KR20230140351A - Method for manufacturing plant biostimulant for enhancing phyto-nutrients and composition thereof - Google Patents
Method for manufacturing plant biostimulant for enhancing phyto-nutrients and composition thereof Download PDFInfo
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- 238000004519 manufacturing process Methods 0.000 title claims description 15
- 238000000034 method Methods 0.000 title claims description 12
- 230000002708 enhancing effect Effects 0.000 title description 2
- 239000000203 mixture Substances 0.000 title description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 34
- OENHQHLEOONYIE-UKMVMLAPSA-N all-trans beta-carotene Natural products CC=1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C OENHQHLEOONYIE-UKMVMLAPSA-N 0.000 claims abstract description 16
- 235000013734 beta-carotene Nutrition 0.000 claims abstract description 16
- TUPZEYHYWIEDIH-WAIFQNFQSA-N beta-carotene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2=CCCCC2(C)C TUPZEYHYWIEDIH-WAIFQNFQSA-N 0.000 claims abstract description 16
- 239000011648 beta-carotene Substances 0.000 claims abstract description 16
- 229960002747 betacarotene Drugs 0.000 claims abstract description 16
- OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 claims abstract description 16
- 241001131796 Botaurus stellaris Species 0.000 claims abstract description 12
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 12
- 150000003839 salts Chemical class 0.000 claims abstract description 12
- 239000013535 sea water Substances 0.000 claims abstract description 9
- 238000010612 desalination reaction Methods 0.000 claims abstract description 7
- 241000894006 Bacteria Species 0.000 claims abstract description 6
- 239000004310 lactic acid Substances 0.000 claims abstract description 6
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 6
- 239000012190 activator Substances 0.000 claims abstract description 5
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims abstract description 4
- 241000196324 Embryophyta Species 0.000 claims description 67
- 239000013543 active substance Substances 0.000 claims description 26
- 239000012867 bioactive agent Substances 0.000 claims description 18
- 235000009337 Spinacia oleracea Nutrition 0.000 claims description 12
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 11
- 229910052799 carbon Inorganic materials 0.000 claims description 11
- 235000002566 Capsicum Nutrition 0.000 claims description 9
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 9
- 239000011707 mineral Substances 0.000 claims description 9
- 241000758706 Piperaceae Species 0.000 claims description 8
- 244000005700 microbiome Species 0.000 claims description 8
- 244000000626 Daucus carota Species 0.000 claims description 5
- 235000002767 Daucus carota Nutrition 0.000 claims description 5
- 238000007738 vacuum evaporation Methods 0.000 claims description 5
- 240000002234 Allium sativum Species 0.000 claims description 4
- 235000004611 garlic Nutrition 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 238000000855 fermentation Methods 0.000 claims description 3
- 230000004151 fermentation Effects 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 238000005086 pumping Methods 0.000 claims description 3
- 244000300264 Spinacia oleracea Species 0.000 claims 1
- 238000011081 inoculation Methods 0.000 claims 1
- 230000001965 increasing effect Effects 0.000 abstract description 5
- 235000012041 food component Nutrition 0.000 abstract description 2
- 241000219315 Spinacia Species 0.000 description 11
- 238000004458 analytical method Methods 0.000 description 9
- 238000000746 purification Methods 0.000 description 8
- 230000003078 antioxidant effect Effects 0.000 description 7
