KR102048434B1 - A composition as a prebiotic for improving intestinal microflora containing High-molecular fraction from radish leave - Google Patents

Abstract

The present invention relates to a prebiotic composition, a composition for improving intestinal microorganisms, a composition for improving intestinal health or bowel function. The growth of beneficial intestinal bacteria is introduced by containing an extract from radish leaves or a polymer fraction from the radish leaves as an active ingredient, thereby improving, preventing or treating the intestinal health or the bowel function. In addition, the composition has no toxicity, thereby being taken in a form of food.

Description

A composition as a prebiotic for improving intestinal microflora containing High-molecular fraction from radish leave}

The present invention relates to a composition for improving intestinal flora, and relates to a probiotic composition which induces proliferation of enteric beneficial bacteria by containing a radish extract or a radish polymer fraction as an active ingredient.

Prebiotics are indigestible food additives that have beneficial effects on the host by limiting enteric microorganisms or selectively promoting the growth of useful microorganisms. Oligosaccharides (fructooligosaccharides, galactooligosaccharides, isomaltooligosaccharides, xyloligosaccharides, Chitin oligosaccharides, etc.) and dietary fiber (dextrin, inulin, gum, etc.).

In the human intestine, more than 400 species of bacteria inhabit about 100 trillion grams of colon content and consist of complex ecosystems that maintain a constant balance. Intestinal flora can be divided into beneficial and harmful bacteria, the balance of which is controlled by health. To maintain good health, the intestinal flora should be maintained with many beneficial bacteria and few harmful bacteria. The flora can change depending on the human condition such as food, stress, and hormone secretion from outside, and the enzymes and metabolites they produce are involved in the metabolism of various substances in the body, absorption of nutrients or drugs, and toxic substances. Affects its creation. Therefore, the need for development of a method capable of promoting intestinal proliferation of beneficial bacteria and inhibiting harmful bacteria is gradually increasing.

Radish (Raphanus sativus Leaves) is a herbaceous or annual herb belonging to the mustard family and has been introduced in China since the Three Kingdoms era and has many indigenous species native to each province. Radish contains about 93% moisture, crude protein 1%, sugars mainly glucose by 3%, and vitamin C content of 19-39 mg%. The radish tissue contains fibrin, pectin, various minerals and amylase, which are digestive enzymes, and has unique aromatic components such as methyl mercaptane and mustard oil.

 The radish is the upper part of the radish root, that is, the stem and the leaf part, and the dried siraegi (siraegi and / or heavy; hereafter referred to as the radish) is a favorite food of most Koreans. Not only is the harvest time limited, but it is not commercialized throughout the year, so it does not play a role as a commodity like other traditional foods.

Studies on radish include pharmaceuticals for the treatment of thrombosis disease using butanol fractions of beverages (patent document 1), radish concentrates (patent document 2), dietary fiber-enhanced radish (patent document 3), and radish extracts. The composition (Patent Document 4) and the like are mainstream, and there is no known at all about the prebiotic composition which can effectively improve the total bacteria of the intestinal microorganisms.

Therefore, the present inventors have made diligent efforts to develop a new prebiotic that can effectively improve the total bacteria of intestinal microorganisms from natural products, and as a result, the present inventors have found that the extracts of the extract or the cordless polymers promote the growth of enteric beneficial bacteria and By confirming that it has the activity of inhibiting growth, the present invention has been completed.

Republic of Korea Patent Publication No. 10-1152905 Republic of Korea Patent Publication No. 10-0636366 Republic of Korea Patent Publication No. 10-0738680 Republic of Korea Patent Publication No. 10-2014-0110658

It is an object of the present invention to provide a prebiotic composition having an enteric health or bowel function improving effect using the extract as an active ingredient.

Another object of the present invention is to provide a prebiotic composition having a bowel health or bowel function improving effect, and a method for producing the same, which contain the non-molecular polymer fraction as an active ingredient.

In order to achieve the above object, the present invention provides a prebiotic composition containing the extract as an active ingredient.

The radish extract may be extracted with water, alcohol having 1 to 4 carbon atoms or a mixed solvent thereof.

The prebiotic composition may be to promote the growth of enteric beneficial bacteria.

The enteric beneficial bacteria may be strains of the genus Bifidobacterium, Lactobacillus or Streptococcus.

In addition, the present invention provides a prebiotic composition, characterized in that it contains a cheongsam polymer fraction as an active ingredient in order to achieve the other object.

The cordless polymer fraction may be a fraction of the cordless extract with a molecular weight of 10 kDa or more.

In addition, in order to achieve the above another object, the present invention provides a method for producing a cordless polymer fraction comprising the following steps.

(a) precipitating the extract to alcohol; And

(b) recovering only the polymer fraction having a molecular weight of 10 kDa or more using ultrafiltration or gel filtration chromatography.

The prebiotic composition of the present invention includes a hot water extract extracted from radish with hot water and a fraction obtained by treating it with 80% alcohol and fractionating only a polymer having a molecular weight of 10 kDa or more with an ultrafiltration membrane, thereby proliferating intestinal beneficial bacteria by prebiotics. Has the effect of promoting. Significant enhancement effects of the enteric beneficial bacteria can improve, inhibit, and prevent intestinal infection caused by pathogenic bacteria, and maintain the balance of the intestinal flora. That is, the prebiotic composition of the present invention can be utilized for improving the intestinal microorganism, the intestinal flora, the intestinal environment, and can be ingested in the form of food because there is no toxicity.

1 is a prebiotic activity score when various bacteria were treated in M9 medium containing the radish hot water extract (10 mg / ml) and FOS (fructo oligosaccharide) (10 mg / ml) of Example 1 This graph shows the analysis.
1A is for Lactobacillus paracasei subsp.tolerans (Lactobacillus paracasei subsp. Tolerans), FIG. 1B is Lactobacillus plantarum, FIG. 1C is Lactobacillus lactis NCDO604 (T) ( Lactococcus lactis subsp.lactis, FIG. 1D for Bifidobacterium longum, FIG. 1E for Bifidobacterium bifidum, FIG. 1F for Bifidobacterium breve.
FIG. 2 shows the prebiotic activity score of various microorganisms in M9 medium containing the cordless polymer fraction of Example 2 (10 mg / ml) and FOS (fructo oligosaccharides) (10 mg / ml). This graph shows the analysis.
2A is for Lactobacillus paracasei subsp.tolerans (Lactobacillus paracasei subsp. Tolerans), FIG. 2B is Lactobacillus plantarum, FIG. 2C is Lactobacillus lactis NCDO604 (T) ( Lactococcus lactis subsp.lactis, FIG. 2D for Bifidobacterium longum, FIG. 2E for Bifidobacterium bifidum, FIG. 2F for Bifidobacterium breve It is about.
Figure 3 is a graph showing the analysis of the prebiotic activity (prebiotic activity score) for the total bacteria of the cordless hot water extract, FOS (fructo oligosaccharide) of Example 1 (A) and the polymer fraction of the cordless, fraction of FOS (Example) It is a graph (B) showing the analysis of the prebiotic activity score (prebiotic activity score) for the total bacteria of lacto oligosaccharides).

