KR20230087771A - Novel bacteriophage specific for fire blight and use thereof - Google Patents

Abstract

The present specification relates to a bacteriophage with accession number KACC 97040P, which shows a specific lytic activity against Erwinia amylovora, has a control effect on fruit burn disease, and is a microorganism for controlling fruit burn disease Since it can be used as a pesticide and can be used instead of antibiotics, it prevents the emergence of antibiotic-resistant strains and does not affect the environment, so safe and effective control of water blight can be expected.

Description

Novel bacteriophage specific for fire blight and use thereof {Novel bacteriophage specific for fire blight and use thereof}

The present specification relates to a bacteriophage having a strong lytic activity specific to fruit burn blight, an agrochemical composition containing the bacteriophage, and a method for controlling burn disease using the same.

Fruit tree fire blight (fire blight) is one of the representative bacterial plant quarantine diseases, and Erwinia amylovora is known as the causative agent. Fruit burn blight causes the flowers, branches, and fruits of pears or apple trees to turn black, showing symptoms of drying out black as if burned. Erwinia amyloba is transmitted by insects or wind and rain and invades the host through flowers, nectar glands, stomata, skins, and wounds, so it is highly contagious and can invade about 70 species of plants such as pear trees, apple trees, and apricot trees. Due to the characteristic of being there, the damage caused by fire blight is occurring worldwide.

Because fruit tree burn disease spreads quickly, even if symptoms appear on only one tree, the entire orchard must be closed, and fruit trees within a radius of 100 m must be uprooted, burned, and disposed of by burying them in the ground. Because of this, fruit burn blight not only ruins the current harvest, but can also cause long-term damage to farms.

As a response to fruit tree burn disease, by reducing soil moisture and maintaining the balance of fertilizer nutrients, the infection and spread of burn disease can be controlled, and by removing infected fruit trees or blackened branches in winter when pathogens have a dormant period. Methods for treating burns are known, but the occurrence of essential diseases cannot be eliminated.

The present inventors studied phage therapy, which is a control (treatment) method using bacteriophage as a control method for Erwinia amylobora, and as a result, the new bacteriophage isolated from the water of a pear orchard showed strong lytic activity against Erwinia amylobora. found

The present specification is to provide a bacteriophage having a specific and strong lytic activity against Erwinia amylovora .

In addition, the present specification is to provide an agrochemical composition containing the bacteriophage, specifically an agrochemical composition for controlling fruit burn blight.

In addition, the present specification is to provide a burn disease control method using the bacteriophage.

The present specification provides a bacteriophage having a lytic activity specific to Erwinia amylovora, Accession No. KACC 97040P.

The bacteriophage of the present invention is characterized by having the nucleotide sequence of SEQ ID NO: 1.

The bacteriophage of the present invention is characterized in that it has activity at 20 ℃ to 60 ℃.

The bacteriophage of the present invention is characterized by having activity at pH 3 to 12.

According to another embodiment, the present specification provides an agrochemical composition comprising the bacteriophage as an active ingredient.

The bacteriophage contained in the agrochemical composition of the present invention is characterized in that it is used for preventing or treating fruit tree burn (fire blight).

According to another embodiment, the present specification provides an antibiotic composition containing the bacteriophage as an active ingredient. According to another embodiment, the present specification provides a disinfectant containing the bacteriophage as an active ingredient.

According to another embodiment, the present specification provides a cleaning agent containing the bacteriophage as an active ingredient. According to another embodiment, the present specification provides a method for controlling fruit burn disease using the bacteriophage.

According to another embodiment, the present specification provides a use of the bacteriophage for use in preventing or treating fire blight.

According to another embodiment, the present specification provides a use of the bacteriophage for use in the manufacture of an agrochemical composition for preventing or treating fruit tree burns (fire blight).

The bacteriophage, accession number KACC 97040P, newly isolated in the present invention has a specific lytic activity (killing ability) against Erwinia amylovora. It is specific only to Erwinia amylovora (fruit burn bacillus) and has no lytic activity in other Erwinia species (e.g. Erwinia pyrifoliae), so the specificity for fruit burn bacillus is very high, and it is effective against all strains within the same Erwinia amylovora species. Not strain-dependent. In addition, it has a high stability against temperature change or pH change, and thus has an excellent effect as an environmentally friendly burn disease control agent.

Figure 1 shows the formation of plaques by the bacteriophage of the present invention in an experiment performed using Erwinia amylobora as a host.
Figure 2 confirms the lytic activity of the bacteriophage of the present invention against Erwinia amylobora.
Figure 3 confirms the bacteriophage of the present invention with a transmission electron microscope.
Figure 4 relates to the lytic activity of the bacteriophage of the present invention according to the temperature.
Figure 5 relates to the lytic activity of the bacteriophage of the present invention according to pH.
Figure 6 confirms the effect of controlling fruit burn blight in Yugwa.

Hereinafter, the present specification will be described in more detail. Erwinia amylovora (Erwinia amylovora) provides a bacteriophage having a specific lytic activity, accession number KACC 97040P.

The present inventors selected a novel bacteriophage with excellent lytic effect and host specificity for Erwinia amylobora, named it Erwinia phage Fifi451 (hereinafter referred to as Fifi451), and deposited with the National Academy of Agricultural Sciences on July 27, 2021 and was granted KACC 97040P.

The present inventors isolated the bacteriophage Fifi451 from the water of a pear orchard in Seoun-myeon, Anseong, Gyeonggi-do, and in an experiment conducted using Erwinia amylovora strain YKB14808 as a host, it was confirmed that plaque was formed to lyse Erwinia amylovora (Fig. One).

Referring to Figure 2, it can be seen that even when the multiplicity of infection (MOI) level of bacteriophage Fifi451 is low, the growth of host cells can be inhibited (control in Figure 2 is the case where bacteriophage Fifi451 is not injected).

After pure separation of bacteriophage Fifi451 by CsCl density gradient ultra-high-speed centrifugation, it is shown in FIG. 3 that the shape of the phage was confirmed with an electron microscope. It was found to belong to the Myoviridae family, which consists of a head of about 65 nm and a tail of about 124 nm.

The bacteriophage Fifi451 of the present invention is effective against all strains within the Erwinia amylovora species. Erwinia pyrifolia ( Erwinia pyrifoliae ), Dikeya zea ( Dickeya zeae ), Entirobacter cloacae ( Enterobacter cloacae ), Escherichia coli ( Escherichia coli ), Pantoea agglomerans ( Pantoea agglomerans ) does not show lytic activity .

In addition, since the bacteriophage of the present invention does not infect other hosts other than bacteria such as plants, it has the advantage of solving the problem of antibiotic-resistant bacteria caused by misuse and abuse of antibiotics, the problem of residual antibiotics in food, and the problem of a wide range of hosts.

Therefore, the bacteriophage of the present invention can be used in various fields such as prevention or treatment of infectious diseases caused by Erwinia amylobora bacteria, antibiotic compositions, feed additive compositions, feeds, disinfectants, or detergents.

Bacteriophage Fifi451 of the present invention has the nucleotide sequence of SEQ ID NO: 1. Specifically, bacteriophage Fifi451 has a base sequence of 53,219 bp.

Bacteriophage Fifi451 may have activity at 15 °C to 70 °C, 20 °C to 60 °C, 25 °C to 60 °C, 25 °C to 55 °C, and 25 °C to 50 °C. Specifically, even after treatment at 50 ° C. for 1 hour, lytic activity was shown against Erwinia amylobora.

Bacteriophage Fifi451 may have activity at pH 1 to 15, pH 2 to 14, pH 3 to 13, pH 3 to 12, pH 4 to 12, and pH 4 to 11. It shows lytic activity against Erwinia amylobora even when exposed to various pH.

According to another embodiment, the present invention provides an agrochemical composition comprising the bacteriophage as an active ingredient.

The present invention can provide a composition with excellent proliferation inhibitory and killing effects on all strains within the species of Erwinia amylovora, and this composition contains bacteriophage Fifi451 as an active ingredient.

The bacteriophage of the agrochemical composition is used for the prevention or treatment of fruit tree burn (fire blight).

The agrochemical composition according to the present invention described above may be used for fruits that may cause fruit burns, for example, apples, pears, etc., but is not limited thereto. effective in controlling fruit tree fire blight.

On the other hand, any one selected from the pesticide composition of the present invention, the composition for controlling fruit burn blight, and the composition for inhibiting or killing Erwinia amylovora growth is a kind of microbial pesticide, which is available in various forms such as liquid and powder. can be manufactured.

In addition, if necessary, various known excipients used in microbial pesticides may be added. Since the excipients may be used without being limited to specific ones used in microbial pesticides, description of the excipients will be omitted. For example, the pesticide composition according to the present invention may use surfactants or extenders that can be used as biochemical pesticides as excipients for the purpose of stable formulation suitable for use in actual packaging. In this case, the agricultural chemical composition may be prepared in one or more forms selected from the group consisting of a hydration agent, a granular hydration agent, a liquid hydration agent, a liquid agent, a water-soluble agent, a water-soluble granule agent, and an encapsulating agent. An example of the surfactant is an alkyl (C ₈ ~C ₁₂ ) Arylsulfonate, dialkyl(C ₃ ~C ₆ )arylsulfonate, dialkyl(C ₈ ~C ₁₂ )sulfosuccinate, lignin sulfonate, naphthalenesulfonate condensate, naphthalenesulfonate formalin condensate, Sodium or calcium salts of sulfonate compounds such as alkyl (C ₈ ~C ₁₂ )naphthalenesulfonate formalin condensate, polyoxyethylenealkyl (C ₈ ~C ₁₂ )phenylsulfonate, alkyl (C ₈ ~C ₁₂ )sulfate Sodium or calcium salts of sulfate compounds such as alkyl (C ₈ ~ C ₁₂ ) aryl sulfate, polyoxyethylene alkyl (C ₈ ~ C ₁₂ ) sulfate, polyoxyethylene alkyl (C ₈ ~ C ₁₂ ) phenyl sulfate, poly Sodium or calcium salts of succinate compounds such as oxyalkylene succinate, sodium benzoate, anionic surfactants such as alkyl carboxylates, polyoxyethylene alkyl (C ₈ ~ C ₁₂ ) ether, polyoxyethylene alkyl ( C ₈ ~ C ₁₂ ) phenyl ether, polyoxyethylene alkyl (C ₈ ~ C ₁₂ ) phenyl polymer, selected from the group consisting of nonionic surfactants such as ethylene oxide propylene oxide copolymers, polycarboxylates, Triton 100 and Tween 80 One or two or more may be used in combination, and it will be easily understood by those skilled in the art that other surfactants other than these may be used.

Extenders include bentonite, talc, clay, kaolin, calcium carbonate, silica sand, pumice, diatomaceous earth, acid clay, zeolite, perlite, white carbon, ammonium sulfate, urea, glucose, dextrin, soy flour, rice, wheat , Loess, glucose and starch, water, etc. may be used alone or in combination of two or more, and it may be easily understood by those skilled in the art that other extenders other than these may be used. will be.

The present invention provides an antibiotic composition comprising the bacteriophage as an active ingredient.

The present invention provides a composition for preventing or treating diseases caused by Erwinia amylovora, comprising the bacteriophage as an active ingredient.

The antibiotic composition, preventive or therapeutic composition containing the bacteriophage as an active ingredient is safe because it does not induce drug resistance or resistance compared to existing antibacterial substances or antibiotics, and has high specificity for Erwinia amylobora.

The present invention provides a disinfectant comprising the bacteriophage as an active ingredient.

The present invention provides a detergent containing the bacteriophage as an active ingredient.

The disinfectant or cleaner containing the bacteriophage as an active ingredient has excellent disinfection or cleaning effect and has high specificity for Erwinia amylobora.

The present invention provides a method for controlling fruit burn disease using bacteriophage Fifi451. The fruit tree burn is caused by Erwinia amylovora, and the agrochemical composition containing the bacteriophage as an active ingredient can be treated with the bacteria to kill Erwinia amylovora.

Phage therapy, which is a control (treatment) method using bacteriophage according to the present invention, prevents the emergence of antibiotic-resistant strains and does not affect the environment, so safe and effective control of fruit burn disease can be expected.

The present invention provides a use of the bacteriophage for preventing or treating fruit tree fire blight.

The present invention provides a use of the bacteriophage for use in the manufacture of an agrochemical composition for the prevention or treatment of fruit tree burn (fire blight).

Specific information on the bacteriophage, fruit burn disease, prevention, treatment, pesticide composition, etc. may be the same as described above or applied mutatis mutandis.

It should be understood that the terms used herein are only for describing specific embodiments and are not intended to limit the scope of the present invention, which is limited only by the appended claims. All technical and scientific terms used herein have the same meaning as commonly understood by a person of ordinary skill in the art unless otherwise specified.

In this specification, when a part is said to "include" a certain component, it means that it may further include other components without excluding other components unless otherwise stated.

On the other hand, various embodiments and embodiments of the present invention can be combined with any other embodiments unless clearly indicated to the contrary. Any feature indicated as being particularly desirable or advantageous may be combined with any other features and characteristics indicated as being particularly desirable or advantageous.

In the present specification, "bacteriophage" is a virus that infects a specific target bacterium. The head of a bacteriophage, which is enclosed in a protein capsid, consists of a nucleic acid genome, and some bacteriophages have a tail composed of lipids or proteins. Most bacteriophages contain double-stranded DNA as their genetic material.

"Pesticide" in the present specification means a drug used for the protection and growth of crops, chemical pesticides and biological pesticides used to control pests, pests, weeds, etc. that occur during cultivation or storage of crops, and physiological functions of crops It is a generic term for growth regulators used to promote or inhibit growth, supplements that enhance drug efficacy, etc.

In the present specification, "control" means to prevent and relieve various diseases and pests that damage crops.

In the present specification, "antibiotic composition" refers to an agent that is provided to animals and plants in the form of a drug to kill bacteria, and refers to preservatives, bactericides, antibiotics, and antibacterial agents.

"Antibacterial agent" in the present specification means a substance that kills or inhibits the growth of microorganisms, and includes preservatives, bactericides, and antibiotics.

"Disinfectant" in the present specification can be usefully used as a hospital and health disinfectant to prevent hospital infection, and can be used as a general life disinfectant, disinfectant for food and cooking places and facilities, buildings such as orchards, livestock, drinking water, litter, egg beds It can be used for disinfection of various growing products such as transport vehicles and tableware.

"Detergent" in this specification means a drug used for the purpose of cleaning.

Hereinafter, examples and comparative examples will be described in detail to describe the present specification in detail. However, the Examples and Comparative Examples according to the present specification may be modified in many different forms, and the scope of the present specification is not construed as being limited to the Examples and Comparative Examples detailed below. Examples and comparative examples in the present specification are provided to more completely explain the present specification to those skilled in the art.

[Example 1] Separation of bacteriophage

1) Environmental sample pretreatment for bacteriophage isolation

To isolate bacteriophages, we collected soil and water from an orchard (pear orchard in Seoun-myeon, Anseong, Gyeonggi-do) where fruit tree burns occurred and nearby. 10 g for soil samples and 10 ml for water samples are placed in a 50 ml conical tube and 10 ml of TSB (tryptic soy broth) is added. To this, 1 ml of a mixed culture medium in which 94 strains of Erwinia amylovora were shaken and cultured for 24 hours was added to each of the mixed cultures and cultured with shaking for 24 hours. The incubation temperature is 27°C. The cultured sample is centrifuged at 10,000 × g for 5 minutes, and only the supernatant is transferred to a new tube. The sample thus obtained was filtered through a 0.2 μm pore size filter to completely remove bacteria.

2) Dotting assay

A dotting assay was performed to confirm the presence of bacteriophage in various environmental samples that had been subjected to pretreatment for inducing proliferation of bacteriophage. A medium sterilized by adding 0.4% soft agar to TSB was prepared at 40-50 ° C. Add 15 μl of one type of fruit burn bacillus cultured with shaking overnight to 3 ml of medium, mix to avoid bubbles, pour thinly into TSA (tryptic soy agar), and harden for 10 minutes. After dropping 10 μl of the filtered environmental sample on the medium uniformly containing the strain, it is cultured at 27°C. When several environmental samples are dropped together into one medium, sufficient spacing must be maintained so that the samples do not mix. After 24 hours of incubation, the sample was judged positive if bacteria did not grow and if a clear or plaque was confirmed.

3) Plaque assay (overlay assay)

An overlay assay was performed to isolate pure bacteriophages. Prepare a sterilized medium at 40~50℃ by adding 0.4% soft agar to TSB. In 3 ml of medium, add 15 μl of fruit burn bacilli cultured with shaking overnight and 10 μl of a positive sample confirmed by bacteriophage, mix so that no bubbles are formed, pour thinly into TSA, harden for 10 minutes, and incubate at 27 ° C for 24 hours. At this time, the positive sample should be performed by concentration by decimal dilution to obtain a single plaque. After culturing for 24 hours, plaques of different sizes or shapes were selected, put in 1 ml of saline-magnesium (SM) buffer, and stored in a refrigerator. Bacteriophages extracted from each plaque were repeated 5 or more times to isolate a single bacteriophage. A plaque formed at this time is shown in FIG. 1 .

[Example 2] Confirmation of lytic activity

Fruit burn blight ( Erwinia amylovora YKB14808) was prepared by shaking culture in TSB medium the day before, and then cultured 24 hours later. Then, when the concentration of bacteria reaches OD _600nm =0.5 (10 ⁸ CFU/ml), the inoculation concentration is adjusted to 10 ⁵ CFU/ml by diluting in decimal in TSB medium. After dispensing 180 μl of the bacterial culture medium into a 96-well plate, phages are inoculated in 20 μl each according to multiplicity of infection (MOI) 0.001, 0.1, 10, 1000. 2 shows that the OD value of the bacteria was measured every hour while shaking culture at 27° C. and 120 rpm in a microplate reader for 24 hours.

[Example 3] Morphological analysis

For the proliferation of bacteriophage, the burn bacillus ( Erwinia amylovora YKB14808) is cultured in 3 ml of TSB with shaking for 24 hours. After 2 ml of shaking culture for 24 hours was re-cultured in 200 ml of TSB, and when OD ₆₀₀ =0.5, 1 ml of phage at a concentration of 10 ⁸ PFU/ml was treated, followed by shaking culture for 24 hours. In order to remove impurities from the shake-cultured sample for 24 hours, centrifugation was performed at 10,000 × g for 10 minutes, and the supernatant was filtered through a 0.22 μm pore size filter. The bacteriophage sample was treated with 5 g of Ployethylene glycol 8000 (PEG 8000) and 10 ml of 5 M NaCl, and then incubated at 4 ° C for 24 hours. The sample cultured for 24 hours is centrifuged at 9,000g for 20 minutes, the supernatant is removed, and the precipitate is suspended in SM buffer to prepare. After stacking 3 ml of CsCl density layers (1.3 g/ml, 1.45 g/ml, 1.5 g/ml, 1.7 g/ml) in reverse order in an ultra-high-speed centrifugal separation tube, the concentrated phage samples were stacked on top of them, and at 785,500 × g Purified phages were obtained by centrifugation for 2 hours. The purified phage was dialyzed with SM buffer and then filtered through a 0.22 μm pore size filter. After staining the filtered phage sample with a 2% uranyl acetate solution, the morphology was confirmed through a transmission electron microscope and shown in FIG. 3 .

[Example 4] Host range analysis

For the host range analysis of the isolated bacteriophages, Erwinia amylovora 94 strains, Erwinia pyrifoliae 25 strains , Bacillus thuringiensis, Dickeya zeae, Enterococcus faecalis, Escherichia coli, Pantoea agglomerans, Pectobacterium carotovorum, Pseudomonas fluorescens, Staphylococcus xylosus, and Xanthomonas campestris were used. . As a positive control, AgriPhage, a bacteriophage preparation for controlling fire blight, was used, and SM buffer was used as a negative control. The dotting assay method was used for host range analysis, and when plaques were confirmed after 24 hours of culture, it was judged positive. Specifically, the case where a transparent ring was generated was marked as +, and the case where it was not was marked as -, and the results are shown in Table 1 below. All experiments were repeated 3 to 9 times, and the average value of the stage scores of observed plaques was recorded.

No. target bacteria strain Lytic activity of bacteriophage Fifi451 One Erwinia amylovora YKB 14715 + 2 Erwinia amylovora YKB 14740 + 3 Erwinia amylovora YKB 14742 + 4 Erwinia amylovora YKB 14748 + 5 Erwinia amylovora YKB 14750 + 6 Erwinia amylovora YKB 14754 + 7 Erwinia amylovora YKB 14756 + 8 Erwinia amylovora YKB 14758 + 9 Erwinia amylovora YKB 14768 + 10 Erwinia amylovora YKB 14770 + 11 Erwinia amylovora YKB 14776 + 12 Erwinia amylovora YKB 14778 + 13 Erwinia amylovora YKB 14787 + 14 Erwinia amylovora YKB 14806 + 15 Erwinia amylovora YKB 14808 + 16 Erwinia amylovora YKB 14814 + 17 Erwinia amylovora YKB 14818 + 18 Erwinia amylovora YKB 14820 + 19 Erwinia amylovora YKB 14822 + 20 Erwinia amylovora TS3128 + 21 Erwinia amylovora RA0023 + 22 Erwinia amylovora RA0024 + 23 Erwinia amylovora RA0025 + 24 Erwinia amylovora RA0026 + 25 Erwinia amylovora RA0027 + 26 Erwinia amylovora RA0028 + 27 Erwinia amylovora RA0029 + 28 Erwinia amylovora RA0030 + 29 Erwinia amylovora RA0031 + 30 Erwinia amylovora RA0032 + 31 Erwinia amylovora RA0033 + 32 Erwinia amylovora RA0034 + 33 Erwinia amylovora RA0035 + 34 Erwinia amylovora RA0036 + 35 Erwinia amylovora RA0037 + 36 Erwinia amylovora RA0038 + 37 Erwinia amylovora RA0039 + 38 Erwinia amylovora RA0040 + 39 Erwinia amylovora RA0041 + 40 Erwinia amylovora RA0042 + 41 Erwinia amylovora RA0043 + 42 Erwinia amylovora RA0044 + 43 Erwinia amylovora RA0045 + 44 Erwinia amylovora RA0046 + 45 Erwinia amylovora RA0047 + 46 Erwinia amylovora RA0048 + 47 Erwinia amylovora RA0049 + 48 Erwinia amylovora RA0050 + 49 Erwinia amylovora RA0051 + 50 Erwinia amylovora RA0052 + 51 Erwinia amylovora RA0053 + 52 Erwinia amylovora RA0054 + 53 Erwinia amylovora RA0055 + 54 Erwinia amylovora RA0056 + 55 Erwinia amylovora RA0057 + 56 Erwinia amylovora RA0058 + 57 Erwinia amylovora RA0059 + 58 Erwinia amylovora RA0060 + 59 Erwinia amylovora RA0061 + 60 Erwinia amylovora RA0062 + 61 Erwinia amylovora RA0063 + 62 Erwinia amylovora RA0064 + 63 Erwinia amylovora RA0065 + 64 Erwinia amylovora RA0066 + 65 Erwinia amylovora RA0067 + 66 Erwinia amylovora RA0068 + 67 Erwinia amylovora RA0069 + 68 Erwinia amylovora RA0070 + 69 Erwinia amylovora RA0071 + 70 Erwinia amylovora RA0072 + 71 Erwinia amylovora RA0073 + 72 Erwinia amylovora RA0074 + 73 Erwinia amylovora RA0075 + 74 Erwinia amylovora RA0076 + 75 Erwinia amylovora RA0077 + 76 Erwinia amylovora RA0078 + 77 Erwinia amylovora RA0079 + 78 Erwinia amylovora RA0080 + 79 Erwinia amylovora RA0081 + 80 Erwinia amylovora RA0082 + 81 Erwinia amylovora RA0083 + 82 Erwinia amylovora RA0084 + 83 Erwinia amylovora RA0085 + 84 Erwinia amylovora RA0086 + 85 Erwinia amylovora RA0087 + 86 Erwinia amylovora RA0088 + 87 Erwinia amylovora RA0089 + 88 Erwinia amylovora RA0090 + 89 Erwinia amylovora RA0091 + 90 Erwinia amylovora RA0092 + 91 Erwinia amylovora RA0093 + 92 Erwinia amylovora RA0094 + 93 Erwinia amylovora RA0095 + 94 Erwinia amylovora RA0096 + 95 Erwinia pyrifoliae Ep12327 - 96 Erwinia pyrifoliae RP0098 - 97 Erwinia pyrifoliae RP0099 - 98 Erwinia pyrifoliae RP0100 - 99 Erwinia pyrifoliae RP0101 - 100 Erwinia pyrifoliae RP0102 - 101 Erwinia pyrifoliae RP0103 - 102 Erwinia pyrifoliae RP0104 - 103 Erwinia pyrifoliae RP0105 - 104 Erwinia pyrifoliae RP0106 - 105 Erwinia pyrifoliae RP0107 - 106 Erwinia pyrifoliae RP0108 - 107 Erwinia pyrifoliae RP0109 - 108 Erwinia pyrifoliae RP0110 - 109 Erwinia pyrifoliae RP0111 - 110 Erwinia pyrifoliae RP0112 - 111 Erwinia pyrifoliae RP0113 - 112 Erwinia pyrifoliae RP0114 - 113 Erwinia pyrifoliae RP0115 - 114 Erwinia pyrifoliae RP0116 - 115 Erwinia pyrifoliae RP0117 - 116 Erwinia pyrifoliae RP0118 - 117 Erwinia pyrifoliae RP0119 - 118 Erwinia pyrifoliae RP0120 - 119 Erwinia pyrifoliae RP0121 - 120 Dickeya Zeae - 121 Enterobacter cloacce - 123 Escherichia coli - 124 Pantoea agglomerans - 125 Pectobacterium carotovorum - 126 Pseudomonas fluoresceus - 127 Xanthomonas campestris - 128 Bacillus thuringieusis - 129 Clavibacter michiganensis - 130 Enterococcus faecalis -

Referring to Table 1, it can be seen that the bacteriophage Fifi451 is not strain-dependent because it has high specificity for Erwinia amylovora and is effective against all strains within the species Erwinia amylovora.

[Example 5] Temperature and pH stability

Stability was analyzed to determine whether the activity was maintained at various temperatures and pHs of the bacteriophages. The concentration of each phage was 10 ⁵ PFU/ml. 200 μl of each phage was dispensed into a 1.5 ml tube, treated at 30 ° C, 40 ° C, 50 ° C, and 60 ° C for 1 hour, and the change in active concentration was confirmed through dotting assay, as shown in FIG.

The pH was prepared according to pH 4 to 11 using HCl and NaOH in SM buffer. After treating 10 μl of phage at a concentration of 10 ⁷ PFU/ml in 990 μl of SM buffer prepared for each pH for 1 hour, the change in active concentration was confirmed through a dotting assay and is shown in FIG. 5 .

It can be seen that the bacteriophage Fifi451 of the present invention has excellent effects as an environmentally friendly burn disease control agent due to its high stability even in temperature or pH changes.

[Example 6] Base sequence analysis

The bacteriophage particles were treated with protease to remove the outer shell, and genomic DNA (gDNA) of each phage was extracted using Norgen's phage DNA isolation kit. After preparing a library of 100-200 ng of gDNA using the TruSeq Nano DNA kit, next generation sequencing (NGS) was performed based on the Illumina HiSeqXTen platform. All reads obtained were assembled to obtain a single contig. The function of each open reading frame predicted by sequence analysis was analyzed using Basic Local Alignment Search Tool (BLAST) and HHpred. The genome of Erwinia phage Fifi451 has a size of 53,219 bp and 77 open reading frames (ORFs) were predicted.

[Example 7] Confirmation of effect of inhibiting fruit tree burns

Fruit burn bacillus ( Erwinia amylovora YKB14808) was prepared by shaking culture in TSB medium the day before. When the bacterial culture medium is re-cultured in TSB medium and reaches OD _600nm =0.5 (10 ⁸ CFU/ml), centrifugation at 1,800×g for 5 minutes precipitates burn bacilli, washed with Saline solution, and diluted 10 times to obtain a bacterial suspension. prepare Immature fruits used in the experiment are surface sterilized with 70% ethanol. Using a sterilized 1 mL tip, make two holes on the surface of the immature fruit. Inoculate 10 μl of 10 ¹⁰ PFU/ml phage solution into the hole made on the surface of the immature fruit without flowing. Thereafter, before the phage solution completely dries, 10 μl of 10 ⁷ CFU/ml bacterial suspension was inoculated, the relative humidity was maintained at 90% or more, and the degree of disease occurrence was confirmed at 27 ° C for about 2 weeks at 2-day intervals (Fig. 6). showed up Referring to FIG. 6, it can be seen that fruit burn blight is controlled in the milkweed inoculated with bacteriophage Fifi451.

