarum and Escherichia coli were each inoculated under the condition of Abs ₆₀₀ = 0.1. As a control, samples without extract were inoculated with bacteria in the same manner.

Samples were incubated for 8 hours, and absorbance was measured at a wavelength of 600 nm every hour. Using the recorded absorbance values, the growth curve of each bacteria over time was prepared on a log scale, the doubling time was calculated from this, and the results are shown in Figure 1 and Table 1 below. Doubling time refers to the time it takes for the number of cells to double when a microorganism grows exponentially.

strain Doubling time (min) control group peppermint extract L. plantarum 82.66 67.75 E. coli 36.85 50.41

Referring to the above experimental results, when treated with peppermint extract, the doubling time of E. coli increased by 37% and the doubling time of Lactobacillus plantarum decreased by 18% compared to the control group.

From this, it was confirmed that peppermint extract has a growth inhibitory effect on E. coli while promoting the growth of Lactobacillus plantarum.

Experimental Example 2: L. plantarum and E. coli Relative cell growth change rate analysis

In order to confirm the growth control effect of peppermint extract in an environment where beneficial and harmful bacteria coexist, the cell density of bacteria was measured for the peppermint extract treatment group and the control group and the results were compared.

The methanol extract sample according to Preparation Example 1 was treated at a concentration of 0.5 g/L in a mixed medium of LB broth and dextrose, and pre-cultured Lactobacillus plantarum and E. coli were inoculated together under the condition of Abs ₆₀₀ = 0.05, respectively. did. As a control, samples without extract were inoculated with bacteria in the same manner.

The culture was cultured at 250 rpm at 37°C for 3 hours, serial dilution was performed, and 100 μL of the diluted culture was spread on a solid agar plate. After culturing at 37°C for 24 hours, cell density (colony forming unit/mL, CFU/mL) was measured through cell counting.

Figure 2 shows the cell density (CD) measurement results of Lactobacillus plantarum (a) and E. coli (b) obtained through experiments, from which the relative cell growth change was calculated as follows. The results are shown in Table 2 below.

Relative cell growth change rate (%) = [ (CD _{Menthae Herba} - CD _control ) / CD _control ] x 100

strain Relative cell growth change rate (%) L. plantarum 6.25% E. coli -18.58%

Referring to the experimental results, when treated with peppermint extract, the cell density of beneficial bacteria was high and the cell density of harmful bacteria was low compared to the control group in an environment where Lactobacillus Plantarum, a beneficial intestinal bacteria, and Escherichia coli, a harmful bacteria, coexisted.

Through this, it was confirmed that in an environment where Lactobacillus plantarum, a beneficial intestinal bacterium, and Escherichia coli, a harmful bacterium, coexist, peppermint extract promotes the growth of beneficial intestinal bacteria while inhibiting the growth of harmful intestinal bacteria.

From the above description, those skilled in the art to which the present invention pertains will understand that the present invention can be implemented in other specific forms without changing its technical idea or essential features. In this regard, the embodiments described above should be understood in all respects as illustrative and not restrictive. The scope of the present invention should be construed as including the meaning and scope of the patent claims described below rather than the detailed description above, and all changes or modified forms derived from the equivalent concept thereof are included in the scope of the present invention.

Claims (12)

A composition for improving intestinal microbial flora containing Menthae Herba extract as an active ingredient.
According to claim 1,
A composition for improving intestinal microbial flora, wherein the peppermint extract is obtained by extracting peppermint by one or more methods selected from the group consisting of solvent extraction, supercritical extraction, and compression extraction.
According to claim 1,
The peppermint extract is mixed with water, methanol, ethanol, propanol, 1,3-propanediol, butanol, pentanol, hexanol, propylene glycol, dipropylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, butylacetate, A composition for improving intestinal microbial flora, obtained by extraction with an extraction solvent containing at least one member selected from the group consisting of chloroform, diethyl ether, dichloromethane, and hexane.
According to claim 1,
A composition for improving intestinal microbial flora, wherein the concentration of the peppermint extract is 0.01 to 1 g/L.
According to claim 1,
A composition for improving intestinal microbial flora, wherein the composition promotes the growth of beneficial intestinal bacteria and inhibits the growth of harmful intestinal bacteria.
According to claim 5,
A composition for improving intestinal microbial flora, wherein the intestinal beneficial bacteria are Lactobacillus sp.
According to claim 6,
The Lactobacillus genus bacteria are Lactobacillus plantarum , Lactobacillus acidophilus, Lactobacillus delbrueckii , Lactobacillus casei , and Lactobacillus ruminis ( A composition for improving intestinal microbial flora, which is at least one selected from the group consisting of Lactobacillus ruminis .
According to claim 5,
A composition for improving intestinal microbial flora, wherein the intestinal harmful bacteria are at least one of Escherichia sp. and Yersinia sp.
According to claim 8,
A composition for improving the intestinal microbial flora, wherein the bacteria of the Escherichia coli genus are Escherichia coli and the bacteria of the genus Yersinia are one or more species selected from Yersinia enterocolitica and Yersinia pestis . .
A functional food for improving intestinal health, comprising the composition of any one of claims 1 to 9.
A pharmaceutical composition for preventing or treating inflammatory or irritable bowel disease, comprising the composition of any one of claims 1 to 9.
According to claim 11,
The inflammatory bowel diseases include enterocolitis, enterogastritis, infectious diarrhea, ulcerative colitis, Crohn's disease, intestinal Behcet's disease, and inflammatory colon cancer. A pharmaceutical composition for preventing or treating inflammatory or irritable bowel disease, selected from the group consisting of - associated colorectal cancer.