The present invention relates to a probiotic composition or a composition for improving the intestinal flora, or a composition for improving intestinal health or intestinal function, by containing a radish extract or a cordless polymer fraction as an active ingredient.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated in detail.

The present invention relates to a prebiotic composition or a composition for improving intestinal flora, comprising the radish extract as an active ingredient.

Radish (Raphanus sativus Leaves) is a herbaceous or annual herb belonging to the mustard family and has been introduced in China since the Three Kingdoms era and has many indigenous species native to each province. Radish contains about 93% moisture, crude protein 1%, sugars mainly glucose by 3%, and vitamin C content of 19-39 mg%. The radish tissue contains fibrin, pectin, various minerals and amylase, which are digestive enzymes, and has unique aromatic components such as methyl mercaptane and mustard oil. The radish is the upper part of the radish root, that is, the stem and the leaf part, and the dried siraegi (siraegi and / or heavy; hereafter referred to as the radish) is a favorite food of most Koreans. Not only is the harvest time limited, but it is not commercialized throughout the year, so it does not play a role as a commodity like other traditional foods.

In the present invention, 'Prebiotic or prebiotic' is a food component that is not digested well in the body, and intestinal flora by stimulating the growth or activity of certain limited commensal bacteria without hydrolysis or absorption in the upper gastrointestinal tract. It is a substance that leads to a direction beneficial to the health of the host. Specifically, it means a substance that promotes the growth of enteric beneficial bacteria. Specifically, the prebiotics composition may also be referred to as a composition for improving intestinal microorganisms or flora. This has the effect of increasing or decreasing the efficacy of probiotics, improving intestinal health or improving intestinal function. Intestinal microorganisms or intestinal flora improvement may mean promoting the growth of enteric beneficial bacteria and suppressing the growth of enteric harmful bacteria, but may maintain the balance between enteric beneficial bacteria and harmful bacteria.

In the present invention, the 'extract' includes not only the crude extract obtained by treating the extraction solvent with the extraction raw material, but also the processed product of the crude extract. For example, the extract may be prepared in powder form by additional processes such as distillation under reduced pressure and freeze drying or spray drying.

In addition, in the present invention, the radish extract is an extract of the stem and / or leaves of the root of radish with water, an alcohol having 1 to 4 carbon atoms or a mixed solvent thereof, and the extraction method does not need to be particularly limited. Preferably, the radish extract may be a radish hot water extract or a radish ethanol aqueous solution, for example, a radish spirit extract. Most preferably, the radish extract may be a radish hot water extract.

In addition, the radish extract may be processed by repeating the radish extract, it is repeated one or more times with water, alcohol having 1 to 4 carbon atoms or a mixed solvent thereof.

The radish extract is characterized in that it promotes the growth of enteric beneficial bacteria, thereby showing an effect of improving the intestinal health or bowel function.

The radish can be cut to a certain size and dried using a shade or a dryer, and then crushed or used as it is. The drying period is not particularly limited as long as the moisture content in the radish is less than 20%.

In the present invention, the "comprising the active ingredient" or "comprising as an active ingredient" means containing an amount sufficient to achieve the enteric beneficial bacteria growth promoting effect or activity of the extract of the present invention.

As used herein, the intestinal beneficial bacterium refers to a beneficial bacterium existing in the intestine of the host and a strain that has an beneficial effect on the intestinal environment when ingested and reaches the intestine.

They survive in gastric acid and bile acids, reach the small intestine, proliferate and settle in the intestine, show useful effects in the intestine, and are non-toxic and non-pathogenic. That is, it can be understood as probiotics.

In the present invention, it was confirmed that when the composition containing the radish extract as an active ingredient, the composition has an effect of improving the intestinal flora.

The enteric beneficial bacteria may be Bifidobacterium genus, Leukonostock genus, Pediococcus genus, Weissella genus, Streptococcus genus, Lactobacillus genus and Lactococcus genus strains, but are not particularly limited thereto. Lactobacillus paracasei subsp.tolerans, Lactobacillus plantarum, Lactococcus lactis NCDO604 (T) (Lactococcus lactis subsp.lactis), Bifidobacterium longgum (Bifidobacterium longum), Bifidobacterium bifidum and Bifidobacterium breve can be any one or more selected from the group consisting of, preferably Lactobacillus paracaze ATCC 25302 (T) (Lactobacillus paracasei subsp.tolerans), Lactobacillus plantarum, Lactococcus lactis subsp.lactis and Bifido It may be a Bifidobacterium breve, and most preferably, may be Lactobacillus paracasei subsp. Tolerans (Lactobacillus paracasei subsp. Tolerans).

Lactobacillus is a generic term for microorganisms that produce energy using sugar as a nutrient source and lactic acid as a final product. Lactic acid bacteria are good for gut health because the lactic acid bacteria produce an acidic environment that prevents harmful bacteria such as Welch bacteria from living.

If the activity of lactic acid bacteria in the intestines is active, a lot of lactic acid is produced, the intestinal environment is acidified, and as a result, the activities of bacteria that are harmful to humans are suppressed.

Abnormalities occur in the body when certain harmful microorganisms are too prone to break the balance of the body. There are harmful bacteria and lactic acid bacteria in our intestines, and the ratio of lactic acid bacteria is important to keep the body healthy. This is called 'intestinal flora' and refers to the balance of intestinal bacteria.

E. coli is one of many bacteria present in the intestine of humans and animals, especially in the large intestine. Escherichia coli usually does not show pathogenicity in the intestine, but enters other parts of the intestine, causing cystitis, pyelitis, peritonitis, and sepsis. E. coli is used as an indicator of pathogenic bacteria in terms of environment and health because it is generated by manure discharged from humans and animals.

