KR20230102231A - Cell culture control system and method thereof - Google Patents

Abstract

The present invention relates to a cell culture control system and method, and the cell culture control system according to the present invention collects measurement data on cell culture from a plurality of cell culture devices located in a remote location, and the collected measurement data and pre-stored references. and a cell culture control device that generates management data related to cell culture based on a result of comparing and analyzing the data and provides the data to a plurality of cell culture devices. According to the present invention, it is possible to more efficiently control and manage a plurality of cell cultivators located in remote locations to satisfy the needs of various users having different cell culture conditions.

Description

Cell culture control system and method {CELL CULTURE CONTROL SYSTEM AND METHOD THEREOF}

The present invention relates to a cell culture control system and method, and more particularly, to a cell culture control system and method capable of controlling and managing a plurality of cell culture devices located in remote locations.

In general, cell culture is to remove tissue pieces aseptically from a multicellular organism, provide nutrients, and culture and proliferate in a container. For cell culture, a culture chamber providing a constant space in which cells can be cultured, nutrients to cells A culture medium or various gases to supply is required. In particular, after being injected into the culture chamber and used for cell culture, the culture medium or various gases is exchanged at an appropriate cycle to keep the cell tissue in a fresh state.

Among the various fields using cell culture, the field of cell therapy is in the beginning stage, and the fields of pharmaceutical production, animal/plant breeding, and alternative meat or feed production are in a highly developed stage.

Culture used in the field of cell therapy or culture for producing a small amount of substitute meat or synthetic meat requires a small-scale culture facility, but it is difficult to build a small-scale manpower and system to control and manage it.

Accordingly, there was a problem in that it could not satisfy the various needs of consumers who wanted cell culture. In particular, there was a problem of having to bear relatively high costs for small-scale culture required for research culture.

In order to solve the above problems, a system for dramatically reducing culture costs and satisfying the various needs of consumers by allowing a plurality of consumers to share and manage cell culture devices, equipment, various technologies, knowledge, software, etc. this is being requested

Korean Patent Publication No. 2014-0014494 (Publication date: 2014. 02. 06)

An object of the present invention is to provide a cell culture control system and method capable of satisfying the needs of various users having different cell culture conditions by more efficiently controlling and managing a plurality of cell culture devices located in remote locations.

The tasks of the present invention are not limited to the tasks mentioned above, and other tasks not mentioned will be clearly understood by those skilled in the art from the following description.

A cell culture control system according to an embodiment of the present invention for solving the above technical problem collects measurement data about cell culture from a plurality of cell culture devices located in a remote location, and collects the collected measurement data and pre-stored reference data. and a cell culture control device that generates management data related to cell culture based on the results of the analysis by comparing and providing the data to the plurality of cell cultivators.

The cell culture control device receives a cell culture requirement from at least one terminal device, compares the received cell culture requirement and the measurement data with the reference data, and generates the management data using an analysis result. , The generated management data may be provided to the plurality of cell cultivators and the terminal device, respectively.

The cell culture control device may calculate payment information based on the received cell culture requirements and the generated management data.

The calculated payment information may include information on periodic payment service usage and payment amount.

The cell culture control device may collect the measurement data including first measurement data about cells, second measurement data about culture conditions, and third measurement data about culture operation.

The cell culture control device may compare the collected measurement data with the reference data and receive an analysis result from an external analysis server.

The management data may include operation data for operating the plurality of cell cultivators.

On the other hand, the cell culture control method according to an embodiment of the present invention includes the steps of collecting measurement data about cell culture from a plurality of cell culture devices located in a remote location by a cell culture control device, the collected measurement data and pre-stored reference data. It may include generating management data related to cell culture based on a result of comparing and analyzing, and providing the generated management data to the plurality of cell cultivators.

and receiving, by the cell culture control device, cell culture requirements from at least one terminal device, and generating the management data includes the received cell culture requirements, the measurement data, and the reference data. The step of generating the management data using the results of the comparison and analysis and providing the generated management data to the plurality of cell cultivators includes the generated management data to the plurality of cell cultivators and the terminal device, respectively. can provide

Calculating payment information based on the received cell culture requirements and the generated management data may be further included.

