CN117120604A - 3-甲基巴豆酸脱羧酶(mdc)变体 - Google Patents

3-甲基巴豆酸脱羧酶(mdc)变体 Download PDF

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Publication number
CN117120604A
CN117120604A CN202280024402.8A CN202280024402A CN117120604A CN 117120604 A CN117120604 A CN 117120604A CN 202280024402 A CN202280024402 A CN 202280024402A CN 117120604 A CN117120604 A CN 117120604A
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CN
China
Prior art keywords
amino acid
seq
deleted
position corresponding
acid residue
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202280024402.8A
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English (en)
Chinese (zh)
Inventor
B·维利耶
F·斯特里彻尔
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Global Bioenergies SA
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Global Bioenergies SA
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Publication of CN117120604A publication Critical patent/CN117120604A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01035—UDP-glucuronate decarboxylase (4.1.1.35), i.e. UDP-D-xylose synthase

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
CN202280024402.8A 2021-03-31 2022-03-30 3-甲基巴豆酸脱羧酶(mdc)变体 Pending CN117120604A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP21166368.7 2021-03-31
EP21166368 2021-03-31
PCT/EP2022/058372 WO2022207684A1 (en) 2021-03-31 2022-03-30 3-methylcrotonic acid decarboxylase (mdc) variants

Publications (1)

Publication Number Publication Date
CN117120604A true CN117120604A (zh) 2023-11-24

Family

ID=75339587

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202280024402.8A Pending CN117120604A (zh) 2021-03-31 2022-03-30 3-甲基巴豆酸脱羧酶(mdc)变体

Country Status (10)

Country Link
US (1) US20250320482A1 (https=)
EP (1) EP4314269A1 (https=)
JP (1) JP2024511641A (https=)
KR (1) KR20230163393A (https=)
CN (1) CN117120604A (https=)
AU (1) AU2022248562A1 (https=)
BR (1) BR112023019721A2 (https=)
CA (1) CA3210264A1 (https=)
IL (1) IL307259A (https=)
WO (1) WO2022207684A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2026003122A1 (en) 2024-06-26 2026-01-02 Global Bioenergies Method for producing isobutene from acetic acid

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2433260A (en) 2005-12-16 2007-06-20 Mologic Ltd A selectable decarboxylase marker
WO2010001078A2 (fr) 2008-07-04 2010-01-07 Philippe Marliere Production d'alcenes par decarboxylation enzymatique d'acides 3-hydroxy-alcanoïques
US10988752B2 (en) * 2016-05-04 2021-04-27 Global Bioenergies 3-methylcrotonic acid decarboxylase (MDC) variants
MA44868A (fr) 2016-05-04 2019-03-13 Global Bioenergies Variantes de la décarboxylase de l'acide 3-méthylcrotonique (mdc)
WO2020007886A1 (en) 2018-07-03 2020-01-09 Global Bioenergies 3-methylcrotonic acid decarboxylase (mdc) variants

Also Published As

Publication number Publication date
AU2022248562A1 (en) 2023-09-07
KR20230163393A (ko) 2023-11-30
JP2024511641A (ja) 2024-03-14
US20250320482A1 (en) 2025-10-16
CA3210264A1 (en) 2022-10-06
BR112023019721A2 (pt) 2023-10-31
WO2022207684A1 (en) 2022-10-06
EP4314269A1 (en) 2024-02-07
IL307259A (en) 2023-11-01

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