The prebiotic composition of the present invention is that the nutrient source of enteric beneficial bacteria is mixed in a single formulation, providing an optimal environment for metabolism of beneficial bacteria in the intestine at the time of ingestion, thereby promoting the growth of enteric beneficial bacteria, on the contrary, the growth of E. coli Can be easily suppressed to show a prebiotic effect.

The present invention provides a prebiotic composition characterized in that it contains a cordless polymer fraction as an active ingredient.

The prebiotics composition may also be referred to as a composition for improving enteric microorganisms or intestinal flora. This has the effect of increasing or decreasing the efficacy of probiotics, improving intestinal health or improving intestinal function. Intestinal microorganisms or intestinal flora improvement may mean promoting the growth of enteric beneficial bacteria and suppressing the growth of enteric harmful bacteria, but may maintain the balance between enteric beneficial bacteria and harmful bacteria.

In the present invention, 'fractions' are fractions obtained by additionally fractionating the radish extract, that is, the radish polymer fractions include not only those obtained by using an extraction solvent, but also those obtained by additionally applying a purification process thereto. Various methods additionally carried out, for example, fractions obtained by passing an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). It can be purified through.

The cordless polymer fraction is a fraction obtained by separating only a polymer material having a molecular weight of 10 kDa or more from the extract, and may include 45 to 60% by weight of neutral sugar and other polysaccharides relative to the total polymer fraction. In addition, the protein may be one containing 0.5 to 5% by weight further. The cordless polymer fraction may not contain dietary fiber compared to the total polymer fraction.

The neutral polysaccharide may include, but is not limited to, rhamnose, fucose, arabinose, xylose, mannose, galactose and glucose.

The method of preparing the cordless polymer fraction is as follows.

(a) precipitating the extract to alcohol; And

(b) recovering only the polymer fraction having a molecular weight of 10 kDa or more using ultrafiltration or gel filtration chromatography.

The radish extract is an extract of radish with water, alcohol having 1 to 4 carbon atoms or a mixed solvent thereof, the extraction method is not particularly limited, preferably the radish extract is a hot water extract of hot water or an aqueous solution of radish ethanol, for example It may be a barley spirit extract. Most preferably, the radish hot water extract.

More preferably, the (a) radish extract may be prepared through (a-1) hot water extraction for 2 to 5 hours at 70 to 100 ° C. At this time, the radish may be used that is not dried or dried, it may be ground or powdered.

In addition, the radish extract may be processed by repeating the radish extract, it is repeated one or more times with water, alcohol having 1 to 4 carbon atoms or a mixed solvent thereof.

The radish extract may further be carried out a process of heating for 10 to 60 minutes at 90 to 110 ℃. The heating increases the elution of the soluble polysaccharide component, and improves the polymer fraction acquisition and purity by centrifugation by denaturing and precipitating the polymer protein contained as some impurities.

The radish extract is characterized in that the ratio of the neutral polysaccharide is significantly high. That is, the radish extract extracted according to the above method contains a large amount of polysaccharide fraction.

In addition, after the step (a), it may further comprise the step of removing the solids from the known mixture, such as centrifugation, filtration, the radish extract, which can be omitted if necessary. Preferably it may be by centrifugation.

Next, in the step (b) may be to recover a fraction of 10 kDa or more molecular weight using ultrafiltration or gel filtration chromatography.

The method for obtaining the fraction is not particularly limited as long as it is a method for purifying on the basis of molecular weight, but is preferably ultrafiltration or gel filtration chromatography, and more preferably gel filtration chromatography. In addition, the final polysaccharide fraction may be in the form of extracts, concentrates, powders, and the like.

50 to 100% of the carbon atoms 1 to 4 of the final polymer fraction may be added to remove the low molecular material and impurities to further purify the polysaccharide.

Specifically, the present invention, a cordless extract obtained by extracting with water and an alcohol having 1 to 4 carbon atoms was obtained using the same method as described above to obtain a cordless polymer fraction containing only 10 kDa or more of molecular weight.

As used herein, the term 'fraction' includes not only the fraction obtained by treating the extractant but also the processed product of the cordless polymer fraction. For example, the cordless polymer fraction may be prepared in powder form by additional processes such as distillation under reduced pressure and freeze drying or spray drying.

Preferably, the cordless polymer fraction of the present invention contains 45 to 60% by weight of a neutral polysaccharide having a molecular weight of 10 kDa or more and a residual amount of other polysaccharides, and excludes glycosides (low molecular weight material) of 10 kDa or less through a filtration process.

Promoting the proliferation of lactic acid bacteria in prebiotics is known to be mainly due to monosaccharides. However, despite the removal of low molecular weight substances (glycosides) having a molecular weight of 10 kDa or less, the cordless polymer fraction of the present invention was found to have a growth-promoting effect or activity against the majority of enteric beneficial bacteria, as well as the Lactobacillus paracaze ATCC 25302 (T) (Lactobacillus paracasei subsp.tolerans), Lactobacillus plantarum, Lactococcus lactis subsp.lactis, Lactococcus lactis subsp.lactis, Bifidobacterium longum, Bifidobacterium longum It was confirmed that the Bifidobacterium bifidum and Bifidobacterium breve achieve a level of prebiotic activity score similar to that of fructo oligosaccharide (FOS) (FIG. 2). Especially for Lactobacillus paracasei ATCC 25302 (T) (Lactobacillus paracasei subsp.tolerans), Lactobacillus plantarum and Lactococcus lactis NCDO604 (T) (Lactococcus lactis subsp.lactis) It was confirmed that the prebiotic activity score was higher than 1.5 times higher than that of the hydrothermal extract (FIG. 2).

The broad range of the cordless polymer fraction of the present invention has the meaning of including the cordless polymer fraction, the cordless polysaccharide fraction, the cordless polymer processed material, such as the cordless polymer powder, which are formulated to be administered to animals. Although experiments were carried out with the cordless polymer fraction in the present invention, it will be expected to those skilled in the art that the desired effect can be achieved in the same form as the cordless polymer workpiece.

