KR20230092183A - Kompetitive allele specific PCR primer set for distinguishing sesame cultivars, constructing genetic map of sesame and use thereof - Google Patents

Abstract

The present invention relates to a KASP (kompetitive allele specific PCR) primer set and its use for determining domestic varieties of sesame seeds and creating a genetic linkage map. It can promote gene discovery and can be usefully used in sesame molecular breeding research.

Description

KASP primer set for sesame cultivar identification and genetic association map creation and its use {Kompetitive allele specific PCR primer set for distinguishing sesame cultivars, constructing genetic map of sesame and use thereof}

The present invention relates to a KASP primer set for identifying sesame varieties and creating a genetic linkage map.

Sesame is one of the representative oilseed crops in the world including Korea. It is mainly consumed as sesame oil, and is also widely used as an ingredient for sesame salt and desserts such as sesame sesame seeds. Recently, various functional ingredients such as oleic acid and linoleic acid, which are high-quality fatty acids contained in sesame seeds, sesamin-like substances, which are antioxidants, and lecithin, which are good for the brain, have been reported and are widely illuminated as a health functional food. In Korea, the cultivation area is gradually decreasing due to climate change and difficulties in mechanization, and imported sesame is replacing it.

Nevertheless, consumers and industries have a high demand for domestic varieties such as varieties with high content of functional substances, varieties resistant to diseases and pests in response to climate change, and varieties suitable for mechanization, so it is necessary to develop high-quality excellent varieties continuously.

In order to develop varieties desired by farmers, industries, and consumers, molecular breeding methods such as marker development and genetic isolation through useful trait gene mapping are required, but sesame is a very weak domestic molecular breeding base compared to other crops such as rice and soybeans. On the other hand, markers for genetic mapping have not yet been reported in large quantities.

Sesame is a self-fertilized crop, but when it is actually grown in the field, 2 to 5% of natural hybridization can lead to mixing of genotypes between varieties. It is necessary to develop a discrimination technology that can determine whether the breed is mixed and authentic by profiling the genotype of each domestic breed.

Previously reported DNA markers for SSR and TAIL-PCR are not suitable for large-scale analysis because the identification method is not simple, takes a long time, and requires a lot of labor and cost. In particular, a small amount of markers between 6 and 27 for each type of DNA marker was reported, which is a very insufficient amount to create a genetic linkage map of 13 chromosomes of sesame seeds.

Under this background, the present inventors developed molecular markers that are evenly distributed throughout the sesame genome to support molecular breeding for the development of domestic sesame varieties, and on the other hand, selected marker sets that can determine the authenticity and incorporation of domestic varieties. . KASP markers, which are cheap for marker analysis, have high reproducibility, and can be analyzed in large quantities in a short period of time, have been developed in large quantities.

One object of the present invention is a primer for KASP marker amplification for sesame gene association map creation comprising 19 or more primer sets selected from the group consisting of 331 primer sets consisting of oligonucleotides of SEQ ID NO: 1 to SEQ ID NO: 993 to provide a set.

Another object of the present invention is to provide a KASP marker kit for creating a sesame gene association map, including the primer set.

Another object of the present invention is to provide a primer set for discriminating sesame variety comprising 19 or more primer sets selected from the group consisting of 331 primer sets consisting of oligonucleotides of SEQ ID NO: 1 to SEQ ID NO: 993. .

Another object of the present invention is to provide a kit for identifying sesame varieties comprising the above composition.

Another object of the present invention is a) separating genomic DNA from sesame seeds; (b) performing KASP using the primer set using the genomic DNA of step (a) as a template; and (c) analyzing the amplification product of the KASP, to provide a method for determining a sesame variety using genomic DNA isolated from a sesame sample and the primer set.

According to one embodiment of the present invention, in one embodiment, the present invention is a sesame variety comprising 19 or more primer sets selected from the group consisting of 331 primer sets consisting of oligonucleotides of SEQ ID NO: 1 to SEQ ID NO: 993 A set of primers for amplifying KASP markers for genetic mapping is provided.

The KASP marker set for genetic mapping includes a first allele-specific forward primer represented by SEQ ID NO: 1 for SK01_007, a second allele-specific forward primer represented by SEQ ID NO: 2, and a reverse primer set represented by SEQ ID NO: 3, The first allele-specific forward primer represented by SEQ ID NO: 4 for SK01_012, the second allele-specific forward primer represented by SEQ ID NO: 5, and the reverse primer set represented by SEQ ID NO: 6, SEQ ID NO: 7 for SK01_018 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 8 and the reverse primer set represented by SEQ ID NO: 9, the first allele-specific forward primer represented by SEQ ID NO: 10 for SK01_025, sequence The second allele-specific forward primer represented by No. 11 and the reverse primer set represented by SEQ ID NO: 12, the first allele-specific forward primer represented by SEQ ID NO: 13 for SK01_030, the second allele represented by SEQ ID NO: 14 A set of specific forward primers and reverse primers represented by SEQ ID NO: 15, a first allele-specific forward primer represented by SEQ ID NO: 16 for SK01_038, a second allele-specific forward primer represented by SEQ ID NO: 17, and SEQ ID NO: 18 a reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 19 for SK01_046, a second allele-specific forward primer represented by SEQ ID NO: 20, and a reverse primer set represented by SEQ ID NO: 21, sequence for SK01_054 The first allele-specific forward primer represented by SEQ ID NO: 22, the second allele-specific forward primer represented by SEQ ID NO: 23, and the reverse primer set represented by SEQ ID NO: 24, the first allele represented by SEQ ID NO: 25 for SKJ01_070 A specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 26 and a set of reverse primers represented by SEQ ID NO: 27, a first allele-specific forward primer represented by SEQ ID NO: 28 for SK01_084, represented by SEQ ID NO: 29 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 30, a first allele-specific forward primer represented by SEQ ID NO: 31 for SK01_093, a second allele-specific forward primer represented by SEQ ID NO: 32, and A reverse primer set represented by SEQ ID NO: 33, a first allele-specific forward primer represented by SEQ ID NO: 34 for SK01_102, a second allele-specific forward primer represented by SEQ ID NO: 35, and a reverse primer set represented by SEQ ID NO: 36 , the first allele-specific forward primer represented by SEQ ID NO: 37 for SK01_109, the second allele-specific forward primer represented by SEQ ID NO: 38 and the reverse primer set represented by SEQ ID NO: 39, SEQ ID NO: 40 for SK01_113 The first allele-specific forward primer represented by SEQ ID NO: 41, the second allele-specific forward primer represented by SEQ ID NO: 42, and the set of reverse primers represented by SEQ ID NO: 42, the first allele-specific forward primer represented by SEQ ID NO: 43 for SK01_120 , the second allele-specific forward primer represented by SEQ ID NO: 44 and the reverse primer set represented by SEQ ID NO: 45, the first allele-specific forward primer represented by SEQ ID NO: 46 for SK01_128, the second allele-specific forward primer represented by SEQ ID NO: 47 An allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 48, a first allele-specific forward primer represented by SEQ ID NO: 49 for SK01_133, a second allele-specific forward primer represented by SEQ ID NO: 50, and SEQ ID NO: 51 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 52 for SK01_139, a second allele-specific forward primer represented by SEQ ID NO: 53, and a reverse primer set represented by SEQ ID NO: 54, SK01_144 A set of a first allele-specific forward primer represented by SEQ ID NO: 55, a second allele-specific forward primer represented by SEQ ID NO: 56, and a reverse primer set represented by SEQ ID NO: 57, a first set represented by SEQ ID NO: 58 for SK01_152 An allele-specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 59 and a set of reverse primers represented by SEQ ID NO: 60, a first allele-specific forward primer represented by SEQ ID NO: 61 for SK01_160, SEQ ID NO: 62 A second allele-specific forward primer represented by and a reverse primer set represented by SEQ ID NO: 63, a first allele-specific forward primer represented by SEQ ID NO: 64 for SK01_174, and a second allele-specific forward primer represented by SEQ ID NO: 65 Primer and reverse primer set represented by SEQ ID NO: 66, first allele-specific forward primer represented by SEQ ID NO: 67 for SK01_181, second allele-specific forward primer represented by SEQ ID NO: 68, and reverse represented by SEQ ID NO: 69 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 70 for SK01_187, a second allele-specific forward primer represented by SEQ ID NO: 71, and a reverse primer set represented by SEQ ID NO: 72, SEQ ID NO: 73 for SK01_194 The first allele-specific forward primer represented by SEQ ID NO: 74, the second allele-specific forward primer represented by SEQ ID NO: 75, and the reverse primer set represented by SEQ ID NO: 75, the first allele-specific forward primer represented by SEQ ID NO: 76 for SK01_201 Primers, the second allele-specific forward primer represented by SEQ ID NO: 77 and the reverse primer set represented by SEQ ID NO: 78, the first allele-specific forward primer represented by SEQ ID NO: 79 for SK02_007, the first allele-specific forward primer represented by SEQ ID NO: 80 2 allele-specific forward primer and reverse primer set represented by SEQ ID NO: 81, the first allele-specific forward primer represented by SEQ ID NO: 82 for SK02_013, the second allele-specific forward primer represented by SEQ ID NO: 83 and SEQ ID NO: A reverse primer set represented by 84, a first allele-specific forward primer represented by SEQ ID NO: 85 for SK02_022, a second allele-specific forward primer represented by SEQ ID NO: 86, and a reverse primer set represented by SEQ ID NO: 87, A set of first allele-specific forward primers represented by SEQ ID NO: 88 for SK02_027, second allele-specific forward primers represented by SEQ ID NO: 89 and reverse primers represented by SEQ ID NO: 90, SEQ ID NO: 91 for SK02-036 The first allele-specific forward primer represented by SEQ ID NO: 92, the second allele-specific forward primer represented by SEQ ID NO: 93, and the reverse primer set represented by SEQ ID NO: 93, the first allele-specific represented by SEQ ID NO: 94 for SK02_041 Forward primer, second allele-specific forward primer represented by SEQ ID NO: 95 and reverse primer set represented by SEQ ID NO: 96, first allele-specific forward primer represented by SEQ ID NO: 97 for SK02_050, SEQ ID NO: 98 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 99, a first allele-specific forward primer represented by SEQ ID NO: 100 for SK02_059, and a second allele-specific forward primer represented by SEQ ID NO: 101 and a reverse primer set represented by SEQ ID NO: 102, a first allele-specific forward primer represented by SEQ ID NO: 103 for SK02_070, a second allele-specific forward primer represented by SEQ ID NO: 104, and a reverse sequence represented by SEQ ID NO: 105. The anti-primer set, the first allele-specific forward primer represented by SEQ ID NO: 106 for SK02_076, the second allele-specific forward primer represented by SEQ ID NO: 107, and the reverse primer set represented by SEQ ID NO: 108, sequence for SK02_081 The first allele-specific forward primer represented by SEQ ID NO: 109, the second allele-specific forward primer represented by SEQ ID NO: 110, and the reverse primer set represented by SEQ ID NO: 111, the first allele represented by SEQ ID NO: 112 for SK02_087 A trait-specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 113 and a set of reverse primers represented by SEQ ID NO: 114, a first allele-specific forward primer represented by SEQ ID NO: 115 for SK02_089, SEQ ID NO: 116 A second allele-specific forward primer represented by and a reverse primer set represented by SEQ ID NO: 117, a first allele-specific forward primer represented by SEQ ID NO: 118 for SK02_097, and a second allele-specific represented by SEQ ID NO: 119 A forward primer and a reverse primer set represented by SEQ ID NO: 120, a first allele-specific forward primer represented by SEQ ID NO: 121 for SK02_103, a second allele-specific forward primer represented by SEQ ID NO: 122, and SEQ ID NO: 123 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 124 for SK02_110, a second allele-specific forward primer represented by SEQ ID NO: 125, and a reverse primer set represented by SEQ ID NO: 126, SK02_117 The first allele-specific forward primer represented by SEQ ID NO: 127, the second allele-specific forward primer represented by SEQ ID NO: 128, and the reverse primer set represented by SEQ ID NO: 129, the first allele-specific forward primer represented by SEQ ID NO: 130 for SK02_121 1 allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 131 and the reverse primer set represented by SEQ ID NO: 132, the first allele-specific forward primer represented by SEQ ID NO: 133 for SK02_133, sequence The second allele-specific forward primer represented by No. 134 and the reverse primer set represented by SEQ ID NO: 135, the first allele-specific forward primer represented by SEQ ID NO: 136 for SK02_140, the second allele represented by SEQ ID NO: 137 A trait-specific forward primer and a reverse primer set represented by SEQ ID NO: 138, a first allele-specific forward primer represented by SEQ ID NO: 139 for SK02_151, a second allele-specific forward primer represented by SEQ ID NO: 140, and SEQ ID NO: 141 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 142 for SK02_160, a second allele-specific forward primer represented by SEQ ID NO: 143, and a reverse primer set represented by SEQ ID NO: 144, A set of a first allele-specific forward primer represented by SEQ ID NO: 145 for SK02_167, a second allele-specific forward primer represented by SEQ ID NO: 146 and a reverse primer set represented by SEQ ID NO: 147, SEQ ID NO: 148 for SK02_179 A first allele-specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 149, and a reverse primer set represented by SEQ ID NO: 150, the first allele-specific forward primer represented by SEQ ID NO: 151 for SK03_003 , the second allele-specific forward primer represented by SEQ ID NO: 152 and the reverse primer set represented by SEQ ID NO: 153, the first allele-specific forward primer represented by SEQ ID NO: 154 for SK03_012, the first allele-specific forward primer represented by SEQ ID NO: 155 2 allele-specific forward primers and reverse primer set represented by SEQ ID NO: 156, the first allele-specific forward primer represented by SEQ ID NO: 157 for SK03_020, the second allele-specific forward primer represented by SEQ ID NO: 158 and sequence Reverse primer set represented by No. 159, the first allele-specific forward primer represented by SEQ ID NO: 160 for SK03_029, the second allele-specific forward primer represented by SEQ ID NO: 161, and the reverse primer represented by SEQ ID NO: 162 Set, first allele specific forward primer represented by SEQ ID NO: 163 to SK03_030, second allele specific forward primer represented by SEQ ID NO: 164 and reverse primer set represented by SEQ ID NO: 165, SEQ ID NO: 166 to SK03_038 The first allele-specific forward primer represented by SEQ ID NO: 167, the second allele-specific forward primer represented by SEQ ID NO: 168, and the reverse primer set represented by SEQ ID NO: 168, the first allele-specific represented by SEQ ID NO: 169 for SK03_044 Forward primer, second allele-specific forward primer represented by SEQ ID NO: 170 and reverse primer set represented by SEQ ID NO: 171, first allele-specific forward primer represented by SEQ ID NO: 172 for SK03_050, SEQ ID NO: 173 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 174, a first allele-specific forward primer represented by SEQ ID NO: 175 for SK03_055, and a second allele-specific forward primer represented by SEQ ID NO: 176 and a reverse primer set represented by SEQ ID NO: 177, a first allele-specific forward primer represented by SEQ ID NO: 178 for SK03_063, a second allele-specific forward primer represented by SEQ ID NO: 179, and a reverse sequence represented by SEQ ID NO: 180. The anti-primer set, the first allele-specific forward primer represented by SEQ ID NO: 181 for SK03_070, the second allele-specific forward primer represented by SEQ ID NO: 182, and the reverse primer set represented by SEQ ID NO: 183, sequence for SK03_079 The first allele-specific forward primer represented by SEQ ID NO: 184, the second allele-specific forward primer represented by SEQ ID NO: 185, and the reverse primer set represented by SEQ ID NO: 186, the first allele represented by SEQ ID NO: 187 for SK03_094 Trait specific forward primer, second allele specific forward primer represented by SEQ ID NO: 188 and reverse primer set represented by SEQ ID NO: 189, first allele specific forward primer represented by SEQ ID NO: 190 for SK03_098, SEQ ID NO: 191 A second allele-specific forward primer represented by and a reverse primer set represented by SEQ ID NO: 192, a first allele-specific forward primer represented by SEQ ID NO: 193 for SK03_102, and a second allele-specific represented by SEQ ID NO: 194 A forward primer and a reverse primer set represented by SEQ ID NO: 195, a first allele-specific forward primer represented by SEQ ID NO: 196 for SK03_108, a second allele-specific forward primer represented by SEQ ID NO: 197, and SEQ ID NO: 198 A reverse primer set, the first allele-specific forward primer represented by SEQ ID NO: 199 for SK03_116, the second allele-specific forward primer represented by SEQ ID NO: 200, and the reverse primer set represented by SEQ ID NO: 201, SK03_120 The first allele-specific forward primer represented by SEQ ID NO: 202, the second allele-specific forward primer represented by SEQ ID NO: 203, and the reverse primer set represented by SEQ ID NO: 204, the first allele-specific forward primer represented by SEQ ID NO: 205 for SK03_126 1 allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 206 and the reverse primer set represented by SEQ ID NO: 207, the first allele-specific forward primer represented by SEQ ID NO: 208 for SK03_141, sequence The second allele-specific forward primer represented by No. 209 and the reverse primer set represented by SEQ ID NO: 210, the first allele-specific forward primer represented by SEQ ID NO: 211 for SK03_147, the second allele represented by SEQ ID NO: 212 A trait-specific forward primer and a set of reverse primers represented by SEQ ID NO: 213, a first allele-specific forward primer represented by SEQ ID NO: 214 for SK03_151, a second allele-specific forward primer represented by SEQ ID NO: 215, and SEQ ID NO: 216 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 217 for SK03_158, a second allele-specific forward primer represented by SEQ ID NO: 218, and a reverse primer set represented by SEQ ID NO: 219, A first allele-specific forward primer represented by SEQ ID NO: 220 for SK03_166, a second allele-specific forward primer represented by SEQ ID NO: 221 and a reverse primer set represented by SEQ ID NO: 222, SEQ ID NO: 223 for SK03_173 A set of first allele-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 224 and a reverse primer set represented by SEQ ID NO: 225, the first allele-specific forward primer represented by SEQ ID NO: 226 for SK03_180 , the second allele-specific forward primer represented by SEQ ID NO: 227 and the reverse primer set represented by SEQ ID NO: 228, the first allele-specific forward primer represented by SEQ ID NO: 229 for SK03_191, the first allele-specific forward primer represented by SEQ ID NO: 230 2 allele-specific forward primers and reverse primer set represented by SEQ ID NO: 231, the first allele-specific forward primer represented by SEQ ID NO: 232 for SK03_203, the second allele-specific forward primer represented by SEQ ID NO: 233, and the sequence Reverse primer set represented by No. 234, the first allele-specific forward primer represented by SEQ ID NO: 235 for SK03_219, the second allele-specific forward primer represented by SEQ ID NO: 236, and the reverse primer represented by SEQ ID NO: 237 Set, a first allele specific forward primer represented by SEQ ID NO: 238 for SK03_226, a second allele specific forward primer represented by SEQ ID NO: 239 and a reverse primer set represented by SEQ ID NO: 240, SEQ ID NO: 241 for SK03_237 The first allele-specific forward primer represented by , the second allele-specific forward primer represented by SEQ ID NO: 242, and the reverse primer set represented by SEQ ID NO: 243, the first allele-specific represented by SEQ ID NO: 244 for SK03_245 Forward primer, a second allele-specific forward primer represented by SEQ ID NO: 245 and a set of reverse primers represented by SEQ ID NO: 246, a first allele-specific forward primer represented by SEQ ID NO: 247 for SK03_252, SEQ ID NO: 248 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 249, a first allele-specific forward primer represented by SEQ ID NO: 250 for SK03_258, and a second allele-specific forward primer represented by SEQ ID NO: 251 and a set of reverse primers represented by SEQ ID NO: 252, a first allele-specific forward primer represented by SEQ ID NO: 253 for SK04_008, a second allele-specific forward primer represented by SEQ ID NO: 254, and a reverse sequence represented by SEQ ID NO: 255. The anti-primer set, the first allele-specific forward primer represented by SEQ ID NO: 256 for SK04_015, the second allele-specific forward primer represented by SEQ ID NO: 257, and the reverse primer set represented by SEQ ID NO: 258, sequence for SK04_023 The first allele-specific forward primer represented by SEQ ID NO: 259, the second allele-specific forward primer represented by SEQ ID NO: 260 and the reverse primer set represented by SEQ ID NO: 261, the first allele represented by SEQ ID NO: 262 for SK04_029 A set of trait-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 263 and a set of reverse primers represented by SEQ ID NO: 264, a first allele-specific forward primer represented by SEQ ID NO: 265 for SK04_045, SEQ ID NO: 266 A second allele-specific forward primer represented by and a reverse primer set represented by SEQ ID NO: 267, a first allele-specific forward primer represented by SEQ ID NO: 268 for SK04_051, and a second allele-specific represented by SEQ ID NO: 269 A forward primer and a reverse primer set represented by SEQ ID NO: 270, a first allele-specific forward primer represented by SEQ ID NO: 271 for SK04_060, a second allele-specific forward primer represented by SEQ ID NO: 272, and SEQ ID NO: 273 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 274 for SK04_069, a second allele-specific forward primer represented by SEQ ID NO: 275, and a reverse primer set represented by SEQ ID NO: 276, SK04_080 The first allele-specific forward primer represented by SEQ ID NO: 277, the second allele-specific forward primer represented by SEQ ID NO: 278, and the reverse primer set represented by SEQ ID NO: 279, the first allele-specific forward primer represented by SEQ ID NO: 280 for SK04_090 1 allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 281 and the reverse primer set represented by SEQ ID NO: 282, the first allele-specific forward primer represented by SEQ ID NO: 283 for SK04_098, sequence The second allele-specific forward primer represented by SEQ ID NO: 284 and the reverse primer set represented by SEQ ID NO: 285, the first allele-specific forward primer represented by SEQ ID NO: 286 for SK04_107, the second allele represented by SEQ ID NO: 287 A trait-specific forward primer and a set of reverse primers represented by SEQ ID NO: 288, a first allele-specific forward primer represented by SEQ ID NO: 289 for SK04_114, a second allele-specific forward primer represented by SEQ ID NO: 290, and SEQ ID NO: 291 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 292 for SK04_122, a second allele-specific forward primer represented by SEQ ID NO: 293, and a reverse primer set represented by SEQ ID NO: 294, A first allele-specific forward primer represented by SEQ ID NO: 295 for SK04_129, a second allele-specific forward primer represented by SEQ ID NO: 296 and a reverse primer set represented by SEQ ID NO: 297, SEQ ID NO: 298 for SK04_138 A first allele-specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 299, and a reverse primer set represented by SEQ ID NO: 300, the first allele-specific forward primer represented by SEQ ID NO: 301 for SK04_143 , the second allele-specific forward primer represented by SEQ ID NO: 302 and the reverse primer set represented by SEQ ID NO: 303, the first allele-specific forward primer represented by SEQ ID NO: 304 for SK04_149, the first allele-specific forward primer represented by SEQ ID NO: 305 2 allele-specific forward primers and reverse primer set represented by SEQ ID NO: 306, the first allele-specific forward primer represented by SEQ ID NO: 307 for SK04_158, the second allele-specific forward primer represented by SEQ ID NO: 308 and sequence Reverse primer set represented by No. 309, the first allele-specific forward primer represented by SEQ ID NO: 310 for SK04_164, the second allele-specific forward primer represented by SEQ ID NO: 311, and the reverse primer represented by SEQ ID NO: 312 Set, a first allele specific forward primer represented by SEQ ID NO: 313 to SK04_174, a second allele specific forward primer represented by SEQ ID NO: 314 and a reverse primer set represented by SEQ ID NO: 315, SEQ ID NO: 316 to SK04_180 The first allele-specific forward primer represented by , the second allele-specific forward primer represented by SEQ ID NO: 317, and the reverse primer set represented by SEQ ID NO: 318, the first allele-specific representation of SEQ ID NO: 319 for SK04_183 Forward primer, second allele-specific forward primer represented by SEQ ID NO: 320 and reverse primer set represented by SEQ ID NO: 321, first allele-specific forward primer represented by SEQ ID NO: 322 for SK04_189, SEQ ID NO: 323 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 324, a first allele-specific forward primer represented by SEQ ID NO: 325 for SK04_195, and a second allele-specific forward primer represented by SEQ ID NO: 326 and a reverse primer set represented by SEQ ID NO: 327, a first allele-specific forward primer represented by SEQ ID NO: 328 for SK04_200, a second allele-specific forward primer represented by SEQ ID NO: 329, and a reverse sequence represented by SEQ ID NO: 330. The anti-primer set, the first allele-specific forward primer represented by SEQ ID NO: 331 for SK04_204, the second allele-specific forward primer represented by SEQ ID NO: 332, and the reverse primer set represented by SEQ ID NO: 333, the sequence for SK05_003 The first allele-specific forward primer represented by SEQ ID NO: 334, the second allele-specific forward primer represented by SEQ ID NO: 335, and the reverse primer set represented by SEQ ID NO: 336, the first allele represented by SEQ ID NO: 337 for SK05_010 A trait-specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 338 and a set of reverse primers represented by SEQ ID NO: 339, a first allele-specific forward primer represented by SEQ ID NO: 340 for SK05_015, SEQ ID NO: 341 A second allele-specific forward primer represented by and a reverse primer set represented by SEQ ID NO: 342, a first allele-specific forward primer represented by SEQ ID NO: 343 for SK05_017, and a second allele-specific represented by SEQ ID NO: 344 A forward primer and a reverse primer set represented by SEQ ID NO: 345, a first allele-specific forward primer represented by SEQ ID NO: 346 for SK05_032, a second allele-specific forward primer represented by SEQ ID NO: 347, and SEQ ID NO: 348 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 349 for SK05_036, a second allele-specific forward primer represented by SEQ ID NO: 350, and a reverse primer set represented by SEQ ID NO: 351, SK05_039 The first allele-specific forward primer represented by SEQ ID NO: 352, the second allele-specific forward primer represented by SEQ ID NO: 353, and the reverse primer set represented by SEQ ID NO: 354, the first allele-specific forward primer represented by SEQ ID NO: 355 for SK05_059 1 allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 356 and the reverse primer set represented by SEQ ID NO: 357, the first allele-specific forward primer represented by SEQ ID NO: 358 for SK05_068, sequence The second allele-specific forward primer represented by No. 359 and the reverse primer set represented by SEQ ID NO: 360, the first allele-specific forward primer represented by SEQ ID NO: 361 for SK05_075, the second allele represented by SEQ ID NO: 362 A trait-specific forward primer and a set of reverse primers represented by SEQ ID NO: 363, a first allele-specific forward primer represented by SEQ ID NO: 364 for SK05_087, a second allele-specific forward primer represented by SEQ ID NO: 365, and SEQ ID NO: 366 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 367 for SK05_092, a second allele-specific forward primer represented by SEQ ID NO: 368, and a reverse primer set represented by SEQ ID NO: 369, A set of a first allele-specific forward primer represented by SEQ ID NO: 370 for SK05_100, a second allele-specific forward primer represented by SEQ ID NO: 371 and a reverse primer set represented by SEQ ID NO: 372, SEQ ID NO: 373 for SK05_108 A first allele-specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 374, and a reverse primer set represented by SEQ ID NO: 375, the first allele-specific forward primer represented by SEQ ID NO: 374 for SK05_112 , the second allele-specific forward primer represented by SEQ ID NO: 375 and the reverse primer set represented by SEQ ID NO: 376, the first allele-specific forward primer represented by SEQ ID NO: 379 for SK05_115, the first allele-specific forward primer represented by SEQ ID NO: 380 2 allele-specific forward primers and reverse primer set represented by SEQ ID NO: 381, the first allele-specific forward primer represented by SEQ ID NO: 382 for SK05_122, the second allele-specific forward primer represented by SEQ ID NO: 383, and the sequence Reverse primer set represented by No. 384, the first allele-specific forward primer represented by SEQ ID NO: 385 for SK05_132, the second allele-specific forward primer represented by SEQ ID NO: 386, and the reverse primer represented by SEQ ID NO: 387 Set, a first allele specific forward primer represented by SEQ ID NO: 388 to SK05_137, a second allele specific forward primer represented by SEQ ID NO: 389 and a reverse primer set represented by SEQ ID NO: 390, SEQ ID NO: 391 to SK05_148 The first allele-specific forward primer represented by SEQ ID NO: 392, the second allele-specific forward primer represented by SEQ ID NO: 393, and the reverse primer set represented by SEQ ID NO: 394 for SK05_155. Forward primer, second allele-specific forward primer represented by SEQ ID NO: 395 and reverse primer set represented by SEQ ID NO: 396, first allele-specific forward primer represented by SEQ ID NO: 397 for SK05_162, SEQ ID NO: 398 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 399, a first allele-specific forward primer represented by SEQ ID NO: 400 for SK06_006, and a second allele-specific forward primer represented by SEQ ID NO: 401 and a set of reverse primers represented by SEQ ID NO: 402, a first allele-specific forward primer represented by SEQ ID NO: 403 for SK06_013, a second allele-specific forward primer represented by SEQ ID NO: 404, and a reverse sequence represented by SEQ ID NO: 405. The anti-primer set, the first allele-specific forward primer represented by SEQ ID NO: 406 for SK06_022, the second allele-specific forward primer represented by SEQ ID NO: 407, and the reverse primer set represented by SEQ ID NO: 408, the sequence for SK06_029 The first allele-specific forward primer represented by SEQ ID NO: 409, the second allele-specific forward primer represented by SEQ ID NO: 410, and the reverse primer set represented by SEQ ID NO: 411, the first allele represented by SEQ ID NO: 412 for SK06_035 Trait specific forward primer, second allele specific forward primer represented by SEQ ID NO: 413 and reverse primer set represented by SEQ ID NO: 414, first allele specific forward primer represented by SEQ ID NO: 415 for SK06_042, SEQ ID NO: 416 A second allele-specific forward primer represented by and a reverse primer set represented by SEQ ID NO: 417, a first allele-specific forward primer represented by SEQ ID NO: 418 for SK06_048, and a second allele-specific represented by SEQ ID NO: 419 A forward primer and a reverse primer set represented by SEQ ID NO: 420, a first allele-specific forward primer represented by SEQ ID NO: 421 for SK06_056, a second allele-specific forward primer represented by SEQ ID NO: 422, and SEQ ID NO: 423 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 424 for SK06_063, a second allele-specific forward primer represented by SEQ ID NO: 425, and a reverse primer set represented by SEQ ID NO: 426, SK06_067 The first allele-specific forward primer represented by SEQ ID NO: 427, the second allele-specific forward primer represented by SEQ ID NO: 428, and the reverse primer set represented by SEQ ID NO: 429, the first allele-specific forward primer represented by SEQ ID NO: 430 for SK06_075 1 allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 431 and the reverse primer set represented by SEQ ID NO: 432, the first allele-specific forward primer represented by SEQ ID NO: 433 for SK06_081, sequence The second allele-specific forward primer represented by No. 434 and the reverse primer set represented by SEQ ID NO: 435, the first allele-specific forward primer represented by SEQ ID NO: 436 for SK06_098, the second allele represented by SEQ ID NO: 437 A trait-specific forward primer and a set of reverse primers represented by SEQ ID NO: 438, a first allele-specific forward primer represented by SEQ ID NO: 439 for SK06_105, a second allele-specific forward primer represented by SEQ ID NO: 440, and SEQ ID NO: 441 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 442 for SK06_113, a second allele-specific forward primer represented by SEQ ID NO: 443, and a reverse primer set represented by SEQ ID NO: 444, A first allele-specific forward primer represented by SEQ ID NO: 445 for SK06_121, a second allele-specific forward primer represented by SEQ ID NO: 446 and a reverse primer set represented by SEQ ID NO: 447, SEQ ID NO: 448 for SK06_129 A first allele-specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 449 and a reverse primer set represented by SEQ ID NO: 450, the first allele-specific forward primer represented by SEQ ID NO: 451 for SK06_136 , the second allele-specific forward primer represented by SEQ ID NO: 452 and the reverse primer set represented by SEQ ID NO: 453, the first allele-specific forward primer represented by SEQ ID NO: 454 for SK06_139, the first allele-specific forward primer represented by SEQ ID NO: 455 2 allele-specific forward primers and reverse primer set represented by SEQ ID NO: 456, the first allele-specific forward primer represented by SEQ ID NO: 457 for SK06_145, the second allele-specific forward primer represented by SEQ ID NO: 458 and the sequence Reverse primer set represented by No. 459, the first allele-specific forward primer represented by SEQ ID NO: 460 for SK06_152, the second allele-specific forward primer represented by SEQ ID NO: 461, and the reverse primer represented by SEQ ID NO: 462 Set, a first allele specific forward primer represented by SEQ ID NO: 463 for SK06_157, a second allele specific forward primer represented by SEQ ID NO: 464 and a reverse primer set represented by SEQ ID NO: 465, SEQ ID NO: 466 for SK06_162 The first allele-specific forward primer represented by , the second allele-specific forward primer represented by SEQ ID NO: 467 and the reverse primer set represented by SEQ ID NO: 468, the first allele-specific represented by SEQ ID NO: 469 for SK06_170 Forward primer, a second allele-specific forward primer represented by SEQ ID NO: 470 and a set of reverse primers represented by SEQ ID NO: 471, a first allele-specific forward primer represented by SEQ ID NO: 472 for SK06_177, SEQ ID NO: 473 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 474, a first allele-specific forward primer represented by SEQ ID NO: 475 for SK06_183, and a second allele-specific forward primer represented by SEQ ID NO: 476 and a set of reverse primers represented by SEQ ID NO: 477, a first allele-specific forward primer represented by SEQ ID NO: 478 for SK06_190, a second allele-specific forward primer represented by SEQ ID NO: 479, and a reverse sequence represented by SEQ ID NO: 480. The anti-primer set, the first allele-specific forward primer represented by SEQ ID NO: 481 for SK06_191, the second allele-specific forward primer represented by SEQ ID NO: 482, and the reverse primer set represented by SEQ ID NO: 483, the sequence for SK06_198 The first allele-specific forward primer represented by SEQ ID NO: 484, the second allele-specific forward primer represented by SEQ ID NO: 485, and the reverse primer set represented by SEQ ID NO: 486, the first allele represented by SEQ ID NO: 487 for SK06_205 Trait specific forward primer, second allele specific forward primer represented by SEQ ID NO: 488 and reverse primer set represented by SEQ ID NO: 489, first allele specific forward primer represented by SEQ ID NO: 490 for SK06_212, SEQ ID NO: 491 A second allele-specific forward primer represented by and a reverse primer set represented by SEQ ID NO: 492, a first allele-specific forward primer represented by SEQ ID NO: 493 for SK06_222, and a second allele-specific represented by SEQ ID NO: 494 A forward primer and a reverse primer set represented by SEQ ID NO: 495, a first allele-specific forward primer represented by SEQ ID NO: 496 for SK06_235, a second allele-specific forward primer represented by SEQ ID NO: 497, and SEQ ID NO: 498 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 499 for SK06_240, a second allele-specific forward primer represented by SEQ ID NO: 500, and a reverse primer set represented by SEQ ID NO: 501, SK06_249 The first allele-specific forward primer represented by SEQ ID NO: 502, the second allele-specific forward primer represented by SEQ ID NO: 503, and the reverse primer set represented by SEQ ID NO: 504, the first allele-specific forward primer represented by SEQ ID NO: 505 for SK06_256 1 allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 506 and the reverse primer set represented by SEQ ID NO: 507, the first allele-specific forward primer represented by SEQ ID NO: 508 for SK07_006, sequence The second allele-specific forward primer represented by No. 509 and the reverse primer set represented by SEQ ID NO: 510, the first allele-specific forward primer represented by SEQ ID NO: 511 for SK07_022, the second allele represented by SEQ ID NO: 512 A trait-specific forward primer and a set of reverse primers represented by SEQ ID NO: 513, a first allele-specific forward primer represented by SEQ ID NO: 514 for SK07_043, a second allele-specific forward primer represented by SEQ ID NO: 515, and SEQ ID NO: 516 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 517 for SK07_047, a second allele-specific forward primer represented by SEQ ID NO: 518, and a reverse primer set represented by SEQ ID NO: 519, A set of a first allele-specific forward primer represented by SEQ ID NO: 520 for SK07_055, a second allele-specific forward primer represented by SEQ ID NO: 521 and a reverse primer set represented by SEQ ID NO: 522, SEQ ID NO: 523 for SK07_064 A first allele-specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 524, and a reverse primer set represented by SEQ ID NO: 525, the first allele-specific forward primer represented by SEQ ID NO: 526 for SK07_073 , the second allele-specific forward primer represented by SEQ ID NO: 527 and the reverse primer set represented by SEQ ID NO: 528, the first allele-specific forward primer represented by SEQ ID NO: 529 for SK07_079, the first allele-specific forward primer represented by SEQ ID NO: 530 2 allele-specific forward primers and reverse primer set represented by SEQ ID NO: 531, the first allele-specific forward primer represented by SEQ ID NO: 532 for SK07_083, the second allele-specific forward primer represented by SEQ ID NO: 533 and sequence Reverse primer set represented by No. 534, the first allele-specific forward primer represented by SEQ ID NO: 535 for SK07_094, the second allele-specific forward primer represented by SEQ ID NO: 536, and the reverse primer represented by SEQ ID NO: 537 Set, a first allele specific forward primer represented by SEQ ID NO: 538 for SK07_100, a second allele specific forward primer represented by SEQ ID NO: 539 and a reverse primer set represented by SEQ ID NO: 540, SEQ ID NO: 541 for SK07_106 The first allele-specific forward primer represented by , the second allele-specific forward primer represented by SEQ ID NO: 542 and the reverse primer set represented by SEQ ID NO: 543, the first allele-specific forward primer represented by SEQ ID NO: 544 for SK07_114 Primers, the second allele-specific forward primer represented by SEQ ID NO: 545 and the reverse primer set represented by SEQ ID NO: 546, the first allele-specific forward primer represented by SEQ ID NO: 547 for SK07_126, SEQ ID NO: 548 A second allele-specific forward primer and a set of reverse primers represented by SEQ ID NO: 549, a first allele-specific forward primer represented by SEQ ID NO: 550 for SK07_133, a second allele-specific forward primer represented by SEQ ID NO: 551, and A reverse primer set represented by SEQ ID NO: 552, a first allele-specific forward primer represented by SEQ ID NO: 553 for SK07_130, a second allele-specific forward primer represented by SEQ ID NO: 554, and a reverse primer represented by SEQ ID NO: 555 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 556 for SK07_145, a second allele-specific forward primer represented by SEQ ID NO: 557, and a reverse primer set represented by SEQ ID NO: 558, SEQ ID NO: 551 The first allele-specific forward primer represented by SEQ ID NO: 559, the second allele-specific forward primer represented by SEQ ID NO: 560, and the reverse primer set represented by SEQ ID NO: 561, the first allele represented by SEQ ID NO: 562 for SK07_164 A specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 563 and a set of reverse primers represented by SEQ ID NO: 564, a first allele-specific forward primer represented by SEQ ID NO: 565 for SK07_166, SEQ ID NO: 566 The second allele-specific forward primer and the reverse primer set represented by SEQ ID NO: 567, the first allele-specific forward primer represented by SEQ ID NO: 568 for SK08_001, and the second allele-specific forward primer represented by SEQ ID NO: 569 Primers and a set of reverse primers represented by SEQ ID NO: 570, a first allele-specific forward primer represented by SEQ ID NO: 571 for SK08_008, a second allele-specific forward primer represented by SEQ ID NO: 572, and SEQ ID NO: 573 The reverse primer set, the first allele-specific forward primer represented by SEQ ID NO: 574 for SK08_012, the second allele-specific forward primer represented by SEQ ID NO: 575, and the reverse primer set represented by SEQ ID NO: 576, for SK08_020 A set of first allele-specific forward primers represented by SEQ ID NO: 577, second allele-specific forward primers represented by SEQ ID NO: 578 and reverse primers represented by SEQ ID NO: 579, the first set represented by SEQ ID NO: 580 for SK08_032 Allele-specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 581 and a reverse primer set represented by SEQ ID NO: 582, a first allele-specific forward primer represented by SEQ ID NO: 583 for SK08_039, SEQ ID NO: The second allele-specific forward primer represented by SEQ ID NO: 584 and the reverse primer set represented by SEQ ID NO: 585, the first allele-specific forward primer represented by SEQ ID NO: 586 for SK08_046, the second allele represented by SEQ ID NO: 587 A specific forward primer and a set of reverse primers represented by SEQ ID NO: 588, a first allele-specific forward primer represented by SEQ ID NO: 589 for SK08_052, a second allele-specific forward primer represented by SEQ ID NO: 590, and SEQ ID NO: 591 The reverse primer set represented by, the first allele-specific forward primer represented by SEQ ID NO: 592 for SK08_066, the second allele-specific forward primer represented by SEQ ID NO: 593, and the reverse primer set represented by SEQ ID NO: 594, SK08_072 The first allele-specific forward primer represented by SEQ ID NO: 595, the second allele-specific forward primer represented by SEQ ID NO: 596, and the reverse primer set represented by SEQ ID NO: 597, SEQ ID NO: 598 for SK08_125 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 599 and the reverse primer set represented by SEQ ID NO: 600, the first allele-specific forward primer represented by SEQ ID NO: 601 for SK08_120, The second allele-specific forward primer represented by SEQ ID NO: 602 and the reverse primer set represented by SEQ ID NO: 603, the first allele-specific forward primer represented by SEQ ID NO: 604 for SK08_137, the second allele-specific forward primer represented by SEQ ID NO: 605 Allele-specific forward primer and reverse primer set represented by SEQ ID NO: 606, first allele-specific forward primer represented by SEQ ID NO: 607 for SK08_142, second allele-specific forward primer represented by SEQ ID NO: 608 and SEQ ID NO: A reverse primer set represented by SEQ ID NO: 609, a first allele-specific forward primer represented by SEQ ID NO: 610 for SK08_148, a second allele-specific forward primer represented by SEQ ID NO: 611, and a reverse primer set represented by SEQ ID NO: 612 , the first allele-specific forward primer represented by SEQ ID NO: 613 for SK08_156, the second allele-specific forward primer represented by SEQ ID NO: 614 and the reverse primer set represented by SEQ ID NO: 615, SEQ ID NO: 616 for SK08_164 The first allele-specific forward primer represented by SEQ ID NO: 617, the second allele-specific forward primer represented by SEQ ID NO: 618, and the set of reverse primers represented by SEQ ID NO: 618, the first allele-specific forward primer represented by SEQ ID NO: 619 for SK08_172 Primers, a second allele-specific forward primer represented by SEQ ID NO: 620 and a reverse primer set represented by SEQ ID NO: 621, a first allele-specific forward primer represented by SEQ ID NO: 622 for SK08_179, SEQ ID NO: 623 A second allele-specific forward primer and a set of reverse primers represented by SEQ ID NO: 624, a first allele-specific forward primer represented by SEQ ID NO: 625 for SK08_187, a second allele-specific forward primer represented by SEQ ID NO: 626, and A reverse primer set represented by SEQ ID NO: 627, a first allele-specific forward primer represented by SEQ ID NO: 628 for SK08_197, a second allele-specific forward primer represented by SEQ ID NO: 629, and a reverse primer represented by SEQ ID NO: 630 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 631 for SK08_203, a second allele-specific forward primer represented by SEQ ID NO: 632, and a reverse primer set represented by SEQ ID NO: 633, SEQ ID NO: A set of first allele specific forward primers represented by SEQ ID NO: 634, second allele specific forward primers represented by SEQ ID NO: 635 and reverse primers represented by SEQ ID NO: 636, first allele represented by SEQ ID NO: 637 for SK08_216 A specific forward primer, a second allele specific forward primer represented by SEQ ID NO: 638 and a set of reverse primers represented by SEQ ID NO: 639, a first allele specific forward primer represented by SEQ ID NO: 640 for SK08_219, SEQ ID NO: 641 The second allele-specific forward primer and the reverse primer set represented by SEQ ID NO: 642, the first allele-specific forward primer represented by SEQ ID NO: 643 for SK08_227, and the second allele-specific forward primer represented by SEQ ID NO: 644 Primers and a set of reverse primers represented by SEQ ID NO: 645, a first allele-specific forward primer represented by SEQ ID NO: 646 for SK08_233, a second allele-specific forward primer represented by SEQ ID NO: 647, and SEQ ID NO: 648 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 649 for SK08_241, a second allele-specific forward primer represented by SEQ ID NO: 650, and a reverse primer set represented by SEQ ID NO: 651, for SK08_254 A set of first allele-specific forward primers represented by SEQ ID NO: 652, second allele-specific forward primers represented by SEQ ID NO: 653 and reverse primers represented by SEQ ID NO: 654, the first set represented by SEQ ID NO: 655 for SK08_260 Allele-specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 656 and a set of reverse primers represented by SEQ ID NO: 657, a first allele-specific forward primer represented by SEQ ID NO: 658 for SK09_004, SEQ ID NO: The second allele-specific forward primer represented by SEQ ID NO: 659 and the reverse primer set represented by SEQ ID NO: 660, the first allele-specific forward primer represented by SEQ ID NO: 661 for SK09_028, the second allele represented by SEQ ID NO: 662 A specific forward primer and a set of reverse primers represented by SEQ ID NO: 663, a first allele-specific forward primer represented by SEQ ID NO: 664 for SK09_035, a second allele-specific forward primer represented by SEQ ID NO: 665 and SEQ ID NO: 666 The reverse primer set represented by, the first allele-specific forward primer represented by SEQ ID NO: 667 for SK09_042, the second allele-specific forward primer represented by SEQ ID NO: 668, and the reverse primer set represented by SEQ ID NO: 669, SK09_052 The first allele-specific forward primer represented by SEQ ID NO: 670, the second allele-specific forward primer represented by SEQ ID NO: 671, and the reverse primer set represented by SEQ ID NO: 672, SEQ ID NO: 673 for SK09_056 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 674 and the reverse primer set represented by SEQ ID NO: 675, the first allele-specific forward primer represented by SEQ ID NO: 676 for SK09_062, The second allele-specific forward primer represented by SEQ ID NO: 677 and the reverse primer set represented by SEQ ID NO: 678, the first allele-specific forward primer represented by SEQ ID NO: 679 for SK09_068, the second allele-specific forward primer represented by SEQ ID NO: 680 Allele-specific forward primer and reverse primer set represented by SEQ ID NO: 681, the first allele-specific forward primer represented by SEQ ID NO: 682 for SK09_075, the second allele-specific forward primer represented by SEQ ID NO: 683 and SEQ ID NO: A reverse primer set represented by SEQ ID NO: 684, a first allele-specific forward primer represented by SEQ ID NO: 685 for SK09_093, a second allele-specific forward primer represented by SEQ ID NO: 686, and a reverse primer set represented by SEQ ID NO: 687 , the first allele-specific forward primer represented by SEQ ID NO: 688 for SK09_123, the second allele-specific forward primer represented by SEQ ID NO: 689 and the reverse primer set represented by SEQ ID NO: 690, SEQ ID NO: 691 for SK09_132 The first allele-specific forward primer represented by SEQ ID NO: 692, the second allele-specific forward primer represented by SEQ ID NO: 693, and the set of reverse primers represented by SEQ ID NO: 693, the first allele-specific forward primer represented by SEQ ID NO: 694 for SK09_140 Primers, a second allele-specific forward primer represented by SEQ ID NO: 695 and a reverse primer set represented by SEQ ID NO: 696, a first allele-specific forward primer represented by SEQ ID NO: 697 for SK09_154, SEQ ID NO: 698 A second allele-specific forward primer and a set of reverse primers represented by SEQ ID NO: 699, a first allele-specific forward primer represented by SEQ ID NO: 700 for SK09_162, a second allele-specific forward primer represented by SEQ ID NO: 701, and A reverse primer set represented by SEQ ID NO: 702, a first allele-specific forward primer represented by SEQ ID NO: 703 for SK09_160, a second allele-specific forward primer represented by SEQ ID NO: 704, and a reverse primer represented by SEQ ID NO: 705 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 706 for SK09_169, a second allele-specific forward primer represented by SEQ ID NO: 707, and a reverse primer set represented by SEQ ID NO: 708, SEQ ID NO: 709_173 The first allele-specific forward primer represented by SEQ ID NO: 709, the second allele-specific forward primer represented by SEQ ID NO: 710, and the reverse primer set represented by SEQ ID NO: 711, the first allele represented by SEQ ID NO: 712 for SK09_177 A specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 713 and a set of reverse primers represented by SEQ ID NO: 714, a first allele-specific forward primer represented by SEQ ID NO: 715 for SK09_181, SEQ ID NO: 716 The second allele-specific forward primer and the reverse primer set represented by SEQ ID NO: 717, the first allele-specific forward primer represented by SEQ ID NO: 718 for SK09_186, and the second allele-specific forward primer represented by SEQ ID NO: 719 Primers and a set of reverse primers represented by SEQ ID NO: 720, a first allele-specific forward primer represented by SEQ ID NO: 721 for SK09_189, a second allele-specific forward primer represented by SEQ ID NO: 722, and SEQ ID NO: 723 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 724 for SK09_197, a second allele-specific forward primer represented by SEQ ID NO: 725, and a reverse primer set represented by SEQ ID NO: 726, for SK09_208 A set of first allele-specific forward primers represented by SEQ ID NO: 727, second allele-specific forward primers represented by SEQ ID NO: 728 and reverse primers represented by SEQ ID NO: 729, the first set represented by SEQ ID NO: 730 for SK09_213 Allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 731 and the set of reverse primers represented by SEQ ID NO: 732, the first allele-specific forward primer represented by SEQ ID NO: 733 for SK09_224, SEQ ID NO: The second allele-specific forward primer represented by SEQ ID NO: 734 and the reverse primer set represented by SEQ ID NO: 735, the first allele-specific forward primer represented by SEQ ID NO: 736 for SK09_228, the second allele represented by SEQ ID NO: 737 A specific forward primer and a set of reverse primers represented by SEQ ID NO: 738, a first allele-specific forward primer represented by SEQ ID NO: 739 for SK10_005, a second allele-specific forward primer represented by SEQ ID NO: 740 and SEQ ID NO: 741 The reverse primer set represented by, the first allele-specific forward primer represented by SEQ ID NO: 742 for SK10_011, the second allele-specific forward primer represented by SEQ ID NO: 743, and the reverse primer set represented by SEQ ID NO: 744, SK10_014 The first allele-specific forward primer represented by SEQ ID NO: 745, the second allele-specific forward primer represented by SEQ ID NO: 746, and the reverse primer set represented by SEQ ID NO: 747, SEQ ID NO: 748 for SK10_019 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 749 and the reverse primer set represented by SEQ ID NO: 750, the first allele-specific forward primer represented by SEQ ID NO: 751 for SK10_028, The second allele-specific forward primer represented by SEQ ID NO: 752 and the reverse primer set represented by SEQ ID NO: 753, the first allele-specific forward primer represented by SEQ ID NO: 754 for SK10_032, the second allele-specific forward primer represented by SEQ ID NO: 755 Allele-specific forward primer and reverse primer set represented by SEQ ID NO: 756, first allele-specific forward primer represented by SEQ ID NO: 757 for SK10_038, second allele-specific forward primer represented by SEQ ID NO: 758 and SEQ ID NO: A reverse primer set represented by 759, a first allele-specific forward primer represented by SEQ ID NO: 760 for SK10_046, a second allele-specific forward primer represented by SEQ ID NO: 761, and a reverse primer set represented by SEQ ID NO: 762 , the first allele-specific forward primer represented by SEQ ID NO: 763 for SK10_053, the second allele-specific forward primer represented by SEQ ID NO: 764 and the reverse primer set represented by SEQ ID NO: 765, SEQ ID NO: 766 for SK10_061 A set of first allele specific forward primers represented by SEQ ID NO: 757, second allele specific forward primers represented by SEQ ID NO: 768 and reverse primer set represented by SEQ ID NO: 768, first allele specific forward primers represented by SEQ ID NO: 769 for SK10_076 Primers, the second allele-specific forward primer represented by SEQ ID NO: 770 and the reverse primer set represented by SEQ ID NO: 771, the first allele-specific forward primer represented by SEQ ID NO: 772 for SK10_086, SEQ ID NO: 773 A second allele-specific forward primer and a set of reverse primers represented by SEQ ID NO: 774, a first allele-specific forward primer represented by SEQ ID NO: 775 for SK10_092, a second allele-specific forward primer represented by SEQ ID NO: 776, and A reverse primer set represented by SEQ ID NO: 777, a first allele-specific forward primer represented by SEQ ID NO: 778 for SK10_097, a second allele-specific forward primer represented by SEQ ID NO: 779, and a reverse primer represented by SEQ ID NO: 780 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 781 for SK10_113, a second allele-specific forward primer represented by SEQ ID NO: 782, and a reverse primer set represented by SEQ ID NO: 783, SEQ ID NO: 783 A set of first allele specific forward primers represented by SEQ ID NO: 784, second allele specific forward primers represented by SEQ ID NO: 785 and reverse primers represented by SEQ ID NO: 786, first allele represented by SEQ ID NO: 787 for SK10_128 A specific forward primer, a second allele specific forward primer represented by SEQ ID NO: 788 and a set of reverse primers represented by SEQ ID NO: 789, a first allele specific forward primer represented by SEQ ID NO: 790 for SK10_131, SEQ ID NO: 791 The second allele-specific forward primer and the reverse primer set represented by SEQ ID NO: 792, the first allele-specific forward primer represented by SEQ ID NO: 793 for SK10_135, and the second allele-specific forward primer represented by SEQ ID NO: 794 Primers and a set of reverse primers represented by SEQ ID NO: 795, a first allele-specific forward primer represented by SEQ ID NO: 796 for SK10_146, a second allele-specific forward primer represented by SEQ ID NO: 797, and SEQ ID NO: 798 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 799 for SK10_153, a second allele-specific forward primer represented by SEQ ID NO: 800, and a reverse primer set represented by SEQ ID NO: 801, for SK10_161 A set of first allele-specific forward primers represented by SEQ ID NO: 802, second allele-specific forward primers represented by SEQ ID NO: 803 and reverse primers represented by SEQ ID NO: 804, the first set represented by SEQ ID NO: 805 for SK10_167 Allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 806 and the reverse primer set represented by SEQ ID NO: 807, the first allele-specific forward primer represented by SEQ ID NO: 808 for SK10_174, SEQ ID NO: The second allele-specific forward primer represented by SEQ ID NO: 809 and the reverse primer set represented by SEQ ID NO: 810, the first allele-specific forward primer represented by SEQ ID NO: 811 for SK10_182, the second allele represented by SEQ ID NO: 812 A set of specific forward primers and reverse primers represented by SEQ ID NO: 813, a first allele-specific forward primer represented by SEQ ID NO: 814 for SK11_001, a second allele-specific forward primer represented by SEQ ID NO: 815, and SEQ ID NO: 816 The reverse primer set represented by, the first allele-specific forward primer represented by SEQ ID NO: 817 for SK11_007, the second allele-specific forward primer represented by SEQ ID NO: 818, and the reverse primer set represented by SEQ ID NO: 819, SK11_015 The first allele-specific forward primer represented by SEQ ID NO: 820, the second allele-specific forward primer represented by SEQ ID NO: 821, and the reverse primer set represented by SEQ ID NO: 822, SEQ ID NO: 823 for SK11_022 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 824 and the reverse primer set represented by SEQ ID NO: 825, the first allele-specific forward primer represented by SEQ ID NO: 826 for SK11_027, The second allele-specific forward primer represented by SEQ ID NO: 827 and the reverse primer set represented by SEQ ID NO: 828, the first allele-specific forward primer represented by SEQ ID NO: 829 for SK11_035, the second allele-specific forward primer represented by SEQ ID NO: 830 Allele-specific forward primer and reverse primer set represented by SEQ ID NO: 831, first allele-specific forward primer represented by SEQ ID NO: 832 for SK11_042, second allele-specific forward primer represented by SEQ ID NO: 833 and SEQ ID NO: A reverse primer set represented by SEQ ID NO: 834, a first allele-specific forward primer represented by SEQ ID NO: 835 for SK11_050, a second allele-specific forward primer represented by SEQ ID NO: 836, and a reverse primer set represented by SEQ ID NO: 837 , the first allele-specific forward primer represented by SEQ ID NO: 838 for SK11_059, the second allele-specific forward primer represented by SEQ ID NO: 839 and the reverse primer set represented by SEQ ID NO: 840, SEQ ID NO: 841 for SK11_066 The first allele-specific forward primer represented by SEQ ID NO: 842, the second allele-specific forward primer represented by SEQ ID NO: 843, and the set of reverse primers represented by SEQ ID NO: 843, the first allele-specific forward primer represented by SEQ ID NO: 844 for SK11_073 A set of primers, a second allele-specific forward primer represented by SEQ ID NO: 845 and a reverse primer set represented by SEQ ID NO: 846, a first allele-specific forward primer represented by SEQ ID NO: 847 for SK11_084, SEQ ID NO: 848 A second allele-specific forward primer and a set of reverse primers represented by SEQ ID NO: 849, a first allele-specific forward primer represented by SEQ ID NO: 850 for SK11_092, a second allele-specific forward primer represented by SEQ ID NO: 851, and A reverse primer set represented by SEQ ID NO: 852, a first allele-specific forward primer represented by SEQ ID NO: 853 for SK11_102, a second allele-specific forward primer represented by SEQ ID NO: 854, and a reverse primer represented by SEQ ID NO: 855 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 856 for SK11_109, a second allele-specific forward primer represented by SEQ ID NO: 857, and a reverse primer set represented by SEQ ID NO: 858, SEQ ID NO: SK11_117 The first allele-specific forward primer represented by SEQ ID NO: 859, the second allele-specific forward primer represented by SEQ ID NO: 860, and the reverse primer set represented by SEQ ID NO: 861, the first allele represented by SEQ ID NO: 862 for SK11_125 A specific forward primer, a second allele specific forward primer represented by SEQ ID NO: 863 and a set of reverse primers represented by SEQ ID NO: 864, a first allele specific forward primer represented by SEQ ID NO: 865 for SK11_130, SEQ ID NO: 866 The second allele-specific forward primer and the reverse primer set represented by SEQ ID NO: 867, the first allele-specific forward primer represented by SEQ ID NO: 868 for SK11_137, and the second allele-specific forward primer represented by SEQ ID NO: 869 Primers and a set of reverse primers represented by SEQ ID NO: 870, a first allele-specific forward primer represented by SEQ ID NO: 871 for SK12_007, a second allele-specific forward primer represented by SEQ ID NO: 872, and SEQ ID NO: 873 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 874 for SK12_019, a second allele-specific forward primer represented by SEQ ID NO: 875, and a reverse primer set represented by SEQ ID NO: 876, for SK12_022 A set of first allele-specific forward primers represented by SEQ ID NO: 877, second allele-specific forward primers represented by SEQ ID NO: 878 and reverse primers represented by SEQ ID NO: 879, the first set represented by SEQ ID NO: 880 for SK12_033 Allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 881 and the set of reverse primers represented by SEQ ID NO: 882, the first allele-specific forward primer represented by SEQ ID NO: 883 for SK12_042, SEQ ID NO: The second allele-specific forward primer represented by SEQ ID NO: 884 and the reverse primer set represented by SEQ ID NO: 885, the first allele-specific forward primer represented by SEQ ID NO: 886 for SK12_052, the second allele represented by SEQ ID NO: 887 A specific forward primer and a set of reverse primers represented by SEQ ID NO: 888, a first allele-specific forward primer represented by SEQ ID NO: 889 for SK12_066, a second allele-specific forward primer represented by SEQ ID NO: 890 and SEQ ID NO: 891 The reverse primer set represented by, the first allele-specific forward primer represented by SEQ ID NO: 892 for SK12_073, the second allele-specific forward primer represented by SEQ ID NO: 893, and the reverse primer set represented by SEQ ID NO: 894, SK12_080 The first allele-specific forward primer represented by SEQ ID NO: 895, the second allele-specific forward primer represented by SEQ ID NO: 896, and the reverse primer set represented by SEQ ID NO: 897, SEQ ID NO: 898 for SK12_089 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 899 and the reverse primer set represented by SEQ ID NO: 900, the first allele-specific forward primer represented by SEQ ID NO: 901 for SK12_096, The second allele-specific forward primer represented by SEQ ID NO: 902 and the reverse primer set represented by SEQ ID NO: 903, the first allele-specific forward primer represented by SEQ ID NO: 904 for SK12_110, and the second allele-specific forward primer represented by SEQ ID NO: 905 Allele-specific forward primer and reverse primer set represented by SEQ ID NO: 906, first allele-specific forward primer represented by SEQ ID NO: 907 for SK12_118, second allele-specific forward primer represented by SEQ ID NO: 908 and SEQ ID NO: A reverse primer set represented by 909, a first allele-specific forward primer represented by SEQ ID NO: 910 for SK12_123, a second allele-specific forward primer represented by SEQ ID NO: 911, and a reverse primer set represented by SEQ ID NO: 912 , the first allele-specific forward primer represented by SEQ ID NO: 913 for SK12_128, the second allele-specific forward primer represented by SEQ ID NO: 914 and the reverse primer set represented by SEQ ID NO: 915, SEQ ID NO: 916 for SK12_134 The first allele-specific forward primer represented by SEQ ID NO: 917, the second allele-specific forward primer represented by SEQ ID NO: 918, and the set of reverse primers represented by SEQ ID NO: 918, the first allele-specific forward primer represented by SEQ ID NO: 919 for SK12_138 Primers, the second allele-specific forward primer represented by SEQ ID NO: 920 and the reverse primer set represented by SEQ ID NO: 921, the first allele-specific forward primer represented by SEQ ID NO: 922 for SK12_147, SEQ ID NO: 923 A second allele-specific forward primer and a set of reverse primers represented by SEQ ID NO: 924, a first allele-specific forward primer represented by SEQ ID NO: 925 for SK12_151, a second allele-specific forward primer represented by SEQ ID NO: 926, and A reverse primer set represented by SEQ ID NO: 927, a first allele-specific forward primer represented by SEQ ID NO: 928 for SK12_161, a second allele-specific forward primer represented by SEQ ID NO: 929, and a reverse primer represented by SEQ ID NO: 930 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 931 for SK13_003, a second allele-specific forward primer represented by SEQ ID NO: 932, and a reverse primer set represented by SEQ ID NO: 933, SEQ ID NO: SK13_008 The first allele-specific forward primer represented by SEQ ID NO: 934, the second allele-specific forward primer represented by SEQ ID NO: 935, and the reverse primer set represented by SEQ ID NO: 936, the first allele represented by SEQ ID NO: 937 for SK13_015 A specific forward primer, a second allele specific forward primer represented by SEQ ID NO: 938 and a set of reverse primers represented by SEQ ID NO: 939, a first allele specific forward primer represented by SEQ ID NO: 940 for SK13_023, SEQ ID NO: 941 The second allele-specific forward primer and the reverse primer set represented by SEQ ID NO: 942, the first allele-specific forward primer represented by SEQ ID NO: 943 for SK13_055, and the second allele-specific forward primer represented by SEQ ID NO: 944 Primers and a set of reverse primers represented by SEQ ID NO: 945, a first allele-specific forward primer represented by SEQ ID NO: 946 for SK13_061, a second allele-specific forward primer represented by SEQ ID NO: 947, and SEQ ID NO: 948 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 949 for SK13_067, a second allele-specific forward primer represented by SEQ ID NO: 950, and a reverse primer set represented by SEQ ID NO: 951, for SK13_071 A set of first allele-specific forward primers represented by SEQ ID NO: 952, second allele-specific forward primers represented by SEQ ID NO: 953 and reverse primers represented by SEQ ID NO: 954, the first set represented by SEQ ID NO: 955 for SK13_080 Allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 956 and the reverse primer set represented by SEQ ID NO: 957, the first allele-specific forward primer represented by SEQ ID NO: 958 for SK13_088, SEQ ID NO: The second allele-specific forward primer represented by SEQ ID NO: 959 and the reverse primer set represented by SEQ ID NO: 960, the first allele-specific forward primer represented by SEQ ID NO: 961 for SK13_094, the second allele represented by SEQ ID NO: 962 A set of specific forward primers and reverse primers represented by SEQ ID NO: 963, a first allele-specific forward primer represented by SEQ ID NO: 964 for SK13_103, a second allele-specific forward primer represented by SEQ ID NO: 965, and SEQ ID NO: 966. The reverse primer set represented by, the first allele-specific forward primer represented by SEQ ID NO: 967 for SK13_107, the second allele-specific forward primer represented by SEQ ID NO: 968, and the reverse primer set represented by SEQ ID NO: 969, SK13_113 The first allele-specific forward primer represented by SEQ ID NO: 970, the second allele-specific forward primer represented by SEQ ID NO: 971, and the reverse primer set represented by SEQ ID NO: 972, SEQ ID NO: 973 for SK13_123 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 974 and the reverse primer set represented by SEQ ID NO: 975, the first allele-specific forward primer represented by SEQ ID NO: 976 for SK13_127, The second allele-specific forward primer represented by SEQ ID NO: 977 and the reverse primer set represented by SEQ ID NO: 978, the first allele-specific forward primer represented by SEQ ID NO: 979 for SK13_131, the second allele-specific forward primer represented by SEQ ID NO: 980 Allele-specific forward primer and reverse primer set represented by SEQ ID NO: 981, first allele-specific forward primer represented by SEQ ID NO: 982 for SK13_138, second allele-specific forward primer represented by SEQ ID NO: 983 and SEQ ID NO: A reverse primer set represented by SEQ ID NO: 984, a first allele-specific forward primer represented by SEQ ID NO: 985 for SK13_150, a second allele-specific forward primer represented by SEQ ID NO: 986, and a reverse primer set represented by SEQ ID NO: 987 , the first allele-specific forward primer represented by SEQ ID NO: 988 for SK13_157, the second allele-specific forward primer represented by SEQ ID NO: 989 and the reverse primer set represented by SEQ ID NO: 990, SEQ ID NO: 991 for SK13_164 It includes a primer set selected from the group consisting of a first allele-specific forward primer represented by SEQ ID NO: 992, a second allele-specific forward primer represented by SEQ ID NO: 992, and a reverse primer set represented by SEQ ID NO: 993, specifically 19 It may include more than one primer set, but is not limited thereto.

