KR20230118417A - Treatment of viral causes, glomerulonephritis and nephritis - Google Patents

Abstract

In summary, SARS-CoV-2 activates the immune system to release a large number of cytokines, including IL-6, which can increase vascular permeability and cause migration of fluid and blood cells, resulting in respiratory distress and respiratory failure. cause negative symptoms. In addition, the common cold or flu itself replicates and is released from infected cells in the body. Excessive reactive oxygen species are generated by pathogen virus vaccine invasion, respiratory infection through viruses, radiation, chemicals, etc., which destroys cell membrane lipids of normal cells, destroys protein structures, and transforms nucleic acid bases. After being vaccinated against the COVID-19 virus, pathogenic microorganisms can lead to glomerulitis and vasculitis. Organs (Representative Figure 1) Circulates to organs through blood vessels such as large, medium, and small vessels, as well as arteries, capillaries, and veins, and causes multi-organ diseases. To solve this problem, it is possible to treat glomerulonephritis and vasculitis by recombining 8 kinds of natural herbal medicines (branch, hwangbaek, mothbaekpi, mold, anti-inflammation, goodwill, gumac, plantain taxa) and decocting for 24 hours.

Description

{Treatment of viral causes, glomerulonephritis and nephritis}

According to the present invention, humans who normally catch a humanized influenza A virus (human influenza virus type A) usually have symptoms that can be fatal, including fever, cough, sore throat, myalgia, conjunctivitis, and in severe cases respiratory problems and pneumonia. When the cytokine storm is secreted excessively and causes disease, the risk of various vascular-related diseases such as angina pectoris, arrhythmia, and myocardial infarction increases. The kidney, called the kidney of our body, plays an important filter role. When glomerulonephritis is induced, current hospital treatment methods can cause cytokine release syndrome and neurotoxicity as adverse drug reactions, but these complications are mostly controlled with appropriate treatment. possible. However, in order to produce CAR-T therapeutics, CAR-T production through appropriate peripheral blood T cell collection is required. In this process, some patients may fail to produce CAR-T, which may make administration impossible. In addition, it takes about 4 weeks to produce CAR-T. If the underlying disease progresses a lot during this period and the patient's general condition worsens, it may be difficult to administer the drug. Glomerulonephritis and nephritis can occur in a primary form of unknown cause, and can appear in a secondary form in relation to other related diseases. In particular, glomerulonephritis has the characteristics of an autoimmune disease caused by an abnormal immune response in normal cells or body tissues, and causes the formation of granulomas due to immune complexes, autoantibodies, and cellular immunity due to abnormal immune function in the body. It is known to act as In particular, it can be caused by viral respiratory infections such as H1N1 influenza, H5N1 influenza, SARS-CoV-1, and SARS-CoV-2, influenza B, parainfluenza virus, and Ebola. It is also known to be caused by bacteria, viruses, fungal infections, toxins, and tumors. Depending on the location and extent of the affected blood vessels, various symptoms appear. Due to the nature of autoimmune disease, it is known that it cannot be prevented in advance. In order to solve this problem, 8 kinds of traditional herbal medicines of natural substances were recombined (Zhibuja, Hwangbaek, Mortice bark, Hyeonggae, Anti-inflammation, Good will, Gumaek, Plantain taxidermy), and the herbal medicines were refined and processed to remove toxicity, and 2ml of water and 30mg of each of 8 medicines were all It can be put in an electric medicine decoction machine and decocted for 24 hours to make and administer medicine to treat acute and chronic glomerulonephritis and nephritis diseases.

In particular, it can be caused by viral respiratory infections such as H1N1 influenza, H5N1 influenza, SARS-CoV-1, and SARS-CoV-2, influenza B, parainfluenza virus, and Ebola. It is also known to be caused by bacteria, viruses, fungal infections, toxins, and tumors. Serious organ problems can occur when the glomeruli are unable to remove body fluids and waste products. The disease can be caused by decreased kidney function, for example due to damage to the glomerulus. Anticancer drugs have low recovery rate, low selectivity of cancer cells, toxic side effects and drug resistance. Therefore, research is being conducted to develop new potent anticancer drugs. For example

♣ Disease: Dead glomeruli caused by major diseases and viral vaccines that can cause glomerulonephritis

Description: In fibrillary glomerulonephritis and nephritis diseases, abnormal proteins build up around the walls of blood vessels. Abnormal proteins can also cause nephrotic syndrome.

Prognosis: The prognosis is poor. Severe loss of kidney function (end-stage renal failure) in about half of the cases within 4 years

Current treatment: (corticosteroids and immunosuppressants) It is not clear whether this treatment is helpful.

[Prior art literature]

♣Disease: Rapidly progressive primary glomerulonephritis

Description: This group of disorders causes microscopic damage to the glomerulus and progresses rapidly. Sometimes an infection or other treatable condition causes this disorder. A kidney biopsy is required for diagnosis.

Prognosis: The prognosis is poor. End-stage renal failure develops within 6 months in at least 80% of untreated people. The prognosis is poorer in people younger than 60 and if the underlying condition causing glomerulonephritis responds to treatment. With current treatments, 43% of people develop end-stage renal disease within 12 months.

♣ Disease: Immunoglobulin A (IgA) nephropathy

Description: The most common form of glomerulonephritis in the world is caused by immune complexes (combinations of antigens and antibodies) that build up in the kidneys (currently in vasculitis and viral cases).

Prognosis: The disease usually progresses slowly. After 10 years, about 25% develop end-stage renal failure. The disorder progresses more slowly in children to end-stage renal failure after about 30 years.

♣ Disease: Membrane proliferative glomerulonephritis:

Description: This rare type of glomerulonephritis usually appears between the ages of 8 and 30 years. The cause of this disorder is immune complexes (combination of antigens and antibodies) attaching to the kidneys, but it is not known why the complexes sometimes attach to the kidneys. (for vascular cancer and viral glomerular loss)

Prognosis: In people whose cause is unknown, the prognosis is not very good. About half of people progress to end-stage renal failure within 10 years and 90% within 20 years.

Current Treatment Methods: It is not clear whether corticosteroids and immunosuppressants are helpful in treatment.

It is known that glomerulonephritis and nephritis cannot be prevented in advance due to the nature of the disease. In order to solve this problem, 8 kinds of natural substances were recombined (branch, hwangbaek, moth, hyeonggae, lobules, goodwill, gumac, plantain taeksa) to refine and process herbal medicines to remove toxicity, and 2ml of water and 30mg of each of 8 medicines were all in an electric medicine decoction machine. It can be put in and decocted for 24 hours to make and administer medicine to treat acute and chronic glomerulonephritis and nephritis diseases.

[Patent Literature]

Application number/date 1020140135836 (2014.10.08.)

Application number/date (international) 1020177037355 (2016.06.17)

[Non-Patent Literature]

Year of implementation: 2016 Name of department: Ministry of Education

Project name: Cultivation of academic successors Date of issue: 2017-06-26

Project title: Proof of efficacy and mechanism of natural products inhibiting glomerulosclerosis using herbal medicine prescription Host research institute: Wonkwang University

Total research period: 2014-06-01 ~ 2017-05-31

[Contents of the invention]

In particular, it can be caused by viral respiratory infections such as H1N1 influenza, H5N1 influenza, SARS-CoV-1, and SARS-CoV-2, influenza B, parainfluenza virus, and Ebola. It is also known to be caused by bacteria, viruses, fungal infections, toxins, and tumors. Depending on the location and extent of the affected blood vessels, various symptoms appear. There are various diseases depending on glomerulitis and vasculitis, but it is known that it cannot be prevented in advance due to the nature of autoimmune disease. In order to solve this problem, 8 kinds of natural natural substances and traditional herbal medicines are recombined (Zhibuja, Yellowbaek, Mortice bark, Hyeonggae, Anti-inflammation, Goodwill, Gumaek, and Plantain), trimmed and processed to remove toxicity, pour 2ml of water in an electric decoction, and put all 8 herbal medicines. It is possible to easily treat acute and chronic glomerulonephritis and nephritis disease by decocting it for about 24 hours and making or administering herbal medicine.

[Problem to be solved]

The present invention is a chronic systemic inflammation (SI), diabetes, stress response, Cushing's syndrome ( hypersensitivity), immune-mediated diseases, and granulomatous diseases. Atherosclerosis, necrosis, erythrocytic repopulating red blood cells (RBC), sarcoidosis, chronic myeloid cell leukemia (CMML), high numbers of monocytes are severe infections, sepsis, high numbers of monocytes atherosclerosis in the areas of cardiovascular in risk groups It can be caused by a viral respiratory infection. It is also known to be caused by bacteria, viruses, fungal infections, toxins, and tumors. In the present invention, glomerulonephritis and pyelonephritis are recombined with 8 kinds of natural substances (branch, yellow white, moth bark, mold, anti-inflammation, goodwill, gumac, plantain taxa), and herbal medicines are trimmed and processed to remove toxicity, and 2 ml of water and 8 drugs Put all 30mg of each in an electric medicine decoction machine and decoct it for 24 hours to make medicine and administer it, and treat acute and chronic glomerulonephritis and nephritis disease.

What is known as a "cytokine storm" triggers a massive inflammatory response that can lead to severe pathological consequences. When inflammation is created in blood vessels, the risk of various vascular-related diseases such as angina pectoris, arrhythmia, and myocardial infarction increases. In addition, the blood vessels going to the brain are blocked by inflammation, and when the blood vessels are damaged, various symptoms such as cerebral infarction, spinal cord damage, paresthesia, and numbness in the hands and feet may appear. In addition, various symptoms such as chronic shortness of breath, pulsating headaches such as throbbing blood vessels in the head like a pulse, chronic and acute glomerulonephritis, inflammatory sinusitis, and visual disturbances are also associated with diseases that may appear when symptoms of vasculitis persist. It becomes applicable. The main symptoms of glomerulonephritis and nephritis appear in various ways depending on the onset blood vessels, and cannot be prevented in advance due to the nature of autoimmune diseases. In order to solve this problem, 8 kinds of natural natural substances traditional herbal medicines (branch, hwangbaek, mothbaekpi, hyeonggae, anti-inflammation, good will, gumac, plantain taxa) were trimmed, processed, and recombined, and 2ml of water and 8 kinds of herbal medicines were all 30mg each in an electric medicine brewer. It can be easily treated with acute and chronic glomerulonephritis and nephritis when it is put in and decocted for 24 hours to make a medicine and administer it.

When blood vessels, which play an essential role in maintaining life, have problems, various related diseases such as various coronary artery diseases, arteriosclerotic diseases, and peripheral vascular diseases occur. It is one of the diseases caused by problems with blood vessels caused by virus vaccines. It is a disease in which inflammation of the blood vessel walls causes ischemia and damage to tissues that have been supplied with nutrients. Depending on the nature and location of the inflammation, symptoms differ from each other. There are about 1 million glomeruli per kidney in our body. Blood vessels, which are divided into arteries, capillaries, and veins, play an important role in circulating oxygenated blood between the heart and each organ and tissue in the body. , Recombinant 8 kinds of natural natural substances herbal medicines (branch, hwangbaek, moth bark, hyeonggae, lobules, goodwill, gumac, plantain taeksa) to trim and process drugs to remove toxicity, 2ml of water and 30mg each of 8 herbal medicines in an electric medicine brewer Put all of them in and decoct them for 24 hours to make a medicine and when administered, you can easily treat acute and chronic glomerulonephritis and nephritis diseases.