- 239000003963 antioxidant agent Substances 0.000 description 5
- 235000006708 antioxidants Nutrition 0.000 description 5
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 5
- 235000015097 nutrients Nutrition 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 240000008415 Lactuca sativa Species 0.000 description 3
- 235000003228 Lactuca sativa Nutrition 0.000 description 3
- 235000004347 Perilla Nutrition 0.000 description 3
- 244000124853 Perilla frutescens Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000003556 assay Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000008213 purified water Substances 0.000 description 2
- WMBWREPUVVBILR-WIYYLYMNSA-N (-)-Epigallocatechin-3-o-gallate Chemical compound O([C@@H]1CC2=C(O)C=C(C=C2O[C@@H]1C=1C=C(O)C(O)=C(O)C=1)O)C(=O)C1=CC(O)=C(O)C(O)=C1 WMBWREPUVVBILR-WIYYLYMNSA-N 0.000 description 1
- JDLKFOPOAOFWQN-VIFPVBQESA-N Allicin Natural products C=CCS[S@](=O)CC=C JDLKFOPOAOFWQN-VIFPVBQESA-N 0.000 description 1
- 241001474374 Blennius Species 0.000 description 1
- WMBWREPUVVBILR-UHFFFAOYSA-N GCG Natural products C=1C(O)=C(O)C(O)=CC=1C1OC2=CC(O)=CC(O)=C2CC1OC(=O)C1=CC(O)=C(O)C(O)=C1 WMBWREPUVVBILR-UHFFFAOYSA-N 0.000 description 1
- 239000006002 Pepper Substances 0.000 description 1
- 235000016761 Piper aduncum Nutrition 0.000 description 1
- 235000017804 Piper guineense Nutrition 0.000 description 1
- 244000203593 Piper nigrum Species 0.000 description 1
- 235000008184 Piper nigrum Nutrition 0.000 description 1
- JDLKFOPOAOFWQN-UHFFFAOYSA-N allicin Chemical compound C=CCSS(=O)CC=C JDLKFOPOAOFWQN-UHFFFAOYSA-N 0.000 description 1
- 235000010081 allicin Nutrition 0.000 description 1
- 239000012267 brine Substances 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 239000011785 micronutrient Substances 0.000 description 1
- 235000013369 micronutrients Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
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- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
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Abstract
본 발명의 목적은 식물의 영양 성분, 특히, 베타카로틴 성분을 현저히 증간 시키기 위한 바이오스티뮬런트를 제공하고자 하는 것이다.
상기 목적에 따라 본 발명은 해양심층수, 해수담수화 설비, 또는 발전소에서 발생하는 간수(Bittern, Nigari)에 대해 내염성 유산균으로 발효 시킨 바이오스티뮬런트(식물생리활성제)를 제공한다.The purpose of the present invention is to provide a biostimulant for significantly increasing the nutritional components of plants, especially beta-carotene components.
In accordance with the above purpose, the present invention provides a biostimulant (plant biological activator) fermented with salt-resistant lactic acid bacteria for bittern (Nigari) generated from deep ocean water, seawater desalination facilities, or power plants.
Description
본 발명은 식물 영양성분 증강용 식물 바이오스티뮬런트의 제조 방법 및 그 조성물에 관한 것이다.The present invention relates to a method for producing a plant biostimulant for enhancing plant nutrients and a composition thereof.
식물의 특정 영양성분을 증강시킬 수 있는 식물 바이오스티뮬런트는 임의의 물질군·미생물을 함유하고, 식물체 내에서도 일어나고 있는 일련의 프로세스를 자극함하여 양분 흡수를 향상시키거나, 시비 효율을 높이거나, 스트레스 내성을 부여하고, 품질을 향상시킬 수 있는 것으로, 살충·살균제로 분류되지 않는 것을 말한다. 자연계에 존재하는 물질군·미생물 포함 물질로, 식물호르몬이나 영양분은 아니지만, 극히 소량으로도 식물의 활력을 자극하고, 생육을 촉진하는 물질이다. Plant biostimulants, which can enhance specific nutrients in plants, contain a certain group of substances and microorganisms and stimulate a series of processes occurring within the plant to improve nutrient absorption, increase fertilization efficiency, or reduce stress. It refers to something that can confer resistance and improve quality, and is not classified as an insecticide or disinfectant. It is a group of substances and microorganisms that exist in the natural world, and although it is not a plant hormone or nutrient, it is a substance that stimulates the vitality of plants and promotes growth even in extremely small amounts.