The primer set corresponds to a primer set for amplifying a KASP marker for genetic mapping of sesame varieties.

In the present invention, "KASP (kompetitive allele specific PCR)" is one of PCR-based analysis methods, and is a homogenous and fluorescence-based genotyping technology. KASP is a technology based on fluorescence resonance energy transfer for allele-specific oligo extension and signal generation.

The KASP marker set of the present invention is a primer set for detecting the base type of a single nucleotide polymorphism (SNP) marker specifically differentiated in 46 varieties of sesame seeds.

The 46 varieties of sesame seeds are Gilmi, Kangbaek, Gangan, Kangheuk, Geonbaek, Geonheuk, Gopum, Geumok, Nambaek, Namsan, Naman, Nuri, Dasak, Daheuk, Dubeol, Mangeum, Manheuk, Miheuk, Milseong, Sangbaek, Suwon, Sunheuk, Annam, Ansan, Yangheuk, Osan, Yumi, Yuseong, Yunheuk, Jungmo 5002, Jungmo 5003, Jungmo 5005, Jingi, Jinbaek, Jinyul, Jinju, Chamhwang, Pyeongan, Pungnam, Poongsan, Pungan, Hansan, Handsome, Hwaheuk, Hwangbaek, Heukseon am.

In the primer set for KASP of the present invention, the characteristics of sesame varieties can be determined by combining two forward primers having partially different base sequences from a fluorescent sample to the same reverse primer.

In the present invention, "primer" refers to a single-stranded oligonucleotide sequence complementary to a nucleic acid strand to be replicated, and can serve as a starting point for synthesis of a primer extension product. The length and sequence of the primers should allow for the synthesis of extension products to begin. The specific length and sequence of the primer will depend on the complexity of the DNA or RNA target required, as well as the conditions of use of the primer, such as temperature and ionic strength.

In this specification, oligonucleotides used as primers may also include nucleotide analogs, such as phosphorothioates, alkylphosphorothioates, or peptide nucleic acids, or Intercalating agents may be included.

The KASP marker set of the present invention has a polymorphism information content (PIC) value of 0.4 among single nucleotide polymorphisms (SNPs) or insertion and deletion (InDel) found through genome resequencing of 13 domestic sesame varieties. Among approximately 260,000 abnormal genetic variants, 396 genetic variants with a distance of 500 to 800 Mb within the genome were selected to create a KASP marker assay. As a result of genotyping these KASP marker assays in 46 domestic sesame varieties, the final 331 KASP markers were stably PCR amplified, showing polymorphism between varieties. In order to construct a genetic linkage map using the 331 KASP markers, the physical locations of 13 chromosomes of sesame seeds were mapped.

The profiling information of 331 KASP marker primer sets on 46 domestic sesame varieties was classified and displayed by allele. When the KASP marker was analyzed with the FAM fluorescent marker, it was regarded as a type 1 allele and indicated as “A”, and when analyzed with the HEX fluorescent marker, it was regarded as a type 2 allele and indicated as “B”. In the case of an intermediate type having all alleles, it was indicated as hetero “H”, and the case where the genotype was not clear or PCR failed was left blank.

Heuristic algorithm-based PoweCore program (http://genebank.rda.go kr/powercore.do) was used to select the minimum marker.

Therefore, the primer set of the present invention includes 19 or more primer sets selected from the group consisting of 331 primer sets consisting of oligonucleotides of SEQ ID NO: 1 to SEQ ID NO: 993, and specifically, the KASP marker set for genetic mapping The first allele-specific forward primer represented by SEQ ID NO: 1 for SK01_007, the second allele-specific forward primer represented by SEQ ID NO: 2, and the reverse primer set represented by SEQ ID NO: 3, SEQ ID NO: 4 for SK01_012 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 5 and the reverse primer set represented by SEQ ID NO: 6, the first allele-specific forward primer represented by SEQ ID NO: 7 for SK01_018, sequence The second allele-specific forward primer represented by No. 8 and the reverse primer set represented by SEQ ID NO: 9, the first allele-specific forward primer represented by SEQ ID NO: 10 for SK01_025, the second allele represented by SEQ ID NO: 11 A set of specific forward primers and reverse primers represented by SEQ ID NO: 12, a first allele-specific forward primer represented by SEQ ID NO: 13 for SK01_030, a second allele-specific forward primer represented by SEQ ID NO: 14, and SEQ ID NO: 15 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 16 for SK01_038, a second allele-specific forward primer represented by SEQ ID NO: 17, and a reverse primer set represented by SEQ ID NO: 18, sequence for SK01_046 The first allele-specific forward primer represented by SEQ ID NO: 19, the second allele-specific forward primer represented by SEQ ID NO: 20, and the reverse primer set represented by SEQ ID NO: 21, the first allele represented by SEQ ID NO: 22 for SK01_054 A specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 23 and a reverse primer set represented by SEQ ID NO: 24, a first allele-specific forward primer represented by SEQ ID NO: 25 for SKJ01_070, represented by SEQ ID NO: 26 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 27, a first allele-specific forward primer represented by SEQ ID NO: 28 for SK01_084, a second allele-specific forward primer represented by SEQ ID NO: 29, and A reverse primer set represented by SEQ ID NO: 30, a first allele-specific forward primer represented by SEQ ID NO: 31 for SK01_093, a second allele-specific forward primer represented by SEQ ID NO: 32, and a reverse primer set represented by SEQ ID NO: 33 , the first allele-specific forward primer represented by SEQ ID NO: 34 for SK01_102, the second allele-specific forward primer represented by SEQ ID NO: 35 and the reverse primer set represented by SEQ ID NO: 36, SEQ ID NO: 37 for SK01_109 A set of first allele-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 38 and a reverse primer set represented by SEQ ID NO: 39, the first allele-specific forward primer represented by SEQ ID NO: 40 for SK01_113 , the second allele-specific forward primer represented by SEQ ID NO: 41 and the reverse primer set represented by SEQ ID NO: 42, the first allele-specific forward primer represented by SEQ ID NO: 43 for SK01_120, the second allele-specific forward primer represented by SEQ ID NO: 44 Allele-specific forward primer and reverse primer set represented by SEQ ID NO: 45, first allele-specific forward primer represented by SEQ ID NO: 46 for SK01_128, second allele-specific forward primer represented by SEQ ID NO: 47 and SEQ ID NO: 48 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 49 for SK01_133, a second allele-specific forward primer represented by SEQ ID NO: 50, and a reverse primer set represented by SEQ ID NO: 51, to SK01_139 A set of a first allele-specific forward primer represented by SEQ ID NO: 52, a second allele-specific forward primer represented by SEQ ID NO: 53, and a reverse primer set represented by SEQ ID NO: 54, a first set represented by SEQ ID NO: 55 for SK01_144 An allele-specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 56 and a set of reverse primers represented by SEQ ID NO: 57, a first allele-specific forward primer represented by SEQ ID NO: 58 for SK01_152, SEQ ID NO: 59 A second allele-specific forward primer represented by and a reverse primer set represented by SEQ ID NO: 60, a first allele-specific forward primer represented by SEQ ID NO: 61 for SK01_160, and a second allele-specific forward primer represented by SEQ ID NO: 62 A set of primers and reverse primers represented by SEQ ID NO: 63, a first allele-specific forward primer represented by SEQ ID NO: 64 for SK01_174, a second allele-specific forward primer represented by SEQ ID NO: 65, and a reverse primer represented by SEQ ID NO: 66 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 67 for SK01_181, a second allele-specific forward primer represented by SEQ ID NO: 68, and a reverse primer set represented by SEQ ID NO: 69, SEQ ID NO: 70 for SK01_187 The first allele-specific forward primer represented by SEQ ID NO: 71, the second allele-specific forward primer represented by SEQ ID NO: 72, and the reverse primer set represented by SEQ ID NO: 72, the first allele-specific forward primer represented by SEQ ID NO: 73 for SK01_194 Primers, the second allele-specific forward primer represented by SEQ ID NO: 74 and the reverse primer set represented by SEQ ID NO: 75, the first allele-specific forward primer represented by SEQ ID NO: 76 for SK01_201, the first allele-specific forward primer represented by SEQ ID NO: 77 2 Allele-specific forward primer and reverse primer set represented by SEQ ID NO: 78, the first allele-specific forward primer represented by SEQ ID NO: 79 for SK02_007, the second allele-specific forward primer represented by SEQ ID NO: 80 and SEQ ID NO: A reverse primer set represented by SEQ ID NO: 81, a first allele-specific forward primer represented by SEQ ID NO: 82 for SK02_013, a second allele-specific forward primer represented by SEQ ID NO: 83, and a reverse primer set represented by SEQ ID NO: 84, SK02_022 The first allele-specific forward primer represented by SEQ ID NO: 85, the second allele-specific forward primer represented by SEQ ID NO: 86, and the reverse primer set represented by SEQ ID NO: 87, SEQ ID NO: 88 for SK02_027 The first allele specific forward primer, the second allele specific forward primer represented by SEQ ID NO: 89 and the reverse primer set represented by SEQ ID NO: 90, the first allele specific forward primer represented by SEQ ID NO: 91 for SK02-036 Primers, the second allele-specific forward primer represented by SEQ ID NO: 92 and the reverse primer set represented by SEQ ID NO: 93, the first allele-specific forward primer represented by SEQ ID NO: 94 for SK02_041, SEQ ID NO: 95 A second allele-specific forward primer and a set of reverse primers represented by SEQ ID NO: 96, a first allele-specific forward primer represented by SEQ ID NO: 97 for SK02_050, a second allele-specific forward primer represented by SEQ ID NO: 98, and A reverse primer set represented by SEQ ID NO: 99, a first allele-specific forward primer represented by SEQ ID NO: 100 for SK02_059, a second allele-specific forward primer represented by SEQ ID NO: 101, and a reverse primer represented by SEQ ID NO: 102 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 103 for SK02_070, a second allele-specific forward primer represented by SEQ ID NO: 104, and a reverse primer set represented by SEQ ID NO: 105, SEQ ID NO: SK02_076 The first allele-specific forward primer represented by SEQ ID NO: 106, the second allele-specific forward primer represented by SEQ ID NO: 107, and the reverse primer set represented by SEQ ID NO: 108, the first allele represented by SEQ ID NO: 109 for SK02_081 A specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 110 and a set of reverse primers represented by SEQ ID NO: 111, a first allele-specific forward primer represented by SEQ ID NO: 112 for SK02_087, SEQ ID NO: 113 The second allele-specific forward primer and the reverse primer set represented by SEQ ID NO: 114, the first allele-specific forward primer represented by SEQ ID NO: 115 for SK02_089, and the second allele-specific forward primer represented by SEQ ID NO: 116 Primers and a reverse primer set represented by SEQ ID NO: 117, a first allele-specific forward primer represented by SEQ ID NO: 118 for SK02_097, a second allele-specific forward primer represented by SEQ ID NO: 119, and SEQ ID NO: 120 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 121 for SK02_103, a second allele-specific forward primer represented by SEQ ID NO: 122, and a reverse primer set represented by SEQ ID NO: 123, for SK02_110 A set of first allele-specific forward primers represented by SEQ ID NO: 124, second allele-specific forward primers represented by SEQ ID NO: 125 and reverse primers represented by SEQ ID NO: 126, the first set represented by SEQ ID NO: 127 for SK02_117 Allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 128 and the reverse primer set represented by SEQ ID NO: 129, the first allele-specific forward primer represented by SEQ ID NO: 130 for SK02_121, SEQ ID NO: The second allele-specific forward primer represented by SEQ ID NO: 131 and the reverse primer set represented by SEQ ID NO: 132, the first allele-specific forward primer represented by SEQ ID NO: 133 for SK02_133, the second allele represented by SEQ ID NO: 134 A specific forward primer and a set of reverse primers represented by SEQ ID NO: 135, a first allele-specific forward primer represented by SEQ ID NO: 136 for SK02_140, a second allele-specific forward primer represented by SEQ ID NO: 137, and SEQ ID NO: 138 The reverse primer set represented by, the first allele-specific forward primer represented by SEQ ID NO: 139 for SK02_151, the second allele-specific forward primer represented by SEQ ID NO: 140, and the reverse primer set represented by SEQ ID NO: 141, SK02_160 The first allele-specific forward primer represented by SEQ ID NO: 142, the second allele-specific forward primer represented by SEQ ID NO: 143, and the reverse primer set represented by SEQ ID NO: 144, SEQ ID NO: 145 for SK02_167 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 146 and the reverse primer set represented by SEQ ID NO: 147, the first allele-specific forward primer represented by SEQ ID NO: 148 for SK02_179, The second allele-specific forward primer represented by SEQ ID NO: 149 and the reverse primer set represented by SEQ ID NO: 150, the first allele-specific forward primer represented by SEQ ID NO: 151 for SK03_003, and the second allele-specific forward primer represented by SEQ ID NO: 152 Allele-specific forward primer and reverse primer set represented by SEQ ID NO: 153, the first allele-specific forward primer represented by SEQ ID NO: 154 for SK03_012, the second allele-specific forward primer represented by SEQ ID NO: 155, and SEQ ID NO: A reverse primer set represented by SEQ ID NO: 156, a first allele-specific forward primer represented by SEQ ID NO: 157 for SK03_020, a second allele-specific forward primer represented by SEQ ID NO: 158, and a reverse primer set represented by SEQ ID NO: 159 , the first allele-specific forward primer represented by SEQ ID NO: 160 for SK03_029, the second allele-specific forward primer represented by SEQ ID NO: 161 and the reverse primer set represented by SEQ ID NO: 162, SEQ ID NO: 163 for SK03_030. The first allele-specific forward primer represented by SEQ ID NO: 164, the second allele-specific forward primer represented by SEQ ID NO: 165, and the reverse primer set represented by SEQ ID NO: 165, the first allele-specific forward primer represented by SEQ ID NO: 166 for SK03_038 Primers, the second allele-specific forward primer represented by SEQ ID NO: 167 and the reverse primer set represented by SEQ ID NO: 168, the first allele-specific forward primer represented by SEQ ID NO: 169 for SK03_044, SEQ ID NO: 170 A second allele-specific forward primer and a set of reverse primers represented by SEQ ID NO: 171, a first allele-specific forward primer represented by SEQ ID NO: 172 for SK03_050, a second allele-specific forward primer represented by SEQ ID NO: 173, and A reverse primer set represented by SEQ ID NO: 174, a first allele-specific forward primer represented by SEQ ID NO: 175 for SK03_055, a second allele-specific forward primer represented by SEQ ID NO: 176, and a reverse primer represented by SEQ ID NO: 177 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 178 for SK03_063, a second allele-specific forward primer represented by SEQ ID NO: 179, and a reverse primer set represented by SEQ ID NO: 180, SEQ ID NO: SK03_070 The first allele-specific forward primer represented by SEQ ID NO: 181, the second allele-specific forward primer represented by SEQ ID NO: 182, and the reverse primer set represented by SEQ ID NO: 183, the first allele represented by SEQ ID NO: 184 for SK03_079 A specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 185 and a set of reverse primers represented by SEQ ID NO: 186, a first allele-specific forward primer represented by SEQ ID NO: 187 for SK03_094, SEQ ID NO: 188 The second allele-specific forward primer and the reverse primer set represented by SEQ ID NO: 189, the first allele-specific forward primer represented by SEQ ID NO: 190 for SK03_098, and the second allele-specific forward primer represented by SEQ ID NO: 191 Primers and a set of reverse primers represented by SEQ ID NO: 192, a first allele-specific forward primer represented by SEQ ID NO: 193 for SK03_102, a second allele-specific forward primer represented by SEQ ID NO: 194, and SEQ ID NO: 195 The reverse primer set, the first allele-specific forward primer represented by SEQ ID NO: 196 for SK03_108, the second allele-specific forward primer represented by SEQ ID NO: 197, and the reverse primer set represented by SEQ ID NO: 198, for SK03_116 A set of first allele-specific forward primers represented by SEQ ID NO: 199, second allele-specific forward primers represented by SEQ ID NO: 200 and reverse primers represented by SEQ ID NO: 201, the first set represented by SEQ ID NO: 202 for SK03_120 Allele-specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 203 and a set of reverse primers represented by SEQ ID NO: 204, a first allele-specific forward primer represented by SEQ ID NO: 205 for SK03_126, SEQ ID NO: The second allele-specific forward primer represented by SEQ ID NO: 206 and the reverse primer set represented by SEQ ID NO: 207, the first allele-specific forward primer represented by SEQ ID NO: 208 for SK03_141, the second allele represented by SEQ ID NO: 209 A specific forward primer and a set of reverse primers represented by SEQ ID NO: 210, a first allele-specific forward primer represented by SEQ ID NO: 211 for SK03_147, a second allele-specific forward primer represented by SEQ ID NO: 212, and SEQ ID NO: 213 The reverse primer set represented by, the first allele-specific forward primer represented by SEQ ID NO: 214 for SK03_151, the second allele-specific forward primer represented by SEQ ID NO: 215, and the reverse primer set represented by SEQ ID NO: 216, SK03_158 The first allele-specific forward primer represented by SEQ ID NO: 217, the second allele-specific forward primer represented by SEQ ID NO: 218, and the reverse primer set represented by SEQ ID NO: 219, SEQ ID NO: 220 for SK03_166 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 221 and the reverse primer set represented by SEQ ID NO: 222, the first allele-specific forward primer represented by SEQ ID NO: 223 for SK03_173, The second allele-specific forward primer represented by SEQ ID NO: 224 and the reverse primer set represented by SEQ ID NO: 225, the first allele-specific forward primer represented by SEQ ID NO: 226 for SK03_180, the second allele-specific forward primer represented by SEQ ID NO: 227 Allele-specific forward primer and reverse primer set represented by SEQ ID NO: 228, the first allele-specific forward primer represented by SEQ ID NO: 229 for SK03_191, the second allele-specific forward primer represented by SEQ ID NO: 230, and SEQ ID NO: A reverse primer set represented by SEQ ID NO: 231, a first allele-specific forward primer represented by SEQ ID NO: 232 for SK03_203, a second allele-specific forward primer represented by SEQ ID NO: 233, and a reverse primer set represented by SEQ ID NO: 234 , the first allele-specific forward primer represented by SEQ ID NO: 235 for SK03_219, the second allele-specific forward primer represented by SEQ ID NO: 236 and the reverse primer set represented by SEQ ID NO: 237, SEQ ID NO: 238 for SK03_226 The first allele-specific forward primer represented by SEQ ID NO: 239, the second allele-specific forward primer represented by SEQ ID NO: 240, and the set of reverse primers represented by SEQ ID NO: 240, the first allele-specific forward primer represented by SEQ ID NO: 241 for SK03_237 Primers, the second allele-specific forward primer represented by SEQ ID NO: 242 and the reverse primer set represented by SEQ ID NO: 243, the first allele-specific forward primer represented by SEQ ID NO: 244 for SK03_245, SEQ ID NO: 245 A second allele-specific forward primer and a set of reverse primers represented by SEQ ID NO: 246, a first allele-specific forward primer represented by SEQ ID NO: 247 for SK03_252, a second allele-specific forward primer represented by SEQ ID NO: 248, and A reverse primer set represented by SEQ ID NO: 249, a first allele-specific forward primer represented by SEQ ID NO: 250 for SK03_258, a second allele-specific forward primer represented by SEQ ID NO: 251, and a reverse primer represented by SEQ ID NO: 252 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 253 for SK04_008, a second allele-specific forward primer represented by SEQ ID NO: 254, and a reverse primer set represented by SEQ ID NO: 255, SEQ ID NO: 255 The first allele-specific forward primer represented by SEQ ID NO: 256, the second allele-specific forward primer represented by SEQ ID NO: 257, and the reverse primer set represented by SEQ ID NO: 258, the first allele represented by SEQ ID NO: 259 for SK04_023 A specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 260 and a set of reverse primers represented by SEQ ID NO: 261, a first allele-specific forward primer represented by SEQ ID NO: 262 for SK04_029, SEQ ID NO: 263 The second allele-specific forward primer and the reverse primer set represented by SEQ ID NO: 264, the first allele-specific forward primer represented by SEQ ID NO: 265 for SK04_045, and the second allele-specific forward primer represented by SEQ ID NO: 266 Primers and a set of reverse primers represented by SEQ ID NO: 267, a first allele-specific forward primer represented by SEQ ID NO: 268 for SK04_051, a second allele-specific forward primer represented by SEQ ID NO: 269, and SEQ ID NO: 270 The reverse primer set, the first allele-specific forward primer represented by SEQ ID NO: 271 for SK04_060, the second allele-specific forward primer represented by SEQ ID NO: 272, and the reverse primer set represented by SEQ ID NO: 273, for SK04_069 A set of first allele-specific forward primers represented by SEQ ID NO: 274, second allele-specific forward primers represented by SEQ ID NO: 275 and reverse primers represented by SEQ ID NO: 276, the first set represented by SEQ ID NO: 277 for SK04_080 Allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 278 and the set of reverse primers represented by SEQ ID NO: 279, the first allele-specific forward primer represented by SEQ ID NO: 280 for SK04_090, SEQ ID NO: The second allele-specific forward primer represented by SEQ ID NO: 281 and the reverse primer set represented by SEQ ID NO: 282, the first allele-specific forward primer represented by SEQ ID NO: 283 for SK04_098, the second allele represented by SEQ ID NO: 284 A specific forward primer and a set of reverse primers represented by SEQ ID NO: 285, a first allele-specific forward primer represented by SEQ ID NO: 286 for SK04_107, a second allele-specific forward primer represented by SEQ ID NO: 287, and SEQ ID NO: 288 The reverse primer set represented by, the first allele-specific forward primer represented by SEQ ID NO: 289 for SK04_114, the second allele-specific forward primer represented by SEQ ID NO: 290, and the reverse primer set represented by SEQ ID NO: 291, SK04_122 The first allele-specific forward primer represented by SEQ ID NO: 292, the second allele-specific forward primer represented by SEQ ID NO: 293, and the reverse primer set represented by SEQ ID NO: 294, SEQ ID NO: 295 for SK04_129 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 296 and the reverse primer set represented by SEQ ID NO: 297, the first allele-specific forward primer represented by SEQ ID NO: 298 for SK04_138, The second allele-specific forward primer represented by SEQ ID NO: 299 and the reverse primer set represented by SEQ ID NO: 300, the first allele-specific forward primer represented by SEQ ID NO: 301 for SK04_143, and the second allele-specific forward primer represented by SEQ ID NO: 302 Allele-specific forward primer and reverse primer set represented by SEQ ID NO: 303, first allele-specific forward primer represented by SEQ ID NO: 304 for SK04_149, second allele-specific forward primer represented by SEQ ID NO: 305 and SEQ ID NO: A reverse primer set represented by SEQ ID NO: 306, a first allele-specific forward primer represented by SEQ ID NO: 307 for SK04_158, a second allele-specific forward primer represented by SEQ ID NO: 308, and a reverse primer set represented by SEQ ID NO: 309 , the first allele-specific forward primer represented by SEQ ID NO: 310 for SK04_164, the second allele-specific forward primer represented by SEQ ID NO: 311 and the reverse primer set represented by SEQ ID NO: 312, SEQ ID NO: 313 for SK04_174 The first allele-specific forward primer represented by SEQ ID NO: 314, the second allele-specific forward primer represented by SEQ ID NO: 315, and the set of reverse primers represented by SEQ ID NO: 315, the first allele-specific forward primer represented by SEQ ID NO: 316 for SK04_180 Primers, the second allele-specific forward primer represented by SEQ ID NO: 317 and the reverse primer set represented by SEQ ID NO: 318, the first allele-specific forward primer represented by SEQ ID NO: 319 for SK04_183, SEQ ID NO: 320 A second allele-specific forward primer and a set of reverse primers represented by SEQ ID NO: 321, a first allele-specific forward primer represented by SEQ ID NO: 322 for SK04_189, a second allele-specific forward primer represented by SEQ ID NO: 323, and A reverse primer set represented by SEQ ID NO: 324, a first allele-specific forward primer represented by SEQ ID NO: 325 for SK04_195, a second allele-specific forward primer represented by SEQ ID NO: 326, and a reverse primer represented by SEQ ID NO: 327 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 328 for SK04_200, a second allele-specific forward primer represented by SEQ ID NO: 329, and a reverse primer set represented by SEQ ID NO: 330, SEQ ID NO: The first allele-specific forward primer represented by SEQ ID NO: 331, the second allele-specific forward primer represented by SEQ ID NO: 332, and the reverse primer set represented by SEQ ID NO: 333, the first allele represented by SEQ ID NO: 334 for SK05_003 A specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 335 and a set of reverse primers represented by SEQ ID NO: 336, a first allele-specific forward primer represented by SEQ ID NO: 337 for SK05_010, SEQ ID NO: 338 The second allele-specific forward primer and the reverse primer set represented by SEQ ID NO: 339, the first allele-specific forward primer represented by SEQ ID NO: 340 for SK05_015, and the second allele-specific forward primer represented by SEQ ID NO: 341 Primers and a set of reverse primers represented by SEQ ID NO: 342, a first allele-specific forward primer represented by SEQ ID NO: 343 for SK05_017, a second allele-specific forward primer represented by SEQ ID NO: 344, and SEQ ID NO: 345 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 346 for SK05_032, a second allele-specific forward primer represented by SEQ ID NO: 347, and a reverse primer set represented by SEQ ID NO: 348, for SK05_036 A set of first allele-specific forward primers represented by SEQ ID NO: 349, second allele-specific forward primers represented by SEQ ID NO: 350 and reverse primers represented by SEQ ID NO: 351, the first set represented by SEQ ID NO: 352 for SK05_039 Allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 353 and the set of reverse primers represented by SEQ ID NO: 354, the first allele-specific forward primer represented by SEQ ID NO: 355 for SK05_059, SEQ ID NO: The second allele-specific forward primer represented by SEQ ID NO: 356 and the reverse primer set represented by SEQ ID NO: 357, the first allele-specific forward primer represented by SEQ ID NO: 358 for SK05_068, the second allele represented by SEQ ID NO: 359 A set of specific forward primers and reverse primers represented by SEQ ID NO: 360, a first allele-specific forward primer represented by SEQ ID NO: 361 for SK05_075, a second allele-specific forward primer represented by SEQ ID NO: 362, and SEQ ID NO: 363. The reverse primer set represented by, the first allele-specific forward primer represented by SEQ ID NO: 364 for SK05_087, the second allele-specific forward primer represented by SEQ ID NO: 365, and the reverse primer set represented by SEQ ID NO: 366, SK05_092 The first allele-specific forward primer represented by SEQ ID NO: 367, the second allele-specific forward primer represented by SEQ ID NO: 368, and the reverse primer set represented by SEQ ID NO: 369, SEQ ID NO: 370 for SK05_100 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 371 and the reverse primer set represented by SEQ ID NO: 372, the first allele-specific forward primer represented by SEQ ID NO: 373 for SK05_108, The second allele-specific forward primer represented by SEQ ID NO: 374 and the reverse primer set represented by SEQ ID NO: 375, the first allele-specific forward primer represented by SEQ ID NO: 374 for SK05_112, the second allele-specific forward primer represented by SEQ ID NO: 375 Allele-specific forward primer and reverse primer set represented by SEQ ID NO: 376, first allele-specific forward primer represented by SEQ ID NO: 379 for SK05_115, second allele-specific forward primer represented by SEQ ID NO: 380 and SEQ ID NO: A reverse primer set represented by 381, a first allele-specific forward primer represented by SEQ ID NO: 382 for SK05_122, a second allele-specific forward primer represented by SEQ ID NO: 383, and a reverse primer set represented by SEQ ID NO: 384 , the first allele-specific forward primer represented by SEQ ID NO: 385 for SK05_132, the second allele-specific forward primer represented by SEQ ID NO: 386 and the reverse primer set represented by SEQ ID NO: 387, SEQ ID NO: 388 for SK05_137 The first allele-specific forward primer represented by SEQ ID NO: 389, the second allele-specific forward primer represented by SEQ ID NO: 390, and the set of reverse primers represented by SEQ ID NO: 390, the first allele-specific forward primer represented by SEQ ID NO: 391 for SK05_148 Primers, the second allele-specific forward primer represented by SEQ ID NO: 392 and the reverse primer set represented by SEQ ID NO: 393, the first allele-specific forward primer represented by SEQ ID NO: 394 for SK05_155, SEQ ID NO: 395 A second allele-specific forward primer and a set of reverse primers represented by SEQ ID NO: 396, a first allele-specific forward primer represented by SEQ ID NO: 397 for SK05_162, a second allele-specific forward primer represented by SEQ ID NO: 398, and A reverse primer set represented by SEQ ID NO: 399, a first allele-specific forward primer represented by SEQ ID NO: 400 for SK06_006, a second allele-specific forward primer represented by SEQ ID NO: 401, and a reverse primer represented by SEQ ID NO: 402 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 403 for SK06_013, a second allele-specific forward primer represented by SEQ ID NO: 404, and a reverse primer set represented by SEQ ID NO: 405, SEQ ID NO: SK06_022 The first allele-specific forward primer represented by SEQ ID NO: 406, the second allele-specific forward primer represented by SEQ ID NO: 407, and the reverse primer set represented by SEQ ID NO: 408, the first allele represented by SEQ ID NO: 409 for SK06_029 A specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 410 and a set of reverse primers represented by SEQ ID NO: 411, a first allele-specific forward primer represented by SEQ ID NO: 412 for SK06_035, SEQ ID NO: 413 The second allele-specific forward primer and the reverse primer set represented by SEQ ID NO: 414, the first allele-specific forward primer represented by SEQ ID NO: 415 for SK06_042, and the second allele-specific forward primer represented by SEQ ID NO: 416 Primers and a set of reverse primers represented by SEQ ID NO: 417, a first allele-specific forward primer represented by SEQ ID NO: 418 for SK06_048, a second allele-specific forward primer represented by SEQ ID NO: 419, and SEQ ID NO: 420 The reverse primer set, the first allele-specific forward primer represented by SEQ ID NO: 421 for SK06_056, the second allele-specific forward primer represented by SEQ ID NO: 422, and the reverse primer set represented by SEQ ID NO: 423, for SK06_063 A set of first allele-specific forward primers represented by SEQ ID NO: 424, second allele-specific forward primers represented by SEQ ID NO: 425, and reverse primers represented by SEQ ID NO: 426, the first set represented by SEQ ID NO: 427 for SK06_067 Allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 428 and the reverse primer set represented by SEQ ID NO: 429, the first allele-specific forward primer represented by SEQ ID NO: 430 for SK06_075, SEQ ID NO: The second allele-specific forward primer represented by 431 and the reverse primer set represented by SEQ ID NO: 432, the first allele-specific forward primer represented by SEQ ID NO: 433 for SK06_081, the second allele represented by SEQ ID NO: 434 A set of specific forward primers and reverse primers represented by SEQ ID NO: 435, a first allele-specific forward primer represented by SEQ ID NO: 436 for SK06_098, a second allele-specific forward primer represented by SEQ ID NO: 437, and SEQ ID NO: 438. The reverse primer set represented by, the first allele-specific forward primer represented by SEQ ID NO: 439 for SK06_105, the second allele-specific forward primer represented by SEQ ID NO: 440, and the reverse primer set represented by SEQ ID NO: 441, SK06_113 The first allele-specific forward primer represented by SEQ ID NO: 442, the second allele-specific forward primer represented by SEQ ID NO: 443, and the reverse primer set represented by SEQ ID NO: 444, SEQ ID NO: 445 for SK06_121 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 446 and the reverse primer set represented by SEQ ID NO: 447, the first allele-specific forward primer represented by SEQ ID NO: 448 for SK06_129, The second allele-specific forward primer represented by SEQ ID NO: 449 and the reverse primer set represented by SEQ ID NO: 450, the first allele-specific forward primer represented by SEQ ID NO: 451 for SK06_136, the second allele-specific forward primer represented by SEQ ID NO: 452 An allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 453, a first allele-specific forward primer represented by SEQ ID NO: 454 for SK06_139, a second allele-specific forward primer represented by SEQ ID NO: 455, and SEQ ID NO: A reverse primer set represented by SEQ ID NO: 456, a first allele-specific forward primer represented by SEQ ID NO: 457 for SK06_145, a second allele-specific forward primer represented by SEQ ID NO: 458, and a reverse primer set represented by SEQ ID NO: 459 , the first allele-specific forward primer represented by SEQ ID NO: 460 for SK06_152, the second allele-specific forward primer represented by SEQ ID NO: 461 and the reverse primer set represented by SEQ ID NO: 462, SEQ ID NO: 463 for SK06_157 The first allele-specific forward primer represented by SEQ ID NO: 464, the second allele-specific forward primer represented by SEQ ID NO: 465, and the set of reverse primers represented by SEQ ID NO: 465, the first allele-specific forward primer represented by SEQ ID NO: 466 for SK06_162 Primers, the second allele-specific forward primer represented by SEQ ID NO: 467 and the reverse primer set represented by SEQ ID NO: 468, the first allele-specific forward primer represented by SEQ ID NO: 469 for SK06_170, SEQ ID NO: 470 A second allele-specific forward primer and a set of reverse primers represented by SEQ ID NO: 471, a first allele-specific forward primer represented by SEQ ID NO: 472 for SK06_177, a second allele-specific forward primer represented by SEQ ID NO: 473, and A reverse primer set represented by SEQ ID NO: 474, a first allele-specific forward primer represented by SEQ ID NO: 475 for SK06_183, a second allele-specific forward primer represented by SEQ ID NO: 476, and a reverse primer represented by SEQ ID NO: 477 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 478 for SK06_190, a second allele-specific forward primer represented by SEQ ID NO: 479, and a reverse primer set represented by SEQ ID NO: 480, SEQ ID NO: The first allele-specific forward primer represented by SEQ ID NO: 481, the second allele-specific forward primer represented by SEQ ID NO: 482, and the reverse primer set represented by SEQ ID NO: 483, the first allele represented by SEQ ID NO: 484 for SK06_198 A specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 485 and a set of reverse primers represented by SEQ ID NO: 486, a first allele-specific forward primer represented by SEQ ID NO: 487 for SK06_205, SEQ ID NO: 488 The second allele-specific forward primer and the reverse primer set represented by SEQ ID NO: 489, the first allele-specific forward primer represented by SEQ ID NO: 490 for SK06_212, and the second allele-specific forward primer represented by SEQ ID NO: 491 Primers and a set of reverse primers represented by SEQ ID NO: 492, a first allele-specific forward primer represented by SEQ ID NO: 493 for SK06_222, a second allele-specific forward primer represented by SEQ ID NO: 494, and SEQ ID NO: 495 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 496 for SK06_235, a second allele-specific forward primer represented by SEQ ID NO: 497, and a reverse primer set represented by SEQ ID NO: 498, for SK06_240 A set of first allele-specific forward primers represented by SEQ ID NO: 499, second allele-specific forward primers represented by SEQ ID NO: 500 and reverse primers represented by SEQ ID NO: 501, the first set represented by SEQ ID NO: 502 for SK06_249 Allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 503 and the reverse primer set represented by SEQ ID NO: 504, the first allele-specific forward primer represented by SEQ ID NO: 505 for SK06_256, SEQ ID NO: The second allele-specific forward primer represented by SEQ ID NO: 506 and the reverse primer set represented by SEQ ID NO: 507, the first allele-specific forward primer represented by SEQ ID NO: 508 for SK07_006, the second allele represented by SEQ ID NO: 509 A specific forward primer and a set of reverse primers represented by SEQ ID NO: 510, a first allele-specific forward primer represented by SEQ ID NO: 511 for SK07_022, a second allele-specific forward primer represented by SEQ ID NO: 512, and SEQ ID NO: 513 The reverse primer set represented by, the first allele-specific forward primer represented by SEQ ID NO: 514 for SK07_043, the second allele-specific forward primer represented by SEQ ID NO: 515, and the reverse primer set represented by SEQ ID NO: 516, SK07_047 The first allele-specific forward primer represented by SEQ ID NO: 517, the second allele-specific forward primer represented by SEQ ID NO: 518, and the reverse primer set represented by SEQ ID NO: 519, SEQ ID NO: 520 for SK07_055 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 521 and the reverse primer set represented by SEQ ID NO: 522, the first allele-specific forward primer represented by SEQ ID NO: 523 for SK07_064, The second allele-specific forward primer represented by SEQ ID NO: 524 and the reverse primer set represented by SEQ ID NO: 525, the first allele-specific forward primer represented by SEQ ID NO: 526 for SK07_073, the second allele-specific forward primer represented by SEQ ID NO: 527 An allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 528, a first allele-specific forward primer represented by SEQ ID NO: 529 for SK07_079, a second allele-specific forward primer represented by SEQ ID NO: 530, and SEQ ID NO: A reverse primer set represented by 531, a first allele-specific forward primer represented by SEQ ID NO: 532 for SK07_083, a second allele-specific forward primer represented by SEQ ID NO: 533, and a reverse primer set represented by SEQ ID NO: 534 , the first allele-specific forward primer represented by SEQ ID NO: 535 for SK07_094, the second allele-specific forward primer represented by SEQ ID NO: 536 and the reverse primer set represented by SEQ ID NO: 537, SEQ ID NO: 538 for SK07_100. The first allele-specific forward primer represented by SEQ ID NO: 539, the second allele-specific forward primer represented by SEQ ID NO: 540, and the reverse primer set represented by SEQ ID NO: 541 for SK07_106. Primers, the second allele-specific forward primer represented by SEQ ID NO: 542 and the reverse primer set represented by SEQ ID NO: 543, the first allele-specific forward primer represented by SEQ ID NO: 544 for SK07_114, the first allele-specific forward primer represented by SEQ ID NO: 545 2 allele-specific forward primers and reverse primer set represented by SEQ ID NO: 546, the first allele-specific forward primer represented by SEQ ID NO: 547 for SK07_126, the second allele-specific forward primer represented by SEQ ID NO: 548 and sequence Reverse primer set represented by No. 549, the first allele-specific forward primer represented by SEQ ID NO: 550 for SK07_133, the second allele-specific forward primer represented by SEQ ID NO: 551, and the reverse primer represented by SEQ ID NO: 552 Set, a first allele specific forward primer represented by SEQ ID NO: 553 to SK07_130, a second allele specific forward primer represented by SEQ ID NO: 554 and a reverse primer set represented by SEQ ID NO: 555, SEQ ID NO: 556 to SK07_145 The first allele-specific forward primer represented by SEQ ID NO: 557, the second allele-specific forward primer represented by SEQ ID NO: 558, and the reverse primer set represented by SEQ ID NO: 558, the first allele-specific represented by SEQ ID NO: 559 for SK07_151 Forward primer, a second allele-specific forward primer represented by SEQ ID NO: 560 and a set of reverse primers represented by SEQ ID NO: 561, a first allele-specific forward primer represented by SEQ ID NO: 562 for SK07_164, SEQ ID NO: 563 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 564, a first allele-specific forward primer represented by SEQ ID NO: 565 for SK07_166, and a second allele-specific forward primer represented by SEQ ID NO: 566 and a set of reverse primers represented by SEQ ID NO: 567, a first allele-specific forward primer represented by SEQ ID NO: 568 for SK08_001, a second allele-specific forward primer represented by SEQ ID NO: 569, and a reverse sequence represented by SEQ ID NO: 570. The anti-primer set, the first allele-specific forward primer represented by SEQ ID NO: 571 for SK08_008, the second allele-specific forward primer represented by SEQ ID NO: 572, and the reverse primer set represented by SEQ ID NO: 573, the sequence for SK08_012 The first allele-specific forward primer represented by SEQ ID NO: 574, the second allele-specific forward primer represented by SEQ ID NO: 575, and the reverse primer set represented by SEQ ID NO: 576, the first allele represented by SEQ ID NO: 577 for SK08_020 Trait specific forward primer, second allele specific forward primer represented by SEQ ID NO: 578 and reverse primer set represented by SEQ ID NO: 579, first allele specific forward primer represented by SEQ ID NO: 580 for SK08_032, SEQ ID NO: 581 A second allele-specific forward primer represented by and a reverse primer set represented by SEQ ID NO: 582, a first allele-specific forward primer represented by SEQ ID NO: 583 for SK08_039, and a second allele-specific represented by SEQ ID NO: 584 A forward primer and a reverse primer set represented by SEQ ID NO: 585, a first allele-specific forward primer represented by SEQ ID NO: 586 for SK08_046, a second allele-specific forward primer represented by SEQ ID NO: 587, and SEQ ID NO: 588 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 589 for SK08_052, a second allele-specific forward primer represented by SEQ ID NO: 590, and a reverse primer set represented by SEQ ID NO: 591, SK08_066 The first allele-specific forward primer represented by SEQ ID NO: 592, the second allele-specific forward primer represented by SEQ ID NO: 593, and the reverse primer set represented by SEQ ID NO: 594, the first allele-specific forward primer represented by SEQ ID NO: 595 for SK08_072 1 allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 596 and the reverse primer set represented by SEQ ID NO: 597, the first allele-specific forward primer represented by SEQ ID NO: 598 for SK08_125, sequence The second allele-specific forward primer represented by No. 599 and the reverse primer set represented by SEQ ID NO: 600, the first allele-specific forward primer represented by SEQ ID NO: 601 for SK08_120, the second allele represented by SEQ ID NO: 602 A trait-specific forward primer and a set of reverse primers represented by SEQ ID NO: 603, a first allele-specific forward primer represented by SEQ ID NO: 604 for SK08_137, a second allele-specific forward primer represented by SEQ ID NO: 605, and SEQ ID NO: 606 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 607 for SK08_142, a second allele-specific forward primer represented by SEQ ID NO: 608, and a reverse primer set represented by SEQ ID NO: 609, A set of a first allele-specific forward primer represented by SEQ ID NO: 610 for SK08_148, a second allele-specific forward primer represented by SEQ ID NO: 611 and a reverse primer set represented by SEQ ID NO: 612, SEQ ID NO: 613 for SK08_156 A set of first allele-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 614 and a reverse primer set represented by SEQ ID NO: 615, the first allele-specific forward primer represented by SEQ ID NO: 616 for SK08_164 , the second allele-specific forward primer represented by SEQ ID NO: 617 and the reverse primer set represented by SEQ ID NO: 618, the first allele-specific forward primer represented by SEQ ID NO: 619 for SK08_172, the first allele-specific forward primer represented by SEQ ID NO: 620 2 allele-specific forward primers and reverse primer set represented by SEQ ID NO: 621, the first allele-specific forward primer represented by SEQ ID NO: 622 for SK08_179, the second allele-specific forward primer represented by SEQ ID NO: 623 and sequence Reverse primer set represented by No. 624, the first allele-specific forward primer represented by SEQ ID NO: 625 for SK08_187, the second allele-specific forward primer represented by SEQ ID NO: 626, and the reverse primer represented by SEQ ID NO: 627 Set, first allele specific forward primer represented by SEQ ID NO: 628 for SK08_197, second allele specific forward primer represented by SEQ ID NO: 629 and reverse primer set represented by SEQ ID NO: 630, SEQ ID NO: 631 for SK08_203 The first allele-specific forward primer represented by SEQ ID NO: 632, the second allele-specific forward primer represented by SEQ ID NO: 633, and the reverse primer set represented by SEQ ID NO: 633, the first allele-specific represented by SEQ ID NO: 634 for SK08_209 Forward primer, a second allele-specific forward primer represented by SEQ ID NO: 635 and a set of reverse primers represented by SEQ ID NO: 636, a first allele-specific forward primer represented by SEQ ID NO: 637 for SK08_216, SEQ ID NO: 638 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 639, a first allele-specific forward primer represented by SEQ ID NO: 640 for SK08_219, and a second allele-specific forward primer represented by SEQ ID NO: 641 and a reverse primer set represented by SEQ ID NO: 642, a first allele-specific forward primer represented by SEQ ID NO: 643 for SK08_227, a second allele-specific forward primer represented by SEQ ID NO: 644, and a reverse sequence represented by SEQ ID NO: 645. The anti-primer set, the first allele-specific forward primer represented by SEQ ID NO: 646 for SK08_233, the second allele-specific forward primer represented by SEQ ID NO: 647, and the reverse primer set represented by SEQ ID NO: 648, the sequence for SK08_241 A first allele-specific forward primer represented by SEQ ID NO: 649, a second allele-specific forward primer represented by SEQ ID NO: 650, and a reverse primer set represented by SEQ ID NO: 651, the first allele represented by SEQ ID NO: 652 for SK08_254 A set of trait-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 653 and a reverse primer set represented by SEQ ID NO: 654, a first allele-specific forward primer represented by SEQ ID NO: 655 for SK08_260, SEQ ID NO: 656 A second allele-specific forward primer represented by and a reverse primer set represented by SEQ ID NO: 657, a first allele-specific forward primer represented by SEQ ID NO: 658 for SK09_004, and a second allele-specific represented by SEQ ID NO: 659 A forward primer and a reverse primer set represented by SEQ ID NO: 660, a first allele-specific forward primer represented by SEQ ID NO: 661 for SK09_028, a second allele-specific forward primer represented by SEQ ID NO: 662, and SEQ ID NO: 663 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 664 for SK09_035, a second allele-specific forward primer represented by SEQ ID NO: 665, and a reverse primer set represented by SEQ ID NO: 666, to SK09_042 The first allele-specific forward primer represented by SEQ ID NO: 667, the second allele-specific forward primer represented by SEQ ID NO: 668, and the reverse primer set represented by SEQ ID NO: 669, the first allele-specific forward primer represented by SEQ ID NO: 670 for SK09_052 1 allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 671 and the reverse primer set represented by SEQ ID NO: 672, the first allele-specific forward primer represented by SEQ ID NO: 673 for SK09_056, sequence The second allele-specific forward primer represented by SEQ ID NO: 674 and the reverse primer set represented by SEQ ID NO: 675, the first allele-specific forward primer represented by SEQ ID NO: 676 for SK09_062, the second allele represented by SEQ ID NO: 677 A trait-specific forward primer and a reverse primer set represented by SEQ ID NO: 678, a first allele-specific forward primer represented by SEQ ID NO: 679 for SK09_068, a second allele-specific forward primer represented by SEQ ID NO: 680, and SEQ ID NO: 681 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 682 for SK09_075, a second allele-specific forward primer represented by SEQ ID NO: 683, and a reverse primer set represented by SEQ ID NO: 684, A set of a first allele-specific forward primer represented by SEQ ID NO: 685 for SK09_093, a second allele-specific forward primer represented by SEQ ID NO: 686 and a reverse primer set represented by SEQ ID NO: 687, SEQ ID NO: 688 for SK09_123 A set of first allele-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 689 and a reverse primer set represented by SEQ ID NO: 690, the first allele-specific forward primer represented by SEQ ID NO: 691 for SK09_132 , the second allele-specific forward primer represented by SEQ ID NO: 692 and the reverse primer set represented by SEQ ID NO: 693, the first allele-specific forward primer represented by SEQ ID NO: 694 for SK09_140, the first allele-specific forward primer represented by SEQ ID NO: 695 2 allele-specific forward primers and reverse primer set represented by SEQ ID NO: 696, the first allele-specific forward primer represented by SEQ ID NO: 697 for SK09_154, the second allele-specific forward primer represented by SEQ ID NO: 698 and sequence Reverse primer set represented by No. 699, the first allele-specific forward primer represented by SEQ ID NO: 700 for SK09_162, the second allele-specific forward primer represented by SEQ ID NO: 701, and the reverse primer represented by SEQ ID NO: 702 Set, a first allele specific forward primer represented by SEQ ID NO: 703 for SK09_160, a second allele specific forward primer represented by SEQ ID NO: 704 and a reverse primer set represented by SEQ ID NO: 705, SEQ ID NO: 706 for SK09_169 The first allele-specific forward primer represented by SEQ ID NO: 707, the second allele-specific forward primer represented by SEQ ID NO: 708, and the reverse primer set represented by SEQ ID NO: 708, the first allele-specific represented by SEQ ID NO: 709 for SK09_173 Forward primer, second allele-specific forward primer represented by SEQ ID NO: 710 and reverse primer set represented by SEQ ID NO: 711, first allele-specific forward primer represented by SEQ ID NO: 712 for SK09_177, SEQ ID NO: 713 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 714, a first allele-specific forward primer represented by SEQ ID NO: 715 for SK09_181, and a second allele-specific forward primer represented by SEQ ID NO: 716 and a reverse primer set represented by SEQ ID NO: 717, a first allele-specific forward primer represented by SEQ ID NO: 718 for SK09_186, a second allele-specific forward primer represented by SEQ ID NO: 719, and a reverse sequence represented by SEQ ID NO: 720. The anti-primer set, the first allele-specific forward primer represented by SEQ ID NO: 721 for SK09_189, the second allele-specific forward primer represented by SEQ ID NO: 722, and the reverse primer set represented by SEQ ID NO: 723, the sequence for SK09_197 The first allele-specific forward primer represented by SEQ ID NO: 724, the second allele-specific forward primer represented by SEQ ID NO: 725, and the reverse primer set represented by SEQ ID NO: 726, the first allele represented by SEQ ID NO: 727 for SK09_208 Trait specific forward primer, second allele specific forward primer represented by SEQ ID NO: 728 and reverse primer set represented by SEQ ID NO: 729, first allele specific forward primer represented by SEQ ID NO: 730 for SK09_213, SEQ ID NO: 731 A second allele-specific forward primer represented by and a reverse primer set represented by SEQ ID NO: 732, a first allele-specific forward primer represented by SEQ ID NO: 733 for SK09_224, and a second allele-specific represented by SEQ ID NO: 734 A forward primer and a reverse primer set represented by SEQ ID NO: 735, a first allele-specific forward primer represented by SEQ ID NO: 736 for SK09_228, a second allele-specific forward primer represented by SEQ ID NO: 737, and SEQ ID NO: 738 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 739 for SK10_005, a second allele-specific forward primer represented by SEQ ID NO: 740, and a reverse primer set represented by SEQ ID NO: 741, SK10_011 The first allele-specific forward primer represented by SEQ ID NO: 742, the second allele-specific forward primer represented by SEQ ID NO: 743, and the reverse primer set represented by SEQ ID NO: 744, the first allele-specific forward primer represented by SEQ ID NO: 745 for SK10_014 1 allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 746 and the reverse primer set represented by SEQ ID NO: 747, the first allele-specific forward primer represented by SEQ ID NO: 748 for SK10_019, sequence The second allele-specific forward primer represented by No. 749 and the reverse primer set represented by SEQ ID NO: 750, the first allele-specific forward primer represented by SEQ ID NO: 751 for SK10_028, the second allele represented by SEQ ID NO: 752 A trait-specific forward primer and a reverse primer set represented by SEQ ID NO: 753, a first allele-specific forward primer represented by SEQ ID NO: 754 for SK10_032, a second allele-specific forward primer represented by SEQ ID NO: 755, and SEQ ID NO: 756 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 757 for SK10_038, a second allele-specific forward primer represented by SEQ ID NO: 758, and a reverse primer set represented by SEQ ID NO: 759, A first allele-specific forward primer represented by SEQ ID NO: 760 for SK10_046, a second allele-specific forward primer represented by SEQ ID NO: 761 and a reverse primer set represented by SEQ ID NO: 762, SEQ ID NO: 763 for SK10_053 A set of first allele-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 764 and a reverse primer set represented by SEQ ID NO: 765, the first allele-specific forward primer represented by SEQ ID NO: 766 for SK10_061 , the second allele-specific forward primer represented by SEQ ID NO: 757 and the reverse primer set represented by SEQ ID NO: 768, the first allele-specific forward primer represented by SEQ ID NO: 769 for SK10_076, the first allele-specific forward primer represented by SEQ ID NO: 770 2 allele-specific forward primers and reverse primer set represented by SEQ ID NO: 771, the first allele-specific forward primer represented by SEQ ID NO: 772 for SK10_086, the second allele-specific forward primer represented by SEQ ID NO: 773 and sequence Reverse primer set represented by No. 774, the first allele-specific forward primer represented by SEQ ID NO: 775 for SK10_092, the second allele-specific forward primer represented by SEQ ID NO: 776, and the reverse primer represented by SEQ ID NO: 777 Set, a first allele specific forward primer represented by SEQ ID NO: 778 for SK10_097, a second allele specific forward primer represented by SEQ ID NO: 779 and a reverse primer set represented by SEQ ID NO: 780, SEQ ID NO: 781 for SK10_113 The first allele-specific forward primer represented by SEQ ID NO: 782, the second allele-specific forward primer represented by SEQ ID NO: 783, and the reverse primer set represented by SEQ ID NO: 783, the first allele-specific represented by SEQ ID NO: 784 for SK10_123 Forward primer, a second allele-specific forward primer represented by SEQ ID NO: 785 and a set of reverse primers represented by SEQ ID NO: 786, a first allele-specific forward primer represented by SEQ ID NO: 787 for SK10_128, SEQ ID NO: 788 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 789, a first allele-specific forward primer represented by SEQ ID NO: 790 for SK10_131, and a second allele-specific forward primer represented by SEQ ID NO: 791 and a reverse primer set represented by SEQ ID NO: 792, a first allele-specific forward primer represented by SEQ ID NO: 793 for SK10_135, a second allele-specific forward primer represented by SEQ ID NO: 794, and a reverse sequence represented by SEQ ID NO: 795. The anti-primer set, the first allele-specific forward primer represented by SEQ ID NO: 796 for SK10_146, the second allele-specific forward primer represented by SEQ ID NO: 797, and the reverse primer set represented by SEQ ID NO: 798, the sequence for SK10_153 The first allele-specific forward primer represented by SEQ ID NO: 799, the second allele-specific forward primer represented by SEQ ID NO: 800, and the reverse primer set represented by SEQ ID NO: 801, the first allele represented by SEQ ID NO: 802 for SK10_161 Trait specific forward primer, second allele specific forward primer represented by SEQ ID NO: 803 and reverse primer set represented by SEQ ID NO: 804, first allele specific forward primer represented by SEQ ID NO: 805 for SK10_167, SEQ ID NO: 806 A second allele-specific forward primer represented by and a reverse primer set represented by SEQ ID NO: 807, a first allele-specific forward primer represented by SEQ ID NO: 808 for SK10_174, and a second allele-specific represented by SEQ ID NO: 809 A forward primer and a reverse primer set represented by SEQ ID NO: 810, a first allele-specific forward primer represented by SEQ ID NO: 811 for SK10_182, a second allele-specific forward primer represented by SEQ ID NO: 812, and SEQ ID NO: 813 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 814 for SK11_001, a second allele-specific forward primer represented by SEQ ID NO: 815, and a reverse primer set represented by SEQ ID NO: 816, SK11_007 The first allele-specific forward primer represented by SEQ ID NO: 817, the second allele-specific forward primer represented by SEQ ID NO: 818, and the reverse primer set represented by SEQ ID NO: 819, the first allele-specific forward primer represented by SEQ ID NO: 820 for SK11_015 1 allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 821 and the reverse primer set represented by SEQ ID NO: 822, the first allele-specific forward primer represented by SEQ ID NO: 823 for SK11_022, sequence The second allele-specific forward primer represented by SEQ ID NO: 824 and the reverse primer set represented by SEQ ID NO: 825, the first allele-specific forward primer represented by SEQ ID NO: 826 for SK11_027, the second allele represented by SEQ ID NO: 827 A trait-specific forward primer and a set of reverse primers represented by SEQ ID NO: 828, a first allele-specific forward primer represented by SEQ ID NO: 829 for SK11_035, a second allele-specific forward primer represented by SEQ ID NO: 830, and SEQ ID NO: 831 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 832 for SK11_042, a second allele-specific forward primer represented by SEQ ID NO: 833, and a reverse primer set represented by SEQ ID NO: 834, A set of a first allele-specific forward primer represented by SEQ ID NO: 835 for SK11_050, a second allele-specific forward primer represented by SEQ ID NO: 836 and a reverse primer set represented by SEQ ID NO: 837, SEQ ID NO: 838 for SK11_059 A set of first allele-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 839 and a reverse primer set represented by SEQ ID NO: 840, the first allele-specific forward primer represented by SEQ ID NO: 841 for SK11_066 , the second allele-specific forward primer represented by SEQ ID NO: 842 and the reverse primer set represented by SEQ ID NO: 843, the first allele-specific forward primer represented by SEQ ID NO: 844 for SK11_073, the first allele-specific forward primer represented by SEQ ID NO: 845 2 allele-specific forward primers and reverse primer set represented by SEQ ID NO: 846, the first allele-specific forward primer represented by SEQ ID NO: 847 for SK11_084, the second allele-specific forward primer represented by SEQ ID NO: 848 and sequence Reverse primer set represented by No. 849, the first allele-specific forward primer represented by SEQ ID NO: 850 for SK11_092, the second allele-specific forward primer represented by SEQ ID NO: 851, and the reverse primer represented by SEQ ID NO: 852 Set, a first allele specific forward primer represented by SEQ ID NO: 853 for SK11_102, a second allele specific forward primer represented by SEQ ID NO: 854 and a reverse primer set represented by SEQ ID NO: 855, SEQ ID NO: 856 for SK11_109 The first allele-specific forward primer represented by SEQ ID NO: 857, the second allele-specific forward primer represented by SEQ ID NO: 858, and the reverse primer set represented by SEQ ID NO: 858, the first allele-specific represented by SEQ ID NO: 859 for SK11_117 Forward primer, a second allele-specific forward primer represented by SEQ ID NO: 860 and a set of reverse primers represented by SEQ ID NO: 861, a first allele-specific forward primer represented by SEQ ID NO: 862 for SK11_125, SEQ ID NO: 863 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 864, a first allele-specific forward primer represented by SEQ ID NO: 865 for SK11_130, and a second allele-specific forward primer represented by SEQ ID NO: 866 and a set of reverse primers represented by SEQ ID NO: 867, a first allele-specific forward primer represented by SEQ ID NO: 868 for SK11_137, a second allele-specific forward primer represented by SEQ ID NO: 869, and a reverse sequence represented by SEQ ID NO: 870. The anti-primer set, the first allele-specific forward primer represented by SEQ ID NO: 871 for SK12_007, the second allele-specific forward primer represented by SEQ ID NO: 872, and the reverse primer set represented by SEQ ID NO: 873, the sequence for SK12_019 A set of first allele-specific forward primers represented by SEQ ID NO: 874, second allele-specific forward primers represented by SEQ ID NO: 875 and reverse primers represented by SEQ ID NO: 876, the first allele represented by SEQ ID NO: 877 for SK12_022 A set of trait-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 878 and a set of reverse primers represented by SEQ ID NO: 879, a first allele-specific forward primer represented by SEQ ID NO: 880 for SK12_033, SEQ ID NO: 881 A second allele-specific forward primer represented by and a reverse primer set represented by SEQ ID NO: 882, a first allele-specific forward primer represented by SEQ ID NO: 883 for SK12_042, and a second allele-specific represented by SEQ ID NO: 884 A forward primer and a reverse primer set represented by SEQ ID NO: 885, a first allele-specific forward primer represented by SEQ ID NO: 886 for SK12_052, a second allele-specific forward primer represented by SEQ ID NO: 887, and SEQ ID NO: 888 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 889 for SK12_066, a second allele-specific forward primer represented by SEQ ID NO: 890, and a reverse primer set represented by SEQ ID NO: 891, SK12_073 The first allele-specific forward primer represented by SEQ ID NO: 892, the second allele-specific forward primer represented by SEQ ID NO: 893, and the reverse primer set represented by SEQ ID NO: 894, the first allele-specific forward primer represented by SEQ ID NO: 895 for SK12_080 1 allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 896 and the reverse primer set represented by SEQ ID NO: 897, the first allele-specific forward primer represented by SEQ ID NO: 898 for SK12_089, sequence The second allele-specific forward primer represented by No. 899 and the reverse primer set represented by SEQ ID NO: 900, the first allele-specific forward primer represented by SEQ ID NO: 901 for SK12_096, the second allele represented by SEQ ID NO: 902 A trait-specific forward primer and a reverse primer set represented by SEQ ID NO: 903, a first allele-specific forward primer represented by SEQ ID NO: 904 for SK12_110, a second allele-specific forward primer represented by SEQ ID NO: 905, and SEQ ID NO: 906 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 907 for SK12_118, a second allele-specific forward primer represented by SEQ ID NO: 908, and a reverse primer set represented by SEQ ID NO: 909, A first allele-specific forward primer represented by SEQ ID NO: 910 for SK12_123, a second allele-specific forward primer represented by SEQ ID NO: 911 and a reverse primer set represented by SEQ ID NO: 912, SEQ ID NO: 913 for SK12_128 A set of first allele-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 914 and a reverse primer set represented by SEQ ID NO: 915, the first allele-specific forward primer represented by SEQ ID NO: 916 for SK12_134 , the second allele-specific forward primer represented by SEQ ID NO: 917 and the reverse primer set represented by SEQ ID NO: 918, the first allele-specific forward primer represented by SEQ ID NO: 919 for SK12_138, the first allele-specific forward primer represented by SEQ ID NO: 920 2 allele-specific forward primers and reverse primer set represented by SEQ ID NO: 921, the first allele-specific forward primer represented by SEQ ID NO: 922 for SK12_147, the second allele-specific forward primer represented by SEQ ID NO: 923, and the sequence Reverse primer set represented by No. 924, the first allele-specific forward primer represented by SEQ ID NO: 925 for SK12_151, the second allele-specific forward primer represented by SEQ ID NO: 926, and the reverse primer represented by SEQ ID NO: 927 Set, first allele specific forward primer represented by SEQ ID NO: 928 to SK12_161, second allele specific forward primer represented by SEQ ID NO: 929 and reverse primer set represented by SEQ ID NO: 930, SEQ ID NO: 931 to SK13_003 The first allele-specific forward primer represented by SEQ ID NO: 932, the second allele-specific forward primer represented by SEQ ID NO: 933, and the reverse primer set represented by SEQ ID NO: 933, the first allele-specific represented by SEQ ID NO: 934 for SK13_008 Forward primer, a second allele-specific forward primer represented by SEQ ID NO: 935 and a set of reverse primers represented by SEQ ID NO: 936, a first allele-specific forward primer represented by SEQ ID NO: 937 for SK13_015, SEQ ID NO: 938 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 939, a first allele-specific forward primer represented by SEQ ID NO: 940 for SK13_023, and a second allele-specific forward primer represented by SEQ ID NO: 941 and a reverse primer set represented by SEQ ID NO: 942, a first allele-specific forward primer represented by SEQ ID NO: 943 for SK13_055, a second allele-specific forward primer represented by SEQ ID NO: 944, and a reverse sequence represented by SEQ ID NO: 945. The anti-primer set, the first allele-specific forward primer represented by SEQ ID NO: 946 for SK13_061, the second allele-specific forward primer represented by SEQ ID NO: 947, and the reverse primer set represented by SEQ ID NO: 948, the sequence for SK13_067 The first allele-specific forward primer represented by SEQ ID NO: 949, the second allele-specific forward primer represented by SEQ ID NO: 950, and the reverse primer set represented by SEQ ID NO: 951, the first allele represented by SEQ ID NO: 952 for SK13_071 A set of trait-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 953 and a reverse primer set represented by SEQ ID NO: 954, a first allele-specific forward primer represented by SEQ ID NO: 955 for SK13_080, SEQ ID NO: 956 The second allele-specific forward primer represented by and the reverse primer set represented by SEQ ID NO: 957, the first allele-specific forward primer represented by SEQ ID NO: 958 for SK13_088, the second allele-specific represented by SEQ ID NO: 959 A forward primer and a reverse primer set represented by SEQ ID NO: 960, a first allele-specific forward primer represented by SEQ ID NO: 961 for SK13_094, a second allele-specific forward primer represented by SEQ ID NO: 962, and SEQ ID NO: 963 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 964 for SK13_103, a second allele-specific forward primer represented by SEQ ID NO: 965, and a reverse primer set represented by SEQ ID NO: 966, SK13_107 A set of the first allele-specific forward primer represented by SEQ ID NO: 967, the second allele-specific forward primer represented by SEQ ID NO: 968, and the reverse primer set represented by SEQ ID NO: 969, the first allele-specific forward primer represented by SEQ ID NO: 970 for SK13_113 1 allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 971 and the reverse primer set represented by SEQ ID NO: 972, the first allele-specific forward primer represented by SEQ ID NO: 973 for SK13_123, sequence The second allele-specific forward primer represented by No. 974 and the reverse primer set represented by SEQ ID NO: 975, the first allele-specific forward primer represented by SEQ ID NO: 976 for SK13_127, the second allele represented by SEQ ID NO: 977 A trait-specific forward primer and a set of reverse primers represented by SEQ ID NO: 978, a first allele-specific forward primer represented by SEQ ID NO: 979 for SK13_131, a second allele-specific forward primer represented by SEQ ID NO: 980, and SEQ ID NO: 981 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 982 for SK13_138, a second allele-specific forward primer represented by SEQ ID NO: 983, and a reverse primer set represented by SEQ ID NO: 984, A set of first allele-specific forward primers represented by SEQ ID NO: 985 for SK13_150, second allele-specific forward primers represented by SEQ ID NO: 986 and reverse primers represented by SEQ ID NO: 987, SEQ ID NO: 988 for SK13_157 A first allele-specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 989, and a reverse primer set represented by SEQ ID NO: 990, the first allele-specific forward primer represented by SEQ ID NO: 991 for SK13_164 , a second allele-specific forward primer represented by SEQ ID NO: 992 and a primer set selected from the group consisting of a reverse primer set represented by SEQ ID NO: 993, and may specifically include 19 or more primer sets. .