The drawings are divided by drug.
Figure 1 shows the structure of a large artery.
Figure 2 shows eight kinds of herbal medicines (A ~ H)
Figure 3 shows a more specific artery
In the present invention, blood vessels divided into capillaries and veins, including arteries, play an important role in circulating oxygen-containing blood between the heart and each organ and tissue in the body, such as common cold, influenza, viruses, and respiratory diseases through infection. I'm doing If there is a problem with the blood vessels, which are essential for maintaining life, various related diseases such as various coronary artery diseases, arteriosclerotic diseases, and peripheral vascular diseases will occur. In addition, some strains not only proliferate in the body by avoiding the immune system attack, but also induce an inflammatory response in blood vessels, which can cause glomerulonephritis. One of the diseases caused by problems with these blood vessels when glomerulitis occurs due to viral vaccines is inflammation of the blood vessel walls, which causes ischemia and damage to tissues that have been supplied with nutrients. Depending on the nature and location of the inflammation, symptoms differ from each other. It can induce a massive inflammatory response and lead to serious pathological consequences.

In this experiment, each drug was divided into 8 types (examples), and the drug name, drug discrimination, drug analysis through content test method, and drug results were recorded. Glomerulonephritis refers to a disease in which the glomeruli, which play an important filter role in the kidneys, called the kidneys of our body, are inflamed. Glomerulonephritis symptoms can be confirmed through daily urine, so it is important to pay attention to your body.

The glomerulus is a tissue made up of a mass of capillaries from the renal artery. There are 2 million of them, 1 million per kidney. The glomerulus functions as a filter that filters out excess fluid, electrolytes, and urinary toxins in the body. Problems with the glomeruli may prevent them from removing excess fluid or waste products from the body. At this time, glomerulonephritis may appear acutely suddenly or slowly and chronically. Its types include IgA nephritis, idiopathic crescentic glomerulonephritis, and C3 glomerulonephritis. Secondary glomerulonephritis is a disease caused by an infection of some disease, and there are glomerulonephritis caused by viral infections such as strep infection, endocarditis, HIV, hepatitis B, and C. In the case of glomerulonephritis, there is no specific prophylaxis. That's why it's important to know the symptoms of glomerulonephritis and pay attention to it in advance and manage it. In the present invention, the treatment method for glomerulonephritis is to recombinant 8 kinds of natural natural drug herbal medicines (branch, yellow white, moth bark, mold, anti-inflammation, goodwill, gumac, plantain taxa) to trim and process the herbal medicine to remove toxicity, 2 ml of water and drugs Figure 2A It is a method of treating with a recombinant drug by putting it in ~H and decocting it for 24 hours in an electric medicine decoction machine.

[Example]

Branches, Kochiae Fructus

Note: It refers to preparations produced according to the prescribed prescription, production technology, and quality using herbal ingredients as raw materials under the theory of traditional Chinese medicine. Note: The 2010 edition of “Chinese Pharmacopoeia contains 1,063 neutral medicines. In 2009, 102 types of neutral medicines were included in the 「National Inventory」, accounting for 33% of the total types of medicines. Chinese neutral medicines have some classic prescriptions, but most are new prescriptions based on classic prescriptions.

Botanical Name: Tapsari Application Number: 10-2000-0037815

Scientific name: Kochia scoparia Schrader

Latin name: Kochiae Fructus

Scientific name: Kochia scoparia (L.) Schrad. var. scoparia; Bassia scoparia (L.) A.J. Scott; Atriplex scoparia (L.) Crantz; Bassia sicorica (O. Bol□s & Masclans) Greuter &Burdet; Bassia sieversiana (Pall.) W.A.Weber; Bushiola scoparia (L.) Nieuwl.; Chenopodium scoparia L.; Kochia densiflora Turcz. ex Aellen; Kochia parodii Aellen; Kochia scoparia (L.) Schrad.; Kochia sicorica O. Bol□s &Masclans; Kochia sieversiana (Pall.) C.A.Mey.; Kochia trichophylla Hort.; Salsola scoparia (L.) M. Bieb.; Suaeda sieversiana Pall.; Bassia scoparia var. culta Voss; Bassia scoparia subsp. culta (Voss) Nebot &al.; Bassia scoparia subsp. densiflora (Turcz. ex Aellen) Cirujano &Velayos; Bassia scoparia var. subvillosa (Moq.) Lambinon; Bassia scoparia var. trichophylla (Voss) S.L. Welsh; Kochia parodii var. contracta Aellen; Kochia parodii var. densa Aellen; Kochia parodii var. elongata Aellen; Kochia parodii var. glabrescen Aellen; Kochia prostrata var. densiflora Moq.; Kochia scoparia var. alata Blom; Kochia scoparia subsp. densiflora (Turcz. ex Aellen) Aellen; Kochia scoparia f. densiflora Moq.; Kochia scoparia var. pubescens Fenzl; Kochia scoparia f. remotiflora Beck; Kochia scoparia var. sieversiana (Pall.) Ulbr. ex Asch. & Graebn.

Synonym of drug: Leymaekjimaek, Anonymous 益明, Let's kill , Cheonduja, Jikyujiji, Nakchuja

Medicinal part: fruit

Efficacy class: Isu ginseng medicine for low back pain ( )

Classification: Goosefoot and Chenopodiaceae

Origin plant form: It is an annual herb and grows upright to 0.5~1.5m in height. Stem branches diverge a lot, and there are hairs on the upper part. Leaves alternate, lanceolate or narrow lanceolate, without petioles. Both ends are narrow and the edges are flat, with 3 veins, 2-5 cm long and 2-8 mm wide, with some long hairs. Flowers are small, yellowish green, forming spikes, and several of them hang together on the leaf axils in July-August.

Distribution: Widely distributed throughout the world including Korea

Identification: The surface is grayish green or light brown. It is flat ball-shaped or five-pointed star-shaped, 1-3 mm in diameter and about 1 mm thick. There are 5 membranous small wings on the edge. In the center of the ventral side, there is a slightly protruding dot-shaped trace of the fruit sack and there are radial veins.

Main ingredients: Momordin Ic, Oleanolic acid, 20-Hydroxyecdysone

Preclinical: anti cancer, dermatitis in vivo, antiinflammatory

Harvesting and Timing: Harvested in the fall when the fruits are ripe

Basic processing: Dry in the sun and shake off to collect only fruits (pods).

Method of making cloth: Remove impurities and use raw or infuse.

Yellow and white, (黃柏), Phellodendri Cortex

Botanical name: Hwangbyeok tree Application number: 10-2013-0071543 Application number: 10-2006-0018305

Scientific name: Phellodendron amurense Ruprecht

Latin name: Phellodendri Cortex

Identification of Yellow White Tree: It is a deciduous broad-leaved tree that grows well in deep, fertile places rather than in dry areas.

Form of Origin: It is a deciduous tree, about 10-25m high. There is a thick and soft cork layer split horizontally and vertically on the stem bark. The inner surface of the harvested stem bark is bright yellow. Leaves opposite, odd pinnate leaves, 5-13 leaflets. Drupes resembling fruit berries are round and become purplish black when ripe.

Distribution : Korea, China (Inner Mongolia, Liaoning, Shandong, Shanxi, Anhui, Jilin, Henan, Hebei, Heilongjiang), Japan, Taiwan, Russia (Far East)

Identification: Plate-shaped to semi-tubular pieces with irregular length and width. The outer surface is yellowish green or brown, and traces of irregular vertical wrinkles and small pores are occasionally seen. The inside is yellow or tan, and thin vertical lines can be seen and it is smooth. After peeling, the outer surface is bright yellow with clear traces of pores. The inner surface is bright yellow, and thin vertical lines can be seen. It is a voluminous section. The cross-sectional structure is denser than that of Cheonhwangbaek. It is better to have a uniformly thick peel, a small cork layer, and a dark and clear yellow color on the cross section.

Botanical name: (Hwangpisu)

Scientific name: Phellodendron chinense Schneider

Latin name: Phellodendri Cortex

Identification of yellow bark: As a tree, it grows in the mixed forest of broad-leaved trees over 900 meters.

Form of Origin: It is a deciduous tree, about 10-12m high. The stem bark is dark brown, very thin and has no elasticity. There is no thick cork layer and the inner surface is yellow. Both the inner surface and the body of the harvested stem bark are bright yellow. Leaves face each other, odd pinnate leaves. The number of leaflets is 7-15. The back side is white and the leaf veins are distinct. A long nanny is seen throughout. It is a flower panicle. Drupes resembling fruit berries are densely ball-shaped, ripe, purple-black.

DISTRIBUTION: China (Gansu, Guangdong, Guangxi, Guizhou, Shaanxi, Sichuan, Anhui, Yunnan, Jiangsu, Zhejiang, Fujian, Henan, Hunan, Hubei)

Drug synonyms: Cheonbaek (川檗), 黄蘗, Hwangbaek (黃檗), Baekpi (檗皮), Cheonhwangbaek (川黃柏), Hwangbaekpi (黃檗皮), Hwangbaek (黃栢), Bekmok (檗木)

scientific name; Phellodendron chinense C.K. Schneid. ; Phellodendron fargesii Dode; Phellodendron macrophyllum Dode

Medicinal parts: Stem bark (bark)

Identification: Slightly curved plate-shaped or cloth-shaped (淺槽狀), length and width are not regular. Externally, it is similar to the Guan Huang Baek, but it is yellowish brown and darker than the Guan Huang Baek. It is similar to the Guan Hwang bag on the inside, but it is dark yellow compared to the Guan Hwang bag. It is a voluminous section. The cross-section structure is layered and less dense than that of the Guan Huang bag.

Collection and timing: Collected in March-June from trees over 10 years old

Basic processing: Peel off the cork layer before it dries, cut it into appropriate sizes, and dry it in the sun.

Fabrication method: Hwangbaek (黃栢): Remove miscellaneous substances from the raw material, scrape off the cork layer, distinguish according to thickness, and wash off soil and dust with water. After sealing it for 8 to 12 hours and dampening it, when the internal and external humidity is the same, it is cut into about 3mm thick (width) (fisa) and dried in the sun or over a fire.

Orange (酒黃柏): Sprinkle a certain amount of yellow wine (黃酒) evenly on clean yellow-white filament (皮絲), seal it for about 1-2 hours, moisten it, put it in a container for cooking (炒製) and use it as a culture (literature fire). Vinegar to make the surface dark yellow, take it out when it is slightly moist, and let it cool in the shade (10kg of yellow wine per 100kg of yellow and white skin).

Yeomhwangbaek (鹽黃柏): Sprinkle a certain amount of saline solution evenly on clean yellowbaek cloth (黃柏皮絲), moisten it and soak it in a little, put it in a container for herbicides, and the surface is brownish yellowish with culture (文fire). Take it out when it is slightly moist by touching it with your hands while vinegaring it so that it turns red and yellow, and let it cool in a cool shade (2.5kg of table salt per 100kg of hwangbaekpi).

Hwangbaekpi (黃柏炭): Put clean yellowbaek pisa (黃柏皮絲) in a container for herbicides, and burn it with a fire to the extent that the surface is grayish black and the inside is brown, and the medicinal properties remain. After boiling, sprinkle some clear water to extinguish the flame, then take it out and leave it to cool in a cool shade.

Poje Purpose: Sexuality (性寒) Miho (味苦) Upsin bladder (入腎膀胱經). Clear heat control dampness(淸熱燥濕) detoxification of dead fire(瀉fire解毒)→between damp heat(濕熱瀉痢), jaundice(黄疸), clams(帶下), heat( ), beriberi, osteoporosis, fever, apoplexy, oil well, sore throat, eczema itching, etc.

Raw product: It has excellent advantages in acupuncture, clearing heat, regulating humidity, and detoxifying hot and cold symptoms. ), eczema, dysentery, and jaundice

Orange Baek (酒黃柏): Coldness (寒性) is relieved, moist heat (濕熱) cheong (淸) and joint (關節) tongri (通利) action is enhanced. In addition, thanks to the ascending effect of alcohol, it is possible to clear heat (淸熱) by leading medicine and ascending (fever fever), so it is possible to clear the fever due to fever (熱壅上焦).症) and foot condition ( ) used, etc.

