KR101790019B1 - Bacteriophage PM-1 and vegetable soft rot controlling composition containing the same - Google Patents

Abstract

The present invention relates to a composition for controlling vegetative rotiness comprising freshly isolated and identified bacteriophage PM-1 as an active ingredient. More specifically, the present invention relates to a composition for inhibiting the proliferation or killing of a Pectobacterium carotovorum species comprising bacteriophage PM-1 (KACC97021P) as an active ingredient. The bacteriophage PM-1 of the present invention has a controlling effect against the rotting of fresh vegetables and can be used as a microbial pesticide for control of rotting of vegetables and can be used in place of an antibiotic. Therefore, it is possible to reduce the possibility of occurrence of antibiotic resistant bacteria, have.

Description

Bacteriophage PM-1 and a composition for preventing vegetative rotiness containing same

The present invention relates to a novel bacteriophage isolated and identified having an inhibitory effect against vegetative soil erosion, and a composition for preventing vegetative soil erosion using the same.

Grape rot disease is a disease that rotates due to unusual smell and stains, often called soft rot. When it gets cloudy, the pectin in the middle layer of the cell wall melts, and at first it looks like water, but it gradually recovers and rotates and becomes like liquid. Grape rot disease is a disease that appears in many vegetables, but also a lot of tomatoes, sweet potatoes, potatoes.

Pectobacterium carotovorum subsp. Carotovorum , which causes rotting in vegetables containing Chinese cabbage, grows at a temperature of 26 to 28 ° C, but unlike other plant pathogenic bacteria, it grows well at about 36 ° C, It is the biggest obstacle to the production of cabbage in summer. Dried bacillus can invade almost all plants including cabbage, and once it breaks in, it moves to other vegetables in the vicinity and spreads the disease.

Recently, with the spread of well-being culture, the consumption of fresh vegetables is rapidly increasing. Especially, the fresh vegetables displayed on the shelves continuously supply water to maintain freshness. Melting disease occurs once in Chinese cabbage or fresh vegetables is very difficult to control, because consumers do not cook cabbage or vegetables, most of it is ingested. In addition, Pectobacterium carotovorum subsp. Carotovorum , a pathogenic bacterium, is known to survive for a relatively long period of time in soil, and its pesticidal effect is generally low.

Therefore, it is urgent to develop eco-friendly and effective control technology. Until now, disease control of various crops has been largely dependent on the development of resistant cultivars and the chemical agents by pesticide use. There are many technical, economical and time constraints in the development of resistant varieties, and even if new resistant varieties are selected, bacteria with rapid propagation speed will develop their ability to adapt immediately, resulting in loss of resistance. Antibiotics, also known as chemical agents, also work in animal germs, creating problems that can promote antibiotic resistance.

As a countermeasure against this problem, biological control techniques are now being emphasized. Antimicrobial and competitive microorganisms can be isolated and cultured against pathogens, treated directly with soil, seeds or plants to prevent pathogens from entering, Is known, and a method using a virus called bacteriophage which specifically attacks and lyses only bacteria is known. Such biological control is considered to be highly desirable in that there is no problem of environmental pollution, weakness and residual toxicity of organic synthetic pesticides.

From 1910 to 1930, when phage was discovered, phage was characterized by host specificity, endotoxins of host bacteria, exotoxins, and other problems that were not scientifically solvable at the time, such as penicillin found by Fingaming in 1929 and Streptococcus The discovery and use of chemotherapeutic agents (drugs), represented by the mycine, have slowed its utilization and development.

On the other hand, there were several problems in using phage as a therapeutic agent at the time. Among them, high specificity, high specificity only for some strains, and high resistance, the resistance of bacteria immediately after use, and the effect as a therapeutic agent was immediately eliminated. On the other hand, the chemotherapeutic agent has a wide range of antimicrobial activity, and since it was not used for the first time, it was widely used. As a result, the pharmacotherapy almost disappeared. Since then, phage therapy has been declared invalid by the WHO in the 1950s and has only been sustained in the Eastern bloc and the Soviet Union.

