KR20230163393A - 3-메틸크로톤산 데카복실라제(mdc) 변이체 - Google Patents

3-메틸크로톤산 데카복실라제(mdc) 변이체 Download PDF

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Publication number
KR20230163393A
KR20230163393A KR1020237032469A KR20237032469A KR20230163393A KR 20230163393 A KR20230163393 A KR 20230163393A KR 1020237032469 A KR1020237032469 A KR 1020237032469A KR 20237032469 A KR20237032469 A KR 20237032469A KR 20230163393 A KR20230163393 A KR 20230163393A
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KR
South Korea
Prior art keywords
amino acid
seq
deleted
sequence shown
substituted
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
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KR1020237032469A
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English (en)
Korean (ko)
Inventor
베누아 빌리에
프랑수아 스트리처
Original Assignee
글로벌 바이오에너지스
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Publication date
Application filed by 글로벌 바이오에너지스 filed Critical 글로벌 바이오에너지스
Publication of KR20230163393A publication Critical patent/KR20230163393A/ko
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01035—UDP-glucuronate decarboxylase (4.1.1.35), i.e. UDP-D-xylose synthase

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
KR1020237032469A 2021-03-31 2022-03-30 3-메틸크로톤산 데카복실라제(mdc) 변이체 Pending KR20230163393A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP21166368.7 2021-03-31
EP21166368 2021-03-31
PCT/EP2022/058372 WO2022207684A1 (en) 2021-03-31 2022-03-30 3-methylcrotonic acid decarboxylase (mdc) variants

Publications (1)

Publication Number Publication Date
KR20230163393A true KR20230163393A (ko) 2023-11-30

Family

ID=75339587

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020237032469A Pending KR20230163393A (ko) 2021-03-31 2022-03-30 3-메틸크로톤산 데카복실라제(mdc) 변이체

Country Status (10)

Country Link
US (1) US20250320482A1 (https=)
EP (1) EP4314269A1 (https=)
JP (1) JP2024511641A (https=)
KR (1) KR20230163393A (https=)
CN (1) CN117120604A (https=)
AU (1) AU2022248562A1 (https=)
BR (1) BR112023019721A2 (https=)
CA (1) CA3210264A1 (https=)
IL (1) IL307259A (https=)
WO (1) WO2022207684A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2026003122A1 (en) 2024-06-26 2026-01-02 Global Bioenergies Method for producing isobutene from acetic acid

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2433260A (en) 2005-12-16 2007-06-20 Mologic Ltd A selectable decarboxylase marker
WO2010001078A2 (fr) 2008-07-04 2010-01-07 Philippe Marliere Production d'alcenes par decarboxylation enzymatique d'acides 3-hydroxy-alcanoïques
US10988752B2 (en) * 2016-05-04 2021-04-27 Global Bioenergies 3-methylcrotonic acid decarboxylase (MDC) variants
MA44868A (fr) 2016-05-04 2019-03-13 Global Bioenergies Variantes de la décarboxylase de l'acide 3-méthylcrotonique (mdc)
WO2020007886A1 (en) 2018-07-03 2020-01-09 Global Bioenergies 3-methylcrotonic acid decarboxylase (mdc) variants

Also Published As

Publication number Publication date
AU2022248562A1 (en) 2023-09-07
JP2024511641A (ja) 2024-03-14
US20250320482A1 (en) 2025-10-16
CA3210264A1 (en) 2022-10-06
BR112023019721A2 (pt) 2023-10-31
WO2022207684A1 (en) 2022-10-06
EP4314269A1 (en) 2024-02-07
IL307259A (en) 2023-11-01
CN117120604A (zh) 2023-11-24

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Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20230921

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20250131

Comment text: Request for Examination of Application