Yeomje (鹽製): It alleviates the weakness of high tide, does not damage the stomach, and has the advantage of strengthening consonants, so it is good for Shinheohwahwang (腎虛fire旺), gastric emptying ( ), colic (帶下), back pain between the bones, etc. Also, by making the medicine go down, the action of hydration (瀉相火) ) strengthens the action of clearing the column of heat (濕熱).

Tanje (炭製): Because the effect of yanghyeol hemostasis (凉血止血) is enhanced, it is mainly used for changing blood, urinary bleeding, and rupture.

Experiment contents

Hwang Baek (Gi-dong) Lee Lee, Go Cheoncheonju 柱, Yeom 廉yeon .) Changes in chemical composition before and after wrapping. )

Note: It refers to preparations produced according to the prescribed prescription, production technology, and quality using herbal ingredients as raw materials under the theory of traditional Chinese medicine. The 2010 edition of “Chinese Pharmacopoeia contains 1,063 neutral medicines. In 2009, 102 types of neutral medicines were included in the 「National Inventory」, accounting for 33% of the total types of medicines. Chinese neutral medicines have some classic prescriptions, but most are new prescriptions based on classic prescriptions.

A. Content measurement:

Experiment target : Palmate chloride

Pore recipe: 140 degrees

Pollination method: Hongje for 40 minutes

Solvent: Methanol

Analytical instrument: HPLC

Analysis conditions: Y= 10527 X-88424, r =0.9996, 0.00956~0.1912 μg

Result: Criterion

Value :0.00%→0.1

B. Content measurement:

Experiment target : Palmate chloride

Pore recipe: 170 degrees

Packaging method: 40 minutes of heat

Solvent: Methanol

Analytical instrument: HPLC

Analysis conditions: Y= 10527 X-88424, r =0.9996, 0.00956~0.1912 μg

Result: 5% increase

Value: 0.00%→1.2

C. Content measurement:

Test target: Palmatine chloride

Formation method: 180 degrees

Fabrication method: temperature 200 degrees, heating time 2 minutes

Solvent: Ethanol

Analytical instrument: HPLC

Analysis conditions: Y=4131597.0305X+9112.4944, r=0.9991, 0.006~0.09μg

Result: 2% increase

Value: 0.00%→1.6

D. Content measurement:

Experiment target : Palmate chloride

Pore recipe: 10 minutes

Fabric manufacturing method: Processing at 180 degrees

Solvent: Ethanol

Analytical instrument: HPLC

Analysis conditions: Y=4131597.0305X+9112.4944, r=0.9991, 0.006~0.09μg

Result: 4% increase

Value: 0.00%→0.2

D. Content measurement:

Experiment target: Palmate chloride

Pore recipe: 60 minutes

Fabric manufacturing method: Processing at 180 degrees

Solvent: Ethanol

Analytical instrument: HPLC

Analysis conditions: Y=4131597.0305X+9112.4944, r=0.9991, 0.006~0.09μg

Result: 6% increase

Value: 0.00%→1.8

E. Content measurement:

Experiment target: Palmate chloride

Pore recipe: 120 minutes

Fabric manufacturing method: Processing at 180 degrees

Solvent: Ethanol

Analytical instrument: HPLC

Analysis conditions: Y=4131597.0305X+9112.4944, r=0.9991, 0.006~0.09μg

Result: 8% increase

Value: 0.00%→1.5

1. Content measurement:

Experiment target : Palmate chloride

Four Recipe:

Fabrication method: temperature 200 degrees, heating time 2 minutes

Solvent: 60% ethanol

Analytical instrument: HPLC

Analysis conditions: Y=4131597.0305X+9112.4944, r=0.9991, 0.006~0.09μg

No result

Figure: 2.21

2.1. Content measurement:

Experiment target : Palmate chloride

Four Recipe:

Fabrication method: temperature 240 degrees, heating time 4 minutes

Solvent: 60% ethanol

Analytical instrument: HPLC

Analysis conditions: Y=4131597.0305X+9112.4944, r=0.9991, 0.006~0.09μg

No result

Figure: 0.4689

3.1. Content measurement:

Experiment target : Palmate chloride

Four Recipe:

Fabrication method: temperature 280 degrees, heating time 2 minutes

Solvent: 60% ethanol

Analytical instrument: HPLC

Analysis conditions: Y=4131597.0305X+9112.4944, r=0.9991, 0.006~0.09μg

No result

Figure: 1.117

4.1. Content measurement:

Experiment target : Palmate chloride

Four Recipe:

Fabrication method: temperature 280 degrees, heating time 6 minutes

Solvent: 60% ethanol

Analytical instrument: HPLC

Analysis conditions: Y=4131597.0305X+9112.4944, r=0.9991, 0.006~0.09μg

No result

Numerical value: 0.01266

5.1. Content measurement:

Experiment target : Palmate chloride

Four Recipe:

Fabrication method: temperature 240 degrees, heating time 2 minutes

Solvent: 60% ethanol

Analytical instrument: HPLC

Analysis conditions: Y=4062106.7708X-12224.2018, r=0.9993, 0.00526~0.1578μg

No result

Number: 2.493

6.1. Content measurement:

Experiment target : Palmate chloride

Four Recipe:

Fabrication method: temperature 240 degrees, heating time 6 minutes

Solvent: 60% ethanol

Analytical instrument: HPLC

Analysis conditions: Y=4062106.7708X-12224.2018, r=0.9993, 0.00526~0.1578μg

No result

Figure: 0.443

7.1. Content measurement:

Experiment target : Palmate chloride

Four Recipe:

Fabrication method: temperature 280 degrees, heating time 2 minutes

Solvent: 60% ethanol

Analytical instrument: HPLC

Analysis conditions: Y=4062106.7708X-12224.2018, r=0.9993, 0.00526~0.1578μg

No result

Figure: 1.844

7.1. Content measurement:

Experiment target : Palmate chloride

Four Recipe:

Fabrication method: temperature 280 degrees, heating time 6 minutes

Solvent: 60% ethanol

Analytical instrument: HPLC

Analysis conditions: Y=4062106.7708X-12224.2018, r=0.9993, 0.00526~0.1578μg

No result

Numerical value: 0.02257

Mortice, (桑白皮), Mori Radicis Cortex

Botanical name: Mulberry Application number: 10-1992-0007991 Application number: 10-2011-0027789

Scientific name: Morus alba Linn

Team name: Mori Radicis Cortex

Drug synonyms: epithelium, upper root skin, adverb, upper root skin, white epithelium, soft tongue, food sleep ( ), upper root white skin

Scientific name: Morus alba L.; Morus alba L.; Morus acidosa Griff.; Morus atropurpurea Roxb.; Morus australis Poir.; Morus bombycis Koidz.; Morus cavaleriei H.L□v.; Morus chinensis Lodd. ex Loudon; Morus formosensis Hotta; Morus hastifolia F.T.Wang & T.Tang ex Z.Y.Cao; Morus intermedia Perr.; Morus inusitata H.L□v.; Morus latifolia Poir.; Morus longistylus Diels; Morus multicaulis Perr.; Morus nigriformis (Bureau) Koidz.; Morus tatarica L.; Morus alba var. atropurpurea (Roxb.) Bureau; Morus alba var. bungeana bureau; Morus alba var. indica Bureau; Morus alba var. latifolia (Poir.) Bureau; Morus alba var. multicaulis (Perr.) Loudon; Morus alba var. nigriformis bureau; Morus alba var. stylosa Bureau; Morus alba var. tatarica (L.) Ser.; Morus australis var. hastifolia (F.T.Wang & T.Tang ex Z.Y.Cao) Z.Y.Cao; Morus australis var. incisa C.Y. Wu; Morus australis var. inusitata (H. L v.) C. Y. Wu; Morus australis var. linearipartita Z.Y. Cao; Morus australis var. oblongifolia Z.Y. Cao; Morus bombycis var. angustifolia Koidz.; Morus bombycis var. bifida Koidz.; Morus bombycis var. longistyla Koidz.; Morus bombycis var. tiliifolia Koidz.; Morus stylosa var. ovalifolia Ser.

Medicinal part: root bark (root skin, root skin)

Classification: Moraceae Moraceae

Ecology: As a deciduous tree or shrub, it is planted near villages.

Plant Origin: Deciduous tree or shrub. Stem bark is grayish white, and small branches are grayish brown or grayish white. Branches have a hole in the center and are filled with white water. Leaves alternate with single leaves, ovate circular or long elliptical ovate, split into 3 to 5, with dull sawtooth on the edge. It blooms in June as a dioecious flower, and the male inflorescence hangs on the caudal inflorescence that droops downward from the leaf axil at the lower part of the branch. The fruit is 1~2.5cm long and ripens black in June.

Distribution : Korea, China, Japan, other Asia, Europe

Identification: Slightly curved root bark, tubular, semi-tubular or belt-shaped, 1-6 mm thick and irregular in length. The surface is white to light yellowish brown, and there are also yellowish brown or yellow scale-like bark. It is easy to fall off, has many fine vertical wrinkles, and has many reddish-brown skins. The inner surface is yellowish white or grayish yellow with thin vertical wrinkles.

Anti-inflammatory effect: inhibition of nitric oxide production

Nephropathy: Prevention of renal damage

Hepatitis: inhibition of hepatitis B virus (HBV) DNA replication

Stroke: inhibition of collagen- and arachidonic acid-induced platelet aggregation and TXB(2) formation in platelet aggregation culture, inhibition of thrombus formation for collagen-induced TXB(2) formation,

plague or parasitic disease

Antibacterial effect: No toxicity of moth extract Anti-microbial activity Moderately active.

: Inhibit the growth of dental caries bacteria such as Streptococcus sobrinus, Streptococcus sanguis, Porpyromonas gingivalis periodontitis,

Antiviral effect: (herpes simplex type 1 virus) HSV-1 vero cells antiviral effect

Anti-cancer effect: tumor

1. THP-1 Human Leukemia Cells?

2. Decreases the viability of SW480 cells in a concentration-dependent manner and induces apoptosis

3. Survival of prostate cancer cells

4. Reduction of nuclear accumulation, DNA binding and STAT3 activity by inhibiting phosphorylation

5. · Prostate pressure cell apoptosis induction.

6. HL-60, Hela, HepG-2, A-549 and AGS cell selective cytotoxic effect

7. Cytotoxic activity of K-562, B380 human leukemia cells, B16 mouse melanoma cells, and apoptosis effect of cells exposed to Mortalis extract (DNA fragmentation, PARP cleavage, and nuclear condensation)

8. human colorectal cancer cells

9. Inhibition of cell growth

10. Inhibition of apoptosis

Diseases of the blood and blood-producing organs, related to the immune system

11. Skinning effect: Enhances the proliferation of splenic lymphocytes in the presence of mitogens, suppresses mainly B cell IgM antibody production,

12. Leukemia: induces apoptosis of human leukemia (HL60)

13. Pulmonary inflammation: Suppression of IL-6 production by lung epithelial cells (A549) and NO production (MH-S) by lung macrophages.

14. Antithrombotic effect: Concentration-dependent inhibition of collagen-induced platelet aggregation

anti-asthmatic effect

1. The mouse asthma model. Increased production of Foxp3+ Tregs and enhanced Foxp3+ mRNA expression

2. AHR response, eosinophil infiltration, serum histamine, reduction of OVA-specific IgE production, suppression of Th2 cytokines

3. Decreased levels of bronchoalveolar lavage fluids, suppressed influx of inflammatory cells, thickened epithelium of bronchioles, mucus, and collagen accumulation

4, ovalbumin (OVA) induced allergic asthma mice model

Reduction of OVA-specific IgE and IL-4, IL-5, and IL-13 in serum

Harvesting and Timing: Harvest the roots from when the leaves fall in late autumn until they sprout in the spring of the following year.