Another aspect of the present invention provides a KASP marker kit for genetic mapping of sesame varieties, including the primer set.

In the kit of the present invention, other than the primer set, reagents for performing an amplification reaction may be further included, and specifically, DNA polymerase, DNA polymerase cofactors, dNTPs, buffers, and the like may be included.

Another aspect of the present invention provides a primer set for determining sesame variety, including 19 or more primer sets selected from the group consisting of 331 primer sets consisting of oligonucleotides of SEQ ID NO: 1 to SEQ ID NO: 993.

In the present invention, "sesame" is one of the world's representative oil crops, including Korea, and is mainly consumed as sesame oil, and is also widely used as a food ingredient for sesame salt and desserts such as sesame seeds. Recently, oleic acid, a high-quality fatty acid contained in sesame seeds, is used. Various functional ingredients such as linoleic acid, antioxidant sesamin, and lecithin, which are good for the brain, have been reported and are widely illuminated as health functional foods. , Gopum, Geumok, Nambaek, Namsan, Naman, Nuri, Dasak, Daheuk, Dubeol, Mangeum, Manheuk, Miheuk, Milseong, Sangbaek, Suwon, Soonheuk, Annam, Ansan, Yangheuk, Osan, Yumi, Yuseong, Yunheuk, Jungmo 5002, It may include Jungmo 5003, Jungmo 5005, Jingi, Jinbaek, Jinyul, Jinju, Chamhwang, Pyeongan, Pungnam, Poongsan, Pungan, Hansan, Handsome, Hwaheuk, Hwangbaek, and Heukseon.

The description of the primer set in the present invention is as described above.

In another aspect of the present invention, a composition for determining sesame variety including the primer set for determining sesame variety may be provided.

The composition for discrimination includes 19 or more primer sets selected from the group consisting of 331 primer sets consisting of oligonucleotides of SEQ ID NO: 1 to SEQ ID NO: 993.

The description of “primer” and “primer set” in the present invention is as described above.

In another aspect of the present invention, a kit for determining a sesame variety including the composition for determining a sesame variety may be provided.

The kit for discrimination of the present invention may be a kit including essential elements necessary for performing PCR analysis or multiplex-PCR analysis in addition to the composition for determining the sesame variety. The kit includes, in addition to each set of primers specific for each polynucleotide, a test tube or other suitable container, a reaction buffer (with varying pH and magnesium concentration), deoxynucleotides (dNTPs), an enzyme such as Taq-polymerase. , DNase, RNAse inhibitors, DEPC-water, sterile water, and the like. In addition, the kit of the present invention may additionally include components required for performing electrophoresis capable of confirming the amplification of PCR products or identification criteria for known species.

In addition, the present invention provides a method for determining a sesame variety using genomic DNA isolated from a sesame sample and the primer set.

The method specifically includes: (a) isolating genomic DNA from a sesame seed sample; (b) performing KASP using the genomic DNA of step (a) as a template and using the oligonucleotide primer set of the present invention; and (c) analyzing the amplification product of the KASP.

The method of the present invention includes the step of isolating genomic DNA from a sesame seed sample. As a method for isolating genomic DNA from the sesame seed sample, a method known in the art may be used, and an ethanol precipitation method followed by phenol:chloroform extraction may be used. A target sequence may be amplified by performing an amplification reaction by adding DNA polymerase using the isolated genomic DNA as a template and using the oligonucleotide primer set according to an embodiment of the present invention as a primer.

Methods for amplifying a target nucleic acid include polymerase chain reaction (PCR), ligase chain reaction, nucleic acid sequence-based amplification, transcription-based amplification system, Strand displacement amplification or amplification via Qβ replicase or any other suitable method for amplifying nucleic acid molecules known in the art. In the above, PCR is a method of amplifying a target nucleic acid from a pair of primers that specifically bind to the target nucleic acid using a polymerase. Such PCR methods are well known in the art, and commercially available kits may be used.

In the method of the present invention, the amplified target sequence may be labeled with a detectable labeling substance. Specifically, the label material may be a material that emits fluorescence, phosphorescence or radioactivity, but is not limited thereto. Preferably, the labeling material may be FAM, HEX, VIC, JOE, ROX, TAMRA, Cy3 or Cy5. When the target sequence is amplified, PCR is performed by labeling the labeling material at the 5'-end of the primer, and the target sequence can be labeled with a detectable fluorescent labeling material. The oligonucleotide primer set used to amplify the target sequence is as described above.

As used herein, the term "amplification reaction" means a reaction for amplifying a nucleic acid molecule. A variety of amplification reactions have been reported in the art, including polymerase chain reaction (PCR) (U.S. Pat. Nos. 4,683,195, 4,683,202, and 4,800,159), reverse transcription-polymerase chain reaction (RT-PCR) (Sambrook et al., Molecular Cloning. A Laboratory Manual, 3rd ed. Cold Spring Harbor Press (2001)), the method of Miller, H. I. (WO 89/06700) and Davey, C. et al. (EP 329,822), ligase chain reaction (LCR), Gap-LCR (WO 90/01069), repair chain reaction (EP 439,182), transcription-mediated amplification (TMA) (WO 88/10315), selfsustained sequence replication) (WO 90/06995), selective amplification of target polynucleotide sequences (US Patent No. 6,410,276), consensus sequence primed polymerase chain reaction (CPPCR) (U.S. Patent No. 4,437,975), arbitrarily primed polymerase chain reaction (AP-PCR) (U.S. Patent Nos. 5,413,909 and 5,861,245), nucleic acid sequence based amplification (NASBA) ) (US Pat. Nos. 5,130,238, 5,409,818, 5,554,517, and 6,063,603), strand displacement amplification and loopmediated isothermal amplification; LAMP), but is not limited thereto. Other amplification methods that may be used are described in U.S. Patent Nos. 5,242,794, 5,494,810, 4,988,617 and U.S. Patent No. 09/854,317.

According to one embodiment of the present invention, using the 331 KASP markers of the present invention, it is possible to create a sesame gene association map, through which excellent genes can be discovered, and through Marker-Assisted Selection (MAS) Sesame molecular breeding can be activated.

In addition, genotypic profiling information on 46 domestic sesame varieties can be used to determine whether domestic sesame is mixed or authentic. In particular, when using 19 KASP markers selected as key markers, a large amount of samples can be obtained faster and at lower cost than conventional methods. can be efficiently analyzed.

In the future, 331 KASP markers are expected to be used for analysis of genetic resource relatedness of sesame seeds and classification of domestic and imported varieties.

Figure 1 shows information produced after resequencing of 12 varieties of sesame seeds and information obtained by preprocessing these data before mutation analysis.
Figure 2 shows the number of genetic mutations (SNPs, InDel) searched for each chromosome after mapping the sesame genome information of 12 varieties to the reference genome. They were analyzed by dividing them into multi-allele, heterozygous di-allele, and homozygous di-allele, and the numbers of homozygous di-allele were divided and displayed by PIC range.
Figure 3 shows examples of successful and unsuccessful analyzes when the KASP assay was analyzed on 46 varieties of sesame.
4 is a physical map created using the chromosomal locations of the final 331 KASP markers that were successfully analyzed. The physical distance unit is Mbp.
5 is a phylogenetic tree of 46 domestic sesame varieties prepared using 331 KASP marker profiling information.
6 is a profiling image of key markers selected as a minimum to classify 46 domestic sesame varieties and a phylogenetic tree of 46 varieties created using them.
Figure 7 is a list of 331 KASP markers.

Hereinafter, the present invention will be described in more detail through examples. These examples are intended to explain the present invention in more detail, and the scope of the present invention is not limited by these examples.

Example 1: Analysis of genome information of domestic varieties of sesame seeds

In order to secure the genome information of 12 domestic varieties of sesame seeds, resequencing was performed. Nucleotide sequences were mass-produced in each cultivar using Illumina Hiseq X equipment (FIG. 1). DNA for sequencing was extracted using DNeasy Plant Mini Kit (QIAGEN, Hilden, Germany).

Information analysis of the produced genome sequence was performed using open genome information analysis programs.

(Pre-processing) Genome read sequences for each breed produced from llumina equipment were pre-processed to remove low-quality nucleotide sequence regions. The software used was Trimmomatic ver. 0.33, the option was to remove Phred score of 20 or less, and the minimum length of the read remaining after removal was 50 bases or more. (Standard genome) The sesame reference genome used to search for genetic variation by cultivar is the Zhongzhi13 genome sequence already reported in China. Variations were searched for by mapping the preprocessed read sequences for each breed to the standard genome. (Mapping and mutation search) Mapping was performed using the BWA 0.7.17 program, and the mapping information for each breed was Picard ver. 2.20.4 and GATK ver. Genotypes were analyzed using the 3.5 program. Samtools ver. Based on the 1.9 program, data for accurate genotyping were produced. (Conditions for mutation search) To find mutations, the following mutation filtering conditions were applied. [Variation filtering conditions: min coverage: >=5, max coverage: <=250, Quality: >=20, SNP ratio: 0.9, Progeny: 12] The meaning of SNP ratio 0.9 is that, for example, SNP “A” is the corresponding position If there are 100 base sequence reads mapped to, “A” means that it is found in more than 90 reads and the accuracy (more than 90%) that other bases are found in the remaining reads. Progeny 12 means that genome sequence reads derived from all 12 sesame varieties must be present at the location mapped to the reference genome, and SNP or InDel mutations must be found. Progeny 12 was selected as the most stringent standard to compare genetic variants of 12 sequenced sesame varieties.

Through such genome information production and analysis, 1,059,977 genetic mutations (SNPs, InDel) were discovered among 12 varieties of sesame seeds. The distribution of genetic variation for each chromosome is shown in FIG. 2 . Chromosome 13 was the least with 38,650 and chromosome 3 was the most with 156,521.

Example 2: Sesame genetic mutation selection and KASP marker production

Genetic variants with high polymorphism among about 1.06 million genetic variants were selected to develop general-purpose molecular markers that can be used for various purposes such as variety, lineage, and genetic resources, variety identification, genetic mapping, and genetic resource analysis. selected. First, 7,232 multi-alleles and 20,280 heterozygous di-alleles that could not be used as KASP markers were excluded, and polymorphism information content (PIC) values were calculated for 1,032,465 homozygous di-alleles (Fig. 2). PIC is a polymorphism index of a marker and has a value of 0 to 0.5, and the higher the polymorphism, the higher the PIC value and the higher the usability as a marker. It is calculated by Equation 1 below.

[Equation 1]

(Pi는 I번째 유전자형의 확률)

(Pi is the probability of the Ith genotype)

Among the 267,286 variants with a PIC of 0.4 or higher, about 500 genetic variants separated by 700kb ~ 800kb intervals in the chromosome were selected, and after checking whether there were other genetic variants within 100bp on either side of these variants through IGV (Integrative Genomics Viewer), 396 variants with no other variants were selected. was produced as the final KASP marker. KASP primer design was performed by extracting 100 bp nucleotide sequences on both sides of each mutation. KASP primers were prepared by LGC genomics (London, UK).

Example 3: Sesame KASP marker development

Genotyping was performed on 46 varieties of domestic sesame seeds using the 396 KASP assay sets produced. Two individuals for each breed were analyzed.

Genotyping of KASP markers was performed using the Nexar system (LGC Douglas Scientific, Alexandria, USA) at the Seed Industry Promotion Center of the Agricultural Technology Commercialization Foundation. At this time, the composition of the reaction solution was 1.6 μL in total, including 0.8 μL of 2× KASP Master Mix (LGC Genomics, London, UK), 0.02 μL of KASP assay mix (primer mix), and 5 ng of genomic DNA template. And, the thermal cycling profile is shown in Table 1 below. The PCR reaction was performed twice for each sample.

PCR step temperature hour cycle Step 1
(Hot-start activation) 94℃ 15 minutes x 1 cycle Step 2
(Touchdown) 94℃ 20 seconds x 10 cycles 61-55℃
(0.6 ℃ drop per cycle) 60 seconds Step 3
(Amplification) 94℃ 20 seconds x 26 cycles 55℃ 60 seconds additional step 4
(Recycling) 94℃ 20 seconds x 6 cycles 57℃ 60 seconds

Of the 396 KASP assays, 65 failed amplification or were amplified only in some varieties, and the final 331 assays were stably amplified in all 46 varieties and showed clear polymorphisms between alleles, which could be used as markers (FIG. 3).

The list of 331 finally successful KASP markers is shown in Table 2 and FIG. 7, and the physical map using their chromosomal locations is shown in FIG. Genotype information profiling these markers in 46 breeds is shown in Table 3.