On the other hand, the term 'containing as an active ingredient' in the present specification means containing an amount sufficient to achieve the efficacy or activity of the cordless polymer fraction. In one example, the cordless polymer fraction is used at a concentration of 10 to 1500 μg / ml, preferably 100 to 1000 μg / ml. Since the cordless polymer fraction has no adverse effects on the human body even when excessively administered as a natural product, the upper limit of the amount of the cordless polymer fraction contained in the composition of the present invention may be carried out by those skilled in the art.

Another aspect of the present invention relates to a composition for improving intestinal health or improving bowel function, characterized in that it contains a radish extract or a radish polymer fraction as an active ingredient.

In the present invention, the radish extract or radish polymer fraction has an effect of improving intestinal health or improving intestinal function by promoting the promotion of enteric beneficial bacteria.

The present invention is enteric beneficial bacteria (Lactobacillus paracasei subsp. Tolerans), Lactobacillus plantarum, Lactobacillus plantarum, Lactococcus lactis subsp. Lactis , Bifidobacterium longum, Bifidobacterium bifidum, and Bifidobacterium breve, and at least one selected from the group consisting of) In contrast, by confirming that the growth of Escherichia coli (Escherichia coli) is inhibited, it was confirmed that the intestinal health and intestinal function improved.

In particular, when the extract was used as an active ingredient, it showed the same level of prebiotic activity with respect to the entire strains of conventional fructo oligosaccharides, in particular, Lactobacillus paracasei ATCC 25302 (T) (Lactobacillus paracasei subsp. Tolerans), lactose Lactobacillus plantarum, Lactococcus lactis subsp.lactis and / or Bifidobacterium breve were found to have the highest activity, and the cordless polymer fraction Lactobacillus paracasei ATCC 25302 (T), Lactobacillus plantarum, Lactobacillus plantarum, Lactococcus lactis NCDO604 (T) (Lactococcus) lactis subsp. lactis) was found to achieve significantly higher activity more than 1.5 times.

That is, when the extract of the present invention or the extract of the cordless polymer fraction is treated with various intestinal beneficial bacteria known to have an effect of improving intestinal health or improving intestinal function, it promotes the growth of the intestinal beneficial bacteria, thereby improving intestinal health or intestinal function It can be used for suppression or prevention purposes.

The composition of the present invention is a pharmaceutical composition, and can be prepared using a pharmaceutically acceptable and physiologically acceptable adjuvant in addition to the extract or the extract of the polymer extract as an active ingredient, the adjuvant, excipients, disintegrants, sweeteners, Binders, coatings, swelling agents, lubricants, lubricants, flavoring agents, and the like can be used.

The pharmaceutical composition may be preferably formulated into a pharmaceutical composition comprising one or more pharmaceutically acceptable carriers in addition to the active ingredient described above for administration.

Formulation forms of the pharmaceutical composition may be granules, powders, tablets, coated tablets, capsules, suppositories, solutions, syrups, juices, suspensions, emulsions, drops or injectable solutions. For example, for formulation in the form of tablets or capsules, the active ingredient may be combined with an oral, nontoxic, pharmaceutically acceptable inert carrier such as ethanol, glycerol, water and the like. In addition, if desired or necessary, suitable binders, lubricants, disintegrants and coloring agents may also be included in the mixture. Suitable binders include, but are not limited to, natural and synthetic gums such as starch, gelatin, glucose or beta-lactose, corn sweeteners, acacia, trackercance or sodium oleate, sodium stearate, magnesium stearate, sodium Benzoate, sodium acetate, sodium chloride and the like. Disintegrants include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum, and the like.

Acceptable pharmaceutical carriers in compositions formulated in liquid solutions are sterile and physiologically compatible, including saline, sterile water, Ringer's solution, buffered saline, albumin injectable solutions, dextrose solution, maltodextrin solution, glycerol, ethanol and One or more of these components may be mixed and used, and other conventional additives such as antioxidants, buffers and bacteriostatic agents may be added as necessary. Diluents, dispersants, surfactants, binders and lubricants may also be added in addition to formulate into injectable formulations, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like.

Furthermore, the method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA can be formulated according to each disease or component according to the appropriate method in the art.

The pharmaceutical composition of the present invention may be administered orally or parenterally, and in the case of parenteral administration, it may be administered by intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, transdermal administration, and preferably, oral administration. .

Suitable dosages of the pharmaceutical compositions of the invention vary depending on factors such as the formulation method, mode of administration, age, weight, sex, morbidity, condition of food, time of administration, route of administration, rate of excretion and response to reaction, Usually a skilled practitioner can easily determine and prescribe a dosage effective for the desired treatment or prophylaxis. According to a preferred embodiment of the present invention, the daily dose of the pharmaceutical composition of the present invention is 0.001-10 g / kg.

The pharmaceutical compositions of the present invention may be prepared in unit dose form or formulated into pharmaceutically acceptable carriers and / or excipients or incorporated into multi-dose containers. In this case, the formulation may be in the form of a solution, suspension or emulsion in an oil or an aqueous medium, or may be in the form of extracts, powders, granules, tablets or capsules, and may further include a dispersant or stabilizer.

In another example, the above-described extract or cordless polymer fraction as described above may be used in various ways as a food composition for improving or inhibiting bowel health or bowel function.

The food composition comprising the extract of the present invention or the extract of the cheongsam as an active ingredient can be used in the form of various foods, for example, beverages, gum, tea, vitamin complex, powder, granules, tablets, capsules.

The food composition according to the present invention may be formulated in the same manner as the pharmaceutical composition, used as a functional food, or added to various foods. Examples of the food to which the composition of the present invention may be added include beverages, alcoholic beverages, confectionary, diet bars, dairy products, meat, chocolates, pizza, ramen noodles, other noodles, gums, ice creams, vitamin complexes, and health supplements. Etc.

The food composition of the present invention may include, as an active ingredient, radish extracts or radish polymer fractions, as well as components commonly added in food preparation, and include, for example, proteins, carbohydrates, fats, nutrients, seasonings and flavoring agents. It includes. Examples of the above carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And sugars such as conventional sugars such as polysaccharides such as dextrin, cyclodextrin and the like and xylitol, sorbitol, erythritol. As the flavoring agent, natural flavoring agents (tauumatin, stevia extract (for example rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used. For example, when the food composition of the present invention is prepared with a drink and a beverage, the citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, fruit juice, and various plant extracts, etc., are additionally included in addition to the extract or the cordless polymer fraction of the present invention. You can.