Basic processing: Remove the yellowish brown cork, split it lengthwise, peel off the root bark, and dry it in the sun.

Fabric: Mothbaekpi (桑白皮): After picking out impurities from the raw material, wash it thoroughly in fresh water, seal it for about 8-12 hours, and moisten it. dried in the sun

: Wheatgrass Baekpi (蜜桑白皮): Pour a certain amount of soft wheat (練蜜) with an appropriate amount of boiled water and dilute it, sprinkle it evenly on clean skin (皮絲), mix it, seal it for about 2-4 hours, moisten it Put it in a container and set it on fire. When the surface turns golden yellow and becomes soft and does not stick to your hand, take it out and let it cool in the shade. (桑白皮絲) per 100kg soft-ripened wheat (鍊熟蜂蜜) 25kg).

Characteristics of cloth: Sangbaekpi (桑白皮): It is a curved sajojang (絲條狀), about 6-25mm long and about 3mm wide.

The surface is light yellowish white or light yellow, and the surface is soft and light. It has a slight fragrance and the taste is sweet.

Poje Purpose: Sexual Cold (性寒), Maegam (味甘), Ippaegyeong (入肺經). Applied to dead lungs and pyeongcheon (瀉肺平喘, anti-hydrogen species) → waste heat (肺熱咳喘, sujongjangmanyo (水腫脹滿尿少), cotton-gap couple (面目肌膚浮腫), etc.

Raw materials: Raw products are cold in nature and have a strong ability to resist death, so they can be used in water species, urea, and cotton. (面目肌膚浮腫) is often used.

Milja (蜜炙): The nature of the cold (寒瀉) is alleviated, and the action of yunpyejihae (潤肺止咳) is strengthened, so it is widely applied in the case of ruined haemaecheon (肺虛咳喘)

Experiment details

It is to provide a pharmaceutical composition for the prevention and treatment of allergic diseases containing an extract of moth bark.

[Composition] Extract the moth bark powder with methanol at 70 degrees Celsius while stirring, combine the methanol extracts, remove methanol with a rotary vacuum concentrator, saturate the resulting precipitate with water, and filter to remove the precipitate. To the precipitate obtained by heating and concentrating this aqueous solution, the same amount of water and a mixed solvent of ethyl acetate were added, extracted twice, the extracts were mixed, the ethyl acetate layer fraction was removed using a separating funnel, only the aqueous layer fraction was filtered, and the water was concentrated by evaporation. A solid extract is obtained. Prevent allergic disease by administering this Mortice bark extract to allergic hypersensitivity patients

The present invention relates to an advanced glycation end product production inhibitor or health functional food capable of inhibiting the induction of diabetic complications containing a compound isolated from moth bark as an active ingredient, and specifically, the present invention relates to mulberofuran G ( mulberrofuran G), mulberrofuran K, kuwanon G, kuwanon Z, oxyresveratrol, 2', 4', 5, 7-tetrahydroxyflavanone (2' 4' , 5, 7- tetrahydroxyflavanone), morusignin L and dihydromorin

1. Content measurement:

Experiment target: total flavone

Production method: fresh

Fabrication method: dry grinding

Solvent: Ethanol

Analytical instrument: HPLC

Analysis condition: 200~800nm 324nm A=0.02143C-0.00458, r=0.9999 0~48.672g

Result: Criterion

Number: 3.284%

2. Content measurement:

Experiment target: total flavone

Production method: traditional secret method ( )

Packing method: Marinate 20g of mustard bark for 1 hour and then heat it in culture to turn it into a dark yellow color. If it does not stick to your hands, take it out and cool it.

Solvent: Ethanol

Analytical instrument: HPLC

Analysis condition: 200~800nm 324nm A=0.02143C-0.00458, r=0.9999 0~48.672g

Result: decrease

Number: 2.887%

3. Content measurement:

Experiment target: total flavone

Production method: Honghangang product

Fabrication method: Heat in a drying box at 150℃ for 20 minutes and then cool.

Solvent: Ethanol

Analytical instrument: HPLC

Analysis condition: 200~800nm 324nm A=0.02143C-0.00458, r=0.9999 0~48.672g

Result: decrease

Number: 2.890%

4. Content measurement:

Experiment target: total flavone

Pore manufacturing method: Mipi light wave

Wrapping method: Heat in a microwave oven for 7 minutes and then cool.

Solvent: Ethanol

Analytical instrument: HPLC

Analysis condition: 200~800nm 324nm A=0.02143C-0.00458, r=0.9999 0~48.672g

result: increase

Number: 4.713%

Botanical name: Hyeong-gae Application number: 10-2008-0100961. Application number: 10-2011-0124722

Scientific name: Schizonepeta tenuifolia Briquet

Latin name: Schizonepetae Spica

Identification of type: The outer surface is light yellowish green or purple, the leaves are almost absent, and the flower buds are light brown and hang at the end of the main stem. The cutting medicinal material is 3~5cm long, 0.2~0.4cm in diameter, and has a direction.

Form of Origin: Annual herb, growing in well-drained, not too fertile soil.

Plant type: It is an annual herb, and the total height is about 60cm. Branches are branched in a quadrangular stem and have short hairs. Leaves face each other and are deeply divided, and the lobes are linear with flat edges. It runs in layers on the upper part of the flower main stem and blooms in white in August-September. The corolla is shaped like a lip, and the lower lip is divided into 3 parts.

Classification: Lamiaceae Labiatae

DISTRIBUTION: Korea, China (South Central and East)

Synonyms of drugs: Hyeonggaesu(荊芥穗), Kanggae(姜芥), Kanggae(薑芥), Hyanghyeonggae(香荊芥), Regeneration(再生丹), Ilkyeopgeum(一捻金), 鄭芥, Jeonggae(假蘇) ), Seongsan Lee, Seomyeong, Geogyeonggobaesan, Eunchichae, 京芥, Gyeonggae, Seongae( ), saneunggango ( )

Scientific name: Schizonepeta tenuifolia var. japonica (Maxim.) Kitag. ; Nepeta tenuifolia Benth. ; Elsholtzia integrifolia Benth.; Glechoma japonica (Maxim.) Kuntze; Glechoma tenuifolia (Benth.) Kuntze; Nepeta japonica Maxim.; Nepeta vaniotiana H.L□v.; Schizonepeta tenuifolia (Benth.) Briq.; Schizonepeta tenuifolia var. japonica (Maxim.) Kitag.

Medicinal part:

1. Diseases of muscle and connective tissue: bone protection,

2. Symptoms, symptoms not classified elsewhere, or abnormalities found clinically or in research: anti-inflammatory, antioxidant

3. Diseases of the skin and subcutaneous tissue: antipruritic

4. Diseases of the blood and blood-producing organs, related to the immune system: antiallergic, hemostatic

Harvesting and Timing: Harvested during summer and autumn when flowers are fully bloomed and ears are green

Basic processing: Remove foreign substances and dry in the shade or in the sun with good ventilation.

How to make a cloth: Remove miscellaneous substances, use it raw for Hopoong Haepyo, and use it after burning it for hemostasis.

The present invention relates to a composition for promoting growth or preventing and improving osteoporosis, containing an extract of Schizonepetatenuifoliavar. japonica as an active ingredient. (Alkaline phosphatase) activity, it can be used as a food additive for promoting growth or preventing and improving osteoporosis by confirming its excellent bone formation increasing activity through measurement of collagen synthesis.

Botanical name: Perilla leaf, wrinkled leaflet Application number: 10-2007-0106961 Application number: 10-2019-0045432

Scientific name: Perilla frutescens Britton var. crispa decaisne

Latin Name: Perillae Folium

Classification: Lamiaceae Labiatae

State 1. Wrinkled lobules: As an annual plant, it is cultivated near fields or houses.

2. Chazugi: It is an annual plant, cultivated near fields or houses.

Botanical Name: Chazgi

Scientific name: Perilla frutescens Britton var. acuta Kudo

Latin Name: Perillae Folium

Classification: Lamiaceae Labiatae

DISTRIBUTION: 1. Early stage: Korea, China (southern), Japan 2. Pleated lobules: Korea, China, Japan

Perilla lobules discrimination:

Form of Origin: 1. Chazu period: It is an annual plant and grows up to 30~200cm in height. The stem is erect, branched a lot, purple, greenish purple or green, dull square, and has dense long soft hairs. Leaves face each other, broadly ovate, ovate circular or ovate triangular, 4-13cm long, 2.5-10cm wide, magenta or green, with hairs on both sides, but with especially long hairs on the veins on the upper surface, and sawtooth on the edge. Flowers are light purple and hang in racemes at the ends of branches and main stems in August-September.

2. Wrinkled lobules: It is an annual plant and grows straight up to 30~100cm in height. The stem is purple or purple green, round and square with many branches on the upper part. It has purple nodes and has long soft hair. The leaves are opposite, ovate or round ovate, 4-12cm long, 2.5-10cm wide, and the end protrudes or protrudes long. The base is round or wide wedge-shaped, and the edge is serrated and wrinkled. Both sides are purple, or the top side is green, and the bottom side is purple. Soft hairs are sparse on both sides, and the bottom side has thin dots. Flower racemes are terminal or axillary, slightly slanted, corolla is tube-shaped, and the tip is in the form of two labia, purple.

Drug synonyms: Susuyeop(Water蘇葉), Gong Xiaoseop(紅蘇葉), Soyeop(蘇葉), Jaso(紫蘇), Confucius Xiaoseop(紅紫蘇葉), Jeok Soseop(赤蘇葉), Sanso Sosub(山魚蘇葉), Sujang Won-seop(水壯元葉), Hyang So-seop(香蘇葉), Baek-eo So-seop(白魚蘇葉), Gye Im-seop(桂荏葉), Chuja So-seop(皺紫蘇葉)

Scientific name: Perilla frutescens var. crispa (Thunb.) H. Deane; Dentidia nankinensis Lour.; Dentidia purpurascens Pers.; Dentidia purpurea Poir.; Ocimum acutum Thunb.; Ocimum crispum Thunb.; Perilla acuta (Thunb.) Nakai; Perilla arguta Benth.; Perilla crispa (Thunb.) Tanaka; Perilla nankinensis (Lour.) Decne.; Perilla frutescens var. acuta (Thunb.) Kud@; Perilla frutescens var. arguta (Benth.) Hand. -Mazz.; Perilla frutescens var. nankinensis (Lour.) Britton

Medicinal parts: leaves, branches

1. Endocrine nutrition and circulatory system: allergy

2. Diseases of the reproductive and excretory systems: Ulcerative colitis, glomerulonephritis: 1. Scabies cell proliferation and matrix accumulation 2 degree. 1. degree of mesangial cell proliferation and matrix accumulation 2. acticity of superoxide dismutase (SOD)

3. Diseases of the circulatory system: liver protection

4. Diseases of the nervous system: sleep disorders

5. Tumor: Anticancer

Harvesting and Timing: Harvested during the summer when branches and leaves are lush

Basic processing: Remove foreign substances and dry in the shade or in the sun.

Fabric preparation method: Use after removing impurities and old age.

1. Materials

The lobules (Perilla perfrutescens var. acuta, leaf, domestic) and mint (Mentha arvensis L. var. piperascens malinvaud, leaf, domestic) used in this experiment were purchased from the Yeongcheon Herbal Medicine Wholesale Market. Leaflets and mint were packed in PP material and aluminum foil material and tested for storage at room temperature for 45 days at 15-day intervals.

2. Method

㉮ Moisture measurement

Each sample was taken regularly and measured according to the normal pressure drying method. That is, after obtaining the constant weight of the weighing bottle, the sample was put in, heated in a dry oven at 105 ° C. for 2-3 hours, cooled in a desiccator for 30 minutes, and then the weight of the weighing bottle was measured. If the error from the first measurement was within 0.2 mg, the constant weight was reached.