The calculated payment information may include information on periodic payment service usage and payment amount.

The measurement data may include first measurement data about cells, second measurement data about culture conditions, and third measurement data about culture operation.

A result of analysis by comparing the collected measurement data with the reference data may be received from an external analysis server.

The management data may include operation data for operating the plurality of cell cultivators.

As described above, according to the cell culture control system and method according to an embodiment of the present invention, it is possible to more efficiently control and manage a plurality of cell culture devices located in remote locations to satisfy the needs of various users having different cell culture conditions. there is

That is, by contracting a plurality of cell cultivators with different users and paying the payment through a regular payment method, the various cell culture needs of users are met, the management cost for shared manpower and equipment is lowered, and each user's needs are met. It has the advantage of being able to operate different culture types and scales.

In this way, a plurality of users share and manage various technologies, knowledge, software, etc., such as devices or facilities for cell culture, joint purchase of culture media, supply or replacement of consumables, measures after determining the culture status, and active maintenance transport. By doing so, it has the advantage of being able to drastically reduce the culture cost and satisfy the various needs of a plurality of users.

Moreover, as the level of the user's culture skill and knowledge increases, the overall cost decreases because the user who has to pay the cost reduces as much as the user willing to take responsibility for the culture result, which may vary depending on the cell. It has the advantage of giving social motivation to acquire culture skills.

Effects according to the present invention are not limited by the contents exemplified above, and more various effects are included in the present specification.

1 is a configuration diagram schematically showing a cell culture control system according to an embodiment of the present invention.
Fig. 2 is a block diagram of the cell culture control device shown in Fig. 1;
3 is an operational flow chart showing a cell culture control process according to an embodiment of the present invention.

Advantages and features of the present invention, and methods for achieving them will become clear with reference to the detailed embodiments described later in conjunction with the accompanying drawings.

However, the present invention is not limited to the embodiments disclosed below and will be implemented in various different forms.

This embodiment in this specification is provided to complete the disclosure of the present invention, and to completely inform those skilled in the art of the scope of the invention to which the present invention belongs.

And the invention is only defined by the scope of the claims. Thus, in some embodiments, well-known components, well-known operations and well-known techniques have not been described in detail in order to avoid obscuring the interpretation of the present invention.

In addition, like reference numerals designate like components throughout the specification, and terms used (referred to) in this specification are for describing embodiments and are not intended to limit the present invention.

In this specification, the singular also includes the plural unless specifically stated in the phrase, and components and operations referred to as 'comprising (or including)' do not exclude the presence or addition of one or more other components and operations. .

Unless otherwise defined, all terms (including technical and scientific terms) used in this specification may be used in a meaning commonly understood by those of ordinary skill in the art to which the present invention belongs.

In addition, terms defined in commonly used dictionaries are not interpreted ideally or excessively unless they are defined.

Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings.

1 is a configuration diagram schematically showing a cell culture control system according to an embodiment of the present invention, and FIG. 2 is a block diagram of the cell culture control device shown in FIG. 1 .

As shown in FIGS. 1 and 2 , the cell culture control system 1 includes a plurality of cell culture devices 100 , a cell culture control device 200 and at least one terminal device 300 .

The plurality of cell cultivators 100 are located in a remote location and may include a culture chamber having a predetermined space therein for culturing cells. The inside of the cell cultivator 100 is in a state optimized for culturing cells, and maintains an environment suitable for cell culture or differentiation and cell growth, and a device for supplying a culture medium to cells, a device for supplying various gases, etc. Alternatively, a device capable of suppressing contamination may be installed.

The cell culture control device 200 is a device for remotely controlling the plurality of cell cultivators 100, and includes a collection unit 210, a storage unit 230, a control unit 250, and a communication unit 270. .

The collection unit 210 may collect measurement data about cell culture from the plurality of cell cultivators 100 .

More specifically, the collection unit 210 may collect measurement data including first measurement data on cells, second measurement data on culture conditions, and third measurement data on culture operation. Here, the first measurement data is a cell image representing a cell culture state, and may include parameters such as cell number, cell size, cell shape, cell movement distance, number of cell divisions, or cell brightness.