공개특허 10-2021-0151932호는 셀로올리고당을 포함하는 바이오스티뮬런트를 제안하여, 농작물의 활력, 수량, 품질 및 수확 후의 보존성을 개선한다. 그러나 그외, 식물의 영양성분 증강 효과를 증강시킨 것은 아니다.Publication Patent No. 10-2021-0151932 proposes a biostimulant containing cellooligosaccharides to improve crop vitality, yield, quality, and post-harvest preservation. However, other than that, it does not enhance the nutritional enhancement effect of plants.
따라서 본 발명의 목적은 식물의 영양 성분, 특히, 베타카로틴 성분을 현저히 증간 시키기 위한 바이오스티뮬런트를 제공하고자 하는 것이다.Therefore, the purpose of the present invention is to provide a biostimulant for significantly increasing the nutritional components of plants, especially beta-carotene components.
상기 목적에 따라 본 발명은 해양심층수, 해수담수화 설비, 또는 발전소에서 발생하는 간수(Bittern, Nigari)에 대해 내염성 유산균으로 발효 시킨 바이오스티뮬런트(식물생리활성제)를 제공한다.In accordance with the above purpose, the present invention provides a biostimulant (plant biological activator) fermented with salt-resistant lactic acid bacteria for bittern (Nigari) generated from deep ocean water, seawater desalination facilities, or power plants.
즉, 본 발명은,That is, the present invention:
해양심층수, 해양 심층수, 해수담수화 설비 또는 화력발전소에서 얻은 간수(Bittern, Nigari)를 카본 필터로 정제하고,Deep ocean water, deep ocean water, bittern (Bittern, Nigari) obtained from seawater desalination plants or thermal power plants is purified using a carbon filter,
염분을 제거하고,remove salt,
내염성 유산균을 접종하여 발효하여,Fermented by inoculating salt-tolerant lactic acid bacteria,
식물의 베타카로틴 성분 함량을 증강시키는 식물생리활성제의 제조방법을 제공한다.Provided is a method for producing a plant biological activator that enhances the beta-carotene content of plants.
상기에 있어서, 상기 카본 필터는, 다수의 카본 블록이 다층으로 배열되고, 해양심층수, 해양 심층수, 해수담수화 설비 또는 화력발전소에서 얻은 간수는 아래층에서 입수되어 펌핑 작용에 의해 위층을 통과하여 배출되면서 정체되는 것을 특징으로 하는 식물생리활성제의 제조방법을 제공한다.In the above, the carbon filter has a plurality of carbon blocks arranged in multiple layers, and deep ocean water, deep ocean water, bittern water obtained from a seawater desalination plant or thermal power plant is collected from the lower layer, passes through the upper layer through a pumping action, and is discharged, thereby stagnating. A method for producing a plant biological active agent is provided.
상기에 있어서, 상기 카본 블록은 내부에 자외선 램프를 포함한 것을 특징으로 하는 식물생리활성제의 제조방법을 제공한다.In the above, a method for producing a plant biological active agent is provided, wherein the carbon block includes an ultraviolet lamp therein.
상기에 있어서, 염분의 제거는 진공 증발원에 넣어 염분이 제거된 증발된 수증기를 응결 시켜 미네랄 농축수로 수집하여 이루어진 것을 특징으로 하는 식물생리활성제의 제조방법을 제공한다.In the above, a method for producing a plant biological active agent is provided, wherein the salt is removed by putting it in a vacuum evaporation source, condensing the evaporated water vapor from which the salt has been removed, and collecting it as mineral concentrated water.
상기에 있어서, 미네랄 농축수 10 내지 30 중량부, 펩톤 0 내지 5 중량부, 포도당 5 내지 20 중량부 및 증류수 10 내지 80 중량부의 배지를 제조하고, 상기 배지에 내염성 미생물 1~10 중량부를 접종하여 25 내지 35℃에서 72~120시간 동안 발효시키는 과정을 거쳐 식물생리활성제가 제조되는 것을 특징으로 하는 식물생리활성제의 제조방법을 제공한다.In the above, a medium containing 10 to 30 parts by weight of mineral concentrated water, 0 to 5 parts by weight of peptone, 5 to 20 parts by weight of glucose, and 10 to 80 parts by weight of distilled water was prepared, and 1 to 10 parts by weight of salt-tolerant microorganisms were inoculated into the medium. A method for producing a plant biological active agent is provided, wherein the plant biological active agent is produced through fermentation at 25 to 35° C. for 72 to 120 hours.