[base sequence]

SEQ ID NO: 1

AGCTAGATGAAGTACAGCTGACCGACCATCCATCGCATATCCCATTACAAATATCACATG

CATAATATTGTAGCAATATTTGGAATTGAATATTGTTAACTTATTTCTTTCTTAATAGTC

TCGGAAAGGATGTTCGCTTTCGCGACCACCTTCCCTCAACATCTAACTGACAAGATTCAA

TGCACCTTAATTTCGGCATCTTCGTCGATAATCTTGAGCAAGGCTTCCTGCATTTGAATC

AACTTTTCAGGAGTCCAGATTGATTTATCAAGGAAGATAATAGTGCAATGGTGTTCTTCG

GCAAGATTTGCCAAGTATTGCATTAGAGCTTTATCCATTATCCGTTACCTAAATATTGAT

AAAATTTGTGATTTCCGACCTGTTTTATGAACAAAGTACGTCCAAATTTGTCAGATTTTG

ATGAAAAATGAGTACATTTTCCTGTGTTTTTCGTGTCTAAAATCACAAATTTCAATGCAA

TTTCAAGACTTTCTACCACAGCTTTGTGCTCTTTTGAGTCAAATTTCCGCGCTACTTCTC

TAAAAGAAGTGTTCCGCTTCCATTTGTTTGAAGTCCATGAAAACTGGCGTGGCTGATAAA

TTACACCACAGATGGTGTTTGAAAATGGCTTACCAAACTTAGCTCTGTTCAGTACGACAT

TTGCAACCATGACCTTTCCAGCATAGCTTTCTCCTCTGGCTTCGAAGAAGATAGCTTTAG

CCAAACAGTCAACTTCATGTTTCTGACTTGCAGTTAATTTCAATCCACCTTTGGAGATAAG

ATTGAGCTGCAAACTCCGACACATGCGCATTCCATTTGGAATTCACTCCAGCATCAGCAG

TTGCCAATGCCGAAGTGTTCCACGCAAATATCAGGAGCATGATGCAAAACAACTTCCGCA

TCTGTGTTCCTTTTATCTTGCTTTTCTGAACCAAGTTAATGGATTCAATTGCTTAATTGC

TGCCGAGAACGAATCACGTAAAGTAGTGATAATTAACTTCGCACCTACTACACCAATCAC

AATGCAAACAACAAATCCTGCATTAGGATGATTAACCAAATAACCATCCAGAATTGGGAG

TGAGAAGACAAACGTTCCAATAACACAACACAGCGCTGCATCAAGCAGAGCTTTTTCCAAG

ACCTTCACTCTCATAGGCTCCACGAATTAAAGCAGTAAGTCCAGCTAACAGACCATGCAG

TAATCCATACTGTTGCATGAATAGGAAGATTACTTGGATAGTTTGTCTATCCACTTTGTA

GCCCTTATCAATCACCATATCGCATACACGTTAACGTTGACCGCGACATTCTGTGGATTG

TAGATGTTACACCATTTTGAGAAATGCCGTTAAACCACGCCCTGTTCTCACCATTGAAA

GCTGAGAAGAATACAGCAGGACTTCCAGGGAATTGCGCCGGGAAATTCCAAGTTGTGGTT

GCATTTGCTGCAAGAGACAAGTTCTGTCTACACCACATAGGACCATTTGGAATCTTAGTC

CAAGCACCATTACCGTTTGTACCAGACTGGAATTGGTCTAGACGTACAGCATGTGCACCA

CTTGTTGCACCACCAACATCAAACTGTGTGTACTGGTTGCCATTTACAGCAGCCTTACTT

TGCAGCATGAAGTTGAGTCGAACATTGTTTACAGCATAGTTATCAGAACCACTGTTCGAA

CACTGGAATGCTTGACTTGCATCGCCGTTTATGTAGGCAGCATACAAACTACCCCTTGCA

ACAGTCCTGTCGGCAACATCAGTCAAGTTGTTGTTCTTCTGCAAAGCATTGACAATTCGT

GAGTCATTACCAGCAGCGACAGTGTTTGCAGTGGTGCCAACATCAAGAAATGCAGCACCT

TTTAACGCAGCTGTAAGTGCGCCGAAAGACCAACCTTTGGCCATTTTTAACCCTTATAGC

GTTGAGATTTGTTTTGCCGTCAAAGCACCAATCCAGAGTTTAAAGTTTTTGGCTTTAATC

AGTCTCTGGTTTGCTTGTGCCTGAACATCTGCACGGAGGAAGTTGTAATCACTTCCTGTG

ATGTTTGCATTGTTGGCTGCAACTGTACTAACCAGAACTCCGTTGAGATACAGCGACAAA

GAAGTTGTAGTTCTTACACCAACAAGACGAAGCCAGACACTGGTTGGAAGTGGGATTGTT

CCAGTTGTTCCAGTCTCAGACCAGAAACGAACTGTGTTATTCTTATTAACTTGAATAACA

CCACAACCAGCAGCAGCACCAAATGATGCATACAATTCAGGAGCAGTTGTAGTTCCGCCA

GTTCCTTCAAGGAAAACATCAACACAAATGGTTGCTTCTTTCAAACCTTCAAGAGGTTTG

TATGTATACACAACACGGTTGTCATATTGTGTATTGTTTCCAAAGATTACACCATTCAGT

AGAGATGGATTCCAATCCAGTGCACCACCATTTTGGTCGGCCTGAACAGTACCACCGCCA

ATGTAGACATTCAAATCAGATGCAAGAGGCAAATAGAACACAGGTTGCTGCACTGCATAG

GCTGCTTCAACAGCAGCTTTATTATCTGCAAATGTTGCTTTAGTAATGATTTTGGTTGCT

GGAGCATTCCCAGTTTTGACAAAACCTTCACCAGAAGCATCAACACCAACTTGACCTGCA

CTGTTCGCCATATAGACGCCAGTGTCATCAGCTTTGATTTTACGTACACCATCGGCAGAC

AGAAGAACTTGACCAGTCTTCATTTCAACATTGCCGGTGTATGCACCACCAGTTACAGGA

ACTGCGCCAATGTCAGTGGCTGTAACTCCATGTGGGTTATCTTTCCTTGCTGCATGGTCC

GAAACCATTTTCTTGTACTGAGCAACCAATGCATCAATTTGTGCAGTGGTGTATGTTCCA

AGTCTTCCCGGAGTCAGTCCATGAGGGTTACTTGTTACATCAGCGATGTGACTTGCGATT

GCCGCAGAAATTGCATCATAATCTGCACGAGTAATCTGCAATGCACTTGGAGTCCATTGA

GCCAAGTTTGAACTTGGTTTCAGCGTCTTGCTTGGACCATCAAGTTTGGCAATATAAATC

ACTCCATTTGATTCATCCCAAACCGCAGCACCATTTCGGTAGGTTGCATCGCCACCCCAT

TCAAACACACCACGTTCAGTAAGAGCTTGCAGTGTTGAATCCGTCTTCCATTGAAGATAG

TTCAGAACTTGGTATGTTGGGATTTCAGACAGCCAACCTTTTAAATATTTTTCGTCGCCT

GGGTCACGACGAGCAACCGAATTCGAGTCACCCCAAATTCGGCCAAGTTTAGGACGCAGC

ATTATCTATTCCTATACAGCAGTGATAGTTAAAATTGCAGCCATTCCGTTCCATTTAACA

GTTAAGGTTGCAGTTCCTGCTTTCAGGATGTTGATTACAGCCTGACCATTTGCTTGTGTT

GAACCAACAATCTGAACAACACTTGAATCGGAAGTTGTCCACACAGCACTGCCATCCAAT

CGAGCACCACGAACATCGTCAGGACTCCAACGGAATGCACCAATATACTGACCATTTTTG

GGAGCAGTTGCATTAGGCATTACGAAAACATCTGTCGTGACTTTGGAAACATACCAAGCT

GTTCCCGGAGTTGAAGATGTATCATTTGCAATATGCAAAGAATCATACTTCGGTTTCAGG

TTCAACTTCTGGTATTCAGTCAGTGCAAGATAAATATCCATAACACACAAATGCGTTGCA

TCAGGAATCAATGCTTGCAGGAATATTTTCAGATTTGGTTTGACGACAACTTGTGTGGCA

TCAACAACAGCAAACTTCCTGATATCGCCAGCATAGTATTCAAAGTAAATTGTTGCAGGA

ATACTAACTAATCCCACCCAAGTCATGAATGTATTATCGGCAATCTTCCATCCCTTATTC

CCTTCGAACACTGGAGCATCGGTTGTTTCAACAATGAAGTTACCGACACCCATTGTGTCA

AAAGAACCAGCACGAAGCTGCATTTGCAGAATCGGTTGAGGAAGTGCAAAGGAATTATTT

ACTGCAATTTGATGTGCATCGAAGTTCGCAACAGTTGTTATATCACTGCGAACACCAGAA

GACTCAACACCAACAGTTGTATCGCCATCACCTTCAAGGAATAGCGAACCACCGGAATTT

GTAATCTTCTGGTCTGGTCCATCAATTCGGAAACTTCCCGTACCTATTGTTAAACCACCA

ATGTAATTAACATCACCAGTAAAGGCTCCGCCACTGGTAGGCAAGGTACCAACTTGCGCT

GGAGTTTCGCCATGAACTTTACCTCCTGTCTGACTTTCGTGGTAAACGATTGTTGCAGGG

TTGTTCGGGTCGCCAAAGAATTTATCAATCTCACCAGACTCATATCCACCAATATCATGG

ATGTTATCTTTGTGAGGATTGTCGGCAGCCAAGTGAGCATTCAGTGTGTTTGTCAGAGTG

GTTACGGCTGTAACAACACCAGCAGAGTCCATGCTCAGAACTTCGTTCCACACAGATGTT

TCTGTGGCTGGGTCTTTACCAGTGGAAACTGCACCACTAACATTCTTGTAGAGTTTGTCT

CCTTTTCTGGTGATACCATTTTTGGCATAGCCAATATCAGATTCCCACTCCAAGATTTGT

TCCTGAGCAACCAACAACAATTTTTCATCAGAGATTTGGTACAAGAAGTTTTGCCATTGG

TGAGGCTCCTTCTCTACCTTCCAGCCTTTAATATATTTATTGGGCTGGAATATCGGATGC

GTGGTATCTAAATCGGGGTCTGTTGCTTCCCCGTTAGATGCCCAAATCTGACTTAAATCA

CCAACTCGATTTGTCATATCACTCTCGCTGGATAATCTTTTGAATAGTAGACTAACGCAA

TGTTGCCTGAATCATCTTCCAGCTTGGCTGTAATGCCAGCAGGTTTTATATCAGGAATCA

TGAAAGACAAAAGAACACGGTCGGCAGTTGGTAAAGTTTCATGAACTTTGAATTCCAGCT

TCAACGGGCCTTCCACAATCTCCATATCCAATTCCCTACCCAAGACCAAAATCACAAAATA

CAATTAAGTCTTCAATGCAGCAATTGGATACAATCTTAACAATGCGTGCTCTAATGGCAT

TCTTCAGCTGAGCATCAGTGCGAACAAAATCGCCAGAACTCCGGTCAGAATCGGAACGCA

GAATACCACCAACAAGAGGGTTGTTATCATCACCAGTTGATTCCGCAGCAGGGTTCTCAT

AGTAACCAAAGAACCCAAGTGCAGCCGCACCATACAAAGTACGGCTTGCACCAACTATGT

AAGCAATATCATCAACCATAACCCCAAAGGAGTTCTGCAAGATATCTATACTTAACAGAAT

CAGACATAGCCGATGAAACAACATCAAGTTCATCTAAGAAGCACTGAATATACTTCTTGA

GGTTTGGGCTGTTCTTGTACTGAGAAAGTAACAAGTCCATGCCATGTTTAACTTCGGCCA

TCTTAATCCTTATGGAACTGTGGTGTCCGTAACTTTGATGTTCGCGGTTGCAGTGCTGGC

ACGCTGGTTGATATCCATTTCGAGCGTTGTATGTGCAACATTCGCAGCAGTTAATCCAAC

CTGCAAGTCATCTACCTGAATGCCTTGAACAGACGCAAGAATTGGCGGAATCAGATAAGA

CCAAACAACTGGAGCACCGGGCTGCAAACTGTCAATGTAATTCTGAACAGCATCCTGAAC

AGCAACAACAACATCGTTAGAACTCAAGTTAGCAAGTAGGCAATCTTTTGACTTTAATATC

AAAGAAAGTCGCAACTTTGGAAGTGCGGCTAAACTTGATTGTCTTTGGATAACCCTTACT

GTCCTTGAATGTTGAAACAACATCACCATAGGTAGGAACACCACCAGCTTTGTTGTCGTA

GATGCGGCGAGCAATATCGTTCACCGTGCCACCATCAACTACCACCCAAATAGTATTTGC

TGGCTGAGAACCAATAGCAACATCGGTATCATTATCACGAATACGAATGTAATCAAGATT

GAGGTCAGCGAGTGCTGAATATATTGCTTCCGCTGTCGCAGTTCCTGAACCAACCGTTGT

TTTGTCACGGCGAGAACGGAGTGCTGGGTCTTTCTCATAGTCAATACCAGTCTGACCTTC

TTCCGGGTTTGTCACAGAAGTCAGTGAAAACATTTCCGCCAGAGACAATTTTAGTAACAGT

GTTTGCTGCAACATAGATTTCACCCTGTTGCTCGCAATATGCTGTTACGTCTCCCGGAAG

GGTTACATCAATTTTGGTTTTTAAATGTGTGACCTGAGTCGTCCCCAACAACTAGTCCAGC

AGGCACCACAGAACCATTTGCACCAGTGCACATTACAGTGACCTGAGTTGGTTTGTTTAC

ATACCTTATTGTGTTGGTTAATTCACACACATTATCTAAACCAGTTCCTTCAACAGCACC

GGGACGATAGGCATTGAAACTATATTGTGCCTGTTCCCAACATTGAGAAATTCTATCAGC

AATAATGCCAATAGCTTGACCGTCTGGTGATTCTGGTGAAGTATCGAAAGTTGAGCCGTA

AGCGCCTTTGAACGCAGAGTTCAAATCATCCAAGATTGCTTCAAGCGGCTTGCGGACAAA

TCCCTTATCCGTTACACCATAGGTTACTGTCGCCATTCCACAACCTTCTGAGATATTGCCA

TAATCAGAAAATGGCTACAAAGTTAATTGTTAAGATTCGGGCTTTCCTATCTGCATCAATA

TCTATCGAAAGAAGTTGACGCACATGGTTGGTGGAGCGAATTATATTTGCTACAGCAACT

TGAATATCAGAATCCCGAACATCTTTAGTAAATATTACTTCATACCAAGGAAGTCCGAGA

GTGCTGTCTTGTTGCCATTCGCCAAGCAATGTCAGCAACCGGCATTTAACCAATTGTGCG

ACATATTCAACCCCACCAACGCGAGTTGTTCCACGACCAATTATGATATCGTGGTTTGCA

TCTAGCTTTAGGTTTCCGGACATTTATCCATTCTTCATTGGGCCGACGTTTCCGCCTTCC

GGGTTAGTGTGGTTATGACCATCTACTTTGATTCCATTCAGAGAATAAGAACCAAGATGC

TCAACATTACCGGTTACTTTGAAGGTTTGACCTGTACCACCGGAGATTGATGCTCCACCT

TGAACGGTGAGGGATTTGGTGACTGTTGTGTCGCCATCCAATGTAATCTTCGGTGCCTTA

ACAATCGCTTCTGTCGGAGAGTCGAGCTCAATCGTACCATCCTTATTAACAATGATTTTT

GCTGAATTGGCGACAATGTTAATGCCACCACTTTCATTCAAAGTTACACGCTGACTTCTA

TCAGCATTTCGAAACTCAGCAACACCATTATTAAAAAGCTTTTATTGCTTTCGGAATAGGT

TGGTTCCCGACAGTACATGACGCCGCGTTGATTGAATACAACTGGCTAAATGCAGGAGCA

GGTTTTCCATTTATTAATCCTGCACTGTCTTTGCCTTCGACTTGCCAGTGTGCAATTCCT

CGCTGAGCGAAATGAACCCACACTGGCGTACCGTCAGGAACCGGATGCGTTAAACTCCAA

CCACCACCTTGCGTGAATTGAACCGTTACATTCTTTAATCTGCTTTTTGGAACAAGAGCA

TATGGCTGTTGCATTCCGATAAACAAATTTTCAATTGCAAGTTGCACTTCACAGGTTTGT

GTGTCGGCATCAAAGTTGTAAGCAAATCCCGGATAACCTGTATTTATATCTGGAGCACGA

GTGTTATTTTGACTACCCACGTTCAAGCACCGATTCGTCAATAATGATTAAGTCGAGATT

TCTCCAACTCGTCACTTCGCTTCCGGGATACGCATGATTGTTCGCATAAAGATTTGGAAG

TGGTGTCGAGTATTGTTTTAAAATATCAACACCACATCTGAGAGGTATGCCATAGATTTT

ATCCGTGGAAGACCATGCCAAATCAAAACAATAACAATTAAGAAAAGTATTGAGATACAG

TGCAGACATTGAAATACTCAAACCATTAAACTGGAATGAAATATCCTTTGTCACAGATGT

AGGAATTTTCTTAACATACCCAGGAGGCAACAAACTCATGATTTCGTTGAAGGCTTGTTG

TTCTTCCTTTACTGTCATAATATCCTTCCCTTGATTGCTGAGATAGGTTGAGACAATTTA

GCAAGCTTGTCCTTCAAAACACCGAGCTGTGCAGTTGGGTTCGACAAGGAACCAAGTCCC

GGTAAAGGAACTCCACCAAGTACACCAACACCAAGCCCTGTTGCAACTTTGGCAAACTCA

GAATAATCGTAGGAGTTGTCGATTGCAGTAGCCGCAGCTTTAGCAATGTCATCCTTACCA

ATTGTTTGCAACTCTTCCAGCGTAATCTCAACCGCCAGCATTGAGGCAGTGAGGCTTGTCA

TGCTTGGTTCGTATTTTCTGTTATTACACAGTTCAAATACTTTCCAAGAATGGTTGATATA

TACAACTTGGTGCCGGAAGTCCTTAACCCATTAAATAAATTGTAGGTGGATTCAATTCTG

TTTTCTGTTTCAAAAGCAGAAGATACCACACCATACAATCCACCAATTTGAGGGAGGATT

GGATTGTTGAAGATTGCACCACTAACCACAGATAAACCTTGAGCAGACACAGCCCACATT

GCAGAGTTCTGCATGTTTGTTGCAACTGCTTCAAGTTTGAGAATGCGGTTATGATTGATG

ATGTGTTCAGACACAACAAACCCGGTAGACACAGGAACCTTTGTCACCGTTGATTGAGCA

AGATGAGACTCACTAACCATCGCATCAAATTTAAATGAATTAAACTGACTTGGGTCTGGT

GTAGATGTTACGATGTTCTCCAGCTTGGTTCTGGCACCCGAAATAGCTTCGCCAATAGAA

CCAAGAATAGAATCATCTTTATCCTGAACTTGAGTTGCAGGACCAGCCGCCCACATGATG

ATTGATGGATGCGGTCCGTAACTGAACTTGCTTTTTTAATTTGGATGCAGCACCGCTCAAAA

GCTGTGCCAACATCACCAATTATATCTGACATTAGAACCTCTAGTGTGCTGACGCCATGC

CACTTCCAATGTACCAGTTCTGCCAGTTTTGCTCTGACAAACCTGATGCAGTGTTTCCAC

CGAGAACACCATGAATTATGGTTTGGAAGTCTTGGTCATGTGTGAAACCTCTGTGTATCA

CCGTTATGATTTGATACTTGTCTTCCATTGCGTACTGGAATAAAGAGTCAACACGGTTCA

GTGCCTGACCACGACCGGCCACACTTGTTACACCATTTGCCAGCAATTCCGTTCCGAGTA

ATGGAGAAACATCCATAACCATTCCGGGCTGAATATCGCTTCGAAGATACATTGCCAAAT

CGAAAATACCAATGCCCGCTATTGGGAGTTCCAATAACTGAGTTGGCATCAATTTTAACTG

GCTCTCTATCTTTAGCCATTCGCTTCATGGCATCAGAATCACCATACGTGTCTGAGAATA

TTTGAATCTCAGATGTGGCGATATAGAAATTTAAATTGTATTCTTCGAGCAAATTACCAA

ATTCTTTTATGAAGGTATCATGGAATACACGTCCCATAGGAAGTTTTGTTGTGAGTGTTG

TTCCAGACACACCAAAAGAGGAAACGGTATTATATCCATAATCAGTACACATAGACTTGA

TTGCATCGTTCAATGTTGAACCCGCAGGAATATCCCGCATTTGCTTAAACGATGTAGCAA

CTGCACCTGCGTTTGATACACAGAATAAAGTTGTTATATGCTCGGGAGCTTTCTTATACC

CCACAGCATTTGACAGAATGCCAGAGAACAACGTTGGAAGATTTTTATTCTCTTCTATTG

TCACCAGAGCATTTTGCCCTTGTGCAACCCATTGTTGTTTCACACTTGAGCCTTGCAACT

CATCAAGCCATCCAGCACGTATTTCAATCAAGAGGTCGCCAAAGGTTTTATTCTGCAAGA

ATTTAATTTCTGGCAAAGACAAGTTCTTGATTGTTACTGTTGCTGTATCAGCAGCCCAAC

CTAAAGTCGAACGCACCTCAAATTCAATACTGTGTTTTTCAAATACAGTTTTTATTTTTG

GATTCTGTGCGTTTGTAACGATAACCTGTACTTGTCTAATCCACACAATGACACCTTCAT

AAATCAATTTCAAATAAAGTTTAAGAAAGCGCGGAGCCGAAGCCCCGCACAAATCTCATC

GACGCTGTGCAACAGAACCGTGATTTGAGTTGATTGTAACAACTTCACCACTGTACGGGT

TAGTAACAGAAGCACGAGTGCTTTTGTCATTAACAGTTGCATTGATGTTCACAGTTACAT

TCAGATTCTGTTGACCATTTGAGCCAGCATCCCTGAATGAATTTGCGTTACCACCATTGA

ATCCAGCACGTTCCATGTCTCTTACACGTTTCTGTGCTTGAGACGGCATATGACTCAACC

AATCGTTTCCATATTGCTTGATTGCACTATCAACAGTTCCTTCGCCATCGGTGTATGCAG

CATTAGCTTTACGCCAATCGCCACCGTAACGTTTCATCATTGCATCTAACTTCTCACGAC

CAACACGGTTATATTCTTCTGGAGAATCATTTGCCGCTGGACGAATGCCGTAACCTGGGT

CACGGGCTGTAGAAGGAAGAACCTGCATTGAGTACTTCGCACCAGACGATGAAGTAACTG

GATTACCATGAGCATCGAAGTCTTGACCACCTGATTCCAGATTGCGAACCAAGTCAATCA

TGTCTCCGCCAGCACGGAAGGCACGTTCAGCACCCGCCGCAGCCTGCACCATACTTCCAG

ATAAACTCTCACCAGCACCACCCAAACTTTCGAATGAATAACGAGGAACAGAATAATCTG

TGGAAGCAAGAGCACGATTGCGTTGTGCATTTTCCCAATTGGAATTTGCACCAGAAACAT

CTTCAGCAGAACGAGCATCAACCAGGCGACGAGATTGGTCCATCGTTGCTTCACTTCTGG

TAGCAACACGAGCCATACCATCAAGGCCAGCCATTTCCATAGCGCCAGCAATTCGAGACT

GACTCCAGCCACGTTCTTCGCCACGTTGACGAATAAGTGCAGCTATCTTGGTTGCATCAC

CACCAGAACTTTGAAGGTCGGCAGGAGTAATCAATCCACGGGTTTGCACTGCAATTTGAA

CAGCAGCATCAGGGTCTCCATTTTGCATACGGTTGTATACAGAATGAATTGTTTGATTTA

TTGCACCGGCTTGATTTGCATTCAAGCCCATCATTTCCAAACCCTGAGTCTGTGCTGCAA

ATGCAACACCAGAAGTCATGCCTGAATCTTCTGCAATACCACGGTAGTTTGACATTGTTG

CAATGCCTTCACCACCAGCTTGCATAGCTCCAATAGTTCCTCGAAGAATTCCGCCAACCA

TCGGCAGACTTTCGATTGCATCTAATGTGCCATTTTGCAACCCACGCAAATTGCCGCTAC

GGAGGCCACCATAGAGCGAGCCGCCAAAGTCATCCATCCACCCACTTGCACCGCCAGAGC

CGCCACCGTTGCCGCCTGCGCCGCCTGAGCCACCACCAAACATTCGTTGGAAGAATCCAC

GAATACCACCGGAGCCAGTTGAGTCGGCAAATCGGCGTCTGGCATATCAGAGCCAAGCA

AGCCAGCAACACCGCCAGAGGCTGCACCAGCCTGAGCGTGGCGCTGTTTTAGCTCCTCCT

GAGCACCTAGTATGCCTTCTTTGACGGCAAGCGCCATGCCCGTCTCAGCCCCATTTCCGC

CCACTGGCACAAGGCCATTACCGGCACTGGTCACAAAGTTCGGAATATAGTTTGAAGACG

GTTGAGCAGCGTCATTGTAGGTTGAACCGCCGCGCCCAATGAATGGGTCAGCGTCAGTAC

CAGCAGTTGCAGAGAAACCACGATTGGAACGTTGAGAAACATCACGCCAGATGCCTTCGC

CCTGCATTACAGAACGAGCACCTTTGATTAACGCTTCTTGGTCAAGGTCTGGATTGCTTG

CAACAAATTCACGACCGGCAGCAAGACGACCACGAATAGAATCGAGTCGAGTTTTGTTTC

TGCCATACCAACTTGGGTCTTGGTCAATCCATTGGTCGCGAGTTTCACTTCCAATTGTCT

GCGAGAATAAAGTTCGGTTCCTTCCAGAAACCAACATGCCCATTTGAATCTGGTCCATGT

AGTCTGGATGGTCACCTGCAAGCAAATCTGCAAACTTGCCAGCACGTTCAGGAGCTTAT

GCTCCCACAATGCGTCATCGCCAATTAAACCATCTGGAGAATACATCATTCCCGGATACT

GGTCATTGGTGATTGAGCCAACTTGTTGGATTTCTTGACCTAATCTTTCTGAGACACGAA

TACGAGCTTCATCTTCTCGCTTGTGGCCCATCGCAAACATTCTTTCTGTGAATGCGTTCT

TACTTCCATCACCGCGCTGCAATCCAATGCGGTCAATCATTTCTGCCCAAGGTCGAGTAT

ATGAATTACTACCCAACATCGAGCCAACAGTGGAACCAGTTATGTCATATTGACTACGGA

AGTCGTGCCATTCCTGAGAACCTTGTTGGAAATTATGGAATCGAACAGTTGAACCACTTG

GACCACCATCAGCAACATCACGGAAGTTTGCATGAGCAGCATCCAAGTTTGCAATGGCAG

CACTTTCACGACGAACACGAGCAGCATTCCAGTCGGTTGGTTCATTTAAATCAGCAGACC

ATGCAGTGCCAATCTCAGAACGACGTGGCATTCTTAAATCAGGATTGGCAACACCAGATG

GAGCTTGTGCAGCTTGAGCATCCATGCGGTCATAGTTGTTTTCAATTATTGCTGCAATGT

TATTTCTGTTGTATGAAGTGTCGTCAAGGTTTGCATAACCAGAACGTGCATCTTCAATAC

CAACATAACGAGTTGCAGAATCGAGAAGTAAGTCATGCTCAGATGTTTCATGACGATGTG

GATTCATTCCAAACTCGCCACCACCTTTATATGAGCCTGCCAAACTTTTCAGCGTACTAC

TTACTTCACTTCGGATTGCTTTTGACGCTTCATCCTGTCCTCCGCACGTCCCAGCAGAG

AAGCGACAAGAGAATAATCGCCACGACCACTACCAACAGCAGAGCCCATTGAGCCAAGAG

GCAAACCAGCAGCATTGAGAGCTTCGCGAGGATGTTGAACACCACGCACAATATCAGAAC

CAAGCTGAGGCAAGATGGAATGATACCCAACCTTTGCACCAGTGGCAGCAGCATAACCAA

CTTCAAGGAATTGACCTCCCATCGCCGAAGCCAAACGCGTTGCAACAAGGGCAGCATCTT

TCTCATATGCGTGTGGATTGTTGTAAGCAGCTTTGTTTAAATACATGGATGAAAGGTGGT

TGTACGAATCGCCAACATGGCCTTTATTCGTAGCATACTCACCCATAATATCACCAGCAT

AATGCCCAGCAAGTGGTGGCATAATGTCTTGGTAATCATCCCAATGGTTATTTAAATAAC

CTTTTAATAAATTGAAATCTTGCGCAGGTAATTCAGGATTTGGTTTTGGCATATGCAGCC

AAACATCATCGGCTGAAACACCAAGTTTTGCCATGTAGCCATGAACAGCTTCTACATGAT

GAGACTTACCACCAGACATAATATGATGGAAAGTTTCGGTATATCCTGCACCAGCTAATG

ACTGTGCAACACGCGCTGCATCGGAAATGGATTCTTCTTCATAACCGGTGAAGCCTTGCA

TGTGGTCAAAGCCACCCTTCTGCACTTGCGTGTAGTAATTGGCTATTGACGTCCATCCAAC

CGGAAGCCTGATGCTGTTCCAGCTGTAAAACCATTTTGGACTTCGAGAGTCCGGGCTCAA

AATTCAATCCGGCCTCAAGACCAAGAGCGCGGAGAACATCCACGCCCATGCCCATCATTT

CTTTTCTGCTAGCCATTACTGTTCCCGCTCAACTGGAGGATTTAATTTAACTCTTAAATC

TAAAATGTCGTGGAATAGGAGCAGGTCGATTAATGAATAGGTTCCATCTTGCAACTCGTG

GAGTCTGCATAAAGAACTGTCTTCAATAATTGGGCGATGCAGAAAATACCGAATGTCCGG

GAATTGACTGATTAAGTCGTCGGAGTCGTATTCTTCTGGCTCGCTTCCATAGCCAACCGT

TCCGCTTCCAAACGCTCCGCCTCCGCCAATTTGAACTGTTCGTTCATTTCGAGCCCTAGC

TTGAAAAAAGCTTTATAGTTTGCTTCAAGCACGAAGGCAAATACTTGACAAGCTAACATG

AGCTCGCCATTGAAATGGATATCGAAAGTCGCCATAGTGATTGGCTGGCCTTCAATGTTT

GCATTACAGATTACACGCTTCATCAAGTCAGTAACAACTTTGTTATCTGCCTGACGCATT

ACTGCAATGATGTCACCGAAATTGTAGTTGTTGTCAATGAGTGGGAACACAGTGGTTCCC

ACTTTACCAATGAGTTCAACATGCAAGTCCAGTGCACTACTCGCCGCCAGTTGGCGGGTC

GTGAAGAGTTTGGTAACTCCTTCCGAAGTCTTAAACTCTCGCGTGAGTAATTCACAAGCC

ATTCTGTTTCTCTCTTATAGCTTAAACGCCAATCTCTGGATAAGAACCAGTCTTAATCTG

GAGACGTTCGACGTAGATTGCCCACAGGTTTGAGGTAAAGGAAATACCACGAACGATTGC

AGGCTGTTGGAGGATTACACCATTGACGCCGTTAACAAGCATGTCGCCCATCTTATCGAC

AACCTGAATCTGAATTGGTTGCCACAGAGATTTATTGCCAGACAAACCAGTTGCCTGAGT

CAATTGAGCACGCTGGTACAGAATCTCATTCCAATCCGCGGTTTGCAGGAGTGGGAATGT

AATCACACCAGACAAGTCAGCAGTTGTCGCAACAGCAAGTTTGCCATATGCGTCCATTAC

TGGAAGATGCTGAGGTACATTTCGACGTGCAGTGATAACTGCCTGACCAGCGGTGAACCC

GTCAACACGGGCACCATCAATCAGCAGGTCAGTATTATAGAATGAATATTGCTTCACTTA

GCACATCCTTACTGTACGAAGTTGCCAGAGATAACCGCACCTTGCAGCGCACCAGAACCA

ATCGCAATAAAGGAAGAACCTTCCCAAATACGTTTGCCTTTCTGAGATTGCAACAGAGCC

AAATCGGTTGAGAAAACTTTGTAGCCGTCTGGATAGAACACGCCTTCGTTGTCGTAACCT

GGGGCGATTAAACCATTGGTCACAGCCAGCTCAAGAGCCAGAGTGATTTGCTGGTTAACC

ATTGCTACGCCGGTTTCAGTCCAAGGAACCTGGTTGTTGACTGATAGAACAGGTTGAAC

ACATTCACCTGAACTTTCTGAGTCATCCAATCGACGCCCTGAATTGTATCAAACCAAGTG

CCATCTGCCATCGCACCATTGTAGAACATGGTGTTTCCGCCAACATCAATGAATGCGTTG

CCGTTGTAAGACTGCAATGCAGCAAGTTCAGTTGGTTCAAGGTTAGCAGTGGTGATTGAT

GGACCTTGTTTGAATGCCAGAATCAGTGCAGAGTTGGCAACGTTGAAGTTGACAGTTGCA

GCACGACCGAGGATTGAAATTTCTGGATATTCCACGCCGCCATTTGAGGCATCATACACG

CAGATTGTCCGGCTAAGGTTTTGAGACTTAGCTTGAAGAAATAGTTGGTAGTGTCACCA

GCGGTCAGAACTTTTGGAGAGGCAGAAGCCAGACCAAATACTTTACCAGCAGATTCACAC

CATGCAGCAGCATCCAGCAGAGGTTGAGTTTCACGAAGAGACTTATCTACAGCAACATAG

TAGAAGAACTGTTTTGTCTTGGCAGCAGCTTGTGAAGCTTTCGCAAGGTCATTCGTGATA

CCGGTTGAAGCAACACCCGGAGTTGAAGTTGCACCAGTGGCAGTCGTCAGTTTCAGAGCT

GTTGACAACGGAGTTGCAGCAGGAGTTGAAACAGCCGAAGCAATGCCAGAGGTGATTGAT

GTAATTGTGAATACACCAGCCGCCTGAGTTACAGCAACACCTGTTAACGCACCTTCAACT

AAAGTTGCAACAGCAGCCAAATCGGCAGCACCAGACAAATCAAGAGCGGAGATTGTCTGA

GGGGCGCCATCAATGTTCAGGGGTCATTCCACCCGCAGTGATGCTTTCAAATCTGCCAGA

ACAGCAGCACCGCCACCAACAAGTTTGCCGGAGGTTGCTGGAGAACCGGAAGTTGTACTG

ATAGAGCCAACACAGAAAGTTTTAGGTTGTAGGCTTCTGGCTGTACCAAGCAGTTGCAGCT

TTCTGGATTTCACCTGATGGGAAATCTTCATCCAAAGCTTTTTTGCTGGTATAGATGCGC

AGAGCATTCTCACCCATTACAGGTTGAAACTCTTTCGTCATAAAGACGATTGGACCGTAA

CCATCAGTCGCCACTGCGTTTGGTGCAACCGCAATGCTAACATTGATGATATCGGAAATT

GGAATGGACATTTGTTATCCTTTAATCGTGATGACTTGGTCACCATCAACAAACTTACCA

CCAACAACTGCATCGGACATGGTTCCAATCTCGGAAAGTTGTTCACGAAGAACATTGAAT

TGCATTTTAATGCCTTTCCTTACTTCCCAGTTTGTTTCCAGTTGAACAGTTGCGAGGTTC

AGCGCCTCTTTGCCTAATGCTGCGAAACCATGTTCGCGCAATTTAGCATTAACATCAGGT

CGATAAAATGAATTATCGAAATCAATATACTCCTGACCGTCCCGACTAAAGAGGATATAA

AATGTCAGGATTCTAATACCACGAGTTCGAAACTTCTCAACACCATTGTCATCAATGAGG

CGAGTTTCATCGTAGCCCGGATTAGAACTTGAAATACATTTGACCGCAGCATATTGACCT

TCCGGCCTTGGTGCATTCAATTGCATTTCGTATGTGAACAATGGAATACCCACACACACA

TCGACGATTTCTTTCCATCGCCTGAATTTCGCGCTCTTGAATATCCATTATTTCTCCAGAA

CTTTTGCAGCGATTACGACATTGAATCCTGCATCATCGTACTTATCAATTTCAACCACTT

TGTATCGGAGTCCGTACACAGTGATTATTGATTTCATCGGCATTTGACTTCTGGAGTGAA

TTCGCATAAATGCTGGTTGACGTTCACCAACATCAGTAGCCACCAATTTCTGGCCACCAA

TGCCTGAATCCCTGTCACCATATGGAATGGGTGTACAACGAAACTTCTTAGGTGGGGCAT

ATCCTTCCCCAACCCAATTATTCTTTCCGTCGTAATGCCCAACCATGTAAAGTCGATGG

TGTGTTTTGCAGTTGTAAAACGATTAAACGCCAGCTTTTGATTAATCATGCGAACCTCGG

ACCAGCAAATGCCATCCGACGCCAACGCACATAATCTTGACCAAACGCCGTTGAGAATAG

GTCTGCTTCCTGATAAGGAACTTTGTCCCAAATACGGTCAGCAAACTCCACCTGAACATC

ATCTACATCTGTTCGAACGACTGGCCCAGCAGACATCCCGCCTGAATCTTCATCCAAAGT

ATCACCCAACCCAAACTGGGTTGCACGATGCGCAATTAATGCAGATTGTGCTGCGTCATA

GAAGTTGAGCCAGCGTCCATCATCCGTACCCATGATTAGCACGGAATCTTCTTGCAGGAT

GGCGAATCTGTCATCCGTCAAAGACGCCATCGCAGGATAGCGCCGAACTAATCAGTGAG

TGTTAACATTGATTACGCCAGTTTGTCGTACAGAGCATCAATCTGCTCGTCGCTCAAATC

TTTAACGGAAACACCGTTCTGATTCAAGACAGCGAGTTTCTGCTCTTTGGAAATTGACGG

ACGCTCTACAACAGTGAAGTCGCCTTTCTTAATCTGCTCTTTGAACAGGTTAACAATTTT

AACTTCGCCGGTTTCGTAGAACTCTTTGATGAGGACATTCTTTTTGTCCATCTTAACTTC

CGCTTCGCCTTCAATTTCTACTTCAACCATGCGTTGTTCTTTGATGGTTGTCAGAGGTTT

GCAGAGAGCTTCGAAGACTTTGTCATCCAACTCAACAGTTGCACCACCGGGAATGTGAAT

GAAGTCAACATCCGGTTGACCATTTTTCTTAACCGGATAGAACAACTGCACATTACCGCT

GAGGTTGTTTTTAAGTTGCATCTTTTTCTCTTATTCGAATTAAAACAAAGGCGGGATATTT

CACCCGCCATTCACCAATCAGATACCTGAGTTGATGGAGATTGCAGCAGGGTACATTACC

TGGAAACCAGCGAAACGACCGCGACCTGGGATTTCATATACCAGACCGTGCAACTGCACC

GGCTGCCAAGTAAGAGCCAGTGGCTCACGCAGACGGAAGGTTTTCAGGCCATTAACCTGA

CGGCATACAACAACGAAGCAATCCGCACCAGAAGCACCGTGACCTTTTGATTGCGTTCAGC

GCTTTCACTTTCTCTTTAGAGTTAATGAATTCGTTGTTCGCAATGAAGAACTGCAAGATG

GTCATGTCTGATTGCTCAGAACGTGCAGTGTTCATGATGTACTGATGATGCAGAACCGGC

ATCCAAACTTCGTCAGGACGCATGATTTTCAGAGTTGAGCTGTACATTGCGCTCACCGCT

GTGGTCAGGTCTTTCAGAATTTCGGTTGGAGTTTTGCCATCGAACCAGCCAGTTTTGCTG

TTTGCGCCAGCAGCAACAGTTGCTTTGGTAACATCTGGATTATTGAAGAAACCAATGAAA

CCATTGGCATCATCGCCATACCATGCAGCAGAGTTAATGTACTGCTCGTAACCACGAGTA