For this purpose, the collecting unit 210 may include an image obtaining means for obtaining a video image of the cell and an image editing means for editing the video image of the cell in a desired form. The image acquisition means is generally a means for observing a cell image, and a phase contrast microscope using direct illumination is applied, a CCD (Charge Coupled Device) camera using indirect illumination and a reflector, a CMOS (Complementary Metal Oxide Semiconductor) sensor, or A microscope for cell observation or the like can be applied. A cell imaging image can be easily acquired using such a microscope, and at this time, the cell imaging image may be in the form of a photograph or a video. The image editing unit may enlarge, reduce, move, rotate, or change lighting or background settings of the obtained cell image image so that the user can easily observe the obtained cell image image.

The collection unit 210 may collect second measurement data related to culture conditions such as temperature, acidity (pH), osmotic pressure, pressure, or turbidity, which are environmental information inside the cell incubator 100, such as electrical conductivity or centrifugal force. Similarly, the third measurement data for the operation of the device for operating the cell cultivator 100 may be continuously collected.

The storage unit 230 may store reference data related to cell culture. Here, the storage unit 230 may receive and store reference data related to cell culture from an external reference database, collect data provided from an external reference database, such as a cell culture specialized institution, and use the criteria for managing cell culture. can be used as data.

The control unit 250 is a component that controls the overall operation of the cell culture control device 200, and based on the result of analysis by comparing the measurement data collected in the collection unit 210 with the reference data stored in the storage unit 230 Administrative data on cell culture can be generated.

For example, the control unit 250 may compare and analyze cell image images collected at regular intervals with reference image images stored in the storage unit 230 to determine whether the cells to be measured are normally differentiated. After recognizing the video image through an image processing program, it is possible to determine normal differentiation of the target cell by comparing it with a pre-stored reference video image according to the time elapsed after the start of differentiation or measurement.

If it is determined that the cell to be measured is normally differentiated, the control unit 250 stores the operation information of the cell cultivator 100, the video image of the cell to be measured, and the differentiation result in the storage unit 230, and the resulting information is stored in the cell 230. It can be controlled to be transmitted to the incubator 100 and/or the terminal device 300 to be described below.

On the other hand, if it is determined that the target cell to be measured is not normally differentiated, the control unit 250 controls the video image in which the abnormality has occurred to be transmitted to the cell culture device 100 and/or the terminal device 300, and the terminal device 300 ), it is possible to control the output of a warning message or a warning sound to guide that an abnormality has occurred.

In addition, the control unit 250 may generate management data capable of taking measures against abnormalities generated in the cell culture device 100 or during the cell culture process, and control the data to be transmitted to the cell culture device 100 . Here, the management data may include operational data for operating the cell culture machine. For example, the control unit 250 may generate operation data for adjusting the intensity of light irradiated for cell culture, irradiation time, etc., and control the data to be transmitted to the cell culture device 100, and detailed information about the operation of the cell culture device is It can be controlled to be transmitted to the cell culture device 100 and/or the terminal device 300.

As such, the management data may include information necessary for managing the cell culture device, such as operation, maintenance, repair, disinfection, cleaning, and replacement of consumables, and may include information necessary for manager's operation, such as maintenance of the cell culture device or replacement of consumables. In the case of management, the corresponding management data may be transmitted to the manager's terminal device, and the manager may directly perform the control.

Meanwhile, the control unit 250 may receive an analysis result by comparing the collected measurement data with reference data from an external analysis server, and may generate management data based on the received analysis result. At this time, the external analysis server may be composed of a server operated by a cell culture expert or a server operated by a professional analysis program or artificial intelligence algorithm.

In this way, it is possible to more efficiently manage cell culture by configuring a network between a plurality of cell cultivators and the cell culture control device to monitor and manage the cell culture process in real time.

The control unit 250 may receive cell culture requirements from the terminal device 300, compare the received cell culture requirements and measurement data with reference data, and generate management data using an analysis result. Here, the cell culture requirements may include environmental requirements such as temperature/humidity/carbon dioxide in the cell culture device 100 and operating requirements such as irradiated light intensity and irradiation time.