㉯ color difference

The measurement of chromaticity is an important factor in stimulating the consumer's palatability. Herbal medicine is pulverized, diluted in water and extracted, and measured using a color meter (Minolta, CR-300, Japan), which is Hunter value, that is, lightness (L), Indicated by redness (a) and yellowness (d)

㉰ Total number of bacteria

The degree of microbial growth during storage of herbal medicines was confirmed by measuring the total number of bacteria. Put 10 g of the sample in a sterile cup, add 90 mL of sterile water, homogenize at 5,000 rpm for 5 minutes with a homogenizer, and spread 0.1 mL of the dilution in 10-fold step-by-step on a plate count agar (PCA, Difco) prepared in advance. After inoculation, colonies were counted and confirmed after incubation at 25 ° C for 48 hours, and the number of detected microorganisms was expressed as log colony forming unit (Log CFU / g) per 1 g of sample.

㉱ Extracts content measurement

Precisely weigh 2.3 g of herbal medicines in 70 ml of dilute ethanol, put them in a suitable flask, shake occasionally, and infuse for 5 hours. Leave for another 16-20 hours and then filter. The flask and residue are washed with dilute ethanol until the filtrate is 100 ml. 50 ml of the filtrate is evaporated to dryness on a water bath, dried at 105°C for 4 hours, cooled in a desiccator (silica gel), weighed accurately, and multiplied by 2 to obtain the amount of dilute ethanol extract. Calculate the X content (%) for the amount of sample converted to dry matter from the value obtained from the loss on drying

㉲ sensory test

In the sensory test, the appearance and aroma were evaluated while storing the herbal medicine at room temperature to find out the changes that occurred during the storage period (0, 15, 30, and 45 days) of the herbal medicine. The selected sensory agents consisted of 16 people who were considered to have the ability to discern the difference in quality of herbal medicines through sufficient training. The evaluation method was carried out by the preference test method with a 5-point method. Very bad: 1 point, bad: 2 points, average: 3 points, good: 4 points, very good: 5 points, and each sample was evaluated.

㉳ Statistical processing

Statistical processing was expressed as mean ± standard error for each sample, and the significant difference according to each group was analyzed according to Duncan's multiple test at α = 0.05 level after performing variance analysis.

3. Results and discussion

㉮ Moisture

The results of measuring the change in moisture content at 15-day intervals while packing mint and leaflets, which are aromatic herbal medicines, in PP and aluminum foil materials and storing them at room temperature for 45 days are as follows. As for the change in moisture content during storage, the aluminum foil packaging generally maintained a higher moisture content than the current PP packaging (Fig. 1). However, the difference in moisture content was not large, and the extent of decrease during storage was smaller in aluminum foil packaging than in current PP packaging. Therefore, it is considered that the change in moisture content can be minimized when packaging with aluminum foil. The moisture content of the aluminum foil was 8.19% for mint and 8.54% for leaflets on the 45th day of storage, which was only a difference of 0.1-0.2% from the initial moisture content. Among the storage stability studies by packaging material, Choi et al.13) measured the change in water activity of beef jerky according to the packaging method. As a result, vacuum packaging showed higher water activity than normal packaging from 45 days and 75 days of storage, depending on the sample. In all treatment groups, the water activity of the general packaging tended to decrease as the storage period elapsed (p < 0.05), but the vacuum packaging did not show any change in water activity as the storage period elapsed.

Fig. 1. Comparative effects of packaging material (aluminium, PP) on the moisture content of Mentha arvensis L. var. piperascens malinvaud. and Perilla frutescens during storage in room temperature. PM: PP package (Mentha arvensis L. var. piperascens malinvaud.), PP: PP package (Perilla perfrutescens var. acuta), AM: Aluminum package (Mentha arvensis L. var. piperascens malinvaud.), AP: Aluminum package

㉯ color difference

The change in color difference during storage of mint and lobules, which are aromatic herbal medicines, tended to decrease in L value during storage, but aluminum foil packaging tended to have a slightly higher L value overall than current PP packaging (Fig. 2-4) . However, overall, mint showed a higher L value than leaflet. During storage, the color difference a value tended to increase, but the b value did not change significantly until 30 days of storage, and thereafter tended to decrease slightly. Park et al. 14) compared and reviewed the quality change while immersing lotus root in various immersion liquids, then wrapping and vacuum-packing it and storing it at 5 ° C for 35 days. and b values showed a gradual increase, and it was reported that the range of change was smaller in vacuum packaging than in lap packaging.

Fig. 2. Comparative effects of packaging material (aluminium, PP) on the hunter(L) of Mentha arvensis L. var. piperascens malinvaud and Perilla perfrutescens var. acuta during storage in room temperature Simbols are the same as those in Fig. One.

Fig. 3. Comparative effects of packaging material (aluminium, PP) on the hunter(a) of Mentha arvensis L. var. piperascens malinvaud and Perilla perfrutescens var. acuta during storage in room temperature Simbols are the same as those in Fig. One.

Fig. 4. Comparative effects of packaging material (aluminium, PP) on the hunter(b) of Mentha arvensis L. var. piperascens malinvaud and Perilla perfrutescens var. acuta during storage in room temperature Simbols are the same as those in Fig. One.

㉰ Total number of bacteria

As for the change in the total bacterial count during storage of aromatic herbal medicines mint and leaflet, the aluminum foil packaging showed a slightly lower total bacterial count than the current PP packaging, and the mint herb showed a lower total bacterial count than the leaflet (Fig. 5). It seems that mint itself has antibacterial properties rather than leafy herbal medicines, and it is judged that aluminum foil packaging can minimize contamination by microorganisms. Yuk et al. [15] also reported that gamma irradiation can significantly reduce contaminating microorganisms in herbal medicines. As a study supporting this, Park et al.14) compared and reviewed the quality change for 35 days for wrap packaging and vacuum packaging, and found that wrap and vacuum packaging showed a relatively low total number of bacteria at 1 log cycle compared to the initial total number of bacteria. did This difference was maintained at a slightly lower level than the control until 15 days of storage in the lab packaging and 28-35 days in the vacuum packaging. Therefore, it was reported that vacuum packaging was more effective in inhibiting microbial growth than wrap packaging. Choi et al.13) measured the change in total bacterial count during storage of reconstituted stick-type jerky according to the packaging method and reported that vacuum-packed jerky during storage showed a lower total bacterial count than normal packaging.

Fig. 5. Comparative effects of packaging material (aluminium, PP) on the microbial growth of Mentha arvensis L. var. piperascens malinvaud and Perilla perfrutescens var. acuta during storage in room temperature Simbols are the same as those in Fig. One.

㉱ Extracts content

There was little difference in the extraction rate between the aluminum foil packaging and the current PP packaging for the change in extracts content during storage of mint and leaflets, which are aromatic herbal medicines, and overall, mint extract was much higher than leaflet (Fig. 6). At the same time, the change in extraction rate during storage was almost insignificant. This is consistent with the report of Yuk et al. [15] that herbal medicine extract tends to increase by gamma ray irradiation.

Fig, 6. Comparative effects of packaging material (aluminium, PP) on the extractability of Mentha arvensis L. var. piperascens malinvaud and Perilla perfrutescens var. acuta during storage in room temperature Simbols are the same as those in Fig. One.

㉲ sensory test

As a result of the sensory test result during the storage of mint and leaf, which are aromatic herbal medicines, there was almost no change in appearance, but as a result of the evaluation of fragrance, the aluminum foil packaging showed a higher evaluation than the current PP packaging (Fig. 7, 8). It is considered that aluminum foil packaging is a good packaging material for maintaining the fragrance during storage of mint and lobules, which are aromatic herbal medicines. During a field study in Japan, silver foil packaging is used to package aromatic herbal medicines in Japan, which supports these results.

Fig. 7. Comparative effects of packaging material (aluminium, PP) on the appearance of Mentha arvensis L. var. piperascens malinvaud and Perilla perfrutescens var. acuta by sensory evaluation during storage in room temperature

Simbols are the same as those in Fig. One.

Fig. 8. Comparative effects of packaging material (aluminium, PP) on the flavor of Mentha arvensis L. var. piperascens malinvaud and Perilla perfrutescens var. acuta by sensory evaluation during storage in room temperature

Simbols are the same as those in Fig. One.

conclusion

The results of measuring the change at 15-day intervals while packing mint and leaflets, which are aromatic herbal medicines, in PP and aluminum foil materials and storing them at room temperature for 45 days are as follows. As for the change in moisture content during storage, the aluminum foil packaging generally maintained a higher moisture content than the current PP packaging. As for the change in the total bacterial count during storage of aromatic herbal medicines mint and leaflets, the aluminum foil packaging showed a slightly lower total bacterial count than the current PP packaging, and the total bacterial count of mint herbal medicine was lower than that of the leaflets. As a result of the sensory test of mint and lobules, which are aromatic herbal medicines, there was almost no change in appearance, but as a result of the evaluation of fragrance, the aluminum foil packaging showed a higher evaluation than the current PP packaging. Considering this point, it is judged that aluminum foil packaging is applied to herbal medicines (leaves, peppermint, etc.) with fragrance in the future, and a packaging method using a zipper bag is preferable to prevent the loss of fragrance when a small amount of herbal medicine is used. . Through field research and literature research in Japan and China, oriental medicines with strong aromatic properties are mainly used in airtight and flavor-preserving silver foil (aluminum foil) packaging to prevent loss of medicinal effects.

lobules, ethyl acetate, fractions, cytotoxicity

In order to investigate the effect of ethyl acetate 5 fractions of lobules on the cytotoxicity of human oral epithelial carcinoma cells, each fraction was treated with human oral epithelial carcinoma cells, and then the cell number was calculated using a hemocytometer and MTT was measured and inverted. As a result of observing the formazan crystal shape of cells under a microscope, the following conclusions were obtained. 1. Ethyl acetate subfraction 1 of lobules showed cytotoxicity only at the concentration of 10 μg/ml, and showed the strongest cytotoxicity with statistical significance (p<0.001), with a cell viability rate of 70.3% of the control group. 2. Comparison of the 50% inhibitory effect of the ethyl acetate fractions of lobules on human oral epithelioid carcinoma cells showed that the cytotoxicity increased in the following order. Small Fraction 5 (MTT50 = 73 μg/mL) < Small Fraction 4 (MTT50 = 29 μg/mL) < Small Fraction 3 (MTT50 = 27 μg/mL) < Small Fraction 2 (MTT50 = 20 μg/mL) < Small Fraction 1 (MTT50 = 17 μg/ml). When the change in cell number according to the ethyl acetate fraction of the lobules and the cell viability by MTT measurement were combined, the cytotoxic effect of human oral epithelioid carcinoma cells was the strongest in subfraction 1.

Perilla frutescens (P. frutescens) is an important herb used in Asian countries for many purposes such as medicinal, aromatic and nutraceutical, and has beneficial effects such as antioxidant activity, anti-inflammatory activity, anti-depressive activity and anxiolytic activity. However, there have been no studies on the protective effects of P. frutescens extract (PFE) on memory loss in vivo. The present study aimed to investigate whether PFE protects against memory deficits using a scopolamine-induced mouse model and elucidates the underlying mechanisms involved. The protective effect of PFE against scopolamine-induced memory deficits was investigated using the Y-maze, passive avoidance, and Morris water maze tests. Moreover, the potential mechanisms of PFE to enhance memory function related to the cholinergic system and antioxidant activity were examined. PFE significantly increased spontaneous alternation in the Y-maze test, step-by-step latency in the passive avoidance test, and swimming time in the target quadrant of the probe test compared to the scopolamine-treated group. Similarly, PFE significantly reduced latency in the Morris water maze test. PFE cannot modulate cholinergic function in acetylcholine levels and acetylcholine esterase activity. However, PFE dose-dependently increased DPPH radical scavenging activity and the total polyphenol content was 127.7±1.2 μg GAE/mg. Results showed that PFE could be a prophylactic and/or therapeutic candidate for memory and cognitive dysfunction in Alzheimer's disease.