GATGCCATTGCTTTACGCGCTTCCAGCGGCAGGCCAGTTACGGCAGCAGATGCGATTTCG

TCAATGTCGTAATCGTATGCAGTACCAACTGATTTCACCGGAATGGAATACTCTTTACCA

GAGATATTTTGATTTCGGCAGGTCAGTTGCACGAGCATTGATTACTTGCGCTTTACCAACA

TGGTTGTAGCTGCGATAGGTCAGAGTCTGAACACCAGCGCCGCCAGTTGAATCAACACCC

AGCGCATCACGAGCTTCAAGGTCAGGGTACAGAGTGTCGTAAGTTTGAGCTTCAATGAAC

TCAAGTTGACGCTGGAAGAACACAGCATCGTCATCGCTCAGTGTTACTGAGGCGTTAGAC

TGGATAGCAACCAGTGCATCATCCAGTTCGAATTCTGAACCATCGTTCAGTTTAATCAGC

TTTGGCATCTATTCAATCCAATTATGCAGCCAAGTTATTTGGCCGCAATTAATTCAGGTT

ACGGCTTGACCGCAACACCTTTCAAAGTAACAGTACCTGCTTTGGTCACACCACTCGCAA

GAGTGAAGGCCAGAGCAATGTCGCCAGCAGTAACATCATCAGCGATAGTCAGCGTTTTGG

CTGTGTTATCCCAAGTTACTTTGGTGCCGGTTACAGCAGTTACATCAGCTTTAGTTGCAC

CATTCGAATAATCGAACAGAGCCGTGTCAGCGATTACATCCCCGCCTTTCTTACCTGTAA

CATCACCTTTACCGGTTACCGTTTTAGGTAAGACCGGGGCTGCTACTTTGGGAGCGCGAC

AACGATGTGAACTGGAATCACATCACCAGCGTTGGCTGGATAGCTCAGGGCTTTGATGTT

AGTTGCTTTGGTGTAACCAGCAGCAACGCCGCCAAATTCACCAGTTGCGCTAACGCCAAT

ATCCGCAGAAGTGATTGCAGTTTTCAGCACGATGTTGATTGAACCTTCCATCATTACAGC

TAGTGGTTGACCAACTGGAATGTTGATGGTGCCGTCGCCCGGACGTGTCTTAGATTCCAG

CACGTTCTGACGCATGGTAATACCCAACACTTCGCCGGTAACATGGCCGACTTTGATGGT

GTTGGATTTCGCACCCTGTTGAACTGCCAGACCATATGCAGCTTGTGCATCTTCGGTCGA

GAAGGTCAGACGGGTTGAGTTGGTGAAAGCTAAACCATACACGTCACCAGCAGCAGCAGG

CAGTGTATTAATGTTAAAGTCTTGCTTTGGCATTGACTGAATCCTATTTATTAAAAACGAG

CTTGGCGACGAGCAAGAGCTTCTTCGCGTGGGCTTAGTTTCTTCTTCAGCTTGTTCCTGAG

AACCGTGCTTGATAGAAACGGAGAGTGCATCAGAGAGAGTGACTTCATCACTATCTTCGA

GCGCCATTTCGAAACGACCATTAACGTATTCGTCAGATTTCTCTGACATATCGGCATCGC

GAAGTTTCGCAACAACCATACGTTTCATATCCATTTCGGTCTTGCCAGTGAAGTCCATAG

ACGCATATTCATCACCGAGTTTGGCAATATTAACCAGCAAAATCAAAACGAGTTTTGACAG

CGGCATCAACACGAGTCTGAATTTCTTCATCGGTCAGACGGGCAGATTCAGCATCAGCCA

GTTTGGTAGTCAAGGCATCAACTTTGGTTTGCAGTTCGTCGCGAGAAGCTTCGAGTGCAT

CGAAAGAAGTCTTGTCTACAATTTCAATCTCTTCACCGGAATCACCGATGCGAGTTGTTT

GAGCACGACCACGCACAACAATTGCAGCATGATTCGGAACGATTTTGACTTTATCGAAGT

CAACACCTTCGTCCGTGGAGGCTTCCAGCAAAGCGTTGTGGCCCAATGATAATTGGTCAA

CACCCGAATCAACTAAGCGAATCGCAGATTCGTCATTGAGGACAATTGATGCAGCCAAGA

AAGAACCATCAGCAACCGGACGACCTTCAATGAAGCCCTTCTGCAATTCCTTATTGTTCT

TAATGCTTACATCTTGTTTCGGATGACCAATAGTGATTGGAATCGAACGGCAACCTTCAA

TGGTCGCTTCATCGAACAACACTTCTGGCTTCGTGCGAACACGACAGATTTGGTCTGGTG

GCAGATGTGCAGCAACTTCACCCAGCTCTTTGGCTTTATAGAGCATAATGCCAGTGCGAG

CAATAGTGCATTCCGCAATCATTTCACCAGAATCGCGCAGACGACGAGTTGTTAAAGTTG

CTTTGTCGGTCAGAAGAACGACACCGCCTTCAACAGTTTCTTCGTCGTTCAGTTGCACGA

ATTGGTTTTGCTTAATTTGCATTCCCACTACCTTTTGGCGGAGTTGGAGGTTGAGCCGGT

GGAGGGTCAATTGGGCACGCACCAAATACATGGCTGTGTTCCAGAATCTTGTTGGCATTC

TCACGAGTGATGGTGCCAGATTCAATCAATTTTCTCCAACGCAATGGCCAAATCAGATGCA

CGTTTTGTTTTCTCCGCGCTTGACTCAGGGAAGATATCACAGAATTCATAAGTGAATTCT

TCAATCCCAAAGTGGGCACATGCAATCGTGTCGAGAATCTCAAGACGAGGTTCGAAGATT

GCAGTCTGTAAACCAGTAAGCAAGTCAATATAGTTTACAAGGTCGGATTCACCAGTAGCA

TTCATGCCGTCTGGCGATGCTGAAAGAAAACGTGTTGCTGGGATACCTACCGCTGCCGCA

ACGATTTTCAAGTATTCCCAAATTAAATCTTTTACGCCTGACAAAGCGATAGACTTGGTG

GAGTATTCTTCCGACTCGTCAAGAATAAGTACGTTGTACAAACTCTTGGCTTGCTTCATG

AGTCGGAATCGTTTCATTACTGAACGCTCACCTTCTGGATTTGTCAGAAGAGATTGCAGT

CCTGCGACCGTCACCACATCAATGGTTGCTTCCTGTGCCAATTGTGCGGCAGCAGAAGCA

GTGGTGTAAAACTGGTCGATGGTGCTCATCAGTGGTATCAACACTGAATCCGAGTACCAC

ATGTTTCTTTGGAATTCGAACAACGGCAGTTCGGTTCCTTCGAAACGTAAGAAACGACTA

TGATGAATCATCCCAGGGTATCCGCTCAGAGTGTAATACTCTGGCAAACCGTAATGAGGT

GAAAGTGCATCAAGTACAATACCACCAGCCGCATGTAGTCGCGAACGGTCAATAATTTTC

AGGGATTTGACACATCCCTTCTTCAAATTATCAAGACGTAATGGAGTATCAAGTCTGCCC

GTACCTTTGAGGTCGAGAAGCATAAATGATGTTCCGTACAATCTTGCCCATTGGTAGCCT

TCACGGCACAGCTTACGCAGTTTCAATTTCTTGTCTGCCAACTGTCCTTCTGGCGTACTA

ATTTTGCGCCAAGTTCGCGTCATGTCTTGAGGAATAATGGTGCAGACTTTTTGACTAACC

CAATCTTCGCGGAAGCGATTCATGAGCTGACGGTAGTCTGCATTTTTGTTGGAGAAAGTC

CACCGATTATAAACCGATTTATCTTTGTCTCCACCCATACCACTTATGACATTCTCAAGA

CCGTCACCGACTTTAATCGAGCCCTTGGGCCGATTCTTTTTGGAAGACATTATTTCTTCC

TTTTGAGTTTAACCCCGTTGCTTTTCACGATTTCTACTGCCTCGCTTGAGCTAATACGGA

GTTCAGTTTCAGTGCCCAAAGTTTCGACAATCACCAGCGTTTTGCCAGTTACTGCGTCTC

GAATTTCGAAACCAGTATTGTTTTTTGCTTCGGGCATCAATGCGAGTAATCACACTCATTT

CAAACCCACGCAGAGTAGTTGGCGTTGTTGCCGGAGAATTCAATTGCGACCATGTCTGAG

ACGTTATCGCAACAATCGTCGTGTCCAGTACCTGAACCCAAAGAAGTCATCCCAAGTATT

TCACGTTTAACATGTTCAAGATGTTCATGTCCAGCAGGATAATAAATCTTGCCAGTTGAG

AAATATGGAATGGTGTTAAGGAATCGTGCTACCTTATCACCGCCAGACTTGTCACGAGGA

ACAGGCATAAGCCTGATTTGACCATCACGAACAAATTGCTGGTTTAGGAATTGACCAGAT

GATTGTCTTCCATGTACAATGCACGGGGAATACAACAAGGCCATCTTGAATCAAAGATA

TTACACTTCTTCCAGAACTCGTTTATTGCCAGCTTCAACTCAGGAACTTCATATTTCCCA

AGCATAATATCCAAGCAATATACATCACGTTCTTTCGTGACGCCCCAATGTTTCAACACT

GAGTAGTCGAATATTCTTTCGCAGTAGATGCGGTATCAGCAGTGATGAATGAACGGACA

ATACGGCTCCGGTCGAATGTTTCAGGATTGTATTCCTTCCACCATTCTTCTTTGACCAGA

CCTGTACCATTTGCAGTTGGGTCGCCAGCATACTGGCTGTTGTATGTGTATGGATTCGCT

TTCTGCATTGCTTCCAGAGATTCCAAACTTTTACGAGAAGGCCACAAAGCAGACTTTTCC

TCAGTACGTTTCAAATCATAGAGGATTGGAATCGCATGAGTGTATGCCTGTTTCTTAATA

ATCTTATCGTAATATGCAGCAGAGCCAGTTTCTTTCTCAACGATTGCTGGAATGTTGAGG

TAATGATATTTATCAGCAGAGCCACCACGAAGCAAGTAACCAACAAGGTCACTGTCGTGA

ACACGCTGCATGATGATGACCATTGGAGTACGAGCACATTTTTGTAACGTGCCATCAGGC

TTCGTTATCACACCATCGTTGGCTAAACGAGACATGAATGTGTTGTCGTAACGGTCATTA

ATTTCCATTCGAACTGTATCAGAGTACGCATCTTTTGGTTTGATTACGTCATCTACTACA

AAGCAACCGGCATAGTTCTCAGCCAACAGGCCAGCACCTTTACCAGTCATTTTACCGCCC

GTTGGAACAGCGTGCATCACACCCTGCTTCGTAGTACCCCAACGCTCAAGAGAACGTTTG

TTTGGGTCGATGCGAACATTTGGGAAGATGCGCTGGAACAATGGTCAAGCATTACAGTT

CGGATATAGCCACTGGATTCCAATACAACGTCTTCCGCATAGGAAGTAATGATGTTGTGG

CTCGTCACGTTATGACAGAATGAATACAACGGCAATGCAATGGATAGAAGTTGAGTCTTC

GAATGTCGAGGCGGGATTGTGATAATCACACGGTCGATTTTACCATCAACTATTTGTTGA

CCTACATCGAAGATAACATCATGGAAGTCTTGAGACTGAAATGGGAATCCCATTTGGATA

TGAAATGACCATTTGCCAAACTTCTCAAAACTTGAAGTTAATATTTTTCTGACTTCATCT

GCTACCTTTTTCGGTACAACAGTTGGGTCTACGTTGAGTGGGTCAGTCAGAATTTGCTGG

ATGAGTTCGTCGGTTGCAGAGTGTTCGTCAGTGTCGGCTTCAATTGTTGAGCCAGCAATG

CGGTCTGTCTTACCGTCTTTAATCATCTTTTCGATTTCACTGGCATACGCAGTGGAATCT

TTAGAGTCGGCTGCTTCCAACCTTTCTCTTTGTGCTGTAACCAAAGAGCGCAGGACAGCG

TTATCTTTTAAGCCCTGTCCTTCAATCTCTGCATCCAGTTGCTCAGAGTAATCTTCTGATA

ATGTCTGGATTCGCCGCGATATACTTACGAAGGATTGGCAGTGACTGCATACAAAAGCGT

GCACGGCTAACTTTTAATAAGTCGCCGTAGAACTTCAACAGATGATAGACAACCGTGCTG

TTGTATTCATCACCTAAATCGTTATCCATTTGTTTGCCTTTCTGGTAATCGCTTAGTGGC

GTCTATTCTAATAACCTTCTCTAAATCAGGTTCAATTCTTTTTTGCAGGCGATTCAATTC

CATGAATGAATCACACACTGGACATTGCATTAAGGAGAAGTCATCTTCACCAATAGCATA

GCCACATTCCTTACATGTGATAGTGCGCATAAGCGTACTCCAAGAAGATAGGAGGCCAAA

GAGACCTCCCAACCATCGGCGTGCGCTTAGTATTTAATATCGTTCCACAGCATATCTTCG

AAATGTTCTTCGAGTGCTTTCTGATTCTCGCTAATTACAGCTACAATTTTGAAAGGCAAG

ATAAGCGGCTTCACCGCTGACATTAAATCTTCACGGCCACGCTTGATACGCTCAATGTTC

CGCAGTGCATTCTCAGAGTTCGGAATCATAAACACAATTACATCAGCGCCAGTTTTATTA

CGGGCATCTAACGCACGAGTGATATAAGTTCCGCCACCGGCTGAGGTGACTGTTGAACGG

AGGTAAACAAAGTTTACGCCGACATTATTTTTTACTTGCGGGTTGCGCAGGTACACTTTC

TTTTGTGGCTGGTTTAGCATCTTGAACGGGTTTCCATGAGGTTACAATTTTGGTTTCAGT

AACCTGCTCAACTCGATACGCGCAGTCAACCATACTCCATTCAATGCCCCAGCTGTAGTC

TGACGAGGCATCCATAGCCCAAGTACCAACTAAATCAGCGGCAGCAATCGGAAGTTGGAA

TTCATCCACCATATCAATTGCAACTTCTAAATTAACTTCAACACCTAAAGTAACATTGCT

TGAGCTATGTTTTCCAGGAGTCGCGTCAATTTCAAAAGTATCGTCAAACAGGTATTTCAG

GAACTTGATGAAGTCACCACCAACAAGTCCTGTTGAGGTATGGTTCAGATTATTCATCGT

CATCATCCTCGAAGCAGTCGCCGTATCCGCCCCAGTTATCAACACCACCTGCTTCCAGTG

CATCTAACTTTTCGAGACGAACATCAGCTTCTTTGATTTGGTCCAGAAGTTCCAGAGCCA

TATCATTCTCTTCTGCATTTAAATGACACAGAAGACTTTCAAATTTGAGTTGTAAACACA

TGCTCGGATTCTCCATTGAGCAACTACGCGCGACCGGTTGAGCCGAAGCCACCTTCACCG

CGTACAGTTGAGGTCAGATTTTCCTGCACTTCGAATGTTGCCTGAATAGCAGGAACAATC

ATCATTTGTGCAACACGGTCAAGTGGTTCAACATGGAAGACAGCGCTGCCATCGTTGGTC

AACTTGACTTTGATTTCGCCACGATAATCTGAGTCGATAACACCGACACAGTTTGCCAGA

CGAACATCGTTCTTGAAGCCGTGGCCACTACGTGAGTAAATCAGTGCAACGTAACCTTCG

GGAATTTCTGGCTTAAGGCCAGTGCCAACAATGGCAGTATCACCGGGATAAATAATTGCA

CCTTCCACAGAACGAATATCAGCAGCAGCAGCGCCGCGACTTTCATACGATGGAAGTTGA

GCCAGCGGATGAGCAAGCAGGAAATTGACGTGAGGGACTTTATTGGGACACATTACGCAG

AGACTCTCTGAATGATTTAATGGCTGATTCCAATGTGAACGCCAATATTAATTTGGTC

GTCTGCTAAATCCACATCAATGCTTTTACGCTCTGGTGTATCTGGCAGTTCGACGTATGA

ACGAGAATCGCCTTTGAAATCAAAGCCGTCTCGATGAAGACGAATAACAAGCACGCCGTC

AAAGCAGGTGTCAATGCAGCGAAGTTCGTCTGCAAAACCACCATCGGGATAGATGACATT

AACATCGCCATCCATATGCCCGCAGAGTGTTTCCACACGATTAACTTCAACTTTGCCAAA

GTAATCGCTGCCATAACGGGGCTTATAGATTTCTTCGCTGACATAGATGAGCGCTTCACG

AGGAGTTCGTCCGCCCAATCCCGCCAAGGATTTCGAATCTTTAAGTACACGGTCAGAGGC

GAAGTGGATGAACTTATCCAAATCAACCTCAAAGTGCTTAGCAGTGTGCGCATATAGTGC

ATCCTTAAACTGATGTTTACGGAACACGCCCTGCTCGACAATCTTATTGGCTATGGTATC

TTTGCCACAGCCCGGTGGGCCATTTAAGACCACACCGAGTTTTGATTCTCTGCATCAGTCG

AGACTCTCTTCTGACTTATCAGTGCGTAACCGTACCAAAGATGGGAAGCGGAATTTACCA

GCTTCCGTTACTTCGCGGTAACGAACCTGAATGATTTTACCAATCAGTTCGTGACGATGT

TCCCACAACCATTTGCGAATGTTGTAATCGCCAACAATGTTGTGGTCTTGAAGACAGTCA

AAAACCATTGAGCCAGCAGAACCGTAGTTCGTTGCGATTGAGCCACATACACCTTTGTTC

TTATTAGTGCCTTCTTTGAAGCCAGTAATCTTAACATCGGCATATTTATATGGTACAATC

TTCCACCAAAGGGTTTTTCCTTTCTTATCCTTGCAACGAACGATGAGGCCTTCATGACCA

AGTTTCAGCATTTTCAGCATCAACTCATGAAGCTTTTCATTGCTTGGGTCTTGAGCCCAT

CCGAGGTAAAGACGTTTGTCGATGTTGCCATCAGACAGTTCGTACACGTTTTCCTGCGTT

AACGGCGACGCTGGTGGAGCATCGCTGCCCAAGATGCTTGAGGTCGTATTCCATGAATCG

CGGAAGATTTCAGCATCTTTGAAAGATAAATGGTCAAGATGGGGTAAAGGCTTGGAATTG

CGACTCCAAACTCGTCCGTCTCTATTCCTAATCGCCCGAATCCCATCAATCTTGATGTAA

ATTTCGCAACGACCTTTGAGTTGGCAATTTGGGTCTTCCCAATCATGGGCATGTGCCTTC

GTATAAGATGTTCCGTCGTCCAATAACTTAATAGTCATTGCGGGAGTACCTTCTTAAGAT

ATTCTCGAACCGCATTCTTGCGCCCGATTTTGGTTTTTGGGACTGGATTAAAACCAGCGA

GTTCACATTTCTTAATTTGTTCTTTATCAGACAATTTATAGAATGCGATTACTTTCTTCT

CAATGATTGCTTCTCGTTTGGTAACGTCGTAACGCTCCATGTAAGTTACAATCCCATGAC

AATCATGGCAAAGAAGTTCCAAGTCTGATTCCTGAACCAATAATAAATTGTCCATGAATT

CATGTCGCTTGGATAAATCCGAAAGGCCACCACATTTGTTCTTGTGGTTGACCTCGATTT

CTTTAAGCTTGAACAGCTTTTTGCAATGCTCACACTGCACATGATAGATTGGCTTCTTAC

TGTTGCCAACCCTCAGTGCCACTCGCTTCTTCTCAAGAAGTGCAAGTTTTGATGGATGCT

TTGCCCAAACACTTCTCAGTGCTGATTGAAGCCAAGCTGTAAACCTGGCTTCGGTTTTGC

AATAACCATACATGGCAAATTCACATCCCACTAATGCTCAAGCAGAATGCCACACGAATA

AAATCACGCCGAAATGGTTTTAGAATTAAGGTCGAAATATTCCACAACCTGTGGATTATA

ATGCCAAAGTCGAACCTTGTTCCTTCTGCCACCGTATTTGACATATATAGCAATCGGCC

GTTCACGAGAAGTTGTTCCTGCCAACCATCACCAAAGCGCATAACATACTGTGTAGCTAC

GGCTTTCAAACCGTCTGCATAGTTGTCAATTTTGTCCAGCAATTCGAATGCTTTCTACAGC

ACCGACACCGACACGCTTTTGATATTCTTCACCAGCCTTTGCGCCAGTTTTGTACATCTT

TGTTTCACTAACACCACAACCCATTACACCATCGGTTGAGTCACCAGTGAGGCACTGCCA

AAGGAAGAACTTCGGACCAAAGCCTTTGATTTCTTTCTTCTTGGTTTTACCATTATCTTT

GACGATACATTTAAGTTCGCCGAAACCAGAGACAACAGTGATTTTCTCACCTTGTGTGCC

AGTTTCCCAACGATAATGTTTGACAACTGGACCACCCGGACGACCACCGATTTGTTTCAA

ATCTTTGTCGAGGGATGCAACCCAAATTTCGTCATAGTCATCAAGAGTCAATGGGCGAAT

GGTTTTAATCGTTTTGATTCACCACGTTTACCCATTGTCATTTCGACTTTATTACGACA

CAGAATACCAATCAGGTCATCAGCTTCCAGACCATCTTTCTTGATACCGTTATAACAGGT

AATCAAAGTTGCTTTGGCATTCTTCAAATTCTCTGGTTTCCATACGCCATCACGGTTTGC

TTTGTATGTATCAGCGATAACCATGCGCATGTTTGAATCACCAGAAATCAGACAAATGTG

TTCAGTTGCCTGACAACGCTTTTCCATGAATTCCATTTTGGATTCCATAGACATCTGAAT

TTTTGCAAAAGAAATATCTTCGGACTCTTTCCCGTACTCTTCTGCCGCGCAATGGCTGAA

TGCCACCATGTCTCCATCGGTCAGGAGGAGAACTTTCTTCTTACTCATTTTGCAAAACTC

CAGTAAAAGAGGGCGCAACAATCTTGGTTACGCCCTTTTACCAATTGGTAAATACATCTA

CCCAACGTAGATGCAGTGTGTTACTTAGAAAGGTTGACCAGCAGCGGCATCAGCGCCACC

ATTCTGTTCAGCCAGTTTAGCTTTCGCAGCAGCCTGCGCAGCAAGTTCAGCAGCCAGAGC

TTCATCAACCTGTTCTGGAGTTGCTTTGTTCTCAACAGGTGCATCTGGAGTTTTGGCAAT

GCCCAGCAGTTCCTGCAACTTAGAACCGTTGAAGTCCAAGTTTGCCAGAATGCGGTTCTT

AACAGCCCACTCTTGGTCGCCACCCGGCAGCTTGCCGAATACTTCGAGGTCAGGAGCACC

CAAGTCGAAGAAGATGGTTGGATTGACCAGAGCTTTCGCATCACGTTTGTCTTTCGACTT

CATTGGCGCGAAGGTTGAGATTTTGTTTGCCATCTGACCAGCATTCTTACCACCTTTCTT

GGTGTACTGAGTCAGGTTAACTACCAGTGGTTCGCCCAGCAGTTCATTCAACTGTTTGGC

TGGATGACCGGCAATACCAGCTTCTGGATTCGGTTTAACTTCAAATGCGTCAACCGCTGC

CATGAATTTGTAGATGTGAGAACGGTCACCCATAAATCCATCTGGATTGTAGGTAAACTC

GAAATCAAACCAACGTGGTTTGTCCAGCAGGTCTTTACCAATCATTTCGCCTGGCTCGCC

ATCAGGGTCTTGGACCATAATGATTTCGCCTGCTTCGTTGGTTTCTTTCATGAACTCATC

GAGTAGCTCGAAAGTTCCCAGCAGTTTGTATGCTGGTTCTGGATACTGACCTGAACCCGG

TTGTTTACCGAGGTCAATCAGACGTACCAGACGGGCTGGATAACCACCTGATTCTAACAG

TGGTGCTTTACTTGGTGCTTTACCGGAATCTTTGGTTGGTGCTTGGAATAGGGCCATTTT

GTTTCTCCATTGAAACAGTTTTGTGCAAATTAGTGGATTGGCATGATTGCCAGTTTGAAA

TCATAGGATTCCAAAACAACTAAAAGTTCTGGAACATCCTTTGAGTTGGAAATTGCTGTG

AGGAAATCTTTAGCCCAATCTTCTTCGACTGTCTTGCTAATAACTTTCTCTGTCAGATAT

GCTGCCACAGCATCCTTCGCAAGGATGGTGGCATCTGCACTAAACTGAACCAGCGTCATT

TCCTGTGCGTCATACAACGCGTAAATGAGTTTAGCCATTAGTGAATCTCTGAATAGTCGT

CGCCGAATTGAACTTCACACGCAACATCGCGGTTCATCTTCAATTGTTTATTGGTGCGTT

CCATTGCTTCTACACCCACGATTTGTTGCCACAGTTCACGATTACCTTTGCGAACTTGCA

GAATCAATTCATCATGGAATTGTCCAGACAGCATTGGGTCACGTCCATAACGTTCGTTGC

AAATTTCAATTACAGCATTGCACCAAACATCAAAGACATACGCGCCAGTACCTTGACAAA

GAGTTGAGAAACGGTCTTTGTCATTACGCAGTGAATACCAGAAACCATTTACCGGATTCA

ACTGCCACATCTGACCATCAATCGTTTTCACAGTTGTGTTCGCTGCTACTTCTTTGATTG

ACCAATTCAGACGCCAGTAAGCTTGATGCAGCTTCTCAGCTGTAGCCCAATCACACTTCG

CACCACGTTGCACTGTCGCAATACCCGCACCATACTGACAAGCATAGTTTGTGGTTTTGA

ACATTGGACGTTGAGGAACCTTCTTGATTCCATCTTTGTGTGCCTGTGATTGTTCTTCGG

TAATGAATCCACCGATTACACCAATTGCCAAGTGAGCATCATAGTCTGGTGCCAACTGTT

TCTTGACATATTCAGGGTCATACTGCCACTGGAAGTGGTGCTTGCAACGGTCTTCAAGTG

AAGATAGGTCTGAACCAAGTTGTTCATAATCTTCATTTGGTGCTTCCAAACATGCCGCGAA

GTTCTTTACCACCGAACACACGAATGGATGGAATATTTACCAGACCACGATGTTTCAGTC

GCAGCGTGTTAGTAAGACCAGCGCAACTAGCAACAAGGAAGCCATCGTCGGATACATCAC

GGAGCCAGCCGTTGACAATAGACAAGCGGTTACGATATACACCGAGGCCAATAAGATGTT

CAACACCTTTGCCGGGATTATTCTTCCGCCAGTTCATGCAGGCTCGGACAAATATCTGGAA

TACCATCATCATCCTTAACTGTGATTTGTGGAATCTGACGAGTTTCATTTGTTTCCTTGT

CACGAACAAACTTGAATGTTTGCGGCTTCCAGCCAAGACTGAATAACCAATCTTTTACTT

GAGCATGAGACGCAGCATTTGGTTCGTCGTAACCTTTTAAAACTTTTATGACGAGGTTTT

TGTCCTGCCAATCAAGACCATTTGCATCACAGACTGCTTTCCATTTGGCGCCTGTTGCAG

ACAATGTTCCATTTTGCTTATGACACTTGGCTGGACGATTCTTAAGTGCATACTTAGGAA

CCTTTGGCATCGACGCTTCGAGTGCAATTTCTTTCTCCGAAATCATTGGCGCAAGCATGT

CGCGAAAAGCTTCTGCTTTCGGTACATTCAGCTTCCACCGGGTACGTTGTTGAATGACTT

GCTGACGGCCTTTGCTCATCAGGTATTCGACAAAGCGTTTCACTTCTTCCGGGGTGCGAT

AAATCGCTAAGAGTTGGGAATACTGCTGTTCCCACAATGCCATTTGAATACGGCAGTCCT

GCATTACACGGAAGTTATATTCTTCCTGAGTTTGGTTTTCCCAATTCTTCAATTACAGGTT

TTGGTACACCAAATTCTTCACCGTATTCTGCCAAACCATGCCTCATACGACGAGGTTGCA

GGTACCAGCTAATGAACATAGTGTCAATAATCGTTACTTTGGATACATCATATCCAAGGA

AGGCTAATGCCTCCTTATCAAAACACAAACCGTTGTGCATGATGAGAGTTGGAGCCGTGT

CGAGAAATGCCTGGATTCTACTACGTTCAGTCCACTCCATACAATTTTCAACTTTCGTTA

ATGCATCAATCGCACCCATGTTGTGCAAACGTGGATGTTCCTGTTTCGCCATTTGCTCTA

ACAAACCAGTCGTTTCAATGTCACTGGCAGACATTCTTTCCCAAGGATAAATCAATTTTG

TCTCCAAGGATGGTTGAGGAGGTTTGTCCAAGCTTCTGTTTCACAACAGTAGGAGGACAA

GACCTTAGTTATTGGTGCGGGGTTGTTTTCTTAGTGAGCTGAACGATTTCTTTCGTTTCA

AACAAACTATCTTCCCAATTGCGTTGAATAATACGACCAGAATCTGATTCGTAATATGTT

TTGAAACCATCTGTCTTACCACTCTTACGGGCTTTAATACCGCGAATGATTGAACAGTTT

GGGTCAATCGCCTGACTGTTTCTTTCGAAACCAATCATGAAATGGGAATATTTTGATGCT

GCACGAGAACCAGTGAAGTCACCCTTCTTAATACGTCCACCATCTTCATGAGGACGCTGA

CCTTTCGGAACCGGGTTAAGGTGAGATAGGTCGAGGATACCAAACTGATATTGTTCGCTT

AACTTAACTTGGTCGGCATAAAGCTTACCGAGGAAATCATTCTTCTCAGACGCTGAAATA

CCTTCTGACAAAATTGTTAAGTTATCCAAGACAAACATGTCAATGTCTGGACCAATTTGA

CGCAGGATAGTTTTGATGCCTTCCCATGTTTCATACGGGTCTTCACCTTGTTGCTTTCTA

TCCCAAATCTCCATGTTCGCAAGCATTTTACGACAAGTTGCGCGGAACTTCTCTTCATCG

AATCGTGGGTCTTGTCTGAATGAAGGTTCCCAATATGGGAGTCCATCATGCAAACCAGCC

ATTCGAAGCAATGTCTCTTGTGGAGTTTCCTCCATGAAACAAGTGAACACACCCCAATCA

TGTTTAACGATGTTGTGTTCTGAGAGTTGACGAGCTAGTGTTGTTTTACCACCACCTTCT

GAACCACCAAGTGTGATAATTTCGCCGCGACGTTGACCGAACATCATGTCTGTCAATTTA

GACCACGGAGTATCAACACCCGGAGGAACTTCATCGTCAAGTTCCAACATTAATAATTCT

TTATTCACCAACGCTGTTGGCAACGGACGTGCAGCACGGAAGATTACCGCATCACGAGTT

TGTCTTAACATTCCTCGTTTAAGGCAATCATTGGCATCGTTTGCCGGGAGTTGTGCAACC

ATTGCACCCGGTAATAAACGGCATGCTTCCTTCGCTGCTTTCTTGCCCGGTTCATCATTG

TCAAAGACGATTACCACGCTTTCCCAGCGTTGCTTAATCTCTTCGGCTACGCGAGCCAGA

CATTTATGAACTGAATCTGAACCATCTGATAAGGAAATGATTGCGAAATCAAGTTCCTCA

TATGCTGTACCACGGTTCAAGATGCGGAGAATCTGACGCAATGCAATCGCATCTTCTTCA

CCTTCTTGATATACAGAGTTTTACCACCAATGAATTTGGCAGCTTCCCAATTGTATGGG

TCATTCTCCTGAGTATCACCAACACTCCACATCACTTTCTTGTTCAGAAGTTTAATCTTC

CAACGAACAAGTTTGCCGCCACGAGTATATCCGTGGGCTAAAGCATTTGGAGTTGTACCA

TCGAATTCCGAATACAGAAGTCGAACACGAGCATGGCTCCAGTATTCTGGTTCAATGCCG

CGATGGTCGAGATTAAACACCGGACAAGAAGTTATCTCATCAAGTTCTTCCTGAATTTCT

TCTGGAGTTTTGACGTGAATTTCTTTTTGGGTCCGGTGGATTGTCACCATAAGGGTTGGGA

ACGAGAACAGAACAGGCAAAGCAATAACCAGAATAATTGCGAGTTCCATCCTCATTTTCG

TTCAACCAAACTTGCAGGGATTTCCCGCTTGTTGATTTACACGTGGGAACATCGTGCTTA

ATTTTGGCTACACACTGTTTGACTTGCATCTCGTTGGAGTCCTTAATAGTCTCTGGCGAA

CTCTCCGTGGAGTTTCTTTCGCAGAGTTGTTGCAGCAATCACCGCATCTTCAAGATGTTC

GTGAACACCGCCGCTATGTAGTTTACCATTCAAACAGACATTAACTGCCCATTTGCCTAG

CCTCTTGTTGAAACTTACTCCTTTATGTCCGGAAGTGTTTCGTGCATTTAGTTTTGCATT

GTGGTTATTCTGCGAAGTTGTGCACTCACGCAAATTATCCAAAGCATTATTGAGTTTTGTT

GTTATCCACATGGTCAACTTTCTCAACAACCTTCCTAGACTTGATAAAGTAGTTCAACCT

GTGTTCTGCCACACAGTATGTAGTGCCGCAGATGTTTAGATACCAGTAGCGATATCCAGA

GGCCAAATATGGACTATACAGCCTTGCAGATACTGCCGCAGGATACAATCTTGGCTGGCAA

TGTCTTATGCATTAACCTTGTCCTGTTAAAGCTTTCCAAGTTCTAGGAAAATGTTCTTTC

ATGATTACATTAACTTCTTCTGCAAACAGACGGGCCTCGTACTGTGCATGTTCTGATGAA

CGCTGCTTGTACAAGTTAGCCCAACCATAGAGGCTTCCTGTCCACACCCAAGTTGTAATC

ATACCTTGTGGAAGAACCATTCGAGCTTGCTCTGGAGCAACACCCAAATCAATTAAACCT

TGGTATTCACGAACAGCCGCACGTTGCACCGCAGCAATGAGAAGTTTCATCTCACTGGCG

TAGTCTTCACGCATGGGTTGTGCAGATTGAGTCATCAGCTTATGCGGGCGACTAAGAACA

AGTTCTGGTTCGTAAGTTTCAATCTCAATCGCTACACCATCTTTATAGCGGCGCGAGATT

TCGTTCCATGAGAACCCAACCTGATGTTTACCCAATTGACGGGCAAGGAAGATTGGAGCT

CGGCAGCGCAAAGTTACATGAGCATGACGGAATGGAGTTACATGATTATGCTCGCCAAGA

AAGTTGACAAGATGTTCATCACGCTCACTAAATTCGTGAGACTCCGCACCATATGAGACA

CGTGCAGCATTGACTGTTGTTAAATCTGTGCCCATCTTATCGACGAGTACAGCTGAAATT

TGTTTGGACATTAATGAACTTCCGGTTCAACTTTGATTGGCTCAATGTAACGAGCCGCCA

GCATTGCATCTGCAATTGCGTAAGCTGTTACCGCGAGTTTTGTTGCATGAACTGGTTGAT

GATAAATCTCAGCACCAGTTTCTGGATTTAACATTCTTTCCATCGCGCGAAGTGCAATAT

CATCACGAAGATATTCTAGATTTGCTTCATCTGCCTCAGCGATAAGCTGCGACAGAACTG

CATCATTGTTGCTCATGAATTCTCTCTCAAATGGGTATTAAAACGATTACTCAGCTTATG

GCTCTTTGTTCTACACTCTACGTTCACGGGAATGAAAATAAGCTATCGTACACTTTAACT

GTGCAGAGTGTAGAACAAAAAGACTTTGATTCCTAACCGGCCAAGCGGAATCACTTCTTG

ATATGGGAGAACATCACAGGCTCGTAACACCTGTCATATTTTTCATGGGAGAATGAAGAA

ACCCATGACATATGAAACCCTGCCTTGCATATTTCAATGTTGCGTCTCGGAGGTTAACCC

AATCGCCAGTGAGGAGCCTGACTCGTTAGACTGCTAGACAGTCTGGTACGATGCAACCGC

AACAAATCTCACCACCGCGTTTCTGAATTGTGTTACAGAAACTGTCCGTCTGGATTTGAC

TCCAGTATGGCCAGAGCGCATGTTACGTTTTGAACAGACGTAAAACTGTATCGAAGGATAT

GGTGGAGACGGTTGGACTTGAACCAACAGCGGTAGCATTTCAGCTTGCGTTTACCAATTT

CGCCACGTCTTCCGTAATTTGGCAGGCTTTTGTTTATTTACCGGAGCCCAATAACCGGGA

TTCAACTTTGGTGAGTGTGTTTCTCAGACACTGTTTTAGGAAGTGACCACCTGAAGTCTGG

CAGCTTCATTTTGTTTTGAGTCTCGTTGTTTAAAGTCGCGCACTCCCTCGACTATGTTGA

GCAGTTATTTTCCATACTCAGGGCTGGTATCGAACAACTTGTCGGGTAAGTTCGAATTCA

TTCACTCCATCTACTTTGGTGAGACAGACCATCGTGACTGACAGTGACAATGCACGGAG

CAAATAAGGGGGCGGCATTGGGAATCGAACCCGAGACACCAGTCTCTGCCACCATTATTT

GTTGCCGGTTTTGATAACGCAGGTTTCCAGCGAGAACTGCGAGGCTTGCTTTCGTTCAAT

TGTGTCCAGAGAGAACAACAGTGAAGACTCCGGTTCGTTTCCTCGGCTTGATAGGCCACA

CAATTTCACTCAGGCTGTGTACCACTCTTTGGCGGCACACATTTCTTTTCATAAATTAAA

TTATGAGAAAGAATTTGTCGTTTGGTTTCTGGAGTCAATAAAGGAATTTCCTTCTTCGAC

AACATAATCAAACTAAAACTGGAACAGCTTGTATCAACAACTCTAGTCTCGTGTGAAACG

CTGCAACTCGTCGTCAACACTGGTATCGCTAGAGCGATTAACAGACTCAACAACTTTCTT

TGCAGCCGTGGCTTCACGGACTTGTGTGTCGGCAACAGCTTTTGTGGATTCAATTTCTGC

ATCCTTCTTTTCGACTTCGGCTGTTTGCTCTGCCTTCTGTTTACCAGAAAGATAGGCAAC

ACCCAAACCAACTAAAAAGACGAATGCAGTCGCAATCCATCCCTTAAATTTTGCAACCAT

CGCAATCAGATTCATCGAGGATTTCCTCCTGATTCGCTAATTTCTCATCGTTGCGTCGTT

CAACTTCATCGAAGTCGATTTCGTTTAATTCAAATTCGTCCATCTTGGCCTTTAACTCCT

GGGCGTATTTTGCAACTGCCGCTTTAAACATTGGATGCGCTTCATCAAAGTGCGGGACTT

TTCCAGTTGTTGTGAAAGCCTGAGCACTTCTGCTTGAATTCTTAATTCAAACTCTTGCA

GAGAAAATCCGTGTGCTTCTGCTTGAATTCTCAGTTCTTCATTAGTCATCCCAATGCCTC

ATCGAATTCGCGACGAATTTTCGCCAGTGCGCCAACAGGATAATTTGCTTTCTTCACACC

TTTGAAGATGAAAGATACTTCTTTATGCTGCGAGCGTTTCAATAAAACTTTAATACATTC

ACGAAGAGTTTTGTTCTCAGGACGTTTGTCTTTGCGTGCCAAGGCAACAGCAATTTCGCA

AGTCATGATGAAATCATAATACTCCATCGCGAGTGACATTCTTTCGTCCATCGTGTTCTC

CATGATTTAAATTTAAAGCCGCTTGCTCCGGGTCTTAGCCGTCACTTCTATAAGAGAAGA

CACACGCGGGTGAATGTGTTGTCTCGTAGTAGTAGAGTATACGTCCCACAACTACGTCTA

TCTTCAATATTTCAACATACATGCGAGGAGTCGAACCTGCCTTACACTCTTTGTGCACAA

GAGGCTTACCGAAGATGTATGCTTCAATTCTTTATTAAGAGTAGGCGCGCATAGTCGCCT