상기 제조방법으로 제조된 식물생리활성제를 제공한다.A plant biological active agent prepared by the above production method is provided.
상기의 식물생리활성제를 250ppm 내지 500ppm 농도로 하여 식물재배에 처리하는 것을 특징으로 하는 식물생리활성제 처리 방법을 제공한다.A method of treating plant biological active agents is provided, characterized in that the plant biological active agent is applied to plant cultivation at a concentration of 250 ppm to 500 ppm.
상기에 있어서, 상기 시물생리활성제의 처리는 시금치, 고추, 당근 또는 마늘에 대해 이루어져 베타카로틴의 함량을 증폭시키는 것을 특징으로 하는 식물생리활성제 처리 방법을 제공한다.In the above, the treatment with the plant bioactive agent is performed on spinach, peppers, carrots, or garlic to provide a plant bioactive agent treatment method, characterized in that the content of beta-carotene is amplified.
본 발명에 따른 바이오스티뮬런트를 적용하여 성장시킨 시금치의 경우, 베타카로틴 함량이 100g 당 222,946μg 을 함유하여 일반적인 시금치에 비해 77배 정도의 고함량을 보였다. In the case of spinach grown by applying the biostimulant according to the present invention, the beta-carotene content was 222,946 μg per 100 g, which was about 77 times higher than that of general spinach.
도 1은 본 발명의 식물생리활성제의 제조방법을 개략적으로 설명하는 순서도이다.
도 2는 식물생리활성제에 대해 항산화 평가 (DPPH assay)를 실시한 결과를 나타낸 그래프이다.
도 3은 본 발명의 식물생리활성제를 투여하여 재배한 시금치 외관을 보여주는 사진이다.
도 4는 본 발명의 식물생리활성제를 투여하여 재배한 시금치, 당근, 깻잎의 외관과 베타카로틴 함량을 기재한 사진이다.
도 5는 본 발명의 식물생리활성제를 투여하여 재배한 시금치의 영양성분 분석 표이다.
도 6은 본 발명의 식물생리활성제를 투여하여 재배한 말린 고추의 외관과 수확량 등의 비교군과 대비를 보여주는 표이다.
도 7은 본 발명의 식물생리활성제를 투여하여 재배한 마늘의 외관과 성분 분석 결과를 보여주는 도면이다.
도 8은 본 발명의 식물생리활성제를 투여하여 재배한 상추의 외관과 분석결과를 보여주는 사진이다.
도 9는 본 발명의 식물생리활성제를 투여하여 재배한 생고추의 외관과 분석 결과를 보여주는 사진이다.1 is a flowchart schematically explaining the method for producing the plant biological active agent of the present invention.
Figure 2 is a graph showing the results of antioxidant evaluation (DPPH assay) on plant biological active agents.
Figure 3 is a photograph showing the appearance of spinach grown by administering the plant bioactive agent of the present invention.
Figure 4 is a photograph depicting the appearance and beta-carotene content of spinach, carrots, and perilla leaves grown by administering the plant bioactive agent of the present invention.
Figure 5 is a nutrient analysis table of spinach grown by administering the plant bioactive agent of the present invention.
Figure 6 is a table showing the comparison group and comparison of the appearance and yield of dried peppers grown by administering the plant biological active agent of the present invention.
Figure 7 is a diagram showing the appearance and component analysis results of garlic grown by administering the plant biological active agent of the present invention.
Figure 8 is a photograph showing the appearance and analysis results of lettuce grown by administering the plant biological active agent of the present invention.
Figure 9 is a photograph showing the appearance and analysis results of raw peppers grown by administering the plant biological active agent of the present invention.