marker name chromosome Location (bp) SNP or InDel First allele-specific forward primer sequence (FAM) Second allele specific forward primer sequence (FAM) reverse primer sequence SK01_007 chr01 658,676 G/A ACATGGACGTCTAACAAGAGCTTG (SEQ ID NO: 1) ACATGGACGTCTAACAAGAGCTTA (SEQ ID NO: 2) CTGCGATATATATTGGTACCTACTGTGAA (SEQ ID NO: 3) SK01_012 chr01 1,172,155 A/G ATTCACAACCCCAAAAAACAAAAGTGAAT (SEQ ID NO: 4) CACAACCCCAAAAAACAAAAGTGAAC (SEQ ID NO: 5) TTGCATGTGGATGATGTTATGTCAGTGAT (SEQ ID NO: 6) SK01_018 chr01 1,753,423 A/G AAAACTCAGCTGGGATTCTTCCTGT (SEQ ID NO: 7) ACTCAGCTGGGATTCTTCCTGC (SEQ ID NO: 8) GAAGCTCACTTCTCTTAACCTTTTCATCAA (SEQ ID NO: 9) SK01_025 chr01 2,462,317 T/C GAATACCATTTGTAACGGATTTATATACGA (SEQ ID NO: 10) AATACCATTTGTAACGGATTTATATACGG (SEQ ID NO: 11) GTAGTTATCCCTAACATAAATGACATCGTA (SEQ ID NO: 12) SK01_030 chr01 3,030,275 G/T ATCGTACAACCATCCAACCAGTAC (SEQ ID NO: 13) GATCGTACAACCATCCAACCAGTAA (SEQ ID NO: 14) GGGAGGTTAATGAGCTAGGTCGTTA (SEQ ID NO: 15) SK01_038 chr01 3,831,307 C/T ACTGCATATTTGCTATGTTACTACTGC (SEQ ID NO: 16) GACTGCATATTTGCTATGTTACTACTGT (SEQ ID NO: 17) GTGGTATGATGTACTCATGGTTGTTGATT (SEQ ID NO: 18) SK01_046 chr01 4,586,274 G/A TGTTACAGGCACCAGTAAAGCC (SEQ ID NO: 19) GTTGTTACAGGCACCAGTAAAGCT (SEQ ID NO: 20) TAAATGGAAGGGGACGTGCGAGTT (SEQ ID NO: 21) SK01_054 chr01 5,413,430 G/A ATCAATTTGGGTGGACTGGTTGTAC (SEQ ID NO: 22) CATCAATTTGGGTGGACTGGTTGTAT (SEQ ID NO: 23) GTCCAAACATAGGTGTTTTGTAACCGATT (SEQ ID NO: 24) SK01_070 chr01 6,979,952 C/T CTTTGCTAGTGTTATACCAGGACC (SEQ ID NO: 25) ATCTTTGCTAGTGTTATACCAGGACT (SEQ ID NO: 26) CATCAACAAATACGGTGAGACATCTACAA (SEQ ID NO: 27) SK01_084 chr01 8,405,383 G/C TTTTCGTCGATTCTGAGACGAGG (SEQ ID NO: 28) TTTCGTCGATTCTGAGACGAGC (SEQ ID NO: 29) AACTCGATGCAAAGAATCAGCGAATTCAAA (SEQ ID NO: 30) SK01_093 chr01 9,310,043 G/A TCAGGGATGTGTGAAGGGAGC (SEQ ID NO: 31) TCAGGGATGTGTGAAGGGAGT (SEQ ID NO: 32) AGCAATACAAAAAACCAGAAGCACCTCTT (SEQ ID NO: 33) SK01_102 chr01 10,179,573 G/A ACCTTTGTGCAACTTTTGTTTTAGTTTTG (SEQ ID NO: 34) GACCTTTGTGCAACTTTTGTTTTAGTTTTA (SEQ ID NO: 35) CTCAGACAAGCCTTTTGTGCATATAAGTT (SEQ ID NO: 36) SK01_109 chr01 10,869,404 T/C GTTGATCATCAATCTATGCAGACACT (SEQ ID NO: 37) GTTGATCATCAATCTATGCAGACACC (SEQ ID NO: 38) CCCCTCTTGTGAGAAGAGTTAGATTTTAA (SEQ ID NO: 39) SK01_113 chr01 11,322,844 C/T CAACCCAACGGACTGACCCG (SEQ ID NO: 40) ACAACCCAACGGACTGACCCA (SEQ ID NO: 41) AAGAAAAAGGGTTTCTGTTTTCGTGAGAAT (SEQ ID NO: 42) SK01_120 chr01 11,980,332 T/C AATAATGTTGAAACTAGTATGATCAATAGTA (SEQ ID NO: 43) AATAATGTTGAAACTAGTATGATCAATAGTG (SEQ ID NO: 44) CAGTTTCCAGGCTTGTTGAAATTGGTATA (SEQ ID NO: 45) SK01_128 chr01 12,760,634 A/G GGGAGTGAAGTCATCGTGTCGTT (SEQ ID NO: 46) GGAGTGAAGTCATCGTGTCGTC (SEQ ID NO: 47) AGCAGCAGATGAGGACTCCCAATT (SEQ ID NO: 48) SK01_133 chr01 13,300,693 T/C GGCTTATGCTTGCACAGCCGATA (SEQ ID NO: 49) GCTTATGCTTGCACAGCCGATG (SEQ ID NO: 50) GCTATTGAAAATCTCAAGAACTCCAGCAA (SEQ ID NO: 51) SK01_139 chr01 13,877,193 G/A GTCCGATTTTTAGTTTGAGGTATCTTAC (SEQ ID NO: 52) GGTCCGATTTTTAGTTTGAGGTATCTTAT (SEQ ID NO: 53) GATCTACAGAGAACTTCTGAGTAAATCTT (SEQ ID NO: 54) SK01_145 chr01 14,462,943 G/A AGTGGTTTAACTCTCCAGGTGTAC (SEQ ID NO: 55) CAGTGGTTTAACTCTCCAGGTGTAT (SEQ ID NO: 56) CGGATAGGGCGGAGAAAGGATAATA (SEQ ID NO: 57) SK01_152 chr01 15,240,761 G/A GAGATACTTGTATATTTATGTTAACACAAGG (SEQ ID NO: 58) GGAGATACTTGTATATTTATGTTAACACAAGA (SEQ ID NO: 59) GCTTTAGTTAAAAATGGGATAGGGGACTT (SEQ ID NO: 60) SK01_160 chr01 15,976,845 C/T CCAGGTCTATTAATTAGGTTCATCTTAC (SEQ ID NO: 61) AACCAGGTCTATTAATTAGGTTCATCTTAT (SEQ ID NO: 62) TATTGATCTCAAAATCCATCTCGATCAGAT (SEQ ID NO: 63) SK01_174 chr01 17,420,652 C/T ATTGCTGCATACGGGAAAGGTTTC (SEQ ID NO: 64) GCTGCATACGGGAAAGGTTTT (SEQ ID NO: 65) CCCAAGTGTCTTGGGACGCCTT (SEQ ID NO: 66) SK01_181 chr01 18,142,949 A/G TCTTAACTACCTGCAAAACGCA (SEQ ID NO: 67) CTTCTTAACTACCTGCAAAACGCG (SEQ ID NO: 68) GTACAAACAAGAGAACCAAGATGGTGTTT (SEQ ID NO: 69) SK01_187 chr01 18,742,940 A/C AAGGTACCAGAATTCTTGCTTGCGA (SEQ ID NO: 70) GGTACCAGAATTCTTGCTTGCGC (SEQ ID NO: 71) GATGGATGTTTTCCGCAAGAATGATTCAA (SEQ ID NO: 72) SK01_194 chr01 19,435,659 A/G ATCAGAGAAAATAGACACTAAGCTGGT (SEQ ID NO: 73) CAGAGAAAATAGACACTAAGCTGGC (SEQ ID NO: 74) GAAATGCTTGTGGAGGTCATCTTGTAATT (SEQ ID NO: 75) SK01_201 chr01 20,094,455 T/A GATTATAAATCATGCATCCTTGTATTTTGATTA (SEQ ID NO: 76) ATAAATCATGCATCCTTGTATTTTGATTT (SEQ ID NO: 77) GTGGCAACAAAGTCGATCTAAAAAAGCTTT (SEQ ID NO: 78) SK02_007 chr02 692,573 C/T GAAATGATCAAATACCCATATTGAGAC (SEQ ID NO: 79) GTGAAATGATCAAAATACCCATATTGAGAT (SEQ ID NO: 80) GGCCAATACAAGCATGATTTTGATGAGAT (SEQ ID NO: 81) SK02_013 chr02 1,331,639 C/G GATCTCCAGGATGGGGGGTTG (SEQ ID NO: 82) GATCTCCAGGATGGGGGTTC (SEQ ID NO: 83) ACTGGAACGATTATTAATGAAGATACGCTT (SEQ ID NO: 84) SK02_022 chr02 2,176,358 T/G GTGGTTAGAAATAAAGGTGCTCCCAT (SEQ ID NO: 85) GGTTAGAAATAAAGGTGCTCCCAG (SEQ ID NO: 86) ATCTCTTGCTTAGAGTAGGTACCCTAAT (SEQ ID NO: 87) SK02_027 chr02 2,670,316 T/G GTATTGGCAGTATATGCCATTGAAGT (SEQ ID NO: 88) GTATTGGCAGTATATGCCATTGAAGG (SEQ ID NO: 89) ACGAGCTTTCTCGACAAATAGTAACTTATTT (SEQ ID NO: 90) SK02_036 chr02 3,646,839 C/T GAAATTATGCAGCAGCATCACATGTC (SEQ ID NO: 91) AGAAATTATGCAGCAGCATCACATGTT (SEQ ID NO: 92) GTGCCTATTTTGTTTATATGAAAGCATTAA (SEQ ID NO: 93) SK02_041 chr02 4,086,381 G/C GTAATTTGAGTAGAATACCAACCATTGG (SEQ ID NO: 94) GTAATTTGAGTAGAATACCAACCATTGC (SEQ ID NO: 95) TTGCCGATGTGGATCAAGTTTGATACTAT (SEQ ID NO: 96) SK02_050 chr02 5,031,650 C/T TTGTGATTAGTTTCTACTTCGACCC (SEQ ID NO: 97) GTTTGTGATTAGTTTCTACTTCGACCT (SEQ ID NO: 98) AAACCTAAACAACAGAAAAAGAGCATGCAA (SEQ ID NO: 99) SK02_059 chr02 5,899,266 G/A CCATTACAAAGTCGTTGCAAAGAC (SEQ ID NO: 100) ACTCCATTACAAAGTCGTTGCAAAGAT (SEQ ID NO: 101) GGCATTTTCTGAACAACCAAATAGCCATT (SEQ ID NO: 102) SK02_070 chr02 7,023,026 C/T AGATTACAAAGCAGAAAACGGAAAGTG (SEQ ID NO: 103) CAAGATTACAAAGCAGAAAAACGGAAAGTA (SEQ ID NO: 104) GCTGATGATGAATGAGTAACGGAAAGATT (SEQ ID NO: 105) SK02_076 chr02 7,569,841 C/G GATTAGAAGTGGTTTGCTCTCCAC (SEQ ID NO: 106) AGAAGTGGTTTGCTCTCCAG (SEQ ID NO: 107) CAGCAAAAAAGATCAGAAATCAGCCTTCAA (SEQ ID NO: 108) SK02_081 chr02 8,097,804 T/C ACAATCAACAACTACTATTAAAGTAAAATAACA (SEQ ID NO: 109) ACAATCAACAACTACTATTAAAGTAAAATAACG (SEQ ID NO: 110) GGCTTTTCGAATCCCACTAACGTGTT (SEQ ID NO: 111) SK02_087 chr02 8,664,091 A/C ATTAAACATCCTGAAATGTAAAATCTAAGATGA (SEQ ID NO: 112) AAACATCCTGAAATGTAAAATCTAAGATGC (SEQ ID NO: 113) TGCATCTGAATGCAAAGGCATGAAAAGAT (SEQ ID NO: 114) SK02_089 chr02 8,892,724 G/A TTTTGTTGTTCTTTGCATTTATTTTTATCCAG (SEQ ID NO: 115) TTTGTTGTTCTTTGCATTTATTTTTATCCAA (SEQ ID NO: 116) ACAAAAATAGAGTAGATGGGAGGTCTGAA (SEQ ID NO: 117) SK02_097 chr02 9,709,344 G/A TCACTTGTAATTTTGTTGATTCCG (SEQ ID NO: 118) CTGCTTCACTTGTAATTTTGTTGATTCCA (SEQ ID NO: 119) GGCATATAAACAACCCAATGTCCTTTCTA (SEQ ID NO: 120) SK02_103 chr02 10,274,577 G/A CAAGTACATCCAACATAATTGGAATGC (SEQ ID NO: 121) CCAAGTACATCCAACATAATTGGAATGT (SEQ ID NO: 122) GTAGAGGGTCAAGTTGATGATAATCATGAA (SEQ ID NO: 123) SK02_110 chr02 10,988,153 C/G CCGTCGACACAATAGTTTCGGG (SEQ ID NO: 124) CCGTCGACACAATAGTTTCGGC (SEQ ID NO: 125) CGTAGCATTCTGCACCAGCCCAA (SEQ ID NO: 126) SK02_117 chr02 11,692,100 A/T GGCATCACAAATCATCGATGTCACAT (SEQ ID NO: 127) GGCATCACAAATCATCGATGTCACAA (SEQ ID NO: 128) TTGAGCTGCCCAGAATCATTAACAAGAAA (SEQ ID NO: 129) SK02_121 chr02 12,137,468 T/C ATGTGTTGGCTTGAAAAGATTAAAAGTCA (SEQ ID NO: 130) GTGTTGGCTTGAAAAGATTAAAAGTCG (SEQ ID NO: 131) CTCCTCTCTTTAGCATCTTTAGTTCTAGTT (SEQ ID NO: 132) SK02_133 chr02 13,259,887 G/C TTTGTTTGAGGACATTGGAGGTG (SEQ ID NO: 133) CTTTTGTTTGAGGACATTGGAGGTC (SEQ ID NO: 134) GGCATTAGCAGCCAATGGGATATTAAATT (SEQ ID NO: 135) SK02_140 chr02 14,015,780 C/T CCATGAATCAAAAGCAACTCGCCG (SEQ ID NO: 136) AACCATGAATCAAAGCAACTCGCCA (SEQ ID NO: 137) AAGATTTAACCAGTTGATGCAAAACTGGAT (SEQ ID NO: 138) SK02_151 chr02 15,098,264 A/G GACTTTTGCATATATGAGAATGTGTCTTT (SEQ ID NO: 139) GACTTTTGCATATATGAGAATGTGTCTTC (SEQ ID NO: 140) GTGGAAGGACATGTTTGCAGTATAGTATA (SEQ ID NO: 141) SK02_160 chr02 15,992,866 T/C ATTCTTGAGGTGGTCCTGCACATAT (SEQ ID NO: 142) CTTGAGGTGGTCCTGCACATAC (SEQ ID NO: 143) TTGAACTTATGGAACAAAGTGCGGACAAA (SEQ ID NO: 144) SK02_167 chr02 16,677,555 A/G CATTTTGTTTTACTACAGTAAATTCCAATAATA (SEQ ID NO: 145) CATTTTGTTTTACTACAGTAAATTCCAATAATG (SEQ ID NO: 146) GGTGTACAAGGAGATGAGGCATCAT (SEQ ID NO: 147) SK02_179 chr02 17,934,730 G/A ATGAATTCACGGAGCCACGCG (SEQ ID NO: 148) CATGAATTCACGGAGCCACGCA (SEQ ID NO: 149) CAGACTTCAGTGATAGGCCTCGTT (SEQ ID NO: 150) SK03_003 chr03 346,941 T/C GTTTTCTGTCTCCGTTACTCCTCTT (SEQ ID NO: 151) TTCTGTCTCCGTTACTCCTCTC (SEQ ID NO: 152) TTGAGCAGGCGAACAAGAGACTGAT (SEQ ID NO: 153) SK03_012 chr03 1,188,148 G/C GGTCTGATCAGAAACCTGTCGAC (SEQ ID NO: 154) GGTCTGATCAGAAACCTGTCGAG (SEQ ID NO: 155) CAGCTTTGGAAACCCATTAAAGATTGCAT (SEQ ID NO: 156) SK03_020 chr03 1,984,702 G/C AAAGATTGCGGTGATGTGCAAAC (SEQ ID NO: 157) CTAAAGATTGCGGTGATGTGCAAAG (SEQ ID NO: 158) CATGAACTGGGAGGTTTCAATCTTTAGTT (SEQ ID NO: 159) SK03_029 chr03 2,859,805 C/T GAGGTAGATTTTATATTTCAGGTAGGG (SEQ ID NO: 160) GGAGGTAGATTTTATATTTCAGGTAGGA (SEQ ID NO: 161) TAGAGCGAAATCCAACCTTATGTTCCAAA (SEQ ID NO: 162) SK03_030 chr03 3,650,141 C/A GGTAAACATGGTCTTTAAGTGGGC (SEQ ID NO: 163) GGTAAACATGGTCTTTAAGTGGGA (SEQ ID NO: 164) TGTCAGGGTTGTCAAAAAACAAGCACAAA (SEQ ID NO: 165) SK03_038 chr03 3,849,579 G/A GAGCCTGGTGTACTAGGTTTTCC (SEQ ID NO: 166) GAGCCTGGTGTACTAGGTTTTCT (SEQ ID NO: 167) CCACTGAAAAATTACTAGGTTAAGCCCAA (SEQ ID NO: 168) SK03_044 chr03 4,380,148 G/C TGTTTTAGGAAGTTGAATTCAAGTTGG (SEQ ID NO: 169) CTTGTTTTAGGAAGTTGAATTCAAGTTGC (SEQ ID NO: 170) CCTTACAAATTTGGTTTTGAAGCTTCAGTT (SEQ ID NO: 171) SK03_050 chr03 5,028,751 T/C AAGATGGGTGAAGGTATTCGACCT (SEQ ID NO: 172) GATGGGTGAAGGTATTCGACCC (SEQ ID NO: 173) GTGTTGCGGAAATGTTTCAAAGGATTGAT (SEQ ID NO: 174) SK03_055 chr03 5,506,616 C/G GTTTCGAAGTGGGCTGTGAG (SEQ ID NO: 175) CTGTTTCGAAGTGGGCTGTGAC (SEQ ID NO: 176) GTAAGTCTTGTGGTATAGTGTTAAGCAGAT (SEQ ID NO: 177) SK03_063 chr03 6,336,483 A/G GCAAGCTCAGTGATGTGACTACAAA (SEQ ID NO: 178) CAAGCTCAGTGATGTGACTACAAG (SEQ ID NO: 179) GAACATTTCATATGCAGGCAGAGTACAAA (SEQ ID NO: 180) SK03_070 chr03 7,004,974 C/T CAGCCATTCTTGTTTTTCTTCTGGC (SEQ ID NO: 181) CAGCCATTCTTGTTTTTCTTCTGGT (SEQ ID NO: 182) TACAATGGTCCCACGAGTATTAATCCAAA (SEQ ID NO: 183) SK03_079 chr03 7,886,609 C/T GTAAATACATGATCAGCCTACTGGG (SEQ ID NO: 184) GGTAAATACATGATCAGCCCTACTGGA (SEQ ID NO: 185) GCCGAAGGCCATATTTCTCAGCATA (SEQ ID NO: 186) SK03_094 chr03 9,360,918 G/A ATAACAGTGATTAAATCCATACCAGATATC (SEQ ID NO: 187) GATAACAGTGATTAAATCCATACCAGATATT (SEQ ID NO: 188) CCCGTCACTTTCTACTACAATGTAATGAT (SEQ ID NO: 189) SK03_098 chr03 9,774,975 C/G TTGCCAAGACAGTTGCGGAC (SEQ ID NO: 190) CTTTGCCAAGACAGTTGCGGAG (SEQ ID NO: 191) GCTCTCGAACAACAGTCTTACTGCAA (SEQ ID NO: 192) SK03_102 chr03 10,199,079 T/C CCCAGAAAGAATTTGCATGCAAATTCAT (SEQ ID NO: 193) CCAGAAAGAATTTGCATGCAAATTCAC (SEQ ID NO: 194) AATGTAAGCATACTACTCATCCTGGTCTT (SEQ ID NO: 195) SK03_108 chr03 10,764,491 C/CT GGATGAAATATAGGAAGATGACCTTTG (SEQ ID NO: 196) GGGATGAAATATAGGAAGATGACCTTTT (SEQ ID NO: 197) GACGGACCCAACTAATTTACATGACAATT (SEQ ID NO: 198) SK03_116 chr03 11,581,327 C/A AGATCTAAAGTAACTTCTAAGTAAAAAATTGAG (SEQ ID NO: 199) AGATCTAAAGTAACTTCTAAGTAAAAAATTGAT (SEQ ID NO: 200) GAGTATTCTGGGTCAAACACTTGGATATT (SEQ ID NO: 201) SK03_120 chr03 11,967,930 T/C ATCCGAAGATGAACTGATGATGAGAT (SEQ ID NO: 202) CCGAAGATGAACTGATGATGAGAC (SEQ ID NO: 203) GTGATGTGTGCCGGCAATCATCAT (SEQ ID NO: 204) SK03_126 chr03 12,648,089 C/G AGTTTCTCTCAGAGACCATGTTTGG (SEQ ID NO: 205) AGTTTCTCTCAGAGACCATGTTTGC (SEQ ID NO: 206) ACTCACAAACCAAGGAAAGTGAGATCAAA (SEQ ID NO: 207) SK03_141 chr03 14,091,613 T/A ATTTTTCTGGAACCAGAGAAGACTGTA (SEQ ID NO: 208) TTTCTGGAACCAGAGAAGACTGTT (SEQ ID NO: 209) CATGTCGTGTGTTTGTGTAAGTGTAACTT (SEQ ID NO: 210) SK03_147 chr03 14,663,292 T/G ATTAAAAAATCATTTGCCGCAACTAAGAGT (SEQ ID NO: 211) AAAAAATCATTTGCCGCAACTAAGAGG (SEQ ID NO: 212) GCCGTGGTTTTAATGAGAATGGCTAAAAT (SEQ ID NO: 213) SK03_151 chr03 15,086,146 G/A GAAAGTGTATTAACAAACCAGAAATAGCC (SEQ ID NO: 214) AGAAAGTGTATTAACAAACCAGAAATAGCT (SEQ ID NO: 215) GCGCTTGCATGATATTAAGTGATGCAATT (SEQ ID NO: 216) SK03_158 chr03 15,836,356 T/C ATATAAGTTATAGCGTTTTATACAATGTAAT (SEQ ID NO: 217) CTATATAAGTTATAGCGTTTTATACAATGTAAC (SEQ ID NO: 218) TGTACGATTGAAGGGTGCAAAGTGTTATT (SEQ ID NO: 219) SK03_166 chr03 16,576,907 C/G ACACTAGATAATTGGACGGTATTTCAC (SEQ ID NO: 220) ACACTAGATAATTGGACGGTATTTCAG (SEQ ID NO: 221) ACATGGCAGTCCCATTATGAATAACCAAA (SEQ ID NO: 222) SK03_173 chr03 17,294,459 A/G CTTACTTTGGGGCAGGTGCA (SEQ ID NO: 223) CTTACTTTGGGGCAGGTGCG (SEQ ID NO: 224) AAAGGCGGAAAATCCATTGATCATGAGAA (SEQ ID NO: 225) SK03_180 chr03 18,029,963 A/G GTAATTAATTTATATCTTTTGTTGTTACTTTTA (SEQ ID NO: 226) GTAATTAATTTATATCTTTTGTTGTTACTTTTG (SEQ ID NO: 227) GCATAGAATGCCATAAAATAAAAAGTTCAA (SEQ ID NO: 228) SK03_191 chr03 19,147,453 G/A GATGTATATGTTAGTAATACACATTTATTTAC (SEQ ID NO: 229) GATGTATATGTTAGTAATACACATTTATTTAT (SEQ ID NO: 230) GTAGAATGTATGTGTCCATTATGTGGCTT (SEQ ID NO: 231) SK03_203 chr03 20,305,999 T/A CCTTATCACCACCCCCTGAA (SEQ ID NO: 232) CCTTATCACCACCCCCTGGAT (SEQ ID NO: 233) GTCGGAGAGGCAGCTGCTGATT (SEQ ID NO: 234) SK03_219 chr03 21,856,420 C/A AAATCATTATTTTTATTTATGTACACACATTG (SEQ ID NO: 235) AAATCATTATTTTTATTTATGTACACACATTT (SEQ ID NO: 236) TTTAGTTTTTATTAGTGATGGCGGCACATT (SEQ ID NO: 237) SK03_226 chr03 22,613,501 T/G GACATTTAAACCAAATATGTCAATTGTATTTA (SEQ ID NO: 238) GACATTTAAACCAAATATGTCAATTGTATTTC (SEQ ID NO: 239) CTGACGTAGCACGAGGAACATAAGAA (SEQ ID NO: 240) SK03_237 chr03 23,698,224 A/T GATTTGGCGAAGTGAAGTGCGAAA (SEQ ID NO: 241) TGGCGAAGTGAAGTGCGAAT (SEQ ID NO: 242) GTCGCCCTGTTCATTCACCTCAATT (SEQ ID NO: 243) SK03_245 chr03 24,520,054 G/A CGTTCACAATTATATGGAGAATACCAAC (SEQ ID NO: 244) CCGTTCACAATTATATGGAGAATACCAAT (SEQ ID NO: 245) TCGAATGTCGTGATATGATAATATTTCTTT (SEQ ID NO: 246) SK03_252 chr03 25,218,994 A/C AACTCCGCCATAGGCTCCATCT (SEQ ID NO: 247) CTCCGCCATAGGCTCCATCG (SEQ ID NO: 248) TGGCCTCGCAATTCCAGGAAAGTTA (SEQ ID NO: 249) SK03_258 chr03 25,847,016 A/T CATATTTATTAGCATTTTTTGTTCTCTAACA (SEQ ID NO: 250) CTCATATTTATTAGCATTTTTTGTTCTCTAACT (SEQ ID NO: 251) ATTTGCATGACGAAAATGCCACCTTAGAA (SEQ ID NO: 252) SK04_008 chr04 761,034 A/G AAATATGACAGACTGCTGCCGT (SEQ ID NO: 253) AAATATGACAGACTGCTGCCGC (SEQ ID NO: 254) CCATCTCATGGACCGTGTTTTCCAT (SEQ ID NO: 255) SK04_015 chr04 1,489,102 T/A AGGATGGAGATCCGTGCCTATTTAA (SEQ ID NO: 256) AGGATGGAGATCCGTGCCTATTTAT (SEQ ID NO: 257) CCCATGTGCAATGAATATATTCGAACGAT (SEQ ID NO: 258) SK04_023 chr04 2,268,501 C/T GGCAAGTGCAGCCTCTCCG (SEQ ID NO: 259) GTGGCAAGTGCAGCCCTCTCCA (SEQ ID NO: 260) AGTGGCAGAGTACTTGAATTGTCACTAAA (SEQ ID NO: 261) SK04_029 chr04 2,861,681 A/G CCAAGAAAGAATTAGGAGAGGAAGAT (SEQ ID NO: 262) CCAAGAAAGAATTAGGAGAGGAAGAC (SEQ ID NO: 263) ATGTGCTTCCCAATTTGTTGAAGTTGGAT (SEQ ID NO: 264) SK04_045 chr04 4,461,865 A/G CCATTCTTGAGACCATGAATGAGTAT (SEQ ID NO: 265) CCATTCTTGAGACCATGAATGAGTAC (SEQ ID NO: 266) ACAGGACCTCAAAATTGTCATGATGGTTA (SEQ ID NO: 267) SK04_051 chr04 5,145,335 C/T GTCGGCCCTCCACTCACC (SEQ ID NO: 268) GTCGGCCCTCCACTCACT (SEQ ID NO: 269) GACGAAGAGGAAATGAACACCATAAAAGAT (SEQ ID NO: 270) SK04_060 chr04 5,981,604 C/T GTAAAGATGCTGGTGAAAGGAGG (SEQ ID NO: 271) GGTAAAGATGCTGGTGAAAGGAGA (SEQ ID NO: 272) CGGCGAACATCCACCTTCTTAAAGAA (SEQ ID NO: 273) SK04_069 chr04 6,881,107 G/T ATCTGTAGTCATTGGCGTCTGAG (SEQ ID NO: 274) CATCTGTAGTCATTGGCGTCTGAT (SEQ ID NO: 275) CAAGACGGGGATGAATTGGTAGTCTA (SEQ ID NO: 276) SK04_080 chr04 7,963,761 T/C GTAGTACTAATTAAAATTCTACCCACAGATT (SEQ ID NO: 277) AGTACTAATTAAAATTCTACCCACAGATC (SEQ ID NO: 278) TTCCACATTTCTTATTGTTCACTTTCCCAT (SEQ ID NO: 279) SK04_090 chr04 9,031,906 G/T TCAGACTGTGATCAGCATCTTTCG (SEQ ID NO: 280) GTTCAGACTGTGATCAGCATCTTTCT (SEQ ID NO: 281) CAGCTGATCAGCAGTTTCTTATCTATGAT (SEQ ID NO: 282) SK04_098 chr04 9,841,138 T/C GATAAGGAGGTAATATTGCATGCTATCT (SEQ ID NO: 283) ATAAGGAGGTAATATTGCATGCTATCC (SEQ ID NO: 284) GAGTTCAAGAAAACAAGAAACTCTGAAGTA (SEQ ID NO: 285) SK04_107 chr04 10,709,483 C/T TGTTATCATGTCCAACTAAATAAATAAAATCC (SEQ ID NO: 286) GTTATCATGTCCAACTAAATAAATAAAATCT (SEQ ID NO: 287) GGCTCCGTTTATTTTGTGAAATGTCATTAA (SEQ ID NO: 288) SK04_114 chr04 11,406,173 C/T CATGGTCTTGCGCGAAGTACTG (SEQ ID NO: 289) GCATGGTCTTGCGCGAAGTACTA (SEQ ID NO: 290) GCCTTTCTCACAAGAGACTTCAGCAT (SEQ ID NO: 291) SK04_122 chr04 12,170,583 G/C CATTGTATTATGTCGGCCTGCATAC (SEQ ID NO: 292) CATTGTATTATGTCGGCCTGCATAG (SEQ ID NO: 293) CGCCAGATAAAGATGCCTCTCCATA (SEQ ID NO: 294) SK04_129 chr04 12,856,335 C/G AAAAGAAAACAGACATGCCACTTGAAC (SEQ ID NO: 295) AAAAGAAAACAGACATGCCACTTGAAG (SEQ ID NO: 296) GGCTATCATATACTCACCTTTTCGGTTAA (SEQ ID NO: 297) SK04_138 chr04 13,788,710 T/A GATAACATGTTCGAAAATGAACATAGTATAAA (SEQ ID NO: 298) GATAACATGTTCGAAAATGAACATAGTATAAT (SEQ ID NO: 299) TCCCACAAAAAGTAGGCATCATGACTTAT (SEQ ID NO: 300) SK04_143 chr04 14,286,771 C/A TGCGTTCTCACCTACTCTCCG (SEQ ID NO: 301) CTTGCGTTCTCACCTACTCTCCT (SEQ ID NO: 302) GTTCCACTTGACGGAATTCACACGTA (SEQ ID NO: 303) SK04_149 chr04 14,890,467 G/A CCAATACTAGCCCTACTAATTTTAACTG (SEQ ID NO: 304) CCAATACTAGCCCTACTAATTTTAACTA (SEQ ID NO: 305) AGTTGGCTGCATTAATGCAGTAACTGTT (SEQ ID NO: 306) SK04_158 chr04 15,783,359 T/C ACTGTTAACTATTCTGCATGGGTTTCT (SEQ ID NO: 307) CTGTTAACTATTCTGCATGGGTTTCC (SEQ ID NO: 308) CACCCCGTCTTGTGTTGGTTTAGAA (SEQ ID NO: 309) SK04_164 chr04 16,411,742 T/G GCAATTAAGAGATCCTGCAAACCAAT (SEQ ID NO: 310) GCAATTAAGAGATCCTGCAAACCAAG (SEQ ID NO: 311) CTGAGTACCATTACTGCTAGTTTCTTCTT (SEQ ID NO: 312) SK04_174 chr04 17,361,613 A/G ATTATACATATACCATGTTGACTTCAAAATTAT (SEQ ID NO: 313) ATACATATACCATGTTGACTTCAAAATTAC (SEQ ID NO: 314) AAATGTGGTTTCGTCTATTTATGGTGAGTA (SEQ ID NO: 315) SK04_180 chr04 17,980,044 G/T AAAAGTTTCAACTAAAAATGAGTTCCTAGC (SEQ ID NO: 316) AAAAAAAGTTTCAACTAAAAATGAGTTCCTAGA (SEQ ID NO: 317) CTGGTCTGAGGTATGCCTATTACCAA (SEQ ID NO: 318) SK04_183 chr04 18,286,839 C/T CTTGCAGGTATCGCTAATATGACC (SEQ ID NO: 319) CTCTTGCAGGTATCGCTAATATGACT (SEQ ID NO: 320) GCGCGAAGAAATCAATAGGAGGACAA (SEQ ID NO: 321) SK04_189 chr04 18,867,610 A/G CCAGCCATCCAGAACAGTCTCA (SEQ ID NO: 322) CAGCCATCCAGAACAGTCTCG (SEQ ID NO: 323) GTTATGTGGGACTCCGAAGTCTTCAA (SEQ ID NO: 324) SK04_195 chr04 19,452,120 A/C ATTATTTATTTTCACATATCATGAATTAATCA (SEQ ID NO: 325) ATTATTTATTTTCACATATCATGAATTAATCC (SEQ ID NO: 326) GAGCCCAATAAGACAACTGGCTCAA (SEQ ID NO: 327) SK04_200 chr04 19,985,006 C/T AGAGCAAGAGATGGATTTGTGGAC (SEQ ID NO: 328) GAGAGCAAGAGATGGATTTGTGGAT (SEQ ID NO: 329) CTCAAAAGCATCCTTTCACATACAGCAAA (SEQ ID NO: 330) SK04_204 chr04 20,421,551 C/T ACGTGAAACTGAAGAAATTAAGGCG (SEQ ID NO: 331) CTACGTGAAACTGAAGAAATTAAGGCA (SEQ ID NO: 332) GGCTGTGAATGAGAAATCTGAGCCTT (SEQ ID NO: 333) SK05_003 chr05 334,140 T/C GAATGCTTTTGTAAGAAGACCGTGGT (SEQ ID NO: 334) AATGCTTTTGTAAGAAGACCGTGGC (SEQ ID NO: 335) GAAGACTCAAATCTGTGGGCAGGAA (SEQ ID NO: 336) SK05_010 chr05 965,393 G/A ACTGTGTTTCATTGACGGAACTTCC (SEQ ID NO: 337) AAAACTGTGTTTCATTGACGGAACTTCT (SEQ ID NO: 338) GAAGTGTGGATCATTAGCGTTCGGTT (SEQ ID NO: 339) SK05_015 chr05 1,482,793 C/G TGGTAAACTTTCGAGTTCTGTTATATTTG (SEQ ID NO: 340) GGTAAACTTTCGAGTTCTGTTATATTTC (SEQ ID NO: 341) GAGTCTAGCTCGAGCAGTATAATACTTT (SEQ ID NO: 342) SK05_017 chr05 1,738,443 T/A GGTCTAAGTAGTTGCTAGTCTGCTA (SEQ ID NO: 343) GGTCTAAGTAGTTGCTAGTCTGCTT (SEQ ID NO: 344) GTGGGAACATAAAGACCATAGGCTCAT (SEQ ID NO: 345) SK05_032 chr05 3,176,475 C/A TCTTTCTGAAGAGTAAGCAATGCATC (SEQ ID NO: 346) ATATTCTTTCTGAAGAGTAAGCAATGCATA (SEQ ID NO: 347) GCTCGCCAGAAGACAGGAAATACTA (SEQ ID NO: 348) SK05_036 chr05 3,586,025 T/C ATCTACAATGGCGAACGATGCTGAT (SEQ ID NO: 349) CTACAATGGCGAACGATGCTGAC (SEQ ID NO: 350) CAGAAGATCAGTCCATCCGCTCAT (SEQ ID NO: 351) SK05_039 chr05 3,920,778 T/C AAGGAAAGAAACATTGCACTAGCAAAA (SEQ ID NO: 352) AAGGAAAGAACATTGCACTAGCAAAG (SEQ ID NO: 353) CACTGTATTACGTTCTATGTCGAGCTAT (SEQ ID NO: 354) SK05_059 chr05 5,867,130 C/T ACAGCTCCATTTGGATGATTCGG (SEQ ID NO: 355) AGACAGCTCCATTTGGATGATTCGA (SEQ ID NO: 356) CGCCAACCCCATTAGCCTCCTT (SEQ ID NO: 357) SK05_068 chr05 6,802,254 C/T AACATTACATGCCAAAATTTGTGCG (SEQ ID NO: 358) CCTAACATTACATGCCAAATTTGTGCA (SEQ ID NO: 359) GTCAAATTGGATTATTACTACCCAACACAA (SEQ ID NO: 360) SK05_075 chr05 7,508,802 C/T GGCATATTTTAAATGAAAATGGATCTGATTG (SEQ ID NO: 361) AGGCATATTTTAAATGAAAATGGATCTGATTA (SEQ ID NO: 362) AAACTCAGCAATTCTACGAGGACATTCTT (SEQ ID NO: 363) SK05_087 chr05 8,728,830 G/C CGAGACAATTCTATGTATTTAATTATTTATGG (SEQ ID NO: 364) CGAGACAATTCTATGTATTTAATTATTTATGC (SEQ ID NO: 365) CCTAATTCACATTTCTTATGATGGGGTCTT (SEQ ID NO: 366) SK05_092 chr05 9,206,921 A/T AACAATTTCCTCGGTTTATTCCATTATGTA (SEQ ID NO: 367) AACAATTTCCTCGGTTTATTCCATTATGTT (SEQ ID NO: 368) GTGTTTGTCTGAAACCCCATTTCATAGAT (SEQ ID NO: 369) SK05_100 chr05 9,957,378 G/A AAAGAAAATTTTTCATGAACGTAATTTCACTC (SEQ ID NO: 370) AAAGAAAATTTTTCATGAACGTAATTTCACTT (SEQ ID NO: 371) GATTCTATTCCCATAATCGAAAATTAGAAA (SEQ ID NO: 372) SK05_108 chr05 10,770,429 G/A AACAAATGATATCAGAGTTGACCACC (SEQ ID NO: 373) GTAACAAATGATATCAGAGTTGACCACT (SEQ ID NO: 374) CATATCACTTAGTTCCCTACTCTGCTT (SEQ ID NO: 375) SK05_112 chr05 11,181,683 C/T GTCAAATAGTCCTTAGGATTTGCAAC (SEQ ID NO: 376) AATGTCAAATAGTCCTTAGGATTTGCAAT (SEQ ID NO: 377) TAGTTGAAGCCTTAAAGCAAAGGGAATA (SEQ ID NO: 378) SK05_115 chr05 11,522,463 G/A AGTCGGGTTCGTCCCCG (SEQ ID NO: 379) AAAACTAGTCGGGTTCGTCCCCA (SEQ ID NO: 380) CCAATTAACTTAAGCATCAGAGGGTCATT (SEQ ID NO: 381) SK05_122 chr05 12,226,877 T/C AAGGCATTAACATACTATCTCAACTTTAATAA (SEQ ID NO: 382) GGCATTAACATACTATCTCAACTTTAATAG (SEQ ID NO: 383) GTGATAACGTGGAACTTAATTTACCTTAAT (SEQ ID NO: 384) SK05_132 chr05 13,197,953 A/G CAATAGTTGGTAAATAAATTCACGAGTTTCT (SEQ ID NO: 385) AATAGTTGGTAAATAAATTCACGAGTTTCC (SEQ ID NO: 386) CTTGGGTGGTGAAGGAAGAGGTTT (SEQ ID NO: 387) SK05_137 chr05 13,669,738 A/G ACAGACCTTCATCGTCCATTAGGT (SEQ ID NO: 388) CAGACCTTCATCGTCCATTAGGC (SEQ ID NO: 389) ATAACTGCACGGACAGGCAAACCAT (SEQ ID NO: 390) SK05_148 chr05 14,783,047 C/T ATTCATCATATAAAAGAGCAAAAAACTGCG (SEQ ID NO: 391) CATTCATCATATAAAAGAGCAAAAAACTGCA (SEQ ID NO: 392) CAAACTGACAGACACCAAATGAAGCAAAT (SEQ ID NO: 393) SK05_155 chr05 15,509,349 C/G ACAGGCAGTGGACCTGCCG (SEQ ID NO: 394) ACAGGCAGTGGACCTGCCC (SEQ ID NO: 395) CTCACCACAGACCGGACCCATT (SEQ ID NO: 396) SK05_162 chr05 16,213,713 A/G AAATATTTCTATTTAGCATGCTTGTTGAAAT (SEQ ID NO: 397) AAATATTTCTATTTAGCATGCTTGTTGAAAC (SEQ ID NO: 398) GAACTCCGTTAGCTGAACTCCACAT (SEQ ID NO: 399) SK06_006 chr06 595,629 G/T CCCGTACCATCAAAATTAAATTATTATTGTTC (SEQ ID NO: 400) CCCGTACCATCAAAATTAAATTATTATTGTTA (SEQ ID NO: 401) CGTGCTAATTTTATCCATAATGTCAACCAA (SEQ ID NO: 402) SK06_013 chr06 1,319,683 T/A AACTCATTTAAACAAGATAGTCTTAGGCA (SEQ ID NO: 403) AACTCATTTAAACAAGATAGTCTTAGGCT (SEQ ID NO: 404) CTTTAATCTTTCTTTTTTGCTCACGGCTAA (SEQ ID NO: 405) SK06_022 chr06 2,212,622 C/T TGTGAGCGCTAAATTTATTGTATCTG (SEQ ID NO: 406) AACTTGTGAGCGCTAAATTTATTGTATCTA (SEQ ID NO: 407) CCCAATACTTTTATTTGTGATAACTTATTT (SEQ ID NO: 408) SK06_029 chr06 2,922,645 T/G CACTCATAAGTCAAAACCCACTCA (SEQ ID NO: 409) CACTCATAAGTCAAAACCCACTCC (SEQ ID NO: 410) GTCTCCTTTAGCCCCAAACTCCATT (SEQ ID NO: 411) SK06_035 chr06 3,466,107 G/GCAAT ATACCAAATATTCTATTCTGATACTAACAATAC (SEQ ID NO: 412) ATACCAAATATTCTATTCTGATACTAACAATAA (SEQ ID NO: 413) CCAAGACCAAGCATTGATTTGATGCTATA (SEQ ID NO: 414) SK06_042 chr06 4,158,730 G/A CATAACTATCTCGTGAAGTGGCG (SEQ ID NO: 415) CCATAACTATCTCGTGAAGTGGCA (SEQ ID NO: 416) CCTGGTTTGAGACACTAAGGAAACAAATA (SEQ ID NO: 417) SK06_048 chr06 4,762,626 A/G ATTATTCCATCAATCCAGCATATGTTCTT (SEQ ID NO: 418) ATTCCATCAATCCAGCATATGTTCTC (SEQ ID NO: 419) GTACGTTAACTGAGTATCAAAGGGAGAAA (SEQ ID NO: 420) SK06_056 chr06 5,568,466 G/A GTTTGTAGCACCACTTCGACCG (SEQ ID NO: 421) ATGTTTGTAGCACCACTTCGACCA (SEQ ID NO: 422) GGGCTGCTGCCTGGGGATTAAT (SEQ ID NO: 423) SK06_063 chr06 6,252,397 T/C AAAATGTTAGAGGAAAAATTGCAGTTTTATTCA (SEQ ID NO: 424) ATGTTAGAGGAAAAATTGCAGTTTTATTCG (SEQ ID NO: 425) GAATAAAAAATGCCACCCTCTCTAAGTTAA (SEQ ID NO: 426) SK06_067 chr06 6,735,278 C/T CACATGTTTTTAGTTAAGACGAGAAAGTC (SEQ ID NO: 427) CACATGTTTTTAGTTAAGACGAGAAAGTT (SEQ ID NO: 428) CTAGAGACGAAGTGAGTGGCTGTTT (SEQ ID NO: 429) SK06_075 chr06 7,481,376 A/G GAACCTCTCCAAAATCAGACAAGGA (SEQ ID NO: 430) AACCTCTCCAAAATCAGACAAGGG (SEQ ID NO: 431) TCTTCACTTTCTAAATCTTCCTAGCAGTTT (SEQ ID NO: 432) SK06_081 chr06 8,116,800 C/T CAATAAAGGTTGTGCCAATAATTCCG (SEQ ID NO: 433) CACAATAAAGGTTTGTGCCAATAATTCCA (SEQ ID NO: 434) TGTGGATGGTTGAAGAGAGGACCAA (SEQ ID NO: 435) SK06_098 chr06 9,825,085 A/G ATGTCTCATTAGCTGCCTGTGTAGT (SEQ ID NO: 436) GTCTCATTAGCTGCCTGTGTAGC (SEQ ID NO: 437) CCACAGACTCCAAATGATGCTGGAA (SEQ ID NO: 438) SK06_105 chr06 10,529,367 G/A TCTCTCAGATGATTGTTCCTTTGTAC (SEQ ID NO: 439) ATATTCTCTCAGATGATTGTTCCTTTGTAT (SEQ ID NO: 440) CGATGGTGTTTATGCTAAAACTTGCACAA (SEQ ID NO: 441) SK06_113 chr06 11,333,635 C/G ATAATAAAGACACTATGGTTACATGTTGTG (SEQ ID NO: 442) ATAATAAAGACACTATGGTTACATGTTGTC (SEQ ID NO: 443) CTATATCACCATTAAATGTCTAGGAACTTT (SEQ ID NO: 444) SK06_121 chr06 12,126,865 C/T ATGTATGATAATTTTGCTTGTGTACCTTC (SEQ ID NO: 445) CATGTATGATAATTTTGCTTGTGTACCTTT (SEQ ID NO: 446) CAAACTACCATGCTCAACAATTTCTCCAT (SEQ ID NO: 447) SK06_129 chr06 12,884,681 G/A CATTCATGAATAATATCATCTACGAGTCG (SEQ ID NO: 448) CATTCATGAATAATATCATCTACGAGTCA (SEQ ID NO: 449) CAGTGATCAACCTGCCAGGTACAAA (SEQ ID NO: 450) SK06_136 chr06 13,589,744 C/T CTTGGAACTTATAACTGGAATGGAAG (SEQ ID NO: 451) GTCTTGGAACTTATAACTGGAATGGAAA (SEQ ID NO: 452) TCTCCCCGCTCGACGGGTTAAA (SEQ ID NO: 453) SK06_139 chr06 13,939,397 T/C CCAATAGATCATTCATATATGCAAAGGCT (SEQ ID NO: 454) CAATAGATCATTCATATATGCAAAGGCC (SEQ ID NO: 455) CTGCTGTATGGGTGAAGTCTCTCTT (SEQ ID NO: 456) SK06_145 chr06 14,458,496 G/A CACCACACTCCTCTCAAGTTCC (SEQ ID NO: 457) CCACCACACTCCTCTCAAGTTCT (SEQ ID NO: 458) GAGTTGGGTGAGAGGGCTACGAT (SEQ ID NO: 459) SK06_152 chr06 15,217,474 G/A TCTTCTTCTTCTTGCTCTGCCC (SEQ ID NO: 460) CTTCTTCTTCTTCTTGCTCTGCCT (SEQ ID NO: 461) GGATCCTTCCCTTCTCTTTGATATTGAT (SEQ ID NO: 462) SK06_157 chr06 15,748,972 T/C CGGATTGATCACGTGGAATTCAGAT (SEQ ID NO: 463) GATCACGTGGAATTCAGAC (SEQ ID NO: 464) AAGAGGCCCAAATCAAGTTGAAGTTGTAT (SEQ ID NO: 465) SK06_162 chr06 16,161,699 T/G GCAGTATTACCATGTGAGGGTCT (SEQ ID NO: 466) GCAGTATTACCATGTGAGGGTCG (SEQ ID NO: 467) CTCCACTAAAAAGCCTATTGACAATGGTT (SEQ ID NO: 468) SK06_170 chr06 16,992,420 C/T AACGACAGCGGGAATTTGTCCC (SEQ ID NO: 469) GAACGACAGCGGGAATTTGTCCT (SEQ ID NO: 470) CTTCGGATACTTGGGCATTGAAGGAT (SEQ ID NO: 471) SK06_177 chr06 17,716,603 /C CATTAGTAGACCGTGATCACAGACA (SEQ ID NO: 472) AGTAGACCGTGATCACAGACC (SEQ ID NO: 473) TGCAAGGTTTCATGACATTTCAGTTGGAA (SEQ ID NO: 474) SK06_183 chr06 18,329,762 A/C ACACTTCTAAGGTAATACTAACAAACTAATATA (SEQ ID NO: 475) CACTTCTAAGGTAATACTAACAAACTAATATC (SEQ ID NO: 476) TGCGTTGTTAGTTCTGTCAAGCATAAGAA (SEQ ID NO: 477) SK06_191 chr06 19,062,430 T/A AAATTCAAGAGCTGAATCGTAGAACCA (SEQ ID NO: 478) AAATTCAAGAGCTGAATCGTAGAACCT (SEQ ID NO: 479) CGGACCTTAATTTCATGGAGGTGTGTA (SEQ ID NO: 480) SK06_190 chr06 19,441,863 T/G GATATAACTAACAAAGTATCCTTTTTGATCTA (SEQ ID NO: 481) GATATAACTAACAAAGTATCCTTTTTGATCTC (SEQ ID NO: 482) CCATTAATCCAAACCATATGTTACTGTGTT (SEQ ID NO: 483) SK06_198 chr06 19,832,793 A/T AAGAAATATTGGAAAAAGCCATGGAGTTA (SEQ ID NO: 484) AAGAAATATTGGAAAAAGCCATGGAGTTT (SEQ ID NO: 485) GAAGTGAGCGTTGGAGGTGTGTTTT (SEQ ID NO: 486) SK06_205 chr06 20,519,401 A/C AGATGAGGGACATTGTTGAAAGTTATATT (SEQ ID NO: 487) GATGAGGGACATTGTTGAAAGTTATATG (SEQ ID NO: 488) GGATTTGAATCAACATGCTAAAATCAGCAA (SEQ ID NO: 489) SK06_212 chr06 21,163,622 C/T GAAAACGGTTCTTTTCTTACCCTGC (SEQ ID NO: 490) AAGAAAACGGTTCTTTTCTTACCCTGT (SEQ ID NO: 491) CAAATTAGAAGAGGGAAAATTGGGGGTT (SEQ ID NO: 492) SK06_222 chr06 22,221,399 A/G TATCAGCAAAAAGTGTAAAGAAAAAAACAGAA (SEQ ID NO: 493) CAGCAAAAAGTGTAAAGAAAAAAAACAGAG (SEQ ID NO: 494) GGGTAGGACTTTGGTTGGTTGTACAA (SEQ ID NO: 495) SK06_235 chr06 23,505,587 T/C GAGCACTGGGCGCCTATCT (SEQ ID NO: 496) GAGCACTGGGCGCCTATCC (SEQ ID NO: 497) AGAAGCCCTGTGCGCAGTCCTA (SEQ ID NO: 498) SK06_240 chr06 23,965,800 AT/A ACCTTTATTTTATATGTGTTTCTATTGTTTTAT (SEQ ID NO: 499) ACCTTTATTTTATATGTGTTTCTATTGTTTTAA (SEQ ID NO: 500) CGCCCCCGATACTCTTTTTCTTTTATTTT (SEQ ID NO: 501) SK06_249 chr06 24,893,349 T/C CGCGACGCCGCCTGTATTGA (SEQ ID NO: 502) GCGACGCCGCCTGTATTGG (SEQ ID NO: 503) ATTTATTCATTACCGTCGGCAGGCAATTT (SEQ ID NO: 504) SK06_256 chr06 25,631,452 G/T AGTCTCGAAGAGATGAAGATTATTGAC (SEQ ID NO: 505) ATAAGTCTCGAAGAGATGAAGATTATTGAA (SEQ ID NO: 506) GGCCATCTCTACGTATAGACATGTATATT (SEQ ID NO: 507) SK07_006 chr07 629,335 G/T GAGATGTGCAGTTGTTGCAGCAG (SEQ ID NO: 508) GAGATGTGCAGTTGTTGCAGCAT (SEQ ID NO: 509) GAACCTGGAAACCTTTGTTTGTATCCAT (SEQ ID NO: 510) SK07_022 chr07 2,223,190 A/G ATACAATTATTCTTACTTTCTTTCAAGTTTTT (SEQ ID NO: 511) ATACAATTATTCTTACTTTCTTTCAAGTTTTC (SEQ ID NO: 512) CAGAGGTTCGCACTCCAATACTTAAATTA (SEQ ID NO: 513) SK07_043 chr07 4,337,321 /T CCTCCTTGTTTTCTTTCCTTTTTTC (SEQ ID NO: 514) CCTCCTCCTTGTTTTCTTTCCTTTTTTT (SEQ ID NO: 515) CGGATCCAAACGAAAAGGACAAGAATTA (SEQ ID NO: 516) SK07_047 chr07 4,726,387 C/T GAACTTTACTTTTGTTTCTATAGATAAGCAG (SEQ ID NO: 517) GGAACTTTACTTTTGTTTCTATAGATAAGCAA (SEQ ID NO: 518) AGGAACGAAAAGGGAAAGAACACATGTT (SEQ ID NO: 519) SK07_055 chr07 5,540,765 G/A TCTTTAACGTAACCACTTGCTCG (SEQ ID NO: 520) CTTCTTTAACGTAACCACTTGCTCA (SEQ ID NO: 521) GAATGACACTCGTATCCAACAGTCTATTT (SEQ ID NO: 522) SK07_064 chr07 6,363,895 G/T TTACGTTTGTCCAACAACTAGCCC (SEQ ID NO: 523) TTACGTTTGTCCAACAACTAGCCA (SEQ ID NO: 524) GGGCATTTTGCTCATTTCACCACAAAATA (SEQ ID NO: 525) SK07_073 chr07 7,277,884 A/T TTGGCTGGTTAACCTGTTCATCAAA (SEQ ID NO: 526) TGGCTGGTTAACCTGTTCATCAAT (SEQ ID NO: 527) CAGTTCACATGGCCAAACCTGGAAA (SEQ ID NO: 528) SK07_079 chr07 7,947,552 A/T GGAATATCATGTTGCATCAATATCACCAT (SEQ ID NO: 529) GGAATATCATGTTGCATCAATATCACCAA (SEQ ID NO: 530) GTAGTTTGATCAATTTCTTCTGCAGCAGAT (SEQ ID NO: 531) SK07_083 chr07 8,311,766 G/C AGTATTATAAGGACCATATCAATTTTCTGG (SEQ ID NO: 532) AGTATTATAAGGACCATATCAATTTTCTGC (SEQ ID NO: 533) GAAGACATGAAGTCCTATCAATATGCTATT (SEQ ID NO: 534) SK07_094 chr07 9,425,380 G/A CGAGAAACTTCTACACTTGGATTCG (SEQ ID NO: 535) AACGAGAAACTTCTACACTTGGATTCA (SEQ ID NO: 536) CACTAATAATTCTCTACCTAACTTCCTCAA (SEQ ID NO: 537) SK07_100 chr07 9,959,902 G/A AAACTTCGAGGGTGCTAAATTTAATTTTTC (SEQ ID NO: 538) CAAACTTCGAGGGTGCTAAATTTAAATTTTTT (SEQ ID NO: 539) CTCGTTTCCTCCGAGATTTGTTGTAATTA (SEQ ID NO: 540) SK07_106 chr07 10,609,211 G/A GTATATGTTATAATTGTAATGCTGTGGAG (SEQ ID NO: 541) CTTGTATATGTTATAATTGTAATGCTGTGGAA (SEQ ID NO: 542) CCATGTAATCCACAAATATGAGATAAGGTA (SEQ ID NO: 543) SK07_114 chr07 11,374,555 G/A AATCTATGTTTGTTTTTTGATTGGTCACG (SEQ ID NO: 544) ATAATCTATGTTTGTTTTTTGATTGGTCACA (SEQ ID NO: 545) CAAAGTCGGACTTGTGCTTCCGAAT (SEQ ID NO: 546) SK07_126 chr07 12,578,781 G/A ATAAGTCCCCAGATGGTGGTAAATG (SEQ ID NO: 547) CATAAGTCCCCAGATGGTGGTAAATA (SEQ ID NO: 548) CGTCTGGTCGAGATGGGCAAATATA (SEQ ID NO: 549) SK07_133 chr07 13,311,218 T/C GTGGATTCTGATTATTGGTTTTACGAATTTA (SEQ ID NO: 550) GGATTCTGATTATTGGGTTTTACGAATTTG (SEQ ID NO: 551) GGGGCCATGTATAAAGGAGTTCCTA (SEQ ID NO: 552) SK07_130 chr07 13,548,107 G/C TCTTTCTGGATTATTTGTTGTCCACC (SEQ ID NO: 553) CTTTCTGGATTATTTGTTGTCCACG (SEQ ID NO: 554) GGAGAAATGGCAGCATTAGTTCATACAT (SEQ ID NO: 555) SK07_145 chr07 14,527,854 C/A GACATTTTGGCAACCCTTGCAGC (SEQ ID NO: 556) ATGACATTTTGGCAACCCTTGCAGA (SEQ ID NO: 557) AAATACCTCATGGAGTGGTTCAGACTTAT (SEQ ID NO: 558) SK07_151 chr07 15,119,434 T/G CTCTTTTCACAATTTAGGGGTTCACT (SEQ ID NO: 559) CTCTTTTCACAATTTAGGGGTTCACG (SEQ ID NO: 560) TTCTGCTTACCGAAGTCTTTCTTACTGTAAA (SEQ ID NO: 561) SK07_164 chr07 16,392,426 A/T CTCCTCAATCTACAAACTGATATGCA (SEQ ID NO: 562) CTCCTCAATCTACAAACTGATATGCT (SEQ ID NO: 563) ATATATCTCATGAGCCTAAAAAACTGATAT (SEQ ID NO: 564) SK07_166 chr07 16,611,718 A/T GCGCTTCACCATTTCTCCCTT (SEQ ID NO: 565) CTGCGCTTCACCATTTCTCCCTA (SEQ ID NO: 566) CAGAGCATACCAGTACTGTAATTGACAAA (SEQ ID NO: 567) SK08_001 chr08 89,509 T/A AGATTATGGATACTCATAGTAGCTTACT (SEQ ID NO: 568) AGATTATGGATACTCATAGTAGCTTACA (SEQ ID NO: 569) ACCCTTCCCTCTGCGGCACAA (SEQ ID NO: 570) SK08_008 chr08 777,971 A/C CATGGCCGCCCTCTTTCTTCATT (SEQ ID NO: 571) ATGGCCGCCCTCTTTCTTCATG (SEQ ID NO: 572) CATGTGCGGAGGTAGAAAACAGCAT (SEQ ID NO: 573) SK08_012 chr08 1,153,157 C/T AGAGGTCTCTAACCGGTTATTGTG (SEQ ID NO: 574) GTAGAGGTCTCTAACCGGTTATTGTA (SEQ ID NO: 575) TTTCCATGAAATCAATGCTTTTCCTAGGAA (SEQ ID NO: 576) SK08_020 chr08 2,017,285 T/C GAATTAGTGAAGCAGAAAGATTTGGCA (SEQ ID NO: 577) AATTAGTGAAGCAGAAAGATTTGGCG (SEQ ID NO: 578) ACCCCTCCTGCAATTCCAGCAAAAT (SEQ ID NO: 579) SK08_032 chr08 3,200,726 G/A GGAAGAGCAGGCACCG (SEQ ID NO: 580) AGCTGGAAGGAGCAGGCACCA (SEQ ID NO: 581) GCTCCCCCTATTCAATTTGGGGAAA (SEQ ID NO: 582) SK08_039 chr08 3,894,494 A/G CCTCCGACCGGATAAACTCCA (SEQ ID NO: 583) CCTCCGACCGGATAAACTCCG (SEQ ID NO: 584) CGAGTTTGAAAAGGATCTTGTTTGCAGTA (SEQ ID NO: 585) SK08_046 chr08 4,631,694 C/T AAGGTTTCATTATTAAATATTGCCATAAAAC (SEQ ID NO: 586) CTAAGGTTTCATTATTAAATATTGCCATAAAAT (SEQ ID NO: 587) TAGGCAGCAAGAAAATGAGACACTGAAAA (SEQ ID NO: 588) SK08_052 chr08 5,181,315 C/CTAAG ATACAAGTAATATATGCACTACACACTTAG (SEQ ID NO: 589) ATACAAGTAATATATGCACTACACACTTAC (SEQ ID NO: 590) CAATATCACCACTCAGACAAGAATCGTT (SEQ ID NO: 591) SK08_066 chr08 6,619,677 C/T GATTTGGGCCTTCAATCCTGC (SEQ ID NO: 592) TGGGGCCTTCAATCCTGT (SEQ ID NO: 593) GAAGCACGCCGATGGAAGAGGTT (SEQ ID NO: 594) SK08_072 chr08 7,249,079 T/A AAAAAAGCAAAAAAAGGAGAAACAAATCTTGAA (SEQ ID NO: 595) AAAAAAGCAAAAAAAGGAGAAACAAATCTTGAT (SEQ ID NO: 596) AACTTCCACTCAGAATCATATGACTTGTTT (SEQ ID NO: 597) SK08_125 chr08 12,547,972 A/G ACTGAAAATCGTGCCATTGTGCT (SEQ ID NO: 598) ACTGAAAATCGTGCCATTGTGCC (SEQ ID NO: 599) TACTTGATGGTCCTTGAACGATGGAATTT (SEQ ID NO: 600) SK08_120 chr08 12,682,464 A/C AATTACTTTAAATATTGTCGCAATCAATAATCA (SEQ ID NO: 601) AATTACTTTAAATATTGTCGCAATCAATAATCC (SEQ ID NO: 602) CATAGTATGACGATTTTGGATTCCTTACAT (SEQ ID NO: 603) SK08_137 chr08 13,711,318 A/G ATATAGATTGACAGAGCGTGCCCA (SEQ ID NO: 604) ATAGATTGACAGAGCGTGCCCG (SEQ ID NO: 605) GTTAGTGGGCTTGGTCATTTCATTTACAT (SEQ ID NO: 606) SK08_142 chr08 14,170,972 A/G CAAACAGAGAAACAGAATTATTAGAACTTGT (SEQ ID NO: 607) AAACAGAGAAACAGAATTATTAGAACTTGC (SEQ ID NO: 608) CAGTGGAAATCTTAGTGGAAACCTTAGTA (SEQ ID NO: 609) SK08_148 chr08 14,785,722 C/T GGGGATGTTTTGTATCCGCGAC (SEQ ID NO: 610) CGGGGATGTTTTGTATCCGCGAT (SEQ ID NO: 611) CTGATCCGGAGCCGCTTAATGATTA (SEQ ID NO: 612) SK08_156 chr08 15,627,709 C/A TATAGTGTGAGATCCTTAGTGTATCC (SEQ ID NO: 613) CTTATAGTGTGAGATCCTTAGTGTATCA (SEQ ID NO: 614) CGACCTCAACCATCCAGCTCGAA (SEQ ID NO: 615) SK08_164 chr08 16,356,048 A/G CATTTATTATGCCACCTTTAGGAACAGAA (SEQ ID NO: 616) TATTATGCCACCTTTAGGAACAGAG (SEQ ID NO: 617) GAAGCAGCAATTTTGCAGACCTGCTA (SEQ ID NO: 618) SK08_172 chr08 17,199,524 A/G GTCGAGGAACGACAACAACACGA (SEQ ID NO: 619) CGAGGAACGACAACAACACGG (SEQ ID NO: 620) CGTCAGAGTTATTAGTTACTCTTCCCAAT (SEQ ID NO: 621) SK08_179 chr08 17,925,495 C/G AAATCATTACCTTGTTTCTCGTGCTTG (SEQ ID NO: 622) AAATCATTACCTTGTTTCTCGTGCTTC (SEQ ID NO: 623) GGTTTTACCTCCCATGCAGTCCTT (SEQ ID NO: 624) SK08_187 chr08 18,704,860 A/G AACTACGATGAATTTCAATATGAGAACACT (SEQ ID NO: 625) CTACGATGAATTTCAATATGAGAACACC (SEQ ID NO: 626) CGATTTCGTGATTCTTTTACAGTAAACTAT (SEQ ID NO: 627) SK08_197 chr08 19,693,922 T/A GGATTTAAATGGACATTTAGGTCAATTCAT (SEQ ID NO: 628) TAAATGGACATTTAGGTCAATTCAA (SEQ ID NO: 629) CGATCGTTCAACAATAAATCTATCCCGTT (SEQ ID NO: 630) SK08_203 chr08 20,300,060 G/T GTGAAAATCCATTATACAGATCATCAAAC (SEQ ID NO: 631) CTGTGAAAATCCATTATACAGATCATCAAAA (SEQ ID NO: 632) GCTGTTAAAGATGTATCGAATTCGCAGAA (SEQ ID NO: 633) SK08_209 chr08 20,879,908 A/G CATCATCTTCATCATCTTCTACGTCATAT (SEQ ID NO: 634) CATCTTCATCATCTTCTACGTCATAC (SEQ ID NO: 635) GCGGTCTTTCTAAAAGCAAGTCGATAATTA (SEQ ID NO: 636) SK08_216 chr08 21,593,211 A/T ATTTAGATTTGGGTTCAGGAGCTAAGT (SEQ ID NO: 637) TAGATTTGGGTTCAGGAGCTAAGA (SEQ ID NO: 638) TTTCAACTTTCTATCTTGTTGCGCTGGAT (SEQ ID NO: 639) SK08_219 chr08 21,911,022 A/T AAATGAACACTTATGATGATTAAACTTTTCTTA (SEQ ID NO: 640) AAATGAACACTTATGATGATTAAACTTTTCTTT (SEQ ID NO: 641) TTTGGTCCAGTTTCTCGCACCCTTT (SEQ ID NO: 642) SK08_227 chr08 22,725,920 A/G GATAACCATGTGATCTTTTAACGTCAGT (SEQ ID NO: 643) ATAACCATGTGATCTTTTAACGTCAGC (SEQ ID NO: 644) GATCCATGGACCTATTCATAATACAAGAAT (SEQ ID NO: 645) SK08_233 chr08 23,313,548 A/G ATAGGTCAAAAGAGCGGCGGT (SEQ ID NO: 646) ATAGGTCAAAAGAGCGGCGGC (SEQ ID NO: 647) ATGCCGCTCTCCGCCTCCTTTA (SEQ ID NO: 648) SK08_241 chr08 24,131,699 A/T GCCTGACAGTTCCATTCCAGAATT (SEQ ID NO: 649) GCCTGACAGTTCCATTCCAGAATA (SEQ ID NO: 650) CCATTGGTGTTACAAGATTCTCTTTTCATT (SEQ ID NO: 651) SK08_254 chr08 25,362,212 C/G TCTGCCATGAAAGCAGCGCTG (SEQ ID NO: 652) CTGCCATGAAAGCAGCGCTC (SEQ ID NO: 653) GGAGAACCAAAGGGTGTCATCCTAT (SEQ ID NO: 654) SK08_260 chr08 26,036,321 C/A TTTCATACAGATCGTAGCTAGTTG (SEQ ID NO: 655) CCTTTTCATACAGATCGTAGCTAGTTT (SEQ ID NO: 656) CGGCTGCAGAACATGAGAAACTCAT (SEQ ID NO: 657) SK09_004 chr09 406,629 G/A ATACTACTCCTTCCAGTTTTGGGTC (SEQ ID NO: 658) GATACTACTCCTTCCAGTTTTGGGTT (SEQ ID NO: 659) CTCATCGTTACTTGCAGAGTTTTGATCTT (SEQ ID NO: 660) SK09_028 chr09 2,798,885 C/T ACATTCAGACGACCATAAGTATTAGAC (SEQ ID NO: 661) CACATTCAGACGACCATAAGTATTAGAT (SEQ ID NO: 662) CCTGGATTCGTTCTCTTTCCGTAATTATT (SEQ ID NO: 663) SK09_035 chr09 3,454,376 C/A TTTACAATTTCTTAGATTCCATGGTATTG (SEQ ID NO: 664) AACTTTTACAATTTCTTAGATTCCATGGTATTT (SEQ ID NO: 665) GAGAACTTATTGACACTAGAATCTAGCATA (SEQ ID NO: 666) SK09_042 chr09 4,244,199 G/A GTAGAAGTCAAAGATGGATAATATGGTTC (SEQ ID NO: 667) GTAGAAGTCAAAGATGGATAATATGGTTT (SEQ ID NO: 668) TGAGTCCATCAAAATCACACATCACCTAT (SEQ ID NO: 669) SK09_052 chr09 5,167,485 T/A ATTTCTCTGTGACTCCGTTTCAACAT (SEQ ID NO: 670) TCTCTGTGACTCCGTTTCAACAA (SEQ ID NO: 671) CTGCGGAACATTTGGTGGTTCAAAATATT (SEQ ID NO: 672) SK09_056 chr09 5,579,088 A/G AAAAGCAACTTCCCGAATTCGAGAAAA (SEQ ID NO: 673) AAGCAACTTCCCGAATTCGAGAAAG (SEQ ID NO: 674) GTGTTTGCAGTAGATAGTTCAAATTCAGTT (SEQ ID NO: 675) SK09_062 chr09 6,204,619 T/C CTTGTTGCTTAGCCAATGGCAACT (SEQ ID NO: 676) GTTGCTTAGCCAATGGCAACC (SEQ ID NO: 677) GTCCGAGTCTGGAAGACAGAACTAT (SEQ ID NO: 678) SK09_068 chr09 6,761,439 T/C GGCATAAGTAATATAGCACAGACTGTAA (SEQ ID NO: 679) GCATAAGTAATATAGCACAGACTGTAG (SEQ ID NO: 680) GATTCTTGTAGCAGACTGTCCAATACTTT (SEQ ID NO: 681) SK09_075 chr09 7,548,750 G/A ATTTAAAAATACTCACAGTATCCATCTGG (SEQ ID NO: 682) TTATTTAAAAATACTCACAGTATCCATCTGA (SEQ ID NO: 683) CGGAGTAGAGACAAAAATGCATGGCTT (SEQ ID NO: 684) SK09_093 chr09 9,331,889 T/C CAAAAATTTTATATATAACTGCACCAAGTCATA (SEQ ID NO: 685) AAAAATTTTATATATAACTGCACCAAGTCATG (SEQ ID NO: 686) CATTATGATTAAACCGGAAAGGTAAAGGAA (SEQ ID NO: 687) SK09_123 chr09 12,321,445 A/G CATGAACTTCATGACGCAACAAACGA (SEQ ID NO: 688) ATGAACTTCATGACGCAACAAACGG (SEQ ID NO: 689) CGGCGGTTGTAGAGGTTCCCCAT (SEQ ID NO: 690) SK09_132 chr09 13,236,966 A/G CGTTAGGTTTGGACGAAAAAATACTGAA (SEQ ID NO: 691) GTTAGGTTTGGACGAAAAATACTGAG (SEQ ID NO: 692) ATTCCTCAAGAATGTTAAATAAGAGGTAAA (SEQ ID NO: 693) SK09_140 chr09 14,040,195 G/T AAGGATAAAGGAAAGATGGCGGC (SEQ ID NO: 694) CAAGGATAAAGGAAAGATGGCGGA (SEQ ID NO: 695) GTTCTGTACAACTCATCATCTCCTTGTAT (SEQ ID NO: 696) SK09_154 chr09 15,371,976 C/G CCGTTAACCAAAGACATTACTGCTG (SEQ ID NO: 697) CCGTTAACCAAAGACATTACTGCTC (SEQ ID NO: 698) AGAAGAAGTAATGGGAAGAGTCCAGTTT (SEQ ID NO: 699) SK09_162 chr09 16,182,402 T/G CAAGGGTTGTACTTCTTCTCAGAT (SEQ ID NO: 700) CAAGGGTTGTACTTCTTCTCAGAG (SEQ ID NO: 701) CTGTGATGGCTCAAATCCATGATCAAAT (SEQ ID NO: 702) SK09_160 chr09 16,350,592 T/A CACTGTATGGTGAATTGAATCTGCTT (SEQ ID NO: 703) CACTGTATGGTGAATTGAATCTGCTA (SEQ ID NO: 704) TCTATCCTACTCCACAATTGATCCTCTTT (SEQ ID NO: 705) SK09_169 chr09 16,851,250 G/A TATAAGCCTAACTGTCTTTGCTTTC (SEQ ID NO: 706) GCTTATAAGCCTAACTGTCTTTGCTTTT (SEQ ID NO: 707) GCCTCGTTGAATTCAGAAGCGCAAA (SEQ ID NO: 708) SK09_173 chr09 17,286,182 C/A GGGCGGAGAAAAGACATGAGC (SEQ ID NO: 709) GGGGCGGAGAAAAGACATGAGA (SEQ ID NO: 710) CTCGGCTCCTGGCTCTCCAAAT (SEQ ID NO: 711) SK09_177 chr09 17,712,941 C/T TTCTTCAGAAGCTCTGTTTTTATTACTTC (SEQ ID NO: 712) TTTTCTTCAGAAGCTCTGTTTTTATTACTTT (SEQ ID NO: 713) AGAATAGCAACATTAGGTCGAGCTTCAAA (SEQ ID NO: 714) SK09_181 chr09 18,050,446 G/A ACCCCAGTATTTAAGGAGATGG (SEQ ID NO: 715) GCTACCCCAGTATTTAAGGAGATGA (SEQ ID NO: 716) AGTCTTCTTGCATAGGCACAAACTCATTT (SEQ ID NO: 717) SK09_186 chr09 18,593,673 C/G CAAAATCCCTTCAATTATCATTTTACGAG (SEQ ID NO: 718) CAAAATCCCTTCAATTATCATTTTACGAC (SEQ ID NO: 719) GTCTCGATCTATCCTGATATTGGACAAA (SEQ ID NO: 720) SK09_189 chr09 18,905,618 C/T AACCAGATGCGGACCGAATCAG (SEQ ID NO: 721) AAACCAGATGCGGACCGAATCAA (SEQ ID NO: 722) AAATCAGCATATTCCTCCCATAAAGACAAA (SEQ ID NO: 723) SK09_197 chr09 19,698,199 A/T CTCATGCCTTTTTCTGTCAACCCA (SEQ ID NO: 724) CTCATGCCTTTTTCTGTCAACCCT (SEQ ID NO: 725) CCTGGTTTAACATCAAGAATCAGAAGGAA (SEQ ID NO: 726) SK09_208 chr09 20,770,061 T/C GTTCAACCGTATCTCTTCTTCTCAAT (SEQ ID NO: 727) GTTCAACCGTATCTCTTCTTCTCAAC (SEQ ID NO: 728) GACGAATCGAAAAATAAGCATACACGCAT (SEQ ID NO: 729) SK09_213 chr09 21,326,539 A/C GTAGTTGCCTTTTTGACGCCGTAAA (SEQ ID NO: 730) AGTTGCCTTTTTGACGCCGTAAC (SEQ ID NO: 731) CTCCTGGCTTTTTATGCTTGTATGGATAA (SEQ ID NO: 732) SK09_224 chr09 22,364,883 G/T ATATCGGAAAGTGGAAACAAGCAAAAC (SEQ ID NO: 733) GATATCGGAAAGTGGAAACAAGCAAAAA (SEQ ID NO: 734) GCCCACATCTGGGAACTCATTATAAATTT (SEQ ID NO: 735) SK09_228 chr09 22,841,601 G/A CAAAATCAAGCCTGTCATCATTTCTAAG (SEQ ID NO: 736) ATCAAAATCAAGCCTGTCATCATTTCTAAA (SEQ ID NO: 737) GTCTGTAACTTGATACGATAAAGTGGCTT (SEQ ID NO: 738) SK10_005 chr10 510,171 A/G CGTCGTCGTCGATGATAAATGGT (SEQ ID NO: 739) CGTCGTCGTCGATGATAAATGGC (SEQ ID NO: 740) GGATGAGCATGAACGCGGCATTAAT (SEQ ID NO: 741) SK10_011 chr10 1,079,534 A/T GTAGGATCAAATCAGCATCGTTTGAA (SEQ ID NO: 742) GTAGGATCAAATCAGCATCGTTTGAT (SEQ ID NO: 743) GAGAGGTAGGGGCAAAATGGGAAAT (SEQ ID NO: 744) SK10_014 chr10 1,414,235 G/C AAATTGTATATGAGAATGCTTAATACAATATGC (SEQ ID NO: 745) AAATTGTATATGAGAATGCTTAATACAATATGG (SEQ ID NO: 746) CTTTGGTTGTTTGCGTGACTATGAACATT (SEQ ID NO: 747) SK10_019 chr10 1,925,441 T/G GAAATCTCCTCCGATGATGAGGAA (SEQ ID NO: 748) AAATCTCCTCCGATGATGAGGAC (SEQ ID NO: 749) GTAGGAACGGCTAGTCCTCGGAA (SEQ ID NO: 750) SK10_028 chr10 2,779,024 G/A AAATCTGAGCTCGAGCTCGACG (SEQ ID NO: 751) AAAAATCTGAGCTCGAGCTCGACA (SEQ ID NO: 752) CTCGAGTTCAAGCTCGAGCCTTTAT (SEQ ID NO: 753) SK10_032 chr10 3,207,586 G/A ATAATGCACCTGATCCGCAGCC (SEQ ID NO: 754) ATAATGCACCTGATCCGCAGCT (SEQ ID NO: 755) TAGAGGATGTGAATGATGAGGTGGTTTT (SEQ ID NO: 756) SK10_038 chr10 3,790,092 G/T CACTAACAAGAACAAGCCGGGG (SEQ ID NO: 757) AACACTAACAAGAACAAGCCGGGT (SEQ ID NO: 758) CGGGTGATTTTGGCTCTCCCAAAAA (SEQ ID NO: 759) SK10_046 chr10 4,622,752 T/C AACACAACAACTATCAGAAAATGTTCCAT (SEQ ID NO: 760) CACAACAACTATCAGAAAATGTTCCAC (SEQ ID NO: 761) CTCGTTTCTAATTGTCGTAGCTATTGGTT (SEQ ID NO: 762) SK10_053 chr10 5,326,654 C/CA GAAAGTGCTCTCATCTTTACATACAAAT (SEQ ID NO: 763) GAAAGTGCTCTCATCTTTACATACAAAA (SEQ ID NO: 764) CCCTCTCTGCCTTCATTTCTTCAGAT (SEQ ID NO: 765) SK10_061 chr10 6,143,426 C/G TTCCCCAAAACGAGCTTCC (SEQ ID NO: 766) GCTTTCCCCAAAACGAGCTTCG (SEQ ID NO: 767) CCTCACACGAATTATGGCTCGAGTT (SEQ ID NO: 768) SK10_076 chr10 7,619,549 G/A TGCTCTTGTAATATGGGTCATCAG (SEQ ID NO: 769) CTTGCTCTTGTAATATGGGTCATCAA (SEQ ID NO: 770) CCCTGTGTGCCATATGAACTTCCAA (SEQ ID NO: 771) SK10_086 chr10 8,559,283 A/C TCAGGATGTGGGGTTTCGGTTTA (SEQ ID NO: 772) CAGGATGTGGGGTTTCGGTTTC (SEQ ID NO: 773) CAATAATTTGGTAAGTGGGTCTGTTTTGAT (SEQ ID NO: 774) SK10_092 chr10 9,241,537 C/T AATCAAGCCTAAATGGCCACTCG (SEQ ID NO: 775) CAATCAAGCCTAAATGGCCACTCA (SEQ ID NO: 776) GGTCCATGGAAAAAGAAGTTTGCATTCAT (SEQ ID NO: 777) SK10_097 chr10 9,703,770 C/T ATGGAAATGGAATTCTACTGCAC (SEQ ID NO: 778) GCTATGGAAATGGAATTCTACTGCAT (SEQ ID NO: 779) CTGCAATAGCAGAATCATGGGAATTCTAA (SEQ ID NO: 780) SK10_113 chr10 11,323,106 C/T ACCTAAAATCCACTTCTGAAGTATTC (SEQ ID NO: 781) AACTACCTAAAATCCACTTCTGAAGTATTT (SEQ ID NO: 782) CGTCTCGAATTCATTTTATTTGGGACAGAT (SEQ ID NO: 783) SK10_123 chr10 12,277,919 C/T TAGCAATAATTCAGATACGTTCCTCG (SEQ ID NO: 784) GTTAGCAATAATTCAGATACGTTCCTCA (SEQ ID NO: 785) TCAATTCTGCAGCCTGAGTAATGGAATT (SEQ ID NO: 786) SK10_128 chr10 12,805,341 T/C TTAAGTAATTGCTGGGTTTGTTCGGA (SEQ ID NO: 787) AAGTAATTGCTGGGTTTGTTCGGG (SEQ ID NO: 788) GAAGCAAACACCAGAAACATGCTAAACAA (SEQ ID NO: 789) SK10_131 chr10 13,142,419 C/T ACAAACTAGTTTAATTAATAATATACCCATTGC (SEQ ID NO: 790) ACAAACTAGTTTAATTAATAATATACCCATTGT (SEQ ID NO: 791) CTCTTGATGATATGAGGCAATGGATCTAA (SEQ ID NO: 792) SK10_135 chr10 13,507,462 G/C TTTTAAGGGGAACTTTTCAATTTTTTTTTTCC (SEQ ID NO: 793) TTTAAGGGGAACTTTTCAATTTTTTTTTTCG (SEQ ID NO: 794) TACTCTCAGGGACTCTTATAACTAACCTT (SEQ ID NO: 795) SK10_146 chr10 14,586,802 C/A ATTCTTGCTTTGCACCGCCG (SEQ ID NO: 796) GCTATTCTTGCTTTGCACCGCCT (SEQ ID NO: 797) ACACCAGGTGGCTGCCGCTAT (SEQ ID NO: 798) SK10_153 chr10 15,294,110 T/A GGCCAACGTGCAATCCACT (SEQ ID NO: 799) CTGGCCAACGTGCAATCCACA (SEQ ID NO: 800) CCTTCAGCGGGACCTCCAGTTT (SEQ ID NO: 801) SK10_161 chr10 16,070,467 G/C ACCAATACAAACTTGCATGTCCCG (SEQ ID NO: 802) ACCAATACAAACTTGCATGTCCCC (SEQ ID NO: 803) GGTGGAGCTTTTTGGCCAAGTTGAA (SEQ ID NO: 804) SK10_167 chr10 16,736,530 G/A GGCCGGAGATTGCAGCAGC (SEQ ID NO: 805) ATGGCCGGAGATTGCAGCAGT (SEQ ID NO: 806) CGTTTCCATCTCCATCCACAGTCTA (SEQ ID NO: 807) SK10_174 chr10 17,415,055 C/T CCAAGTCCAAACGTCACCAGC (SEQ ID NO: 808) TCCAAGTCCAAACGTCACCAGT (SEQ ID NO: 809) CATATGCTCCTGCAGATTGGCCAAA (SEQ ID NO: 810) SK10_182 chr10 18,178,475 T/A ACAAACACCATCCCAATATCTAGCAAT (SEQ ID NO: 811) ACAAACACCATCCCAATATCTAGCAAA (SEQ ID NO: 812) GTGCTGATTCTTCTCAAATTCCTCGTAAA (SEQ ID NO: 813) SK11_001 chr11 68,940 A/G GATGGACTGATATCGCAAATCAACCA (SEQ ID NO: 814) ATGGACTGATATCGCAAATCAACCG (SEQ ID NO: 815) GCAATTTCAGGGACTGGTACGACTT (SEQ ID NO: 816) SK11_007 chr11 741,853 A/G TTGAACAAGACTGACAAGGTTAT (SEQ ID NO: 817) GCTTTGAACAAGACTGACAAGGTTAC (SEQ ID NO: 818) GGCTTTTCATCTGCATTCTGGGATGAT (SEQ ID NO: 819) SK11_015 chr11 1,548,179 T/C TTCAGTACAAGACGCTGCTGAAGT (SEQ ID NO: 820) CAGTACAAGACGCTGCTGAAGC (SEQ ID NO: 821) CAGAGTAGCCGCTCGGACATGTT (SEQ ID NO: 822) SK11_022 chr11 2,193,536 G/A TCCCCTTCATTTTCCCCCTCC (SEQ ID NO: 823) CTTCCCCTTCATTTTCCCCCTCT (SEQ ID NO: 824) CCACTGTTGTTGCTCATCGTCTGAT (SEQ ID NO: 825) SK11_027 chr11 2,738,504 A/G ATAATGTTGGCTTCCATCGGCAACT (SEQ ID NO: 826) ATGTTGGCTTCCATCGGCAACC (SEQ ID NO: 827) TTAATTACCTGATGTTGGGATCCACGTATT (SEQ ID NO: 828) SK11_035 chr11 3,455,009 A/G TAGACTGTCTTCACAGTCGCTAGT (SEQ ID NO: 829) GACTGTCTTCACAGTCGCTAGC (SEQ ID NO: 830) CAGCCACTTTCCCCTCTCCTGAA (SEQ ID NO: 831) SK11_042 chr11 4,152,876 C/T TGATTTACTTCGAACCCTCCTTCG (SEQ ID NO: 832) GCTTGATTTACTTCGAACCTCCTTCA (SEQ ID NO: 833) ACGTTGAAGTTTCGCGTGAACAGATTAAA (SEQ ID NO: 834) SK11_050 chr11 5,032,797 T/C CTGGAAAACCCCTAATTTGGCCTA (SEQ ID NO: 835) GGAAAACCCCTAATTTGGCCTG (SEQ ID NO: 836) GGTATGAGAGGTGTATGGGTACAGTT (SEQ ID NO: 837) SK11_059 chr11 5,895,979 C/G CTCACTAAAAAGCAAACATAAATCAAATCG (SEQ ID NO: 838) CTCACTAAAAGCAAACATAAATCAAATCC (SEQ ID NO: 839) CACTCGGCCACAAAATCTCCTTAGAT (SEQ ID NO: 840) SK11_066 chr11 6,572,091 G/A GTGAGAAAGAGTGAGGTTTCTTGC (SEQ ID NO: 841) AGGTGAGAAAGAGTGAGGTTCTTGT (SEQ ID NO: 842) ACATCCCACCAATTAAGCAATGGATTCTT (SEQ ID NO: 843) SK11_073 chr11 7,258,109 C/G AGCTTATAAGCATTTTAGCATCTGGAC (SEQ ID NO: 844) AGCTTATAAGCATTTTAGCATCTGGAG (SEQ ID NO: 845) GTTCACCAATATTCTGGGAGTAATTGCAA (SEQ ID NO: 846) SK11_084 chr11 8,399,193 C/T GAAACTCCCACCAAAGACGGTG (SEQ ID NO: 847) GAAACTCCCACCAAAGACGGTA (SEQ ID NO: 848) GCCGGCGTGTCACTAGAATCCAT (SEQ ID NO: 849) SK11_092 chr11 9,225,362 G/A CTTGATCCGACAAGTCTGCC (SEQ ID NO: 850) CTCTTGATCCGACAAGTCTGCT (SEQ ID NO: 851) GCATTCTTTGTTGGTTTGGACAAAACCAT (SEQ ID NO: 852) SK11_102 chr11 10,175,290 T/G GTGGTACCTGTACGGTCACCTA (SEQ ID NO: 853) GGTACCTGTACGGTCACCTC (SEQ ID NO: 854) GGCAAAAATGAGCTGGCCCATCAAA (SEQ ID NO: 855) SK11_109 chr11 10,910,922 C/T GATCGATCTTGTTACCACTTTATAACG (SEQ ID NO: 856) GGATCGATCTTGTTACCACTTTATAACA (SEQ ID NO: 857) ACCGTCCATCTAAATCACAACGTATTGTA (SEQ ID NO: 858) SK11_117 chr11 11,677,450 C/T AGCCAAACGAATCTTAAATAAATGAATTGC (SEQ ID NO: 859) GAGCCAAACGAATCTTAAATAAATGAATTGT (SEQ ID NO: 860) CCATTACTTTGTAGTTCCGTGTGGAATA (SEQ ID NO: 861) SK11_125 chr11 12,500,660 T/C TTAAATGAAATGTTCATTCCATACGCCAT (SEQ ID NO: 862) AAATGAAATGTTCATTCCATACGCCAC (SEQ ID NO: 863) AGTGGTGTTCAAAACCTTGTAAGAGATTTT (SEQ ID NO: 864) SK11_130 chr11 13,038,002 G/A CCATCTTTGAGCTACCAACTTAAGTG (SEQ ID NO: 865) CCATCTTTGAGCTACCAACTTAAGTA (SEQ ID NO: 866) CAGCAATGATGATGATGATGAGATGAGAA (SEQ ID NO: 867) SK11_137 chr11 13,668,839 T/C GACCTTATTAGTCAAAGAACATCCTCA (SEQ ID NO: 868) ACCTTATTAGTCAAAGAACATCCTCG (SEQ ID NO: 869) CTACAACTTCTTTTTCAGGTGCTGACATA (SEQ ID NO: 870) SK12_007 chr12 732,049 /A CAATTTGCAAGTTCCTATCTTCATTTTTTTG (SEQ ID NO: 871) CCAATTTGCAAGTTCCTATCTTCATTTTTTTT (SEQ ID NO: 872) CCCTCTATAATATTGTAAATGGGTAAATTA (SEQ ID NO: 873) SK12_019 chr12 1,865,901 A/C GCAACGTAGGCTTGTTGGAGGA (SEQ ID NO: 874) CAACGTAGGCTTGTTGGAGGC (SEQ ID NO: 875) CATGTGAGCAAGAATGCTACAAACCAAA (SEQ ID NO: 876) SK12_022 chr12 2,220,408 C/T GGTTGTTTACTTATTGAGTCATGCAAC (SEQ ID NO: 877) CGGTTGTTTACTTATTGAGTCATGCAAT (SEQ ID NO: 878) GTATATAGAATTCAATGTCTACACCTGAAA (SEQ ID NO: 879) SK12_033 chr12 3,344,561 A/G GAGTATAAATTGTATACAACACCCCCT (SEQ ID NO: 880) AGTATAAATTGTATACAACACCCCCC (SEQ ID NO: 881) GGGATCAAGTCATAACGTAGTTCAAACAA (SEQ ID NO: 882) SK12_042 chr12 4,168,027 G/C CGTCCATAGATCCAGTTCTTCTG (SEQ ID NO: 883) CGTCCATAGATCCAGTTCTTCTC (SEQ ID NO: 884) ACATCCAAATGAAAATCAAGGTGGAGATAT (SEQ ID NO: 885) SK12_052 chr12 5,236,352 C/G ACGGTACTACACAAACCTTTGGTC (SEQ ID NO: 886) ACGGTACTACACAAACCTTTGGTG (SEQ ID NO: 887) GCAACAACTACTTTTGGGTTTGTCAACAA (SEQ ID NO: 888) SK12_066 chr12 6,643,164 C/A TTATCCATCATACACTATGTATAAAGTGC (SEQ ID NO: 889) CTTTTATCCATCATACACTATGTATAAAGTGA (SEQ ID NO: 890) AGGGGACATTTTCTTGTGGTTTATAGTTTA (SEQ ID NO: 891) SK12_073 chr12 7,278,578 C/T AAGAAAAAAAGAAAGAAGAAAGCAAGCAAC (SEQ ID NO: 892) AAAAAGAAAAAAAGAAAGAAGAAAGCAAGCAAT (SEQ ID NO: 893) TGTTATGCTAATAATGCCCAAAGTTGATTT (SEQ ID NO: 894) SK12_080 chr12 8,028,278 T/C GTAAACATTCTCCTGATAAACTAATGTGTTA (SEQ ID NO: 895) AAACATTCTCCTGATAAACTAATGTGTTG (SEQ ID NO: 896) CTACAACAGGGAAGATTCTGAATTGGTAT (SEQ ID NO: 897) SK12_089 chr12 8,899,309 C/T GTATCGAATATAATTTCTGAACATGAATACC (SEQ ID NO: 898) GGTATCGAATATAATTTCTGAACATGAATACT (SEQ ID NO: 899) GATAGGCAGGTCCATGGATAGAGTT (SEQ ID NO: 900) SK12_096 chr12 9,573,753 G/A GTGATGGCCCAGTCCAAGCAAG (SEQ ID NO: 901) GTGATGGCCCAGTCCAGCAAA (SEQ ID NO: 902) GGGCTAGTTTTATTCTCTTAATGCACCTT (SEQ ID NO: 903) SK12_110 chr12 11,078,054 T/C CAGTTTCAATCATGTCACTTTTCCCA (SEQ ID NO: 904) CAGTTTCAATCATGTCACTTTTCCCG (SEQ ID NO: 905) CATATTCCAGCACCTATCCTGTCAATAAT (SEQ ID NO: 906) SK12_118 chr12 11,823,300 T/C GACATCCTAAACCCTTTTCTTTATAAACTT (SEQ ID NO: 907) ACATCCTAAACCCTTTTCTTTATAAACTC (SEQ ID NO: 908) GTGTTTGGTTTTCCTTTGTTTAACCAGTTT (SEQ ID NO: 909) SK12_123 chr12 12,339,675 T/C GGCCGCCTTCCTCAATGATAGT (SEQ ID NO: 910) GCCGCCTTCCTCAATGATAGC (SEQ ID NO: 911) TGAGTAGCTTGGCTGGGATTGAGAT (SEQ ID NO: 912) SK12_128 chr12 12,777,808 C/A CTGATGCGAGTAGATTCTGCC (SEQ ID NO: 913) CTCTGATGCGAGTAGATTCTGCA (SEQ ID NO: 914) CTGTCCTTATCCGTCATGTACTAATGATT (SEQ ID NO: 915) SK12_134 chr12 13,397,882 C/T TTATACCTATAATTGTTAATGATTGATATCC (SEQ ID NO: 916) CTTTATACCTATAATTGTTAATGATTGATATCT (SEQ ID NO: 917) CGAGTCTCAACCAGATCAATGCCAA (SEQ ID NO: 918) SK12_138 chr12 13,828,946 C/T CAGGCATCGTTCTGTAAGGCG (SEQ ID NO: 919) CTCAGGCATCGTTCTGTAAGGCA (SEQ ID NO: 920) ACCGTTGGCATTTGCTGCCATATTTATTT (SEQ ID NO: 921) SK12_147 chr12 14,735,068 T/G CGGAGTTGCTACCCAATGCAGT (SEQ ID NO: 922) GGAGTTGCTACCCAATGCAGG (SEQ ID NO: 923) GCGCTGTGTTATCCTCAGCTAACAT (SEQ ID NO: 924) SK12_151 chr12 15,084,500 C/T AACAAAAAGGGAACCAAAAAGCATGAC (SEQ ID NO: 925) CAACAAAAAGGGAACCAAAAAGCATGAT (SEQ ID NO: 926) CTCTCAGAATCCAATTTCACAGCCTTAAT (SEQ ID NO: 927) SK12_161 chr12 16,063,033 G/A CAAAGCGACGGAGGCTTTGAC (SEQ ID NO: 928) GCAAAGCGACGGAGGCTTTGAT (SEQ ID NO: 929) GAATCGGCCCTCAGGGCTCTAA (SEQ ID NO: 930) SK13_003 chr13 327,003 G/T CAATGAAATTTAAGAAGTTGTGAATGGCC (SEQ ID NO: 931) CAATGAAAATTTAAGAAGTTGTGAATGGCA (SEQ ID NO: 932) CACCCTCAATCTCATCCAGCTTTGTT (SEQ ID NO: 933) SK13_008 chr13 837,612 T/G CATTGCCTTGGTCAAACAAATAATATT (SEQ ID NO: 934) CTCATTGCCTTGGTCAAACAAATAATATG (SEQ ID NO: 935) CAAAGCAATGAACATGAAAAGTGTCCTATA (SEQ ID NO: 936) SK13_015 chr13 1,548,753 C/T CCCAAGTCTCGAGAAGAGGAG (SEQ ID NO: 937) TCCCAAGTCTCGAGAAGAGGAA (SEQ ID NO: 938) CTCCAATCGGCTTTGCTCGTCTTT (SEQ ID NO: 939) SK13_023 chr13 2,334,202 G/C GTGCAAACACTAAGCTCTCCTC (SEQ ID NO: 940) GTGCAAACACTAAGCTCTCCTG (SEQ ID NO: 941) TCTGTCGGAAGCAGGGCTTAGATA (SEQ ID NO: 942) SK13_055 chr13 5,511,400 C/T AGATATATTTGTTAGACGAAAGCAAACATC (SEQ ID NO: 943) ATAGATATATTTGTTAGACGAAAGCAAACATT (SEQ ID NO: 944) GACCACTCTTGTTGTTTTGAAAAACTACAT (SEQ ID NO: 945) SK13_061 chr13 6,052,690 G/A CCAAATTATTCTCATACGTCTTTCCATG (SEQ ID NO: 946) AACCAAATTATTCTCATACGTCTTTCCATA (SEQ ID NO: 947) CGTAACTTGTTATTATTATCTATTCGTGAA (SEQ ID NO: 948) SK13_067 chr13 6,716,292 A/G AAAAATGATCATTGGTTAATTAAGTACGAGAA (SEQ ID NO: 949) AAATGATCATTGGTTAATTAAGTACGAGAG (SEQ ID NO: 950) CACCAACTCTGAACTGTTTTTGTGATGAT (SEQ ID NO: 951) SK13_071 chr13 7,140,176 CTCT/C ATTATTTATAATTACTCCAAAGTTGCTCTTCTT (SEQ ID NO: 952) ATTTATAATTACTCCAAAGTTGCTCTTCTA (SEQ ID NO: 953) CCGCACAGACGATTATGAGCTGAAA (SEQ ID NO: 954) SK13_080 chr13 7,967,744 A/G CACATAAGGACTAAAACTGAGATTAGGA (SEQ ID NO: 955) ACATAAGGACTAAAACTGAGATTAGGG (SEQ ID NO: 956) CACAGAACAATCCCTTTTGCACTCTAAAA (SEQ ID NO: 957) SK13_088 chr13 8,763,227 ATCAGC/A TTATATAAGGAAAAAAATCCAATTCATTATCA (SEQ ID NO: 958) TATATAAGGAAAAAAAATCCAATTCATTATCG (SEQ ID NO: 959) GGCTGCAAATCAAAACCAGGAAATTCATA (SEQ ID NO: 960) SK13_094 chr13 9,353,159 CT/C ACTTACTGTCAAACTGGTTAAGGGAAA (SEQ ID NO: 961) CTTACTGTCAAACTGGTTAAGGGAAG (SEQ ID NO: 962) GACGTAGTGATTCTAGTAATTCCTGCAAT (SEQ ID NO: 963) SK13_103 chr13 10,272,114 A/C GCAGTCAGAAATGGGAGAATCTGAT (SEQ ID NO: 964) CAGTCAGAAATGGGAGAATCTGAG (SEQ ID NO: 965) GGGCTGCGGCGCTGGGAAT (SEQ ID NO: 966) SK13_107 chr13 10,720,845 C/T CCTATCTGGTGAGGAGTCTC (SEQ ID NO: 967) GCTCCTATCTGGTGAGGAGTCTT (SEQ ID NO: 968) TCAGAGGGATGTACTCCGTAGCTT (SEQ ID NO: 969) SK13_113 chr13 11,255,678 G/C TTGACGACTGATTGGCGAGGG (SEQ ID NO: 970) TGACGACTGATTGGCGAGGC (SEQ ID NO: 971) AAAACAGATAACCTGATTTCTCCAGCAAAA (SEQ ID NO: 972) SK13_123 chr13 12,287,799 C/T AAAGTTTGGGTGTTTGAGCCTCC (SEQ ID NO: 973) GAAAGTTTGGGTGTTTGAGCCTCT (SEQ ID NO: 974) GATGTGCACAGGAGACCCAACATAT (SEQ ID NO: 975) SK13_127 chr13 12,731,438 T/C GTCTGTGGTTAGCCTCTGGGAA (SEQ ID NO: 976) CTGTGGTTAGCCTCTGGGAG (SEQ ID NO: 977) CAAAGCTAAGAAGTACAAAACATCACATTT (SEQ ID NO: 978) SK13_131 chr13 13,122,045 G/A AGTTGGTCGCTTCAACTGCTAAAAC (SEQ ID NO: 979) GAGTTGGTCGCTTCAACTGCTAAAAT (SEQ ID NO: 980) CCAGAGAGTGTTTTTTCAGTGTGATCATA (SEQ ID NO: 981) SK13_138 chr13 13,796,982 CTCTA/C ATGGGCGTTACCTCACAAGAAAGAT (SEQ ID NO: 982) GGGCGTTACCTCACAAGAAAGAG (SEQ ID NO: 983) CGGGTGCAAGCTATCAGCTATGATA (SEQ ID NO: 984) SK13_150 chr13 15,045,055 A/C AGTCCATGCACATTTAAATATACAAAATCTA (SEQ ID NO: 985) GTCCATGCACATTTAAATATACAAAATTCTC (SEQ ID NO: 986) CCCCACTTAGTCTCCTTGTTGAGAA (SEQ ID NO: 987) SK13_157 chr13 15,656,346 G/A ATTGGACCAAAATTGCTACTATTCAAAC (SEQ ID NO: 988) ATATATTGGACCAAAATTGCTACTATTCAAAT (SEQ ID NO: 989) TCCAATACTACTAGTAGCTATAAATGCCAA (SEQ ID NO: 990) SK13_164 chr13 16,375,392 C/T ATTTTTTTCTTAAAATGCCAAAGTCACTATTTG (SEQ ID NO: 991) TTTTTCTTAAAATGCCAAAGTCACTATTTA (SEQ ID NO: 992) GCTCGATGAACAAACGAAACTAGAATTAAA (SEQ ID NO: 993)

Example 4: Analyzing 46 Sesame Varieties Affinity Using 331 KASP Markers

The results of analyzing the relatedness using the genotype information of 46 breeds profiling using 331 markers are shown in FIG. 5. The phylogeny analysis was performed by the neighbor joining method using Mega X software.