CGTGTTACTTGTAACCTTTCGGTTCTTCTTTAGTGCTCGCGGACAGAGGATTCGAACCTC

CATCTGAGTGCGCATACCACACGTTCTTTGAAGAGCGACTTCAAATAGTTTGTGTTCCTC

GCAAACCGTCTTAGGCCCATTGAGACTAATCCGCGTCTGTTAATTCAGATACCACTAAAG

CTCTCTTAATTCACTTTGATGTTGCACATTGCTGGGCCATGACATAACGGGCATCGGCCA

TCCTCCCGCATCTAATCCAATATGCAACTCAAAGTGCTTTCGTTCACCTCCACTCTACGA

ATACTCGAACCGTAAAGCGCCAATTCTCCGAAGAATGGAGGCGACAGAAAACATACATTA

TTAATCACAGCGTACCACTGCGCCGAATTCGGTTACATGGAATCGGAAGACCACGGAGCG

AGACTAAATTTAATTAGGCTGCAACTCGCATTTCAGAATCGTTTGCATTTATTTTAAATG

AATAAAAACAATCGCTCATCAACGAAAACTTCTGGATGACCACTCTAAGAGTGCGGTAGG

AGTTGAGGTTCCTATCCGTCTTTCAGCCAACTGCAAGTGCACTTCCTCACGGATACTGAG

AGATTCACTGAGGTCTCAAGTCAGTTACTATTTCAAGTCCTGTGTGCACGGTCATCTTAC

AAATTGGATTATTCAAATCATCCAGAAGCCCACTCTCAACTCTCGGCATCGGCTCAAAGC

CTCTGTGCTGTGGTTGGCAGAATGGGATTCCGGGTATTTCAAATCCCGACAATCATATCG

GGATTTGCGCTGCGTTTACCAGACGCCAGCTAGATCGATTTTAGAACAGTGGTGCAGCTT

CTTCGCCGGCTTCAACTGGAGCCTGAGCAACTTCTGCATCAGCAGCTTTAGCCGCGTCAT

CTTCAACCACTTTCGCTTCTTTCGGAGTATCGAAAACAGTGCGCTCAACGGTCGGACGAC

CTGCTTCAACCCACGGCAGGTAGAATGCCAGGAAGTTGGTCACAGTGTTGACCTGCATGT

TGCGCGAACGGTCGATTGCACCAATGGTGTTTACAAACGCTTCAGCAGCCGCTTCAATCT

GCGCGCCGTTTTCCAGTTTGAAATCATGTGCATTCGCTTCTGCTTCGCTCAGGAACAGAG

AACCGTCGTTTGCTTCGTACTGAGTAACCGTTACTACTTTAGCCATTTTGGCAATCCTTA

TGTTGGATAGATGGACACCTGAGATTTTGTGTTGGTGTTCGCATCAGGGAATAAAAGTTT

GAAATGGAGCATTTCCACATTCAGCTAATGCCGAAGAATTGCTCCAATATTACAACAAAA

ACAAAATCAAAATTTGCGCGAAGCGCAGAACGATTTCTTTTAATAAAGAATCCATTTCGTC

TTCGGTAAATTCGGTTTTGATTGAATCGTTGATGCCCTCAATCTGCACACAGACAAANNN

NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN

NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGACCTACACCATAAACGGTTA

AGCGGTCAAGTCTGTCGCGGATTTTGACACCAGCTTCATCTGCAACTGCACGCAGCAGTC

GCCAGCGGAGTGTTCGGTTTGGAACGACAAAACCATGTTTGTAGAAGACTTCAACCATGA

GTTTGCCGAGACACACTGCTTCAACGTTCCACCCACGTCCAACTAATTCCTGCAACATGC

AGAGAGTTAGAAGTTCGAGCCGGTTGAAATGCTGAGTGCGACACAGATTGAAATACTCAC

TTGGAGTATTGGAGCGAGAAAATACTGCTTGCTTAAATGCACGTTCACTTTCTTGGTGCAG

CCAGCATCAGTAGTTCAGGCTTTATCAACTTCATTGCATCAAAGCCAGCACGTGTGGCTT

TAATGACGCGGCAACATTCATATGGTTTTAATTTAACCCACTCACCTTTTTTGGAATCAG

GTGAGAAGTGCTCCCAATATGTTACTACGTTTTCTTTTGCAAGATACACGGCGTCATGTT

CCTGCAACCACATCTTCACCGTTTCAAGGGCAATGATGCGGTCTTGCTCATTTTGCTTTG

CCACTTACAATCTCCTGCAAGACTTGAATCTTTTCCATGTTTGCAGGATTTGTGTGGTCA

ATTTCTTTGAGCGCTTTCTGAACTTCGGCGTATGTTTGGGTCTTACCATTTGCAAAGTGC

ATAATGATTCTACCAATTTCGCCGGAAGGTTCAGCACCGCTTAAAAGCAGCAACTCAAGA

GTTTGATTGACACAATCACTTACAGCTAATGCAACAGCAAAGTTAACATCGGCTTCCGTG

AAAGTTCGCTGTTTGCGTTTAGCCATCAGTGACCTGCCATGATTCTGTTATTAGCTTCTT

CTGCGCTCATGCCCATTGAAACCAACTGCACCATACATTGAGCAATACGGCCTTGTGCAA

GTTTTTCAACTTCATCAAAACCTTTGCGTCAACACCCATGCTGTGTTTCACAGACAACA

GTGTTGCCATCAAACCATGCGCCATTGCGATAGCCTGATTTTCTGGCATTCATTGTTGA

TGGTGATGCCAGCAAGTTCGTGCTCAATTTTGTTGAGATTTTTCACTACACGCAACTTCG

CGCGCAGCTTTTGTTTAAGAGTTTTGCGAGCCAAGTTTGCTCTCCAGTTCAATGATTCGG

TTTGCCAAATCACGTACAAGTTGAGGACCGAGTGCTAAATCACCACCCATCATTTCTGAT

AGGTCTTGTTCGCATTCGTCCATGTCATGTGAAAGCTGCAACACTTCTTGGATGTTTAAT

CCTTCGTGCAGCACCTTACCGGATGTTAGAACCAATTTACCGTAAATCAAAATGGTGCCT

CATTTCCATAATACGTGTTGGATTCCCACTCACGCATATAAACTTTGCGACTAACTTCTT

CGCCCATGTAAGTAATTACATCGTTCAAAGTTTTGGTTGTATACCCGCCACACGAGCGTG

TGATTATTTCAGCAATTGGGTCAAACCAGAAACCATCTTTGGCTACACCCGATGAAATAT

TGGCGTTCTTACTACCGTTGTAAAACCATTTACCGCCAACTAAAACATAGTGATATACAT

CACCATCTTTCATTGCGACAGATGTAACTTTTGCAGAATGCTTTCGAATTTTACGCGGCA

TGTTAACGACCTTTTCTTTTCTTTAAGGTTTGCGCGATGTTCTTTGGCACGGCTTGAATGG

AACATGGCAAGGCGTTGGCTTTCTGCCGCAGCATCCACAACAGCTTGAAGGTCATCACGT

TCTTTCGAAACCCTCTCAATGTCTTTGGCCATGTTCTCAATTTTCTGCAACAATTCTTTG

GTTGCATTGTTTGGAACAGGTTTACCGCCAAGATGTTCACTGAGTCGCTGACGCAAGTCC

TCAAGAATTTCATTCATTGGGTTGCTGCTAACATTCTTAATTTCCTGAATATTAGCTTCC

AAAGTAATAACAAGTCTCCGACTTGTTGCTGCCAACATCATTTCCAACGGCGCACCAGTC

ATCCATTCAGAGATATATCGCTGGGATTCCACATGCATTGTGAGGTCAGCGTTGGTGATA

AAACCTTTTACGTTGCTCATTGATTCTCCCAAGTTTTGATATTGAGTTTCAATGTTGGTT

GCCAGCAATCATCGCAGCACTTCCCAACCTGAACCCACAAACCATTCTTTACAGCTTGTT

TACGAACTGGATAATAAACCTCCAGCAAAACCAAACTGACGTGTACAGAAATAACATTTGC

TCATAGGAATTCTCTCCAATTAAACGCGCAAATGTGACTGATGGTTCCTCATTGGCTGGC

AAGGAACCGCTCCGAAACCCACTCAGCACGGGATTCTCCGTGGCGCTGGGCGTTTGAAAG

CTGGTGGCAATATGATTGCATAGGTGTAAGGCAAAGGGCCGCACAATGGCGTGTAGCCTC

AAACCGACAATGCCGTATGGCTCCCTGTGTCGCACGTGGCTGACCTCTTGCCCTACCCTA

TGTAATCACATCCAACTAATGCTAAAGCGCGGCACAAGGCCGCTGCCGTCGCAGAATCGC

TATCTATACCACTGGTTTTACCCAAAATTATCAGCCCTAACAGCTTGGTACAAATAGTCA

CATTCACCCGGTCGAGTTTACCCACATGTAGTTTCATTATGTGCCCTTAAAGATAATTAT

GATGGCTCACAATCATCAAGGCAGGTTGAAATATGTCTCGCCGGTATATTGTAGTTACAA

CTTCGCCGCACCAACCCAACATAGCGGGAATAATTTCGTATTCTCACGGTTTATTGTTAA

AAGTTAATAGTGCAAGTGAATGATAGCATAACGCTATCTCAAACAGACTTTGTGACTAA

CCTGTTTAACGATAGTTTCACGCCGTTATTATCGGTTTTGGTCATAACCACTTTTTCCG

GTAGATGTGGCTGCATCTATCGTCACAATGTGTTTATTAACCCTTGACACAATTTTGTAT

AATGTCCTTGACCTACAGAGCACACGTTCCACACTTTAGTTGTTTGTGGGAACACACCAA

CGTGCCAAGCTGGAACACCAATGTTCCAAGTGATTACAATGTGCTTCGCACCTCCAGCTA

ATGCCGAAAGCGCGAAGCGCTCACACTGCTACAAAATTACCGCACGAATTAAAATCCAGT

CAGAATCAAAATATTCGTCTGGTCGGAAGTACATCTTGAACTTGTCCGGGTCACAATCAA

CTTCAAACGCTGTTTGATTGCCAAACAAATCCTTCACGCGAACATATGTCCAGCCTTCAA

CCCATCCATTCGGGCCTTTGCCTGAACAACGGTTGATGACTTGGTAATAGTGCTGACGGT

CTTGGTGATAAATGATTTCACCACCAGTCCACTTTGTTGGCTCAGCTTGTTGCGAAGAAA

TGATTGTTGTCTTCGGAGTATTCTCCACCTGCTTTCCAATCAACTGTGATTTTGTCATAG

TCAATGTAATTCTCCAGGAATTCTGGAACTTCGTCTTTGTAGAGTTCGTCCCAACGTTCT

TTGACAAATGATTCCCAATCCTTGAACTCGCCAATGTAGCGGTCTTGAATAAGGGATAAT

GGAACATCGCAGGCTATCGCCGCTAAAACAATTTCGTCGGCATAATCGCTACGAGCGTCA

CTGATTTCTTCGTACATTTCCCAATCAAAGCCTTTCGGCTGAGTCAGGCAACGTTCGCAC

AAACCTTCTGCTGCAACAACTTCCCAATCTGCATTTGCAATTCGTTCAGCAACATCTTCT

TCAATCTCACAATGAGTTCTGAGGAAGGTTGCCATACTTACAGAACCCAATGGATTGGCT

TGATACAATGCTTGTGCATCTAACCAAATATCAGTTCCTGACTTCAAACCATAAAATCGA

ACACAAATATCACGAACATGGTTTGTCGATTCAATTCTTCGAATGTAACTATACCCGTCC

ATAACTTGCTCCGAATTTAAGACGTCCATGTAAGGACGCCGTTGCATTATTTCTTTCACT

GCTGGCCTATTACAGCAAGCCGCTGGCAACCTTTTTCAACGTATCGTGAACGTCTGTGAG

TTTGGATAACTCAACATCAACTTCTTCGGTTGTTTTGGCGATTGCTGCCTTTAAATGTTC

CGCACTAACACGAACATAGTCGCCTTCAATTTGGACAACCATCCATTCTTTACTGGTTGG

TCTATCGCACTCACGCACTGCACGATTGCGGTCTTCGTTGTGACGCTTCAACTCTTTGAT

GCGGTCTGGCAAATCTTTATAGTCCATTGGATTCTCCGCTATGGATTATGCTGGTGGGTA

GTCTACTGCATCGTTTGCATAAATGATTAACTCATCCAGCTTCACTTTGCGTGCATCAAT

TTCATCCATGCGGTGTTTTAAACCTGCAAGAAGAAACTGAGTGTAAACTTTGAACTGTTT

GACACGGCCTGGGTTCATCAGAATTATTTCTGCAACTGAGTCATCCGTAACAACACCACC

GGGAACCGGTTCTTTGAACGATTCAACAATCAAATCAAAGTTGGTTTGGTGTTCGGCACG

CTCTTCTTCCAACCTTTTATACTCTTCGGTCAGAAGGAAAACTTCTGTATCCGTTTGAGT

ACTCATGCTGGCAACTCGAAATGAGGCATGTCAACAAAGCTTGATGGTGGCTTTTGATTT

TCATCAATCCGCGACCAATTACCGCCCCAACGAATGGTGATGCCAAGCTCCTTCGCCGCT

GCAAACATGTGATGCGCGAGGATGCGGAAACGTTCGGTATCATTCCAGTCGATTGGATAT

GGAGCCAAGTCAACAGCATTGCCGGACATATGCCGAGACGTCATAACTTTGGTTGCACCC

TGTGCAAACAATTCAGCCTGACGTTTCTGACTGCGTAAACCTTCAATCACAGTCACATCA

AAAGGCAAGGTTTCAAATGCCTTCGCACAAACCTGACGCAAATCCATACGGACTTGGTTC

AGCTTTGCCTGACTTGATGGACTCAGTTTGACGTTACTCATGCCGAAACTTCTCCAACCG

GTGTTGCGTTTGCAGCTGGTGGATTTGTTAATCCATCAATTGCCTGGTCCAGCGCTTGCT

GCAAATTTGTTTGAATACCGGATAGATTGCCTTGGTATTGCATCAATTTGACCAGTTTGT

CATTTTGCAACGGCCAGAAAGATGCGAGGTCAACTTCTGTCCGTGACTGAGTTTCTTCAA

CAAAGACACTAAGAGTTTTTGTGCCTGAGTCATACATCTCAGTCAGTTTATCGACAAAAG

ATTTCTGGTCAGCAGCGGCATCAGCGGCAGCCTGAACAGCTGCGCCTAATTCGTTTAACT

GCATGGTAATCCTTTTGGATTTACGGTTAGTTTGAATTTCACTAACTCGTTATTGATTTT

CGTATAGCTTGAAACAACAGCTTCATGGCCATACATTTCCGCAGCGGCATGTTTACCGAT

GCGCTCCAAATGACGGATTACCTTATCGGTAAGTCGTGGGAACACTGCTTGAGAGCACTC

GGACTCGATAAGATAGTCGGTTCCGTCAATTTGAACTGGCTGTGAATACCTTTCAACTT

TAGCGTTTCTCCACTGATTGAAAACTTTGCCTGCACGCTGGTGGAGAATTGCCAACAGCT

CATCGGATGTTAAATCCAAATCAACTGCTAATCCTTCTCCAGCATCGGCAACAACTTTGC

CTTTCACCAAATGTCCATTCTTGACTTTATCAATGATGGCAGTTAGACGGCGATATTGGT

ATTCGAGGTCTATTTTGTCCGGCACGGCAATGTTCTCTCTGGTTTAAATGCGTTTGCATA

ATCTGTGTCTTTATAAAATTCGACATAGTATGCACCTGAATAATTCTTCCCGACTTTGGC

AACTTTACCAGAGTCGAGATTGTAAAAGATTTGGACTGGCTTGTCCGAAGGAACCTGCAT

CCAGATTCCTTTTGTCCGAACAACCTCCAACAAGATGGGATTCTTGTCTTGTTGGGTAAA

GATGAGACGCTCACCATTGCACTCAAAGGTGACTTGATGCATTGATGCATGGACTGGATG

CGAACAACCATAAACAACTAAAATCGCGAAACTAATCCAGAACATTGCTGATAATTTTAG

TACCATCTTCATCGTAATCAATCCTGTAATTAATGTTTAGCAGATAGTTACGAACTTCTG

CCCAATGGGTTGTTGTCATCTTTCCATAGAAAGGATGAGATTTGTGAGTTTTATTCACCT

TCACACGTCTGGCTAAGACACGAATGTATACTTTCTTTGTCCACTTACTGTACGAAACTT

TATGCTGGTGGAACTTAATTCGCAATGCTTCATACTGCTCAGGAGATTGATTCTCGTGAA

CAGTAATTTGATGCGTGTACATGGACCGATTGTAATGGTTAGGCCAATCACTACAATAAA

TGAAATTTTGGTAGTAGACGTACATAGGACCTCCAATTGAACCTATTCAGGCTCCTATCG

GAGATAAGAACCTGTAAGATTTTAACTGCAACTCACAGAGAATTGATGTGTTCACCAATTT

CTTTGCTGAGTGTTGCCATCTTAACCTTGATAGAGTTAAGTTTGGCTGGGTCTGCTTCAC

CGTGATATTCTTGGTCGAATTCAACAATCAATGAAGAAATCTTTGATTGCTTTGTCTGAA

TTTGAACCAGAGTATCGTATGTTGCGTCAAAGGTTTGAACCTCTTCCTTATCCATTTGTT

ACCTTATTGCTTTGTTGAATAAATTAACTTAATTGAGGTCACTCCTATCGGAACAACCTC

TCAAAATTAACTTGGTTTCTTAATCATTTCAATTTCGTTTTTCAAACGATTGAAACAAGC

ACGCATTTTCTCGTGCACCATTTGCTGAACAATCACCGAATCAACTTCGAAATTGGTGTT

TGCCAACATCACTTTTTCAGGTAATTCGTTCGCATTGTCATACTGCTCAAGCAGCACGTT

CAACTCAGACATAATCTCAATTGAATTGCTAATATGCAGATGTTTTGTGTGCACGTAAGT

TCTTCTCTGAATGTTCGTGAACAAACCTAATTTAACAGACAGTGGAATATGTTTTGGATT

CTCTGCCGGAACTAAATTAAGTTTCGCATATGGTGAATTGCGATGCAGGTAGTTTTGTTTC

TACCTGCATCAAATCACCCTCGTAATAAATAATGTCGCCAGCACGTAATTCACTGGCGTA

CACAGTTTCGAGGAATTGGCTCATTAGTTACCTATGATTTCTCGCAGCATCCTGCGCTCT

TGTTTAAGAACTCGCTGCAAATCAAAAGGGAATGTCGTCATCAAAATCCATTGGCGGTTC

GTTGTTCTGAGTCGCTGGTGGAGTTTGCTGAGCTGGAGCTTGCTGCTGTGGCTGAGATTG

AGCCGGAGCCTGTTGCTGTGGTTGCTGCTGTTGAGCACCCTGCTGAGGACGTTCTTTCTC

TGCACGTTTCGCCATCATTTTGTCAACTTCATCCTGAGTCATCAGAGTGAATGCCAAAGA

TGTGAAAGTGCGGTTGTTGGATTTCTTATCCCAACCTGAAACCCAATACCACAGGCCACC

TACATTACAACGACCACGTTGGTCAGGATGAGTATCCTTCTGTTTGTAGTCGTTTGCGCC

GATGATGCCAGTGTTTGTATCGTCGTAAGTTTTTGGTATTAGACATGATAAAGCCTCTTTA

TTTAAGTAATTTACAGTTTAAGTTACAGCGTCATTGCTGTTCTTCACATCCAACTAATCG

TGAATATTAAAAACGCACTCAATGAATGCGCTTTTGTATACAGATTAATTTTCTTTTGTACT

TGACGAATGAGTGGAGCATAGAAAGAGTAATCATTTACATTAAATCTAGATTTCTCCAGA

TATCTGTCACGACCATGCAGTCCGTGTATCAAAGATTGTTTCACACTTTGATATTCAGAC

AACATAGACATGAGATGCCTCTTTCTATCTTGCTTCCATCTGTTTATATATGTTCTCATA

TCAGAAAATTGTCGGAATATGTGAACCAAAGAAGAGCCAGAAGATTACAGCAATTGTAAT

CCACCAGCCCAAGCCACCGTTACGGGATTTGCGAGCTTTACGGAAAGTAAATGATTCGCC

ACCATAACCATTTTTGAATGAAGAACCATACCACATAAAGTTGCTCCTGGCCTATCGGCC

CACTGGAATTGGATGATACAAATGAGTCACCGGAACACGGCGCTTATTCGCAGTTGGACT

CAAATGCACAACATTGAAACGATGGTCACACTTAATTAAGCGGATATCGCCAAAGTCGTC

TTTAACACACATTTGGTAATCGTTAATGATAAACGCACCAATTCGACCATCACTGCGACT

GATATCACCGTCACCGTAACCAGCAAGAACTTCATAAACACGCCCTTTAGTGAACGCGTT

TTCTTTGATGCTGGCTTTGTTGATGAAAGCTTTGCCGATTGATTCACAGCATTCAACTAA

ATCACCTTTGTTCATTTGCGCTTCGCCTTCGACATGATTTCATACAGGCCAACAAATGAT

GCCAGAACGAAAATGATGATGAGTACAACAATCAAAATTGGCCATGCCAGCGCTCGTGCA

ACACGATTACTGAAATGACGATGTTTCTTGCTCAACCAGCATTGCAAGAATAAATCAATT

GCAGCAATAATCAGATACGTTTGTACCGTCATAATGCCGAGGATAGAATAATTCAGAAAT

TCGTTCATTTCGGTTCTCGACTTCCATAAGTTGTGCCGCAAGCATTGCAGCGATAGATTT

TTGGATTCTCTTTTTCTGGCTTGTGGAAGCAATTGCAACCACAAGAACAGTTAAAGTCTT

TTCCAGCAACTGTCAGGACGAAATTCTTTAAACTTCCGTCCTCGTTCTTAACAGCATTTG

CGTACTTCATTTGCGAGGATTCACAAAATGAACTACACCCAATTCATCAACGTAATATGA

CATATTACACCGTCCATTCGCCAAAAGTTTGCCTTGCTCGCTGCAACATCATAGTTTGGAT

GTTTTGGAGTCAAAGAACAGAAGGTTTTCTCACCACGGTCGTTGATTACGACAAAGTGAT

TTTCAGTAACACAAGCCCACTGATAATGAGCGCCAATATAAGAACGCTTGTCCTCGCTCG

ATAGTGACGTTGCATAGACTTTATATCCTTTATTCGCGGTTAATCCGAGAACAGTTGAAG

ATTTTGAAATCGTTGCAATGTCGCCAACACGAACGCGGGCTTTGGTCATTTGACCGAGTT

TGTTTCTGCGTAGAATCGCCATTTACTGCTCCTATGAAGTTAATGATAGTTTGGCTGCAT

CTTGCCGACATTTAAATAACATTTGGTATTTGTTCAGACTGCTTATAGTCACCGGCAACT

TAATCCTGCTATGAAGTAGTGAGAACGAATACAAAAGCTACTTACGTTTATGAGCTGGAG

TATCCAGCCTTTTTGCGCTCGAACCGCATTATTGAGCCATCGGGAGTTTCATTGTTGTCA

ACTCAAACTTGATATGTAAACCTTCGCGAATGGTGAAACAGGGCATAAGCCCAAAGCCGG

GATTCGCTTGGTGTAACTGGAGTCAGGACTTTAACGGTTTGATGCCTGACGAGTTGCGAT

TTGTTCTGGCTCAGAAACAATATCGCCATAACCCACATCCAGTTTATGAAAATTATCTTG

ATACAGCCACCAATGCACGAAGTTCTGTTAATTCACGCTGCATTGCTGCTTGTTGCTCTT

CCATCATGCGAATGTGTTTATCCGCAAACAGAATAATTGCTGCATTAGTTGTGATTGGAA

CATTCTCAATCAAACTTACTGCATTGTCGGCTTTAGTTTGAGCACGACGAACTACGTTAT

TAAACGCAATCAGACTTGACATTTTTATACCTCTACAGGTAAGCCAGAGTTGTGAAAGGC

TGCGCTTTCATCATCCACAGTATTCATCGAATTCTTCCCATGCTTTGTACAACATTGAAT

CAGTTGAGATATTCATGATGCCATTATTTACGAATTCGGCACCATTTATTTCAGGATATG

CGTCGGACAGAAATGCGAGGAAATCTTGGAGCGGTTGACCTTGTAATTGAATCACTGGAA

TAATCCTCCCAATTCTGCATTAAACGGACGAAAATGAGCTGTAGAACAAAAACGTGTCCT

ACCTTTATCATCAATTAGATTGAAAGTTCTCTCACTGTGGTGAATCATTGTGCCCAACTT

TAAAGCAATTGGACTCACATTAACTTCACCTTCACCTGAGACAACTTCATAACTTTTGTA

AGCAGTGAACCCGTCGAGACGAGCTTCAACATGCTTCATCACAACACGTTCACCAGTTTT

CATTATAGACCTGTGTTTGACAAGTTGATGCCAATGAGCTTGGCTTGATAGTTGCACACA

TCTTCACAGTAAAGTTGATTACCAGCACGATACCACCGGTTATCATCATAATCTCTCATA

TAGAAATCCCAACCGTGACGTTGCTCAATCCAATGCCGGAGAATCAAACGTCCTCCGGGA

GTTCGATACAATTTGTTCTTACAATCGAAACGACTCATTCAATCGTCTCCCAAAATTCAC

AGTATGTAATCAGTGATTCAATATCATCACCAGTTGTACCATCAACATCTTCCAACAGTA

CATATTCAACAACAGTTTCATCCAAACCTTTCAGATGAACTTTCAATTCAGACAACGTAC

CTTCGATTTGAACTTCGTTGGCACGGTCACGATATTTGATAAACAGAATTGCCATTATTC

GTGCTCCAATTCTACTTCACAATCGTCAAGAATCATGATTGCTTTGCTGCTATCTTTGCA

TTCGACAGCATTATAGAATTCAGATTCTTCCAAAACTTTCCAGAACACAGTTTCACCAAC

GGCAAACTGTTGACCATCTTTCAAATCACCGAAACGAGCCATAATTTTGTATCTCCAAAG

TAAATTAAGAAGGGCACCATGTGACTAGCATAGTGCCCGATGTACATCCTAATGATAGTT

TGGAGCCTATCTCGCAACTTACTCAGCCAAAATCACCGAATAAATTGAGAGATAAACTCT

TTGTCTTTCATTCACATGCAACTAATGTGGAACAATACTGTGAGGCCAAATCTCACTTAA

TTGTCACATTACACATGCGGGTCTTGTAACTATCCCAGACTCGAAACTCAAATGTATTTG

GCACATTCAAAGTTCAGAAATCTGGTACAGTTGAGTGACCAGCCAACAAAAGAAAGAAAA

AGAATAAATCTTTTAATTCCCAATATTGTCACAAATATAGTACCTGTGATTATGCTAACA

GGTTTAGCCAAACCCAGTAACCATGCGGGTTACAGTATTACATGCAGAAAACTGTGGCAA

TATTACATGCATCTATTTGTCACAATATTGCAAACGAGTCACATGCCAATTTGACCAGTC

AATCGTATCTTCGAGGTGATTAGTCCTCTAACAATCGGTCACATATTAACATGCAGAAGT

AATCGCAATATTGAAACGATATTAACATGCCATACTGCCACAAATTTTGTTACTAATTTT

ATGTCGGCTTCGGGCTTAGTTAACGCAACGAAACGCCACTGAACCCGAATGCCACTCACT

TCTCGATTTTGTCACCACTATTCCATTTGAGCCAGCAAGTTCCAATTCAATCCCGGCTTG

TGTTAGGGCCTTTATAATAGGATTACGGAACTCAGTAACTCACATGGAACATTTGGACAA

TCTCTTCTCGGAACAACAAGTTCTTACTCAGCCATGGATGCTGGAACTTTGAGTAGCCAT

CTCAGGGAGTTCTACAGTTGACTAAACCTGTAACATCACTGTGACTAAGCTGACATGCTC

AATACTGTTGTACACCAGTTTCGAACTTGACATGCAGAATTTTGTTACTCCACCTCCAAC

TAATGCCAATGTTGTCTCGAATATTAAAACAATATTGGGATTAAAAGATTTTATTTTTTA

AAAGAATAAAGAAAGGGCCTCCGAAGAAGCCCTTAGTTGTTTTGGATTATTGGCAGAAGT

AATAAGCGTGTTCGCTCATCAGGAGTTCTTCTGTTACGATATTGGTATGGAACATATCAA

TGCCACCAATTTGTTTGCTCAATGCTTCGAATTGATTATTCTTCACAAAGATTTCAACAT

ATGCCTGACGAATTACATCCATCATCTTAGGAGCATCTGCCAAGCAGCAACGGAAGCAAT

CGTGAATTGAAGTGAAACCACGAAGTCCAGCTTTCTTTGCATACTTGGCAACAGTACGTG

CAACCAATGCATCAATACCTTGAATGTAGTTAACAACAAAGGTACGAACAAATTCTTCTA

CAGTTGGCTGTGTCTCACGAATGCTCCAAGCTTTGCCTTCATTCTGTTGTCCAAAGATGA

CACGAGTTGTAGAGTCAACACGAATCGAGAATGGGTCACAGATTTCACGTGATGGGTAGC

AAGGTTTGTGAACCACTTGTCCATCAGTGTGGCGATAATCAATGTAGAGTTTAGATTCTT

TCTCACATTTGGCTTTAACAGCTTCCATCACTTTAGCGATGAACATGTTAATCTTTTCAC

CAAGTGCAGCTTTCACAGCTTCAACAGCAAGTTCAGCAAATGCTTCAACTTTATCTGCAC

CAAAGATTTCAGTAACAGTTGCTACGAAGTCTTTGTTACCTGTCAGAGCTTTCTTGCCAC

CACCATATTGAACAGCCATGTATGGAGTCTTGATGAAGTTACGTCCATCTTTGCGATTGA

AATATTTCTCTTTTACTTCATCGGTAGGTTTCATTGAAGACTTGTCTAACAGTTTCAACA

ATTCCACCAGCGACAATTGATATGGGTCAGCTGCTTTAGAATCTGAGCCAACTAATCCAG

TTGCCTGTGCCATTTGCATGTTGCCTGCAATGAATGCCAGATACTGAGTACCAGAAACATT

TTGCATCTAAGCCAAAGCCAACACGTGAATCACACTCACCAGTTGTTTCAAACTTGAACC

AATCCAATGCCAGACGAATGTAAGTGAATGGCTTCTTAGGAGCGTCACCAGAACGTAACA

TGTGAGCAAGAGCTCCAACTGGGTTCTGAGCAACACGAGTTAACATGCGAGCTTCTGTCC

ACTTGCCACCAGAAATGTCATTCAATTCAGCCATGAACATCTGGTATGCTGCACTGTCTT

TCTTCACAATGTTCTCGATGTTGTGAGAATAGAGTGAACGAGCAAAGTCAGAAGACTGTG

GATTTGGACCAGCACATGCAACGTGATACAAACGACCACGGTTATCTGCAAAGTAATCAG

AGAACAGAACATCTTCTTTCGCCATGTTCTTAGCGTGTGTTCCAAACATGCAACTCTTGTT

TGATAACATCAGGAATGTCACCATGAGACATGCCGAGTACGTCACCAACAATGTCAACCA

TGGCTGCATCAACATGGTATTCAGTTGATTGCAGGAAGTCGATACATTCTGTCATCAGTT

TGGAAGGCTTACGTTTGCCACCTTTGATGAATGGCTTGCGACGATTGAGTTCTGACACAG

CTTCTGTCAGTGGAGTGCAAGTCTCTTTAGTTGAAACGAGTTCAATCCAACGCTCAGTCA

TGGTGAATGACTTATCTTCTGCAATTGATACATAGCCAAGATGCTTAGCCAGTCCAATCA

AGTTGATAGATGGGTCAACATAGTGGAACTGTTTCAGGTCATTGTTGGTAGAGTTGGCTT

TCTTGATTGACGCTTCTGCAAGGCTAACCATCGCAACGGCTGGTACAACATCGTCACGTG

TGAATGACGTCATCATGTGGAAGATAGCGAGAGCGGCAATAGCACAAGTCTGTGGCTGCA

TGTAGCTCTGTGTTTCTTTAGCACGGAAGATAGCAGAAGTGATAGAAGGAACAACAGATT

TAACAACGTTTGAATTAGTCATGATATAGCTCCAAGGTTTTATACATAGTCAACTAAGCC

CACAAACCAGTGCCGTTGTCGGATGAAACGGATTTAGTTTGCGTAGCCATTACACTATTG

ATACCTAAGGGAGCTGATGTAGATGCGTTGTTATGCCTGCGAAGCAGGAGCTACTTTGGT

GTTGATTTGAGTCCGATAGGACGAATTGGAAAAAGAAGT

<110> REPUBLIC OF KOREA(MANAGEMENT : RURAL DEVELOPMENT ADMINISTRATION) <120> Novel bacteriophage specific for fire blight and use thereof <130> PD21-341 <160> 1 <170> KoPatentIn 3.0 <210> 1 <211> 53319 <212 > DNA <213> Artificial Sequence <220> <223> Erwinia phage Fifi451 <400> 1 agctagatga agtacagctg accgaccatc catcgcatat cccattacaa atatcacatg 60 cataatattg tagcaatatt tggaattgaa tattgttaac ttatttcttt cttaatagtc 120 tcggaaagga tgttcgcttt cgcgaccacc ttccctcaac atctaactga caagattcaa 180 tgcaccttaa tttcggcatc ttcgtcgata atcttgagca aggcttcctg catttgaatc 240 aacttttcag gagtccagat tgatttatca aggaagataa tagtgcaatg gtgttcttcg 300 gcaagatttg ccaagtattg cattagagct ttatccatta tccgttacct aaatattgat 360 aaaatttgtg atttccgacc tgttttatga acaaagtacg tccaaatttg tcagattttg 420 atgaaaa atg agtacatttt cctgtgtttt tcgtgtctaa aatcacaaat ttcaatgcaa 480 tttcaagact ttctaccaca gctttgtgct cttttgagtc aaatttccgc gctacttctc 540 taaaagaagt gttccgcttc catttgtttg aagtccatga aaactggcgt ggct gataaa 600 ttacaccaca gatggtgttt gaaaatggct taccaaactt agctctgttc agtacgacat 660 ttgcaaccat gacctttcca gcatagcttt ctcctctggc ttcgaagaag atagctttag 720 ccaaacagtc aacttcatgt ttctgacttg cagttaattt caatccacct tggagataag 780 attgagctgc aaactccgac acatgcgcat tccatttgga attcactcca gcatcagcag 840 ttgccaatgc cgaagtgttc c acgcaaata tcaggagcat gatgcaaaac aacttccgca 900 tctgtgttcc ttttatcttg ctttctgaa ccaagttaat ggattcaatt gcttaattgc 960 tgccgagaac gaatcacgta aagtagtgat aattaacttc gcacctacta caccaatcac 1020 aatgcaaaca acaa atcctg cattaggatg attaaccaaa taaccatcca gaattgggag 1080 tgagaagaca aacgttccaa taacacaaca cagcgctgca tcaagcagag cttttccaag 1140 accttcactc tcataggctc cacgaattaa agcagtaagt ccagctaaca gaccatgcag 1200 taatccatac tgttgcatga ataggaagat tacttggata gtttgtctat ccactttgta 1260 gcccttatca atcaccatat cgcatacacg ttaacgttga ccgcgacatt ctgtggattg 1320 tagatgttac aaccattttg agaaatgccg ttaaaccacg ccctgttctc accattgaaa 1380 gctgagaaga atacagcagg acttccaggg aattgcgccg ggaaattcca agttgtggtt 1440 gcatttgctg caagagacaa gttct gtcta caccacatag gaccatttgg aatcttagtc 1500 caagcaccat taccgtttgt accagactgg aattggtcta gacgtacagc atgtgcacca 1560 cttgttgcac caccaacatc aaactgtgtg tactggttgc catttacagc agccttactt 1620 tgcagcatga agttgagtcg aacattgttt acagcatagt tatcagaacc actgttcgaa 1680 cactggaatg cttgacttgc atcgccgttt atgtaggca g catacaaact accccttgca 1740 acagtcctgt cggcaacatc agtcaagttg ttgttcttct gcaaagcatt gacaattcgt 1800 gagtcattac cagcagcgac agtgtttgca gtggtgccaa catcaagaaa tgcagcacct 1860 tttaacgcag ctgtaagtgc gccga aagac caacctttgg ccatttttaa cccttatagc 1920 gttgagattt gttttgccgt caaagcacca atccagagtt taaagttttt ggctttaatc 1980 agtctctggt ttgcttgtgc ctgaacatct gcacggagga agttgtaatc acttcctgg 2040 atgtttgcat tgttggctgc aactgtacta accagaactc cgttgagata cagcgacaaa 2100 gaagttgtag ttcttacacc aacaagacga agccagacac tggttg gaag tgggattgtt 2160 ccagttgttc cagtctcaga ccagaaacga actgtgttat tcttattaac ttgaataaca 2220 ccacaaccag cagcagcacc aaatgatgca tacaattcag gagcagttgt agttccgcca 2280 gttccttcaa ggaaaacatc aacacaaatg gttgct tctt tcaaaccttc aagaggtttg 2340 tatgtataca caacacggtt gtcatattgt gtattgtttc caaagattac accattcagt 2400 agagatggat tccaatccag tgcaccacca ttttggtcgg cctgaacagt accaccgcca 2460 atgtagacat tcaaatcaga tgcaagaggc aaatagaaca caggttgctg cactgcatag 2520 gctgcttcaa cagcagcttt attatctgca aatgttgctt tagtaatgat ttt ggttgct 2580 ggagcattcc cagttttgac aaaaccttca ccagaagcat caacaccaac