이하, 첨부도면을 참조하여 본 발명의 바람직한 실시예에 대해 상세히 설명한다.Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.
도 1은 본 발명의 바이오스티뮬런트의 제조방법을 개략적으로 설명하는 순서도이다. 1 is a flow chart schematically explaining the method for producing the biostimulant of the present invention.
최상의 미네랄을 풍부하게 함유하지만 염분이 높은 해양 심층수 또는 간수를 채취하여 정제하고, 염분을 제거한 후, 내염성 유산균(super microbiome)으로 발효시켜 항산화 물질과 미량 영양소가 풍부한 바이오스티뮬런트(식물생리활성제)를 제조한다.Biostimulant (plant biological activator) rich in antioxidants and micronutrients made by collecting and purifying deep ocean water or brackish water, which is rich in the best minerals but with high salinity, removing the salt, and fermenting it with salt-tolerant lactic acid bacteria (super microbiome). manufactures.
정제 단계는 정제 필터를 이용하여 해양 심층수, 해수담수화 설비 또는 화력발전소에서 얻은 간수로 하여금 정제 필터를 거치게 한다. 정제 필터는 다층의 카본 블록과 자외선 살균기를 포함한다. 카본 블록 내부에 자외선 램프를 배열한 하나의 단위 필터를 만들고, 이를 연속하여 배열하여 정제 필터를 구성한다. 해양 심층수 또는 간수가 정제 필터를 통과하며, 유해물질과 중금속 등이 제거된다. 단위 필터는 수평으로 다수개 연속 배치되고, 다시 그 위에 적층되어 다층을 이룬다. 해양 심층수 또는 간수는 아래층에 배열된 단위 필터로 진입하여 수평 배열된 다수의 단위 필터를 모두 거친 후 위층에 놓인 단위 필터로 상승하도록 펌핑된다. 모든 층고의 단위 필터를 거친 후 최상층 단위 필터로부터 정제수가 배출된다. 카본 블록 내에 배열되는 자외선 램프는 선형 또는 코일 형태일 수 있으며, 코일 형태로 된 자외선 램프는 출력을 강화할 수 있어 살균력이 강화되어 바람직하다. 펌프의 펌핑 압력을 조절하여 정제수의 정제 시간과 정제 정도를 조절한다.In the purification step, bittern obtained from deep ocean water, seawater desalination facilities, or thermal power plants is passed through a purification filter. The purification filter contains multi-layer carbon blocks and an ultraviolet sterilizer. A unit filter is made by arranging ultraviolet lamps inside a carbon block, and these are arranged in series to form a purification filter. Deep ocean water or bittern water passes through a purification filter, and harmful substances and heavy metals are removed. A plurality of unit filters are arranged horizontally in succession, and then stacked on top of each other to form a multi-layer. Deep ocean water or bittern water enters the unit filters arranged in the lower layer, passes through a plurality of horizontally arranged unit filters, and is then pumped to rise to the unit filters placed in the upper layer. After passing through unit filters at all floor heights, purified water is discharged from the unit filter on the top floor. The ultraviolet lamps arranged within the carbon block may be linear or coiled, and coil-shaped ultraviolet lamps are preferred because they can enhance output and enhance sterilizing power. By adjusting the pumping pressure of the pump, the purification time and degree of purification of purified water are controlled.
정제단계를 거친 해양심층수 또는 간수는 염분 제거를 위해 진공 증발원에서 농축된다. 진공 증발원은 상용화된 것을 사용한다. 진공 증발원에서 증발된 수증기는 염분이 없는 상태이며, 재응결 시켜 미네랄 농축수로 수집하고, 잔류 염분도 별도로 수집한다. Deep sea water or brine that has gone through the purification stage is concentrated in a vacuum evaporation source to remove salts. A commercially available vacuum evaporation source is used. The water vapor evaporated from the vacuum evaporation source is salt-free, and is re-condensed and collected as mineral concentrated water, and residual salts are also collected separately.