There are two main groups, which are divided into Group A, which includes 38 varieties, and Group B, which contains only 8 varieties, such as Keumok and Nuri.

Example 5: Selection of key markers for distinguishing 46 varieties of sesame seeds

We selected the minimum key markers to distinguish 46 domestic sesame varieties. A publicly available heuristic algorithm-based PoweCore program (http://genebank.rda.go.kr/powercore.do) was used.

19 key markers were selected from a total of 331 markers, and the locations of these key markers and the types of genetic mutations are shown in Table 3 below. For primer sequence information, you can refer to the marker information indicated in bold in Table 2.

order marker name chromosome location gene SNP/InDel Bases One SK01_160 chr01 15976845 Intron SNPs [C/T] 2 SK02_022 chr02 2176358 Intergenic SNPs [T/G] 3 SK02_036 chr02 3646839 Intergenic SNPs [C/T] 4 SK03_252 chr03 25218994 Non_synonymous SNPs [A/C] 5 SK04_158 chr04 15783359 Intron SNPs [T/C] 6 SK04_200 chr04 19985006 Synonymous SNPs [C/T] 7 SK05_059 chr05 5867130 Intergenic SNPs [C/T] 8 SK06_013 chr06 1319683 Intron SNPs [T/A] 9 SK06_162 chr06 16161699 Synonymous SNPs [T/G] 10 SK07_073 chr07 7277884 Intron SNPs [A/T] 11 SK08_039 chr08 3894494 Non_synonymous SNPs [A/G] 12 SK09_186 chr09 18593673 Intergenic SNPs [C/G] 13 SK10_061 chr10 6143426 Non_synonymous SNPs [C/G] 14 SK10_076 chr10 7619549 Synonymous SNPs [G/A] 15 SK11_001 chr11 68940 Intergenic SNPs [A/G] 16 SK12_042 chr12 4168027 Intron SNPs [G/C] 17 SK12_096 chr12 9573753 Intergenic SNPs [G/A] 18 SK12_147 chr12 14735068 Non_synonymous SNPs [T/G] 19 SK13_094 chr13 9353159 Intron InDel [CT/C]

Figure 6 shows the phylogeny and genotype of 46 domestic sesame varieties using only 19 key markers. It is possible to classify 46 breeds using only key markers, and similarly to the case of using 331 markers in the phylogenetic tree analysis, they were grouped into main group A, which includes a large number of breeds, and group B, which includes a minority breed.

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention may be embodied in other specific forms without changing the technical spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not limiting. The scope of the present invention should be construed as including all changes or modifications derived from the meaning and scope of the following claims and their equivalent concepts rather than the detailed description above.