ttgacctgca 2640 ctgttcgcca tatagacgcc agtgtcatca gctttgattt tacgtacacc atcggcagac 2700 agaagaactt gaccagtctt catttcaaca ttgccggtgt atgca ccacc agttacagga 2760 actgcgccaa tgtcagtggc tgtaactcca tgtgggttat ctttccttgc tgcatggtcc 2820 gaaaccattt tcttgtactg agcaaccaat gcatcaattt gtgcagtggt gtatgttcca 2880 agtcttcccg gagtcagtcc atgagggtta cttgttacat cagcgatgtg acttgcgatt 2940 gccgcagaaa ttgcatcata atctgcacga gtaatctgca atgcacttgg agtccattga 3000 gcc aagtttg aacttggttt cagcgtcttg cttggaccat caagtttggc aatataaatc 3060 actccatttg attcatccca aaccgcagca ccatttcggt aggttgcatc gccaccccat 3120 tcaaacacac cacgttcagt aagagcttgc agtgttgaat ccgtcttcca ttga agatag 3180 ttcagaactt ggtatgttgg gatttcagac agccaacctt ttaaatattt ttcgtcgcct 3240 gggtcacgac gagcaaccga attcgagtca ccccaaattc ggccaagttt aggacgcagc 3300 attatctatt cctatacagc agtgatagtt aaaattgcag ccattccgtt ccatttaaca 3360 gttaaggttg cagttcctgc tttcaggatg ttgattacag cctgaccatt tgcttgtgtt 3420 gaacca acaa tctgaacaac acttgaatcg gaagttgtcc acacagcact gccatccaat 3480 cgagcaccac gaacatcgtc aggactccaa cggaatgcac caatatactg accatttttg 3540 ggagcagttg cattaggcat tacgaaaaca tctgtcgtga ctttggaaac ataccaagct 3600 gttcccggag ttgaagatgt atcatttgca atatgcaaag aatcatactt cggtttcagg 3660 ttcaacttct ggtattcagt cagtgcaaga taaatatcca taacacacaa atgcgttgca 3720 tcaggaatca atgcttgcag gaatattttc agatttggtt tgacgacaac ttgtgtggca 3780 tcaacaacag caaacttcct gatatcgcca gcatagtatt caaagtaaat tgttgcagga 3840 atactaacta atccca ccca agtcatgaat gtattatcgg caatcttcca tcccttattc 3900 ccttcgaaca ctggagcatc ggttgtttca acaatgaagt taccgacacc cattgtgtca 3960 aaagaaccag cacgaagctg catttgcaga atcggttgag gaagtgcaaa ggaattattt 4020 actgcaattt gat gtgcatc gaagttcgca acagttgtta tatcactgcg aacaccagaa 4080 gactcaacac caacagttgt atcgccatca ccttcaagga atagcgaacc accggaattt 4140 gtaatcttct ggtctggtcc atcaattcgg aaacttcccg tacctattgt taaaccacca 4200 atgtaattaa catcaccagt aaaggctccg ccactggtag gcaaggtacc aacttgcgct 4260 ggagtttcgc catgaacttt acctcctgtc tgactt tcgt ggtaaacgat tgttgcaggg 4320 ttgttcgggt cgccaaagaa tttatcaatc tcaccagact catatccacc aatatcatgg 4380 atgttatctt tgtgaggatt gtcggcagcc aagtgagcat tcagtgtgtt tgtcagagtg 4440 gttacggctg taacacacc agca gagtcc atgctcagaa cttcgttcca cacagatgtt 4500 tctgtggctg ggtctttacc agtggaaact gcaccactaa cattcttgta gagtttgtct 4560 ccttttctgg tgataccatt tttggcatag ccaatatcag attcccactc caagatttgt 4620 tcctgagcaa ccaacaacaa tttttcatca gagatttggt acaagaagtt ttgccattgg 4680 tgaggctcct tctctacctt ccagccttta atata tttat tgggctggaa tatcggatgc 4740 gtggtatcta aatcggggtc tgttgcttcc ccgttagatg cccaaatctg acttaaatca 4800 ccaactcgat ttgtcatatc actctcgctg gataatcttt tgaatagtag actaacgcaa 4860 tgttgcctga atcatcttcc agcttggctg taatgccagc aggttttata tcaggaatca 4920 tgaaagacaa aagaacacgg tcggcagttg gtaaagtttc atgaactttg aattccagct 4980 tcaacgggcc ttccacaatc tccatatcca attccctacc caagaccaaa tcacaaaata 5040 caattaagtc ttcaatgcag caattggata caatcttaac aatgcgtgct ctaatggcat 5100 tcttcagctg agcatcagtg cgaacaaaat cgccagaact ccgg tcagaa tcggaacgca 5160 gaataccacc aacaagaggg ttgttatcat caccagttga ttccgcagca gggttctcat 5220 agtaaccaaa gaacccaagt gcagccgcac catacaaagt acggcttgca ccaactatgt 5280 aagcaatatc atcaaccata accccaaagg agtctgca ag atatctatac ttaacagaat 5340 cagacatagc cgatgaaaca acatcaagtt catctaagaa gcactgaata tacttcttga 5400 ggtttgggct gttcttgtac tgagaaagta acaagtccat gccatgttta acttcggcca 5460 tcttaatcct tatggaactg tggtgtccgt aactttgatg ttcgcggttg cagtgctggc 5520 acgctggttg atatccattt cgagcgttgt atgtgcaaca ttcg cagcag ttaatccaac 5580 ctgcaagtca tctacctgaa tgccttgaac agacgcaaga attggcggaa tcagataaga 5640 ccaaacaact ggagcaccgg gctgcaaact gtcaatgtaa ttctgaacag catcctgaac 5700 agcaacaaca acatcgttag aactcaagtt agcagtag gc aatcttttga ctttaatatc 5760 aaagaaagtc gcaactttgg aagtgcggct aaacttgatt gtctttggat aacccttaact 5820 gtccttgaat gttgaaacaa catcaccata ggtaggaaca ccaccagctt tgttgtcgta 5880 gatgcggcga gcaatatcgt tcaccgtgcc accatcaact accacccaaa tagtatttgc 5940 tggctgagaa ccaatagcaa catcggtatc attatcacga atacgaatgt aatcaagatt 6000 gaggtcag cg agtgctgaat atattgcttc cgctgtcgca gttcctgaac caaccgttgt 6060 tttgtcacgg cgagaacgga gtgctgggtc tttctcatag tcaataccag tctgaccttc 6120 ttccgggttt gtcacagaag tcagtgaaac atttccgcca gagacaattt tagtaaca gt 6180 gtttgctgca acatagattt caccctgttg ctcgcaatat gctgttacgt ctcccggaag 6240 ggttacatca attttggttt taaatgtgtg acctgagtcg tccccaacaa ctagtccagc 6300 aggcaccaca gaaccatttg caccagtgca cattacagtg acctgagttg gtttgtttac 6360 ataccttatt gtgttggtta attcacacac attatctaaa ccagttcctt caacagcacc 6420 gggacgatag gcattga aac tatattgtgc ctgttcccaa cattgagaaa ttctatcagc 6480 aataatgcca atagcttgac cgtctggtga ttctggtgaa gtatcgaaag ttgagccgta 6540 agcgcctttg aacgcagagt tcaaatcatc caagattgct tcaagcggct tgcggacaaa 6600 tcccttatcc gttacaccat aggttactgt cgccattcca caacctctga gatattgcca 6660 taatcagaaa tggctacaaa gttaattgtt aagattcggg ctttcctatc tgcatcaata 6720 tctatcgaaa gaagttgacg cacatggttg gtggagcgaa ttatatttgc tacagcaact 6780 tgaatatcag aatcccgaac atctttagta aatattactt cataccaagg aagtccgaga 6840 gtgctgtctt gttg ccattc gccaagcaat gtcagcaacc ggcatttaac caattgtgcg 6900 acatattcaa ccccaccaac gcgagttgtt ccacgaccaa ttatgatatc gtggtttgca 6960 tctagcttta ggtttccgga catttatcca ttcttcattg ggccgacgtt tccgccttcc 70 20 gggttagtgt ggttatgacc atctactttg attccattca gagaataaga accaagatgc 7080 tcaacattac cggttacttt gaaggtttga cctgtaccac cggagattga tgctccacct 7140 tgaacggtga gggatttggt gactgttgtg tcgccatcca atgtaatctt cggtgcctta 7200 acaatcgctt ctgtcggaga gtcgagctca atcgtaccat ccttattaac aatgattttt 7260 gctgaattgg cgacaatgtt aatgccacca ct ttcattca aagttacacg ctgacttcta 7320 tcagcatttc gaaactcagc aacaccatta ttaaaagctt ttattgcttt cggaataggt 7380 tggttcccga cagtacatga cgccgcgttg attgaataca actggctaaa tgcaggagca 7440 ggttttccat tta ttaatcc tgcactgtct ttgccttcga cttgccagtg tgcaattcct 7500 cgctgagcga aatgaaccca cactggcgta ccgtcaggaa ccggatgcgt taaactccaa 7560 ccaccacctt gcgtgaattg aaccgttaca ttctttaatc tgctttttgg aacaagagca 7620 tatggctgtt gcattccgat aaacaaattt tcaattgcaa gttgcacttc acaggtttgt 7680 gtgtcggcat caaagttgta agcaaatccc ggataacctg tatttatatc tggagcacga 7740 gtgttatttt gactacccac gttcaagcac cgattcgtca ataatgatta agtcgagatt 7800 tctccaactc gtcacttcgc ttccgggata cgcatgattg ttcgcataaa gatttggaag 7860 tggtgtcgag tatt gtttta aaatatcaac accacatctg agaggtatgc catagatttt 7920 atccgtggaa gaccatgcca aatcaaaaca ataacaatta agaaaagtat tgagatacag a gataggttg agacaattta 8160 gcaagcttgt ccttcaaaac accgagctgt gcagttgggt tcgacaagga accaagtccc 8220 ggtaaaggaa ctccaccaag tacaccaaca ccaagccctg ttgcaacttt ggcaaactca 8280 gaataatcgt aggagttgtc gattgcagta gccgca gctt tagcaatgtc atcctacca 8340 attgtttgca actcttccag cgtaatctca accgccagca ttgaggcagt gagcttgtca 8400 tgcttggttc gtatttctgt tattacacag ttcaaatact ttccaagaat ggttgatata 8460 tacaacttgg tgccggaagt ccttaaccca ttaaataaat tgtaggtgga ttcaattctg 8520 ttttctgttt caaaagcaga agataccaca ccatacaatc caccaatttg agggaggatt 8580 ggattgttga agattgcacc actaacca gataaacctt gagcagacac agcccacatt 8640 gcagagttct gcatgtttgt tgcaactgct tcaagtttga gaatgcggtt atgattgatg 8700 atgtgttcag acacaacaa cccggtagac acaggaacct ttgtcacc gt tgattgagca 8760 agatgagact cactaaccat cgcatcaaat ttaaatgaat taaactgact tgggtctggt 8820 gtagatgtta cgatgttctc cagcttggtt ctggcacccg aaatagcttc gccaatagaa 8880 ccaagaatag aatcatcttt atcctgaact tgagttgcag gaccagccgc ccacatgatg 8940 attgatggat gcggtccgta actgaacttg ctttttaatt tggatgcagc accgctcaaa 90 00 gctgtgccaa catcaccaat tatatctgac attagaacct ctagtgtgct gacgccatgc 9060 cacttccaat gtaccagttc tgccagtttt gctctgacaa acctgatgca gtgtttccac 9120 cgagaacacc atgaattatg gtttggaagt cttggtcatg tgtgaaacct ct gtgtatca 9180 ccgttatgat ttgatacttg tcttccattg cgtactggaa taaagagtca acacggttca 9240 gtgcctgacc acgaccggcc acacttgtta caccatttgc cagcaattcc gttccgagta 9300 atggagaaac atccataacc attccgggct gaatatcgct tcgaagatac attgccaaat 9360 cgaaaatacc aatgcccgct attggagttc caataactga gttggcatca attttaactg 9420 gctctctatc tttag ccatt cgcttcatgg catcagaatc accatacgtg tctgagaata 9480 tttgaatctc agatgtggcg atatagaaat ttaaattgta ttcttcgagc aaattaccaa 9540 attcttttat gaaggtatca tggaatacac gtcccatagg aagttttgtt gtgagtgttg 9600 ttcc agaacac accaaaagag gaaacggtat tatatccata atcagtacac atagacttga 9660 ttgcatcgtt caatgttgaa cccgcaggaa tatcccgcat ttgcttaaac gatgtagcaa 9720 ctgcacctgc gtttgataca cagaataaag ttgttatatg ctcgggagct ttcttatacc 9780 ccacagcatt tgacagaatg ccagagaaca acgttggaag attttattc tcttctattg 9840 tcaccagagc attttgccct tgtgcaaccc attgttgttt cacacttgag ccttgcaact 9900 catcaagcca tccagcacgt atttcaatca agaggtcgcc aaaggtttta ttctgcaaga 9960 atttaatttc tggcaaagac aagttcttga ttgttactgt tgctgtatca gcagcccaac 10 020 ctaaagtcga acgcacctca aattcaatac tgtgtttttc aaatacagtt tttattttg 10080 gattctgtgc gtttgtaacg ataacctgta cttgtctaat ccacacaatg acaccttcat 10140 aaatcaattt caaataaagt ttaagaaagc gcggagccga agccccgcac aaatctcatc 10200 gacgctgtgc aacagaaccg tgatttgagt tgattgtaac aacttcacca ctgtacgggt 10260 tagtaacaga agcacgagtg cttt atatt gcttg attgcactat caacagttcc ttcgccatcg gtgtatgcag 10500 cattagcttt acgccaatcg ccaccgtaac gtttcatcat tgcatctaac ttctcacgac 10560 caacacggtt atattcttct ggagaatcat ttgccgctgg acgaatgccg taacctgggt 10620 cacgggctgt agaaggaaga acctgcattg agtacttcgc accagacgat gaagtaactg 10680 gattaccatg agcatcgaag tcttgaccac ctgattccag at tgcgaacc aagtcaatca 10740 tgtctccgcc agcacggaag gcacgttcag cacccgccgc agcctgcacc atacttccag 10800 ataaactctc accagcacca cccaaacttt cgaatgaata acgaggaaca gaataatctg 10860 tggaagcaag agcacgattg cgttgg cat tttcccaatt ggaatttgca ccagaaacat 10920 cttcagcaga acgagcatca accaggcgac gagattggtc catcgttgct tcacttctgg 10980 tagcaacacg agccatacca tcaaggccag ccatttccat agcgccagca attcgagact 11040 gactccagcc acgttcttcg ccacgttgac gaataagtgc agctatcttg gttgcatcac 11100 caccagaact ttgaaggtcg gcaggagtaa tcaatccacg ggtttgcact gcaatttgaa 11160 cagcagcatc agggtctcca ttttgcatac ggttgtatac agaatgaatt gtttgattta 11220 ttgcaccggc ttgatttgca ttcaagccca tcatttccaa accctgagtc tgtgctgcaa 11280 atgcaccaca agaagt catg cctgaatctt ctgcaatacc acggtagttt gacattgttg 11340 caatgccttc accaccagct tgcatagctc caatagttcc tcgaagaatt ccgccaacca 11400 tcggcagact ttcgattgca tctaatgtgc cattttgcaa cccacgcaaa ttgccgctac 11460 ggaggccacc atagagcgag ccgccaaagt catccatcca cccacttgca ccgccagagc 11520 cgccaccgtt gccgcctgcg ccgcctgagc caccac caaa cattcgttgg aagaatccac 11580 gaataccacc ggagccagtt gagtcggcaa aatcggcgtc tggcatatca gagccaagca 11640 agccagcaac accgccagag gctgcaccag cctgagcgtg gcgctgtttt agctcctcct 11700 gagcacctag tatgccttct ttgacggcaa gcgccatgcc cgtctcagcc ccatttccgc 11760 ccactggcac aaggccatta ccggcactgg tcacaaagtt cggaatatag tttgaagacg 11820 gttgagcagc gtcattgtag gttgaaccgc cgcgcccaat gaatgggtca gcgtcagtac 11880 cagcagttgc agagaaacca cgattggaac gttgagaaac atcacgccag atgccttcgc 11940 cctgcattac agaacgagca cctttgatta acgcttcttg gtcaaggtct ggattgctt g 12000 caacaaattc acgaccggca gcaagacgac cacgaataga atcgagtcga gttttgtttc 12060 tgccatacca acttgggtct tggtcaatcc attggtcgcg agtttcactt ccaattgtct 12120 gcgagaataa agttcggttc cttccagaaa ccacacatgcc cat ttgaatc tggtccatgt 12180 agtctggatg gtcacctgca agcaaatctg caaacttgcc agcacgttca ggagctttat 12240 gctcccacaa tgcgtcatcg ccaattaaac catctggaga atacatcatt cccggatact 12300 ggtcattggt gattgagcca acttgttgga tttcttgacc taatctttct gagacacgaa 12360 tacgagcttc atcttctcgc ttgtggccca tcgcaaacat tctttctgtg aatgcgttct 12420 tacttccatc accgcgctgc aatccaatgc ggtcaatcat ttctgcccaa ggtcgagtat 12480 atgaattact acccaacatc gagccaacag tggaaccagt tatgtcatat tgactacgga 12540 agtcgtgcca ttcctgagaa ccttgttgga aattatggaa tcgaacagtt gaaccacttg 12600 gaccaccatc agcaacatca cggaagtttg catgagcagc atccaagttt gcaatggcag 12660 cactttcacg acgaacacga gcagcattcc agtcggttgg ttcatttaaa tcagcagacc 12720 atgcagtgcc aatctcagaa cgacgtggca ttcttaaatc aggattggca acaccagatg 12780 gagcttgtgc agcttgagca tccatgcggt catagttgtt ttcaattatt gctgcaatgt 12840 tatttctgt ag cgtactac 13020 ttacttcact tcggattgct tttgacgctt catcctgtcc ttccgcacgt cccagcagag 13080 aagcgacaag agaataatcg ccacgaccac taccaacagc agagcccatt gagccaagag 13140 gcaaaccagc agcattgaga gcttcgcgag gatgttgaac accacgcaca atatcagaac 13200 caagctgagg caagatggaa tgatacccaa cctttgcacc agtggcagca gcataaccaa 13260 cttcaaggaa ttgacctccc atc gccgaag ccaaacgcgt tgcaacaagg gcagcatctt 13320 tctcatatgc gtgtggattg ttgtaagcag ctttgtttaa atacatggat gaaaggtggt 13380 tgtacgaatc gccaacatgg ccttattcg tagcatactc acccataata tcaccagcat 13440 aatgcccagc a agtggtggc ataatgtctt ggtaatcatc ccaatggtta tttaaataac 13500 cttttaataa attgaaatct tgcgcaggta attcaggatt tggttttggc atatgcagcc 13560 aaacatcatc ggctgaaaca ccaagttttg ccatgtagcc atgaacagct tctacatgat 13620 gagacttacc accagacata atatgatgga aagtttcggt atatcctgca ccagcta atg 13680 actgtgcaac acgcgctgca tcggaaatgg attcttcttc ataaccggtg aagccttgca 13740 tgtggtcaaa gccaccttct gcacttgcgt gtagtaattg gctattgacg tccatccaac 13800 cggaagcctg atgctgttcc agctgtaaaa cc attttgga cttcgagagt ccgggctcaa 13860 aattcaatcc ggcctcaaga ccaagagcgc ggagaacatc cacgcccatg cccatcattt 13920 cttttctgct agccattact gttcccgctc aactggagga tttaatttaa ctcttaaatc 13980 taaaatgtcg tggaatagga gcaggtcgat taatgaatag gttccatctt gcaactcgtg 14040 gagtctgcat aaagaactgt cttcaataat tgggcgatgc agaaaatacc gaatgtccgg 1 4100 gaattgactg attaagtcgt cggagtcgta ttcttctggc tcgcttccat agccaaccgt 14160 tccgcttcca aacgctccgc ctccgccaat ttgaactgtt cgttcatttc gagccctagc 14220 ttgaaaaaag ctttatagtt tgcttcaagc acgaaggcaa atacttgaca agctaacatg 14280 agctcgccat tgaaatggat atcgaaagtc gccatagtga ttggctggcc ttcaatgttt 14340 gcattacaga ttacacgctt catcaagtca gtaacaactt tgttatctgc ctgacgcatt 14400 actgcaatga tgtcaccgaa attgtagttg ttgtcaatga gtgggaacac agtggttccc 14460 actttaccaa tgagttcaac atgcaagtcc agtgcactac tcgccgccag ttggcgggtc 14520 gtgaagagtt tggtaactcc ttccgaagtc ttaaactctc gcgtgagtaa ttcacaagcc 14580 attctgtttc tctcttatag cttaaacgcc aatctctgga taagaaccag tcttaatctg 14640 gagacgttcg acgtagattg cccacaggtt tgaggtaaag gaaataccac gaac gattgc 14700 aggctgttgg aggattacac cattgacgcc gttaacaagc atgtcgccca tctttcgac 14760 aacctgaatc tgaattggtt gccacagaga tttattgcca gacaaaccag ttgcctgagt 14820 caattgagca cgctggtaca gaatctcatt ccaatccgcg gtttgcagga gtgggaatgt 14880 aatcacacca gacaagtcag cagttgtcgc aacagcaagt ttgccatatg cgtccattac 14940 tgga agatgc tgaggtacat ttcgacgtgc agtgataact gcctgaccag cggtgaaccc 15000 gtcaacacgg gcaccatcaa tcagcaggtc agtattatag aatgaatatt gcttcactta 15060 gcacatccttgt actacgaa gttgccagag ataaccgcac cttgcagcgc accagaacca 15120 atcgcaataa aggaagaacc ttcccaaata cgtttgcctt tctgagattg caacagagcc 15180 aaatcggttg agaaaacttt gtagccgtct ggatagaaca cgccttcgtt gtcgtaacct 15240 ggggcgatta aaccattggt cacagccagc tcaagagcca gagtgatttg ctggttaacc 15300 attgctacgc cggtttcagt ccaaggaacc ttggttgttg actgatagaa caggttgaac 15360 acattcacct gaacttt ctg agtcatccaa tcgacgccct gaattgtatc aaaccaagtg 15420 ccatctgcca tcgcaccatt gtagaacatg gtgtttccgc caacat gaatgcgttg 15480 ccgttgtaag actgcaatgc agcaagttca gttggttcaa ggttagcagt ggtgattgat 15540 ggaccttgtt tgaatgccag aatcagtgca gagttggcaa cgttgaagtt gacagttgca 15600 gcacgaccga ggattgaaat ttctggatat tccacgccgc catttgaggc atcatacacg 15660 cagattgtcc ggctaaggtt ttgagactta gctttgaaga aatagttggt agtgtcacca 15720 gcggtcagaa cttttggaga ggcagaagcc agaccaaata ctttaccagc agattcacac 15780 catgcagcag catccagcag aggttgagtt tcacgaaga g acttatctac agcaacatag 15840 tagaagaact gttttgtctt ggcagcagct tgtgaagctt tcgcaaggtc attcgtgata 15900 ccggttgaag caacacccgg agttgaagtt gcaccagtgg cagtcgtcag tttcagagct 15960 gttgacaacg gagttgca gc aggagttgaa acagccgaag caatgccaga ggtgattgat 16020 gtaattgtga atacaccagc cgcctgagtt acagcaacac ctgttaacgc accttcaact 16080 aaagttgcaa cagcagccaa atcggcagca ccagacaaat caagagcgga gattgtctga 16140 ggggcgccat caatgttcag ggtcattcca cccgcagtga ttgctttcaa atctgccaga 16200 acagcagcac cgccaccaac aagtttgccg gaggt tgctg gagaaccgga agttgtactg 16260 atagagccaa cacagaaagt tttaggtgta ggcttctggc tgtaccaagc agttgcagct 16320 ttctggattt cacctgatgg gaaatcttca tccaaagctt ttttgctggt atagatgcgc 16380 agagcatt ct cacccattac aggttgaaac tctttcgtca taaagacgat tggaccgtaa 16440 ccatcagtcg ccactgcgtt tggtgcaacc gcaatgctaa cattgatgat atcggaaatt 16500 ggaatggaca tttgttatcc tttaatcgtg atgacttggt caccatcaac aaacttacca 16560 ccaacaactg catcggacat ggttccaatc tcggaaagtt gttcacgaag aacattgaat 16620 tgcattttaa tgcctttcct tacttcccag tttgtttcca gt tgaacagt tgcgaggttc 16680 agcgcctctt tgcctaatgc tgcgaaacca tgttcgcgca atttagcatt aacatcaggt 16740 cgataaaatg aattatcgaa atcaatatac tcctgaccgt cccgactaaa gaggatataa 16800 aatgtcagga ttc taatacc acgagttcga aacttctcaa caccattgtc atcaatgagg 16860 cgagtttcat cgtagcccgg attagaactt gaaatacatt tgaccgcagc atattgacct 16920 tccggccttg gtgcattcaa ttgcatttcg tatgtgaaca atggaatacc cacacacaca 16980 tcgacgattt ctttcatcgc ctgaatttcg cgctcttgaa tatccattat ttctccagaa 17040 cttttgcagc gattacgaca ttgaatcctg catcatcgta cttatcaatt tcaacc gtgtaca gtgtaca acg aaacttctta ggtggggcat 17280 atccttcccc aacccaatta ttctttccgt cgtaatgccc aaccatgtaa agttcgatgg 17340 tgtgttttgc agttgtaaaa cgattaaacg ccagcttttg attaatcatg cgaacctcgg 17400 accagcaaat gccatccgac gccaacgcac ataatcttga ccaaacgccg ttgagaatag 17460 gtctgcttcc tgataaggaa ctttgtccca aatacggtca gcaaactcca cctgaacatc 17520 atc tacatct gttcgaacga ctggcccagc agacatcccg cctgaatctt catccaaagt 17580 atcacccaac ccaaactggg ttgcacgatg cgcaattaat gcagattgg ctgcgtcata 17640 gaagttgagc cagcgtccat catccgtacc catgattagc acggaatctt cttgcaggat 17700 ggcgaatctg tcatccgtca aagacgccat cgcaggatag cgccgaacta aatcagtgag 17760 tgttaacatt gattacgcca gtttgtcgta cagagcatca atctgctcgt cgctcaaatc 17820 tttaacggaa acaccgttct gattcaagac agcgagtttc tgctctttgg aaattgacgg 17880 acgctctaca acagtgaagt cgcctttctt aatctgctct ttgaacaggt taacaatttt 17940 aacttcgccg gt ttcgtaga actctttgat gaggacattc tttttgtcca tcttaacttc 18000 cgcttcgcct tcaatttcta cttcaaccat gcgttgttct ttgatggttg tcagaggttt 18060 gcagagagct tcgaagactt tgtcatccaa ctcaacagtt gcaccacccgg gaatgtgaat 18120 gaagtcaaca tccggttgac catttttctt aaccggatag aacaactgca cattaccgct 18180 gaggttgttt ttaagttgca tctttttctc ttaattcgaat taaacaaagg cgggatattt 18240 cacccgccat tcaccaatca gatacctgag ttgatggaga ttgcagcagg gtacattacc 18300 tggaaaccag cgaaacgacc gcgacctggg atttcatata ccagaccgtg caactgcacc 18360 ggctgccaag taagagccag tgg ctcacgc agacggaagg ttttcaggcc attaacctga 18420 cggcatacaa caacgaagca atccgcacca gaagcaccgt gacctttgat tgcgttcagc 18480 gctttcactt tctctttaga gttaatgaat tcgttgttcg caatgaagaa ctgcaagatg 18540 gtcatgtctg attgctcaga acgtgcagtg ttcatgatgt actgatgatg cagaaccggc 18600 atccaaactt cgtcaggacg catgattttc agagttgagc tgtacattgc gctcaccgct 18660 gtggtcaggt ctttcagaat ttcggttgga gtttgccat cgaaccagcc agttttgctg 18720 tttgcgccag cagcaacagt tgctttggta acatctggat tattgaagaa accaatgaaa 18780 ccattggcat catcgccata ccatgca gca gagttaatgt actgctcgta accacgagta 18840 gatgccattg ctttacgcgc ttccagcggc aggccagtta cggcagcaga tgcgatttcg 18900 tcaatgtcgt aatcgtatgc agtaccaact gatttcaccg gaatggaata ctctttacca 18960 gagatatttg att tcggcag gtcagttgca cgagcattga ttacttgcgc tttaccaaca 19020 tggttgtagc tgcgataggt cagagtctga acaccagcgc cgccagttga atcaacaccc 19080 agcgcatcac gagcttcaag gtcagggtac agagtgtcgt aagtttgagc ttcaatgaac 19140 tcaagttgac gctggaagaa cacagcatcg tcatcgctca gtgttactga ggcgttagac 19200 tggatagcaa ccagtgcatc atccagttcg aattctgaac catcgttcag tttaatcagc 19260 tttggcatct attcaatcca attatgcagc caagttattt ggccgcaatt aattcaggtt 19320 acggcttgac cgcaacacct ttcaaagtaa cagtacctgc tttggtcaca ccactcgcaa 19380 gagtgaaggc cagagca atg tcgccagcag taacatcatc agcgatagtc agcgttttgg 19440 ctgtgttatc ccaagttat ttggtgccgg ttacagcagt tacatcagct ttagttgcac 19500 cattcgaata atcgaacaga gccgtgtcag cgattacatc cccgcctttc ttacctgtaa 19560 catcaccttt accggttacc gttttaggta