For example, the control unit 250 receives cell culture requirements from the terminal device 300, and management data capable of controlling the environment inside the cell culture device 100 based on the received cell culture requirements and analysis results. may be generated and provided to the cell culture device 100 and/or the terminal device 300. That is, the control unit 250 generates management data including at least one of different magnetic field strengths, frequencies, times, and patterns in order to control the light irradiation device or the magnetic field irradiation device provided inside the cell culture device 100. and provided to the cell culture device 100 and/or the terminal device 300.

At this time, when there are a plurality of terminal devices, since cell culture requirements are received for each of the plurality of terminal devices and different management data can be generated in response to the received cell culture requirements, users having different cell culture conditions It has the advantage of being able to satisfy various needs.

The controller 250 may calculate payment information based on the received cell culture requirements and the generated management data. Here, the calculated payment information may include information on periodic payment service use and payment amount. That is, the regular payment service is to rent and use the cell culture device 100 on a regular basis, and the controller 250 can determine whether to use the regular payment service according to the cell culture requirements, The payment amount can be calculated differently depending on the level of management difficulty. For example, when using a regular payment service, the payment amount is calculated by considering the number of users using the regular payment service, and when using the analysis result received from an external analysis server, it is classified as a separate amount to provide payment information. can be calculated, and when direct manipulation by a manager is required in the management of cell culture, payment information can be calculated by separately setting the manager's cost.

In this way, a plurality of users having different cell culture requirements can reduce the burden of management cost by using a regular payment method to pay the payment amount due to sharing and using a plurality of cell cultivators 100 located in remote locations. make it possible At this time, the amount of payment varies depending on the type and difficulty of entrusted management, and self-cost may be paid for each entrusted part.

In addition, by using the user's cell culture requirements, etc., cell culture-related management data is created and provided, and when a subscription to cell culture is received, management of the corresponding cell culture is performed during the subscription period, thereby providing user convenience and There are advantages to increasing satisfaction.

In this way, through the cell culture sharing subscription service, a plurality of users can use the plurality of cell cultivators 100 at low cost without any burden, and since the amount is paid in a regular payment method, the plurality of cells can be more conveniently and efficiently without burden. Incubators 100 may be used.

In addition, the indirect management function allows users to share situational awareness and knowledge about cell culture with other users.

The communication unit 270 may exchange various types of information with the plurality of cell cultivators 100 and the plurality of terminal devices 300 through the communication network 10 .

Here, the communication network 10 includes a Local Area Network (LAN), a Metropolitan Area Network (MAN), a Wide Area Network (WAN), the Internet, and a 3G/4G/5G (generation) ) may include various data communication networks including mobile communication networks, Wi-Fi, WIBRO (Wireless Broadband Internet) or LTE (Long Term Evolution), etc., regardless of wired or wireless communication method does not exist.

The communication unit 270 may transmit the generated management data to the plurality of cell cultivators 100 and/or the plurality of terminal devices 300 .

The terminal device 100 is composed of a plurality of pieces, and includes a personal computer (PC), a smart phone, a tablet PC, a personal digital assistant (PDA) or a web pad. ), etc., and may be a terminal equipped with a microprocessor and equipped with an arithmetic capability.

The terminal device 300 is a terminal used by a user requesting cell culture or a manager who performs management by the user requesting cell culture, and an application or program providing a cell culture service may be installed.

The terminal device 300 transmits cell culture requirements to the cell culture control device 200 and receives management data transmitted from the cell culture control device 200 to determine the cell culture state or manage the cell culture device 100. status, etc.

In this case, the management data may include data for manipulating and managing devices in the cell culture device 100, such as device maintenance, repair, disinfection, cleaning, and replacement of consumables, or action data related to cell culture.

In this way, a plurality of users share and manage various technologies, knowledge, software, etc., such as devices or facilities for cell culture, joint purchase of culture media, supply or replacement of consumables, measures after determining the culture status, and active maintenance transport. By doing so, it has the advantage of being able to drastically reduce the culture cost and satisfy the various needs of a plurality of users.

Hereinafter, a cell culture control method according to an embodiment of the present invention will be described.

Figure 3 shows an operational flow chart showing a cell culture control process according to an embodiment of the present invention.

Referring to FIG. 3 , the cell culture control device 200 may collect measurement data about cell culture from a plurality of cell culture devices located in a remote location (S300).