The present invention relates to a method for producing perilla leaf pigment using perilla leaf extract, and the pigment is currently used in various ways as an additive or auxiliary agent ranging from food industry, cosmetics, pharmaceuticals and livestock feed. In particular, the color of a product plays an important role in determining its marketability. Accordingly, many studies and efforts are being made to apply natural food pigments to more diverse products. The present invention comprises the steps of (A) collecting unfaded perilla leaves around May-August and then washing them, (B) dehydrating the washed perilla leaves with a centrifuge, and (C) using a centrifuge for Perilla leaf dehydrated by perilla leaf is mixed with sugar in an amount equal to the weight of perilla leaf as an extraction solvent, and then glassed.

Botanical Name: Black Book Sundew, (蟬退), Cicadidae Periostracum

Application number: 10-2016-0038979 Application number: 10-2004-0074799

Scientific name: Cryptotympana pustulata Fabricius

Latin Name: Cicadidae Periostracum

Classification: Cicada Cicadidae

State 1. Sunte:

2. Horse cicada:

Botanical Name: Horse cicada

Scientific name: Cryptotympana dubia (Haupt)

Latin Name: Cicadidae Periostracum

Classification: Cicada Cicadidae

Distribution: 1. Selection:

Selective diagnosis:

2. Selection:

Synonyms of drugs: Seon-gak (蟬殼), Go-seon (枯蟬), Seon-gap (蟬甲), Jetoe-gak (蟬退殼), Seon-tae (蟬退), Seon-ui (蟬衣)

Scientific name: none

Medicinal part: skin

1. Endocrine nutrition and circulatory system: hyperlipidemia The isolated compounds inhibited lipid and oxidation in TBARS assay and inhibited NO and ROS production, NF-kB, iNOS, IL-6, TNF-alpha, and COX-2 expression in LPS-treated RAW264.7 cells.

2. Symptoms, conditions not classified elsewhere, or abnormalities found clinically or in research: antioxidant, anti-inflammatory, anti-inflammatory Reduction of NO production by LPS

3. Injury, poisoning and those from external causes: 1. Acute toxicity As a result of oral administration of Xiaoer Chaigui Tuire oral liquid, the maximum-tolerated does in mice was 300g/kg, and the NOAEL was 20-80g/kg after 30 days of administration.

4. Anti-allergy Conalbumin-induced mouse analyphylaxis was inhibited by Guo Min Kang

㉮ Diseases of the reproductive and excretory systems: glomerulonephritis Inhibition of damage caused by glomerulonephritis when Qi Teng Xiao Zhuo granules are given to rats

㉯ Diseases of the digestive system: liver cancer Investigated as a drug used for liver cancer in a survey of the elderly population in the local community

㉰ Diseases of the circulatory system: ㉠, cerebral infarction Promote neurogenesis and angiogenesis for cerebral infarction in stroke-prone renovascular hypertensive rats

㉡ antithrombotic Cryptonin, an isolated peptide, exhibits hemolytic action

4. Infectious disease or parasitic disease: antimicrobial The isolated peptide, cryptonin, shows antimicrobial activity against G(+), G(-) bacteria and fungi, including methicilin-resistant Staphylococcus aureus and vancomycin-resistant Enterococci.

5. Diseases of the skin and subcutaneous tissue

㉠ psoriasis It is known to inhibit HaCaT cell proliferation, but the IC50 was not good in this experiment.

hives Investigate Taiwan's national chinese herbal medicine (CHM) prescription DB to identify the most frequently used for hives

Urticaria, atopic dermatitis Taiwanese National Health Insurance Research Database confirms that sundew is frequently prescribed for urticaria and atopic dermatitis

6. Collection and timing: Collect in summer and autumn

Basic processing: Shake off the soil and dry it in the sun.

Preparation method: Take the raw material, remove impurities, wash thoroughly, and dry.

Characteristics of Pollination: Seontae ( ) is slightly oval and curved, about 3.5 cm long and about 2 cm wide. The surface is yellowish brown, translucent and glossy. There is a pair of thread-like tentacles on the head, but most of them have fallen off, and two eyes protrude from the side. The tip of the chin protrudes, the snout is developed, the upper lip is wide and short, and the lower lip is long and tubular. The thorax and abdomen are divided in a cross shape and are rolled inside toward the fissure, and there are two pairs of small collars on both sides of the back. There are three pairs of legs on the abdomen, which are covered with yellowish-brown fine hair. The abdomen is a dull circle with nine segments. The body is light and hollow, so it is easily broken, there is no smell, and the taste is light.

Poje Purpose: Sexual cold, maegam (味甘), mouth lung liver disease (入肺肝經). Dissipation Wind Fever·Sukjin Retardation·Retirement of Honor( ) Geopungjigyeong (祛風止痙) → Chichigamukpungyeol (感冒風熱), heat pain (咽痛), yin and yang (sound 啞), marginal abstinence (痲疹不透), rubella soreness (wind塵瘙痒), purpose etiquette ( ), conjecture guess ( ), tetanus, etc.

After going through the process of removing impurities and washing, the material of the drug becomes clean.

The present invention relates to a novel acetyldopamine-based compound and a composition for the prevention and treatment of cardiovascular diseases containing as an active ingredient a novel acetyldopamine-based compound and an acetyldopamine-based compound isolated from a Phiji extract or an acetyldopamine-based compound isolated therefrom. Since the acetyldopamine-based compound has excellent antioxidant activity against low-density lipoprotein, a composition containing it can be usefully used for the prevention and treatment of cardiovascular diseases such as hyperlipidemia or atherosclerosis caused by oxidation of low-density lipoprotein. .

Gumac, (瞿麥), Dianthi Herba

Application number: 10-2018-0059728 Application number: 10-2012-0027791

1, Botanical name: dianthus

Scientific name: Dianthus chinensis Linn□

Latin name: Dianthi Herba

Classification: Caryophyllaceae

Status : Dianthus : Dianthus : It is a perennial plant with root-bearing properties. It grows well in dry places in lowlands, barren grasslands, sandy areas along streams and forest edges. I like sunny places.

Botanical Name: 2, Sulfur Dianthus: It is a lahaesalyi grass. It grows in dry, barren and exposed places on grass or at the foot of a mountain.

Scientific name: Dianthus superbus var. longicalycinus Williams

Latin name: Dianthi Herba

Classification: Caryophyllaceae

Distribution : Dianthus : Korea, China, Russia, Mongolia, Kazakhstan, Europe Dianthus : Korea, Japan, China

Differentiation: consists of stem, branch, leaf, and inflorescence. The stem is columnar, glossy, and the nodes are distinct. It is 30 to 40 cm long and has a pale green or yellowish green color. The cut side is hollow inside. The leaves are wrinkled and linear to linear lanceolate. The flowers are 3-4 cm long, the tip of the corolla is deeply split, and it is light purple. The length of the calyx tube is about 3/4 of the whole flower, and the length of the sepal is about 1/4 of the calyx tube.

Shape: 1, Dianthus: It is a perennial herb, the total height is about 30cm, and the whole is pinkish white. Leaves are linear or lanceolate, the edges are flat, and the lower parts of the opposite leaves are joined together to cover the stem. It hangs at the end of the flower stem and blooms one by one in June-August. The calyx is cylindrical, 2 cm long, and the tip is split into 5 pieces. The petals are 5, spreading sideways, the edges are shallowly split, and there are 10 stamens and 2 styles. The fruit capsule is divided into 4 pieces at the end.

2, Sulfur dianthus: It is a perennial herb with a total height of 30 to 100 cm and a white color throughout. The lower part of the stem grows obliquely and branches, and the upper part grows straight. The leaf is linear or lanceolate, the edge is flat, and the lower part of the opposite leaf is joined together to surround the node. It hangs at the end of the flower branch and the main stem, and blooms in light red in July-August. The length of the calyx tube is 2.5~4cm, and the petals are 5, and the tip is deeply split. There are 10 stamens and 2 styles. Fruit capsules are columnar and split into 4 ends.

3. Symptoms, symptoms not classified elsewhere, or abnormalities found clinically or in research: 1, antioxidant Shows some scavenging effect against DPPH free radical and hydroxy radical and weakly inhibits lipid peroxidation of rat brain homogenate

2, DPPH free radical scavenging efficacy

4. Injury, poisoning and those caused by external causes: analgesia Mouse ( ) ,1,Acetic acid is injected by ip and shows analgesic effect when writhing test 2,Mouse ( ) ,Acetic acid was injected ip and showed analgesic effect when writhing test was performed

5. Diseases of the digestive system: liver protection Hepatocyte protection against primary cultured rat hepatocytes, CC14 and galactosamine-induced hepatotoxicity

9. Infectious disease or parasitic disease: antibacterial action Inhibiting the growth of Propionibacterium acnes, an acne-causing bacterium

10. Mental and Behavioral Disorders: Cognitive Function Cognitive function was recovered in the passive avoidance test and the Morris water maze test of mice and Scopolamine-administered mice. In addition, it was confirmed that it inhibits the activity of acetylcholinesterase in Hippocampus and increases BDNF.

11. Tumor: Anticancer effect, ㉮, HepG2 cell, inhibiting cancer cell line proliferation, inducing apoptosis by regulating caspase, bax, and bcl ㉯, HepG2 cell, among the isolated compounds, longicalycinin A shows cytotoxicity in HepG2 ㉰, SCC-15 cells, human normal oral mucosa, human oral squamous cell carcinoma, inhibition of cancer cell proliferation, reduced Sp1 expression to show apoptotic effect

㉱, H1299 human lung carcinoma cell, HCT116 human colorectal carcinoma cell, suppress cell proliferation and cell adhesion

Harvesting and timing: Collected during flowering between summer and autumn

Basic processing: Remove foreign substances and dry in the shade.

Fabric preparation method: Remove impurities, cut into small pieces, and use raw. Geogumaek (乾瞿麥): Wash the goumaek (瞿麥) well, and when moisture permeates, cut it and dry it in the sun. When using it, soak it in bamboo charcoal for a day and night, then take it out and dry it in the sun.

It relates to a composition for the prevention or treatment of inflammatory diseases comprising an extract of sagebrush as an active ingredient. The sagebrush extract of the present invention exhibits antioxidant, NO production inhibition, and iNOS protein expression inhibitory effects, and in animal experiments, edema, inflammatory cell infiltration, and myeloperoxidase (Myeloperoxidase) activity is inhibited to show excellent anti-inflammatory effect, so it can be used as a pharmaceutical composition and cosmetic composition to suppress inflammatory diseases.

Inhibitory effect on clumped glomeruli of Alismaorientale

Application number: 10-2000-0078052 Application number: 10-2012-0050730

I. Introduction

The lumpy root of Chamtaeksa or plantain taeksa, a perennial root plant belonging to the family of Taeksa, is used for medicinal purposes.

It is salted, stir-fried, or dipped in sake.

Form: Plantain: It is a perennial plant, the height to the stem is about 50cm, and it is generally hairless. There is a root beard, there is no main stem, and many leaves come out from the root and spread obliquely. Leaves are ovate or elliptical, the edge is wavy, and usually has 5 to 7 arc-shaped veins. The tip is pointed or the base is narrowed to become a long petiole and is dull.

The long petiole is almost the same length as the leaf blade or longer than the leaf blade, and the lower part is widened to embrace each other. The flowers are light green, and the spikelets are 2/5~1/2 of the scape. There are several scapes with a height of 12 to 50 cm, with rhomboids and sparse hairs. When the fruit capsule is ripe, it splits to the side, and the upper half is conical ovate, and 6 to 8 oval dark brown seeds come out.