수집한 염분 제거 미네랄 농축수는 내염성 미생물을 접종하여 발효 시킨다. The collected salt-removed mineral concentrated water is inoculated with salt-tolerant microorganisms and fermented.
미네랄 농축수 10 내지 30 중량부, 펩톤 0 내지 5 중량부, 포도당 5 내지 20 중량부 및 증류수 10 내지 80 중량부의 배지에 내염성 미생물을 접종하여 발효시킨다.Salt-tolerant microorganisms are inoculated into a medium containing 10 to 30 parts by weight of mineral concentrated water, 0 to 5 parts by weight of peptone, 5 to 20 parts by weight of glucose, and 10 to 80 parts by weight of distilled water and fermented.
내염성 미생물 1~10 중량부를 상기 배지에 접종하여 25 내지 35℃에서 72~120시간 동안 발효시키는 과정을 통해 항산화물질이 풍부하게 포함된 식물생리활성제가 제조된다. A plant bioactive agent rich in antioxidants is produced through the process of inoculating 1 to 10 parts by weight of salt-tolerant microorganisms into the medium and fermenting at 25 to 35°C for 72 to 120 hours.
식물생리활성제에 대해 항산화 평가 (DPPH assay)를 실시한 결과를 도 2에 보였다. The results of antioxidant evaluation (DPPH assay) on plant biological active agents are shown in Figure 2.
본 발명에 의해 제조된 식물생리활성제(EngreenSol™)을 농도 별로 처리하였을 때, 농도의존적으로 DPPH 항산화 활성을 보였으며, 500ppm의 농도에서 최대 80.98%의 항산화 활성을 보였다. 비교군으로 EGCG에 의한 DPPH 항산화 활성을 표시하였으며, 본 발명의 식물생리활성제 250ppm과 비슷한 DPPH 항산화 활성을 나타낸다. When the plant bioactive agent (EngreenSol™) prepared by the present invention was treated at different concentrations, it showed DPPH antioxidant activity in a concentration-dependent manner, and showed antioxidant activity of up to 80.98% at a concentration of 500 ppm. As a comparison group, the DPPH antioxidant activity by EGCG was shown, and it showed a DPPH antioxidant activity similar to 250ppm of the plant bioactive agent of the present invention.
도 3은 본 발명에 의해 제조된 식물생리활성제를 처리하여 재배한 시금치의 외관을 보여준다. 잎의 면적이 성인의 주먹 내지 손바닥 정도(가로 세로 7~10cm 정도)로 크게 성장한 것을 볼 수 있다.Figure 3 shows the appearance of spinach grown by treating the plant bioactive agent prepared according to the present invention. You can see that the leaf area has grown significantly to the size of an adult's fist or palm (about 7 to 10 cm in width and height).
도 4는 본 발명의 식물생리활성제를 투여하여 재배한 시금치, 당근, 깻잎의 외관과 베타카로틴 함량을 기재한 사진이다.Figure 4 is a photograph depicting the appearance and beta-carotene content of spinach, carrots, and perilla leaves grown by administering the plant bioactive agent of the present invention.
100g 당 베타카로틴의 함유량에서, 본 발명의 식물생리활성제를 사용하여 재배한 시금치는 222,946μg으로, 그렇지 않은 비교군의 시금치의 2,876μg에 비해 77 배 이상의 고함량을 보였다. 당근과 깻잎도 5516μg, 7040μg의 베타카로틴 함량을 보여, 본 발명의 식물생리활성제는 베타카로틴 함량을 크게 높이는 효과를 보였다. In terms of beta-carotene content per 100g, spinach grown using the plant bioactive agent of the present invention was 222,946 μg, which was 77 times higher than the 2,876 μg of spinach in the comparison group. Carrots and perilla leaves also showed beta-carotene contents of 5516 μg and 7040 μg, and the plant bioactive agent of the present invention showed the effect of significantly increasing the beta-carotene content.