The present invention provides a health functional food comprising a food composition for improving, suppressing or preventing intestinal health or intestinal function, which comprises the above-mentioned extract of radish or polymerless as an active ingredient. Health functional foods are foods prepared by adding radish extracts or cordless polymer fractions to food materials such as beverages, teas, spices, gums and confectionery, or by encapsulating, powdering, and suspensions. Means to bring, but unlike the general medicine has the advantage that there are no side effects that can occur when taking a long-term use of the drug as a raw material. The health functional food of the present invention thus obtained is very useful because it can be consumed on a daily basis. The amount of addition of the extract of the radish or the polymer of the radish polymer in the health functional food cannot be defined uniformly depending on the type of the health functional food, but it is sufficient to add it in a range that does not impair the original taste of the food. It is usually in the range of 0.01 to 50% by weight, preferably 0.1 to 20% by weight. In addition, in the case of a health functional food in the form of pills, granules, tablets or capsules, it is usually added in the range of 0.1 to 100% by weight, preferably 0.5 to 80% by weight. In one embodiment, the dietary supplement of the present invention may be in the form of pills, tablets, capsules or beverages.

The present invention also provides the use of the extract or the cordless polymer fraction for the manufacture of a medicament or food for improving, inhibiting or preventing intestinal health or bowel function. As described above, the extract or the cordless polymer fraction may be used for the purpose of improving, preventing or treating intestinal health or bowel function.

The present invention also provides a method for improving, preventing or treating intestinal health or intestinal function, comprising administering to a mammal an effective amount of the extract or the cordless polymer fraction.

As used herein, the term "mammal" refers to a mammal that is the subject of treatment, observation or experimentation, preferably human.

As used herein, the term “effective amount” means an amount of an active ingredient or pharmaceutical composition that induces a biological or medical response in a tissue system, animal or human, as contemplated by a researcher, veterinarian, doctor or other clinician, Includes amounts that induce relief of symptoms. The effective amount and frequency of administration for the active ingredient of the present invention may vary depending on the desired effect. Therefore, the optimal dosage to be administered can be readily determined by one skilled in the art and can be determined by the person skilled in the art, including the level of enteric health or bowel function, the amount of active ingredients and other ingredients contained in the composition, the type of formulation, and the age, weight, general health of the patient. , Sex and diet, time of administration, route of administration and rate of composition, duration of treatment, and drugs used concurrently. In the prophylactic, therapeutic or ameliorating method of the present invention, for adults, it is preferable to administer the extract or the cordless polymer fraction at a dose of 0.001 g / kg to 10 g / kg when administered once to several times a day.

In the treatment method of the present invention, the composition containing the extract or the extract of the polymer as an active ingredient in the oral, rectal, intravenous, intraarterial, intraperitoneal, intramuscular, intrasternal, transdermal, topical, intraocular or intradermal route It may be administered in a conventional manner.

Hereinafter, preferred examples are provided to aid the understanding of the present invention, but the following examples are merely for exemplifying the present invention, and it will be apparent to those skilled in the art that various changes and modifications can be made within the scope and spirit of the present invention. It is natural that such variations and modifications fall within the scope of the appended claims.

Example 1 Radish Hot Water Extract

Domestic Mucheong produced by Wanju Agricultural Cooperatives was purchased and used. The radish was re-dried for 24 hours in a 50 ° C. drying oven, and then pulverized to 1.0 mm or less with a grinder to prepare a radish powder.

Distilled water corresponding to 20-fold volume was added to 5 g of radish powder, and reflux extraction was performed at 80 ° C. for 3 hours to obtain a hot water extract. The hot water extract was filtered, concentrated under reduced pressure using a rotary vacuum evaporator, and lyophilized for 72 hours. The radish hot water extract obtained by the above method was described as Water-soluble extract from radish leaves (WERL).

Example 2 cordless polymer fraction

Domestic Mucheong produced by Wanju Agricultural Cooperatives was purchased and used. The radish was re-dried for 24 hours in a 50 ° C. drying oven, and then pulverized to 1.0 mm or less with a grinder to prepare a radish powder.

Distilled water corresponding to 20-fold volume was added to 100 g of the radish powder, and the mixture was extracted under reflux for 3 hours at a temperature of 80 ° C. to obtain a hydrothermal extract. The hot water extract was filtered and concentrated using a rotary vacuum evaporator to reduce the pressure to 1/10 of the extract, and 80% alcohol corresponding to 4 times the volume (v / v) of the concentrated hot water extract. Was added to the hot water extract and precipitated for 24 hours. The precipitate was obtained by centrifugation (6000 rpm, 635 kg, 30 minutes), and a small amount of distilled water was added thereto to redissolve the precipitate and dialyzed membrane (MW cut off 12,000, Sigma-Aldrich Co., St. Louis, MO, USA) was used for 2 to 3 days in dialysis to remove low molecular weight material and freeze-drying for 72 hours to obtain a cordless polymer fraction. The cordless polymer fraction obtained by the above method was named HFRL (High-molecular fraction from radish leaves).

<Test Example>

Test Example 1 Analysis of yield, sugar and protein content of the hydrothermal extract of Example 1 and the polymer fraction of Example 2

The constituent sugar analysis was modified by Albersheim et al. By hydrolysis and derivatization of each constituent sugar with altitol acetate and aldonolactone, followed by GC (Gas Chromatography ACME-6100, Young-Lin Co. Ltd. Anyang, Korea). ). The sample of Example 1 or Example 2 was hydrolyzed by reaction at 121 ° C. for 1.5 hr in 2 M trifluroacetic acid (TFA), and then dissolved in 1 ml of 1 M NH ₄ OH (ammonia solution) to 10 mg of NaBH _4. 4 hr reduction. Acetic acid was added to remove residual NaBH ₄ , and methanol and methanol were added and dried repeatedly to remove excess acetic acid, thereby converting to alditol corresponding to each component.

To determine the composition of the neutral sugar, 1 ml of acetic anhydride was added to each of the altitols, and then reacted for 30 min at 121 ° C. to convert to the altitol acetate, which was converted to chloroform / H ₂ O. The mixture was extracted with a two-phase solvent system, and the extract was dissolved in a small amount of acetone after drying, and used as a sample for GC analysis.