agaccggggc tgctactttg ggagcgcgac 19620 aacgatgtga actggaatca catcaccagc gttggctgga tagctcaggg ctttgatgtt 19680 agttgctttg gtgtaaccag cagcaacgcc gccaaattca ccagttgcgc taacgccaat 19740 atccgcagaa gtgattgcag ttttcagcac gatgttgatt gaaccttcca tcattacagc 19800 tagtggttga ccaactggaa tgttgatggt gcc gtcgccc ggacgtgtct tagattccag 19860 cacgttctga cgcatggtaa tacccaacac ttcgccggta acatggccga ctttgatggt 19920 gttggatttc gcaccctgtt gaactgccag accatatgca gcttgtgcat cttcggtcga 19980 gaaggtcaga cgggttgagt tggtgaaagc taaaccatac acgtcaccag cagcagcagg 20040 cagtgtatta atgttaaagt cttgctttgg cattgactga atcctattta ttaaaacgag 20100 cttggcgacg agcaagagct tcttcgcgtg ggcttagttt cttctcagct tgttcctgag 20160 aaccgtgctt gatagaaacg gagagtgcat cagagagagt gacttcatca ctatcttcga 20220 gcgccatttc gaaacgacca ttaacgtatt cgtcagattt ctctgacata tcggcatcgc 20280 gaagtttcgc aacaaccata cgtttcatat ccatttcggt cttgccagtg aagtccatag 20340 acgcatattc atcaccgagt cga aagaagt cttgtctaca atttcaatct cttcaccgga atcaccgatg cgagttgttt 20580 gagcacgacc acgcacaaca attgcagcat gattcggaac gattttgact ttatcgaagt 20640 caacaccttc gtccgtggag gcttccagca aagcgttgg gcccaatgat aattggtcaa 20700 cacccgaatc aactaagcga atcgcagatt cgtcattgag gacaattgat gcagccaaga 20760 aagaaccatc agcaaccgga cgaccttcaa tgaagccctt ctgcaattcc ttattgttct 20820 taatgcttac atcttgtttc ggatgaccaa tagtgattgg aatcgaacgg caaccttcaa 20880 tggtcgcttc atcgaacaac acttctggct tcgtgcgaac acgacagatt tggtctggtg 20940 gcagatgtg c agcaacttca cccagctctt tggctttata gagcataatg ccagtgcgag 21000 caatagtgca ttccgcaatc atttcaccag aatcgcgcag acgacgagtt gttaaagttg 21060 ctttgtcggt cagaagaacg acaccgcctt caacagtttc ttcgtcgttc agtt gcacga 21120 attggttttg cttaatttgc attcccacta ccttttggcg gagttggagg ttgagccggt 21180 ggagggtcaa ttgggcacgc accaaataca tggctgtgtt ccagaatctt gttggcattc 21240 tcacgagtga tggtgccaga ttcaatcaat ttctccaacg caatggccaa atcagatgca 21300 cgttttgttt tctccgcgct tgactcaggg aagatatcac agaattcata agtgaattct 21360 tcaatcccaa agtggg caca tgcaatcgtg tcgagaatct caagacgagg ttcgaagatt 21420 gcagtctgta aaccagtaag caagtcaata tagtttacaa ggtcggattc accagtagca 21480 ttcatgccgt ctggcgatgc tgaaagaaaa cgtgttgctg ggatacctac cgctgccgca 215 40 acgattttca agtattccca aattaaatct tttacgcctg acaaagcgat agacttggtg 21600 gagtattctt ccgactcgtc aagaataagt acgttgtaca aactcttggc ttgcttcatg 21660 agtcggaatc gtttcattac tgaacgctca ccttctggat ttgtcagaag agattgcagt 21720 cctgcgaccg tcaccacatc aatggttgct tcctgtgcca attgtgcggc agcagaagca 21780 gtggtgtaaaggtcgat ggtgctcatc agtggtatca acactgaatc cgagtaccac 21840 atgtttcttt ggaattcgaa caacggcagt tcggttcctt cgaaacgtaa gaaacgacta 21900 tgatgaatca tcccagggta tccgctcaga gtgtaatact ctggcaaacc gtaatgaggt 2196 0 gaaagtgcat caagtacaat accaccagcc gcatgtagtc gcgaacggtc aataattttc 22020 agggatttga cacatccctt cttcaaatta tcaagacgta atggagtatc aagtctgccc 22080 gtacctttga ggtcgagaag cataaatgat gttccgtaca atcttgccca ttggtagcct 22140 tcacggcaca gcttacgcag tttcaatttc ttgtctgcca actgtccttc tggcgtacta 22200 attttgcgcc aagttcgcgt catgtcttga g gaataatgg tgcagacttt ttgactaacc 22260 caatcttcgc ggaagcgatt catgagctga cggtagtctg catttttgtt ggagaaagtc 22320 caccgattat aaaccgattt atctttgtct ccacccatac cacttatgac attctcaaga 22380 ccgtcaccga ctttaatc ga gcccttgggc cgattctttt tggaagacat tatttcttcc 22440 ttttgagttt aaccccgttg cttttcacga tttctactgc ctcgcttgag ctaatacgga 22500 gttcagtttc agtgcccaaa gtttcgacaa tcaccagcgt tttgccagtt actgcgtctc 22560 gaatttcgaa accagtattg tttttgcttc gggcatcaat gcgagtaatc acactcattt 22620 caaacccacg cagagtagtt ggcgttgttg ccggagaatt caattgcgac catgtctgag 22680 acgttatcgc aacaatcgtc gtgtccagta cctgaaccca aagaagtcat cccaagtatt 22740 tcacgtttaa catgttcaag atgttcatgt ccagcaggat aataaatctt gccagttgag 22800 aaatatggaa tggtgttaag gaatcgtgct accttatcac cgccagactt gtcacgagga 22860 acaggcataa gcctgatttg accatcacga acaaattgct ggtttaggaa ttgaccagat 22920 gatttgtctt ccatgtacaa tgcacgggga atacaacaag gccatcttga atcaaagata 22980 ttacacttct tccagaactc gtttattgcc agcttcaact caggaacttc atatttccca 23040 agcataatat ccaagcaata tacatcacgt tctttcgtga cgccccaatg tttcaacact 23100 gagtag tctg aatattcttt cgcagtagat gcggtatcag cagtgatgaa tgaacggaca 23160 atacggctcc ggtcgaatgt ttcaggattg tattccttcc accattcttc tttgaccaga 23220 cctgtaccat ttgcagttgg gtcgccagca tactggctgt tgtatgtgta tggattc gct 23280 ttctgcattg cttccagaga ttccaaactt ttacgagaag gccacaaagc agacttttcc 23340 tcagtacgtt tcaaatcata gaggattgga atcgcatgag tgtatgcctg tttcttaata 23400 atctttcgt aatatgcagc agagccagtt tctttctcaa cgattgctgg aatgttgagg 23460 taatgatatt tatcagcaga gccaccacga agcaagtaac caacaaggtc actgtcgtga 23520 acacg at ctactaca 23700 aagcaaccgg catagttctc agccaacagg ccagcacctt taccagtcat tttaccgccc 23760 gttggaacag cgtgcatcac accctgcttc gtagtacccc aacgctcaag agaacgtttg 23820 tttgggtcga tgcgaacatt tgggaagatg cgctggaaca atgggtcaag cattacagtt 23880 cggatatagc cactggattc caatacaacg tcttccgcat aggaagtaat gatgttgtgg 23940 ctcgtcacgt tatgacagaa tgaatacaac ggcaatgcaa tggatagaag ttgagtcttc 24000 gaatgtcgag gcgggattgt gataatcaca cggtcgattt taccatcaac tatttgttga 24060 cctacatcga agataacatc atggaagtct tgagactgaa atgggaatcc catttggata 24120 tgaaatga cc atttgccaaa cttctcaaaa cttgaagtta atatttttct gacttcatct 24180 gctacctttt tcggtacaac agttgggtct acgttgagtg ggtcagtcag aatttgctgg 24240 atgagttcgt cggttgcaga gtgttcgtca gtgtcggctt caattgttga gccagcaatg 24300 cggtctgtct taccgtcttt aatcatcttt tcgatttcac tggcatacgc agtggaatct 24360 ttagagtcgg ctgcttcca a cctttctctt tgtgctgtaa ccaaagagcg caggacagcg 24420 ttatctttta agccctgtcc ttcaatctct gcatccagtt gctcagagta atctctgata 24480 atgtctggat tcgccgcgat atacttacga aggattggca gtgactgcat acaaaagcgt 24540 gc acggctaa cttttaataa gtcgccgtag aacttcaaca gatgatagac aaccgtgctg 24600 ttgtattcat cacctaaatc gttatccatt tgtttgcctt tctggtaatc gcttagtggc 24660 gtctattcta ataaccttct ctaaatcagg ttcaattctt ttttgcaggc gattcaattc 24720 catgaatgaa tcacacactg gacattgcat taaggagaag tcatcttcac caatagcata 24780 gccacattcc ttacatgtga tagtgcgcat aagcgtactc caaga agata ggaggccaaa 24840 gagacctccc aaccatcggc gtgcgcttag tatttaatat cgttccacag catatcttcg 24900 aaatgttctt cgagtgcttt ctgattctcg ctaattacag ctacaatttt gaaaggcaag 24960 ataagcggct tcaccgctga cattaaatct tc acggccac gcttgatacg ctcaatgttc 25020 cgcagtgcat tctcagagtt cggaatcata aacacaatta catcagcgcc agttttatta 25080 cgggcatcta acgcacgagt gatataagtt ccgccaccgg ctgaggtgac tgttgaacgg 25140 aggtaaacaa agtttacgcc gacattattt tttacttgcg ggttgcgcag gtacactttc 25200 ttttgtggct ggtttagcat cttgaacggg tt tccatgag gttacaattt tggtttcagt 25260 aacctgctca actcgatacg cgcagtcaac catactccat tcaatgcccc agctgtagtc 25320 tgacgaggca tccatagccc aagtaccaac taaatcagcg gcagcaatcg gaagttggaa 25380 ttcatccacc atat caattg caacttctaa attaacttca acacctaaag taacattgct 25440 tgagctatgt tttccaggag tcgcgtcaat ttcaaaagta tcgtcaaaca ggtatttcag 25500 gaacttgatg aagtcaccac caacaagtcc tgttgaggta tggttcagat tattcatcgt 25560 catcatcctc gaagcagtcg ccgtatccgc cccagttatc aacaccacct gcttccagtg 25620 catctaactt ttcgagacga acatcagctt ctttgatttg gtccagaagt tccagagcca 25680 tatcattctc ttctgcattt aaatgacaca gaagactttc aaatttgagt tgtaaacaca 25740 tgctcggatt ctccattgag caactacgcg cgaccggttg agccgaagcc accttcaccg 25800 cgtacagttg aggtcagatt ttcctgc act tcgaatgttg cctgaatagc aggaacaatc 25860 atcatttgtg caacacggtc aagtggttca acatggaaga cagcgctgcc atcgttggtc 25920 aacttgactt tgatttcgcc acgataatct gagtcgataa caccgacaca gtttgccaga 25980 cgaacatcgt tcttgaagcc gtggccacta cgtgagtaaa tcagtgcaac gtaaccttcg 26040 ggaatttctg gcttaaggcc agtgccaaca atggcagtat caccgggata aataattgca 26 100 ccttccacag aacgaatatc agcagcagca gcgccgcgac tttcatacga tggaagttga 26160 gccagcggat gagcaagcag gaaattgacg tgagggactt tattgggaca cattacgcag 26220 agactctctg aatgatttaa tggctgattc cacaatgtga acgcca atat taatttggtc 26280 gtctgctaaa tccacatcaa tgcttttacg ctctggtgta tctggcagtt cgacgtatga 26340 acgagaatcg cctttgaaat caaagccgtc tcgatgaaga cgaataacaa gcacgccgtc 26400 aaagcaggtg tcaatgcagc gaagttcgtc tgcaaaacca ccatcgggat agatgacatt 26460 aacatcgcca tccatatgcc cgcagagtgt ttccacacga ttaacttcaa ctttgccaa a 26520 gtaatcgctg ccataacggg gcttatagat ttcttcgctg acatagatga gcgcttcacg 26580 aggagttcgt ccgcccaatc ccgccaagga tttcgaatct ttaagtacac ggtcagaggc 26640 gaagtggatg aacttatcca aatcaacctc aaagt gctta gcagtgtgcg catatagtgc 26700 atccttaaac tgatgtttac ggaacacgcc ctgctcgaca atcttattgg ctatggtatc 26760 tttgccacag cccggtgggc catttaagac cacaccgagt ttgattctct gcatcagtcg 26820 agactctctt ctgacttatc agtgcgtaac cgtaccaaag atgggaagcg gaatttacca 26880 gcttccgtta cttcgcggta acgaacctga atgattttac caatcagttc gtgacgatgt 26940 tcccacaacc attt gcgaat gttgtaatcg ccaacaatgt tgtggtcttg aagacagtca 27000 aaaaccattg agccagcaga accgtagttc gttgcgattg agccacatac acctttgttc 27060 ttattagtgc cttctttgaa gccagtaatc ttaacatcgg catatttata tggtacaatc 27120 ttccaccaaa gggtttttcc tttctttcc ttgcaacgaa cgatgaggcc ttcatgacca 27180 agtttcagca ttttcagcat caactcatga agcttttcat tgcttgggtc ttgagcccat 27240 ccgaggtaaa gacgtttgtc gatgttgcca tcagacagtt cgtacacgtt ttcctgcgtt 27300 aacggcgacg ctggtggagc atcgctgccc aagatgcttg aggtcgtatt ccatgaatcg 27360 cggaagattt cag catcttt gaaagataaa tggtcaagat ggggtaaagg cttggaattg 27420 cgactccaaa ctcgtccgtc tctattccta atcgcccgaa tcccatcaat cttgatgtaa 27480 atttcgcaac gacctttgag ttggcaattt gggtcttccc aatcatgggc atgtgccttc 27540 gtataagatg ttccgtcgtc caataactta atagtcattg cgggagtacc ttcttaagat 27600 attctcgaac cgcattcttg cgcccgattt tggtttttgg gactggatta aaacca gcga 27660 gttcacattt cttaatttgt tctttatcag acaatttata gaatgcgatt actttcttct 27720 caatgattgc ttctcgtttg gtaacgtcgt aacgctccat gtaagttaca atcccatgac 27780 aatcatggca aagaagttcc aagtctgatt cctgaaccaa taataaatt g tccatgaatt 27840 catgtcgctt ggataaatcc gaaaggccac cacatttgtt cttgtggttg acctcgattt 27900 ctttaagctt gaacagcttt ttgcaatgct cacactgcac atgatagatt ggcttcttac 27960 tgttgccaac cctcagtgcc actcgcttct tctcaagaag tgcaagtttt gatggatgct 28020 ttgcccaaac acttctcagt gctgattgaa gccaagctgt aaacctggct tcggttttgc 28 080 aataaccata catggcaaat tcacatccca ctaatgctca agcagaatgc cacacgaata 28140 aaatcacgcc gaaatggttt tagaattaag gtcgaaatat tccacaacct gtggattata 28200 atgccaaagt cgaaccttgt ttccttctgc caccgtattt gacatatata gcaatc ggcc 28260 gttcacgaga agttgttcct gccaaccatc accaaagcgc ataacatact gtgtagctac 28320 ggctttcaaa ccgtctgcat agttgtcaat tttgtccagc aattcgaatg cttctacagc 28380 accgacaccg acacgctttt gatattcttc accagccttt gcgccagttt tgtacatctt 28440 tgtttcacta acaccacaac ccattacacc atcggttgag tcaccagtga ggcactgcca 28500 aaggaagaac ttcggaccaa agcctttgat ttctttcttc ttggttttac cattatcttt 28560 gacgatacat ttaagttcgc cgaaacga gacaacagtg attttctcac cttgtgtgcc 28620 agtttcccaa cgataatgtt tgacaactgg accacccgga cgaccaccga ttt gtttcaa 28680 atctttgtcg agggatgcaa cccaaatttc gtcatagtca tcaagagtca atgggcgaat 28740 ggttttaatc gttttgattt caccacgttt acccattgtc atttcgactt tattacgaca 28800 cagaatacca atcaggtcat cagcttccag accatctttc ttgataccgt tataacaggt 28860 aatcaaagtt gctttggcat tcttcaaatt ctctggtttc catacgccat cacggtttgc 28920 tttgtatgta tcagcgataa ccatgcgcat gtttgaatca ccagaaatca gacaaatgtg 28980 ttcagttgcc tgacaacgct tttccatgaa ttccattttg gattccatag acatctgaat 29040 ttttgcaaaa gaaatatctt cggactcttt cccgtactct tctgccgcgc aatggctgaa 2 9100 tgccaccatg tctccatcgg tcaggaggag aactttcttc ttactcattt tgcaaaactc 29160 cagtaaaaga gggcgcaaca atcttggtta cgccctttta ccaattggta aatacatcta 29220 cccaacgtag atgcagtgtg ttacttagaa aggttgacca gcagcggcat cagcgccacc 29280 attctgttca gccagtttag ctttcgcagc agcctgcgca gcaagttcag cagccagagc 29340 ttcatcaacc tgttctggag ttgct ttgtt ctcaacaggt gcatctggag ttttggcaat 29400 gcccagcagt tcctgcaact tagaaccgtt gaagtccaag tttgccagaa tgcggttctt 29460 aacagcccac tcttggtcgc cacccggcag cttgccgaat acttcgaggt caggagcacc 2952 0 caagtcgaag aagatggttg gattgaccag agctttcgca tcacgtttgt ctttcgactt 29580 cattggcgcg aaggttgaga ttttgtttgc catctgacca gcattcttac cacctttctt 29640 ggtgtactga gtcaggttaa ctaccagtgg ttcgcccagc agttcattca actgtttggc 29700 tggatgaccg gcaataccag cttctggatt cggtttaact tcaaatgcgt caaccgctgc 29760 catgaatttg tagatgtgag aacggtcacc cataaatcca tctgg attgt aggtaaactc 29820 gaaatcaaac caacgtggtt tgtccagcag gtctttacca atcatttcgc ctggctcgcc 29880 atcagggtct tggaccataa tgatttcgcc tgcttcgttg gtttctttca tgaactcatc 29940 gagtagctcg aaagttccca g cagtttgta tgctggttct ggatactgac ctgaacccgg 30000 ttgtttaccg aggtcaatca gacgtaccag acgggctgga taaccacctg attctaacag 30060 tggtgcttta cttggtgctt taccggaatc tttggttggt gcttggaata gggccatttt 30120 gtttctccat tgaaacagtt ttgtgcaaat tagtggattg gcatgattgc cagtttgaaa 30180 tcataggatt ccaaaacaac taaaagttct ggaacatcct tt gagttgga aattgctgtg 30240 aggaaatctt tagcccaatc ttcttcgact gtcttgctaa taactttctc tgtcagatat 30300 gctgccacag catccttcgc aaggatggtg gcatctgcac taaactgaac cagcgtcatt 30360 tcctgtgcgt catacaacgc gtaa atgagt ttagccatta gtgaatctct gaatagtcgt 30420 cgccgaattg aacttcacac gcaacatcgc ggttcatctt caattgttta ttggtgcgtt 30480 ccattgcttc tacacccacg atttgttgcc acagttcacg attacctttg cgaacttgca 30540 gaatcaattc atcatggaat tgtccagaca gcattgggtc acgtccataa cgttcgttgc 30600 aaatttcaat tacagcattg caccaaacat caagacata cgcgccagta ccttgacaaa 306 60 gagttgagaa acggtctttg tcattacgca gtgaatacca gaaaccattt accggattca 30720 actgccacat ctgaccatca atcgttttca cagttgtgtt cgctgctact tctttgattg 30780 accaattcag acgccagtaa gcttgatgca gcttctcagc tgtagcccaa tcacacttcg 30840 caccacgttg cactgtcgca atacccgcac catactgaca agcatagttt gtggttttga 30900 acattggacg ttgaggaacc ttcttgattc catctttgtg tgcctgtgat tgttcttcgg 30960 taatgaatcc accgattaca ccaattgcca agtgagcatc atagtctggt gccaactgtt 31020 tcttgacata ttcagggtca tactgccact ggaagtggtg cttgcaacgg tcttcaagtg 31080 aagataggtc tgaaccaagt tgttcataat cttcatttgg tgcttccaac atgccgcgaa 31140 gttctttacc accgaacaca cgaatggatg gaatatttac cagaccacga tgtttcagtc 31200 gcagcgtgtt agtaagacca gcgcaactag caacaaggaa gccatcgtcg gatacatc ac 31260 ggagccagcc gttgacaata gacaagcggt tacgatatac accgaggcca ataagatgtt 31320 caacaccttt gccgggatta tcttccgcca gttcatgcag gctcggacaa atatctggaa 31380 taccatcatc atccttaact gtgatttgtg gaatctgacg agtttcattt gtttccttgt 31440 cacgaacaaa cttgaatgtt tgcggcttcc agccaagact gaataaccaa tcttttactt 31500 gagcatgaga cg cagcattt ggttcgtcgt aaccttttaa aacttttatg acgaggtttt 31560 tgtcctgcca atcaagacca tttgcatcac agactgcttt ccatttggcg cctgttgcag 31620 acaatgttcc attttgctta tgacacttgg ctggacgatt cttaagtgca tacttagga a 31680 cctttggcat cgacgcttcg agtgcaattt ctttctccga aatcattggc gcaagcatgt 31740 cgcgaaaagc ttctgctttc ggtacattca gcttccaccg ggtacgttgt tgaatgactt 31800 gctgacggcc tttgctcatc aggtattcga caaagcgttt cacttcttcc ggggtgcgat 31860 aaatcgctaa gagttgggaa tactgctgtt cccacaatgc catttgaata cggcagtcct 31920 gcattacacg gaag ttatat tcttcctgag tttggttttc ccaatcttca attacaggtt 31980 ttggtacacc aaattcttca ccgtattctg ccaaaccatg cctcatacga cgaggttgca 32040 ggtaccagct aatgaacata gtgtcaataa tcgttacttt ggatacatca tatccaagga 3210 0 aggctaatgc ctccttatca aaacacaaac cgttgtgcat gatgagagtt ggagccgtgt 32160 cgagaaatgc ctggattcta ctacgttcag tccactccat acaattttca actttcgtta 32220 atgcatcaat cgcacccatg ttgtgcaaac gtggatgttc ctgtttcgcc atttgctcta 32280 acaaaccagt cgtttcaatg tcactggcag acattctttc ccaaggataa atcaattttg 32340 tctccaagga tggttgagga ggtt tgtcca agcttctgtt tcacaacagt aggaggacaa 32400 gaccttagtt attggtgcgg ggttgttttc ttagtgagct gaacgatttc tttcgtttca 32460 aacaaactat cttcccaatt gcgttgaata atacgaccag aatctgattc gtaatatgtt 32520 ttga aaccat ctgtcttacc actcttacgg gctttaatac cgcgaatgat tgaacagttt 32580 gggtcaatcg cctgactgtt tctttcgaaa ccaatcatga aatgggaata ttttgatgct 32640 gcacgagaac cagtgaagtc acccttctta atacgtccac catcttcatg aggacgctga 32700 cctttcggaa ccgggttaag gtgagatagg tcgaggatac caaactgata ttgttcgctt 32760 aacttaactt ggtcggcata aagcttaccg aggaaatcat tcttct caga cgctgaaata 32820 ccttctgaca aaattgttaa gttatccaag acaaacatgt caatgtctgg accaatttga 32880 cgcaggatag ttttgatgcc ttcccatgtt tcatacgggt cttcaccttg ttgctttcta 32940 tcccaaatct ccatgttcgc aagcat ttta cgacaagttg cgcggaactt ctcttcatcg 33000 aatcgtgggt cttgtctgaa tgaaggttcc caatatggga gtccatcatg caaaccagcc 33060 attcgaagca atgtctcttg tggagtttcc tccatgaaac aagtgaacac accccaatca 33120 tgtttaacga tgttgtgttc tgagagttga cgagctagtg ttgttttacc accaccttct 33180 gaaccaccaa gtgtgataat ttcgccgcga cgttgaccga acatcatgt c tgtcaattta 33240 gaccacggag tatcaacacc cggaggaact tcatcgtcaa gttccaacat taataattct 33300 tattcacca acgctgttgg caacggacgt gcagcacgga agattaccgc atcacgagtt 33360 tgtcttaaca ttcctcgttt aaggcaatca ttggcat cgt ttgccgggag ttgtgcaacc 33420 attgcacccg gtaataaacg gcatgcttcc ttcgctgctt tcttgcccgg ttcatcattg 33480 tcaaagacga ttaccacgct ttcccagcgt tgcttaatct cttcggctac gcgagccaga 33540 catttatgaa ctgaatctga accatctgat aaggaaatga ttgcgaaatc aagttcctca 33600 tatgctgtac cacggttcaa gatgcggaga atctgacgca atgcaatcgc atcttcttca a gttgtacca 33840 tcgaattccg aatacagaag tcgaacacga gcatggctcc agtattctgg ttcaatgccg 33900 cgatggtcga gattaaacac cggacaagaa gttatctcat caagttcttc ctgaatttct 33960 tctggagttt tgacgtgaat ttcttttggg tccggtggat tgtcaccata agggttggga 34020 acgagaacag aacaggcaaa gcaataacca gaataattgc gagttccatc ctcattttcg 34080 accg catctt caagatgttc 34260 gtgaacaccg ccgctatgta gtttaccatt caaacagaca ttaactgccc atttgcctag 34320 cctcttgttg aaacttactc ctttatgtcc ggaagtgttt cgtgcattta gttttgcatt 34380 gtggtattc tgcgaagttg tgcactcacg caaattatcc aaagcattat tgagtttgtt 34440 gttatccaca tggtcaactt tctcaacaac cttcctagac ttgataaagt agttcaacct 34500 gt gttctgcc acacagtatg tagtgccgca gatgtttaga taccagtagc gatatccaga 34560 ggccaaatat ggactataca gccttgcaga tactgccgca ggatacaatc ttgctggcaa 34620 tgtcttatgc attaaccttg tcctgttaaa gctttccaag ttctaggaaa atgtt ctttc 34680 atgattacat taacttcttc tgcaaacaga cgggcctcgt actgtgcatg ttctgatgaa 34740 cgctgcttgt acaagttagc ccaaccatag aggcttcctg tccacaccca agttgtaatc 34800 ataccttgtg gaagaaccat tcgagcttgc tctggagcaa cacccaaatc aattaaacct 34860 tggtattcac gaacagccgc acgttgcacc gcagcaatga gaagtttcat ctcactggcg 34920 tagtcttcac gcatgggtt g tgcagattga gtcatcagct tatgcgggcg actaagaaca 34980 agttctggtt cgtaagtttc aatctcaatc gctacaccat ctttatagcg gcgcgagatt 35040 tcgttccatg agaacccaac ctgatgttta cccaattgac gggcaaggaa gattggagct 35100 cggca gcgca aagttacatg agcatgacgg aatggagtta catgattatg ctcgccaaga 35160 aagttgacaa gatgttcatc acgctcacta aattcgtgag actccgcacc atatgagaca 35220 cgtgcagcat tgactgttgt taaatctgtg cccatcttat cgacgagtac agctgaaatt 35280 tgtttggaca ttaatgaact tccggttcaa ctttgattgg ctcaatgtaa cgagccgcca 35340 gcattgcatc tgcaattgcg taagctgtta cc gcgagttt tgttgcatga actggttgat 35400 gataaatctc agcaccagtt tctggattta acattctttc catcgcgcga agtgcaatat 35460 catcacgaag atattctaga tttgcttcat ctgcctcagc gataagctgc gacagaactg 35520 catcattgtt gctcatgaat t ctctctcaa atgggtatta aaacgattac tcagcttatg 35580 gctctttgtt ctacactcta cgttcacggg aatgaaaata agctatcgta cactttaact 35640 gtgcagagtg tagaacaaaa agactttgat tcctaaccgg ccaagcgggaa tcacttcttg 35700 atatgggaga acatcacagg ctcgtaacac ctgtcatatt tttcatggga gaatgaagaa 35760 acccatgaca tatgaaaccc tgccttgcat atttcaatgt tg cgtctcgg aggttaaccc 35820 aatcgccagt gaggagcctg actcgttaga ctgctagaca gtctggtacg atgcaaccgc 35880 aacaaatctc accaccgcgt ttctgaattg tgttacagaa actgtccgtc tggatttgac 35940 tccagtatgc cagagcgcat gttacgt ttt gaacagacgt aaaactgtat cgaaggatat 36000 ggtggagacg gttggacttg aaccaacagc ggtagcattt cagcttgcgt ttaccaattt 36060 cgccacgtct tccgtaattt ggcaggcttt tgtttattta ccggagccca ataaccggga 36120 ttcaactttg gtgagtgtgt tctcagacac tgttttagga agtgaccacc tgaagtctgg 36180 cagcttcatt ttgttttgag tctcgttgtt taaagtcgcg ccg agacacc agtctctgcc accattattt 36420 gttgccggtt ttgataacgc aggtttccag cgagaactgc gaggcttgct ttcgttcaat 36480 tgtgtccaga gagaacaaca gtgaagactc cggttcgttt cctcggcttg ataggccaca 36540 caatttcact caggctgtgt accactcttt ggcggcacac atttcttttc ataaattaaa 36600 ttatgagaaa gaatttgtcg tttggtttct