<110> REPUBLIC OF KOREA (MANAGEMENT: RURAL DEVELOPMENT ADMINISTRATION) <120> Competitive allele specific PCR primer set for distinguishing sesame cultivars, constructing genetic map of red bean and use its <130> KPA211764-KR <160> 993 <170> KoPatentIn 3.0 <210> 1 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK01_007 <400> 1 acatggacgt ctaacaagag cttg 24 <210> 2 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK01_007 <400> 2 acatggacgt ctaacaagag ctta 24 <210> 3 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK01_007 <400> 3 ctgcgatata tattggtacc tactgtgaa 29 <210> 4 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK01_012 <400> 4 attcacaacc ccaaaaaaca aaagtgaat 29 <210> 5 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK01_012 <400> 5 cacaacccca aaaaacaaaa gtgaac 26 <210> 6 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK01_012 <400> 6 ttgcatgtgg atgatgttat gtcagtgat 29 <210> 7 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK01_018 <400> 7 aaactcagct gggattcttc ctgt 24 <210> 8 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK01_018 <400> 8 actcagctgg gattcttcct gc 22 <210> 9 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK01_018 <400> 9 gaagctcact tctcttaacc tttcatcaa 29 <210> 10 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK01_025 <400> 10 gaataccatt tgtaacggat ttatatacga 30 <210> 11 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK01_025 <400> 11 aataccattt gtaacggatt tatatacgg 29 <210> 12 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK01_025 <400> 12 gtagttatcc ctaacataaa tgacatcgta 30 <210> 13 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK01_030 <400> 13 atcgtacaac catccaacca gtac 24 <210> 14 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK01_030 <400> 14 gatcgtacaa ccatccaacc agtaa 25 <210> 15 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK01_030 <400> 15 gggaggttaa tgagctaggt cgtta 25 <210> 16 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK01_038 <400> 16 actgcatatt tgctatgtta ctactgc 27 <210> 17 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK01_038 <400> 17 gactgcatat ttgctatgtt actactgt 28 <210> 18 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK01_038 <400> 18 gtggtatgat gtactcatgg ttgttgatt 29 <210> 19 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK01_046 <400> 19 tgttacaggc accagtaaag cc 22 <210> 20 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK01_046 <400> 20 gttgttacag gcaccagtaa agct 24 <210> 21 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK01_046 <400> 21 taaatggaag gggacgtgcg agtt 24 <210> 22 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK01_054 <400> 22 atcaatttgg gtggactggt tgtac 25 <210> 23 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK01_054 <400> 23 catcaatttg ggtggactgg ttgtat 26 <210> 24 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK01_054 <400> 24 gtccaaacat aggtgttttg taaccgatt 29 <210> 25 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK01_070 <400> 25 ctttgctagt gttataccag gacc 24 <210> 26 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK01_070 <400> 26 atctttgcta gtgttatacc aggact 26 <210> 27 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK01_070 <400> 27 catcaacaaa tacggtgaga catctacaa 29 <210> 28 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK01_084 <400> 28 ttttcgtcga ttctgagacg agg 23 <210> 29 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK01_084 <400> 29 tttcgtcgat tctgagacga gc 22 <210> 30 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK01_084 <400> 30 aactcgatgc aagaatcagc gaattcaaa 29 <210> 31 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK01_093 <400> 31 tcagggatgt gtgaagggag c 21 <210> 32 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK01_093 <400> 32 tcagggatgt gtgaagggag t 21 <210> 33 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK01_093 <400> 33 agcaatacaa aaaccagaag cacctctt 28 <210> 34 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK01_102 <400> 34 acctttgtgc aacttttgtt ttagttttg 29 <210> 35 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK01_102 <400> 35 gacctttgtg caacttttgt tttagtttta 30 <210> 36 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK01_102 <400> 36 ctcagacaag ccttttgtgc atataagtt 29 <210> 37 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK01_109 <400> 37 gttgatcatc aatctatgca gacact 26 <210> 38 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK01_109 <400> 38 gttgatcatc aatctatgca gacacc 26 <210> 39 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK01_109 <400> 39 cccctcttgt gagaagagtt agattttaa 29 <210> 40 <211> 20 <212> DNA <213> artificial sequence <220> <223> SK01_113 <400> 40 caacccaacg gactgacccg 20 <210> 41 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK01_113 <400> 41 acaacccaac ggactgaccc a 21 <210> 42 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK01_113 <400> 42 aagaaaaagg gtttctgttt tcgtgagaat 30 <210> 43 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK01_120 <400> 43 aataatgttg aaactagtat gatcaatagt a 31 <210> 44 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK01_120 <400> 44 aataatgttg aaactagtat gatcaatagt g 31 <210> 45 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK01_120 <400> 45 cagtttccag gcttgttgaa attggtata 29 <210> 46 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK01_128 <400> 46 ggggagtgaag tcatcgtgtc gtt 23 <210> 47 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK01_128 <400> 47 ggaggtgaagt catcgtgtcg tc 22 <210> 48 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK01_128 <400> 48 agcagcagat gaggactccc aatt 24 <210> 49 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK01_133 <400> 49 ggcttatgct tgcacagccg ata 23 <210> 50 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK01_133 <400> 50 gcttatgctt gcacagccga tg 22 <210> 51 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK01_133 <400> 51 gctattgaaa atctcaagaa ctccagcaa 29 <210> 52 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK01_139 <400> 52 gtccgatttt tagttgaggt atcttac 27 <210> 53 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK01_139 <400> 53 ggtccgattt ttagttgagg tatcttat 28 <210> 54 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK01_139 <400> 54 gatcttacag agaacttctg agtaaatctt 30 <210> 55 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK01_145 <400> 55 agtggtttaa ctctccaggt gtac 24 <210> 56 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK01_145 <400> 56 cagtggttta actctccagg tgtat 25 <210> 57 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK01_145 <400> 57 cggatagggc ggagaaagga taata 25 <210> 58 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK01_152 <400> 58 gagatacttg tatatttatg ttaacacaag g 31 <210> 59 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK01_152 <400> 59 ggagatactt gtattattat gttaacacaa ga 32 <210> 60 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK01_152 <400> 60 gctttagtta aaaatgggat aggggactt 29 <210> 61 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK01_160 <400> 61 ccaggtctat taattaggtt catcttac 28 <210> 62 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK01_160 <400> 62 aaccaggtct attaattagg ttcatcttat 30 <210> 63 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK01_160 <400> 63 tattgatctc aaaatccatc tcgatcagat 30 <210> 64 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK01_174 <400> 64 attgctgcat acgggaaagg tttc 24 <210> 65 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK01_174 <400> 65 gctgcatacg ggaaaggttt t 21 <210> 66 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK01_174 <400> 66 cccaagtgtc ttgggacgcc tt 22 <210> 67 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK01_181 <400> 67 tcttaactac ctgcaaaacg ca 22 <210> 68 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK01_181 <400> 68 cttcttaact acctgcaaaa cgcg 24 <210> 69 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK01_181 <400> 69 gtacaaacaa gagaaccaag atggtgttt 29 <210> 70 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK01_187 <400> 70 aaggtaccag aattcttgct tgcga 25 <210> 71 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK01_187 <400> 71 ggtaccagaa ttcttgcttg cgc 23 <210> 72 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK01_187 <400> 72 gatggatgtt ttccgcaaga atgattcaa 29 <210> 73 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK01_194 <400> 73 atcagagaaa atagacacta agctggt 27 <210> 74 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK01_194 <400> 74 cagagaaaat agacactaag ctggc 25 <210> 75 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK01_194 <400> 75 gaaatgcttg tggaggtcat cttgtaatt 29 <210> 76 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK01_201 <400> 76 gattataaat catgcatcct tgtattttga tta 33 <210> 77 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK01_201 <400> 77 ataaatcatg catccttgta ttttgattt 29 <210> 78 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK01_201 <400> 78 gtggcaacaa agtcgatcta aaaagcttt 29 <210> 79 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK02_007 <400> 79 gaaatgatca aaatacccat attgagac 28 <210> 80 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK02_007 <400> 80 gtgaaatgat caaaataccc atattgagat 30 <210> 81 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK02_007 <400> 81 ggccaataca agcatgattt tgatgagat 29 <210> 82 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK02_013 <400> 82 gatctccagg atggggggtt g 21 <210> 83 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK02_013 <400> 83 gatctccagg atggggggtt c 21 <210> 84 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK02_013 <400> 84 actggaacga ttattaatga agatacgctt 30 <210> 85 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK02_022 <400> 85 gtggttagaa ataaaggtgc tcccat 26 <210> 86 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK02_022 <400> 86 ggttagaaat aaaggtgctc ccag 24 <210> 87 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK02_022 <400> 87 atctcttgct tagagtaggt accctaat 28 <210> 88 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK02_027 <400> 88 gtattggcag tatatgccat tgaagt 26 <210> 89 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK02_027 <400> 89 gtattggcag tatatgccat tgaagg 26 <210> 90 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK02_027 <400> 90 acgagcttct cgacaaatag taacttattt 30 <210> 91 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK02_036 <400> 91 gaaattatgc agcagcatca catgtc 26 <210> 92 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK02_036 <400> 92 agaaattatg cagcagcatc acatgtt 27 <210> 93 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK02_036 <400> 93 gtgcctattt tgtttatatg aaagcattaa 30 <210> 94 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK02_041 <400> 94 gtaatttgag tagaatacca accattgg 28 <210> 95 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK02_041 <400> 95 gtaatttgag tagaatacca accattgc 28 <210> 96 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK02_041 <400> 96 ttgccgatgt ggatcaagtt tgatactat 29 <210> 97 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK02_050 <400> 97 ttgtgattag tttctacttc gaccc 25 <210> 98 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK02_050 <400> 98 gtttgtgatt agtttctact tcgacct 27 <210> 99 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK02_050 <400> 99 aaacctaaac aacagaaaaa gagcatgcaa 30 <210> 100 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK02_059 <400> 100 ccattacaaa gtcgttgcaa agac 24 <210> 101 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK02_059 <400> 101 actccattac aaagtcgttg caaagat 27 <210> 102 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK02_059 <400> 102 ggcattttct gaacaaccaa atagccatt 29 <210> 103 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK02_070 <400> 103 agattacaaa gcagaaaacg gaaagtg 27 <210> 104 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK02_070 <400> 104 caagattaca aagcagaaaa cggaaagta 29 <210> 105 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK02_070 <400> 105 gctgatgatg aatgagtaac ggaaagatt 29 <210> 106 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK02_076 <400> 106 gattagaagt ggtttgctct ccac 24 <210> 107 <211> 20 <212> DNA <213> artificial sequence <220> <223> SK02_076 <400> 107 agaagtggtt tgctctccag 20 <210> 108 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK02_076 <400> 108 cagcaaaaaa gatcagaaat cagccttcaa 30 <210> 109 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK02_081 <400> 109 acaatcaaca actactatta aagtaaaata aca 33 <210> 110 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK02_081 <400> 110 acaatcaaca actactatta aagtaaaata acg 33 <210> 111 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK02_081 <400> 111 ggcttttcga atcccactaa cgtgtt 26 <210> 112 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK02_087 <400> 112 attaaacatc ctgaaatgta aaatctaaga tga 33 <210> 113 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK02_087 <400> 113 aaacatcctg aaatgtaaaa tctaagatgc 30 <210> 114 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK02_087 <400> 114 tgcatctgaa tgcaaaggca tgaaaagat 29 <210> 115 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK02_089 <400> 115 ttttgttgtt ctttgcattt atttttatcc ag 32 <210> 116 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK02_089 <400> 116 tttgttgttc tttgcattta tttttatcca a 31 <210> 117 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK02_089 <400> 117 acaaaaatag agtagatggg aggtctgaa 29 <210> 118 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK02_097 <400> 118 tcacttgtaa ttttgttgat tccg 24 <210> 119 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK02_097 <400> 119 ctgcttcact tgtaattttg ttgattcca 29 <210> 120 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK02_097 <400> 120 ggcatataaa caacccaatg tcctttcta 29 <210> 121 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK02_103 <400> 121 caagtacatc caacataatt ggaatgc 27 <210> 122 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK02_103 <400> 122 ccaagtacat ccaacataat tggaatgt 28 <210> 123 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK02_103 <400> 123 gtagagggtc aagttgatga taatcatgaa 30 <210> 124 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK02_110 <400> 124 ccgtcgacac aatagtttcg gg 22 <210> 125 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK02_110 <400> 125 ccgtcgacac aatagtttcg gc 22 <210> 126 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK02_110 <400> 126 cgtagcattc tgcaccagcc caa 23 <210> 127 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK02_117 <400> 127 ggcatcacaa atcatcgatg tcacat 26 <210> 128 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK02_117 <400> 128 ggcatcacaa atcatcgatg tcacaa 26 <210> 129 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK02_117 <400> 129 ttgagctgcc cagaatcatt aacaagaaa 29 <210> 130 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK02_121 <400> 130 atgtgttggc ttgaaaagat taaaagtca 29 <210> 131 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK02_121 <400> 131 gtgttggctt gaaaagatta aaagtcg 27 <210> 132 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK02_121 <400> 132 ctcctctctt tagcatcttt agttctagtt 30 <210> 133 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK02_133 <400> 133 tttgtttgag gacattggag gtg 23 <210> 134 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK02_133 <400> 134 cttttgtttg aggacattgg aggtc 25 <210> 135 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK02_133 <400> 135 ggcattagca gccaatggga tattaaatt 29 <210> 136 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK02_140 <400> 136 ccatgaatca aagcaactcg ccg 23 <210> 137 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK02_140 <400> 137 aaccatgaat caaagcaact cgcca 25 <210> 138 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK02_140 <400> 138 aagatttaac cagttgatgc aaaactggat 30 <210> 139 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK02_151 <400> 139 gacttttgca tatatgagaa tgtgtcttt 29 <210> 140 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK02_151 <400> 140 gacttttgca tatatgagaa tgtgtcttc 29 <210> 141 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK02_151 <400> 141 gtgggaaggac atgtttgcag tatagtata 29 <210> 142 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK02_160 <400> 142 attcttgagg tggtcctgca catat 25 <210> 143 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK02_160 <400> 143 cttgaggtgg tcctgcacat ac 22 <210> 144 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK02_160 <400> 144 ttgaacttat ggaacaaagt gcggacaaa 29 <210> 145 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK02_167 <400> 145 cattttgttt tactacagta aattccaata ata 33 <210> 146 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK02_167 <400> 146 cattttgttt tactacagta aattccaata atg 33 <210> 147 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK02_167 <400> 147 ggtgtacaag gagatgaggc atcat 25 <210> 148 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK02_179 <400> 148 atgaattcac ggagccacgc g 21 <210> 149 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK02_179 <400> 149 catgaattca cggagccacg ca 22 <210> 150 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK02_179 <400> 150 cagacttcag tgataggcct cgtt 24 <210> 151 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK03_003 <400> 151 gttttctgtc tccgttactc ctctt 25 <210> 152 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK03_003 <400> 152 ttctgtctcc gttactcctc tc 22 <210> 153 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK03_003 <400> 153 ttgagcaggc gaacaagaga ctgat 25 <210> 154 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK03_012 <400> 154 ggtctgatca gaaacctgtc gac 23 <210> 155 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK03_012 <400> 155 ggtctgatca gaaacctgtc gag 23 <210> 156 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK03_012 <400> 156 cagctttgga aacccattaa agattgcat 29 <210> 157 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK03_020 <400> 157 aaagattgcg gtgatgtgca aac 23 <210> 158 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK03_020 <400> 158 ctaaagatg cggtgatgtg caaag 25 <210> 159 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK03_020 <400> 159 catgaactgg gaggtttcaa tctttagtt 29 <210> 160 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK03_029 <400> 160 gaggtagatt ttatatttca ggtaggg 27 <210> 161 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK03_029 <400> 161 ggaggtagat tttatatttc aggtagga 28 <210> 162 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK03_029 <400> 162 tagagcgaaa tccaacctta tgttccaaa 29 <210> 163 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK03_030 <400> 163 ggtaaacatg gtctttaagt gggc 24 <210> 164 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK03_030 <400> 164 ggtaaacatg gtctttaagt ggga 24 <210> 165 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK03_030 <400> 165 tgtcagggtt gtcaaaaaac aagcacaaa 29 <210> 166 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK03_038 <400> 166 gagcctggtg tactaggttt tcc 23 <210> 167 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK03_038 <400> 167 gagcctggtg tactaggttt tct 23 <210> 168 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK03_038 <400> 168 ccactgaaaa attactaggt taagcccaa 29 <210> 169 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK03_044 <400> 169 tgttttagga agttgaattc aagttgg 27 <210> 170 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK03_044 <400> 170 cttgttttag gaagttgaat tcaagttgc 29 <210> 171 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK03_044 <400> 171 ccttacaaat ttggttttga agcttcagtt 30 <210> 172 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK03_050 <400> 172 aagatgggtg aaggtattcg acct 24 <210> 173 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK03_050 <400> 173 gatgggtgaa ggtattcgac cc 22 <210> 174 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK03_050 <400> 174 gtgttgcgga aatgtttcaa aggattgat 29 <210> 175 <211> 20 <212> DNA <213> artificial sequence <220> <223> SK03_055 <400> 175 gtttcgaagt gggctgtgag 20 <210> 176 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK03_055 <400> 176 ctgtttcgaa gtgggctgtg ac 22 <210> 177 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK03_055 <400> 177 gtaagtcttg tggtatagtg ttaagcagat 30 <210> 178 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK03_063 <400> 178 gcaagctcag tgatgtgact acaaa 25 <210> 179 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK03_063 <400> 179 caagctcagt gatgtgacta caag 24 <210> 180 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK03_063 <400> 180 gaacatttca tatgcaggca gagtacaaa 29 <210> 181 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK03_070 <400> 181 cagccattct tgtttttctt ctggc 25 <210> 182 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK03_070 <400> 182 cagccattct tgtttttctt ctggt 25 <210> 183 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK03_070 <400> 183 tacaatggtc ccacgagtat taatccaaa 29 <210> 184 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK03_079 <400> 184 gtaaatacat gatcagccta ctggg 25 <210> 185 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK03_079 <400> 185 ggtaaataca tgatcagcct actgga 26 <210> 186 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK03_079 <400> 186 gccgaaggcc atatttctca gcata 25 <210> 187 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK03_094 <400> 187 ataacagtga ttaaatccat accagatatc 30 <210> 188 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK03_094 <400> 188 gataacagtg attaaatcca taccagatat t 31 <210> 189 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK03_094 <400> 189 cccgtcactt tctactacaa tgtaatgat 29 <210> 190 <211> 20 <212> DNA <213> artificial sequence <220> <223> SK03_098 <400> 190 ttgccaagac agttgcggac 20 <210> 191 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK03_098 <400> 191 ctttgccaag acagttgcgg ag 22 <210> 192 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK03_098 <400> 192 gctctcgaac aacagtctta ctgcaa 26 <210> 193 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK03_102 <400> 193 cccagaaaga atttgcatgc aaattcat 28 <210> 194 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK03_102 <400> 194 ccagaaagaa tttgcatgca aattcac 27 <210> 195 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK03_102 <400> 195 aatgtaagca tactactcat cctggtctt 29 <210> 196 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK03_108 <400> 196 ggatgaaata taggaagatg acctttg 27 <210> 197 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK03_108 <400> 197 gggatgaaat ataggaagat gacctttt 28 <210> 198 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK03_108 <400> 198 gacggaccca actaatttac atgacaatt 29 <210> 199 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK03_116 <400> 199 agatctaaag taacttctaa gtaaaaaatt gag 33 <210> 200 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK03_116 <400> 200 agatctaaag taacttctaa gtaaaaaatt gat 33 <210> 201 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK03_116 <400> 201 gagtattctg ggtcaaacac ttggatatt 29 <210> 202 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK03_120 <400> 202 atccgaagat gaactgatga tgagat 26 <210> 203 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK03_120 <400> 203 ccgaagatga actgatgatg agac 24 <210> 204 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK03_120 <400> 204 gtgatgtgtg ccggcaatca tcat 24 <210> 205 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK03_126 <400> 205 agtttctctc agagaccatg tttgg 25 <210> 206 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK03_126 <400> 206 agtttctctc agagaccatg tttgc 25 <210> 207 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK03_126 <400> 207 actcacaaac caaggaaagt gagatcaaa 29 <210> 208 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK03_141 <400> 208 attttctgg aaccagagaa gactgta 27 <210> 209 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK03_141 <400> 209 tttctggaac cagagaagac tgtt 24 <210> 210 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK03_141 <400> 210 catgtcgtgt gtttgtgtaa gtgtaactt 29 <210> 211 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK03_147 <400> 211 attaaaaaat catttgccgc aactaagagt 30 <210> 212 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK03_147 <400> 212 aaaaaatcat ttgccgcaac taagagg 27 <210> 213 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK03_147 <400> 213 gccgtggttt taatgagaat ggctaaaat 29 <210> 214 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK03_151 <400> 214 gaaagtgtat taacaaacca gaaatagcc 29 <210> 215 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK03_151 <400> 215 agaaagtgta ttaacaaacc agaaatagct 30 <210> 216 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK03_151 <400> 216 gcgcttgcat gatattaagt gatgcaatt 29 <210> 217 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK03_158 <400> 217 atataagtta tagcgtttta tacaatgtaa t 31 <210> 218 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK03_158 <400> 218 ctatataagt tatagcgttt tatacaatgt aac 33 <210> 219 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK03_158 <400> 219 tgtacgattg aagggtgcaa agtgttatt 29 <210> 220 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK03_166 <400> 220 acactagata attggacggt atttcac 27 <210> 221 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK03_166 <400> 221 acactagata attggacggt atttcag 27 <210> 222 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK03_166 <400> 222 acatggcagt cccattatga ataaccaaa 29 <210> 223 <211> 20 <212> DNA <213> artificial sequence <220> <223> SK03_173 <400> 223 cttactttgg ggcaggtgca 20 <210> 224 <211> 20 <212> DNA <213> artificial sequence <220> <223> SK03_173 <400> 224 cttactttgg ggcaggtgcg 20 <210> 225 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK03_173 <400> 225 aaaggcggaa aatccattga tcatgagaa 29 <210> 226 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK03_180 <400> 226 gtaattaatt tatatctttt gttgttactt tta 33 <210> 227 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK03_180 <400> 227 gtaattaatt tatatctttt gttgttactt ttg 33 <210> 228 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK03_180 <400> 228 gcatagaatg ccataaaata aaaagttcaa 30 <210> 229 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK03_191 <400> 229 gatgtatatg ttagtaatac acatttattt ac 32 <210> 230 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK03_191 <400> 230 gatgtatatg ttagtaatac acatttattt at 32 <210> 231 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK03_191 <400> 231 gtagaatgta tgtgtccatt atgtggctt 29 <210> 232 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK03_203 <400> 232 ccttatcacc accccctgga a 21 <210> 233 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK03_203 <400> 233 ccttatcacc accccctgga t 21 <210> 234 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK03_203 <400> 234 gtcggagagg cagctgctga tt 22 <210> 235 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK03_219 <400> 235 aaatcattat attttattta tgtacacaca ttg 33 <210> 236 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK03_219 <400> 236 aaatcattat attttattta tgtacacaca ttt 33 <210> 237 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK03_219 <400> 237 tttagttttt attagtgatg gcggcacatt 30 <210> 238 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK03_226 <400> 238 gacatttaaa ccaaatatgt caattgtatt ta 32 <210> 239 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK03_226 <400> 239 gacatttaaa ccaaatatgt caattgtatt tc 32 <210> 240 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK03_226 <400> 240 ctgacgtagc acgaggaaca taagaa 26 <210> 241 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK03_237 <400> 241 gatttggcga agtgaagtgc gaaa 24 <210> 242 <211> 20 <212> DNA <213> artificial sequence <220> <223> SK03_237 <400> 242 tggcgaagtg aagtgcgaat 20 <210> 243 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK03_237 <400> 243 gtcgccctgt tcattcacct caatt 25 <210> 244 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK03_245 <400> 244 cgttcacaat tatatggaga ataccaac 28 <210> 245 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK03_245 <400> 245 ccgttcacaa ttatatggag aataccaat 29 <210> 246 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK03_245 <400> 246 tcgaatgtcg tgatatgata atatttcttt 30 <210> 247 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK03_252 <400> 247 aactccgcca taggctccat ct 22 <210> 248 <211> 20 <212> DNA <213> artificial sequence <220> <223> SK03_252 <400> 248 ctccgccata ggctccatcg 20 <210> 249 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK03_252 <400> 249 tggcctcgca attccaggaa agtta 25 <210> 250 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK03_258 <400> 250 catatttatt agcatttttt gttctctaac a 31 <210> 251 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK03_258 <400> 251 ctcatattta ttagcatttt ttgttctcta act 33 <210> 252 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK03_258 <400> 252 atttgcatga cgaaaatgcc accttagaa 29 <210> 253 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK04_008 <400> 253 aaatatgaca gactgctgcc gt 22 <210> 254 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK04_008 <400> 254 aaatatgaca gactgctgcc gc 22 <210> 255 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK04_008 <400> 255 ccatctcatg gaccgtgttt tccat 25 <210> 256 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK04_015 <400> 256 aggatggaga tccgtgccta tttaa 25 <210> 257 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK04_015 <400> 257 aggatggaga tccgtgccta tttat 25 <210> 258 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK04_015 <400> 258 cccatgtgca atgaatatat tcgaacgat 29 <210> 259 <211> 19 <212> DNA <213> artificial sequence <220> <223> SK04_023 <400> 259 ggcaagtgca gcctctccg 19 <210> 260 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK04_023 <400> 260 gtggcaagtg cagcctctcc a 21 <210> 261 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK04_023 <400> 261 agtggcagag tacttgaatt gtcactaaa 29 <210> 262 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK04_029 <400> 262 ccaagaaaga attaggagag gaagat 26 <210> 263 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK04_029 <400> 263 ccaagaaaga attaggagag gaagac 26 <210> 264 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK04_029 <400> 264 atgtgcttcc caatttgttg aagttggat 29 <210> 265 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK04_045 <400> 265 ccattcttga gaccatgaat gagtat 26 <210> 266 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK04_045 <400> 266 ccattcttga gaccatgaat gagtac 26 <210> 267 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK04_045 <400> 267 acaggacctc aaaattgtca tgatggtta 29 <210> 268 <211> 18 <212> DNA <213> artificial sequence <220> <223> SK04_051 <400> 268 gtcggccctc cactcacc 18 <210> 269 <211> 18 <212> DNA <213> artificial sequence <220> <223> SK04_051 <400> 269 gtcggccctc cactcact 18 <210> 270 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK04_051 <400> 270 gacgaagagg aaatgaacac cataaaagat 30 <210> 271 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK04_060 <400> 271 gtaaagatgc tggtgaaagg agg 23 <210> 272 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK04_060 <400> 272 ggtaaagatg ctggtgaaag gaga 24 <210> 273 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK04_060 <400> 273 cggcgaacat ccaccttctt aaagaa 26 <210> 274 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK04_069 <400> 274 atctgtagtc attggcgtct gag 23 <210> 275 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK04_069 <400> 275 catctgtagt cattggcgtc tgat 24 <210> 276 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK04_069 <400> 276 caagacgggg atgaattggt agtcta 26 <210> 277 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK04_080 <400> 277 gtagtactaa ttaaaattct acccacagat t 31 <210> 278 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK04_080 <400> 278 agtactaatt aaaattctac ccacagatc 29 <210> 279 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK04_080 <400> 279 ttccacattt cctattgttc actttcccat 30 <210> 280 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK04_090 <400> 280 tcagactgtg atcagcatct ttcg 24 <210> 281 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK04_090 <400> 281 gttcagactg tgatcagcat ctttct 26 <210> 282 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK04_090 <400> 282 cagctgatca gcagtttctt atctatgat 29 <210> 283 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK04_098 <400> 283 gataaggagg taatattgca tgctatct 28 <210> 284 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK04_098 <400> 284 ataaggaggt aatattgcat gctatcc 27 <210> 285 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK04_098 <400> 285 gagttcaaga aaacaagaaa ctctgaagta 30 <210> 286 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK04_107 <400> 286 tgttatcatg tccaactaaa taaataaaat cc 32 <210> 287 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK04_107 <400> 287 gttatcatgt ccaactaaat aaataaaatc t 31 <210> 288 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK04_107 <400> 288 ggctccgttt attttgtgaa atgtcattaa 30 <210> 289 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK04_114 <400> 289 catggtcttg cgcgaagtac tg 22 <210> 290 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK04_114 <400> 290 gcatggtctt gcgcgaagta cta 23 <210> 291 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK04_114 <400> 291 gcctttctca caagagactt cagcat 26 <210> 292 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK04_122 <400> 292 cattgtatta tgtcggcctg catac 25 <210> 293 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK04_122 <400> 293 cattgtatta tgtcggcctg catag 25 <210> 294 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK04_122 <400> 294 cgccagataa agatgcctct ccata 25 <210> 295 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK04_129 <400> 295 aaaagaaaac agacatgcca cttgaac 27 <210> 296 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK04_129 <400> 296 aaaagaaaac agacatgcca cttgaag 27 <210> 297 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK04_129 <400> 297 ggctatcata tactcacctt ttcggttaa 29 <210> 298 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK04_138 <400> 298 gataacatgt tcgaaaatga acatagtata aa 32 <210> 299 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK04_138 <400> 299 gataacatgt tcgaaaatga acatagtata at 32 <210> 300 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK04_138 <400> 300 tcccacaaaa agtaggcatc atgacttat 29 <210> 301 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK04_143 <400> 301 tgcgttctca cctactctcc g 21 <210> 302 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK04_143 <400> 302 cttgcgttct cacctactct cct 23 <210> 303 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK04_143 <400> 303 gttccacttg acggaattca cacgta 26 <210> 304 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK04_149 <400> 304 ccaatactag ccctactaat tttaactg 28 <210> 305 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK04_149 <400> 305 ccaatactag ccctactaat tttaacta 28 <210> 306 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK04_149 <400> 306 agttggctgc attaatgcag taactgtt 28 <210> 307 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK04_158 <400> 307 actgttaact attctgcatg ggtttct 27 <210> 308 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK04_158 <400> 308 ctgttaacta ttctgcatgg gtttcc 26 <210> 309 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK04_158 <400> 309 caccccgtct tgtgttggtt tagaa 25 <210> 310 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK04_164 <400> 310 gcaattaaga gatcctgcaa accaat 26 <210> 311 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK04_164 <400> 311 gcaattaaga gatcctgcaa accaag 26 <210> 312 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK04_164 <400> 312 ctgagtacca ttactgctag tttcttctt 29 <210> 313 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK04_174 <400> 313 attatacata taccatgttg acttcaaaat tat 33 <210> 314 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK04_174 <400> 314 atacatatac catgttgact tcaaaattac 30 <210> 315 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK04_174 <400> 315 aaatgtggtt tcgtctattt atggtgagta 30 <210> 316 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK04_180 <400> 316 aaaagtttca actaaaaatg agttcctagc 30 <210> 317 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK04_180 <400> 317 aaaaaaagtt tcaactaaaa atgagttcct aga 33 <210> 318 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK04_180 <400> 318 ctggtctgag gtatgcctat taccaa 26 <210> 319 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK04_183 <400> 319 cttgcaggta tcgctaatat gacc 24 <210> 320 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK04_183 <400> 320 ctcttgcagg tatcgctaat atgact 26 <210> 321 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK04_183 <400> 321 gcgcgaagaa atcaatagga ggacaa 26 <210> 322 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK04_189 <400> 322 ccagccatcc agaacagtct ca 22 <210> 323 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK04_189 <400> 323 cagccatcca gaacagtctc g 21 <210> 324 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK04_189 <400> 324 gttatgtggg actccgaagt cttcaa 26 <210> 325 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK04_195 <400> 325 attatttattttcacatatc atgaattaat ca 32 <210> 326 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK04_195 <400> 326 attatttattttcacatatc atgaattaat cc 32 <210> 327 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK04_195 <400> 327 gagcccaata agacaactgg ctcaa 25 <210> 328 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK04_200 <400> 328 agagcaagag atggatttgt ggac 24 <210> 329 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK04_200 <400> 329 gagagcaaga gatggatttg tggat 25 <210> 330 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK04_200 <400> 330 ctcaaaagca tcctttcaca tacagcaaa 29 <210> 331 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK04_204 <400> 331 acgtgaaact gaagaaatta aggcg 25 <210> 332 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK04_204 <400> 332 ctacgtgaaa ctgaagaaat taaggca 27 <210> 333 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK04_204 <400> 333 ggctgtgaat gagaaatctg agcctt 26 <210> 334 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK05_003 <400> 334 gaatgctttt gtaagaagac cgtggt 26 <210> 335 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK05_003 <400> 335 aatgcttttg taagaagacc gtggc 25 <210> 336 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK05_003 <400> 336 gaagactcaa atctgtggggc aggaa 25 <210> 337 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK05_010 <400> 337 actgtgtttc attgacggaa cttcc 25 <210> 338 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK05_010 <400> 338 aaactgtgtt tcattgacgg aacttct 27 <210> 339 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK05_010 <400> 339 gaagtgtgga tcattagcgt tcggtt 26 <210> 340 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK05_015 <400> 340 tggtaaactt tcgagttctg ttatatttg 29 <210> 341 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK05_015 <400> 341 ggtaaacttt cgagttctgt tatatttc 28 <210> 342 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK05_015 <400> 342 gagtctagct cgagcagtat aatacttt 28 <210> 343 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK05_017 <400> 343 ggtctaagta gttgctagtc tgcta 25 <210> 344 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK05_017 <400> 344 ggtctaagta gttgctagtc tgctt 25 <210> 345 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK05_017 <400> 345 gtgggaacat aaagaccata ggctcat 27 <210> 346 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK05_032 <400> 346 tctttctgaa gagtaagcaa tgcatc 26 <210> 347 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK05_032 <400> 347 atattctttc tgaagagtaa gcaatgcata 30 <210> 348 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK05_032 <400> 348 gctcgccaga agacaggaaa tacta 25 <210> 349 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK05_036 <400> 349 atctacaatg gcgaacgatg ctgat 25 <210> 350 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK05_036 <400> 350 ctacaatggc gaacgatgct gac 23 <210> 351 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK05_036 <400> 351 cagaagatca gtccatccgc tcat 24 <210> 352 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK05_039 <400> 352 aaggaaagaa cattgcacta gcaaaa 26 <210> 353 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK05_039 <400> 353 aaggaaagaa cattgcacta gcaaag 26 <210> 354 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK05_039 <400> 354 cactgtatta cgttctatgt cgagctat 28 <210> 355 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK05_059 <400> 355 acagctccat ttggatgatt cgg 23 <210> 356 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK05_059 <400> 356 agacagctcc atttggatga ttcga 25 <210> 357 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK05_059 <400> 357 cgccaacccc attagcctcc tt 22 <210> 358 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK05_068 <400> 358 aacattacat gccaaatttg tgcg 24 <210> 359 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK05_068 <400> 359 cctaacatta catgccaaat ttgtgca 27 <210> 360 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK05_068 <400> 360 gtcaaattgg attattacta cccaacacaa 30 <210> 361 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK05_075 <400> 361 ggcatatttt aaatgaaaat ggatctgatt g 31 <210> 362 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK05_075 <400> 362 aggcatattt taaatgaaaa tggatctgat ta 32 <210> 363 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK05_075 <400> 363 aaactcagca attctacgag gacattctt 29 <210> 364 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK05_087 <400> 364 cgagacaatt ctatgtattt aattatttat gg 32 <210> 365 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK05_087 <400> 365 cgagacaatt ctatgtattt aattatttat gc 32 <210> 366 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK05_087 <400> 366 cctaattcac atttcttatg atggggtctt 30 <210> 367 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK05_092 <400> 367 aacaatttcc tcggtttat ccattatgta 30 <210> 368 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK05_092 <400> 368 aacaatttcc tcggtttat ccattatgtt 30 <210> 369 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK05_092 <400> 369 gtgtttgtct gaaaccccat ttcatagat 29 <210> 370 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK05_100 <400> 370 aaagaaaatt ttttcatgaa cgtaatttca ctc 33 <210> 371 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK05_100 <400> 371 aaagaaaatt ttttcatgaa cgtaatttca ctt 33 <210> 372 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK05_100 <400> 372 gattctattc ccataatcga aaattagaaa 30 <210> 373 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK05_108 <400> 373 aacaaatgat atcagagttg accacc 26 <210> 374 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK05_108 <400> 374 gtaacaaatg atatcagagt tgaccact 28 <210> 375 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK05_108 <400> 375 catatcactt agttccctac tctgctt 27 <210> 376 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK05_112 <400> 376 gtcaaatagt ccttaggatt tgcaac 26 <210> 377 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK05_112 <400> 377 aatgtcaaat agtccttagg atttgcaat 29 <210> 378 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK05_112 <400> 378 tagttgaagc ccttaaagca aagggaata 29 <210> 379 <211> 17 <212> DNA <213> artificial sequence <220> <223> SK05_115 <400> 379 agtcgggttc gtccccg 17 <210> 380 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK05_115 <400> 380 aaactagtcg ggttcgtccc ca 22 <210> 381 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK05_115 <400> 381 ccaattaact taagcatcag agggtcatt 29 <210> 382 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK05_122 <400> 382 aaggcattaa catactatct caactttaat aa 32 <210> 383 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK05_122 <400> 383 ggcattaaca tactatctca actttaatag 30 <210> 384 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK05_122 <400> 384 gtgataacgt ggaacttaat ttaccttaat 30 <210> 385 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK05_132 <400> 385 caatagttgg taaataaatt cacgagtttc t 31 <210> 386 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK05_132 <400> 386 aatagttggt aaataaattc acgagtttcc 30 <210> 387 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK05_132 <400> 387 cttgggtggt gaaggaagag gttt 24 <210> 388 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK05_137 <400> 388 acagaccttc atcgtccatt aggt 24 <210> 389 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK05_137 <400> 389 cagaccttca tcgtccatta ggc 23 <210> 390 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK05_137 <400> 390 ataactgcac ggacaggcaa accat 25 <210> 391 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK05_148 <400> 391 attcatcata taaaagagca aaaactgcg 29 <210> 392 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK05_148 <400> 392 cattcatcat ataaaagagc aaaaactgca 30 <210> 393 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK05_148 <400> 393 caaactgaca gacaccaaat gaagcaaat 29 <210> 394 <211> 19 <212> DNA <213> artificial sequence <220> <223> SK05_155 <400> 394 acaggcagtg gacctgccg 19 <210> 395 <211> 19 <212> DNA <213> artificial sequence <220> <223> SK05_155 <400> 395 acaggcagtg gacctgccc 19 <210> 396 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK05_155 <400> 396 ctcaccacag accggaccca tt 22 <210> 397 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK05_162 <400> 397 aaatatttct atttagcatg cttgttgaaa t 31 <210> 398 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK05_162 <400> 398 aaatatttct atttagcatg cttgttgaaa c 31 <210> 399 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK05_162 <400> 399 gaactccgtt agctgaactc cacat 25 <210> 400 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK06_006 <400> 400 cccgtaccat caaaattaaa ttatattgt tc 32 <210> 401 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK06_006 <400> 401 cccgtaccat caaaattaaa ttattatgt ta 32 <210> 402 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK06_006 <400> 402 cgtgctaatt ttatccataa tgtcaaccaa 30 <210> 403 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK06_013 <400> 403 aactcattta aacaagatag tcttaggca 29 <210> 404 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK06_013 <400> 404 aactcattta aacaagatag tcttaggct 29 <210> 405 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK06_013 <400> 405 ctttaatctt tcttttttgc tcacggctaa 30 <210> 406 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK06_022 <400> 406 tgtgagcgct aaatttattg tatctg 26 <210> 407 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK06_022 <400> 407 aacttgtgag cgctaaattt attgtatcta 30 <210> 408 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK06_022 <400> 408 cccaatactt ttatttgtga taacttattt 30 <210> 409 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK06_029 <400> 409 cactcataag tcaaaaccca ctca 24 <210> 410 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK06_029 <400> 410 cactcataag tcaaaccca ctcc 24 <210> 411 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK06_029 <400> 411 gtctccttta gccccaaact ccatt 25 <210> 412 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK06_035 <400> 412 ataccaaata ttctattctg atactaacaa tac 33 <210> 413 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK06_035 <400> 413 ataccaaata ttctattctg atactaacaa taa 33 <210> 414 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK06_035 <400> 414 ccaagaccaa gcattgattt gatgctata 29 <210> 415 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK06_042 <400> 415 cataactatc tcgtgaagtg gcg 23 <210> 416 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK06_042 <400> 416 ccataactat ctcgtgaagt ggca 24 <210> 417 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK06_042 <400> 417 cctggtttga gacactaagg aaacaaata 29 <210> 418 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK06_048 <400> 418 attattccat caatccagca tatgttctt 29 <210> 419 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK06_048 <400> 419 attccatcaa tccagcatat gttctc 26 <210> 420 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK06_048 <400> 420 gtacgttaac tgagtatcaa agggagaaa 29 <210> 421 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK06_056 <400> 421 gtttgtagca ccacttcgac cg 22 <210> 422 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK06_056 <400> 422 atgtttgtag caccacttcg acca 24 <210> 423 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK06_056 <400> 423 gggctgctgc ctggggatta at 22 <210> 424 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK06_063 <400> 424 aaaatgttag aggaaaaatt gcagttttat tca 33 <210> 425 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK06_063 <400> 425 atgttagagg aaaaattgca gtttattcg 30 <210> 426 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK06_063 <400> 426 gaataaaaaa tgccaccctc tctaagttaa 30 <210> 427 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK06_067 <400> 427 cacatgtttt tagttaagac gagaaagtc 29 <210> 428 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK06_067 <400> 428 cacatgtttt tagttaagac gagaaagtt 29 <210> 429 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK06_067 <400> 429 ctagagacga agtgagtggc tgttt 25 <210> 430 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK06_075 <400> 430 gaacctctcc aaaatcagac aagga 25 <210> 431 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK06_075 <400> 431 aacctctcca aaatcagaca aggg 24 <210> 432 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK06_075 <400> 432 tcttcacttt ctaaatcttc ctagcagttt 30 <210> 433 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK06_081 <400> 433 caataaaggt ttgtgccaat aattccg 27 <210> 434 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK06_081 <400> 434 cacaataaag gtttgtgcca atatattcca 29 <210> 435 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK06_081 <400> 435 tgtggatggt tgaagagagg accaa 25 <210> 436 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK06_098 <400> 436 atgtctcatt agctgcctgt gtagt 25 <210> 437 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK06_098 <400> 437 gtctcattag ctgcctgtgt agc 23 <210> 438 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK06_098 <400> 438 ccacagactc caaatgatgc tggaa 25 <210> 439 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK06_105 <400> 439 tctctcagat gattgttcct ttgtac 26 <210> 440 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK06_105 <400> 440 atattctctc agatgattgt tcctttgtat 30 <210> 441 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK06_105 <400> 441 cgatggtgtt tatgctaaaa cttgcacaa 29 <210> 442 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK06_113 <400> 442 ataataaaga cactatggtt acatgttgtg 30 <210> 443 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK06_113 <400> 443 ataataaaga cactatggtt acatgttgtc 30 <210> 444 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK06_113 <400> 444 ctatatcacc attaaatgtc taggaacttt 30 <210> 445 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK06_121 <400> 445 atgtatgata attttgcttg tgtaccttc 29 <210> 446 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK06_121 <400> 446 catgtatgat aattttgctt gtgtaccttt 30 <210> 447 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK06_121 <400> 447 caaactacca tgctcaacaa tttctccat 29 <210> 448 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK06_129 <400> 448 cattcatgaa taatatcatc tacgagtcg 29 <210> 449 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK06_129 <400> 449 cattcatgaa taatatcatc tacgagtca 29 <210> 450 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK06_129 <400> 450 cagtgatcaa cctgccaggt acaaa 25 <210> 451 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK06_136 <400> 451 cttggaactt ataactggaa tggaag 26 <210> 452 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK06_136 <400> 452 gtcttggaac ttataactgg aatggaaa 28 <210> 453 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK06_136 <400> 453 tctccccgct cgacgggtta aa 22 <210> 454 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK06_139 <400> 454 ccaatagatc attcatatat gcaaaggct 29 <210> 455 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK06_139 <400> 455 caatagatca ttcatatatg caaaggcc 28 <210> 456 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK06_139 <400> 456 ctgctgtatg ggtgaagtct ctctt 25 <210> 457 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK06_145 <400> 457 caccacactc ctctcaagtt cc 22 <210> 458 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK06_145 <400> 458 ccaccacact cctctcaagt tct 23 <210> 459 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK06_145 <400> 459 gagtgggtg agagggctac gat 23 <210> 460 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK06_152 <400> 460 tcttcttctt cttgctctgc cc 22 <210> 461 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK06_152 <400> 461 cttcttcttc ttcttgctct gcct 24 <210> 462 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK06_152 <400> 462 ggatccttcc cttctctttg atattgat 28 <210> 463 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK06_157 <400> 463 cggattgatc acgtggaatt cagat 25 <210> 464 <211> 19 <212> DNA <213> artificial sequence <220> <223> SK06_157 <400> 464 gatcacgtgg aattcagac 19 <210> 465 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK06_157 <400> 465 aagaggccca aatcaagttg aagttgtat 29 <210> 466 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK06_162 <400> 466 gcagtattac catgtgaggg tct 23 <210> 467 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK06_162 <400> 467 gcagtattac catgtgaggg tcg 23 <210> 468 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK06_162 <400> 468 ctccactaaa aagcctattg acaatggtt 29 <210> 469 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK06_170 <400> 469 aacgacagcg ggaatttgtc cc 22 <210> 470 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK06_170 <400> 470 gaacgacagc gggaatttgt cct 23 <210> 471 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK06_170 <400> 471 cttcggatac ttgggcattg aaggat 26 <210> 472 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK06_177 <400> 472 cattagtaga ccgtgatcac agaca 25 <210> 473 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK06_177 <400> 473 agtagaccgt gatcacagac c 21 <210> 474 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK06_177 <400> 474 tgcaaggttt catgacattt cagttggaa 29 <210> 475 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK06_183 <400> 475 acacttctaa ggtaatacta acaaactaat ata 33 <210> 476 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK06_183 <400> 476 cacttctaag gtaatactaa caaactaata tc 32 <210> 477 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK06_183 <400> 477 tgcgttgtta gttctgtcaa gcataagaa 29 <210> 478 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK06_191 <400> 478 aaattcaaga gctgaatcgt agaacca 27 <210> 479 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK06_191 <400> 479 aaattcaaga gctgaatcgt agaacct 27 <210> 480 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK06_191 <400> 480 cggaccttaa tttcatggag gtgtgta 27 <210> 481 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK06_190 <400> 481 gatataacta acaaagtatc ctttttgatc ta 32 <210> 482 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK06_190 <400> 482 gatataacta acaaagtatc ctttttgatc tc 32 <210> 483 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK06_190 <400> 483 ccattaatcc aaaccatatg ttactgtgtt 30 <210> 484 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK06_198 <400> 484 aagaaatatt ggaaaaagcc atggagtta 29 <210> 485 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK06_198 <400> 485 aagaaatatt ggaaaaagcc atggagttt 29 <210> 486 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK06_198 <400> 486 gaagtgagcg ttggaggtgt gtttt 25 <210> 487 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK06_205 <400> 487 agatgaggga cattgttgaa agttatattatt 29 <210> 488 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK06_205 <400> 488 gatgagggac attgttgaaa gttatatg 28 <210> 489 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK06_205 <400> 489 ggatttgaat cacacatgcta aaatcagcaa 30 <210> 490 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK06_212 <400> 490 gaaaacggtt cttttcttac cctgc 25 <210> 491 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK06_212 <400> 491 aagaaaacgg ttcttttctt accctgt 27 <210> 492 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK06_212 <400> 492 caaattagaa gagggaaaat tgggggtt 28 <210> 493 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK06_222 <400> 493 tatcagcaaa aagtgtaaag aaaaaaacag aa 32 <210> 494 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK06_222 <400> 494 cagcaaaaag tgtaaagaaa aaaacagag 29 <210> 495 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK06_222 <400> 495 gggtaggact ttggttggtt gtacaa 26 <210> 496 <211> 19 <212> DNA <213> artificial sequence <220> <223> SK06_235 <400> 496 gagcactggg cgcctatct 19 <210> 497 <211> 19 <212> DNA <213> artificial sequence <220> <223> SK06_235 <400> 497 gagcactggg cgcctatcc 19 <210> 498 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK06_235 <400> 498 agaagccctg tgcgcagtcc ta 22 <210> 499 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK06_240 <400> 499 accttattt tatatgtgtt tctattgttt tat 33 <210> 500 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK06_240 <400> 500 accttattt tatatgtgtt tctattgttt taa 33 <210> 501 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK06_240 <400> 501 cgcccccgat actctttttc ttttatttt 29 <210> 502 <211> 20 <212> DNA <213> artificial sequence <220> <223> SK06_249 <400> 502 cgcgacgccg cctgtattga 20 <210> 503 <211> 19 <212> DNA <213> artificial sequence <220> <223> SK06_249 <400> 503 gcgacgccgc ctgtattgg 19 <210> 504 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK06_249 <400> 504 atttatcat taccgtcggc aggcaattt 29 <210> 505 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK06_256 <400> 505 agtctcgaag agatgaagat tattgac 27 <210> 506 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK06_256 <400> 506 ataagtctcg aagagatgaa gattattgaa 30 <210> 507 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK06_256 <400> 507 ggccatctct acgtatagac atgtatatt 29 <210> 508 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK07_006 <400> 508 gagatgtgca gttgttgcag cag 23 <210> 509 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK07_006 <400> 509 gagatgtgca gttgttgcag cat 23 <210> 510 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK07_006 <400> 510 gaacctgggaa acctttgttt gtatccat 28 <210> 511 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK07_022 <400> 511 atacaattat tcttactttc tttcaagttt tt 32 <210> 512 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK07_022 <400> 512 atacaattat tcttactttc tttcaagttt tc 32 <210> 513 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK07_022 <400> 513 cagaggttcg cactccaata cttaaatta 29 <210> 514 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK07_043 <400> 514 cctccttgtt ttctttcctt ttttc 25 <210> 515 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK07_043 <400> 515 cctcctcctt gttttctttc cttttttt 28 <210> 516 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK07_043 <400> 516 cggattccaa acgaaaagga caagaatta 29 <210> 517 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK07_047 <400> 517 gaacttact tttgtttcta tagataagca g 31 <210> 518 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK07_047 <400> 518 ggaactttac ttttgtttct atagataagc aa 32 <210> 519 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK07_047 <400> 519 aggaacgaaa agggaaagaa cacatgtt 28 <210> 520 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK07_055 <400> 520 tctttaacgt aaccacttgc tcg 23 <210> 521 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK07_055 <400> 521 cttctttaac gtaaccactt gctca 25 <210> 522 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK07_055 <400> 522 gaatgacact cgtatccaac agtctattt 29 <210> 523 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK07_064 <400> 523 ttacgtttgt ccaacaacta gccc 24 <210> 524 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK07_064 <400> 524 ttacgtttgt ccaacaacta gcca 24 <210> 525 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK07_064 <400> 525 gggcattttg ctcatttcac cacaaaata 29 <210> 526 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK07_073 <400> 526 ttggctggtt aacctgttca tcaaa 25 <210> 527 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK07_073 <400> 527 tggctggtta acctgttcat caat 24 <210> 528 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK07_073 <400> 528 cagttcacat ggccaaacct ggaaa 25 <210> 529 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK07_079 <400> 529 ggaatatcat gttgcatcaa tatcaccat 29 <210> 530 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK07_079 <400> 530 ggaatatcat gttgcatcaa tatcaccaa 29 <210> 531 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK07_079 <400> 531 gtagtttgat caatttcttc tgcagcagat 30 <210> 532 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK07_083 <400> 532 agtattataa ggaccatatc aattttctgg 30 <210> 533 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK07_083 <400> 533 agtattataa ggaccatatc aattttctgc 30 <210> 534 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK07_083 <400> 534 gaagacatga agtcctatca atatgctatt 30 <210> 535 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK07_094 <400> 535 cgagaaactt ctacacttgg attcg 25 <210> 536 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK07_094 <400> 536 aacgagaaac ttctacactt ggattca 27 <210> 537 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK07_094 <400> 537 cactaataat tctctaccta acttcctcaa 30 <210> 538 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK07_100 <400> 538 aaacttcgag ggtgctaaat ttaatttttc 30 <210> 539 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK07_100 <400> 539 caaacttcga ggggtgctaaa tttaattttt t 31 <210> 540 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK07_100 <400> 540 ctcgtttcct ccgagatttg ttgtaatta 29 <210> 541 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK07_106 <400> 541 gtatatgtta taattgtaat gctgtggag 29 <210> 542 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK07_106 <400> 542 cttgtatatg ttataattgt aatgctgtgg aa 32 <210> 543 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK07_106 <400> 543 ccatgtaatc cacaaatatg agataaggta 30 <210> 544 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK07_114 <400> 544 aatctatgtt tgttttttga ttggtcacg 29 <210> 545 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK07_114 <400> 545 ataatctatg tttgtttttt gattggtcac a 31 <210> 546 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK07_114 <400> 546 caaagtcgga cttgtgcttc cgaat 25 <210> 547 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK07_126 <400> 547 ataagtcccc agatggtggt aaatg 25 <210> 548 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK07_126 <400> 548 cataagtccc cagatggtgg taaata 26 <210> 549 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK07_126 <400> 549 cgtctggtcg agatgggcaa atata 25 <210> 550 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK07_133 <400> 550 gtggattctg attattggtt ttacgaattt a 31 <210> 551 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK07_133 <400> 551 ggattctgat tattggtttt acgaatttg 29 <210> 552 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK07_133 <400> 552 ggggccatgt ataaaggagt tccta 25 <210> 553 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK07_130 <400> 553 tctttctgga ttatttgttg tccacc 26 <210> 554 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK07_130 <400> 554 ctttctggat tatttgttgt ccacg 25 <210> 555 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK07_130 <400> 555 ggagaaatgg cagcattagt tcatacat 28 <210> 556 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK07_145 <400> 556 gacattttgg caacccttgc agc 23 <210> 557 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK07_145 <400> 557 atgacatttt ggcaaccctt gcaga 25 <210> 558 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK07_145 <400> 558 aaatacctca tggagtggtt cagacttat 29 <210> 559 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK07_151 <400> 559 ctcttttcac aatttaggggg ttcact 26 <210> 560 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK07_151 <400> 560 ctcttttcac aatttaggggg ttcacg 26 <210> 561 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK07_151 <400> 561 tctgcttacc gaagtcttct tactgtaaa 29 <210> 562 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK07_164 <400> 562 ctcctcaatc tacaaactga tatgca 26 <210> 563 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK07_164 <400> 563 ctcctcaatc tacaaactga tatgct 26 <210> 564 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK07_164 <400> 564 atatatctca tgagcctaaa aaactgatat 30 <210> 565 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK07_166 <400> 565 gcgcttcacc atttctccct t 21 <210> 566 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK07_166 <400> 566 ctgcgcttca ccatttctcc cta 23 <210> 567 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK07_166 <400> 567 cagagcatac cagtactgta attgacaaa 29 <210> 568 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK08_001 <400> 568 agattatgga tactcatagt agcttact 28 <210> 569 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK08_001 <400> 569 agattatgga tactcatagt agcttaca 28 <210> 570 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK08_001 <400> 570 acccttccct ctgcggcaca a 21 <210> 571 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK08_008 <400> 571 catggccgcc ctcttcttca tt 22 <210> 572 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK08_008 <400> 572 atggccgccc tcttcttcat g 21 <210> 573 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK08_008 <400> 573 catgtgcgga ggtagaaaac agcat 25 <210> 574 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK08_012 <400> 574 agaggtctct aaccggttat tgtg 24 <210> 575 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK08_012 <400> 575 gtagaggtct ctaaccggtt attgta 26 <210> 576 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK08_012 <400> 576 tttccatgaa atcaatgctt ttcctaggaa 30 <210> 577 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK08_020 <400> 577 gaattagtga agcagaaaga tttggca 27 <210> 578 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK08_020 <400> 578 aattagtgaa gcagaaagat ttggcg 26 <210> 579 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK08_020 <400> 579 acccctcctg caattccagc aaaat 25 <210> 580 <211> 16 <212> DNA <213> artificial sequence <220> <223> SK08_032 <400> 580 ggaagagcag gcaccg 16 <210> 581 <211> 20 <212> DNA <213> artificial sequence <220> <223> SK08_032 <400> 581 agctggaaga gcaggcacca 20 <210> 582 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK08_032 <400> 582 gctcccccta ttcaatttgg ggaaa 25 <210> 583 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK08_039 <400> 583 cctccgaccg gataaactcc a 21 <210> 584 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK08_039 <400> 584 cctccgaccg gataaactcc g 21 <210> 585 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK08_039 <400> 585 cgagtttgaa aaggatcttg tttgcagta 29 <210> 586 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK08_046 <400> 586 aaggtttcat tattaaatat tgccataaaa c 31 <210> 587 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK08_046 <400> 587 ctaaggtttc attattaaat attgccataa aat 33 <210> 588 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK08_046 <400> 588 taggcagcaa gaaaatgaga cactgaaaa 29 <210> 589 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK08_052 <400> 589 atacaagtaa tatatgcact acacacttag 30 <210> 590 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK08_052 <400> 590 atacaagtaa tatatgcact acacacttac 30 <210> 591 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK08_052 <400> 591 caatatcacc actcagacaa gaaatcgtt 29 <210> 592 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK08_066 <400> 592 gatttggggc cttcaatcct gc 22 <210> 593 <211> 18 <212> DNA <213> artificial sequence <220> <223> SK08_066 <400> 593 tggggccttc aatcctgt 18 <210> 594 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK08_066 <400> 594 gaagcacgcc gatggaagag gtt 23 <210> 595 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK08_072 <400> 595 aaaaaagcaa aaaaaggaga aacaaatctt gaa 33 <210> 596 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK08_072 <400> 596 aaaaaagcaa aaaaaggaga aacaaatctt gat 33 <210> 597 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK08_072 <400> 597 aacttccact cagaatcata tgacttgttt 30 <210> 598 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK08_125 <400> 598 acttgaaaat cgtgccattg tgct 24 <210> 599 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK08_125 <400> 599 acttgaaaat cgtgccattg tgcc 24 <210> 600 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK08_125 <400> 600 tacttgatgg tccttgaacg atggaattt 29 <210> 601 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK08_120 <400> 601 aattacttta aatattgtcg caatcaataa tca 33 <210> 602 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK08_120 <400> 602 aattacttta aatattgtcg caatcaataa tcc 33 <210> 603 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK08_120 <400> 603 catagtatga cgattttgga ttccttacat 30 <210> 604 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK08_137 <400> 604 atatagattg acagagcgtg ccca 24 <210> 605 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK08_137 <400> 605 atagatgac agagcgtgcc cg 22 <210> 606 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK08_137 <400> 606 gttagtggggc ttggtcattt catttacat 29 <210> 607 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK08_142 <400> 607 caaacagaga aacagaatta ttagaacttg t 31 <210> 608 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK08_142 <400> 608 aaacagagaa acagaattat tagaacttgc 30 <210> 609 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK08_142 <400> 609 cagtggaaat cttagtggaa accttagta 29 <210> 610 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK08_148 <400> 610 ggggatgttt tgtatccgcg ac 22 <210> 611 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK08_148 <400> 611 cggggatgtt ttgtatccgc gat 23 <210> 612 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK08_148 <400> 612 ctgatccgga gccgcttaat gatta 25 <210> 613 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK08_156 <400> 613 tatagtgtga gatccttagt gtatcc 26 <210> 614 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK08_156 <400> 614 ctttatagtgt gagatcctta gtgtatca 28 <210> 615 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK08_156 <400> 615 cgacctcaac catccagctc gaa 23 <210> 616 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK08_164 <400> 616 catttattat gccaccttta ggaacagaa 29 <210> 617 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK08_164 <400> 617 tattatgcca cctttaggaa cagag 25 <210> 618 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK08_164 <400> 618 gaagcagcaa ttttgcagac ctgcta 26 <210> 619 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK08_172 <400> 619 gtcgaggaac gacaacaaca cga 23 <210> 620 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK08_172 <400> 620 cgaggaacga caacaacacg g 21 <210> 621 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK08_172 <400> 621 cgtcagagtt attagttaact cttcccaat 29 <210> 622 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK08_179 <400> 622 aaatcattac cttgtttctc gtgcttg 27 <210> 623 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK08_179 <400> 623 aaatcattac cttgtttctc gtgcttc 27 <210> 624 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK08_179 <400> 624 ggttttacct cccatgcagt cctt 24 <210> 625 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK08_187 <400> 625 aactacgatg aatttcaata tgagaacact 30 <210> 626 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK08_187 <400> 626 ctacgatgaa tttcaatatg agaacacc 28 <210> 627 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK08_187 <400> 627 cgatttcgtg attcttttac agtaaactat 30 <210> 628 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK08_197 <400> 628 ggatttaaat ggacatttag gtcaattcat 30 <210> 629 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK08_197 <400> 629 taaatggaca tttaggtcaa ttcaa 25 <210> 630 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK08_197 <400> 630 cgatcgttca acaataaatc tatcccgtt 29 <210> 631 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK08_203 <400> 631 gtgaaaatcc attatacaga tcatcaaac 29 <210> 632 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK08_203 <400> 632 ctgtgaaaat ccattataca gatcatcaaa a 31 <210> 633 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK08_203 <400> 633 gctgttaaag atgtatcgaa ttcgcagaa 29 <210> 634 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK08_209 <400> 634 catcatcttc atcatcttct acgtcatat 29 <210> 635 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK08_209 <400> 635 catcttcatc atcttctacg tcatac 26 <210> 636 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK08_209 <400> 636 gcggtcttct aaaagcaagt cgataatta 29 <210> 637 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK08_216 <400> 637 atttagattt gggttcagga gctaagt 27 <210> 638 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK08_216 <400> 638 tagatttggg ttcaggagct aaga 24 <210> 639 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK08_216 <400> 639 tttcaacttt ctatcttgtt gcgctggat 29 <210> 640 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK08_219 <400> 640 aaatgaacac ttatgatgat taaacttttc tta 33 <210> 641 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK08_219 <400> 641 aaatgaacac ttatgatgat taaacttttc ttt 33 <210> 642 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK08_219 <400> 642 tttggtccag tttctcgcac ccttt 25 <210> 643 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK08_227 <400> 643 gataaccatg tgatctttta acgtcagt 28 <210> 644 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK08_227 <400> 644 ataaccatgt gatcttttaa cgtcagc 27 <210> 645 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK08_227 <400> 645 gatccatgga cctattcata atacaagaat 30 <210> 646 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK08_233 <400> 646 ataggtcaaa agagcggcgg t 21 <210> 647 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK08_233 <400> 647 ataggtcaaa agagcggcgg c 21 <210> 648 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK08_233 <400> 648 atgccgctct ccgcctcctt ta 22 <210> 649 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK08_241 <400> 649 gcctgacagt tccattccag aatt 24 <210> 650 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK08_241 <400> 650 gcctgacagt tccattccag aata 24 <210> 651 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK08_241 <400> 651 ccattggtgt tacaagattc tcttttcatt 30 <210> 652 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK08_254 <400> 652 tctgccatga aagcagcgct g 21 <210> 653 <211> 20 <212> DNA <213> artificial sequence <220> <223> SK08_254 <400> 653 ctgccatgaa agcagcgctc 20 <210> 654 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK08_254 <400> 654 ggagaaccaa agggtgtcat cctat 25 <210> 655 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK08_260 <400> 655 tttcatacag atcgtagcta gttg 24 <210> 656 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK08_260 <400> 656 ccttttcata cagatcgtag ctagttt 27 <210> 657 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK08_260 <400> 657 cggctgcaga acatgagaaa ctcat 25 <210> 658 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK09_004 <400> 658 atactactcc ttccagtttt gggtc 25 <210> 659 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK09_004 <400> 659 gatactactc cttccagttt tgggtt 26 <210> 660 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK09_004 <400> 660 ctcatcgtta cttgcagagt tttgatctt 29 <210> 661 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK09_028 <400> 661 acattcagac gaccataagt attagac 27 <210> 662 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK09_028 <400> 662 cacattcaga cgaccataag tattagat 28 <210> 663 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK09_028 <400> 663 cctggattcg ttctctttcc gtaattatt 29 <210> 664 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK09_035 <400> 664 tttacaattt cttagattcc atggtattg 29 <210> 665 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK09_035 <400> 665 aacttttaca atttcttaga ttccatggta ttt 33 <210> 666 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK09_035 <400> 666 gagaacttat tgacactaga atctagcata 30 <210> 667 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK09_042 <400> 667 gtagaagtca aagatggata atatggttc 29 <210> 668 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK09_042 <400> 668 gtagaagtca aagatggata atatggttt 29 <210> 669 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK09_042 <400> 669 tgagtccatc aaaatcacac atcacctat 29 <210> 670 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK09_052 <400> 670 atttctctgt gactccgttt cacacat 26 <210> 671 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK09_052 <400> 671 tctctgtgac tccgtttcaa caa 23 <210> 672 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK09_052 <400> 672 ctgcggaaca tttggtggtt caaaatatt 29 <210> 673 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK09_056 <400> 673 aaaagcaact tcccgaattc gagaaaa 27 <210> 674 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK09_056 <400> 674 aagcaacttc ccgaattcga gaaag 25 <210> 675 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK09_056 <400> 675 gtgtttgcag tagatagttc aaattcagtt 30 <210> 676 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK09_062 <400> 676 cttgttgctt agccaatggc aact 24 <210> 677 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK09_062 <400> 677 gttgcttagc caatggcaac c 21 <210> 678 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK09_062 <400> 678 gtccgagtct ggaagacaga actat 25 <210> 679 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK09_068 <400> 679 ggcataagta atatagcaca gactgtaa 28 <210> 680 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK09_068 <400> 680 gcataagtaa tatagcacag actgtag 27 <210> 681 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK09_068 <400> 681 gattcttgta gcagactgtc caatacttt 29 <210> 682 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK09_075 <400> 682 atttaaaaat actcacagta tccatctgg 29 <210> 683 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK09_075 <400> 683 ttatttaaaa atactcacag tatccatctg a 31 <210> 684 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK09_075 <400> 684 cggagtagag acaaaaatgc atggctt 27 <210> 685 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK09_093 <400> 685 caaaaatttt atatataact gcaccaagtc ata 33 <210> 686 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK09_093 <400> 686 aaaaatttta tatataactg caccaagtca tg 32 <210> 687 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK09_093 <400> 687 cattatgatt aaaccggaaa ggtaaaggaa 30 <210> 688 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK09_123 <400> 688 catgaacttc atgacgcaac aaacga 26 <210> 689 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK09_123 <400> 689 atgaacttca tgacgcaaca aacgg 25 <210> 690 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK09_123 <400> 690 cggcggttgt agagttcccc at 22 <210> 691 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK09_132 <400> 691 cgttaggttt ggacgaaaaa tactgaa 27 <210> 692 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK09_132 <400> 692 gttaggtttg gacgaaaaat actgag 26 <210> 693 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK09_132 <400> 693 attcctcaag aatgttaaat aagaggtaaa 30 <210> 694 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK09_140 <400> 694 aaggataaag gaaagatggc ggc 23 <210> 695 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK09_140 <400> 695 caaggataaa ggaaagatgg cgga 24 <210> 696 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK09_140 <400> 696 gttctgtaca actcatcatc tccttgtat 29 <210> 697 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK09_154 <400> 697 ccgttaacca aagacattac tgctg 25 <210> 698 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK09_154 <400> 698 ccgttaacca aagacattac tgctc 25 <210> 699 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK09_154 <400> 699 agaagaagta atgggaagag tccagttt 28 <210> 700 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK09_162 <400> 700 caagggttgt acttcttctc agat 24 <210> 701 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK09_162 <400> 701 caagggttgt acttcttctc agag 24 <210> 702 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK09_162 <400> 702 ctgtgatggc tcaaatccat gatcaaaat 29 <210> 703 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK09_160 <400> 703 cactgtatgg tgaattgaat ctgctt 26 <210> 704 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK09_160 <400> 704 cactgtatgg tgaattgaat ctgcta 26 <210> 705 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK09_160 <400> 705 tctatcctac tccacaattg atcctcttt 29 <210> 706 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK09_169 <400> 706 tataagccta actgtctttg ctttc 25 <210> 707 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK09_169 <400> 707 gcttataagc ctaactgtct ttgctttt 28 <210> 708 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK09_169 <400> 708 gcctcgttga attcagaagc gcaaa 25 <210> 709 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK09_173 <400> 709 gggcggagaa aagacatgag c 21 <210> 710 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK09_173 <400> 710 ggggcggaga aaagacatga ga 22 <210> 711 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK09_173 <400> 711 ctcggctcct ggctctccaa at 22 <210> 712 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK09_177 <400> 712 ttcttcagaa gctctgtttt tattacttc 29 <210> 713 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK09_177 <400> 713 ttttcttcag aagctctgtt tttattactt t 31 <210> 714 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK09_177 <400> 714 agaatagcaa cattaggtcg agcttcaaa 29 <210> 715 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK09_181 <400> 715 accccagtat ttaaggagat gg 22 <210> 716 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK09_181 <400> 716 gctaccccag tatttaagga gatga 25 <210> 717 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK09_181 <400> 717 agtcttcttg cataggcaca aactcattt 29 <210> 718 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK09_186 <400> 718 caaaatccct tcaattatca ttttacgag 29 <210> 719 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK09_186 <400> 719 caaaatccct tcaattatca ttttacgac 29 <210> 720 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK09_186 <400> 720 gtctcgatct atcctgatat tggacaaa 28 <210> 721 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK09_189 <400> 721 aaccagatgc ggaccgaatc ag 22 <210> 722 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK09_189 <400> 722 aaaccagatg cggaccgaat caa 23 <210> 723 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK09_189 <400> 723 aaatcagcat attcctccca taaagacaaa 30 <210> 724 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK09_197 <400> 724 ctcatgcctt tttctgtcaa ccca 24 <210> 725 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK09_197 <400> 725 ctcatgcctt tttctgtcaa ccct 24 <210> 726 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK09_197 <400> 726 cctggtttaa catcaagaat cagaaggaa 29 <210> 727 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK09_208 <400> 727 gttcaaccgt atctcttctt ctcaat 26 <210> 728 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK09_208 <400> 728 gttcaaccgt atctcttctt ctcaac 26 <210> 729 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK09_208 <400> 729 gacgaatcga aaaataagca tacacgcat 29 <210> 730 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK09_213 <400> 730 gtagttgcct ttttgacgcc gtaaa 25 <210> 731 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK09_213 <400> 731 agttgccttt ttgacgccgt aac 23 <210> 732 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK09_213 <400> 732 ctcctggctt tttatgcttg tatggataa 29 <210> 733 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK09_224 <400> 733 atatcggaaa gtggaaacaa gcaaaac 27 <210> 734 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK09_224 <400> 734 gatatcggaa agtggaaaca agcaaaaa 28 <210> 735 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK09_224 <400> 735 gcccacatct gggaactcat tataaattt 29 <210> 736 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK09_228 <400> 736 caaaatcaag cctgtcatca tttctaag 28 <210> 737 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK09_228 <400> 737 atcaaaatca agcctgtcat catttctaaa 30 <210> 738 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK09_228 <400> 738 gtctgtaact tgatacgata aagtggctt 29 <210> 739 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK10_005 <400> 739 cgtcgtcgtc gatgataaat ggt 23 <210> 740 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK10_005 <400> 740 cgtcgtcgtc gatgataaat ggc 23 <210> 741 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK10_005 <400> 741 ggatgagcat gaacgcggca ttaat 25 <210> 742 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK10_011 <400> 742 gtaggatcaa atcagcatcg tttgaa 26 <210> 743 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK10_011 <400> 743 gtaggatcaa atcagcatcg tttgat 26 <210> 744 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK10_011 <400> 744 gagaggtagg ggcaaaatgg gaaat 25 <210> 745 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK10_014 <400> 745 aaattgtata tgagaatgct taatacaata tgc 33 <210> 746 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK10_014 <400> 746 aaattgtata tgagaatgct taatacaata tgg 33 <210> 747 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK10_014 <400> 747 ctttggttgt ttgcgtgact atgaacatt 29 <210> 748 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK10_019 <400> 748 gaaatctcct ccgatgatga ggaa 24 <210> 749 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK10_019 <400> 749 aaatctcctc cgatgatgag gac 23 <210> 750 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK10_019 <400> 750 gtaggaacgg ctagtcctcg gaa 23 <210> 751 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK10_028 <400> 751 aaatctgagc tcgagctcga cg 22 <210> 752 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK10_028 <400> 752 aaaaatctga gctcgagctc gaca 24 <210> 753 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK10_028 <400> 753 ctcgagttca agctcgagcc tttat 25 <210> 754 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK10_032 <400> 754 ataatgcacc tgatccgcag cc 22 <210> 755 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK10_032 <400> 755 ataatgcacc tgatccgcag ct 22 <210> 756 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK10_032 <400> 756 tagaggatgt gaatgatgag gtggtttt 28 <210> 757 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK10_038 <400> 757 cactaacaag aacaagccgg gg 22 <210> 758 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK10_038 <400> 758 aacactaaca agaacaagcc gggt 24 <210> 759 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK10_038 <400> 759 cgggtgattt tggctctccc aaaaa 25 <210> 760 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK10_046 <400> 760 aacacaacaa ctatcagaaa atgttccat 29 <210> 761 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK10_046 <400> 761 cacaacaact atcagaaaat gttccac 27 <210> 762 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK10_046 <400> 762 ctcgtttcta attgtcgtag ctattggtt 29 <210> 763 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK10_053 <400> 763 gaaagtgctc tcatctttac atacaaat 28 <210> 764 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK10_053 <400> 764 gaaagtgctc tcatctttac atacaaaa 28 <210> 765 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK10_053 <400> 765 ccctctctgc cttcatttct tcagat 26 <210> 766 <211> 19 <212> DNA <213> artificial sequence <220> <223> SK10_061 <400> 766 ttccccaaaa cgagcttcc 19 <210> 767 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK10_061 <400> 767 gctttcccca aaacgagctt cg 22 <210> 768 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK10_061 <400> 768 cctcacacga attatggctc gagtt 25 <210> 769 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK10_076 <400> 769 tgctcttgta atatgggtca tcag 24 <210> 770 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK10_076 <400> 770 cttgctcttg taatatgggt catcaa 26 <210> 771 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK10_076 <400> 771 ccctgtgtgc catatgaact tccaa 25 <210> 772 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK10_086 <400> 772 tcaggatgtg gggtttcggt tta 23 <210> 773 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK10_086 <400> 773 caggatgtgg ggtttcggtt tc 22 <210> 774 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK10_086 <400> 774 caataatttg gtaagtgggt ctgttttgat 30 <210> 775 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK10_092 <400> 775 aatcaagcct aaatggccac tcg 23 <210> 776 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK10_092 <400> 776 caatcaagcc taaatggcca ctca 24 <210> 777 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK10_092 <400> 777 ggtccatgga aaaagaagtt tgcattcat 29 <210> 778 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK10_097 <400> 778 atggaaatgg aattctactg cac 23 <210> 779 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK10_097 <400> 779 gctatggaaa tggaattcta ctgcat 26 <210> 780 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK10_097 <400> 780 ctgcaatagc agaatcatgg gaattctaa 29 <210> 781 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK10_113 <400> 781 acctaaaatc cacttctgaa gtattc 26 <210> 782 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK10_113 <400> 782 aactacctaa aatccacttc tgaagtattt 30 <210> 783 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK10_113 <400> 783 cgtctcgaat tcattttatt tgggacagat 30 <210> 784 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK10_123 <400> 784 tagcaataat tcagatacgt tcctcg 26 <210> 785 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK10_123 <400> 785 gttagcaata attcagatac gttcctca 28 <210> 786 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK10_123 <400> 786 tcaattctgc agcctgagta atggaatt 28 <210> 787 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK10_128 <400> 787 ttaagtaatt gctgggtttg ttcgga 26 <210> 788 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK10_128 <400> 788 aagtaattgc tgggtttgtt cggg 24 <210> 789 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK10_128 <400> 789 gaagcaaaca ccagaaacat gctaaacaa 29 <210> 790 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK10_131 <400> 790 acaaactagt ttaattaata atatacccat tgc 33 <210> 791 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK10_131 <400> 791 acaaactagt ttaattaata atatacccat tgt 33 <210> 792 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK10_131 <400> 792 ctcttgatga tatgaggcaa tggatctaa 29 <210> 793 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK10_135 <400> 793 ttttaagggg aacttttcaa tttttttttt cc 32 <210> 794 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK10_135 <400> 794 tttaagggga acttttcaat tttttttttc g 31 <210> 795 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK10_135 <400> 795 tactctcagg gactcttata actaacctt 29 <210> 796 <211> 20 <212> DNA <213> artificial sequence <220> <223> SK10_146 <400> 796 attcttgctt tgcaccgccg 20 <210> 797 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK10_146 <400> 797 gctattcttg ctttgcaccg cct 23 <210> 798 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK10_146 <400> 798 acaccaggtg gctgccgcta t 21 <210> 799 <211> 19 <212> DNA <213> artificial sequence <220> <223> SK10_153 <400> 799 ggccaacgtg caatccact 19 <210> 800 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK10_153 <400> 800 ctggccaacg tgcaatccac a 21 <210> 801 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK10_153 <400> 801 ccttcagcgg gacctccagt tt 22 <210> 802 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK10_161 <400> 802 accaatacaa acttgcatgt cccg 24 <210> 803 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK10_161 <400> 803 accaatacaa acttgcatgt cccc 24 <210> 804 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK10_161 <400> 804 ggtggagctt tttggccaag ttgaa 25 <210> 805 <211> 19 <212> DNA <213> artificial sequence <220> <223> SK10_167 <400> 805 ggccggagat tgcagcagc 19 <210> 806 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK10_167 <400> 806 atggccggag attgcagcag t 21 <210> 807 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK10_167 <400> 807 cgtttccatc tccatccaca gtcta 25 <210> 808 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK10_174 <400> 808 ccaagtccaa acgtcaccag c 21 <210> 809 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK10_174 <400> 809 tccaagtcca aacgtcacca gt 22 <210> 810 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK10_174 <400> 810 catatgctcc tgcagattgg ccaaa 25 <210> 811 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK10_182 <400> 811 acaaacacca tcccaatatc tagcaat 27 <210> 812 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK10_182 <400> 812 acaaacacca tcccaatatc tagcaaa 27 <210> 813 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK10_182 <400> 813 gtgctgattc ttctcaaatt cctcgtaaa 29 <210> 814 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK11_001 <400> 814 gatggactga tatcgcaaat caacca 26 <210> 815 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK11_001 <400> 815 atggactgat atcgcaaatc aaccg 25 <210> 816 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK11_001 <400> 816 gcaatttcag ggactggtac gactt 25 <210> 817 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK11_007 <400> 817 ttgaacaaga ctgacaaggt tat 23 <210> 818 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK11_007 <400> 818 gctttgaaca agactgacaa ggttac 26 <210> 819 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK11_007 <400> 819 ggcttttcat ctgcattctg ggatgat 27 <210> 820 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK11_015 <400> 820 ttcagtacaa gacgctgctg aagt 24 <210> 821 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK11_015 <400> 821 cagtacaaga cgctgctgaa gc 22 <210> 822 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK11_015 <400> 822 cagagtagcc gctcggacat gtt 23 <210> 823 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK11_022 <400> 823 tccccttcat tttccccctc c 21 <210> 824 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK11_022 <400> 824 cttccccttc attttccccc tct 23 <210> 825 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK11_022 <400> 825 ccactgttgt tgctcatcgt ctgat 25 <210> 826 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK11_027 <400> 826 ataatgttgg cttccatcgg caact 25 <210> 827 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK11_027 <400> 827 atgttggctt ccatcggcaa cc 22 <210> 828 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK11_027 <400> 828 ttaattacct gatgtgggat ccacgtatt 29 <210> 829 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK11_035 <400> 829 tagactgtct tcacagtcgc tagt 24 <210> 830 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK11_035 <400> 830 gactgtcttc acagtcgcta gc 22 <210> 831 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK11_035 <400> 831 cagccacttt cccctctcct gaa 23 <210> 832 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK11_042 <400> 832 tgatttactt cgaacctcct tcg 23 <210> 833 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK11_042 <400> 833 gcttgattta cttcgaacct ccttca 26 <210> 834 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK11_042 <400> 834 acgttgaagt ttcgcgtgaa cagattaaa 29 <210> 835 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK11_050 <400> 835 ctggaaaacc cctaatttgg ccta 24 <210> 836 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK11_050 <400> 836 ggaaaacccc taatttggcc tg 22 <210> 837 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK11_050 <400> 837 ggtatgagag gtgtatgggt acagtt 26 <210> 838 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK11_059 <400> 838 ctcactaaaa gcaaacataa atcaaatcg 29 <210> 839 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK11_059 <400> 839 ctcactaaaa gcaaacataa atcaaatcc 29 <210> 840 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK11_059 <400> 840 cactcggcca caaaatctcc ttagat 26 <210> 841 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK11_066 <400> 841 gtgagaaaga gtgaggttct tgc 23 <210> 842 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK11_066 <400> 842 aggtgagaaa gagtgaggtt cttgt 25 <210> 843 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK11_066 <400> 843 acatcccacc aattaagcaa tggattctt 29 <210> 844 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK11_073 <400> 844 agcttataag cattttagca tctggac 27 <210> 845 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK11_073 <400> 845 agcttataag cattttagca tctggag 27 <210> 846 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK11_073 <400> 846 gttcaccaat attctggggag taattgcaa 29 <210> 847 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK11_084 <400> 847 gaaactccca ccaaagacgg tg 22 <210> 848 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK11_084 <400> 848 gaaactccca ccaaagacgg ta 22 <210> 849 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK11_084 <400> 849 gccggcgtgt cactagaatc cat 23 <210> 850 <211> 20 <212> DNA <213> artificial sequence <220> <223> SK11_092 <400> 850 cttgatccga caagtctgcc 20 <210> 851 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK11_092 <400> 851 ctcttgatcc gacaagtctg ct 22 <210> 852 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK11_092 <400> 852 gcattctttg ttggtttgga caaaaccat 29 <210> 853 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK11_102 <400> 853 gtggtacctg tacggtcacc ta 22 <210> 854 <211> 20 <212> DNA <213> artificial sequence <220> <223> SK11_102 <400> 854 ggtacctgta cggtcacctc 20 <210> 855 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK11_102 <400> 855 ggcaaaaatg agctggccca tcaaa 25 <210> 856 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK11_109 <400> 856 gatcgatctt gttaccactt tataacg 27 <210> 857 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK11_109 <400> 857 ggatcgatct tgttaccact ttataaca 28 <210> 858 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK11_109 <400> 858 accgtccatc taaatcacaa cgtattgta 29 <210> 859 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK11_117 <400> 859 agccaaacga atcttaaata aatgaattgc 30 <210> 860 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK11_117 <400> 860 gagccaaacg aatcttaaat aaatgaattg t 31 <210> 861 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK11_117 <400> 861 ccattacttt gtagttccgt gtggaata 28 <210> 862 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK11_125 <400> 862 ttaaatgaaa tgttcattcc atacgccat 29 <210> 863 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK11_125 <400> 863 aaatgaaatg ttcattccat acgccac 27 <210> 864 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK11_125 <400> 864 agtggtgttc aaaaccttgt aagagatttt 30 <210> 865 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK11_130 <400> 865 ccatctttga gctaccaact taagtg 26 <210> 866 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK11_130 <400> 866 ccatctttga gctaccaact taagta 26 <210> 867 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK11_130 <400> 867 cagcaatgat gatgatgatg agatgagaa 29 <210> 868 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK11_137 <400> 868 gaccttatta gtcaaagaac atcctca 27 <210> 869 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK11_137 <400> 869 accttattag tcaaagaaca tcctcg 26 <210> 870 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK11_137 <400> 870 ctacaacttc tttttcaggt gctgacata 29 <210> 871 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK12_007 <400> 871 caatttgcaa gttcctatct tcattttttt g 31 <210> 872 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK12_007 <400> 872 ccaatttgca agttcctatc ttcatttttt tt 32 <210> 873 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK12_007 <400> 873 ccctctataa tattgtaaat gggtaaatta 30 <210> 874 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK12_019 <400> 874 gcaacgtagg cttgttggag ga 22 <210> 875 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK12_019 <400> 875 caacgtaggc ttgttggagg c 21 <210> 876 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK12_019 <400> 876 catgtgagca agaatgctac aaaccaaa 28 <210> 877 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK12_022 <400> 877 ggttgtttac ttattgagtc atgcaac 27 <210> 878 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK12_022 <400> 878 cggttgttta cttattgagt catgcaat 28 <210> 879 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK12_022 <400> 879 gtatatagaa ttcaatgtct acacctgaaa 30 <210> 880 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK12_033 <400> 880 gagtataaat tgtatacaac accccct 27 <210> 881 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK12_033 <400> 881 agtataaatt gtatacaaca cccccc 26 <210> 882 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK12_033 <400> 882 gggatcaagt cataacgtag ttcaaacaa 29 <210> 883 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK12_042 <400> 883 cgtccataga tccagttctt ctg 23 <210> 884 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK12_042 <400> 884 cgtccataga tccagttctt ctc 23 <210> 885 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK12_042 <400> 885 acatccaaat gaaaatcaag gtggagatat 30 <210> 886 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK12_052 <400> 886 acggtactac acaaaccttt ggtc 24 <210> 887 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK12_052 <400> 887 acggtactac acaaaccttt ggtg 24 <210> 888 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK12_052 <400> 888 gcaacaacta cttttgggtt tgtcaacaa 29 <210> 889 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK12_066 <400> 889 ttatccatca tacactatgt ataaagtgc 29 <210> 890 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK12_066 <400> 890 cttttatcca tcatacacta tgtataaagt ga 32 <210> 891 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK12_066 <400> 891 aggggacatt ttcttgtggt ttatagttta 30 <210> 892 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK12_073 <400> 892 aagaaaaaaa gaaagaagaa agcaagcaac 30 <210> 893 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK12_073 <400> 893 aaaaagaaaa aaagaaagaa gaaagcaagc aat 33 <210> 894 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK12_073 <400> 894 tgttatgcta ataatgccca aagttgattt 30 <210> 895 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK12_080 <400> 895 gtaaacattc tcctgataaa ctaatgtgtt a 31 <210> 896 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK12_080 <400> 896 aaacattctc ctgataaact aatgtgttg 29 <210> 897 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK12_080 <400> 897 ctacaacagg gaagattctg aattggtat 29 <210> 898 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK12_089 <400> 898 gtatcgaata taatttctga acatgaatac c 31 <210> 899 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK12_089 <400> 899 ggtatcgaat ataatttctg aacatgaata ct 32 <210> 900 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK12_089 <400> 900 gataggcagg tccatggata gagtt 25 <210> 901 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK12_096 <400> 901 gtgatggccc agtccagcaa g 21 <210> 902 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK12_096 <400> 902 gtgatggccc agtccagcaa a 21 <210> 903 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK12_096 <400> 903 gggctagttt tattctctta atgcacctt 29 <210> 904 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK12_110 <400> 904 cagtttcaat catgtcactt ttccca 26 <210> 905 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK12_110 <400> 905 cagtttcaat catgtcactt ttcccg 26 <210> 906 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK12_110 <400> 906 catattccag cacctatcct gtcaataat 29 <210> 907 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK12_118 <400> 907 gacatcctaa acccttttct ttataaactt 30 <210> 908 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK12_118 <400> 908 acatcctaaa cccttttctt tataaactc 29 <210> 909 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK12_118 <400> 909 gtgtttggtt ttcctttgtt taaccagttt 30 <210> 910 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK12_123 <400> 910 ggccgccttc ctcaatgata gt 22 <210> 911 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK12_123 <400> 911 gccgccttcc tcaatgatag c 21 <210> 912 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK12_123 <400> 912 tgagtagctt ggctgggatt gagat 25 <210> 913 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK12_128 <400> 913 ctgatgcgag tagattctgc c 21 <210> 914 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK12_128 <400> 914 ctctgatgcg agtagattct gca 23 <210> 915 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK12_128 <400> 915 ctgtccttat ccgtcatgta ctaatgatt 29 <210> 916 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK12_134 <400> 916 ttatacctat aattgttaat gattgatatc c 31 <210> 917 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK12_134 <400> 917 ctttatacct ataattgtta atgattgata tct 33 <210> 918 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK12_134 <400> 918 cgagtctcaa ccagatcaat gccaa 25 <210> 919 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK12_138 <400> 919 caggcatcgt tctgtaaggc g 21 <210> 920 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK12_138 <400> 920 ctcaggcatc gttctgtaag gca 23 <210> 921 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK12_138 <400> 921 accgttggca tttgctgcca tatttattt 29 <210> 922 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK12_147 <400> 922 cggagttgct acccaatgca gt 22 <210> 923 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK12_147 <400> 923 ggagttgcta cccaatgcag g 21 <210> 924 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK12_147 <400> 924 gcgctgtgtt atcctcagct aacat 25 <210> 925 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK12_151 <400> 925 aacaaaaagg gaaccaaaaa gcatgac 27 <210> 926 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK12_151 <400> 926 caacaaaaag ggaaccaaaa agcatgat 28 <210> 927 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK12_151 <400> 927 ctctcagaat ccaatttcac agccttaat 29 <210> 928 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK12_161 <400> 928 caaagcgacg gaggctttga c 21 <210> 929 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK12_161 <400> 929 gcaaagcgac ggaggctttg at 22 <210> 930 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK12_161 <400> 930 gaatcggccc tcagggctct aa 22 <210> 931 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK13_003 <400> 931 caatgaaatt taagaagttg tgaatggcc 29 <210> 932 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK13_003 <400> 932 caatgaaatt taagaagttg tgaatggca 29 <210> 933 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK13_003 <400> 933 caccctcaat ctcatccagc tttgtt 26 <210> 934 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK13_008 <400> 934 cattgccttg gtcaaacaaa taatatt 27 <210> 935 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK13_008 <400> 935 ctcattgcct tggtcaaaca aataatatg 29 <210> 936 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK13_008 <400> 936 caaagcaatg aacatgaaaa gtgtcctata 30 <210> 937 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK13_015 <400> 937 cccaagtctc gagaagagga g 21 <210> 938 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK13_015 <400> 938 tcccaagtct cgagaagagg aa 22 <210> 939 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK13_015 <400> 939 ctccaatcgg ctttgctcgt cttt 24 <210> 940 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK13_023 <400> 940 gtgcaaacac ctaagctctc ctc 23 <210> 941 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK13_023 <400> 941 gtgcaaacac ctaagctctc ctg 23 <210> 942 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK13_023 <400> 942 tctgtcggaa gcagggctta gata 24 <210> 943 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK13_055 <400> 943 agatatattt gttagacgaa agcaaacatc 30 <210> 944 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK13_055 <400> 944 atagatatat ttgttagacg aaagcaaaca tt 32 <210> 945 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK13_055 <400> 945 gaccactctt gttgttttga aaaactacat 30 <210> 946 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK13_061 <400> 946 ccaaattatt ctcatacgtc tttccatg 28 <210> 947 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK13_061 <400> 947 aaccaaatta ttctcatacg tctttccata 30 <210> 948 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK13_061 <400> 948 cgtaacttgt tattattatc tattcgtgaa 30 <210> 949 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK13_067 <400> 949 aaaaatgatc attggttaat taagtacgag aa 32 <210> 950 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK13_067 <400> 950 aaatgatcat tggttaatta agtacgagag 30 <210> 951 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK13_067 <400> 951 caccaactct gaactgtttt tgtgatgat 29 <210> 952 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK13_071 <400> 952 attatttata attactccaa agttgctctt ctt 33 <210> 953 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK13_071 <400> 953 atttataatt actccaaagt tgctcttcta 30 <210> 954 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK13_071 <400> 954 ccgcacagac gattatgagc tgaaa 25 <210> 955 <211> 28 <212> DNA <213> artificial sequence <220> <223> SK13_080 <400> 955 cacataagga ctaaaactga gattagga 28 <210> 956 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK13_080 <400> 956 acataaggac taaaactgag attaggg 27 <210> 957 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK13_080 <400> 957 cacagaacaa tcccttttgc actctaaaa 29 <210> 958 <211> 32 <212> DNA <213> artificial sequence <220> <223> SK13_088 <400> 958 ttatataagg aaaaaaatcc aattcattat ca 32 <210> 959 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK13_088 <400> 959 tatataagga aaaaaatcca attcattatc g 31 <210> 960 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK13_088 <400> 960 ggctgcaaat caaaaccagg aaattcata 29 <210> 961 <211> 27 <212> DNA <213> artificial sequence <220> <223> SK13_094 <400> 961 acttactgtc aaactggtta agggaaa 27 <210> 962 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK13_094 <400> 962 cttactgtca aactggttaa gggaag 26 <210> 963 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK13_094 <400> 963 gacgtagtga ttctagtaat tcctgcaat 29 <210> 964 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK13_103 <400> 964 gcagtcagaa atgggagaat ctgat 25 <210> 965 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK13_103 <400> 965 cagtcagaaa tgggagaatc tgag 24 <210> 966 <211> 19 <212> DNA <213> artificial sequence <220> <223> SK13_103 <400> 966 gggctgcggc gctgggaat 19 <210> 967 <211> 20 <212> DNA <213> artificial sequence <220> <223> SK13_107 <400> 967 cctatctggt gaggagtctc 20 <210> 968 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK13_107 <400> 968 gctcctatct ggtgaggagt ctt 23 <210> 969 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK13_107 <400> 969 tcagagggat gtactccgta gctt 24 <210> 970 <211> 21 <212> DNA <213> artificial sequence <220> <223> SK13_113 <400> 970 ttgacgactg attggcgagg g 21 <210> 971 <211> 20 <212> DNA <213> artificial sequence <220> <223> SK13_113 <400> 971 tgacgactga ttggcgaggc 20 <210> 972 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK13_113 <400> 972 aaaacagata acctgatttc tccagcaaaa 30 <210> 973 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK13_123 <400> 973 aaagtttggg tgtttgagcc tcc 23 <210> 974 <211> 24 <212> DNA <213> artificial sequence <220> <223> SK13_123 <400> 974 gaaagtttgg gtgtttgagc ctct 24 <210> 975 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK13_123 <400> 975 gatgtgcaca ggagacccaa catat 25 <210> 976 <211> 22 <212> DNA <213> artificial sequence <220> <223> SK13_127 <400> 976 gtctgtggtt agcctctggg aa 22 <210> 977 <211> 20 <212> DNA <213> artificial sequence <220> <223> SK13_127 <400> 977 ctgtggttag cctctgggag 20 <210> 978 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK13_127 <400> 978 caaagctaag aagtacaaaa catcacattt 30 <210> 979 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK13_131 <400> 979 agttggtcgc ttcaactgct aaaac 25 <210> 980 <211> 26 <212> DNA <213> artificial sequence <220> <223> SK13_131 <400> 980 gagttggtcg cttcaactgc taaaat 26 <210> 981 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK13_131 <400> 981 ccagagagtg ttttttcagt gtgatcata 29 <210> 982 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK13_138 <400> 982 atgggcgtta cctcacaaga aagat 25 <210> 983 <211> 23 <212> DNA <213> artificial sequence <220> <223> SK13_138 <400> 983 gggcgttacc tcacaagaaa gag 23 <210> 984 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK13_138 <400> 984 cgggtgcaag ctatcagcta tgata 25 <210> 985 <211> 31 <212> DNA <213> artificial sequence <220> <223> SK13_150 <400> 985 agtccatgca catttaaata tacaaaatct a 31 <210> 986 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK13_150 <400> 986 gtccatgcac atttaaatat acaaaatctc 30 <210> 987 <211> 25 <212> DNA <213> artificial sequence <220> <223> SK13_150 <400> 987 ccccacttag tctccttgtt gagaa 25 <210> 988 <211> 29 <212> DNA <213> artificial sequence <220> <223> SK13_157 <400> 988 attggaccaa aattgctact attcaaaac 29 <210> 989 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK13_157 <400> 989 atatattgga ccaaaattgc tactattcaa aat 33 <210> 990 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK13_157 <400> 990 tccaatacta ctagtagcta taaatgccaa 30 <210> 991 <211> 33 <212> DNA <213> artificial sequence <220> <223> SK13_164 <400> 991 atttttttct taaaatgcca aagtcactat ttg 33 <210> 992 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK13_164 <400> 992 tttttcttaa aatgccaaag tcactattta 30 <210> 993 <211> 30 <212> DNA <213> artificial sequence <220> <223> SK13_164 <400> 993 gctcgatgaa caaacgaaac tagaattaaa 30

Claims (9)