Protein quantitation was measured according to the Bradford method, and diluted to a constant multiple of 1 mg / ml using BSA (Bovine Serum Albumin, sigma aldrich) as a standard. 20 μl of a standard and a sample were added to 980 μl of Bradford reagent, and the absorbance was measured at 595 nm using a microplate reader. Protein quantification was converted to amounts for BSA.

The total sugar was measured by phenol-sulfuric acid method. After adding 0.5% of the sample solution, the same amount of 5% phenol solution was added and stirred, and 2.5 mL of concentrated sulfuric acid (98%, v / v) was added and reacted at room temperature for 20 minutes. The absorbance was measured at 470 nm using a reader. Quantitative determination of total sugar was made by using the glucose standard (sigma aldrich) and the calibration curve was converted into the amount for this.

As a result, as a result of analyzing the physicochemical characteristics such as the main component content of the hydrothermal extract and polymer fraction from radish was as shown in Table 1.

division Example 1 (WERL) Example 2 (HFRL) Extraction yield (%) 46.63 1.85         Chemical composition (%) Neutral sugar 24.13 ± 0.04 36.27 ± 3.38 Protein 0.55 ± 0.05 3.16 ± 0.13

* The content ratio (%) of the chemical component represents the ratio in the dry sample.

As shown in Table 1, the yield of the cordless hot water extract prepared in Example 1 was 46.63%, the protein content was 0.55% by weight, and the neutral sugar content was found to be 24.13% by weight. In contrast, the yield of the cordless polymer fraction prepared in Example 2 was low as 1.85%, but the content of neutral sugar was found to be high as 36.27% by weight. As a result, most of the cordless polymer fraction (HEPL) of Example 2 was analyzed by sugar.

Experimental Example 2 Analysis of Enteric Bacterial Proliferation Activity of Hot Water Extract of Example 1 and Polymer Fraction of Example 2

1) Prebiotic acticity assay

Isolation and Identification of Strains for Measuring Intestinal Bacterial Activity

Intestinal beneficial bacteria were obtained for the analysis of the prebiotic activity score of the cordless hot water extract of Example 1 and the cordless polymer fraction of Example 2. These were selected as known enteric beneficial bacteria, and enteric beneficial bacteria were used separately from kimchi, a traditional food.

First, kimchi prepared by suspending in 0.85% physiological saline prepared by the traditional formula to prepare a kimchi suspension. A portion of the suspension was plated in MRS solid medium and incubated at 37 ° C., after completion of the culture, colonies formed in the medium were taken out and cultured in MRS solid medium to obtain pure single colonies uncontaminated. Sequencing analysis was performed by separating 16S rDNA (SEQ ID NO: 1) from each of the obtained strains. As a result, the 16s rDNA sequence was 99% homologous to the existing Lactobacillus paracasei subsp.tolerans (Lactobacillus paracasei subsp. Tolerans) and 99% with the Lactobacillus lactis NCDO604 (T) strain. The strain showing homology of was confirmed. That is, the strains belonging to Lactobacillus paracasei, Lactobacillus plantarum, and Lactococcus lactis NCDO604 (T) (Lactococcus lactis subsp. Lactis) were isolated and identified from kimchi.

The strains were cultured using MRS medium, wherein the culture conditions were pH 6.5 W 0.2, temperature 37 ° C. and stationary incubation for 48 hours, and oxygen demand was permeable anaerobic and preserved via freeze-drying preservation or cell suspension freezing. .

Intestinal beneficial bacteria were Bifidobacterium longum, Bifidobacterium bifidum, Bifidobacterium breve and Escherichia coli KCTC 2441. It was used by the resource center.

Prebiotic acticity measurement

To determine the prebiotic activity according to the extract and the cordless polymer fraction of the previously prepared strains. At this time, the isolated and identified strains were cultured using the following medium: Lactobacillus was used as Lactobacilli MRS solid medium and Lactobacilli MRS broth, and Bifidobacterium was BL solid medium and BL liquid medium (broth). E. coli was used as tryptic-soy agar (TSA) and tryptic-soy broth (TSB). The media of these strains were all purchased from Difco.

In order to measure the prebiotic activity of lactic acid bacteria, Bifidobacterium, Escherichia coli of the extracts or cordless polymer fractions according to the present invention, the present culture is carried out, wherein the culture is M9 MB (minimal medium cell) Using M9 culture medium, glucose (glucose) 2 g / L, CaCl ₂ 0.015 g / L, MgSO ₄ 0.5 g / L was added to use.

Specifically, the cultivation is a colony of each strain in a solid medium (agar) corresponding to each strain, and incubated in the primary culture for 24 hours in a 37 ℃ incubator, then inoculated in 10 ml of broth again 37 Secondary culture was carried out in an incubator for 24 hours. The absorbance (600 nm) of the culture was adjusted to 2.0. The adjusted culture medium was 10 mg / ml concentration of FOS (positive control), glucose (control) and 10 mg / ml concentration of hot hydrothermal extract (Experimental Group 1) or 10 mg / ml concentration of serum-free polymer fraction (Experimental Group 2). 1% (v / v) was inoculated in M9 medium further contained. Immediately after inoculation (0 hours) and 24 hours later, absorbance was measured at 600 nm using a microplate reader, and these values were substituted in Equation 1 below to obtain a prebiotic activity score.

FOS means fructooligosaccharides (FOS).

As a control, one inoculated with 1% (v / v) of bacteria in M9 medium containing 10 mg / ml glucose was used.

[Equation 1]

Where

*) Probiotic log OD on the prebiotic at 24 h was measured 24 hours after inoculating 1% (v / v) of Lactobacillus and Bifidobacterium bacteria in M9 medium containing positive control or experimental groups 1 and 2. One is absorbance.

*) probiotic log O.D. on the prebiotic at 0 h is the absorbance measured after 0 hours of inoculation with 1% (v / v) of Lactobacillus and Bifidobacterium bacteria in M9 medium containing the positive control or experimental groups 1 and 2.

*) probiotic log O.D. on glucose at 24h is the absorbance measured 24 hours after 1% (v / v) inoculation of Lactobacillus and Bifidobacterium bacteria in M9 medium (control) containing 10 mg / ml glucose.

*) probiotic log O.D. on glucose at 0h is the absorbance measured after 0 hours of inoculation of 1% (v / v) of Lactobacillus and Bifidobacterium bacteria in M9 medium (control) containing 10 mg / ml glucose.