Hairy Jinggyeongi: This is a perennial herb with coarse hairs throughout. The root is a columnar rhizome. Leaves are long oval or elliptical lanceolate, and the base is narrow. Flower spikes are 2/5~1/2 of the peduncle, and the flowers are light green. It is a fruit capsule and has a conical shape, and the seed is different from the chaejeon in that it is a long oval shape.

Hairy gyeonggi: It is flat and oblong, but some are close to triangular, and the body is relatively small. It is about 1 to 1.8 mm long and about 0.6 to 1 mm wide. The outer surface is dark brown or brown, the back is slightly raised, the lower part is relatively flat, and there is a white, concave, dot-shaped terminal in the center.

1} Until now, there have been various reports about the ingredients contained in plantain taxa. Representative ingredients include diuretic action, skin relief action, cholesterol lowering action, edema, beriberi, diabetes, dizziness, glandular fever, clearing fire, hyperlipidemia, prostatitis, vomiting, nephritis, hypertension, fever. , aucubin, geniposidic acid, etc. as iridoidal glycosides, plantagoside as flavonoidal glycosides, and actio as phenyl ethanoid glycosides. side plantain (acteosideplantain joside), etc.

Representative physiological activities of psyllium and plants of its genus have been in progress for a long time in various fields. In the immunomodulatory activities test, a lot of substances isolated from Plantago were classified into a total of 5 compound groups, and a total of 12 substances were classified into human PBMC peripheral blood moiu nuclear cells. As a result of experiments on the activity of regulating the differentiation of nuclear cells and the secretion of IFN-y, except for luteolin, aucubin, chlorogenic acid, and ferulic acid (chlorogenic acid, ferulic acid, p-coumaric acid) ) showed immunomodulatory activity, while the triterpenoids oleanolic acid and ursolic acid did not play a role as potential modulators in lymphocyte differentiation, but stimulated the secretion of IFN-Y from the lymphocytes.

In addition, neutral arabinoxylans in polysaccharides isolated from the water extract of the seeds of P. ovata significantly increased the differentiation rate of HaCaT keratinocytes, and P. 'm. There have also been reports of promoting cell differentiation in the aerial part of or. P. n] The promotion of cell differentiation in the aerial part of jor was evaluated by comparing the hematopoietic activity in the bone marrow and spleen of rats. As a result of comparing the activities of the aqueous layer and the MeOH layer in the bone marrow and spleen of rats, the activity of both layers was evident, and especially in the bone marrow, the MeOH layer showed a much more excellent effect than the aqueous layer at the concentrations of 0.1 and 0.05 g/mL.

According to a report on the evaluation of antioxidant activity in Plantogo, MeOH extracts were found to be effective against DPPH diphenyl picrylhydrazyl and Fe2+/ascorbate-derived LPO using bovine brain liposomes. Ascorbate-induced LPO It has been reported that there is an excellent antioxidant effect in an ascorbate-induced LPO inhibition assay. And it has been reported that flavonoids, PPG phenylpropanoids, which are abundant in Plantago, Content and scavenging of phenyl propanoid glycosides and iridoids and inhibition of Fe2+/ascorbate-induced LPO DPPH diphenyl picrylhy drazyl ) was derived in a mutually dependent manner.Reports on the antioxidant activity of geniposidic acid as a representative iridoid glycoside isolated from psyllium include oxidation of linoleic acid. As a result of evaluating antioxidant activity by an assay based on , geniposidic acid is weaker than BHA (butylated hydroxyanisole) and BHT butylated hydroxytoluene, which have been used as existing antioxidants, but , dl-(2 -tocopherol) showed the possibility of development as an antioxidant by showing stronger activity than tocopherol》

Among them, the enzyme tyrosinase is involved in the process from tyrosine to dopaquinone, and after that, it is said that the contribution of autoxidation is large. In other words, it effectively suppresses the action of tyrosinase involved in the process of hydroxylating tyrosine to produce dopa and the process of oxidizing dopa to produce dopaquinone. May inhibit melanin biosynthesis. In addition to tyrosinase, factors that promote or inhibit melanin production, such as dopachrome conversion factor and blocking factor of tyrosinase, prostaglandin (PG), -interferon ) (INF), many melanocyte stimulating hormone (MSH), MSH receptor activity, vitamin D3, hiifemin, etc. are known to be involved in melanin pigment production.

Recently, it has been found that melanin production is not only oxidized by tyrosinase but also by various complex factors, and in vitro tyrosinase activity inhibitors have been shown to be effective in melanoma cell lines ( Due to problems such as the absence of activity in melanoma cell lines), the development of a substance that comprehensively inhibits melanin production is required.

Harvesting and timing: Harvested in winter when stems and leaves begin to wither

Basic processing: Trim the stems and small roots, wash in water, trim the outer skin with a knife, and dry in the sun.

Fabrication method: Pick out impurities from the raw material, classify them according to size, soak them in clear water, and when about 70% of the moisture is absorbed, wash them clean, put them in the shade, and seal them for 12 to 24 hours. Cut into half.

Yeomtaksa (鹽澤瀉): After spraying a certain amount of saline solution on a clean taxidermy piece and moistening it evenly, ) Put it in a container and heat it to the point where it turns slightly yellowish in color and let it cool in the shade. kg).

Bucho Taxi ( ): pulse ( ) to the four ( ) Put it in a container, heat it with neutral fire, and when thick smoke comes out, add a piece of taxicab and turn it over continuously, take it out when the surface turns yellow, and sift it ( ) and leave to cool in a cool place. At this time, the point to be careful is to use dried slices, but if not, it is easily bulky ( ) and sieved to the volume ( ) becomes difficult to remove (volume per 100 kg ) 10 kg).

Porous appearance: Circular oblate, about 3mm thick, about 2.5-4.5cm in diameter, the surface is yellowish white and flat, with sponge-shaped small holes and longitudinally and transversely scattered muscle veins. ) pattern, and the edge has traces of protruding dense beard roots in yellowish white. The quality is hard, the smell is weak, and the taste is slightly bitter.

Yeomtaeksa (鹽澤瀉): The shape is the same as a taxidermy raw piece (澤瀉生片), and the surface is light yellowish color (yellow fire color), sometimes with yellow spots (黃焦斑). The taste is slightly sweet.

Bucho Taxi ( ) : The surface is yellow, and the initial (焦斑) is sometimes seen, and there is a slight scent of vinegar (焦香气). The taste is slightly bitter.

Poje Purpose: Sexual cold, taste, and bladder. Urinary incontinence, heat in dampness → dysuria, dysuria, dizziness, urea in diarrhea, phlegm, fever Intestinal pain and hyperlipidemia, etc.

Raw material: Isu Samseup ( ), acting on the disease, urinary incontinence, dropsy, damp-heat jaundice, damp-heat reflux, impatience, diarrhea, phlegm (痰) It is widely applied to symptoms such as

鹽水製: It has the property of descending, so the action of 滋陰, 泄瀉, and 利水 is enhanced, and it also enhances lucidity but does not harm. Urinary shovel ( ), Sujeong Im ( ) , lumbar pain, yoseul acidity ( ) is mainly used for symptoms such as

Bucho ( ): Coldness is alleviated ) and hangtak (降濁) to make it seungcheong (昇淸), which is mainly used to make seungcheong (昇淸), such as Biseubyesa (脾濕泄瀉), Damseupgyeonun (痰濕眩暈), Biheoyesa (脾虛泄瀉), Gusa (久瀉). used for symptoms such as

Experiment details

Test target: alisol B23-acetate

Packaging Method: 1. After dissolving an appropriate amount of table salt in water, dip the taxicab into brine, put it in a pot, fry it in culture, take it out and cool it. 2kg of table salt per 100kg package

Solvent: Methanol

Analytical instrument: HPLC

Analysis conditions: y=21536x+3.675, r=1.000, 0.05~2.51μg

Number: 0.001338 Result: None

Test target: alisol B23-acetate

How to make: 2. Put the taxidermy in a container, make a saline solution with salt and boiling water, sprinkle it on the taxidermy, soak it for 4-6 hours, and when it is all absorbed, take it out and dry it in the sun. Place the wheat bran in a pot, stir-fry it with taxicab, take it out and cool it.

Solvent: Methanol

Analytical instrument: HPLC

Analysis conditions: y=21536x+3.675, r=1.000, 0.05~2.51μg

Number: 0.001291 Result: None

3. Experiment target: alisol B23-acetate

Method of packing: 3. Put saline solution in a taxiderm and stir-fry in weak water. When the surface turns yellow, take it out and cool it. Dissolve 2kg of table salt in 100kg of rice and 6kg of water.

Solvent: Methanol

Analytical instrument: HPLC

Analysis conditions: y=21536x+3.675, r=1.000, 0.05~2.51μg

Number: 0.001425 Result: None

Test target: alisol B23-acetate

Method of preparation: 4. Add taxicab to alcohol, mix, and when absorbed, stir-fry in culture and cool. 10kg yellow wine in 100kg courier

Solvent: Methanol

Analytical instrument: HPLC

Analysis conditions: y=21536x+3.675, r=1.000, 0.05~2.51μg

Numerical value:0.001388 Result: none

Test target: alisol B23-acetate

Wrapping method: 5. Dip the silk in saline and dry it in an oven at 60 degrees for 1 hour

Solvent: Methanol

Analytical instrument: HPLC

Analysis conditions: y=21536x+3.675, r=1.000, 0.05~2.51μg

Number: 0.001465 Result: None

Test target: alisol B23-acetate

How to wrap: 6. Tie in a 160 degree pot for 6 minutes

Solvent: Methanol

Analytical instrument: HPLC

Analysis conditions: y=21536x+3.675, r=1.000, 0.05~2.51μg

Number: 0.00137 Result: None

Test target: alisol B23-acetate

Poaching method: 7. Put malt in a pot and stir-fry it. When smoke comes out, add rice flour and stir-fry.

Solvent: Methanol

Analytical instrument: HPLC

Analysis conditions: y=21536x+3.675, r=1.000, 0.05~2.51μg

Number: 0.001565 Result: None

Test target: alisol B23-acetate

Fabric preparation method: 8. Soak silk in saline solution overnight and then dry. After heating the pot, put the malts and when the smoke comes out, add taxicab and stir-fry it, take it out when it turns golden yellow, and filter the malts.