More specifically, the cell culture control device 200 may collect measurement data including first measurement data on cells, second measurement data on culture conditions, and third measurement data on culture operation. Here, the first measurement data is a cell image representing a cell culture state, and may include parameters such as cell number, cell size, cell shape, cell movement distance, number of cell divisions, or cell brightness.

In addition, the cell culture control device 200 may collect second measurement data related to culture conditions such as temperature, acidity (pH), osmotic pressure, pressure, or turbidity, which is environmental information inside the cell culture device 100, and Third measurement data about the operation of the device for operating the cell culture device 100, such as conductivity or centrifugal force, may be continuously collected.

Next, the cell culture control device 200 compares the collected measurement data with the pre-stored reference data to generate cell culture-related management data based on the analysis result (S310), and transfers the generated management data to the cell culture device (S310). 100) and/or the terminal device 300.

For example, the cell culture control device 200 may compare and analyze cell image images collected at regular intervals with reference image images stored in advance to determine whether cells to be measured are normally differentiated. After recognizing the image through an image processing program, normal differentiation of the cells to be measured may be determined by comparing the image with a pre-stored reference image according to the time elapsed after the start of differentiation or measurement.

If it is determined that the cell to be measured is normally differentiated, the cell culture control device 200 stores operation information of the cell culture device 100, a video image of the cell to be measured, and a differentiation result, and the resulting information is stored in the cell culture device 100. ) and/or may be transmitted to the terminal device 300.

On the other hand, the cell culture control device 200 transmits a video image in which an abnormality occurs to the cell culture device 100 and/or the terminal device 300 when it is determined that the target cell to be measured is not normally differentiated, and the terminal device ( 300), it is possible to control the output of a warning message or a warning sound informing that an abnormality has occurred.

In addition, the cell culture control device 200 may generate and transmit management data to the cell culture device 100 or the cell culture device 100 to deal with abnormalities generated during the cell culture process. Here, the management data may include operational data for operating the cell culture machine. For example, the cell culture control device 200 may generate and transmit operating data for adjusting the intensity of light irradiated for cell culture, irradiation time, etc. to the cell culture device 100, and detailed information about the operation of the cell culture device is It can be transmitted to the cell culture device 100 and/or the terminal device 300.

As such, the management data may include information necessary for managing the cell culture device, such as operation, maintenance, repair, disinfection, cleaning, and replacement of consumables, and may include information necessary for manager's operation, such as maintenance of the cell culture device or replacement of consumables. In the case of management, the corresponding management data may be transmitted to the manager's terminal device, and the manager may directly perform the control.

On the other hand, the cell culture control device 200 compares the collected measurement data with the reference data, can receive an analysis result from an external analysis server, and can generate management data based on the received analysis result. At this time, the external analysis server may be composed of a server operated by a cell culture expert or a server operated by a professional analysis program or artificial intelligence algorithm.

In this way, it is possible to more efficiently manage cell culture by configuring a network between a plurality of cell cultivators and the cell culture control device to monitor and manage the cell culture process in real time.

The cell culture control device 200 receives the cell culture requirements from the terminal device 300, compares the received cell culture requirements and measurement data with reference data, and generates management data using an analysis result. . Here, the cell culture requirements may include environmental requirements such as temperature/humidity/carbon dioxide in the cell culture device 100 and operating requirements such as irradiated light intensity and irradiation time.

For example, the cell culture control device 200 may receive cell culture requirements from the terminal device 300 and control the environment inside the cell culture device 100 based on the received cell culture requirements and analysis results. Managed data may be generated and provided to the cell culture device 100 and/or the terminal device 300 . That is, the cell culture control device 200 controls the light irradiation device or the magnetic field irradiation device provided inside the cell culture device 100, and manages at least one of different magnetic field strengths, frequencies, times, and patterns. Data may be generated and provided to the cell culture device 100 and/or the terminal device 300 .

At this time, when there are a plurality of terminal devices, since cell culture requirements are received for each of the plurality of terminal devices and different management data can be generated in response to the received cell culture requirements, users having different cell culture conditions It has the advantage of being able to satisfy various needs.