*) Enteric log O.D on the prebiotic at 24 h is the absorbance measured 24 hours after 1% (v / v) inoculation of E. coli in M9 medium containing positive control or experimental groups 1 and 2.

*) enteric log O.D. on the prebiotic at 0 h is the absorbance measured after 0 hours of inoculation of 1% (v / v) of E. coli in M9 medium containing the positive control group or experimental groups 1 and 2.

*) enteric log O.D. on glucose at 24h is the absorbance measured 24 hours after 1% (v / v) inoculation of E. coli in M9 medium (control) containing 10 mg / ml glucose.

*) enteric log O.D. on glucose at 0h is the absorbance measured after 0 hours of inoculation of 1% (v / v) of E. coli in M9 medium (control) containing 10 mg / ml glucose.

2) Experiment result

Prebiotic activity score on radish hot water extract.

Figure 1 shows the prebiotic activity score when the various bacteria were treated in M9 medium containing the radish hot water extract (10 mg / ㎖), FOS (fructo oligosaccharides) (10 mg / ㎖) of Example 1 This graph shows the analysis. 1A is for Lactobacillus paracasei subsp.tolerans (Lactobacillus paracasei subsp. Tolerans), FIG. 1B is Lactobacillus plantarum, FIG. 1C is Lactobacillus lactis NCDO604 (T) ( Lactococcus lactis subsp.lactis, FIG. 1D for Bifidobacterium longum, FIG. 1E for Bifidobacterium bifidum, FIG. 1F for Bifidobacterium breve.

Figure 3A is a graph showing the analysis of the prebiotic activity score (prebiotic activity score) for the total bacteria of the cordless hydrothermal extract, FOS (fructo oligosaccharide) of Example 1.

As shown in Figure 1, each strain was administered the conventional prebiotic fructo oligosaccharide and the radish hot water extract of Example 1, and confirmed the prebiotic activity score. Referring to Figure 1, the radish hot water extract of Example 1 according to the present invention was significantly superior to the prebiotic activity for all bacteria, compared to the control group was not treated anything, it was confirmed that the activity against E. coli.

In addition, compared to the conventional prebiotic fructo oligosaccharides, the radish hot water extract of the present invention was found to have almost the same level of prebiotic activity against Lactobacillus strain, Lactococcus strain and Bifidobacteria strain. Specifically, Lactobacillus paracasei had higher 1.032 prebiotic activity than fructo oligosaccharide (0.939), which is widely known as a conventional prebiotic.

All of the radish hot water extracts of the present invention were found to have high activity compared to the control group, and most of them were found to have the same level of activity as compared to the FOS, the positive control group. It confirmed that it was high.

As shown in FIG. 3A, as a result of comparing the prebiotic activity of the overall enteric beneficial bacteria, the radish hot water extract was 0.764, which was somewhat lower than that of the FOS group, but for beneficial bacteria such as lactobacillus, Lactococcus, and Bacillus, which are anaerobic strains. It can be seen that the ability to increase prebiotic activity remains on par with that of FOS. That is, it can be seen that the whole intestinal beneficial bacteria have more than three times more prebiotic activity than the control group.In particular, for the beneficial bacteria such as lactobacillus and lactococcus, which are the anaerobic strains, the prebiotic activity is equivalent to that of the FOS group or It can be seen that it exhibits significantly superior activity beyond that. Through this, the radish extract of the present invention has the effect of promoting the growth of enteric beneficial bacteria, inhibit the growth of enteric harmful bacteria to maintain the balance between enteric beneficial bacteria and harmful bacteria (E. coli).

In addition, the extract of the present invention is prominent in the proliferative effect of the intestinal anaerobic strains such as lactobacillus, lactococcus and the like compared to the conventional representative fructooligosaccharide, probiotics composition for activating the proliferation of intestinal beneficial bacteria and for improving gut health or intestinal function It can be seen that it can be used as a composition.

Intestinal Beneficial Activity Score Analysis for the Molecular Polymer Fractions

FIG. 2 shows the prebiotic activity score of various microorganisms in M9 medium containing the cordless polymer fraction of Example 2 (10 mg / ml) and FOS (fructo oligosaccharides) (10 mg / ml). This graph shows the analysis. 2A is for Lactobacillus paracasei subsp.tolerans (Lactobacillus paracasei subsp. Tolerans), FIG. 2B is Lactobacillus plantarum, FIG. 2C is Lactobacillus lactis NCDO604 (T) ( Lactococcus lactis subsp.lactis, FIG. 2D for Bifidobacterium longum, FIG. 2E for Bifidobacterium bifidum, FIG. 2F for Bifidobacterium breve It is about.

Figure 3B is a graph showing the analysis of the intestinal beneficial bacteria activity (prebiotic activity score) for the total bacteria of the cordless polymer fraction, FOS (fructo oligosaccharide) of Example 2.

Each strain was inoculated and cultured in M9 medium containing the conventional prebiotic fructo oligosaccharide and the cordless polymer fraction of Example 2 to confirm the prebiotic activity score. As shown in Figure 2, it was confirmed that the activity of the majority of the intestinal beneficial bacteria of the cordless polymer fraction of Example 2 according to the present invention has significantly superior activity than the control (about 2 to 6 times).

In addition, compared to the conventional prebiotic fructo oligosaccharides, the cordless polymer fraction of the present invention was found to exhibit a significantly superior level of prebiotic activity except for Bifidobacterium longgum and Bifidobacterium bifidum. Specifically, it was confirmed that the cordless polymer fraction of the present invention had about 1.5 times higher prebiotic activity against Lactobacillus paracasei, Lactobacillus plantarum, and Lactococcus lactis compared to the radish hot water extract. This is about 1.5 times higher than the radish hot water extract of the present invention.

Taken together, the cordless polymer fraction of the present invention was found to have a remarkably excellent prebiotic activity against the majority of the beneficial bacteria compared to the control group, even showing a level of prebiotic activity equivalent or remarkably superior to the most widely used fructo oligosaccharides. It was confirmed.

In particular, the cordless polymer fraction of the present invention exhibits about 1.5 times higher prebiotic activity with respect to Lactobacillus paracasei, Lactobacillus plantarum, and Lactococcus lactis, compared to the radish hot water extract. As a result of comparing the prebiotic activity, the cordless polymer fraction was 0.894, which is lower than that of the FOS group, but increased compared to the control (0.202) and the radish hot water extract (0.764).