However, in the 1980s, the idea of phage therapy was changed. In 1982, the British researchers group, Smith and Huggins, experimented with the mouse model of meningitis. The mice were injected into the subcutaneous muscles of the mice and the subcutaneously injected bacterium was subjected to specific microbial phage injection. As a result, 100% of the control mice that did not receive the phage survived 100% It was confirmed that the effect of antibiotics (such as streptomycin) was superior. At this time, the resistance problem of the phage could be solved by using various types of phage as a cocktail.

The size of the phage is about 1 / 10th of the size of the bacterium, and the shape of the phage must be observed with an electron microscope. However, if the phage lyses the bacteria, transparent spots (plaques) are formed in the bacterial colonies grown on the agar medium, so that the presence of the phage can be visually confirmed.

Phage develops about 100 ~ 200 new phage in about 30 minutes after bacterial infection and comes out by destroying the membrane of bacteria. Bacteria undergo two proliferation, the fastest being two individuals in about 20 minutes, four individuals in 40 minutes, and eight individuals in 60 minutes. In the case of phage, more than 100 new phages are produced in about 30 minutes, which is far superior to bacteria in the rate of proliferation. If a specific bacterium proliferates unlimitedly once every 20 minutes, it takes only 36 hours for the germ from one cell to weigh the same as the earth, so phage can be considered as an important candidate for inhibiting bacterial proliferation have.

A phage is classified into a lytic and a phage phage. A virulent phage and a cycling cycle are defined as a temperate phage. Temperate phage inserts phage gene information into the genome of the host bacteria, so that the shape of the phage disappears and host bacteria are not destroyed. This condition continues as long as the host bacteria are in good environmental conditions. If the environment of the host bacteria is deteriorated, the phage gene in the gene of the host bacteria is converted into a lytic cycle after the phage particle is produced and the host bacteria are lysed, Escape out.

On the other hand, several advantages of phage compared to antibiotics are listed below. First, phage can only be replicated if there is a host. Second, the phage exists in a natural state and exists wherever bacteria exist. Third, phage is safe for any eukaryotic organism. Fourth, it is safe for other bacteria that are beneficial because of its high specificity. Fifth, phage is very easy to prepare.

Due to the above-mentioned various advantages, more and more grips are being developed in recent years.

Accordingly, it is an object of the present invention to provide a novel bacteriophage capable of inducing the death of aerial bacterium, and to provide a composition for inhibiting proliferation of the aerial mycorrhizae or containing the same.

In order to achieve the above object, the present invention provides newly isolated and identified bacteriophage PM-1 (KACC97021P) exhibiting a controlling ability against vegetable rot disease.

The present inventors collected a soil sample of a cabbage field in Pyeongchang-gun, Gangwon-do and isolated and identified the bacteriophage of the rotifers present in the soil. The bacteria bacteriophage of the isolate bacteriophage and the host specificity were evaluated, , And named PM-1.

As shown in FIG. 1, the micrograph of the phage PM-1 revealed that it had a 55 nm head and a tail of about 120 nm, and had morphological characteristics of Myoviridae. , Which was deposited with KACC (Institute of Agricultural Biotechnology) on November 12, 2013 and granted the deposit number KACC97021P.

The homology of the nucleotide sequence with the BlastP program showed that the phage PM-1 showed high homology with the enterobacteria phage FEcoM-GJ1 and Erwinia phage νB EamM-Y2 and was very similar to other known phage . The previously reported Pectobacterium carotovorum subsp. There was no similarity of sequence with carotovorum phage PPP1 (KACC97005P), MY-1 (KACC97009P) and T10-1 (KACC97008P).

The bacteriophage of the present invention can be confirmed to form plaques by lysing rot fungi in experiments conducted with host plants of rot fungi. Particularly, the bacteriophage of the present invention is preferably a Pectobacterium carotovorum species, more preferably a Pectobacterium carotovorum subsp. Carotovorum or a Pectobacterium carotovorum subsp. scanning the fact that (Pectobacterium carotovorum subsp. brasiliense) to be specifically lysed was confirmed by experiments.

Accordingly, the present invention provides a composition for preventing rot fungus disease comprising bacteriophage PM-1 (KACC97021P) as an active ingredient.