ggaggtcaata aaggaatttc cttcttcg ac 36660 aacataatca aactaaaact ggaacagctt gtatcaacaa ctctagtctc gtgtgaaacg 36720 ctgcaactcg tcgtcaacac tggtatcgct agagcgatta acagactcaa caactttctt 36780 tgcagccgtg gcttcacgga cttgtgtgtc ggcaacag ct tttgtggatt caatttctgc 36840 atccttcttt tcgacttcgg ctgtttgctc tgccttctgt ttaccagaaa gataggcaac 36900 acccaaacca actaaaaaga cgaatgcagt cgcaatccat cccttaaatt ttgcaaccat 36960 cgcaatcaga ttcatcgagg atttcctcct gattcgctaa tttctcatcg ttgcgtcgtt 37020 caacttcatc gaagtcgatt tcgtttaatt caaattcgtc catcttggcc tttaactcct 3708 0 gggcgtattt tgcaactgcc gctttaaaca ttggatgcgc ttcatcaaag tgcgggactt 37140 ttccagttgt tgtgaaaagc ctgagcactt ctgcttgaat tcttaattca aactcttgca 37200 gagaaaatcc gtgtgcttct gcttgaattc tcagttctt c attagtcatc ccaatgcctc 37260 atcgaattcg cgacgaattt tcgccagtgc gccaacagga taatttgctt tcttcacacc 37320 tttgaagatg aaagatactt ctttatgctg cgagcgtttc aataaaactt taatacattc 37380 acgaagagtt ttgttctcag gacgtttgtc tttgcgtgcc aaggcaacag caatttcgca 37440 agtcatgatg aaatcataat actccatcgc gagtgacatt ctttcgtcca tcgtgttctc 37500 catgattta a atttaaagcc gcttgctccg ggtcttagcc gtcacttcta taagagaaga 37560 cacacgcggg tgaatgtgtt gtctcgtagt agtagagtat acgtcccaca actacgtcta 37620 tcttcaatat ttcaacatac atgcgaggag tcgaacctgc cttacactct ttgtgcacaa 376 80 gaggcttacc gaagatgtat gcttcaattc tttattaaga gtaggcgcgc atagtcgcct 37740 cgtgttactt gtaacctttc ggttcttctt tagtgctcgc ggacagagga ttcgaacctc 37800 catctgagtg cgcataccac acgttctttg aagagcgact tcaaatagtt tgtgttcctc 37860 gcaaaccgtc ttaggcccat tgagactaat ccgcgtctgt taattcagat accactaaag 37920 ctctcttaat tcactttgat gt tgcacatt gctgggccat gacataacgg gcatcggcca 37980 tcctcccgca tctaatccaa tatgcaactc aaagtgcttt cgttcacctc cactctacga 38040 atactcgaac cgtaaagcgc caattctccg aagaatggag gcgacagaaa acatacatta 38100 ttaatcacag cgtaccactg cgccgaattc ggttacatgg aatcggaaga ccacggagcg 38160 agactaaatt taattaggct gcaactcgca tttcagaatc gtttgcattt attttaaatg 38220 aataaaaaca atcgctcatc aacgaaaact tctggatgac cactctaaga gtgcggtagg 38280 agttgaggtt cctatccgtc tttcagccaa ctgcaagtgc acttcctcac ggatactgag 38340 agattcactg aggtctcaag tcagttacta tttcaagtcc tgtgtgcacg gtcatcttac 38400 aaattggatt attcaaatca tccagaagcc cactctcaac tctcggcatc ggctcaaagc 38460 ctctgtgctg tggttggcag aatgggattc cgggtatttc aaatcccgac aatcatatcg 38520 ggatttgcg c tgcgtttacc agacgccagc tagatcgatt ttagaacagt ggtgcagctt 38580 cttcgccggc ttcaactgga gcctgagcaa cttctgcatc agcagcttta gccgcgtcat 38640 cttcaaccac tttcgcttct ttcggagtat cgaaaacagt gcgctcaacg gtcggacgac 38700 ctgcttcaac ccacggcagg tagaatgcca ggaagttggt cacagtgttg acctgcatgt 38760 tgcgcgaacg gtcgattgca ccaatggtgt ttacaaacgc ttcagcagcc gcttcaatct 38820 gcgcgccgtt ttccagtttg aaatcatgtg cattcgcttc tgcttcgctc aggaacagag 38880 aaccgtcgtt tgcttcgtac tgagtaaccg ttaactacttt agccattttg gcaatcctta 3894 0 tgttggatag atggacacct gagattttgt gttggtgttc gcatcaggga ataaaagttt 39000 gaaatggagc atttccacat tcagctaatg ccgaagaatt gctccaatat tacaacaaaa 39060 acaaaatcaa atttgcgcga agcgcagaac gatttctttt aataaagaat ccatttcgtc 39120 ttcggtaaat tcggttttga ttgaatcgtt gatgccctca atctgcacac agacaaannn 39180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn nnnnnnnn nnnnnnnnnn 39240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcg acctacacca taaacggtta 39300 agcggtcaag tctgtcgcgg attttgacac cagcttcatc tgcaactgca cgcagcagtc 39360 gccagcggag tgttcggttt ggaac gacaa aaccatgttt gtagaagact tcaaccatga 39420 gtttgccgag acacactgct tcaacgttcc acccacgtcc aactaattcc tgcaacatgc 39480 agagagttag aagttcgagc cggttgaaat gctgagtgcg acacagattg aaatactcac 39540 ttggagtatt ggagcgagaa aatactgctt gcttaaatgc acgttcactt tcttgtgcag 39600 ccagcatcag tagttcaggc tttatcaact tcattgcatc aaagccagca cgtgtggct t 39660 taatgacgcg gcaacattca tatggtttta atttaaccca ctcacctttt ttggaatcag 39720 gtgagaagtg ctcccaatat gttactacgt tttcttttgc aagatacacg gcgtcatgtt 39780 cctgcaacca catcttcacc gtttcaaggg caatgatgc g gtcttgctca ttttgctttg 39840 ccacttacaa tctcctgcaa gacttgaatc ttttccatgt ttgcaggatt tgtgtggtca 39900 atttctttga gcgctttctg aacttcggcg tatgtttggg tcttaccatt tgcaaagtgc 39960 ataatgattc taccaatttc gccggaaggt tcagcaccgc ttaaaagcag caactcaaga 40020 gtttgattga cacaatcact tacagctaat gcaacagcaa agttaacatc ggcttccgtg 40080 aaagttcgct gtttgcgttt agccatcagt gacctgccat gattctgtta ttagcttctt 40140 ctgcgctcat gcccattgaa accaactgca ccatacattg agcaatacgg ccttgtgcaa 40200 gtttttcaac ttcatcaaaa ccttttgcgt caacacccat gctgtgtt tc acagacaaca 40260 gtgttgccat caaaccatgc gccattgcga tagcctgagt tttctggcat tcattgttga 40320 tggtgatgcc agcaagttcg tgctcaattt tgttgagatt tttcactaca cgcaacttcg 40380 cgcgcagctt ttgtttaaga gtttgcgag ccaagtttgc tctccagttc aatgattcgg 40440 tttgccaaat cacgtacaag ttgaggaccg agtgctaaat caccacccat catttctgat 405 00 aggtcttgtt cgcattcgtc catgtcatgt gaaagctgca acacttcttg gatgtttaat 40560 ccttcgtgca gcaccttacc ggatgttaga accaatttac cgtaaatcaa aatggtgcct 40620 catttccata atacgtgttg gattcccact cacgcatata aactttgcga ctaacttctt 40680 cgcccatgta agtaattaca tcgttcaaag ttttggttgt atacccgcca cacgagcgtg 40740 tgattatttc agcaattggg tcaaaccaga aaccatcttt ggctacaccc gatga aatat 40800 tggcgttctt actaccgttg taaaaccatt taccgccaac taaaacatag tgatatacat 40860 caccatcttt cattgcgaca gatgtaactt ttgcagaatg ctttcgaatt ttacgcggca 40920 tgttaacgac cttttctttc tttaaggttt gc gcgatgtt ctttggcacg gcttgaatgg 40980 aacatggcaa ggcgttggct ttctgccgca gcatccacaa cagcttgaag gtcatcacgt 41040 tctttcgaaa ccctctcaat gtctttggcc atgttctcaa ttttctgcaa caattctttg 41100 gttgcattgt ttggaacagg tttaccgcca agatgttcac tgagtcgctg acgcaagtcc 41160 tcaagaattt cattcattgg gttgctgcta acattcttaa tttctgaat tagcttcc 41220 aaagtaataa caagtctccg acttgttgct gccaacatca tttccaacgg cgcaccagtc 41280 atccattcag agatatatcg ctgggattcc acatgcattg tgaggtcagc gttggtgata 41340 aaacctttta cgttgctcat tgattctccc aagt tttgat attgagtttc aatgttggtt 41400 gccagcaatc atcgcagcac ttcccaacct gaacccacaa accattcttt acagcttgtt 41460 tacgaactgg ataataaacc tccagcaaac caaactgacg tgtacagaaa taacatttgc 41520 tcataggaat tctctccaat taaacgcgca aatgtgactg atggttcctc attggctggc 41580 aaggaaccgc tccgaaaccc actcagcacg ggattctccg tggcgctggg cgtttgaaag 41 c gctgccgtc gcagaatcgc 41820 tatctatacc actggtttta cccaaaatta tcagccctaa cagcttggta caaatagtca 41880 cattcacccg gtcgagttta cccacatgta gtttcattat gtgcccttaa agataattat 41940 gatggctcac aatcatcaag gcaggttgaa atatgtctcg ccggtatatt gtagttacaa 42000 cttcgccgca ccaacccaac atagcgggaa taatttcgta ttctcacggt ttattgttaa 42060 aagttaatag tgca agtgaa tgatagcata acgctatctc aaaacagact ttgtgactaa 42120 cctgttttaa cgatagtttc acgccgttat tatcggtttt ggtcataacc actttttccg 42180 gtagatgtgg ctgcatctat cgtcacaatg tgtttattaa cccttgacac aattttgtat 42240 aatgtccttg acctacagag cacacgttcc acactttagt tgtttgtggg aacacaccaa 42300 cgtgccaagc tggaacacca atgttccaag tgattacaat gtgcttcgca cctccagcta 42360 atgccgaaag cgcgaagcgc tcacactgct acaaaattac cgcacgaatt aaaatccagt 42420 cagaatcaaa atattcgtct ggtcggaagt acatcttgaa cttgtccggg tcacaatcaa 42480 cttcaaacgc tgt ttgattg ccaaacaaat ccttcacgcg aacatatgtc cagccttcaa 42540 cccatccatt cgggcctttg cctgaacaac ggttgatgac ttggtaatag tgctgacggt 42600 cttggtgata aatgatttca ccaccagtcc actttgttgg ctcagcttgt tgcgaaga aa 42660 tgattgttgt cttcggagta ttctccacct gctttccaat caactgtgat tttgtcatag 42720 tcaatgtaat tctccaggaa ttctggaact tcgtctttgt agagttcgtc ccaacgttct 42780 ttgacaaatg attcccaatc cttgaactcg ccaatgtagc ggtcttgaat aagggataat 42840 ggaacatcgc aggctatcgc cgctaaaaca atttcgtcgg cataatcgct acgagcgtca 42900 ctgatttctt cgtacatttc ccaatcaaag cctttcggct gagtcaggca acgttcgcac 42960 aaaccttctg ctgcaacaac ttcccaatct gcatttgcaa ttcgttcagc aacatcttct 43020 tcaatctcac aatgagttct gaggaaggtt gccatactta cagaacccaa tggattggct 43080 tgatacaatg cttgtgcatc taaccaaata tcagttcctg acttcaaacc ataaaatcga 43140 acacaaatat cacgaacatg gtttgtcgat tcaattcttc gaatgtaact atacccgtcc 43200 ataacttgct ccgaatttaa gacgtccatg taaggacgcc gttgcattat ttctttcact 43260 gctggcctat tacagcaagc cgctggcaac ctttttcaac gtatcgtgaa cgtctgtgag 43320 tttggataac tcaacatcaa cttcttcggt tgtt ttggcg attgctgcct ttaaatgttc 43380 cgcactaaca cgaacatagt cgccttcaat ttggacaacc atccattctt tactggttgg 43440 tctatcgcac tcacgcactg cacgattgcg gtcttcgttg tgacgcttca actctttgat 43500 gcggt ctggc aaatctttat agtccattgg attctccgct atggattatg ctggtgggta 43560 gtctactgca tcgtttgcat aaatgattaa ctcatccagc ttcactttgc gtgcatcaat 43620 ttcatccatg cggtgtttta aacctgcaag aagaaactga gtgtaaactt tgaactgttt 43680 gacacggcct gggttcatca gaattatttc tgcaactgag tcatccgtaa caacaccacc 43740 gggaaccggt tctttgaacg attcaacaat caaatcaaag ttggttt ggt gttcggcacg 43800 ctcttcttcc aaccttttat actcttcggt cagaaggaaa acttctgtat ccgtttgagt 43860 actcatgctg gcaactcgaa atgaggcatg tcaacaaagc ttgatggtgg cttttgattt 43920 tcatcaatcc gcgaccaatt accgccccaa c gaatggtga tgccaagctc cttcgccgct 43980 gcaaacatgt gatgcgcgag gatgcggaaa cgttcggtat cattccagtc gattggatat 44040 ggagccaagt caacagcatt gccggacata tgccgagacg tcataacttt ggttgcaccc 44100 tgtgcaaaca attcagcctg acgtttctga ctgcgtaaac cttcaatcac agtcacatca 44160 aaaggcaagg tttcaaatgc cttcgcacaa acctgacgca aatccatacg g acttggttc 44220 agctttgcct gacttgatgg actcagtttg acgttactca tgccgaaact tctccaaccg 44280 gtgttgcgtt tgcagctggt ggatttgtta atccatcaat tgcctggtcc agcgcttgct 44340 gcaaatttgt ttgaataccg gatag attgc cttggtattg catcaatttg accagtttgt 44400 cattttgcaa cggccagaaa gatgcgaggt caacttctgt ccgtgactga gtttcttcaa 44460 caaagacact aagagttttt gtgcctgagt catacatctc agtcagttta tcgacaaaag 44520 atttctggtc agcagcggca tcagcggcag cctgaacagc tgcgcctaat tcgtttaact 44580 gcatggtaat ccttttggat ttacggttag tttgaatttc actaactcgt tattgatttt 4464 0 cgtatagctt gaaacaacag cttcatggcc atacatttcc gcagcggcat gtttaccgat 44700 gcgctccaaa tgacggatta ccttatcggt aagtcgtggg aacactgctt gagagcactc 44760 ggactcgata agatagtcgg ttccgtcaat tttgaactgg ctgtgaatac ct ttcaactt 44820 tagcgtttct ccactgattg aaaactttgc ctgcacgctg gtggagaatt gccaacagct 44880 catcggatgt taaatccaaa tcaactgcta atccttctcc agcatcggca acaactttgc 44940 ctttcaccaa atgtccattc ttgactttat caatgatggc agttagacgg cgatattggt 45000 attcgaggtc tattttgtcc ggcacggcaa tgttctctct ggtttaaatg cgtttgcata 45060 atctgtgtct ttataaaatt cgacatagta tgcacctgaa taattcttcc cgactttggc 45120 aactttacca gagtcgagat tgtaaaagat ttggactggc ttgtccgaag gaacctgcat 45180 ccagattcct tttgtccgaa caacctccaa caagatggga ttcttgtctt gttgggta aa 45240 gatgagacgc tcaccattgc actcaaaggt gacttgatgc attgatgcat ggactggatg 45300 cgaacaacca taaacaacta aaatcgcgaa actaatccag aacattgctg ataattttag 45360 taccatcttc atcgtaatca atcctgtaat taatgtttag cagatagtta cgaacttctg 45420 cccaatgggt tgttgtcatc tttccataga aaggatgaga tttgtgagtt ttattcacct 45480 tcacacgtct ggctaagaca cgaatgtata ctttctttgt ccacttactg tacgaaactt 45540 tatgctggtg gaacttaatt cgcaatgctt catactgctc aggagattga ttctcgtgaa 45600 cagtaatttg atgcgtgtac atggaccgat tgtaatggtt aggccaatca ctacaataaa 45660 tgaaattttg gtagtag acg tacataggac ctccaattga acctattcag gctcctatcg 45720 gagataagaa cctgtaagat ttaactgcaa ctcacagaga attgatgtgt tcaccaattt 45780 ctttgctgag tgttgccatc ttaaccttga tagagttaag tttggctggg tctgcttcac 45840 cgtgatattc ttggtcgaat tcaacaatca atgaagaaat ctttgattgc tttgtctgaa 45900 tttgaaccag agtatcgtat gttgcgtcaa aggtttgaac ctcttcctta tccatttgtt 45960 accttattgc tttgttgaat aaattaactt aattgaggtc actcctatcg gaacaacctc 46020 tcaaaattaa cttggtttct taatcattc aatttcgttt ttcaaacgat tgaaacaagc 46080 acgcattttc tcgtgcacca tttgctgaac aatcaccgaa tcaacttcga aattggtgtt 46140 tgccaacatc actttttcag gtaattcgtt cgcattgtca tactgctcaa gcagcacgtt 46200 caactcagac ataatctcaa ttgaattgct aatatgcaga tgttttgtgt gcacgtaagt 46260 tcttctctga atgttcgtga acaaacctaa tttaacagac agtggaatat gttttggatt 46320 ctctgccgga actaaattaa gtttcgcata tggtga attg cgatgcaggt agtttgtttc 46380 tacctgcatc aaatcaccct cgtaataaat aatgtcgcca gcacgtaatt cactggcgta 46440 cacagtttcg aggaattggc tcattagtta cctatgattt ctcgcagcat cctgcgctct 46500 tgtttaagaa ctcgctgca a atcaaaaggg aatgtcgtca tcaaaatcca ttggcggttc 46560 gttgttctga gtcgctggtg gagtttgctg agctggagct tgctgctgtg gctgagattg 46620 agccggagcc tgttgctgtg gttgctgctg ttgagcaccc tgctgaggac gttctttctc 46680 tgcacgtttc gccatcattt tgtcaacttc atcctgagtc atcagagtga atgccaaaga 46740 tgtgaaagtg cggttgttgg atttctttc ccaacct a gttacagcg tcattgctgt tcttcacatc caactaatcg 46980 tgaatattaa aacgcactca atgaatgcgc tttgtataca gattaatttt cttttgtact 47040 tgacgaatga gtggagcata gaaagagtaa tcatttacat taaatctaga tttctccaga 47100 tatctgtcac gaccatgcag tccgtgtatc aaagattgtt tcacactttg atattcagac 47160 aacatagaca tgagatgcct ctttctatct tgcttccatc tgtttatata tgttctcata 472 20 tcagaaaatt gtcggaatat gtgaaccaaa gaagagccag aagattacag caattgtaat 47280 ccaccagccc aagccaccgt tacgggattt gcgagcttta cggaaagtaa atgattcgcc 47340 accataacca tttttgaatg aagaaccata ccacataaag ttgctcctgg cctatc ggcc 47400 cactggaatt ggatgataca aatgagtcac cggaacacgg cgcttattcg cagttggact 47460 caaatgcaca acattgaaac gatggtcaca cttaattaag cggatatcgc caaagtcgtc 47520 tttaacacac atttggtaat cgttaatgat aaacgcacca attcgaccat cactgcgact 47580 gatatcaccg tcaccgtaac cagcaagaac ttcataaaca cgccctttag tgaacgcgtt 47640 tt ctttgatg ctggctttgt tgatgaaagc tttgccgatt gattcacagc attcaactaa 47700 atcacctttg ttcatttgcg cttcgccttc gacatgattt catacaggcc aacaaatgat 47760 gccagaacga aaatgatgat gagtacaaca atcaaaattg gccatgccag cgctcgtgca 47820 acacgattac tgaaatgacg atgtttcttg ctcaaccagc attgcaagaa taaatcaatt 47880 gcagcaataa tcagatacgt ttgtaccgtc ataatgccga ggatagaata attcagaaat 47940 tcgttcattt cggttctcga cttccataag ttgtgccgca agcattgcag cgatagattt 48000 ttggattctc ttttctggc ttgtggaagc aattgcaacc acaagaacag ttaaagtctt 48060 ttcc agcaac tgtcaggacg aaattcttta aacttccgtc ctcgttctta acagcatttg 48120 cgtacttcat ttgcgaggat tcacaaaatg aactacaccc aattcatcaa cgtaatatga 48180 catattacac cgtccattcg ccaaagtttg ccttgctcgc tgcaacatca tagtttggat 48240 gttttggagt caaagaacag aaggttttct caccacggtc gttgattacg acaaagtgat 48300 tttcagtaac acaagcccac tgataatgag cgccaatata agaacgcttg tcctcgctcg 48360 atagtgacgt tgcatagact ttatatcctt tattcgcggt taatccgaga acagttgaag 48420 attttgaaat cgttgcaatg tcgccaacac gaacgcgggc tttggtcatt tgaccgagtt 48480 tgttt ctgcg tagaatcgcc atttactgct cctatgaagt taatgatagt ttggctgcat 48540 cttgccgaca tttaaataac atttggtatt tgttcagact gcttatagtc accggcaact 48600 taatcctgct atgaagtagt gagaacgaat acaaaagcta cttacgttta tgagctggag 48660 ta tccagcct ttttgcgctc gaaccgcatt attgagccat cgggagtttc attgttgtca 48720 actcaaactt gatatgtaaa ccttcgcgaa tggtgaaaca gggcataagc ccaaagccgg 48780 gattcgcttg gtgtaactgg agtcaggact ttaacggttt gatgcctgac gagttgcgat 48840 ttgttctggc tcagaaacaa tatcgccata acccacatcc agtttatgaa aattatcttg 48900 atacagccac caatgcacga agttctgtta attc acgctg cattgctgct tgttgctctt 48960 ccatcatgcg aatgtgttta tccgcaaaca gaataattgc tgcattagtt gtgattggaa 49020 cattctcaat caaacttact gcattgtcgg ctttagtttg agcacgacga actacgttat 49080 taaacgcaat cagacttgac at ttttatac ctctacaggt aagccagagt tgtgaaaggc 49140 tgcgctttca tcatccacag tattcatcga attcttccca tgctttgtac aacattgaat 49200 cagttgagat attcatgatg ccattattta cgaattcggc accatttatt tcaggatatg 49260 cgtcggacag aaatgcgagg aaatcttgga gcggttgacc ttgtaattga atcactggaa 49320 taatcctccc aattctgcat taaacggacg aaaatgagct gtagaacaa a aacgtgtcct 49380 acctttatca tcaattagat tgaaagttct ctcactgtgg tgaatcattg tgcccaactt 49440 taaagcaatt ggactcacat taacttcacc ttcacctgag acaacttcat aacttttgta 49500 agcagtgaac ccgtcgagac gagcttcaac atgcttcat c acaacacgtt caccagtttt 49560 cattatagac ctgtgtttga caagttgatg ccaatgagct tggcttgata gttgcacaca 49620 tcttcacagt aaagttgatt accagcacga taccaccggt tatcatcata atctctcata 49680 tagaaatccc aaccgtgacg ttgctcaatc caatgccgga gaatcaaacg tcctccggga 49740 gttcgataca atttgttctt acaatcgaaa cgactcattc aatcgtctcc ca aaattcac 49800 agtatgtaat cagtgattca atatcatcac cagttgtacc atcaacatct tccaacagta 49860 catattcaac aacagtttca tccaaacctt tcagatgaac tttcaattca gacaacgtac 49920 cttcgatttg aacttcgttg gcacggtcac gatatttga t aaacagaatt gccattattc 49980 gtgctccaat tctacttcac aatcgtcaag aatcatgatt gctttgctgc tatctttgca 50040 ttcgacagca ttatagaatt cagattcttc caaaactttc cagaacacag tttcaccaac 50100 ggcaaactgt tgaccatctt tcaaatcacc gaaacgagcc ataattttgt atctccaaag 50160 taaattaaga agggcaccat gtgactagca tagtgcccga tgtacatcct aatgatagtt 50 220 tggagcctat ctcgcaactt actcagccaa aatcaccgaa taaattgaga gataaactct 50280 ttgtctttca ttcacatgca actaatgtgg aacaatactg tgaggccaaa tctcacttaa 50340 ttgtcacatt acacatgcgg gtcttgtaac tatcccagac tcgaaact ca aatgtatttg 50400 gcacattcaa agttcagaaa tctggtacag ttgagtgacc agccaacaaa agaaagaaaa 50460 agaataaatc ttttaattcc caatattgtc acaaatatag tacctgtgat tatgctaaca 50520 ggtttagcca aacccagtaa ccatgcgggt tacagtatta catgcagaaa actgtggcaa 50580 tattacatgc atctatttgt cacaatattg caaacgagtc acatgccaat ttgaccagtc 50640 aatcgtatct tcga ggtgat tagtcctcta acaatcggtc acatattaac atgcagaagt 50700 aatcgcaata ttgaaacgat attaacatgc catactgcca caaattttgt tactaatttt 50760 atgtcggctt cgggcttagt taacgcaacg aaacgccact gaacccgaat gccactcact 50820 tctc gatttt gtcaccacta ttccatttga gccagcaagt tccaattcaa tcccggcttg 50880 tgttagggcc tttataatag gattacggaa ctcagtaact cacatggaac atttggacaa 50940 tctcttctcg gaacaacaag ttcttactca gccatggatg ctggaacttt gagtagccat 51000 ctcagggagt tctacagttg actaaacctg taacatcact gtgactaagc tgacatgctc 51060 aatactgttg tacaccag a ataagcgtg ttcgctcatc aggagttctt ctgttacgat attggtatgg aacatatcaa 51300 tgccaccaat ttgtttgctc aatgcttcga attgattatt cttcacaaag atttcaacat 51360 atgcctgacg aattacatcc atcatcttag gagcatctgc caagcagcaa cggaagcaat 51420 cgtgaattga agtgaaacca cgaagtccag ctttctttgc atacttggca acagtacgtg 51480 caaccaatgc atcaatacct tgaatgtagt taacaacaaa ggtacgaaca aattcttcta 51540 cagttggctg tgtctcacga atgctccaag ctttgccttc attctgttgt ccaaagatga 51600 cacgagttgt agagtcaaca cgaatcgaga atgggtcaca gatttcacgt gatgggtagc 51660 aaggtttgtg aaccacttgt ccatcagtgt ggcgataatc aatgtagagt ttagattctt 51720 tctcacattt ggctttaaca gcttccatca ctttagcgat gaacatgtta atcttttcac 51780 caagtgcagc tttcacagct tcaacagcaa gttcagcaaa tgcttcaact ttatctgcac 51840 caaagatttc agtaacagtt gctacgaagt ctttgttacc tgtcagagct ttcttgccac 51900 caccatattg aacagccatg tatggagtct tgatgaagtt acgtccatct ttgcgattga 51960 aatatttctc ttttacttca tcggtaggtt tcattgaaga cttgtctaac agtttcaaca 52020 attccaccag cgacaattga tatgggtcag ctgctttaga atctgagcca actaatccag 52080 ttgcc tgtgc catttgcatg ttgcctgcaa tgaatgccag atactgagta ccagaacatt 52140 ttgcatctaa gccaaagcca acacgtgaat cacactcacc agttgtttca aacttgaacc 52200 aatccaatgc cagacgaatg taagtgaatg gcttcttagg agcgtcacca gaacgtaaca 52260 tgtgagcaag agctccaact gggttctgag caacacgagt taacatgcga gcttctgtcc 52320 acttgccacc agaaatgtca ttcaattcag ccatgaacat ctggta tgct gcactgtctt 52380 tcttcacaat gttctcgatg ttgtgagaat agagtgaacg agcaaagtca gaagactgtg 52440 gatttggacc agcacatgca acgtgataca aacgaccacg gttatctgca aagtaatcag 52500 agaacagaac atcttctttc gccatgttct tagc gttgtt ccaaacatgc aactcttgtt 52560 tgataacatc aggaatgtca ccatgagaca tgccgagtac gtcaccaaca atgtcaacca 52620 tggctgcatc aacatggtat tcagttgatt gcaggaagtc gatacattct gtcatcagtt 52680 tggaaggctt acgtttgcca cctttgatga atggcttgcg acgattgagt tctgacacag 52740 cttctgtcag tggagtgcaa gtctctttag ttgaaacgag t tcaatccaa cgctcagtca 52800 tggtgaatga cttatcttct gcaattgata catagccaag atgcttagcc agtccaatca 52860 agttgataga tgggtcaaca tagtggaact gtttcaggtc attgttggta gagttggctt 52920 tcttgattga cgcttctgca aggctaacca tcgcaacggc tggtacaaca tcgtcacgtg 52980 tgaatgacgt catcatgtgg aagatagcga gagcggcaat agcacaagtc tgtggctgca 53040 tgtagctctg tgtttcttta gcacggaaga tagcagaagt gatagaagga acaacagatt 53100 taacaacgtt tgaattagtc atgatatagc tccaaggttt tatacatagt caactaagcc 53160 cacaaaccag tgccgttgtc ggatgaaacg gatttagttt gcgtagccat tacactattg 53220 atacc taagg gagctgatgt agatgcgttg ttatgcctgc gaagcaggag ctactttggt 53280gttgatttga gtccgatagg acgaattgga aaaagaagt 53319