The cell culture control device 200 may calculate payment information based on the received cell culture requirements and the generated management data. Here, the calculated payment information may include information on periodic payment service use and payment amount. That is, the regular payment service is to rent and use the cell culture device 100 on a regular basis, and the cell culture control device 200 can determine whether to use the regular payment service according to the cell culture requirements, and use the regular payment service and The payment amount may be calculated differently depending on the difficulty of managing cell culture. For example, when using a regular payment service, the payment amount is calculated by considering the number of users using the regular payment service, and when using the analysis result received from an external analysis server, it is classified as a separate amount to provide payment information. can be calculated, and when direct manipulation by a manager is required in the management of cell culture, payment information can be calculated by separately setting the manager's cost.

In this way, a plurality of users share and manage various technologies, knowledge, software, etc., such as devices or facilities for cell culture, joint purchase of culture media, supply or replacement of consumables, measures after determining the culture status, and active maintenance transport. By doing so, it has the advantage of being able to drastically reduce the culture cost and satisfy the various needs of a plurality of users.

Those skilled in the art to which the present invention pertains will understand that the present invention can be embodied in other specific forms without changing its technical spirit or essential features. Therefore, the embodiments described above should be understood as illustrative in all respects and not limiting. The scope of the present invention is indicated by the following claims rather than the detailed description above, and all changes or modifications derived from the meaning and scope of the claims and equivalent concepts should be construed as being included in the scope of the present invention. do.

The present invention can be used for cell culture control systems and methods.

1: cell culture control system 100: cell incubator
200: cell culture control device 210: collection unit
230: storage unit 250: control unit
270: communication unit 300: terminal device

Claims (14)

Cell culture measurement data is collected from a plurality of cell cultivators located in remote locations, and cell culture management data is generated based on the analysis result by comparing the collected measurement data with pre-stored reference data. Including a cell culture control device provided to the cell cultivators of,
Cell culture control system. According to claim 1,
The cell culture control device,
Receive cell culture requirements from at least one terminal device;
Generating the management data using a result of analysis by comparing the received cell culture requirements and the measurement data with the reference data;
Providing the generated management data to the plurality of cell cultivators and the terminal device, respectively.
Cell culture control system. According to claim 2,
The cell culture control device,
Calculating payment information based on the received cell culture requirements and the generated management data,
Cell culture control system. According to claim 3,
The calculated payment information,
Including information about the use of subscription services and payment amounts;
Cell culture control system. According to claim 1,
The cell culture control device,
Collecting the measurement data including first measurement data about the cells, second measurement data about the culture conditions, and third measurement data about the culture operation,
Cell culture control system. According to claim 1,
The cell culture control device,
Comparing the collected measurement data with the reference data and receiving an analysis result from an external analysis server,
Cell culture control system. According to claim 1,
The management data,
Including operating data for operating the plurality of cell cultivators,
Cell culture control system. Collecting measurement data related to cell culture from a plurality of cell culture devices located in a remote location by a cell culture control device;
Generating management data related to cell culture based on a result of analysis by comparing the collected measurement data with pre-stored reference data, and
Comprising the step of providing the generated management data to the plurality of cell cultivators,
Cell culture control methods. According to claim 8,
The cell culture control device further comprising receiving a cell culture requirement from at least one terminal device,
The step of generating the management data,
Generating the management data using a result of analysis by comparing the received cell culture requirements and the measurement data with the reference data;
Providing the generated management data to the plurality of cell cultivators,
Providing the generated management data to the plurality of cell cultivators and the terminal device, respectively.
Cell culture control methods. According to claim 9,
Further comprising calculating payment information based on the received cell culture requirements and the generated management data.
Cell culture control methods. According to claim 10,
The calculated payment information,
Including information about the use of subscription services and payment amounts;
Cell culture control methods. According to claim 8,
The measurement data,
Including first measurement data about cells, second measurement data about culture conditions, and third measurement data about culture operations,
Cell culture control methods. According to claim 8,
The result of analysis by comparing the collected measurement data with the reference data,
Received from an external analysis server,
Cell culture control methods. According to claim 8,
The management data,
Including operating data for operating the plurality of cell cultivators,
Cell culture control methods.

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