The present invention also relates to the use of a Pectobacterium carotovorum species, more preferably a Pectobacterium carotovorum subsp . Carotovorum or a Pectobacterium carotovorum species, subspecies Brazilian switch (Pectobacterium carotovorum subsp. brasiliense) can provide the composition excellent proliferation inhibitory effects and apoptosis, such a composition comprises a gripping PM-1 (KACC97021P) as an active ingredient.

The composition for controlling rottenness according to the present invention may be used for, but not limited to, vegetables and fruits such as lettuce, radish, Chinese cabbage, tomato, sweet potato, potato, etc., in which blight may occur. In particular, the present invention relates to the use of a Pectobacterium carotovorum species, more preferably a Pectobacterium carotovorum subsp . Carotovorum or a Pectobacterium carotovorum subsp . Pectobacterium carotovorum subsp. brasiliense) as is effective for the control of soft rot of Chinese cabbage or lettuce is because of the onset.

Meanwhile, any one selected from the composition for preventing vegetative rotiness of the present invention and the composition for inhibiting or preventing the growth of Pectobacterium carotovorum species may be used as a biochemical pesticide for the purpose of stable formulation suitable for actual packaging A wetting agent, a liquid wettable agent, a liquid agent, a water-soluble agent, a water-soluble granule, or an encapsulating agent using a surfactant or an extender. Examples of the surfactant include alkyl (C ₈ -C ₁₂ ) aryl sulfonate, dialkyl (C ₃ -C ₆ ) aryl sulfonate, dialkyl (C ₈ -C ₁₂ ) sulfosuccinate, lignin sulfonate, naphthalene Sulfonate condensates, naphthalene sulfonate formalin condensates, alkyl (C ₈ -C ₁₂ ) naphthalene sulfonate formalin condensates, sodium salts of sulfonate compounds such as polyoxyethylene alkyl (C ₈ -C ₁₂ ) phenylsulfonate, or calcium salts, alkyl (C ₈ ~ C ₁₂₎ sulfates, alkyl (C ₈ ~ C ₁₂₎ aryl sulfates, polyoxyethylene alkyl (C ₈ ~ C ₁₂₎ sulfates, polyoxyethylene alkyl (C ₈ ~ C ₁₂₎ phenyl sulphate , Sodium salts or calcium salts of succinate compounds such as polyoxyalkylene succinates, sodium benzoates, anionic surfactants such as alkylcarboxylates, polyoxyethylene alkyl (C ₁₂ to _C18 ) ether, poly Nonionic surfactants such as polyoxyethylene alkyl (C ₈ -C ₁₂ ) phenyl ether, polyoxyethylene alkyl (C ₈ -C ₁₂ ) phenyl polymer, ethylene oxide propylene oxide copolymer, polycarboxylates such as Triton 100 and Tween 80 It is to be understood that those skilled in the art will readily understand that surfactants other than these surfactants can also be used.

Examples of the extender include bentonite, talc, clay, kaolin, calcium carbonate, silica, pumice, diatomaceous earth, acidic clay, zeolite, pearlite, white carbon, ammonium sulfate, urea, glucose, dextrin, , Yellow loess, glucose and starch, water, etc. may be used singly or in combination of two or more kinds. It is to be understood that other extender may be used as well, and it will be easily understood by those skilled in the art will be.

As described above, the bacteriophage PM-1 newly isolated in the present invention has a controlling effect against the rotting of fresh vegetables, and can prevent the loss due to corruption during the distribution and storage of agricultural products after harvesting, and the microbial pesticide As shown in FIG.

In addition, since the bacteriophage having bacterial growth inhibition or killing ability of the present invention can be used instead of antibiotics, it is possible to reduce the possibility of occurrence of antibiotic resistant bacteria and improve environmental safety by reducing the use of antibiotics.

Figure 1 is a plaque photograph formed by bacteriophage PM-1 isolated from a soil sample.
2 is an electron micrograph showing the non-shrinkage type of bacteriophage PM-1 isolated and identified in the present invention.
3 is an electron micrograph showing the contraction type of bacteriophage PM-1 isolated and identified in the present invention.
Fig. 4 is a graph showing the activity of the host lysate according to the concentration of bacteriophage PM-1.