도 5는 본 발명의 식물생리활성제를 투여하여 재배한 시금치의 영양성분 분석 표이다. 베타카로틴의 함량이 두드러지게 높은 것을 시험성적서를 통해 확인하였다. Figure 5 is a nutrient analysis table of spinach grown by administering the plant bioactive agent of the present invention. It was confirmed through the test report that the beta-carotene content was noticeably high.
도 6은 본 발명의 식물생리활성제를 투여하여 재배한 말린 고추의 외관과 비교군과 대비를 보여주는 표이다. Figure 6 is a table showing the appearance of dried peppers grown by administering the plant biological active agent of the present invention and the comparison with the comparison group.
본 발명의 식물생리활성제를 투여하여 재배한 고추의 수확량(4284kg/acre)은 그렇지 않은 경우(1836kg/acre)에 비해, 2.33배 정도 더 많았다. 그만큼 더 크고 품질이 우수한 개체로 생장하였다. The yield of peppers grown by administering the plant bioactive agent of the present invention (4284 kg/acre) was about 2.33 times higher than that of peppers grown without administration (1836 kg/acre). It grew into a larger and higher quality individual.
단위면적(1acre) 당 생산비는 본 발명의 식물생리활설제를 투여할 경우, 7131$로 그렇지 않은 경우(비교군: 본 발명의 식물생리활성제를 투입하지 않은 경우를 뜻함)의 5077$에 비해 140% 정도 증가한다. 그러나 소득은 118,238$로 비교군의 50674$에 비해 233% 증가한다. 이는 작물의 가격을 더 높게 책정할 수 있고, 단위 무게당 가격으로 판매할 경우, 무게가 더 나가기 때문에 더 높은 가격을 받을 수 있기 때문이다. 그에 따라 단위면적당 수익은 본 발명에 의한 것이 111,107$/acre로 비교군의 4,5597$/acre에 비해 244% 정도 더 증가한다.The production cost per unit area (1 acre) is $7,131 when the plant bioactivator of the present invention is administered, compared to $5,077 in the case where the plant bioactivator of the present invention is not administered (comparison group: meaning the case where the plant bioactivator of the present invention is not added). It increases by about %. However, income is $118,238, an increase of 233% compared to $50,674 in the comparison group. This is because the price of the crop can be set higher and, if sold at a price per unit weight, a higher price can be received because it weighs more. Accordingly, the profit per unit area according to the present invention is $111,107/acre, which is an increase of about 244% compared to $45,597/acre for the comparison group.
도 7은 본 발명의 식물생리활성제를 투여하여 재배한 마늘의 외관과 성분 분석 결과를 보여주는 도면이다.Figure 7 is a diagram showing the appearance and component analysis results of garlic grown by administering the plant biological active agent of the present invention.
본 발명의 식물생리활성제를 처리한 경우, 비교군 대비 단위면적당 수율은 20% 정도 증가하고, 알리신 함량은 150mg/100g으로 비교군의 33.5mg/100g에 비해 4.5배 정도 증가한다.When treated with the plant biological active agent of the present invention, the yield per unit area increases by about 20% compared to the comparison group, and the allicin content increases to 150 mg/100g, about 4.5 times compared to 33.5 mg/100g in the comparison group.
도 8은 본 발명의 식물생리활성제를 투여하여 재배한 상추의 외관과 분석 결과를 보여주는 사진이다.Figure 8 is a photograph showing the appearance and analysis results of lettuce grown by administering the plant biological active agent of the present invention.
외관 상, 본 발명의 식물생리활성제를 투여하여 재배한 상추의 생장이 더 우수한 것을 볼 수 있다. 성분 분석 결과, 본 발명의 것은 베타카로틴이 112,697μg/100g으로 비교군의 1040μg/100g에 비해 108배 정도 더 고함량을 보였다. In appearance, it can be seen that the growth of lettuce grown by administering the plant bioactive agent of the present invention is superior. As a result of component analysis, the beta-carotene of the present invention was 112,697μg/100g, which was 108 times higher than the 1040μg/100g of the comparison group.