Claims (19)

A bacteriophage having a lytic activity specific to Erwinia amylovora, Accession No. KACC 97040P.
The bacteriophage according to claim 1, wherein the bacteriophage has the nucleotide sequence of SEQ ID NO: 1.
The bacteriophage according to claim 1, wherein the bacteriophage belongs to the Myoviridae family.
The bacteriophage according to claim 1, wherein the bacteriophage is active at 20 °C to 60 °C.
The bacteriophage according to claim 4, wherein the bacteriophage is active at 25 °C to 50 °C.
The bacteriophage according to claim 1, wherein the bacteriophage is active at pH 3 to 12.
The bacteriophage according to claim 6, wherein the bacteriophage is active at pH 4 to 11.
A pesticide composition comprising the bacteriophage of claim 1 as an active ingredient.
The agrochemical composition according to claim 8, wherein the bacteriophage is used for preventing or treating fire blight.
According to claim 8, wherein the agrochemical composition is an agrochemical composition further comprising an excipient.
The agrochemical composition according to claim 10, wherein the excipient is a surfactant or an extender.
According to claim 8, wherein the agrochemical composition is a hydration agent, granular hydration agent, liquefied hydration agent, liquid, water-soluble, water-soluble granules and encapsulating agents in one or more forms selected from the group consisting of agrochemical composition.
An antibiotic composition comprising the bacteriophage of claim 1 as an active ingredient.
A disinfectant comprising the bacteriophage of claim 1 as an active ingredient.
A detergent containing the bacteriophage of claim 1 as an active ingredient.
A method for controlling fruit burn blight using the bacteriophage of claim 1.
18. The method of claim 17, wherein the fruit tree burn is caused by Erwinia amylovora.
Use of the bacteriophage according to claim 1 for the prevention or treatment of fruit tree fire blight.
Use of the bacteriophage according to claim 1 for use in the manufacture of an agrochemical composition for preventing or treating fruit tree fire blight.

Images