Hereinafter, embodiments of the present invention will be described in detail to facilitate understanding of the present invention. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the invention are provided to more fully describe the present invention to those skilled in the art.

Example 1: Bacteriophage separation

In order to isolate bacteriophage from cabbage rot caused by plants grown on cabbage blight.

Pectobacterium carotovorum subsp. Carotovorum Pcc1, which is the host, is cultured for 16 hours in a 50-mL tube, and 20 ml of soil and 10 ml of 10 mM MgSO ₄ are added to each soil sample. . After incubation for 16 h at 28 ° C at 180 rpm, the suspension was centrifuged at 4000 rpm for 5 min and filtered (0.45 μm) to remove the residue. 0.1 mL of the above sample and 0.1 mL of the broth of cultured Chinese cabbage cultured for 12 hours or more were pipetted and mixed. This mixed solution was mixed with agar (0.4%) medium, poured into a plate containing 1.5% agar medium, and cultured at 28 ° C for 6 hours.

When the desired bacteriophage appeared on the plate, an independent and transparent plaque was observed (Figure 1). The plaque area was separated by using a platinum loop, and then it was placed in a new bacterial culture solution, and the culture solution was clarified.

Thereafter, the culture solution was centrifuged, and only the supernatant was collected, and the filtrate containing only the bacteriophage was recovered. The recovered bacteriophage filtrate was purified by ultrafast centrifugation using a cesium chloride (CsCl) density difference to obtain pure bacteriophage.

The bacteriophage thus obtained is a Pectobacterium carotovorum species including Pcc1 , in particular Pectobacterium carotovorum subsp . Carotovorum or Pectobacterium carotovorum subsp . Subsp. (Pectobacterium carotovorum subsp. brasiliense) are excellent killing effect specifically on, and it was named as PM-1, deposited with the agricultural biotechnology Research Institute at 11 December 2013 the date was given an accession number KACC97021P.

Example 2: Shape and classification of acquired phage

The electron micrographs of the bacteriophages obtained in Example 1 were analyzed (Figs. 2 and 3). The electron microscope used an Energy-Filtering Transmission Electron Microscope. The bacteriophage sample to be observed was placed on a carbon-coated copper grid and negatively stained with 2% liquid uranyl acetate. The dyed samples were observed using an energy filtration transmission electron microscope (LIBRA 120, Carl Zeiss) at a voltage of 80 kV.

The bacteriophage PM-1 thus observed was found to be a bacteriophage of Myoviridae consisting of a head of about 55nm and a tail of about 120nm as a standard for sorting.

Example 3: Bacterial bacterium analysis of bacteriophage PM-1

The host pcc1 culture medium was mixed with a constant concentration of bacteriophage PM-1, and the absorbance was measured at regular intervals and compared with that of the control group. FIG. 4 shows a graph showing the activity of the host bacteria according to the concentration of bacteriophage PM-1. When MOI (multiplicity of infection) was 0.01 or 0.1, the absorbance gradually decreased after about 5 hours, and when the MOI was 1, the absorbance decreased sharply after about 3 hours. Bacteriophage PM- . Also, it was confirmed that no resistance to the phage was observed even after culturing for 20 hours or more.

Example 4: Sequence analysis of obtained phage

The phage gene was isolated for the sequencing of isolated phage. 0.5 M EDTA (pH 8.0), proteinase K and SDS were added to 1 mL of purely isolated bacteriophage, and the concentrations after addition of reagents were 20 mM, 50 ug / mL, and 0.5%, respectively. The mixed solution was kept at 56 DEG C for 2 hours and then cooled at room temperature. The same amount of phenol was added and the mixture was centrifuged at 3000 g for 5 minutes. Only the supernatant was obtained, and PC (phenol-chloroform) was once again treated and centrifuged under the same conditions. The same amount of chloroform was added to the supernatant obtained above and centrifuged. This procedure was repeated, and 1 mL of 95% ethanol and 50 μL of 3 M sodium acetate (pH 5.2) were added, followed by centrifugation for 15 minutes, followed by further centrifugation with addition of 70% ethanol. The ethanol was removed, the DNA was dried, and the DNA was dissolved in 1X TE buffer.