In summary, both the hot water extract and the cordless polymer fraction were significantly higher in the prebiotic activity level of enteric beneficial bacteria than the control. Especially, the radish hot water extract had the same ability to increase prebiotic activity against beneficial bacteria such as Lactobacillus, Lactococcus, Bifidobacteria and so on. It was confirmed that the beneficial bacteria such as lactococcus had superior activity compared to FOS and radish hot water extract.

The polymer hydrothermal extract is a hydrothermal extract that has not undergone a separate process, and contains a large amount of low molecular water-soluble components that are not included in the cordless polymer fraction. After all, the low molecular weight material of less than 10 kDa of the polymer hydrothermal extract is believed to have a significant increase in the prebiotic activity of beneficial bacteria such as anaerobic strains such as fibidobacteria.

In addition, unlike the cordless hot water extract, the cordless polymer fraction was removed from the macromolecular substance of less than 10 kDa by using a dialysis membrane, so that the overall prebiotic activity was increased to the same or significantly superior level to the FOS (FIG. 2). It was confirmed that the beneficial bacteria such as Lactobacillus and Lactococcus were more than 1.5 times improved compared to FOS and radish hydrothermal extract. That is, in the case of the radish hot water extract, the polymer fraction and the low molecular material are present in a mixed state, and due to the complex action therebetween, the prebiotic activity of beneficial bacteria such as lactobacillus and lactococcus is low. The tic activity and the prebiotic activity of the anaerobic strain Bifidobacteria are considered to be high.

Therefore, it can be seen that it can be appropriately selected from the hydrothermal extract and the polymer fraction according to the desired purpose or desired effect.

Hereinafter, the preparation examples of the composition containing the powder of the present invention will be described, but the present invention is not intended to limit the present invention but only to explain in detail.

Formulation Example 1 Preparation of Powder

Radish hot water extract obtained in Example 1; or

The cordless polymer fraction obtained in Example 2; 500 mg

Lactose 100 mg

Talc 10 mg

The above ingredients are mixed and filled in an airtight cloth to prepare a powder.

Formulation Example 2 Preparation of Tablet

Radish hot water extract obtained in Example 1; or

The cordless polymer fraction obtained in Example 2; 300 mg

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

After mixing the above components, tablets are prepared by tableting according to a conventional method for preparing tablets.

Formulation Example 3 Preparation of Capsule

Radish hot water extract obtained in Example 1; or

The cordless polymer fraction obtained in Example 2; 200 mg

3 mg of crystalline cellulose

Lactose 14.8 mg

Magnesium Stearate 0.2 mg

According to a conventional capsule preparation method, the above ingredients are mixed and filled into gelatin capsules to prepare capsules.

Formulation Example 4 Preparation of Injection

Radish hot water extract obtained in Example 1; or

The cordless polymer fraction obtained in Example 2; 600 mg

Mannitol 180 mg

Sterile distilled water for injection 2974 mg

Na ₂ HPO _4, 12H ₂ O 26 mg

According to the conventional method for preparing an injection, the amount of the above ingredient is prepared per ampoule.

Formulation Example 5 Preparation of Liquid

Radish hot water extract obtained in Example 1; or

The cordless polymer fraction obtained in Example 2; 4 g

10 g of isomerized sugar

5 g of mannitol

Purified water

After dissolving each component in purified water according to the usual method of preparing a liquid solution, adding lemon flavor, and then mixing the above components, and adding purified water to adjust the total to 100g by adding purified water and filling it into a brown bottle to sterilize To prepare a liquid solution.

Formulation Example 6 Preparation of Granules

Radish hot water extract obtained in Example 1; or

The cordless polymer fraction obtained in Example 2; 1,000 mg

Vitamin mixture proper amount

70 μg of Vitamin A Acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

Vitamin B6 0.5 mg

0.2 μg of vitamin B12

Vitamin C 10 mg

10 μg biotin

Nicotinamide 1.7 mg

Folate 50 ㎍

Calcium Tothenate 0.5 mg

Substrate mixture

Ferrous Acid 1.75 mg

Zinc Flower 0.82 mg

Magnesium Acid 25.3 mg

15 mg potassium monophosphate

Calcium Diphosphate 55 mg

Potassium acetate 90 mg

Calcium Acid 100 mg

Magnesium fluoride 24.8 mg

Although the composition ratio of the vitamin and mineral mixture of the group is a relatively suitable composition for the granules in a preferred embodiment, the composition ratio may be arbitrarily modified, and after mixing the above components in accordance with the conventional granulation method, granules, It can be prepared and used in the manufacture of health functional food composition according to a conventional method.

Example 7 Preparation of Functional Beverage

Radish hot water extract obtained in Example 1; or

The cordless polymer fraction obtained in Example 2; 1,000 mg

1,000 mg / year

100 g of sugar

2 g of real concentrate

We 1 g

900 mL total with distillation

After mixing the above components according to the healthy beverage manufacturing method of the phase, and then stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed sterilized and then refrigerated and stored in the present invention In the preparation of functional beverage compositions.

Although the composition ratio is a composition that is relatively suitable for the preferred beverage in a preferred embodiment, the compounding ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and usage.

Claims (8)

delete delete delete delete A prebiotic composition comprising a cordless polymer fraction prepared as an active ingredient by precipitating the cordless hot water extract with alcohol and recovering only the polymer fraction having a molecular weight of 10 kDa or more using ultrafiltration or gel filtration chromatography.
The prebiotic composition has a prebiotic activity that relatively proliferates enteric beneficial bacteria compared to enteric harmful bacteria,
The enteric benefit bacterium is any one or more selected from the group consisting of Lactobacillus paracasei, Lactobacillus plantarum, Lactococcus lactis, Bifidobacterium longgum, Bifidobacterium bifidem, and Bifidobacterium brave ,
The enteric harmful bacterium is E. coli prebiotic composition, characterized in that. delete (a) precipitating the cordless hot water extract to alcohol; And
(b) recovering only the polymer fraction having a molecular weight of 10 kDa or more using ultrafiltration or gel filtration chromatography. The method of claim 7, wherein
The (a) radish extract is a method of producing a cordless polymer fraction, characterized in that it was prepared through (a-1) hot water extraction for 2 to 5 hours at 70 to 100 ℃.

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