The first allele-specific forward primer represented by SEQ ID NO: 1 for SK01_007, the second allele-specific forward primer represented by SEQ ID NO: 2, and the reverse primer set represented by SEQ ID NO: 3, SEQ ID NO: 4 for SK01_012 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 5 and the reverse primer set represented by SEQ ID NO: 6, the first allele-specific forward primer represented by SEQ ID NO: 7 for SK01_018, sequence The second allele-specific forward primer represented by No. 8 and the reverse primer set represented by SEQ ID NO: 9, the first allele-specific forward primer represented by SEQ ID NO: 10 for SK01_025, the second allele represented by SEQ ID NO: 11 A set of specific forward primers and reverse primers represented by SEQ ID NO: 12, a first allele-specific forward primer represented by SEQ ID NO: 13 for SK01_030, a second allele-specific forward primer represented by SEQ ID NO: 14, and SEQ ID NO: 15 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 16 for SK01_038, a second allele-specific forward primer represented by SEQ ID NO: 17, and a reverse primer set represented by SEQ ID NO: 18, sequence for SK01_046 The first allele-specific forward primer represented by SEQ ID NO: 19, the second allele-specific forward primer represented by SEQ ID NO: 20, and the reverse primer set represented by SEQ ID NO: 21, the first allele represented by SEQ ID NO: 22 for SK01_054 A specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 23 and a reverse primer set represented by SEQ ID NO: 24, a first allele-specific forward primer represented by SEQ ID NO: 25 for SKJ01_070, represented by SEQ ID NO: 26 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 27, a first allele-specific forward primer represented by SEQ ID NO: 28 for SK01_084, a second allele-specific forward primer represented by SEQ ID NO: 29, and A reverse primer set represented by SEQ ID NO: 30, a first allele-specific forward primer represented by SEQ ID NO: 31 for SK01_093, a second allele-specific forward primer represented by SEQ ID NO: 32, and a reverse primer set represented by SEQ ID NO: 33 , the first allele-specific forward primer represented by SEQ ID NO: 34 for SK01_102, the second allele-specific forward primer represented by SEQ ID NO: 35 and the reverse primer set represented by SEQ ID NO: 36, SEQ ID NO: 37 for SK01_109 A set of first allele-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 38 and a reverse primer set represented by SEQ ID NO: 39, the first allele-specific forward primer represented by SEQ ID NO: 40 for SK01_113 , the second allele-specific forward primer represented by SEQ ID NO: 41 and the reverse primer set represented by SEQ ID NO: 42, the first allele-specific forward primer represented by SEQ ID NO: 43 for SK01_120, the second allele-specific forward primer represented by SEQ ID NO: 44 Allele-specific forward primer and reverse primer set represented by SEQ ID NO: 45, first allele-specific forward primer represented by SEQ ID NO: 46 for SK01_128, second allele-specific forward primer represented by SEQ ID NO: 47 and SEQ ID NO: 48 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 49 for SK01_133, a second allele-specific forward primer represented by SEQ ID NO: 50, and a reverse primer set represented by SEQ ID NO: 51, to SK01_139 A set of a first allele-specific forward primer represented by SEQ ID NO: 52, a second allele-specific forward primer represented by SEQ ID NO: 53, and a reverse primer set represented by SEQ ID NO: 54, a first set represented by SEQ ID NO: 55 for SK01_144 An allele-specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 56 and a set of reverse primers represented by SEQ ID NO: 57, a first allele-specific forward primer represented by SEQ ID NO: 58 for SK01_152, SEQ ID NO: 59 A second allele-specific forward primer represented by and a reverse primer set represented by SEQ ID NO: 60, a first allele-specific forward primer represented by SEQ ID NO: 61 for SK01_160, and a second allele-specific forward primer represented by SEQ ID NO: 62 A set of primers and reverse primers represented by SEQ ID NO: 63, a first allele-specific forward primer represented by SEQ ID NO: 64 for SK01_174, a second allele-specific forward primer represented by SEQ ID NO: 65, and a reverse primer represented by SEQ ID NO: 66 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 67 for SK01_181, a second allele-specific forward primer represented by SEQ ID NO: 68, and a reverse primer set represented by SEQ ID NO: 69, SEQ ID NO: 70 for SK01_187 The first allele-specific forward primer represented by SEQ ID NO: 71, the second allele-specific forward primer represented by SEQ ID NO: 72, and the reverse primer set represented by SEQ ID NO: 72, the first allele-specific forward primer represented by SEQ ID NO: 73 for SK01_194 Primers, the second allele-specific forward primer represented by SEQ ID NO: 74 and the reverse primer set represented by SEQ ID NO: 75, the first allele-specific forward primer represented by SEQ ID NO: 76 for SK01_201, the first allele-specific forward primer represented by SEQ ID NO: 77 2 Allele-specific forward primer and reverse primer set represented by SEQ ID NO: 78, the first allele-specific forward primer represented by SEQ ID NO: 79 for SK02_007, the second allele-specific forward primer represented by SEQ ID NO: 80 and SEQ ID NO: A reverse primer set represented by SEQ ID NO: 81, a first allele-specific forward primer represented by SEQ ID NO: 82 for SK02_013, a second allele-specific forward primer represented by SEQ ID NO: 83, and a reverse primer set represented by SEQ ID NO: 84, SK02_022 The first allele-specific forward primer represented by SEQ ID NO: 85, the second allele-specific forward primer represented by SEQ ID NO: 86, and the reverse primer set represented by SEQ ID NO: 87, SEQ ID NO: 88 for SK02_027 The first allele specific forward primer, the second allele specific forward primer represented by SEQ ID NO: 89 and the reverse primer set represented by SEQ ID NO: 90, the first allele specific forward primer represented by SEQ ID NO: 91 for SK02-036 Primers, the second allele-specific forward primer represented by SEQ ID NO: 92 and the reverse primer set represented by SEQ ID NO: 93, the first allele-specific forward primer represented by SEQ ID NO: 94 for SK02_041, SEQ ID NO: 95 A second allele-specific forward primer and a set of reverse primers represented by SEQ ID NO: 96, a first allele-specific forward primer represented by SEQ ID NO: 97 for SK02_050, a second allele-specific forward primer represented by SEQ ID NO: 98, and A reverse primer set represented by SEQ ID NO: 99, a first allele-specific forward primer represented by SEQ ID NO: 100 for SK02_059, a second allele-specific forward primer represented by SEQ ID NO: 101, and a reverse primer represented by SEQ ID NO: 102 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 103 for SK02_070, a second allele-specific forward primer represented by SEQ ID NO: 104, and a reverse primer set represented by SEQ ID NO: 105, SEQ ID NO: SK02_076 The first allele-specific forward primer represented by SEQ ID NO: 106, the second allele-specific forward primer represented by SEQ ID NO: 107, and the reverse primer set represented by SEQ ID NO: 108, the first allele represented by SEQ ID NO: 109 for SK02_081 A specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 110 and a set of reverse primers represented by SEQ ID NO: 111, a first allele-specific forward primer represented by SEQ ID NO: 112 for SK02_087, SEQ ID NO: 113 The second allele-specific forward primer and the reverse primer set represented by SEQ ID NO: 114, the first allele-specific forward primer represented by SEQ ID NO: 115 for SK02_089, and the second allele-specific forward primer represented by SEQ ID NO: 116 Primers and a reverse primer set represented by SEQ ID NO: 117, a first allele-specific forward primer represented by SEQ ID NO: 118 for SK02_097, a second allele-specific forward primer represented by SEQ ID NO: 119, and SEQ ID NO: 120 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 121 for SK02_103, a second allele-specific forward primer represented by SEQ ID NO: 122, and a reverse primer set represented by SEQ ID NO: 123, for SK02_110 A set of first allele-specific forward primers represented by SEQ ID NO: 124, second allele-specific forward primers represented by SEQ ID NO: 125 and reverse primers represented by SEQ ID NO: 126, the first set represented by SEQ ID NO: 127 for SK02_117 Allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 128 and the reverse primer set represented by SEQ ID NO: 129, the first allele-specific forward primer represented by SEQ ID NO: 130 for SK02_121, SEQ ID NO: The second allele-specific forward primer represented by SEQ ID NO: 131 and the reverse primer set represented by SEQ ID NO: 132, the first allele-specific forward primer represented by SEQ ID NO: 133 for SK02_133, the second allele represented by SEQ ID NO: 134 A specific forward primer and a set of reverse primers represented by SEQ ID NO: 135, a first allele-specific forward primer represented by SEQ ID NO: 136 for SK02_140, a second allele-specific forward primer represented by SEQ ID NO: 137, and SEQ ID NO: 138 The reverse primer set represented by, the first allele-specific forward primer represented by SEQ ID NO: 139 for SK02_151, the second allele-specific forward primer represented by SEQ ID NO: 140, and the reverse primer set represented by SEQ ID NO: 141, SK02_160 The first allele-specific forward primer represented by SEQ ID NO: 142, the second allele-specific forward primer represented by SEQ ID NO: 143, and the reverse primer set represented by SEQ ID NO: 144, SEQ ID NO: 145 for SK02_167 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 146 and the reverse primer set represented by SEQ ID NO: 147, the first allele-specific forward primer represented by SEQ ID NO: 148 for SK02_179, The second allele-specific forward primer represented by SEQ ID NO: 149 and the reverse primer set represented by SEQ ID NO: 150, the first allele-specific forward primer represented by SEQ ID NO: 151 for SK03_003, and the second allele-specific forward primer represented by SEQ ID NO: 152 Allele-specific forward primer and reverse primer set represented by SEQ ID NO: 153, the first allele-specific forward primer represented by SEQ ID NO: 154 for SK03_012, the second allele-specific forward primer represented by SEQ ID NO: 155, and SEQ ID NO: A reverse primer set represented by SEQ ID NO: 156, a first allele-specific forward primer represented by SEQ ID NO: 157 for SK03_020, a second allele-specific forward primer represented by SEQ ID NO: 158, and a reverse primer set represented by SEQ ID NO: 159 , the first allele-specific forward primer represented by SEQ ID NO: 160 for SK03_029, the second allele-specific forward primer represented by SEQ ID NO: 161 and the reverse primer set represented by SEQ ID NO: 162, SEQ ID NO: 163 for SK03_030. The first allele-specific forward primer represented by SEQ ID NO: 164, the second allele-specific forward primer represented by SEQ ID NO: 165, and the reverse primer set represented by SEQ ID NO: 165, the first allele-specific forward primer represented by SEQ ID NO: 166 for SK03_038 Primers, the second allele-specific forward primer represented by SEQ ID NO: 167 and the reverse primer set represented by SEQ ID NO: 168, the first allele-specific forward primer represented by SEQ ID NO: 169 for SK03_044, SEQ ID NO: 170 A second allele-specific forward primer and a set of reverse primers represented by SEQ ID NO: 171, a first allele-specific forward primer represented by SEQ ID NO: 172 for SK03_050, a second allele-specific forward primer represented by SEQ ID NO: 173, and A reverse primer set represented by SEQ ID NO: 174, a first allele-specific forward primer represented by SEQ ID NO: 175 for SK03_055, a second allele-specific forward primer represented by SEQ ID NO: 176, and a reverse primer represented by SEQ ID NO: 177 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 178 for SK03_063, a second allele-specific forward primer represented by SEQ ID NO: 179, and a reverse primer set represented by SEQ ID NO: 180, SEQ ID NO: SK03_070 The first allele-specific forward primer represented by SEQ ID NO: 181, the second allele-specific forward primer represented by SEQ ID NO: 182, and the reverse primer set represented by SEQ ID NO: 183, the first allele represented by SEQ ID NO: 184 for SK03_079 A specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 185 and a set of reverse primers represented by SEQ ID NO: 186, a first allele-specific forward primer represented by SEQ ID NO: 187 for SK03_094, SEQ ID NO: 188 The second allele-specific forward primer and the reverse primer set represented by SEQ ID NO: 189, the first allele-specific forward primer represented by SEQ ID NO: 190 for SK03_098, and the second allele-specific forward primer represented by SEQ ID NO: 191 Primers and a set of reverse primers represented by SEQ ID NO: 192, a first allele-specific forward primer represented by SEQ ID NO: 193 for SK03_102, a second allele-specific forward primer represented by SEQ ID NO: 194, and SEQ ID NO: 195 The reverse primer set, the first allele-specific forward primer represented by SEQ ID NO: 196 for SK03_108, the second allele-specific forward primer represented by SEQ ID NO: 197, and the reverse primer set represented by SEQ ID NO: 198, for SK03_116 A set of first allele-specific forward primers represented by SEQ ID NO: 199, second allele-specific forward primers represented by SEQ ID NO: 200 and reverse primers represented by SEQ ID NO: 201, the first set represented by SEQ ID NO: 202 for SK03_120 Allele-specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 203 and a set of reverse primers represented by SEQ ID NO: 204, a first allele-specific forward primer represented by SEQ ID NO: 205 for SK03_126, SEQ ID NO: The second allele-specific forward primer represented by SEQ ID NO: 206 and the reverse primer set represented by SEQ ID NO: 207, the first allele-specific forward primer represented by SEQ ID NO: 208 for SK03_141, the second allele represented by SEQ ID NO: 209 A specific forward primer and a set of reverse primers represented by SEQ ID NO: 210, a first allele-specific forward primer represented by SEQ ID NO: 211 for SK03_147, a second allele-specific forward primer represented by SEQ ID NO: 212, and SEQ ID NO: 213 The reverse primer set represented by, the first allele-specific forward primer represented by SEQ ID NO: 214 for SK03_151, the second allele-specific forward primer represented by SEQ ID NO: 215, and the reverse primer set represented by SEQ ID NO: 216, SK03_158 The first allele-specific forward primer represented by SEQ ID NO: 217, the second allele-specific forward primer represented by SEQ ID NO: 218, and the reverse primer set represented by SEQ ID NO: 219, SEQ ID NO: 220 for SK03_166 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 221 and the reverse primer set represented by SEQ ID NO: 222, the first allele-specific forward primer represented by SEQ ID NO: 223 for SK03_173, The second allele-specific forward primer represented by SEQ ID NO: 224 and the reverse primer set represented by SEQ ID NO: 225, the first allele-specific forward primer represented by SEQ ID NO: 226 for SK03_180, the second allele-specific forward primer represented by SEQ ID NO: 227 Allele-specific forward primer and reverse primer set represented by SEQ ID NO: 228, the first allele-specific forward primer represented by SEQ ID NO: 229 for SK03_191, the second allele-specific forward primer represented by SEQ ID NO: 230, and SEQ ID NO: A reverse primer set represented by SEQ ID NO: 231, a first allele-specific forward primer represented by SEQ ID NO: 232 for SK03_203, a second allele-specific forward primer represented by SEQ ID NO: 233, and a reverse primer set represented by SEQ ID NO: 234 , the first allele-specific forward primer represented by SEQ ID NO: 235 for SK03_219, the second allele-specific forward primer represented by SEQ ID NO: 236 and the reverse primer set represented by SEQ ID NO: 237, SEQ ID NO: 238 for SK03_226 The first allele-specific forward primer represented by SEQ ID NO: 239, the second allele-specific forward primer represented by SEQ ID NO: 240, and the set of reverse primers represented by SEQ ID NO: 240, the first allele-specific forward primer represented by SEQ ID NO: 241 for SK03_237 Primers, the second allele-specific forward primer represented by SEQ ID NO: 242 and the reverse primer set represented by SEQ ID NO: 243, the first allele-specific forward primer represented by SEQ ID NO: 244 for SK03_245, SEQ ID NO: 245 A second allele-specific forward primer and a set of reverse primers represented by SEQ ID NO: 246, a first allele-specific forward primer represented by SEQ ID NO: 247 for SK03_252, a second allele-specific forward primer represented by SEQ ID NO: 248, and A reverse primer set represented by SEQ ID NO: 249, a first allele-specific forward primer represented by SEQ ID NO: 250 for SK03_258, a second allele-specific forward primer represented by SEQ ID NO: 251, and a reverse primer represented by SEQ ID NO: 252 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 253 for SK04_008, a second allele-specific forward primer represented by SEQ ID NO: 254, and a reverse primer set represented by SEQ ID NO: 255, SEQ ID NO: 255 The first allele-specific forward primer represented by SEQ ID NO: 256, the second allele-specific forward primer represented by SEQ ID NO: 257, and the reverse primer set represented by SEQ ID NO: 258, the first allele represented by SEQ ID NO: 259 for SK04_023 A specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 260 and a set of reverse primers represented by SEQ ID NO: 261, a first allele-specific forward primer represented by SEQ ID NO: 262 for SK04_029, SEQ ID NO: 263 The second allele-specific forward primer and the reverse primer set represented by SEQ ID NO: 264, the first allele-specific forward primer represented by SEQ ID NO: 265 for SK04_045, and the second allele-specific forward primer represented by SEQ ID NO: 266 Primers and a set of reverse primers represented by SEQ ID NO: 267, a first allele-specific forward primer represented by SEQ ID NO: 268 for SK04_051, a second allele-specific forward primer represented by SEQ ID NO: 269, and SEQ ID NO: 270 The reverse primer set, the first allele-specific forward primer represented by SEQ ID NO: 271 for SK04_060, the second allele-specific forward primer represented by SEQ ID NO: 272, and the reverse primer set represented by SEQ ID NO: 273, for SK04_069 A set of first allele-specific forward primers represented by SEQ ID NO: 274, second allele-specific forward primers represented by SEQ ID NO: 275 and reverse primers represented by SEQ ID NO: 276, the first set represented by SEQ ID NO: 277 for SK04_080 Allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 278 and the set of reverse primers represented by SEQ ID NO: 279, the first allele-specific forward primer represented by SEQ ID NO: 280 for SK04_090, SEQ ID NO: The second allele-specific forward primer represented by SEQ ID NO: 281 and the reverse primer set represented by SEQ ID NO: 282, the first allele-specific forward primer represented by SEQ ID NO: 283 for SK04_098, the second allele represented by SEQ ID NO: 284 A specific forward primer and a set of reverse primers represented by SEQ ID NO: 285, a first allele-specific forward primer represented by SEQ ID NO: 286 for SK04_107, a second allele-specific forward primer represented by SEQ ID NO: 287, and SEQ ID NO: 288 The reverse primer set represented by, the first allele-specific forward primer represented by SEQ ID NO: 289 for SK04_114, the second allele-specific forward primer represented by SEQ ID NO: 290, and the reverse primer set represented by SEQ ID NO: 291, SK04_122 The first allele-specific forward primer represented by SEQ ID NO: 292, the second allele-specific forward primer represented by SEQ ID NO: 293, and the reverse primer set represented by SEQ ID NO: 294, SEQ ID NO: 295 for SK04_129 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 296 and the reverse primer set represented by SEQ ID NO: 297, the first allele-specific forward primer represented by SEQ ID NO: 298 for SK04_138, The second allele-specific forward primer represented by SEQ ID NO: 299 and the reverse primer set represented by SEQ ID NO: 300, the first allele-specific forward primer represented by SEQ ID NO: 301 for SK04_143, and the second allele-specific forward primer represented by SEQ ID NO: 302 Allele-specific forward primer and reverse primer set represented by SEQ ID NO: 303, first allele-specific forward primer represented by SEQ ID NO: 304 for SK04_149, second allele-specific forward primer represented by SEQ ID NO: 305 and SEQ ID NO: A reverse primer set represented by SEQ ID NO: 306, a first allele-specific forward primer represented by SEQ ID NO: 307 for SK04_158, a second allele-specific forward primer represented by SEQ ID NO: 308, and a reverse primer set represented by SEQ ID NO: 309 , the first allele-specific forward primer represented by SEQ ID NO: 310 for SK04_164, the second allele-specific forward primer represented by SEQ ID NO: 311 and the reverse primer set represented by SEQ ID NO: 312, SEQ ID NO: 313 for SK04_174 The first allele-specific forward primer represented by SEQ ID NO: 314, the second allele-specific forward primer represented by SEQ ID NO: 315, and the set of reverse primers represented by SEQ ID NO: 315, the first allele-specific forward primer represented by SEQ ID NO: 316 for SK04_180 Primers, the second allele-specific forward primer represented by SEQ ID NO: 317 and the reverse primer set represented by SEQ ID NO: 318, the first allele-specific forward primer represented by SEQ ID NO: 319 for SK04_183, SEQ ID NO: 320 A second allele-specific forward primer and a set of reverse primers represented by SEQ ID NO: 321, a first allele-specific forward primer represented by SEQ ID NO: 322 for SK04_189, a second allele-specific forward primer represented by SEQ ID NO: 323, and A reverse primer set represented by SEQ ID NO: 324, a first allele-specific forward primer represented by SEQ ID NO: 325 for SK04_195, a second allele-specific forward primer represented by SEQ ID NO: 326, and a reverse primer represented by SEQ ID NO: 327 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 328 for SK04_200, a second allele-specific forward primer represented by SEQ ID NO: 329, and a reverse primer set represented by SEQ ID NO: 330, SEQ ID NO: The first allele-specific forward primer represented by SEQ ID NO: 331, the second allele-specific forward primer represented by SEQ ID NO: 332, and the reverse primer set represented by SEQ ID NO: 333, the first allele represented by SEQ ID NO: 334 for SK05_003 A specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 335 and a set of reverse primers represented by SEQ ID NO: 336, a first allele-specific forward primer represented by SEQ ID NO: 337 for SK05_010, SEQ ID NO: 338 The second allele-specific forward primer and the reverse primer set represented by SEQ ID NO: 339, the first allele-specific forward primer represented by SEQ ID NO: 340 for SK05_015, and the second allele-specific forward primer represented by SEQ ID NO: 341 Primers and a set of reverse primers represented by SEQ ID NO: 342, a first allele-specific forward primer represented by SEQ ID NO: 343 for SK05_017, a second allele-specific forward primer represented by SEQ ID NO: 344, and SEQ ID NO: 345 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 346 for SK05_032, a second allele-specific forward primer represented by SEQ ID NO: 347, and a reverse primer set represented by SEQ ID NO: 348, for SK05_036 A set of first allele-specific forward primers represented by SEQ ID NO: 349, second allele-specific forward primers represented by SEQ ID NO: 350 and reverse primers represented by SEQ ID NO: 351, the first set represented by SEQ ID NO: 352 for SK05_039 Allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 353 and the set of reverse primers represented by SEQ ID NO: 354, the first allele-specific forward primer represented by SEQ ID NO: 355 for SK05_059, SEQ ID NO: The second allele-specific forward primer represented by SEQ ID NO: 356 and the reverse primer set represented by SEQ ID NO: 357, the first allele-specific forward primer represented by SEQ ID NO: 358 for SK05_068, the second allele represented by SEQ ID NO: 359 A set of specific forward primers and reverse primers represented by SEQ ID NO: 360, a first allele-specific forward primer represented by SEQ ID NO: 361 for SK05_075, a second allele-specific forward primer represented by SEQ ID NO: 362, and SEQ ID NO: 363. The reverse primer set represented by, the first allele-specific forward primer represented by SEQ ID NO: 364 for SK05_087, the second allele-specific forward primer represented by SEQ ID NO: 365, and the reverse primer set represented by SEQ ID NO: 366, SK05_092 The first allele-specific forward primer represented by SEQ ID NO: 367, the second allele-specific forward primer represented by SEQ ID NO: 368, and the reverse primer set represented by SEQ ID NO: 369, SEQ ID NO: 370 for SK05_100 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 371 and the reverse primer set represented by SEQ ID NO: 372, the first allele-specific forward primer represented by SEQ ID NO: 373 for SK05_108, The second allele-specific forward primer represented by SEQ ID NO: 374 and the reverse primer set represented by SEQ ID NO: 375, the first allele-specific forward primer represented by SEQ ID NO: 374 for SK05_112, the second allele-specific forward primer represented by SEQ ID NO: 375 Allele-specific forward primer and reverse primer set represented by SEQ ID NO: 376, first allele-specific forward primer represented by SEQ ID NO: 379 for SK05_115, second allele-specific forward primer represented by SEQ ID NO: 380 and SEQ ID NO: A reverse primer set represented by 381, a first allele-specific forward primer represented by SEQ ID NO: 382 for SK05_122, a second allele-specific forward primer represented by SEQ ID NO: 383, and a reverse primer set represented by SEQ ID NO: 384 , the first allele-specific forward primer represented by SEQ ID NO: 385 for SK05_132, the second allele-specific forward primer represented by SEQ ID NO: 386 and the reverse primer set represented by SEQ ID NO: 387, SEQ ID NO: 388 for SK05_137 The first allele-specific forward primer represented by SEQ ID NO: 389, the second allele-specific forward primer represented by SEQ ID NO: 390, and the set of reverse primers represented by SEQ ID NO: 390, the first allele-specific forward primer represented by SEQ ID NO: 391 for SK05_148 Primers, the second allele-specific forward primer represented by SEQ ID NO: 392 and the reverse primer set represented by SEQ ID NO: 393, the first allele-specific forward primer represented by SEQ ID NO: 394 for SK05_155, SEQ ID NO: 395 A second allele-specific forward primer and a set of reverse primers represented by SEQ ID NO: 396, a first allele-specific forward primer represented by SEQ ID NO: 397 for SK05_162, a second allele-specific forward primer represented by SEQ ID NO: 398, and A reverse primer set represented by SEQ ID NO: 399, a first allele-specific forward primer represented by SEQ ID NO: 400 for SK06_006, a second allele-specific forward primer represented by SEQ ID NO: 401, and a reverse primer represented by SEQ ID NO: 402 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 403 for SK06_013, a second allele-specific forward primer represented by SEQ ID NO: 404, and a reverse primer set represented by SEQ ID NO: 405, SEQ ID NO: SK06_022 The first allele-specific forward primer represented by SEQ ID NO: 406, the second allele-specific forward primer represented by SEQ ID NO: 407, and the reverse primer set represented by SEQ ID NO: 408, the first allele represented by SEQ ID NO: 409 for SK06_029 A specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 410 and a set of reverse primers represented by SEQ ID NO: 411, a first allele-specific forward primer represented by SEQ ID NO: 412 for SK06_035, SEQ ID NO: 413 The second allele-specific forward primer and the reverse primer set represented by SEQ ID NO: 414, the first allele-specific forward primer represented by SEQ ID NO: 415 for SK06_042, and the second allele-specific forward primer represented by SEQ ID NO: 416 Primers and a set of reverse primers represented by SEQ ID NO: 417, a first allele-specific forward primer represented by SEQ ID NO: 418 for SK06_048, a second allele-specific forward primer represented by SEQ ID NO: 419, and SEQ ID NO: 420 The reverse primer set, the first allele-specific forward primer represented by SEQ ID NO: 421 for SK06_056, the second allele-specific forward primer represented by SEQ ID NO: 422, and the reverse primer set represented by SEQ ID NO: 423, for SK06_063 A set of first allele-specific forward primers represented by SEQ ID NO: 424, second allele-specific forward primers represented by SEQ ID NO: 425, and reverse primers represented by SEQ ID NO: 426, the first set represented by SEQ ID NO: 427 for SK06_067 Allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 428 and the reverse primer set represented by SEQ ID NO: 429, the first allele-specific forward primer represented by SEQ ID NO: 430 for SK06_075, SEQ ID NO: The second allele-specific forward primer represented by 431 and the reverse primer set represented by SEQ ID NO: 432, the first allele-specific forward primer represented by SEQ ID NO: 433 for SK06_081, the second allele represented by SEQ ID NO: 434 A set of specific forward primers and reverse primers represented by SEQ ID NO: 435, a first allele-specific forward primer represented by SEQ ID NO: 436 for SK06_098, a second allele-specific forward primer represented by SEQ ID NO: 437, and SEQ ID NO: 438. The reverse primer set represented by, the first allele-specific forward primer represented by SEQ ID NO: 439 for SK06_105, the second allele-specific forward primer represented by SEQ ID NO: 440, and the reverse primer set represented by SEQ ID NO: 441, SK06_113 The first allele-specific forward primer represented by SEQ ID NO: 442, the second allele-specific forward primer represented by SEQ ID NO: 443, and the reverse primer set represented by SEQ ID NO: 444, SEQ ID NO: 445 for SK06_121 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 446 and the reverse primer set represented by SEQ ID NO: 447, the first allele-specific forward primer represented by SEQ ID NO: 448 for SK06_129, The second allele-specific forward primer represented by SEQ ID NO: 449 and the reverse primer set represented by SEQ ID NO: 450, the first allele-specific forward primer represented by SEQ ID NO: 451 for SK06_136, the second allele-specific forward primer represented by SEQ ID NO: 452 An allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 453, a first allele-specific forward primer represented by SEQ ID NO: 454 for SK06_139, a second allele-specific forward primer represented by SEQ ID NO: 455, and SEQ ID NO: A reverse primer set represented by SEQ ID NO: 456, a first allele-specific forward primer represented by SEQ ID NO: 457 for SK06_145, a second allele-specific forward primer represented by SEQ ID NO: 458, and a reverse primer set represented by SEQ ID NO: 459 , the first allele-specific forward primer represented by SEQ ID NO: 460 for SK06_152, the second allele-specific forward primer represented by SEQ ID NO: 461 and the reverse primer set represented by SEQ ID NO: 462, SEQ ID NO: 463 for SK06_157 The first allele-specific forward primer represented by SEQ ID NO: 464, the second allele-specific forward primer represented by SEQ ID NO: 465, and the set of reverse primers represented by SEQ ID NO: 465, the first allele-specific forward primer represented by SEQ ID NO: 466 for SK06_162 Primers, the second allele-specific forward primer represented by SEQ ID NO: 467 and the reverse primer set represented by SEQ ID NO: 468, the first allele-specific forward primer represented by SEQ ID NO: 469 for SK06_170, SEQ ID NO: 470 A second allele-specific forward primer and a set of reverse primers represented by SEQ ID NO: 471, a first allele-specific forward primer represented by SEQ ID NO: 472 for SK06_177, a second allele-specific forward primer represented by SEQ ID NO: 473, and A reverse primer set represented by SEQ ID NO: 474, a first allele-specific forward primer represented by SEQ ID NO: 475 for SK06_183, a second allele-specific forward primer represented by SEQ ID NO: 476, and a reverse primer represented by SEQ ID NO: 477 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 478 for SK06_190, a second allele-specific forward primer represented by SEQ ID NO: 479, and a reverse primer set represented by SEQ ID NO: 480, SEQ ID NO: The first allele-specific forward primer represented by SEQ ID NO: 481, the second allele-specific forward primer represented by SEQ ID NO: 482, and the reverse primer set represented by SEQ ID NO: 483, the first allele represented by SEQ ID NO: 484 for SK06_198 A specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 485 and a set of reverse primers represented by SEQ ID NO: 486, a first allele-specific forward primer represented by SEQ ID NO: 487 for SK06_205, SEQ ID NO: 488 The second allele-specific forward primer and the reverse primer set represented by SEQ ID NO: 489, the first allele-specific forward primer represented by SEQ ID NO: 490 for SK06_212, and the second allele-specific forward primer represented by SEQ ID NO: 491 Primers and a set of reverse primers represented by SEQ ID NO: 492, a first allele-specific forward primer represented by SEQ ID NO: 493 for SK06_222, a second allele-specific forward primer represented by SEQ ID NO: 494, and SEQ ID NO: 495 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 496 for SK06_235, a second allele-specific forward primer represented by SEQ ID NO: 497, and a reverse primer set represented by SEQ ID NO: 498, for SK06_240 A set of first allele-specific forward primers represented by SEQ ID NO: 499, second allele-specific forward primers represented by SEQ ID NO: 500 and reverse primers represented by SEQ ID NO: 501, the first set represented by SEQ ID NO: 502 for SK06_249 Allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 503 and the reverse primer set represented by SEQ ID NO: 504, the first allele-specific forward primer represented by SEQ ID NO: 505 for SK06_256, SEQ ID NO: The second allele-specific forward primer represented by SEQ ID NO: 506 and the reverse primer set represented by SEQ ID NO: 507, the first allele-specific forward primer represented by SEQ ID NO: 508 for SK07_006, the second allele represented by SEQ ID NO: 509 A specific forward primer and a set of reverse primers represented by SEQ ID NO: 510, a first allele-specific forward primer represented by SEQ ID NO: 511 for SK07_022, a second allele-specific forward primer represented by SEQ ID NO: 512, and SEQ ID NO: 513 The reverse primer set represented by, the first allele-specific forward primer represented by SEQ ID NO: 514 for SK07_043, the second allele-specific forward primer represented by SEQ ID NO: 515, and the reverse primer set represented by SEQ ID NO: 516, SK07_047 The first allele-specific forward primer represented by SEQ ID NO: 517, the second allele-specific forward primer represented by SEQ ID NO: 518, and the reverse primer set represented by SEQ ID NO: 519, SEQ ID NO: 520 for SK07_055 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 521 and the reverse primer set represented by SEQ ID NO: 522, the first allele-specific forward primer represented by SEQ ID NO: 523 for SK07_064, The second allele-specific forward primer represented by SEQ ID NO: 524 and the reverse primer set represented by SEQ ID NO: 525, the first allele-specific forward primer represented by SEQ ID NO: 526 for SK07_073, the second allele-specific forward primer represented by SEQ ID NO: 527 An allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 528, a first allele-specific forward primer represented by SEQ ID NO: 529 for SK07_079, a second allele-specific forward primer represented by SEQ ID NO: 530, and SEQ ID NO: A reverse primer set represented by 531, a first allele-specific forward primer represented by SEQ ID NO: 532 for SK07_083, a second allele-specific forward primer represented by SEQ ID NO: 533, and a reverse primer set represented by SEQ ID NO: 534 , the first allele-specific forward primer represented by SEQ ID NO: 535 for SK07_094, the second allele-specific forward primer represented by SEQ ID NO: 536 and the reverse primer set represented by SEQ ID NO: 537, SEQ ID NO: 538 for SK07_100. The first allele-specific forward primer represented by SEQ ID NO: 539, the second allele-specific forward primer represented by SEQ ID NO: 540, and the reverse primer set represented by SEQ ID NO: 541 for SK07_106. Primers, the second allele-specific forward primer represented by SEQ ID NO: 542 and the reverse primer set represented by SEQ ID NO: 543, the first allele-specific forward primer represented by SEQ ID NO: 544 for SK07_114, the first allele-specific forward primer represented by SEQ ID NO: 545 2 allele-specific forward primers and reverse primer set represented by SEQ ID NO: 546, the first allele-specific forward primer represented by SEQ ID NO: 547 for SK07_126, the second allele-specific forward primer represented by SEQ ID NO: 548 and sequence Reverse primer set represented by No. 549, the first allele-specific forward primer represented by SEQ ID NO: 550 for SK07_133, the second allele-specific forward primer represented by SEQ ID NO: 551, and the reverse primer represented by SEQ ID NO: 552 Set, a first allele specific forward primer represented by SEQ ID NO: 553 to SK07_130, a second allele specific forward primer represented by SEQ ID NO: 554 and a reverse primer set represented by SEQ ID NO: 555, SEQ ID NO: 556 to SK07_145 The first allele-specific forward primer represented by SEQ ID NO: 557, the second allele-specific forward primer represented by SEQ ID NO: 558, and the reverse primer set represented by SEQ ID NO: 558, the first allele-specific represented by SEQ ID NO: 559 for SK07_151 Forward primer, a second allele-specific forward primer represented by SEQ ID NO: 560 and a set of reverse primers represented by SEQ ID NO: 561, a first allele-specific forward primer represented by SEQ ID NO: 562 for SK07_164, SEQ ID NO: 563 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 564, a first allele-specific forward primer represented by SEQ ID NO: 565 for SK07_166, and a second allele-specific forward primer represented by SEQ ID NO: 566 and a set of reverse primers represented by SEQ ID NO: 567, a first allele-specific forward primer represented by SEQ ID NO: 568 for SK08_001, a second allele-specific forward primer represented by SEQ ID NO: 569, and a reverse sequence represented by SEQ ID NO: 570. The anti-primer set, the first allele-specific forward primer represented by SEQ ID NO: 571 for SK08_008, the second allele-specific forward primer represented by SEQ ID NO: 572, and the reverse primer set represented by SEQ ID NO: 573, the sequence for SK08_012 The first allele-specific forward primer represented by SEQ ID NO: 574, the second allele-specific forward primer represented by SEQ ID NO: 575, and the reverse primer set represented by SEQ ID NO: 576, the first allele represented by SEQ ID NO: 577 for SK08_020 Trait specific forward primer, second allele specific forward primer represented by SEQ ID NO: 578 and reverse primer set represented by SEQ ID NO: 579, first allele specific forward primer represented by SEQ ID NO: 580 for SK08_032, SEQ ID NO: 581 A second allele-specific forward primer represented by and a reverse primer set represented by SEQ ID NO: 582, a first allele-specific forward primer represented by SEQ ID NO: 583 for SK08_039, and a second allele-specific represented by SEQ ID NO: 584 A forward primer and a reverse primer set represented by SEQ ID NO: 585, a first allele-specific forward primer represented by SEQ ID NO: 586 for SK08_046, a second allele-specific forward primer represented by SEQ ID NO: 587, and SEQ ID NO: 588 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 589 for SK08_052, a second allele-specific forward primer represented by SEQ ID NO: 590, and a reverse primer set represented by SEQ ID NO: 591, SK08_066 The first allele-specific forward primer represented by SEQ ID NO: 592, the second allele-specific forward primer represented by SEQ ID NO: 593, and the reverse primer set represented by SEQ ID NO: 594, the first allele-specific forward primer represented by SEQ ID NO: 595 for SK08_072 1 allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 596 and the reverse primer set represented by SEQ ID NO: 597, the first allele-specific forward primer represented by SEQ ID NO: 598 for SK08_125, sequence The second allele-specific forward primer represented by No. 599 and the reverse primer set represented by SEQ ID NO: 600, the first allele-specific forward primer represented by SEQ ID NO: 601 for SK08_120, the second allele represented by SEQ ID NO: 602 A trait-specific forward primer and a set of reverse primers represented by SEQ ID NO: 603, a first allele-specific forward primer represented by SEQ ID NO: 604 for SK08_137, a second allele-specific forward primer represented by SEQ ID NO: 605, and SEQ ID NO: 606 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 607 for SK08_142, a second allele-specific forward primer represented by SEQ ID NO: 608, and a reverse primer set represented by SEQ ID NO: 609, A set of a first allele-specific forward primer represented by SEQ ID NO: 610 for SK08_148, a second allele-specific forward primer represented by SEQ ID NO: 611 and a reverse primer set represented by SEQ ID NO: 612, SEQ ID NO: 613 for SK08_156 A set of first allele-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 614 and a reverse primer set represented by SEQ ID NO: 615, the first allele-specific forward primer represented by SEQ ID NO: 616 for SK08_164 , the second allele-specific forward primer represented by SEQ ID NO: 617 and the reverse primer set represented by SEQ ID NO: 618, the first allele-specific forward primer represented by SEQ ID NO: 619 for SK08_172, the first allele-specific forward primer represented by SEQ ID NO: 620 2 allele-specific forward primers and reverse primer set represented by SEQ ID NO: 621, the first allele-specific forward primer represented by SEQ ID NO: 622 for SK08_179, the second allele-specific forward primer represented by SEQ ID NO: 623 and sequence Reverse primer set represented by No. 624, the first allele-specific forward primer represented by SEQ ID NO: 625 for SK08_187, the second allele-specific forward primer represented by SEQ ID NO: 626, and the reverse primer represented by SEQ ID NO: 627 Set, first allele specific forward primer represented by SEQ ID NO: 628 for SK08_197, second allele specific forward primer represented by SEQ ID NO: 629 and reverse primer set represented by SEQ ID NO: 630, SEQ ID NO: 631 for SK08_203 The first allele-specific forward primer represented by SEQ ID NO: 632, the second allele-specific forward primer represented by SEQ ID NO: 633, and the reverse primer set represented by SEQ ID NO: 633, the first allele-specific represented by SEQ ID NO: 634 for SK08_209 Forward primer, a second allele-specific forward primer represented by SEQ ID NO: 635 and a set of reverse primers represented by SEQ ID NO: 636, a first allele-specific forward primer represented by SEQ ID NO: 637 for SK08_216, SEQ ID NO: 638 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 639, a first allele-specific forward primer represented by SEQ ID NO: 640 for SK08_219, and a second allele-specific forward primer represented by SEQ ID NO: 641 and a reverse primer set represented by SEQ ID NO: 642, a first allele-specific forward primer represented by SEQ ID NO: 643 for SK08_227, a second allele-specific forward primer represented by SEQ ID NO: 644, and a reverse sequence represented by SEQ ID NO: 645. The anti-primer set, the first allele-specific forward primer represented by SEQ ID NO: 646 for SK08_233, the second allele-specific forward primer represented by SEQ ID NO: 647, and the reverse primer set represented by SEQ ID NO: 648, the sequence for SK08_241 A first allele-specific forward primer represented by SEQ ID NO: 649, a second allele-specific forward primer represented by SEQ ID NO: 650, and a reverse primer set represented by SEQ ID NO: 651, the first allele represented by SEQ ID NO: 652 for SK08_254 A set of trait-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 653 and a reverse primer set represented by SEQ ID NO: 654, a first allele-specific forward primer represented by SEQ ID NO: 655 for SK08_260, SEQ ID NO: 656 A second allele-specific forward primer represented by and a reverse primer set represented by SEQ ID NO: 657, a first allele-specific forward primer represented by SEQ ID NO: 658 for SK09_004, and a second allele-specific represented by SEQ ID NO: 659 A forward primer and a reverse primer set represented by SEQ ID NO: 660, a first allele-specific forward primer represented by SEQ ID NO: 661 for SK09_028, a second allele-specific forward primer represented by SEQ ID NO: 662, and SEQ ID NO: 663 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 664 for SK09_035, a second allele-specific forward primer represented by SEQ ID NO: 665, and a reverse primer set represented by SEQ ID NO: 666, to SK09_042 The first allele-specific forward primer represented by SEQ ID NO: 667, the second allele-specific forward primer represented by SEQ ID NO: 668, and the reverse primer set represented by SEQ ID NO: 669, the first allele-specific forward primer represented by SEQ ID NO: 670 for SK09_052 1 allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 671 and the reverse primer set represented by SEQ ID NO: 672, the first allele-specific forward primer represented by SEQ ID NO: 673 for SK09_056, sequence The second allele-specific forward primer represented by SEQ ID NO: 674 and the reverse primer set represented by SEQ ID NO: 675, the first allele-specific forward primer represented by SEQ ID NO: 676 for SK09_062, the second allele represented by SEQ ID NO: 677 A trait-specific forward primer and a reverse primer set represented by SEQ ID NO: 678, a first allele-specific forward primer represented by SEQ ID NO: 679 for SK09_068, a second allele-specific forward primer represented by SEQ ID NO: 680, and SEQ ID NO: 681 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 682 for SK09_075, a second allele-specific forward primer represented by SEQ ID NO: 683, and a reverse primer set represented by SEQ ID NO: 684, A set of a first allele-specific forward primer represented by SEQ ID NO: 685 for SK09_093, a second allele-specific forward primer represented by SEQ ID NO: 686 and a reverse primer set represented by SEQ ID NO: 687, SEQ ID NO: 688 for SK09_123 A set of first allele-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 689 and a reverse primer set represented by SEQ ID NO: 690, the first allele-specific forward primer represented by SEQ ID NO: 691 for SK09_132 , the second allele-specific forward primer represented by SEQ ID NO: 692 and the reverse primer set represented by SEQ ID NO: 693, the first allele-specific forward primer represented by SEQ ID NO: 694 for SK09_140, the first allele-specific forward primer represented by SEQ ID NO: 695 2 allele-specific forward primers and reverse primer set represented by SEQ ID NO: 696, the first allele-specific forward primer represented by SEQ ID NO: 697 for SK09_154, the second allele-specific forward primer represented by SEQ ID NO: 698 and sequence Reverse primer set represented by No. 699, the first allele-specific forward primer represented by SEQ ID NO: 700 for SK09_162, the second allele-specific forward primer represented by SEQ ID NO: 701, and the reverse primer represented by SEQ ID NO: 702 Set, a first allele specific forward primer represented by SEQ ID NO: 703 for SK09_160, a second allele specific forward primer represented by SEQ ID NO: 704 and a reverse primer set represented by SEQ ID NO: 705, SEQ ID NO: 706 for SK09_169 The first allele-specific forward primer represented by SEQ ID NO: 707, the second allele-specific forward primer represented by SEQ ID NO: 708, and the reverse primer set represented by SEQ ID NO: 708, the first allele-specific represented by SEQ ID NO: 709 for SK09_173 Forward primer, second allele-specific forward primer represented by SEQ ID NO: 710 and reverse primer set represented by SEQ ID NO: 711, first allele-specific forward primer represented by SEQ ID NO: 712 for SK09_177, SEQ ID NO: 713 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 714, a first allele-specific forward primer represented by SEQ ID NO: 715 for SK09_181, and a second allele-specific forward primer represented by SEQ ID NO: 716 and a reverse primer set represented by SEQ ID NO: 717, a first allele-specific forward primer represented by SEQ ID NO: 718 for SK09_186, a second allele-specific forward primer represented by SEQ ID NO: 719, and a reverse sequence represented by SEQ ID NO: 720. The anti-primer set, the first allele-specific forward primer represented by SEQ ID NO: 721 for SK09_189, the second allele-specific forward primer represented by SEQ ID NO: 722, and the reverse primer set represented by SEQ ID NO: 723, the sequence for SK09_197 The first allele-specific forward primer represented by SEQ ID NO: 724, the second allele-specific forward primer represented by SEQ ID NO: 725, and the reverse primer set represented by SEQ ID NO: 726, the first allele represented by SEQ ID NO: 727 for SK09_208 Trait specific forward primer, second allele specific forward primer represented by SEQ ID NO: 728 and reverse primer set represented by SEQ ID NO: 729, first allele specific forward primer represented by SEQ ID NO: 730 for SK09_213, SEQ ID NO: 731 A second allele-specific forward primer represented by and a reverse primer set represented by SEQ ID NO: 732, a first allele-specific forward primer represented by SEQ ID NO: 733 for SK09_224, and a second allele-specific represented by SEQ ID NO: 734 A forward primer and a reverse primer set represented by SEQ ID NO: 735, a first allele-specific forward primer represented by SEQ ID NO: 736 for SK09_228, a second allele-specific forward primer represented by SEQ ID NO: 737, and SEQ ID NO: 738 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 739 for SK10_005, a second allele-specific forward primer represented by SEQ ID NO: 740, and a reverse primer set represented by SEQ ID NO: 741, SK10_011 The first allele-specific forward primer represented by SEQ ID NO: 742, the second allele-specific forward primer represented by SEQ ID NO: 743, and the reverse primer set represented by SEQ ID NO: 744, the first allele-specific forward primer represented by SEQ ID NO: 745 for SK10_014 1 allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 746 and the reverse primer set represented by SEQ ID NO: 747, the first allele-specific forward primer represented by SEQ ID NO: 748 for SK10_019, sequence The second allele-specific forward primer represented by No. 749 and the reverse primer set represented by SEQ ID NO: 750, the first allele-specific forward primer represented by SEQ ID NO: 751 for SK10_028, the second allele represented by SEQ ID NO: 752 A trait-specific forward primer and a reverse primer set represented by SEQ ID NO: 753, a first allele-specific forward primer represented by SEQ ID NO: 754 for SK10_032, a second allele-specific forward primer represented by SEQ ID NO: 755, and SEQ ID NO: 756 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 757 for SK10_038, a second allele-specific forward primer represented by SEQ ID NO: 758, and a reverse primer set represented by SEQ ID NO: 759, A first allele-specific forward primer represented by SEQ ID NO: 760 for SK10_046, a second allele-specific forward primer represented by SEQ ID NO: 761 and a reverse primer set represented by SEQ ID NO: 762, SEQ ID NO: 763 for SK10_053 A set of first allele-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 764 and a reverse primer set represented by SEQ ID NO: 765, the first allele-specific forward primer represented by SEQ ID NO: 766 for SK10_061 , the second allele-specific forward primer represented by SEQ ID NO: 757 and the reverse primer set represented by SEQ ID NO: 768, the first allele-specific forward primer represented by SEQ ID NO: 769 for SK10_076, the first allele-specific forward primer represented by SEQ ID NO: 770 2 allele-specific forward primers and reverse primer set represented by SEQ ID NO: 771, the first allele-specific forward primer represented by SEQ ID NO: 772 for SK10_086, the second allele-specific forward primer represented by SEQ ID NO: 773 and sequence Reverse primer set represented by No. 774, the first allele-specific forward primer represented by SEQ ID NO: 775 for SK10_092, the second allele-specific forward primer represented by SEQ ID NO: 776, and the reverse primer represented by SEQ ID NO: 777 Set, a first allele specific forward primer represented by SEQ ID NO: 778 for SK10_097, a second allele specific forward primer represented by SEQ ID NO: 779 and a reverse primer set represented by SEQ ID NO: 780, SEQ ID NO: 781 for SK10_113 The first allele-specific forward primer represented by SEQ ID NO: 782, the second allele-specific forward primer represented by SEQ ID NO: 783, and the reverse primer set represented by SEQ ID NO: 783, the first allele-specific represented by SEQ ID NO: 784 for SK10_123 Forward primer, a second allele-specific forward primer represented by SEQ ID NO: 785 and a set of reverse primers represented by SEQ ID NO: 786, a first allele-specific forward primer represented by SEQ ID NO: 787 for SK10_128, SEQ ID NO: 788 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 789, a first allele-specific forward primer represented by SEQ ID NO: 790 for SK10_131, and a second allele-specific forward primer represented by SEQ ID NO: 791 and a reverse primer set represented by SEQ ID NO: 792, a first allele-specific forward primer represented by SEQ ID NO: 793 for SK10_135, a second allele-specific forward primer represented by SEQ ID NO: 794, and a reverse sequence represented by SEQ ID NO: 795. The anti-primer set, the first allele-specific forward primer represented by SEQ ID NO: 796 for SK10_146, the second allele-specific forward primer represented by SEQ ID NO: 797, and the reverse primer set represented by SEQ ID NO: 798, the sequence for SK10_153 The first allele-specific forward primer represented by SEQ ID NO: 799, the second allele-specific forward primer represented by SEQ ID NO: 800, and the reverse primer set represented by SEQ ID NO: 801, the first allele represented by SEQ ID NO: 802 for SK10_161 Trait specific forward primer, second allele specific forward primer represented by SEQ ID NO: 803 and reverse primer set represented by SEQ ID NO: 804, first allele specific forward primer represented by SEQ ID NO: 805 for SK10_167, SEQ ID NO: 806 A second allele-specific forward primer represented by and a reverse primer set represented by SEQ ID NO: 807, a first allele-specific forward primer represented by SEQ ID NO: 808 for SK10_174, and a second allele-specific represented by SEQ ID NO: 809 A forward primer and a reverse primer set represented by SEQ ID NO: 810, a first allele-specific forward primer represented by SEQ ID NO: 811 for SK10_182, a second allele-specific forward primer represented by SEQ ID NO: 812, and SEQ ID NO: 813 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 814 for SK11_001, a second allele-specific forward primer represented by SEQ ID NO: 815, and a reverse primer set represented by SEQ ID NO: 816, SK11_007 The first allele-specific forward primer represented by SEQ ID NO: 817, the second allele-specific forward primer represented by SEQ ID NO: 818, and the reverse primer set represented by SEQ ID NO: 819, the first allele-specific forward primer represented by SEQ ID NO: 820 for SK11_015 1 allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 821 and the reverse primer set represented by SEQ ID NO: 822, the first allele-specific forward primer represented by SEQ ID NO: 823 for SK11_022, sequence The second allele-specific forward primer represented by SEQ ID NO: 824 and the reverse primer set represented by SEQ ID NO: 825, the first allele-specific forward primer represented by SEQ ID NO: 826 for SK11_027, the second allele represented by SEQ ID NO: 827 A trait-specific forward primer and a set of reverse primers represented by SEQ ID NO: 828, a first allele-specific forward primer represented by SEQ ID NO: 829 for SK11_035, a second allele-specific forward primer represented by SEQ ID NO: 830, and SEQ ID NO: 831 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 832 for SK11_042, a second allele-specific forward primer represented by SEQ ID NO: 833, and a reverse primer set represented by SEQ ID NO: 834, A set of a first allele-specific forward primer represented by SEQ ID NO: 835 for SK11_050, a second allele-specific forward primer represented by SEQ ID NO: 836 and a reverse primer set represented by SEQ ID NO: 837, SEQ ID NO: 838 for SK11_059 A set of first allele-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 839 and a reverse primer set represented by SEQ ID NO: 840, the first allele-specific forward primer represented by SEQ ID NO: 841 for SK11_066 , the second allele-specific forward primer represented by SEQ ID NO: 842 and the reverse primer set represented by SEQ ID NO: 843, the first allele-specific forward primer represented by SEQ ID NO: 844 for SK11_073, the first allele-specific forward primer represented by SEQ ID NO: 845 2 allele-specific forward primers and reverse primer set represented by SEQ ID NO: 846, the first allele-specific forward primer represented by SEQ ID NO: 847 for SK11_084, the second allele-specific forward primer represented by SEQ ID NO: 848 and sequence Reverse primer set represented by No. 849, the first allele-specific forward primer represented by SEQ ID NO: 850 for SK11_092, the second allele-specific forward primer represented by SEQ ID NO: 851, and the reverse primer represented by SEQ ID NO: 852 Set, a first allele specific forward primer represented by SEQ ID NO: 853 for SK11_102, a second allele specific forward primer represented by SEQ ID NO: 854 and a reverse primer set represented by SEQ ID NO: 855, SEQ ID NO: 856 for SK11_109 The first allele-specific forward primer represented by SEQ ID NO: 857, the second allele-specific forward primer represented by SEQ ID NO: 858, and the reverse primer set represented by SEQ ID NO: 858, the first allele-specific represented by SEQ ID NO: 859 for SK11_117 Forward primer, a second allele-specific forward primer represented by SEQ ID NO: 860 and a set of reverse primers represented by SEQ ID NO: 861, a first allele-specific forward primer represented by SEQ ID NO: 862 for SK11_125, SEQ ID NO: 863 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 864, a first allele-specific forward primer represented by SEQ ID NO: 865 for SK11_130, and a second allele-specific forward primer represented by SEQ ID NO: 866 and a set of reverse primers represented by SEQ ID NO: 867, a first allele-specific forward primer represented by SEQ ID NO: 868 for SK11_137, a second allele-specific forward primer represented by SEQ ID NO: 869, and a reverse sequence represented by SEQ ID NO: 870. The anti-primer set, the first allele-specific forward primer represented by SEQ ID NO: 871 for SK12_007, the second allele-specific forward primer represented by SEQ ID NO: 872, and the reverse primer set represented by SEQ ID NO: 873, the sequence for SK12_019 A set of first allele-specific forward primers represented by SEQ ID NO: 874, second allele-specific forward primers represented by SEQ ID NO: 875 and reverse primers represented by SEQ ID NO: 876, the first allele represented by SEQ ID NO: 877 for SK12_022 A set of trait-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 878 and a set of reverse primers represented by SEQ ID NO: 879, a first allele-specific forward primer represented by SEQ ID NO: 880 for SK12_033, SEQ ID NO: 881 A second allele-specific forward primer represented by and a reverse primer set represented by SEQ ID NO: 882, a first allele-specific forward primer represented by SEQ ID NO: 883 for SK12_042, and a second allele-specific represented by SEQ ID NO: 884 A forward primer and a reverse primer set represented by SEQ ID NO: 885, a first allele-specific forward primer represented by SEQ ID NO: 886 for SK12_052, a second allele-specific forward primer represented by SEQ ID NO: 887, and SEQ ID NO: 888 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 889 for SK12_066, a second allele-specific forward primer represented by SEQ ID NO: 890, and a reverse primer set represented by SEQ ID NO: 891, SK12_073 The first allele-specific forward primer represented by SEQ ID NO: 892, the second allele-specific forward primer represented by SEQ ID NO: 893, and the reverse primer set represented by SEQ ID NO: 894, the first allele-specific forward primer represented by SEQ ID NO: 895 for SK12_080 1 allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 896 and the reverse primer set represented by SEQ ID NO: 897, the first allele-specific forward primer represented by SEQ ID NO: 898 for SK12_089, sequence The second allele-specific forward primer represented by No. 899 and the reverse primer set represented by SEQ ID NO: 900, the first allele-specific forward primer represented by SEQ ID NO: 901 for SK12_096, the second allele represented by SEQ ID NO: 902 A trait-specific forward primer and a reverse primer set represented by SEQ ID NO: 903, a first allele-specific forward primer represented by SEQ ID NO: 904 for SK12_110, a second allele-specific forward primer represented by SEQ ID NO: 905, and SEQ ID NO: 906 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 907 for SK12_118, a second allele-specific forward primer represented by SEQ ID NO: 908, and a reverse primer set represented by SEQ ID NO: 909, A first allele-specific forward primer represented by SEQ ID NO: 910 for SK12_123, a second allele-specific forward primer represented by SEQ ID NO: 911 and a reverse primer set represented by SEQ ID NO: 912, SEQ ID NO: 913 for SK12_128 A set of first allele-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 914 and a reverse primer set represented by SEQ ID NO: 915, the first allele-specific forward primer represented by SEQ ID NO: 916 for SK12_134 , the second allele-specific forward primer represented by SEQ ID NO: 917 and the reverse primer set represented by SEQ ID NO: 918, the first allele-specific forward primer represented by SEQ ID NO: 919 for SK12_138, the first allele-specific forward primer represented by SEQ ID NO: 920 2 allele-specific forward primers and reverse primer set represented by SEQ ID NO: 921, the first allele-specific forward primer represented by SEQ ID NO: 922 for SK12_147, the second allele-specific forward primer represented by SEQ ID NO: 923, and the sequence Reverse primer set represented by No. 924, the first allele-specific forward primer represented by SEQ ID NO: 925 for SK12_151, the second allele-specific forward primer represented by SEQ ID NO: 926, and the reverse primer represented by SEQ ID NO: 927 Set, first allele specific forward primer represented by SEQ ID NO: 928 to SK12_161, second allele specific forward primer represented by SEQ ID NO: 929 and reverse primer set represented by SEQ ID NO: 930, SEQ ID NO: 931 to SK13_003 The first allele-specific forward primer represented by SEQ ID NO: 932, the second allele-specific forward primer represented by SEQ ID NO: 933, and the reverse primer set represented by SEQ ID NO: 933, the first allele-specific represented by SEQ ID NO: 934 for SK13_008 Forward primer, a second allele-specific forward primer represented by SEQ ID NO: 935 and a set of reverse primers represented by SEQ ID NO: 936, a first allele-specific forward primer represented by SEQ ID NO: 937 for SK13_015, SEQ ID NO: 938 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 939, a first allele-specific forward primer represented by SEQ ID NO: 940 for SK13_023, and a second allele-specific forward primer represented by SEQ ID NO: 941 and a reverse primer set represented by SEQ ID NO: 942, a first allele-specific forward primer represented by SEQ ID NO: 943 for SK13_055, a second allele-specific forward primer represented by SEQ ID NO: 944, and a reverse sequence represented by SEQ ID NO: 945. The anti-primer set, the first allele-specific forward primer represented by SEQ ID NO: 946 for SK13_061, the second allele-specific forward primer represented by SEQ ID NO: 947, and the reverse primer set represented by SEQ ID NO: 948, the sequence for SK13_067 The first allele-specific forward primer represented by SEQ ID NO: 949, the second allele-specific forward primer represented by SEQ ID NO: 950, and the reverse primer set represented by SEQ ID NO: 951, the first allele represented by SEQ ID NO: 952 for SK13_071 Trait specific forward primer, second allele specific forward primer represented by SEQ ID NO: 953 and reverse primer set represented by SEQ ID NO: 954, first allele specific forward primer represented by SEQ ID NO: 955 for SK13_080, SEQ ID NO: 956 The second allele-specific forward primer represented by and the reverse primer set represented by SEQ ID NO: 957, the first allele-specific forward primer represented by SEQ ID NO: 958 for SK13_088, the second allele-specific represented by SEQ ID NO: 959 A forward primer and a reverse primer set represented by SEQ ID NO: 960, a first allele-specific forward primer represented by SEQ ID NO: 961 for SK13_094, a second allele-specific forward primer represented by SEQ ID NO: 962, and SEQ ID NO: 963 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 964 for SK13_103, a second allele-specific forward primer represented by SEQ ID NO: 965, and a reverse primer set represented by SEQ ID NO: 966, SK13_107 A set of the first allele-specific forward primer represented by SEQ ID NO: 967, the second allele-specific forward primer represented by SEQ ID NO: 968, and the reverse primer set represented by SEQ ID NO: 969, the first allele-specific forward primer represented by SEQ ID NO: 970 for SK13_113 1 allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 971 and the reverse primer set represented by SEQ ID NO: 972, the first allele-specific forward primer represented by SEQ ID NO: 973 for SK13_123, sequence The second allele-specific forward primer represented by No. 974 and the reverse primer set represented by SEQ ID NO: 975, the first allele-specific forward primer represented by SEQ ID NO: 976 for SK13_127, the second allele represented by SEQ ID NO: 977 A trait-specific forward primer and a set of reverse primers represented by SEQ ID NO: 978, a first allele-specific forward primer represented by SEQ ID NO: 979 for SK13_131, a second allele-specific forward primer represented by SEQ ID NO: 980, and SEQ ID NO: 981 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 982 for SK13_138, a second allele-specific forward primer represented by SEQ ID NO: 983, and a reverse primer set represented by SEQ ID NO: 984, A first allele-specific forward primer represented by SEQ ID NO: 985 for SK13_150, a second allele-specific forward primer represented by SEQ ID NO: 986 and a reverse primer set represented by SEQ ID NO: 987, SEQ ID NO: 988 for SK13_157 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 989, and the reverse primer set represented by SEQ ID NO: 990, the first allele-specific forward primer represented by SEQ ID NO: 991 for SK13_164 , Second allele-specific forward primers represented by SEQ ID NO: 992 and 19 or more primer sets selected from the group consisting of a reverse primer set represented by SEQ ID NO: 993, KASP primers for sesame gene association map creation set.
A kit for creating a genetic map of sesame varieties, comprising the primer set of claim 1 and reagents for performing an amplification reaction.