Solvent: Methanol

Analytical instrument: HPLC

Analysis conditions: y=21536x+3.675, r=1.000, 0.05~2.51μg

Number: 0.001408 Result: None

Test target: alisol B23-acetate

Wrapping method:

Solvent: acetonitrile

Analytical instrument: HPLC

Analysis conditions: y=21536x+3.675, r=1.000, 0.05~2.51μg

Number: 0.00155 Result: Increase

Test target: A 24-acetate

Wrapping method: none

Solvent: acetonitrile

Analytical instrument: HPLC

Analysis conditions: y=798.67x+0.6438, 0.9998, 0.8~0.05μg

Numerical value: 0.013±0.004 Result: Standard

Test target: A 24-acetate

Wrapping method: none

Solvent: acetonitrile

Analytical instrument: HPLC

Analysis conditions: y=798.67x+0.6438, 0.9998, 0.8~0.05μg

Value: 0.013±0.004→0.020±0.004 Result: increase

Test target: A 24-acetate

Wrapping method: none

Solvent: acetonitrile

Analytical instrument: HPLC

Analysis conditions: y=798.67x+0.6438, 0.9998, 0.8~0.05μg

Value: 0.013±0.004→0.032±0.010 Result: Increase

Test target: A 24-acetate

Wrapping method: none

Solvent: acetonitrile

Analytical instrument: HPLC

Analysis conditions: y=798.67x+0.6438, 0.9998, 0.8~0.05μg

Value: 0.013±0.004→0.024±0.005 Result: Increase

Test target: A 24-acetate

Wrapping method: none

Solvent: acetonitrile

Analytical instrument: HPLC

Analysis conditions: y=798.67x+0.6438, 0.9998, 0.8~0.05μg

Numerical value: 0.091±0.008 Result: standard

Test target: A 24-acetate

Wrapping method: none

Solvent: acetonitrile

Analytical instrument: HPLC

Analysis conditions: y=798.67x+0.6438, 0.9998, 0.8~0.05μg

Numerical value: 0.091±0.008→0.077±0.009 Result: decrease

Test target: A 24-acetate

Wrapping method: none

Solvent: acetonitrile

Analytical instrument: HPLC

Analysis conditions: y=798.67x+0.6438, 0.9998, 0.8~0.05μg

Numerical value: 0.091±0.008→0.028±0.005 Result: decrease

Test target: A 24-acetate

Wrapping method: none

Solvent: acetonitrile

Analytical instrument: HPLC

Analysis conditions: y=798.67x+0.6438, 0.9998, 0.8~0.05μg

Numerical value: 0.091±0.008→0.033±0.003 Result: decrease

Experiment target: alisol A

Wrapping method: none

Solvent: acetonitrile

Analytical instrument: HPLC

Analysis conditions: y=868.45x-0.5042, r=0.9998, 0.8~0.05μg

Number: Result: Criterion

Experiment target: alisol A

Wrapping method: none

Solvent: acetonitrile

Analytical instrument: HPLC

Analysis conditions: y=868.45x-0.5042, r=0.9998, 0.8~0.05μg

Number: Result: Criterion

Experiment target: alisol A

Wrapping method: none

Solvent: acetonitrile

Analytical instrument: HPLC

Analysis conditions: y=868.45x-0.5042, r=0.9998, 0.8~0.05μg

Numerical value: -→0.042±0.005 Result: increase

Experiment target: alisol A

Wrapping method: none

Solvent: acetonitrile

Analytical instrument: HPLC

Analysis conditions: y=868.45x-0.5042, r=0.9998, 0.8~0.05μg

Numerical value: -→0.041±0.003 Result: increase

Experiment target: alisol A

Wrapping method: none

Solvent: acetonitrile

Analytical instrument: HPLC

Analysis conditions: y=868.45x-0.5042, r=0.9998, 0.8~0.05μg

Number : Result: Criterion

Experiment target: alisol A

Wrapping method: none

Solvent: acetonitrile

Analytical instrument: HPLC

Analysis conditions: y=868.45x-0.5042, r=0.9998, 0.8~0.05μg

Value: -→0.002 Result: Increase

Experiment target: alisol A

Wrapping method: none

Solvent: acetonitrile

Analytical instrument: HPLC

Analysis conditions: y=868.45x-0.5042, r=0.9998, 0.8~0.05μg

Value: -→0.006±0.003 Result: Increase

Experiment target: alisol A

Wrapping method: none

Solvent: acetonitrile

Analytical instrument: HPLC

Analysis conditions: y=868.45x-0.5042, r=0.9998, 0.8~0.05μg

Value: -→0.003±0.001 Result: Increase

1. Code for special purposes

Biological activity model: In vitro RAW264.7cell evaluation index: NO↓

Antioxidant effect model: In vitro HepG2 evaluation index: ROS ↓

2. Diseases of the skin and subcutaneous tissue

Atopic dermatitis model: In vitro, Rat evaluation index: 5-lipoxygenase (5-LOX)-catalyzed leukotriene↓, β- hexosaminidase↓

3. Diseases of the respiratory system

Lung disease: Model: In vitro, RAW264.7cell Evaluation index: NF-kB↓, COX-2↓, IL-1 β ↓, iNOS↓

: Model : In vivo, mice Evaluation index : LC3-II↓

Normally, cytokines are part of the body's immune response to infection, but sudden release in large quantities can cause multiple system organ failure and death. Cytokine storms can be caused by a number of infectious and non-infectious, particularly viral respiratory infections such as H1N1 influenza, H5N1 influenza, SARS-CoV-1, and SARS-CoV-2, influenza B, parainfluenza virus, and Ebola. . In particular, when inflammation occurs in the glomeruli and blood vessels, various symptoms such as cerebral infarction, spinal cord injury, paresthesia, and numbness in the hands and feet may appear. In addition, various symptoms such as chronic shortness of breath, pulsating headache, glomerulonephritis, chronic inflammatory sinusitis, and visual disturbances, such as throbbing blood vessels in the head like a pulse, are also diseases that can appear when symptoms of vasculitis persist. The main symptoms of these vasculitis appear in various ways depending on the affected blood vessels, and cannot be prevented in advance due to the nature of autoimmune disease. The present invention promotes vascular inflammation and cancer proliferation and cell death by recombinant 8 natural herbal medicines (branch, yellow white, moth bark, mold, anti-inflammatory, goodwill, gumac, plantain taxa). In the case of normal cells, if DNA is damaged by any cause, the proliferation of abnormal cells is prevented. However, dead cells undergo abnormal growth due to genetic modification. Therefore, if dead cell changes due to pathogen drug appear with Corona-19 virus vaccine, apoptosis can be inferred, so 8 extracts of recombinant herbal medicines of natural substances prevent inflammation and cancer cells.

A cytokine storm, called cytokine hypersecretion (hypercytokinemia), is a physiological response in humans and other animals in which the innate immune system causes an uncontrolled and excessive release of inflammatory signaling molecules called cytokines. Normally, cytokines are part of the body's immune response to infection, but sudden release in large quantities can cause multiple system organ failure and death. Cytokine storms can be caused by a number of infectious and non-infectious, particularly viral respiratory infections such as H1N1 influenza, H5N1 influenza, SARS-CoV-1, and SARS-CoV-2, influenza B, parainfluenza virus, and Ebola. . Other causative agents include Epstein-Barr virus, cytomegalovirus, group A streptococci, and non-infectious conditions such as graft-versus-host disease. Viruses can invade lung epithelial cells and alveolar macrophages to produce viral nucleic acids, which stimulate infected cells to release cytokines and chemokines, and activate macrophages, dendritic cells, etc. Cytokine storm syndrome is a diverse set of conditions that can result in a cytokine storm. Cytokine storm syndrome includes familial serum cell lymphadenopathy, Epstein-Barr virus-associated serum cell lymphosis, systemic or non-systemic juvenile idiopathic arthritis-related bulimia activation syndrome, NLRC4 macrophagia activation syndrome, CYTOkine release syndrome and cytosis syndrome. .
Monocytosis Terminology
1. Chronic systemic inflammation (SI) results from the release of pro-inflammatory cytokines from immune-related cells and the chronic activation of the innate immune system. It contributes to the development or progression of certain conditions such as cardiovascular disease, cancer, diabetes mellitus, chronic kidney disease, non-alcoholic fatty liver disease, autoimmune and neurodegenerative diseases, and coronary heart disease.
2. Diabetes Heuer, FF; Chang, X; Coffin, E; Basamseti, SB; Modu, G; Schloss, MJ; Rhodes, D; McAlpine, CS; Iwamoto, Y; Libby, P; Naxerova, K; Swirski, F; Dutta, P; Nahrendorf, P (April 2020).” Bone marrow endothelial cells regulate bone marrow inclusion in diabetes.
3. Stress Response Heidt, Timo; Sager, Hendrick B.; Courtesy, Gabriel; duta, parta; Iwamoto, Yoshiko; Zaltsmann, Alex; von Zurmuelen, Constantine; Bode, Christophe; Frizione, Gregory L; Denninger, John; Lynn, Charles P; Vingoni, Claudio; Libby, Peter; Swirski, Philip K; Weissleder, Ralph; Narendorf, Mathias (July 2014). “Chronic variable stress activates hematopoietic stem cells.
: Physiological, biological or psychological stress is an organism's response to stress, such as environmental conditions. Stress is the body's way of responding to conditions such as threats, challenges, or physical and psychological barriers. Stimuli that change an organism's environment are responded to by multiple systems in the body. with humans
4. Cushing's syndrome (anaphylaxis)
5. Immune Mediated Diseases
6. Granulomatous Disease Granulomas are collections of macrophages that form in response to chronic inflammation. This occurs when the immune system attempts to sequester foreign substances that it cannot otherwise remove. These materials include infectious organisms, including bacteria and fungi, as well as other materials such as debris, dead skin cells, and suture fragments.
7. Atherosclerosis
8. Necrosis Necrosis is a form of cell damage that results in premature death of cells in living tissue by autolysis. Necrosis results from factors external to cells or tissues, such as infection, or trauma that results in extraneous digestion of cellular components. In contrast, apoptosis is a naturally occurring programmed form of cell death.
9. Erythrocytes Red blood cells (RBCs), also known as regenerating red blood cells (RBCs), are red blood cells, red blood cells (humans or other animals whose red blood cells do not have a nucleus), blood matids, erythrocytes or red blood cells (Greek red blood cells for "hollow blood vessels" in Greek and From chitos-cyte (translated as "cell" in modern usage), it is the most common type of blood cell and the primary means in vertebrates for delivering oxygen to body tissues
10, Sarcoidosis, Sarcoidosis is a disease involving an abnormal collection of inflammatory cells that form clumps known as granules. The disease usually begins in the lungs, skin, or lymph nodes. Less commonly affected are the eyes, liver, heart, and brain. However, any organ can be affected. Signs and symptoms are related
11, Chronic myeloid cell leukemia (CMML): Chronic myeloid cell leukemia (CMML) is a type of leukemia that is a cancer of the blood-forming cells in the bone marrow. In adults, blood cells are formed in the bone marrow, by a process known as hematopoiesis. In CMML, abnormalities in at least one type of blood cell, as well as increased numbers (blasts) of monocytes and immature blood cells in peripheral blood and bone marrow

Claims (11)

"Chronic, acute glomerulonephritis" treatment drug made up of 8 kinds of natural herbal medicines 8 types of recombinant herbal medicines of natural substances (Zhibuja, Yellowbaek, Mortice, Hyeonggae, Anti-inflammation, Good will, Gumaek, Plantain) and prepared membranous proliferative "glomerulonephritis" treatment drug Secondary “glomerulonephritis” treatment drug made up of 8 recombinant herbal medicines of natural substances (Zhibuja, Yellowbaek, Mortice, Hyeonggae, Anti-inflammation, Goodwill, Gumaek, Plantain) 8 types of natural herbal medicines recombinant (Zhibuja, yellow baekbaek, mothbaekpi, hyeonggae, anti-inflammation, goodwill, gumac, plantain taxa) and prepared "hyeolno, white medicine" therapeutic drug "Asymptomatic urinary abnormality" treatment drug composed and prepared of 8 kinds of natural herbal medicines "IgA nephropathy" treatment drug composed and prepared of 8 kinds of natural herbal medicines 8 recombinant herbal medicines made of natural substances (Globular sclerosis) treatment drug made up of 8 kinds of recombinant (Zhibuja, Yellowbaek, Mortice bark, Hyeonggae, Anti-inflammation, Goodwill, Gumaek, Plantain taxa) 8 kinds of recombinant herbal medicines of natural substances (Zhibuja, yellow white, moth bark, mold, anti-inflammation, goodwill, gumac, plantain taxa) and prepared "nephrotic syndrome" treatment drug "Rapid progressive glomerulitis" treatment drug made up of 8 kinds of recombinant herbal medicines of natural substances (Zhibuja, yellow white, moth bark, mold, anti-inflammatory, bona fide, gumac, plantain taxa) Made up of 8 recombinant herbal medicines of natural substances (Zhibuja, yellow baekbaek, moth bark, hyeonggae, anti-inflammatory, bona fide, gumac, and plantain taxa) and "membranous nephropathy" treatment drug Made up of 8 recombinant herbal medicines of natural substances (Zhibuja, yellow white, moth bark, mold, anti-inflammatory, bona fide, gumac, plantain) and treats "micro lesions"