도 9는 본 발명의 식물생리활성제를 투여하여 재배한 생고추의 외관과 분석 결과를 보여주는 사진이다.Figure 9 is a photograph showing the appearance and analysis results of raw peppers grown by administering the plant biological active agent of the present invention.
본 발명의 식물생리활성제를 투여하지 않은 경우, 고추 100g 당 베타카로틴의 함량은 456μg인데 반해, 본 발명의 것은 154,887μg, 135,743μg으로, 339배 더 증가한 함량을 보였다. When the plant bioactive agent of the present invention was not administered, the content of beta-carotene per 100 g of pepper was 456 μg, while that of the present invention was 154,887 μg and 135,743 μg, showing a 339-fold increase in content.
따라서 본 발명의 식물생리활성제는 작물의 생장을 촉진하여 작물의 크기를 크게하고, 항산화물질, 특히, 베타카로틴의 함량을 대략 100배 정도 증가시키는 효능을 보였다. 이는 염분이 제거된 해양심층수 또는 간수에 포함된 다량의 미네랄과 해조류 성분과 내염성 유산균에 의한 발효 작용이 만들어낸 예기치 못한 결과이다.Therefore, the plant biological active agent of the present invention promoted the growth of crops, increased the size of crops, and showed the effect of increasing the content of antioxidant substances, especially beta-carotene, by approximately 100 times. This is an unexpected result created by the fermentation action of a large amount of minerals and seaweed components and salt-tolerant lactic acid bacteria contained in deep sea water or bittern water from which salt has been removed.
상술된 사항에서 별도의 정의가 없는 경우, 본 명세서에서 사용된 모든 기술 및 과학적 용어는 본 발명이 속하는 기술분야에서 숙련된 전문가에 의해서 통상적으로 이해되는 것과 동일한 의미를 가진다. 또한, 일반적으로 사용되는 사전에 정의되어 있는 용어들은 명백하게 특별히 정의되어 있지 않은 한 이상적으로 또는 과도하게 해석되지 않는다. Unless otherwise defined in the above description, all technical and scientific terms used in this specification have the same meaning as commonly understood by an expert skilled in the technical field to which the present invention pertains. Additionally, terms defined in commonly used dictionaries are not to be interpreted ideally or excessively unless clearly specifically defined.
명세서 전체에서 어떤 부분이 어떤 구성요소를 "포함"한다 또는 "가지다"라고 할 때, 이는 특별히 반대되는 기재가 없는 한 다른 구성요소를 제외하는 것이 아니라 다른 구성요소를 더 포함할 수 있는 것을 의미한다. 또한, 단수형은 문맥에 의해 복수형을 포함할 수 있다.Throughout the specification, when a part is said to “include” or “have” a certain element, this means that it does not exclude other elements but may further include other elements, unless specifically stated to the contrary. . Additionally, the singular form may include the plural form depending on the context.
본 발명의 권리는 위에서 설명된 실시 예에 한정되지 않고 청구범위에 기재된 바에 의해 정의되며, 본 발명의 분야에서 통상의 지식을 가진 자가 청구범위에 기재된 권리범위 내에서 다양한 변형과 개작을 할 수 있다는 것은 자명하다.The rights of the present invention are not limited to the embodiments described above but are defined by the claims, and those skilled in the art can make various changes and modifications within the scope of the claims. This is self-evident.
도면 부호 없음.No drawing designation.
Claims (8)
염분을 제거하고,
내염성 유산균을 접종하여 발효하여,
식물의 베타카로틴 성분 함량을 증강시키는 식물생리활성제의 제조방법.Deep ocean water, deep ocean water, bittern (Bittern, Nigari) obtained from seawater desalination plants or thermal power plants is purified using a carbon filter,
remove salt,
Fermented by inoculating salt-tolerant lactic acid bacteria,
Method for producing a plant biological activator that enhances the beta-carotene content of plants.
The method of claim 7, wherein the treatment with the plant bioactive agent is performed on spinach, peppers, carrots or garlic to amplify the content of beta-carotene.
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