The first allele-specific forward primer represented by SEQ ID NO: 1 for SK01_007, the second allele-specific forward primer represented by SEQ ID NO: 2, and the reverse primer set represented by SEQ ID NO: 3, SEQ ID NO: 4 for SK01_012 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 5 and the reverse primer set represented by SEQ ID NO: 6, the first allele-specific forward primer represented by SEQ ID NO: 7 for SK01_018, sequence The second allele-specific forward primer represented by No. 8 and the reverse primer set represented by SEQ ID NO: 9, the first allele-specific forward primer represented by SEQ ID NO: 10 for SK01_025, the second allele represented by SEQ ID NO: 11 A set of specific forward primers and reverse primers represented by SEQ ID NO: 12, a first allele-specific forward primer represented by SEQ ID NO: 13 for SK01_030, a second allele-specific forward primer represented by SEQ ID NO: 14, and SEQ ID NO: 15 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 16 for SK01_038, a second allele-specific forward primer represented by SEQ ID NO: 17, and a reverse primer set represented by SEQ ID NO: 18, sequence for SK01_046 The first allele-specific forward primer represented by SEQ ID NO: 19, the second allele-specific forward primer represented by SEQ ID NO: 20, and the reverse primer set represented by SEQ ID NO: 21, the first allele represented by SEQ ID NO: 22 for SK01_054 A specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 23 and a reverse primer set represented by SEQ ID NO: 24, a first allele-specific forward primer represented by SEQ ID NO: 25 for SKJ01_070, represented by SEQ ID NO: 26 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 27, a first allele-specific forward primer represented by SEQ ID NO: 28 for SK01_084, a second allele-specific forward primer represented by SEQ ID NO: 29, and A reverse primer set represented by SEQ ID NO: 30, a first allele-specific forward primer represented by SEQ ID NO: 31 for SK01_093, a second allele-specific forward primer represented by SEQ ID NO: 32, and a reverse primer set represented by SEQ ID NO: 33 , the first allele-specific forward primer represented by SEQ ID NO: 34 for SK01_102, the second allele-specific forward primer represented by SEQ ID NO: 35 and the reverse primer set represented by SEQ ID NO: 36, SEQ ID NO: 37 for SK01_109 A set of first allele-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 38 and a reverse primer set represented by SEQ ID NO: 39, the first allele-specific forward primer represented by SEQ ID NO: 40 for SK01_113 , the second allele-specific forward primer represented by SEQ ID NO: 41 and the reverse primer set represented by SEQ ID NO: 42, the first allele-specific forward primer represented by SEQ ID NO: 43 for SK01_120, the second allele-specific forward primer represented by SEQ ID NO: 44 Allele-specific forward primer and reverse primer set represented by SEQ ID NO: 45, first allele-specific forward primer represented by SEQ ID NO: 46 for SK01_128, second allele-specific forward primer represented by SEQ ID NO: 47 and SEQ ID NO: 48 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 49 for SK01_133, a second allele-specific forward primer represented by SEQ ID NO: 50, and a reverse primer set represented by SEQ ID NO: 51, to SK01_139 A set of a first allele-specific forward primer represented by SEQ ID NO: 52, a second allele-specific forward primer represented by SEQ ID NO: 53, and a reverse primer set represented by SEQ ID NO: 54, a first set represented by SEQ ID NO: 55 for SK01_144 An allele-specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 56 and a set of reverse primers represented by SEQ ID NO: 57, a first allele-specific forward primer represented by SEQ ID NO: 58 for SK01_152, SEQ ID NO: 59 A second allele-specific forward primer represented by and a reverse primer set represented by SEQ ID NO: 60, a first allele-specific forward primer represented by SEQ ID NO: 61 for SK01_160, and a second allele-specific forward primer represented by SEQ ID NO: 62 A set of primers and reverse primers represented by SEQ ID NO: 63, a first allele-specific forward primer represented by SEQ ID NO: 64 for SK01_174, a second allele-specific forward primer represented by SEQ ID NO: 65, and a reverse primer represented by SEQ ID NO: 66 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 67 for SK01_181, a second allele-specific forward primer represented by SEQ ID NO: 68, and a reverse primer set represented by SEQ ID NO: 69, SEQ ID NO: 70 for SK01_187 The first allele-specific forward primer represented by SEQ ID NO: 71, the second allele-specific forward primer represented by SEQ ID NO: 72, and the reverse primer set represented by SEQ ID NO: 72, the first allele-specific forward primer represented by SEQ ID NO: 73 for SK01_194 Primers, the second allele-specific forward primer represented by SEQ ID NO: 74 and the reverse primer set represented by SEQ ID NO: 75, the first allele-specific forward primer represented by SEQ ID NO: 76 for SK01_201, the first allele-specific forward primer represented by SEQ ID NO: 77 2 Allele-specific forward primer and reverse primer set represented by SEQ ID NO: 78, the first allele-specific forward primer represented by SEQ ID NO: 79 for SK02_007, the second allele-specific forward primer represented by SEQ ID NO: 80 and SEQ ID NO: A reverse primer set represented by SEQ ID NO: 81, a first allele-specific forward primer represented by SEQ ID NO: 82 for SK02_013, a second allele-specific forward primer represented by SEQ ID NO: 83, and a reverse primer set represented by SEQ ID NO: 84, SK02_022 The first allele-specific forward primer represented by SEQ ID NO: 85, the second allele-specific forward primer represented by SEQ ID NO: 86, and the reverse primer set represented by SEQ ID NO: 87, SEQ ID NO: 88 for SK02_027 The first allele specific forward primer, the second allele specific forward primer represented by SEQ ID NO: 89 and the reverse primer set represented by SEQ ID NO: 90, the first allele specific forward primer represented by SEQ ID NO: 91 for SK02-036 Primers, the second allele-specific forward primer represented by SEQ ID NO: 92 and the reverse primer set represented by SEQ ID NO: 93, the first allele-specific forward primer represented by SEQ ID NO: 94 for SK02_041, SEQ ID NO: 95 A second allele-specific forward primer and a set of reverse primers represented by SEQ ID NO: 96, a first allele-specific forward primer represented by SEQ ID NO: 97 for SK02_050, a second allele-specific forward primer represented by SEQ ID NO: 98, and A reverse primer set represented by SEQ ID NO: 99, a first allele-specific forward primer represented by SEQ ID NO: 100 for SK02_059, a second allele-specific forward primer represented by SEQ ID NO: 101, and a reverse primer represented by SEQ ID NO: 102 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 103 for SK02_070, a second allele-specific forward primer represented by SEQ ID NO: 104, and a reverse primer set represented by SEQ ID NO: 105, SEQ ID NO: SK02_076 The first allele-specific forward primer represented by SEQ ID NO: 106, the second allele-specific forward primer represented by SEQ ID NO: 107, and the reverse primer set represented by SEQ ID NO: 108, the first allele represented by SEQ ID NO: 109 for SK02_081 A specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 110 and a set of reverse primers represented by SEQ ID NO: 111, a first allele-specific forward primer represented by SEQ ID NO: 112 for SK02_087, SEQ ID NO: 113 The second allele-specific forward primer and the reverse primer set represented by SEQ ID NO: 114, the first allele-specific forward primer represented by SEQ ID NO: 115 for SK02_089, and the second allele-specific forward primer represented by SEQ ID NO: 116 Primers and a reverse primer set represented by SEQ ID NO: 117, a first allele-specific forward primer represented by SEQ ID NO: 118 for SK02_097, a second allele-specific forward primer represented by SEQ ID NO: 119, and SEQ ID NO: 120 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 121 for SK02_103, a second allele-specific forward primer represented by SEQ ID NO: 122, and a reverse primer set represented by SEQ ID NO: 123, for SK02_110 A set of first allele-specific forward primers represented by SEQ ID NO: 124, second allele-specific forward primers represented by SEQ ID NO: 125 and reverse primers represented by SEQ ID NO: 126, the first set represented by SEQ ID NO: 127 for SK02_117 Allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 128 and the reverse primer set represented by SEQ ID NO: 129, the first allele-specific forward primer represented by SEQ ID NO: 130 for SK02_121, SEQ ID NO: The second allele-specific forward primer represented by SEQ ID NO: 131 and the reverse primer set represented by SEQ ID NO: 132, the first allele-specific forward primer represented by SEQ ID NO: 133 for SK02_133, the second allele represented by SEQ ID NO: 134 A specific forward primer and a set of reverse primers represented by SEQ ID NO: 135, a first allele-specific forward primer represented by SEQ ID NO: 136 for SK02_140, a second allele-specific forward primer represented by SEQ ID NO: 137, and SEQ ID NO: 138 The reverse primer set represented by, the first allele-specific forward primer represented by SEQ ID NO: 139 for SK02_151, the second allele-specific forward primer represented by SEQ ID NO: 140, and the reverse primer set represented by SEQ ID NO: 141, SK02_160 The first allele-specific forward primer represented by SEQ ID NO: 142, the second allele-specific forward primer represented by SEQ ID NO: 143, and the reverse primer set represented by SEQ ID NO: 144, SEQ ID NO: 145 for SK02_167 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 146 and the reverse primer set represented by SEQ ID NO: 147, the first allele-specific forward primer represented by SEQ ID NO: 148 for SK02_179, The second allele-specific forward primer represented by SEQ ID NO: 149 and the reverse primer set represented by SEQ ID NO: 150, the first allele-specific forward primer represented by SEQ ID NO: 151 for SK03_003, and the second allele-specific forward primer represented by SEQ ID NO: 152 Allele-specific forward primer and reverse primer set represented by SEQ ID NO: 153, the first allele-specific forward primer represented by SEQ ID NO: 154 for SK03_012, the second allele-specific forward primer represented by SEQ ID NO: 155, and SEQ ID NO: A reverse primer set represented by SEQ ID NO: 156, a first allele-specific forward primer represented by SEQ ID NO: 157 for SK03_020, a second allele-specific forward primer represented by SEQ ID NO: 158, and a reverse primer set represented by SEQ ID NO: 159 , the first allele-specific forward primer represented by SEQ ID NO: 160 for SK03_029, the second allele-specific forward primer represented by SEQ ID NO: 161 and the reverse primer set represented by SEQ ID NO: 162, SEQ ID NO: 163 for SK03_030. The first allele-specific forward primer represented by SEQ ID NO: 164, the second allele-specific forward primer represented by SEQ ID NO: 165, and the reverse primer set represented by SEQ ID NO: 165, the first allele-specific forward primer represented by SEQ ID NO: 166 for SK03_038 Primers, the second allele-specific forward primer represented by SEQ ID NO: 167 and the reverse primer set represented by SEQ ID NO: 168, the first allele-specific forward primer represented by SEQ ID NO: 169 for SK03_044, SEQ ID NO: 170 A second allele-specific forward primer and a set of reverse primers represented by SEQ ID NO: 171, a first allele-specific forward primer represented by SEQ ID NO: 172 for SK03_050, a second allele-specific forward primer represented by SEQ ID NO: 173, and A reverse primer set represented by SEQ ID NO: 174, a first allele-specific forward primer represented by SEQ ID NO: 175 for SK03_055, a second allele-specific forward primer represented by SEQ ID NO: 176, and a reverse primer represented by SEQ ID NO: 177 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 178 for SK03_063, a second allele-specific forward primer represented by SEQ ID NO: 179, and a reverse primer set represented by SEQ ID NO: 180, SEQ ID NO: SK03_070 The first allele-specific forward primer represented by SEQ ID NO: 181, the second allele-specific forward primer represented by SEQ ID NO: 182, and the reverse primer set represented by SEQ ID NO: 183, the first allele represented by SEQ ID NO: 184 for SK03_079 A specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 185 and a set of reverse primers represented by SEQ ID NO: 186, a first allele-specific forward primer represented by SEQ ID NO: 187 for SK03_094, SEQ ID NO: 188 The second allele-specific forward primer and the reverse primer set represented by SEQ ID NO: 189, the first allele-specific forward primer represented by SEQ ID NO: 190 for SK03_098, and the second allele-specific forward primer represented by SEQ ID NO: 191 Primers and a set of reverse primers represented by SEQ ID NO: 192, a first allele-specific forward primer represented by SEQ ID NO: 193 for SK03_102, a second allele-specific forward primer represented by SEQ ID NO: 194, and SEQ ID NO: 195 The reverse primer set, the first allele-specific forward primer represented by SEQ ID NO: 196 for SK03_108, the second allele-specific forward primer represented by SEQ ID NO: 197, and the reverse primer set represented by SEQ ID NO: 198, for SK03_116 A set of first allele-specific forward primers represented by SEQ ID NO: 199, second allele-specific forward primers represented by SEQ ID NO: 200 and reverse primers represented by SEQ ID NO: 201, the first set represented by SEQ ID NO: 202 for SK03_120 Allele-specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 203 and a set of reverse primers represented by SEQ ID NO: 204, a first allele-specific forward primer represented by SEQ ID NO: 205 for SK03_126, SEQ ID NO: The second allele-specific forward primer represented by SEQ ID NO: 206 and the reverse primer set represented by SEQ ID NO: 207, the first allele-specific forward primer represented by SEQ ID NO: 208 for SK03_141, the second allele represented by SEQ ID NO: 209 A specific forward primer and a set of reverse primers represented by SEQ ID NO: 210, a first allele-specific forward primer represented by SEQ ID NO: 211 for SK03_147, a second allele-specific forward primer represented by SEQ ID NO: 212, and SEQ ID NO: 213 The reverse primer set represented by, the first allele-specific forward primer represented by SEQ ID NO: 214 for SK03_151, the second allele-specific forward primer represented by SEQ ID NO: 215, and the reverse primer set represented by SEQ ID NO: 216, SK03_158 The first allele-specific forward primer represented by SEQ ID NO: 217, the second allele-specific forward primer represented by SEQ ID NO: 218, and the reverse primer set represented by SEQ ID NO: 219, SEQ ID NO: 220 for SK03_166 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 221 and the reverse primer set represented by SEQ ID NO: 222, the first allele-specific forward primer represented by SEQ ID NO: 223 for SK03_173, The second allele-specific forward primer represented by SEQ ID NO: 224 and the reverse primer set represented by SEQ ID NO: 225, the first allele-specific forward primer represented by SEQ ID NO: 226 for SK03_180, the second allele-specific forward primer represented by SEQ ID NO: 227 Allele-specific forward primer and reverse primer set represented by SEQ ID NO: 228, the first allele-specific forward primer represented by SEQ ID NO: 229 for SK03_191, the second allele-specific forward primer represented by SEQ ID NO: 230, and SEQ ID NO: A reverse primer set represented by SEQ ID NO: 231, a first allele-specific forward primer represented by SEQ ID NO: 232 for SK03_203, a second allele-specific forward primer represented by SEQ ID NO: 233, and a reverse primer set represented by SEQ ID NO: 234 , the first allele-specific forward primer represented by SEQ ID NO: 235 for SK03_219, the second allele-specific forward primer represented by SEQ ID NO: 236 and the reverse primer set represented by SEQ ID NO: 237, SEQ ID NO: 238 for SK03_226 The first allele-specific forward primer represented by SEQ ID NO: 239, the second allele-specific forward primer represented by SEQ ID NO: 240, and the set of reverse primers represented by SEQ ID NO: 240, the first allele-specific forward primer represented by SEQ ID NO: 241 for SK03_237 Primers, the second allele-specific forward primer represented by SEQ ID NO: 242 and the reverse primer set represented by SEQ ID NO: 243, the first allele-specific forward primer represented by SEQ ID NO: 244 for SK03_245, SEQ ID NO: 245 A second allele-specific forward primer and a set of reverse primers represented by SEQ ID NO: 246, a first allele-specific forward primer represented by SEQ ID NO: 247 for SK03_252, a second allele-specific forward primer represented by SEQ ID NO: 248, and A reverse primer set represented by SEQ ID NO: 249, a first allele-specific forward primer represented by SEQ ID NO: 250 for SK03_258, a second allele-specific forward primer represented by SEQ ID NO: 251, and a reverse primer represented by SEQ ID NO: 252 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 253 for SK04_008, a second allele-specific forward primer represented by SEQ ID NO: 254, and a reverse primer set represented by SEQ ID NO: 255, SEQ ID NO: 255 The first allele-specific forward primer represented by SEQ ID NO: 256, the second allele-specific forward primer represented by SEQ ID NO: 257, and the reverse primer set represented by SEQ ID NO: 258, the first allele represented by SEQ ID NO: 259 for SK04_023 A specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 260 and a set of reverse primers represented by SEQ ID NO: 261, a first allele-specific forward primer represented by SEQ ID NO: 262 for SK04_029, SEQ ID NO: 263 The second allele-specific forward primer and the reverse primer set represented by SEQ ID NO: 264, the first allele-specific forward primer represented by SEQ ID NO: 265 for SK04_045, and the second allele-specific forward primer represented by SEQ ID NO: 266 Primers and a set of reverse primers represented by SEQ ID NO: 267, a first allele-specific forward primer represented by SEQ ID NO: 268 for SK04_051, a second allele-specific forward primer represented by SEQ ID NO: 269, and SEQ ID NO: 270 The reverse primer set, the first allele-specific forward primer represented by SEQ ID NO: 271 for SK04_060, the second allele-specific forward primer represented by SEQ ID NO: 272, and the reverse primer set represented by SEQ ID NO: 273, for SK04_069 A set of first allele-specific forward primers represented by SEQ ID NO: 274, second allele-specific forward primers represented by SEQ ID NO: 275 and reverse primers represented by SEQ ID NO: 276, the first set represented by SEQ ID NO: 277 for SK04_080 Allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 278 and the set of reverse primers represented by SEQ ID NO: 279, the first allele-specific forward primer represented by SEQ ID NO: 280 for SK04_090, SEQ ID NO: The second allele-specific forward primer represented by SEQ ID NO: 281 and the reverse primer set represented by SEQ ID NO: 282, the first allele-specific forward primer represented by SEQ ID NO: 283 for SK04_098, the second allele represented by SEQ ID NO: 284 A specific forward primer and a set of reverse primers represented by SEQ ID NO: 285, a first allele-specific forward primer represented by SEQ ID NO: 286 for SK04_107, a second allele-specific forward primer represented by SEQ ID NO: 287, and SEQ ID NO: 288 The reverse primer set represented by, the first allele-specific forward primer represented by SEQ ID NO: 289 for SK04_114, the second allele-specific forward primer represented by SEQ ID NO: 290, and the reverse primer set represented by SEQ ID NO: 291, SK04_122 The first allele-specific forward primer represented by SEQ ID NO: 292, the second allele-specific forward primer represented by SEQ ID NO: 293, and the reverse primer set represented by SEQ ID NO: 294, SEQ ID NO: 295 for SK04_129 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 296 and the reverse primer set represented by SEQ ID NO: 297, the first allele-specific forward primer represented by SEQ ID NO: 298 for SK04_138, The second allele-specific forward primer represented by SEQ ID NO: 299 and the reverse primer set represented by SEQ ID NO: 300, the first allele-specific forward primer represented by SEQ ID NO: 301 for SK04_143, and the second allele-specific forward primer represented by SEQ ID NO: 302 Allele-specific forward primer and reverse primer set represented by SEQ ID NO: 303, first allele-specific forward primer represented by SEQ ID NO: 304 for SK04_149, second allele-specific forward primer represented by SEQ ID NO: 305 and SEQ ID NO: A reverse primer set represented by SEQ ID NO: 306, a first allele-specific forward primer represented by SEQ ID NO: 307 for SK04_158, a second allele-specific forward primer represented by SEQ ID NO: 308, and a reverse primer set represented by SEQ ID NO: 309 , the first allele-specific forward primer represented by SEQ ID NO: 310 for SK04_164, the second allele-specific forward primer represented by SEQ ID NO: 311 and the reverse primer set represented by SEQ ID NO: 312, SEQ ID NO: 313 for SK04_174 The first allele-specific forward primer represented by SEQ ID NO: 314, the second allele-specific forward primer represented by SEQ ID NO: 315, and the set of reverse primers represented by SEQ ID NO: 315, the first allele-specific forward primer represented by SEQ ID NO: 316 for SK04_180 Primers, the second allele-specific forward primer represented by SEQ ID NO: 317 and the reverse primer set represented by SEQ ID NO: 318, the first allele-specific forward primer represented by SEQ ID NO: 319 for SK04_183, SEQ ID NO: 320 A second allele-specific forward primer and a set of reverse primers represented by SEQ ID NO: 321, a first allele-specific forward primer represented by SEQ ID NO: 322 for SK04_189, a second allele-specific forward primer represented by SEQ ID NO: 323, and A reverse primer set represented by SEQ ID NO: 324, a first allele-specific forward primer represented by SEQ ID NO: 325 for SK04_195, a second allele-specific forward primer represented by SEQ ID NO: 326, and a reverse primer represented by SEQ ID NO: 327 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 328 for SK04_200, a second allele-specific forward primer represented by SEQ ID NO: 329, and a reverse primer set represented by SEQ ID NO: 330, SEQ ID NO: The first allele-specific forward primer represented by SEQ ID NO: 331, the second allele-specific forward primer represented by SEQ ID NO: 332, and the reverse primer set represented by SEQ ID NO: 333, the first allele represented by SEQ ID NO: 334 for SK05_003 A specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 335 and a set of reverse primers represented by SEQ ID NO: 336, a first allele-specific forward primer represented by SEQ ID NO: 337 for SK05_010, SEQ ID NO: 338 The second allele-specific forward primer and the reverse primer set represented by SEQ ID NO: 339, the first allele-specific forward primer represented by SEQ ID NO: 340 for SK05_015, and the second allele-specific forward primer represented by SEQ ID NO: 341 Primers and a set of reverse primers represented by SEQ ID NO: 342, a first allele-specific forward primer represented by SEQ ID NO: 343 for SK05_017, a second allele-specific forward primer represented by SEQ ID NO: 344, and SEQ ID NO: 345 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 346 for SK05_032, a second allele-specific forward primer represented by SEQ ID NO: 347, and a reverse primer set represented by SEQ ID NO: 348, for SK05_036 A set of first allele-specific forward primers represented by SEQ ID NO: 349, second allele-specific forward primers represented by SEQ ID NO: 350 and reverse primers represented by SEQ ID NO: 351, the first set represented by SEQ ID NO: 352 for SK05_039 Allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 353 and the set of reverse primers represented by SEQ ID NO: 354, the first allele-specific forward primer represented by SEQ ID NO: 355 for SK05_059, SEQ ID NO: The second allele-specific forward primer represented by SEQ ID NO: 356 and the reverse primer set represented by SEQ ID NO: 357, the first allele-specific forward primer represented by SEQ ID NO: 358 for SK05_068, the second allele represented by SEQ ID NO: 359 A set of specific forward primers and reverse primers represented by SEQ ID NO: 360, a first allele-specific forward primer represented by SEQ ID NO: 361 for SK05_075, a second allele-specific forward primer represented by SEQ ID NO: 362, and SEQ ID NO: 363. The reverse primer set represented by, the first allele-specific forward primer represented by SEQ ID NO: 364 for SK05_087, the second allele-specific forward primer represented by SEQ ID NO: 365, and the reverse primer set represented by SEQ ID NO: 366, SK05_092 The first allele-specific forward primer represented by SEQ ID NO: 367, the second allele-specific forward primer represented by SEQ ID NO: 368, and the reverse primer set represented by SEQ ID NO: 369, SEQ ID NO: 370 for SK05_100 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 371 and the reverse primer set represented by SEQ ID NO: 372, the first allele-specific forward primer represented by SEQ ID NO: 373 for SK05_108, The second allele-specific forward primer represented by SEQ ID NO: 374 and the reverse primer set represented by SEQ ID NO: 375, the first allele-specific forward primer represented by SEQ ID NO: 374 for SK05_112, the second allele-specific forward primer represented by SEQ ID NO: 375 Allele-specific forward primer and reverse primer set represented by SEQ ID NO: 376, first allele-specific forward primer represented by SEQ ID NO: 379 for SK05_115, second allele-specific forward primer represented by SEQ ID NO: 380 and SEQ ID NO: A reverse primer set represented by 381, a first allele-specific forward primer represented by SEQ ID NO: 382 for SK05_122, a second allele-specific forward primer represented by SEQ ID NO: 383, and a reverse primer set represented by SEQ ID NO: 384 , the first allele-specific forward primer represented by SEQ ID NO: 385 for SK05_132, the second allele-specific forward primer represented by SEQ ID NO: 386 and the reverse primer set represented by SEQ ID NO: 387, SEQ ID NO: 388 for SK05_137 The first allele-specific forward primer represented by SEQ ID NO: 389, the second allele-specific forward primer represented by SEQ ID NO: 390, and the set of reverse primers represented by SEQ ID NO: 390, the first allele-specific forward primer represented by SEQ ID NO: 391 for SK05_148 Primers, the second allele-specific forward primer represented by SEQ ID NO: 392 and the reverse primer set represented by SEQ ID NO: 393, the first allele-specific forward primer represented by SEQ ID NO: 394 for SK05_155, SEQ ID NO: 395 A second allele-specific forward primer and a set of reverse primers represented by SEQ ID NO: 396, a first allele-specific forward primer represented by SEQ ID NO: 397 for SK05_162, a second allele-specific forward primer represented by SEQ ID NO: 398, and A reverse primer set represented by SEQ ID NO: 399, a first allele-specific forward primer represented by SEQ ID NO: 400 for SK06_006, a second allele-specific forward primer represented by SEQ ID NO: 401, and a reverse primer represented by SEQ ID NO: 402 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 403 for SK06_013, a second allele-specific forward primer represented by SEQ ID NO: 404, and a reverse primer set represented by SEQ ID NO: 405, SEQ ID NO: SK06_022 The first allele-specific forward primer represented by SEQ ID NO: 406, the second allele-specific forward primer represented by SEQ ID NO: 407, and the reverse primer set represented by SEQ ID NO: 408, the first allele represented by SEQ ID NO: 409 for SK06_029 A specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 410 and a set of reverse primers represented by SEQ ID NO: 411, a first allele-specific forward primer represented by SEQ ID NO: 412 for SK06_035, SEQ ID NO: 413 The second allele-specific forward primer and the reverse primer set represented by SEQ ID NO: 414, the first allele-specific forward primer represented by SEQ ID NO: 415 for SK06_042, and the second allele-specific forward primer represented by SEQ ID NO: 416 Primers and a set of reverse primers represented by SEQ ID NO: 417, a first allele-specific forward primer represented by SEQ ID NO: 418 for SK06_048, a second allele-specific forward primer represented by SEQ ID NO: 419, and SEQ ID NO: 420 The reverse primer set, the first allele-specific forward primer represented by SEQ ID NO: 421 for SK06_056, the second allele-specific forward primer represented by SEQ ID NO: 422, and the reverse primer set represented by SEQ ID NO: 423, for SK06_063 A set of first allele-specific forward primers represented by SEQ ID NO: 424, second allele-specific forward primers represented by SEQ ID NO: 425, and reverse primers represented by SEQ ID NO: 426, the first set represented by SEQ ID NO: 427 for SK06_067 Allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 428 and the reverse primer set represented by SEQ ID NO: 429, the first allele-specific forward primer represented by SEQ ID NO: 430 for SK06_075, SEQ ID NO: The second allele-specific forward primer represented by 431 and the reverse primer set represented by SEQ ID NO: 432, the first allele-specific forward primer represented by SEQ ID NO: 433 for SK06_081, the second allele represented by SEQ ID NO: 434 A set of specific forward primers and reverse primers represented by SEQ ID NO: 435, a first allele-specific forward primer represented by SEQ ID NO: 436 for SK06_098, a second allele-specific forward primer represented by SEQ ID NO: 437, and SEQ ID NO: 438. The reverse primer set represented by, the first allele-specific forward primer represented by SEQ ID NO: 439 for SK06_105, the second allele-specific forward primer represented by SEQ ID NO: 440, and the reverse primer set represented by SEQ ID NO: 441, SK06_113 The first allele-specific forward primer represented by SEQ ID NO: 442, the second allele-specific forward primer represented by SEQ ID NO: 443, and the reverse primer set represented by SEQ ID NO: 444, SEQ ID NO: 445 for SK06_121 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 446 and the reverse primer set represented by SEQ ID NO: 447, the first allele-specific forward primer represented by SEQ ID NO: 448 for SK06_129, The second allele-specific forward primer represented by SEQ ID NO: 449 and the reverse primer set represented by SEQ ID NO: 450, the first allele-specific forward primer represented by SEQ ID NO: 451 for SK06_136, the second allele-specific forward primer represented by SEQ ID NO: 452 An allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 453, a first allele-specific forward primer represented by SEQ ID NO: 454 for SK06_139, a second allele-specific forward primer represented by SEQ ID NO: 455, and SEQ ID NO: A reverse primer set represented by SEQ ID NO: 456, a first allele-specific forward primer represented by SEQ ID NO: 457 for SK06_145, a second allele-specific forward primer represented by SEQ ID NO: 458, and a reverse primer set represented by SEQ ID NO: 459 , the first allele-specific forward primer represented by SEQ ID NO: 460 for SK06_152, the second allele-specific forward primer represented by SEQ ID NO: 461 and the reverse primer set represented by SEQ ID NO: 462, SEQ ID NO: 463 for SK06_157 The first allele-specific forward primer represented by SEQ ID NO: 464, the second allele-specific forward primer represented by SEQ ID NO: 465, and the set of reverse primers represented by SEQ ID NO: 465, the first allele-specific forward primer represented by SEQ ID NO: 466 for SK06_162 Primers, the second allele-specific forward primer represented by SEQ ID NO: 467 and the reverse primer set represented by SEQ ID NO: 468, the first allele-specific forward primer represented by SEQ ID NO: 469 for SK06_170, SEQ ID NO: 470 A second allele-specific forward primer and a set of reverse primers represented by SEQ ID NO: 471, a first allele-specific forward primer represented by SEQ ID NO: 472 for SK06_177, a second allele-specific forward primer represented by SEQ ID NO: 473, and A reverse primer set represented by SEQ ID NO: 474, a first allele-specific forward primer represented by SEQ ID NO: 475 for SK06_183, a second allele-specific forward primer represented by SEQ ID NO: 476, and a reverse primer represented by SEQ ID NO: 477 Primer set, a first allele-specific forward primer represented by SEQ ID NO: 478 for SK06_190, a second allele-specific forward primer represented by SEQ ID NO: 479, and a reverse primer set represented by SEQ ID NO: 480, SEQ ID NO: The first allele-specific forward primer represented by SEQ ID NO: 481, the second allele-specific forward primer represented by SEQ ID NO: 482, and the reverse primer set represented by SEQ ID NO: 483, the first allele represented by SEQ ID NO: 484 for SK06_198 A specific forward primer, a second allele-specific forward primer represented by SEQ ID NO: 485 and a set of reverse primers represented by SEQ ID NO: 486, a first allele-specific forward primer represented by SEQ ID NO: 487 for SK06_205, SEQ ID NO: 488 The second allele-specific forward primer and the reverse primer set represented by SEQ ID NO: 489, the first allele-specific forward primer represented by SEQ ID NO: 490 for SK06_212, and the second allele-specific forward primer represented by SEQ ID NO: 491 Primers and a set of reverse primers represented by SEQ ID NO: 492, a first allele-specific forward primer represented by SEQ ID NO: 493 for SK06_222, a second allele-specific forward primer represented by SEQ ID NO: 494, and SEQ ID NO: 495 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 496 for SK06_235, a second allele-specific forward primer represented by SEQ ID NO: 497, and a reverse primer set represented by SEQ ID NO: 498, for SK06_240 A set of first allele-specific forward primers represented by SEQ ID NO: 499, second allele-specific forward primers represented by SEQ ID NO: 500 and reverse primers represented by SEQ ID NO: 501, the first set represented by SEQ ID NO: 502 for SK06_249 Allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 503 and the reverse primer set represented by SEQ ID NO: 504, the first allele-specific forward primer represented by SEQ ID NO: 505 for SK06_256, SEQ ID NO: The second allele-specific forward primer represented by SEQ ID NO: 506 and the reverse primer set represented by SEQ ID NO: 507, the first allele-specific forward primer represented by SEQ ID NO: 508 for SK07_006, the second allele represented by SEQ ID NO: 509 A specific forward primer and a set of reverse primers represented by SEQ ID NO: 510, a first allele-specific forward primer represented by SEQ ID NO: 511 for SK07_022, a second allele-specific forward primer represented by SEQ ID NO: 512, and SEQ ID NO: 513 The reverse primer set represented by, the first allele-specific forward primer represented by SEQ ID NO: 514 for SK07_043, the second allele-specific forward primer represented by SEQ ID NO: 515, and the reverse primer set represented by SEQ ID NO: 516, SK07_047 The first allele-specific forward primer represented by SEQ ID NO: 517, the second allele-specific forward primer represented by SEQ ID NO: 518, and the reverse primer set represented by SEQ ID NO: 519, SEQ ID NO: 520 for SK07_055 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 521 and the reverse primer set represented by SEQ ID NO: 522, the first allele-specific forward primer represented by SEQ ID NO: 523 for SK07_064, The second allele-specific forward primer represented by SEQ ID NO: 524 and the reverse primer set represented by SEQ ID NO: 525, the first allele-specific forward primer represented by SEQ ID NO: 526 for SK07_073, the second allele-specific forward primer represented by SEQ ID NO: 527 An allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 528, a first allele-specific forward primer represented by SEQ ID NO: 529 for SK07_079, a second allele-specific forward primer represented by SEQ ID NO: 530, and SEQ ID NO: A reverse primer set represented by 531, a first allele-specific forward primer represented by SEQ ID NO: 532 for SK07_083, a second allele-specific forward primer represented by SEQ ID NO: 533, and a reverse primer set represented by SEQ ID NO: 534 , the first allele-specific forward primer represented by SEQ ID NO: 535 for SK07_094, the second allele-specific forward primer represented by SEQ ID NO: 536 and the reverse primer set represented by SEQ ID NO: 537, SEQ ID NO: 538 for SK07_100. The first allele-specific forward primer represented by SEQ ID NO: 539, the second allele-specific forward primer represented by SEQ ID NO: 540, and the reverse primer set represented by SEQ ID NO: 541 for SK07_106. Primers, the second allele-specific forward primer represented by SEQ ID NO: 542 and the reverse primer set represented by SEQ ID NO: 543, the first allele-specific forward primer represented by SEQ ID NO: 544 for SK07_114, the first allele-specific forward primer represented by SEQ ID NO: 545 2 allele-specific forward primers and reverse primer set represented by SEQ ID NO: 546, the first allele-specific forward primer represented by SEQ ID NO: 547 for SK07_126, the second allele-specific forward primer represented by SEQ ID NO: 548 and sequence Reverse primer set represented by No. 549, the first allele-specific forward primer represented by SEQ ID NO: 550 for SK07_133, the second allele-specific forward primer represented by SEQ ID NO: 551, and the reverse primer represented by SEQ ID NO: 552 Set, a first allele specific forward primer represented by SEQ ID NO: 553 to SK07_130, a second allele specific forward primer represented by SEQ ID NO: 554 and a reverse primer set represented by SEQ ID NO: 555, SEQ ID NO: 556 to SK07_145 The first allele-specific forward primer represented by SEQ ID NO: 557, the second allele-specific forward primer represented by SEQ ID NO: 558, and the reverse primer set represented by SEQ ID NO: 558, the first allele-specific represented by SEQ ID NO: 559 for SK07_151 Forward primer, a second allele-specific forward primer represented by SEQ ID NO: 560 and a set of reverse primers represented by SEQ ID NO: 561, a first allele-specific forward primer represented by SEQ ID NO: 562 for SK07_164, SEQ ID NO: 563 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 564, a first allele-specific forward primer represented by SEQ ID NO: 565 for SK07_166, and a second allele-specific forward primer represented by SEQ ID NO: 566 and a set of reverse primers represented by SEQ ID NO: 567, a first allele-specific forward primer represented by SEQ ID NO: 568 for SK08_001, a second allele-specific forward primer represented by SEQ ID NO: 569, and a reverse sequence represented by SEQ ID NO: 570. The anti-primer set, the first allele-specific forward primer represented by SEQ ID NO: 571 for SK08_008, the second allele-specific forward primer represented by SEQ ID NO: 572, and the reverse primer set represented by SEQ ID NO: 573, the sequence for SK08_012 The first allele-specific forward primer represented by SEQ ID NO: 574, the second allele-specific forward primer represented by SEQ ID NO: 575, and the reverse primer set represented by SEQ ID NO: 576, the first allele represented by SEQ ID NO: 577 for SK08_020 Trait specific forward primer, second allele specific forward primer represented by SEQ ID NO: 578 and reverse primer set represented by SEQ ID NO: 579, first allele specific forward primer represented by SEQ ID NO: 580 for SK08_032, SEQ ID NO: 581 A second allele-specific forward primer represented by and a reverse primer set represented by SEQ ID NO: 582, a first allele-specific forward primer represented by SEQ ID NO: 583 for SK08_039, and a second allele-specific represented by SEQ ID NO: 584 A forward primer and a reverse primer set represented by SEQ ID NO: 585, a first allele-specific forward primer represented by SEQ ID NO: 586 for SK08_046, a second allele-specific forward primer represented by SEQ ID NO: 587, and SEQ ID NO: 588 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 589 for SK08_052, a second allele-specific forward primer represented by SEQ ID NO: 590, and a reverse primer set represented by SEQ ID NO: 591, SK08_066 The first allele-specific forward primer represented by SEQ ID NO: 592, the second allele-specific forward primer represented by SEQ ID NO: 593, and the reverse primer set represented by SEQ ID NO: 594, the first allele-specific forward primer represented by SEQ ID NO: 595 for SK08_072 1 allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 596 and the reverse primer set represented by SEQ ID NO: 597, the first allele-specific forward primer represented by SEQ ID NO: 598 for SK08_125, sequence The second allele-specific forward primer represented by No. 599 and the reverse primer set represented by SEQ ID NO: 600, the first allele-specific forward primer represented by SEQ ID NO: 601 for SK08_120, the second allele represented by SEQ ID NO: 602 A trait-specific forward primer and a set of reverse primers represented by SEQ ID NO: 603, a first allele-specific forward primer represented by SEQ ID NO: 604 for SK08_137, a second allele-specific forward primer represented by SEQ ID NO: 605, and SEQ ID NO: 606 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 607 for SK08_142, a second allele-specific forward primer represented by SEQ ID NO: 608, and a reverse primer set represented by SEQ ID NO: 609, A set of a first allele-specific forward primer represented by SEQ ID NO: 610 for SK08_148, a second allele-specific forward primer represented by SEQ ID NO: 611 and a reverse primer set represented by SEQ ID NO: 612, SEQ ID NO: 613 for SK08_156 A set of first allele-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 614 and a reverse primer set represented by SEQ ID NO: 615, the first allele-specific forward primer represented by SEQ ID NO: 616 for SK08_164 , the second allele-specific forward primer represented by SEQ ID NO: 617 and the reverse primer set represented by SEQ ID NO: 618, the first allele-specific forward primer represented by SEQ ID NO: 619 for SK08_172, the first allele-specific forward primer represented by SEQ ID NO: 620 2 allele-specific forward primers and reverse primer set represented by SEQ ID NO: 621, the first allele-specific forward primer represented by SEQ ID NO: 622 for SK08_179, the second allele-specific forward primer represented by SEQ ID NO: 623 and sequence Reverse primer set represented by No. 624, the first allele-specific forward primer represented by SEQ ID NO: 625 for SK08_187, the second allele-specific forward primer represented by SEQ ID NO: 626, and the reverse primer represented by SEQ ID NO: 627 Set, first allele specific forward primer represented by SEQ ID NO: 628 for SK08_197, second allele specific forward primer represented by SEQ ID NO: 629 and reverse primer set represented by SEQ ID NO: 630, SEQ ID NO: 631 for SK08_203 The first allele-specific forward primer represented by SEQ ID NO: 632, the second allele-specific forward primer represented by SEQ ID NO: 633, and the reverse primer set represented by SEQ ID NO: 633, the first allele-specific represented by SEQ ID NO: 634 for SK08_209 Forward primer, a second allele-specific forward primer represented by SEQ ID NO: 635 and a set of reverse primers represented by SEQ ID NO: 636, a first allele-specific forward primer represented by SEQ ID NO: 637 for SK08_216, SEQ ID NO: 638 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 639, a first allele-specific forward primer represented by SEQ ID NO: 640 for SK08_219, and a second allele-specific forward primer represented by SEQ ID NO: 641 and a reverse primer set represented by SEQ ID NO: 642, a first allele-specific forward primer represented by SEQ ID NO: 643 for SK08_227, a second allele-specific forward primer represented by SEQ ID NO: 644, and a reverse sequence represented by SEQ ID NO: 645. The anti-primer set, the first allele-specific forward primer represented by SEQ ID NO: 646 for SK08_233, the second allele-specific forward primer represented by SEQ ID NO: 647, and the reverse primer set represented by SEQ ID NO: 648, the sequence for SK08_241 A first allele-specific forward primer represented by SEQ ID NO: 649, a second allele-specific forward primer represented by SEQ ID NO: 650, and a reverse primer set represented by SEQ ID NO: 651, the first allele represented by SEQ ID NO: 652 for SK08_254 A set of trait-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 653 and a reverse primer set represented by SEQ ID NO: 654, a first allele-specific forward primer represented by SEQ ID NO: 655 for SK08_260, SEQ ID NO: 656 A second allele-specific forward primer represented by and a reverse primer set represented by SEQ ID NO: 657, a first allele-specific forward primer represented by SEQ ID NO: 658 for SK09_004, and a second allele-specific represented by SEQ ID NO: 659 A forward primer and a reverse primer set represented by SEQ ID NO: 660, a first allele-specific forward primer represented by SEQ ID NO: 661 for SK09_028, a second allele-specific forward primer represented by SEQ ID NO: 662, and SEQ ID NO: 663 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 664 for SK09_035, a second allele-specific forward primer represented by SEQ ID NO: 665, and a reverse primer set represented by SEQ ID NO: 666, to SK09_042 The first allele-specific forward primer represented by SEQ ID NO: 667, the second allele-specific forward primer represented by SEQ ID NO: 668, and the reverse primer set represented by SEQ ID NO: 669, the first allele-specific forward primer represented by SEQ ID NO: 670 for SK09_052 1 allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 671 and the reverse primer set represented by SEQ ID NO: 672, the first allele-specific forward primer represented by SEQ ID NO: 673 for SK09_056, sequence The second allele-specific forward primer represented by SEQ ID NO: 674 and the reverse primer set represented by SEQ ID NO: 675, the first allele-specific forward primer represented by SEQ ID NO: 676 for SK09_062, the second allele represented by SEQ ID NO: 677 A trait-specific forward primer and a reverse primer set represented by SEQ ID NO: 678, a first allele-specific forward primer represented by SEQ ID NO: 679 for SK09_068, a second allele-specific forward primer represented by SEQ ID NO: 680, and SEQ ID NO: 681 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 682 for SK09_075, a second allele-specific forward primer represented by SEQ ID NO: 683, and a reverse primer set represented by SEQ ID NO: 684, A set of a first allele-specific forward primer represented by SEQ ID NO: 685 for SK09_093, a second allele-specific forward primer represented by SEQ ID NO: 686 and a reverse primer set represented by SEQ ID NO: 687, SEQ ID NO: 688 for SK09_123 A set of first allele-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 689 and a reverse primer set represented by SEQ ID NO: 690, the first allele-specific forward primer represented by SEQ ID NO: 691 for SK09_132 , the second allele-specific forward primer represented by SEQ ID NO: 692 and the reverse primer set represented by SEQ ID NO: 693, the first allele-specific forward primer represented by SEQ ID NO: 694 for SK09_140, the first allele-specific forward primer represented by SEQ ID NO: 695 2 allele-specific forward primers and reverse primer set represented by SEQ ID NO: 696, the first allele-specific forward primer represented by SEQ ID NO: 697 for SK09_154, the second allele-specific forward primer represented by SEQ ID NO: 698 and sequence Reverse primer set represented by No. 699, the first allele-specific forward primer represented by SEQ ID NO: 700 for SK09_162, the second allele-specific forward primer represented by SEQ ID NO: 701, and the reverse primer represented by SEQ ID NO: 702 Set, a first allele specific forward primer represented by SEQ ID NO: 703 for SK09_160, a second allele specific forward primer represented by SEQ ID NO: 704 and a reverse primer set represented by SEQ ID NO: 705, SEQ ID NO: 706 for SK09_169 The first allele-specific forward primer represented by SEQ ID NO: 707, the second allele-specific forward primer represented by SEQ ID NO: 708, and the reverse primer set represented by SEQ ID NO: 708, the first allele-specific represented by SEQ ID NO: 709 for SK09_173 Forward primer, second allele-specific forward primer represented by SEQ ID NO: 710 and reverse primer set represented by SEQ ID NO: 711, first allele-specific forward primer represented by SEQ ID NO: 712 for SK09_177, SEQ ID NO: 713 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 714, a first allele-specific forward primer represented by SEQ ID NO: 715 for SK09_181, and a second allele-specific forward primer represented by SEQ ID NO: 716 and a reverse primer set represented by SEQ ID NO: 717, a first allele-specific forward primer represented by SEQ ID NO: 718 for SK09_186, a second allele-specific forward primer represented by SEQ ID NO: 719, and a reverse sequence represented by SEQ ID NO: 720. The anti-primer set, the first allele-specific forward primer represented by SEQ ID NO: 721 for SK09_189, the second allele-specific forward primer represented by SEQ ID NO: 722, and the reverse primer set represented by SEQ ID NO: 723, the sequence for SK09_197 The first allele-specific forward primer represented by SEQ ID NO: 724, the second allele-specific forward primer represented by SEQ ID NO: 725, and the reverse primer set represented by SEQ ID NO: 726, the first allele represented by SEQ ID NO: 727 for SK09_208 Trait specific forward primer, second allele specific forward primer represented by SEQ ID NO: 728 and reverse primer set represented by SEQ ID NO: 729, first allele specific forward primer represented by SEQ ID NO: 730 for SK09_213, SEQ ID NO: 731 A second allele-specific forward primer represented by and a reverse primer set represented by SEQ ID NO: 732, a first allele-specific forward primer represented by SEQ ID NO: 733 for SK09_224, and a second allele-specific represented by SEQ ID NO: 734 A forward primer and a reverse primer set represented by SEQ ID NO: 735, a first allele-specific forward primer represented by SEQ ID NO: 736 for SK09_228, a second allele-specific forward primer represented by SEQ ID NO: 737, and SEQ ID NO: 738 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 739 for SK10_005, a second allele-specific forward primer represented by SEQ ID NO: 740, and a reverse primer set represented by SEQ ID NO: 741, SK10_011 The first allele-specific forward primer represented by SEQ ID NO: 742, the second allele-specific forward primer represented by SEQ ID NO: 743, and the reverse primer set represented by SEQ ID NO: 744, the first allele-specific forward primer represented by SEQ ID NO: 745 for SK10_014 1 allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 746 and the reverse primer set represented by SEQ ID NO: 747, the first allele-specific forward primer represented by SEQ ID NO: 748 for SK10_019, sequence The second allele-specific forward primer represented by No. 749 and the reverse primer set represented by SEQ ID NO: 750, the first allele-specific forward primer represented by SEQ ID NO: 751 for SK10_028, the second allele represented by SEQ ID NO: 752 A trait-specific forward primer and a reverse primer set represented by SEQ ID NO: 753, a first allele-specific forward primer represented by SEQ ID NO: 754 for SK10_032, a second allele-specific forward primer represented by SEQ ID NO: 755, and SEQ ID NO: 756 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 757 for SK10_038, a second allele-specific forward primer represented by SEQ ID NO: 758, and a reverse primer set represented by SEQ ID NO: 759, A first allele-specific forward primer represented by SEQ ID NO: 760 for SK10_046, a second allele-specific forward primer represented by SEQ ID NO: 761 and a reverse primer set represented by SEQ ID NO: 762, SEQ ID NO: 763 for SK10_053 A set of first allele-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 764 and a reverse primer set represented by SEQ ID NO: 765, the first allele-specific forward primer represented by SEQ ID NO: 766 for SK10_061 , the second allele-specific forward primer represented by SEQ ID NO: 757 and the reverse primer set represented by SEQ ID NO: 768, the first allele-specific forward primer represented by SEQ ID NO: 769 for SK10_076, the first allele-specific forward primer represented by SEQ ID NO: 770 2 allele-specific forward primers and reverse primer set represented by SEQ ID NO: 771, the first allele-specific forward primer represented by SEQ ID NO: 772 for SK10_086, the second allele-specific forward primer represented by SEQ ID NO: 773 and sequence Reverse primer set represented by No. 774, the first allele-specific forward primer represented by SEQ ID NO: 775 for SK10_092, the second allele-specific forward primer represented by SEQ ID NO: 776, and the reverse primer represented by SEQ ID NO: 777 Set, a first allele specific forward primer represented by SEQ ID NO: 778 for SK10_097, a second allele specific forward primer represented by SEQ ID NO: 779 and a reverse primer set represented by SEQ ID NO: 780, SEQ ID NO: 781 for SK10_113 The first allele-specific forward primer represented by SEQ ID NO: 782, the second allele-specific forward primer represented by SEQ ID NO: 783, and the reverse primer set represented by SEQ ID NO: 783, the first allele-specific represented by SEQ ID NO: 784 for SK10_123 Forward primer, a second allele-specific forward primer represented by SEQ ID NO: 785 and a set of reverse primers represented by SEQ ID NO: 786, a first allele-specific forward primer represented by SEQ ID NO: 787 for SK10_128, SEQ ID NO: 788 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 789, a first allele-specific forward primer represented by SEQ ID NO: 790 for SK10_131, and a second allele-specific forward primer represented by SEQ ID NO: 791 and a reverse primer set represented by SEQ ID NO: 792, a first allele-specific forward primer represented by SEQ ID NO: 793 for SK10_135, a second allele-specific forward primer represented by SEQ ID NO: 794, and a reverse sequence represented by SEQ ID NO: 795. The anti-primer set, the first allele-specific forward primer represented by SEQ ID NO: 796 for SK10_146, the second allele-specific forward primer represented by SEQ ID NO: 797, and the reverse primer set represented by SEQ ID NO: 798, the sequence for SK10_153 The first allele-specific forward primer represented by SEQ ID NO: 799, the second allele-specific forward primer represented by SEQ ID NO: 800, and the reverse primer set represented by SEQ ID NO: 801, the first allele represented by SEQ ID NO: 802 for SK10_161 Trait specific forward primer, second allele specific forward primer represented by SEQ ID NO: 803 and reverse primer set represented by SEQ ID NO: 804, first allele specific forward primer represented by SEQ ID NO: 805 for SK10_167, SEQ ID NO: 806 A second allele-specific forward primer represented by and a reverse primer set represented by SEQ ID NO: 807, a first allele-specific forward primer represented by SEQ ID NO: 808 for SK10_174, and a second allele-specific represented by SEQ ID NO: 809 A forward primer and a reverse primer set represented by SEQ ID NO: 810, a first allele-specific forward primer represented by SEQ ID NO: 811 for SK10_182, a second allele-specific forward primer represented by SEQ ID NO: 812, and SEQ ID NO: 813 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 814 for SK11_001, a second allele-specific forward primer represented by SEQ ID NO: 815, and a reverse primer set represented by SEQ ID NO: 816, SK11_007 The first allele-specific forward primer represented by SEQ ID NO: 817, the second allele-specific forward primer represented by SEQ ID NO: 818, and the reverse primer set represented by SEQ ID NO: 819, the first allele-specific forward primer represented by SEQ ID NO: 820 for SK11_015 1 allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 821 and the reverse primer set represented by SEQ ID NO: 822, the first allele-specific forward primer represented by SEQ ID NO: 823 for SK11_022, sequence The second allele-specific forward primer represented by SEQ ID NO: 824 and the reverse primer set represented by SEQ ID NO: 825, the first allele-specific forward primer represented by SEQ ID NO: 826 for SK11_027, the second allele represented by SEQ ID NO: 827 A trait-specific forward primer and a set of reverse primers represented by SEQ ID NO: 828, a first allele-specific forward primer represented by SEQ ID NO: 829 for SK11_035, a second allele-specific forward primer represented by SEQ ID NO: 830, and SEQ ID NO: 831 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 832 for SK11_042, a second allele-specific forward primer represented by SEQ ID NO: 833, and a reverse primer set represented by SEQ ID NO: 834, A set of a first allele-specific forward primer represented by SEQ ID NO: 835 for SK11_050, a second allele-specific forward primer represented by SEQ ID NO: 836 and a reverse primer set represented by SEQ ID NO: 837, SEQ ID NO: 838 for SK11_059 A set of first allele-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 839 and a reverse primer set represented by SEQ ID NO: 840, the first allele-specific forward primer represented by SEQ ID NO: 841 for SK11_066 , the second allele-specific forward primer represented by SEQ ID NO: 842 and the reverse primer set represented by SEQ ID NO: 843, the first allele-specific forward primer represented by SEQ ID NO: 844 for SK11_073, the first allele-specific forward primer represented by SEQ ID NO: 845 2 allele-specific forward primers and reverse primer set represented by SEQ ID NO: 846, the first allele-specific forward primer represented by SEQ ID NO: 847 for SK11_084, the second allele-specific forward primer represented by SEQ ID NO: 848 and sequence Reverse primer set represented by No. 849, the first allele-specific forward primer represented by SEQ ID NO: 850 for SK11_092, the second allele-specific forward primer represented by SEQ ID NO: 851, and the reverse primer represented by SEQ ID NO: 852 Set, a first allele specific forward primer represented by SEQ ID NO: 853 for SK11_102, a second allele specific forward primer represented by SEQ ID NO: 854 and a reverse primer set represented by SEQ ID NO: 855, SEQ ID NO: 856 for SK11_109 The first allele-specific forward primer represented by SEQ ID NO: 857, the second allele-specific forward primer represented by SEQ ID NO: 858, and the reverse primer set represented by SEQ ID NO: 858, the first allele-specific represented by SEQ ID NO: 859 for SK11_117 Forward primer, a second allele-specific forward primer represented by SEQ ID NO: 860 and a set of reverse primers represented by SEQ ID NO: 861, a first allele-specific forward primer represented by SEQ ID NO: 862 for SK11_125, SEQ ID NO: 863 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 864, a first allele-specific forward primer represented by SEQ ID NO: 865 for SK11_130, and a second allele-specific forward primer represented by SEQ ID NO: 866 and a set of reverse primers represented by SEQ ID NO: 867, a first allele-specific forward primer represented by SEQ ID NO: 868 for SK11_137, a second allele-specific forward primer represented by SEQ ID NO: 869, and a reverse sequence represented by SEQ ID NO: 870. The anti-primer set, the first allele-specific forward primer represented by SEQ ID NO: 871 for SK12_007, the second allele-specific forward primer represented by SEQ ID NO: 872, and the reverse primer set represented by SEQ ID NO: 873, the sequence for SK12_019 A set of first allele-specific forward primers represented by SEQ ID NO: 874, second allele-specific forward primers represented by SEQ ID NO: 875 and reverse primers represented by SEQ ID NO: 876, the first allele represented by SEQ ID NO: 877 for SK12_022 A set of trait-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 878 and a set of reverse primers represented by SEQ ID NO: 879, a first allele-specific forward primer represented by SEQ ID NO: 880 for SK12_033, SEQ ID NO: 881 A second allele-specific forward primer represented by and a reverse primer set represented by SEQ ID NO: 882, a first allele-specific forward primer represented by SEQ ID NO: 883 for SK12_042, and a second allele-specific represented by SEQ ID NO: 884 A forward primer and a reverse primer set represented by SEQ ID NO: 885, a first allele-specific forward primer represented by SEQ ID NO: 886 for SK12_052, a second allele-specific forward primer represented by SEQ ID NO: 887, and SEQ ID NO: 888 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 889 for SK12_066, a second allele-specific forward primer represented by SEQ ID NO: 890, and a reverse primer set represented by SEQ ID NO: 891, SK12_073 The first allele-specific forward primer represented by SEQ ID NO: 892, the second allele-specific forward primer represented by SEQ ID NO: 893, and the reverse primer set represented by SEQ ID NO: 894, the first allele-specific forward primer represented by SEQ ID NO: 895 for SK12_080 1 allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 896 and the reverse primer set represented by SEQ ID NO: 897, the first allele-specific forward primer represented by SEQ ID NO: 898 for SK12_089, sequence The second allele-specific forward primer represented by No. 899 and the reverse primer set represented by SEQ ID NO: 900, the first allele-specific forward primer represented by SEQ ID NO: 901 for SK12_096, the second allele represented by SEQ ID NO: 902 A trait-specific forward primer and a reverse primer set represented by SEQ ID NO: 903, a first allele-specific forward primer represented by SEQ ID NO: 904 for SK12_110, a second allele-specific forward primer represented by SEQ ID NO: 905, and SEQ ID NO: 906 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 907 for SK12_118, a second allele-specific forward primer represented by SEQ ID NO: 908, and a reverse primer set represented by SEQ ID NO: 909, A first allele-specific forward primer represented by SEQ ID NO: 910 for SK12_123, a second allele-specific forward primer represented by SEQ ID NO: 911 and a reverse primer set represented by SEQ ID NO: 912, SEQ ID NO: 913 for SK12_128 A set of first allele-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 914 and a reverse primer set represented by SEQ ID NO: 915, the first allele-specific forward primer represented by SEQ ID NO: 916 for SK12_134 , the second allele-specific forward primer represented by SEQ ID NO: 917 and the reverse primer set represented by SEQ ID NO: 918, the first allele-specific forward primer represented by SEQ ID NO: 919 for SK12_138, the first allele-specific forward primer represented by SEQ ID NO: 920 2 allele-specific forward primers and reverse primer set represented by SEQ ID NO: 921, the first allele-specific forward primer represented by SEQ ID NO: 922 for SK12_147, the second allele-specific forward primer represented by SEQ ID NO: 923, and the sequence Reverse primer set represented by No. 924, the first allele-specific forward primer represented by SEQ ID NO: 925 for SK12_151, the second allele-specific forward primer represented by SEQ ID NO: 926, and the reverse primer represented by SEQ ID NO: 927 Set, first allele specific forward primer represented by SEQ ID NO: 928 to SK12_161, second allele specific forward primer represented by SEQ ID NO: 929 and reverse primer set represented by SEQ ID NO: 930, SEQ ID NO: 931 to SK13_003 The first allele-specific forward primer represented by SEQ ID NO: 932, the second allele-specific forward primer represented by SEQ ID NO: 933, and the reverse primer set represented by SEQ ID NO: 933, the first allele-specific represented by SEQ ID NO: 934 for SK13_008 Forward primer, a second allele-specific forward primer represented by SEQ ID NO: 935 and a set of reverse primers represented by SEQ ID NO: 936, a first allele-specific forward primer represented by SEQ ID NO: 937 for SK13_015, SEQ ID NO: 938 A second allele-specific forward primer and a reverse primer set represented by SEQ ID NO: 939, a first allele-specific forward primer represented by SEQ ID NO: 940 for SK13_023, and a second allele-specific forward primer represented by SEQ ID NO: 941 and a reverse primer set represented by SEQ ID NO: 942, a first allele-specific forward primer represented by SEQ ID NO: 943 for SK13_055, a second allele-specific forward primer represented by SEQ ID NO: 944, and a reverse sequence represented by SEQ ID NO: 945. The anti-primer set, the first allele-specific forward primer represented by SEQ ID NO: 946 for SK13_061, the second allele-specific forward primer represented by SEQ ID NO: 947, and the reverse primer set represented by SEQ ID NO: 948, the sequence for SK13_067 The first allele-specific forward primer represented by SEQ ID NO: 949, the second allele-specific forward primer represented by SEQ ID NO: 950, and the reverse primer set represented by SEQ ID NO: 951, the first allele represented by SEQ ID NO: 952 for SK13_071 A set of trait-specific forward primers, a second allele-specific forward primer represented by SEQ ID NO: 953 and a reverse primer set represented by SEQ ID NO: 954, a first allele-specific forward primer represented by SEQ ID NO: 955 for SK13_080, SEQ ID NO: 956 The second allele-specific forward primer represented by and the reverse primer set represented by SEQ ID NO: 957, the first allele-specific forward primer represented by SEQ ID NO: 958 for SK13_088, the second allele-specific represented by SEQ ID NO: 959 A forward primer and a reverse primer set represented by SEQ ID NO: 960, a first allele-specific forward primer represented by SEQ ID NO: 961 for SK13_094, a second allele-specific forward primer represented by SEQ ID NO: 962, and SEQ ID NO: 963 A reverse primer set, a first allele-specific forward primer represented by SEQ ID NO: 964 for SK13_103, a second allele-specific forward primer represented by SEQ ID NO: 965, and a reverse primer set represented by SEQ ID NO: 966, SK13_107 A set of the first allele-specific forward primer represented by SEQ ID NO: 967, the second allele-specific forward primer represented by SEQ ID NO: 968, and the reverse primer set represented by SEQ ID NO: 969, the first allele-specific forward primer represented by SEQ ID NO: 970 for SK13_113 1 allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 971 and the reverse primer set represented by SEQ ID NO: 972, the first allele-specific forward primer represented by SEQ ID NO: 973 for SK13_123, sequence The second allele-specific forward primer represented by No. 974 and the reverse primer set represented by SEQ ID NO: 975, the first allele-specific forward primer represented by SEQ ID NO: 976 for SK13_127, the second allele represented by SEQ ID NO: 977 A trait-specific forward primer and a set of reverse primers represented by SEQ ID NO: 978, a first allele-specific forward primer represented by SEQ ID NO: 979 for SK13_131, a second allele-specific forward primer represented by SEQ ID NO: 980, and SEQ ID NO: 981 A reverse primer set represented by, a first allele-specific forward primer represented by SEQ ID NO: 982 for SK13_138, a second allele-specific forward primer represented by SEQ ID NO: 983, and a reverse primer set represented by SEQ ID NO: 984, A first allele-specific forward primer represented by SEQ ID NO: 985 for SK13_150, a second allele-specific forward primer represented by SEQ ID NO: 986 and a reverse primer set represented by SEQ ID NO: 987, SEQ ID NO: 988 for SK13_157 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 989, and the reverse primer set represented by SEQ ID NO: 990, the first allele-specific forward primer represented by SEQ ID NO: 991 for SK13_164 A primer set for identifying sesame varieties, comprising 19 or more primer sets selected from the group consisting of a second allele-specific forward primer represented by SEQ ID NO: 992 and a reverse primer set represented by SEQ ID NO: 993.
The method of claim 3, wherein the 19 primer sets are a first allele-specific forward primer represented by SEQ ID NO: 61, a second allele-specific forward primer represented by SEQ ID NO: 62, and a reverse primer represented by SEQ ID NO: 63 for SK01_160. The primer set, the first allele-specific forward primer represented by SEQ ID NO: 85 for SK02_022, the second allele-specific forward primer represented by SEQ ID NO: 86, and the reverse primer set represented by SEQ ID NO: 87, for SK02-036 The first allele-specific forward primer represented by SEQ ID NO: 91, the second allele-specific forward primer represented by SEQ ID NO: 92, and the reverse primer set represented by SEQ ID NO: 93, the first set represented by SEQ ID NO: 247 for SK03_252 Allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 248 and the set of reverse primers represented by SEQ ID NO: 249, the first allele-specific forward primer represented by SEQ ID NO: 307 for SK04_158, SEQ ID NO: The second allele-specific forward primer represented by SEQ ID NO: 308 and the reverse primer set represented by SEQ ID NO: 309, the first allele-specific forward primer represented by SEQ ID NO: 328 for SK04_200, the second allele represented by SEQ ID NO: 329 A specific forward primer and a set of reverse primers represented by SEQ ID NO: 330, a first allele-specific forward primer represented by SEQ ID NO: 355 for SK05_059, a second allele-specific forward primer represented by SEQ ID NO: 356 and SEQ ID NO: 357 The reverse primer set represented by, the first allele-specific forward primer represented by SEQ ID NO: 403 for SK06_013, the second allele-specific forward primer represented by SEQ ID NO: 404, and the reverse primer set represented by SEQ ID NO: 405, SK06_162 The first allele-specific forward primer represented by SEQ ID NO: 466, the second allele-specific forward primer represented by SEQ ID NO: 467, and the reverse primer set represented by SEQ ID NO: 468, SEQ ID NO: 526 for SK07_073 The first allele-specific forward primer, the second allele-specific forward primer represented by SEQ ID NO: 527 and the reverse primer set represented by SEQ ID NO: 528, the first allele-specific forward primer represented by SEQ ID NO: 583 for SK08_039, The second allele-specific forward primer represented by SEQ ID NO: 584 and the reverse primer set represented by SEQ ID NO: 585, the first allele-specific forward primer represented by SEQ ID NO: 718 for SK09_186, the second allele-specific forward primer represented by SEQ ID NO: 719 Allele-specific forward primer and reverse primer set represented by SEQ ID NO: 720, first allele-specific forward primer represented by SEQ ID NO: 766 for SK10_061, second allele-specific forward primer represented by SEQ ID NO: 767 and SEQ ID NO: A reverse primer set represented by 768, a first allele-specific forward primer represented by SEQ ID NO: 769 for SK10_076, a second allele-specific forward primer represented by SEQ ID NO: 770, and a reverse primer set represented by SEQ ID NO: 771 , the first allele-specific forward primer represented by SEQ ID NO: 814 for SK11_001, the second allele-specific forward primer represented by SEQ ID NO: 815 and the reverse primer set represented by SEQ ID NO: 816, SEQ ID NO: 832 for SK11_042. The first allele-specific forward primer represented by SEQ ID NO: 833, the second allele-specific forward primer represented by SEQ ID NO: 834, and the set of reverse primers represented by SEQ ID NO: 834, the first allele-specific forward primer represented by SEQ ID NO: 901 for SK12_096 Primer, the second allele-specific forward primer represented by SEQ ID NO: 902 and the reverse primer set represented by SEQ ID NO: 903, the first allele-specific forward primer represented by SEQ ID NO: 922 for SK12_147, SEQ ID NO: 923 A second allele-specific forward primer and a set of reverse primers represented by SEQ ID NO: 924, a first allele-specific forward primer represented by SEQ ID NO: 961 for SK13_094, a second allele-specific forward primer represented by SEQ ID NO: 962, and A primer set for identifying sesame varieties, which is a reverse primer set represented by SEQ ID NO: 963.
A composition for identifying sesame varieties comprising the primer set of claim 3 or claim 4.
A kit for identifying sesame varieties comprising the composition of claim 5.
(a) isolating genomic DNA from sesame seeds;
(b) performing KASP using the primer set of claim 3 or claim 4 using the genomic DNA of step (a) as a template; and
(c) a method for determining a sesame variety using genomic DNA isolated from a sesame sample and the primer set, comprising the step of analyzing the amplification product of the KASP.
Genotype information profiled on 46 domestic varieties of sesame seeds using the primers of claim 3 or 4.
According to claim 8, the 46 varieties are Gilmi, Gangbaek, Gangan, Gangheuk, Geonbaek, Geonheuk, Gopum, Geumok, Nambaek, Namsan, Naman, Nuri, Dasak, Daheuk, Dubeol, Mangeum, Manheuk, Miheuk, Milseong, Sangbaek, Suwon, Sunheuk, Annam, Ansan, Yangheuk, Osan, Yumi, Yuseong, Yunheuk, Jungmo 5002, Jungmo 5003, Jungmo 5005, Jingi, Jinbaek, Jinyul, Jinju, Chamhwang, Pyongan, Pungnam, Poongsan, Pungan, Hansan, Handsome, Hwaheuk , The genotype information including yellow and white and black lines.

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