The nucleotide sequence of the purely isolated total gene was determined. The nucleotide sequence of bacteriophage PM-1 was 55,098 bp (SEQ ID NO: 1) in total, and the nucleotide sequence was shown in SEQ ID NO: 1. The determined nucleotide sequence was analyzed using the 'Blast program'. The genome of PM-1 contained 2,665 bp terminal repeats out of 55,098 bp. A single tRNA encoding cysteine and 63 open reading frames (ORFs) Was predicted. Of these, only 21 ORFs are homologous to known proteins and 42 ORFs are predicted to be hypothetical proteins. The 21 ORFs can be divided into four groups: metabolism, packaging, structure (sturcutre), and lysis. The tail fiber protein, which determines host specificity, has very low homology with other known proteins and can support the strong host specificity of PM-1 and can be used as an integrase to maintain the bacteriophage in a pooled state. And repressor were not predicted, thus confirming that PM-1 is phagocytosis. The estimated functions of the PM-1 gene are shown in Table 1 below.

Locus tag Length (aa) Description BLASTP best match PM-1_001 642 DNA-dependent RNA polymerase RNA polymerase [Enterobacteria phage phiEcoM-GJ1] PM-1_002 117 심포치 gp09 [ Erwinia phagevB_EamM-Y2] PM-1_003 73 심포치 gp11 [ Erwinia phagevB_EamM-Y2] PM-1_004 83 심포치 hypothetical protein phiEcoMGJ1_gp09 [Enterobacteria phage phiEcoM-GJ1] PM-1_005 93 심포치 hypothetical protein phiEcoMGJ1_gp12 [Enterobacteria phage phiEcoM-GJ1] PM-1_006 96 심포치 hypothetical protein phiEcoMGJ1_gp14 [Enterobacteria phage phiEcoM-GJ1] PM-1_007 104 putative NrdA.1-like protein NrdA.1-like protein [ Cronobacter phage CR9] PM-1_008 125 putative single-stranded DNA-binding protein ssDNA binding protein [ Erwinia phage vB_EamM-Y2] PM-1_009 143 심포치 hypothetical protein phiEcoMGJ1_gp17 [Enterobacteria phage phiEcoM-GJ1] PM-1_010 130 심포치 gp23 [ Erwinia phagevB_EamM-Y2] PM-1_011 101 심포치 gp25 [ Erwinia phagevB_EamM-Y2] PM-1_012 159 TIGR02594 family protein hypothetical protein Entcl_1861 [ Enterobacter lignolyticus SCF1] PM-1_013 189 가타타제 gp28 [ Erwinia phage vB_EmM-Y2] PM-1_014 86 심포치 hypothetical protein phiEcoMGJ1_gp23 [Enterobacteria phage phiEcoM-GJ1] PM-1_015 117 심포치 syrP protein [ Pseudoalteromonas flavipulchra ] PM-1_016 76 심포치 hypothetical protein phiEcoMGJ1_gp25 [Enterobacteria phage phiEcoM-GJ1] PM-1_017 103 심포치 gp32 [ Erwinia phagevB_EamM-Y2] PM-1_018 224 심포치 gp34 [ Erwinia phagevB_EamM-Y2] PM-1_019 120 심포치 hypothetical protein phiEcoMGJ1_gp29 [Enterobacteria phage phiEcoM-GJ1] PM-1_020 74 심포치 N-acylneuraminate cytidylyltransferase [ Streptomyces collinus Tu365] PM-1_021 91 심포치 hypothetical protein [ Citrobacterfreundii ] PM-1_022 60 심포치 hypothetical protein [ Escherichiacoli ] PM-1_023 90 심포치 peptidase M48 Ste24p [Proteobacteria bacterium CAG: 495] PM-1_024 218 삼이일리이트 synthase thymidylate synthase [ Erwinia phage vB_EamM-Y2] PM-1_025 563 DNA primase / helicase DNA primase / helicase [ Erwinia phage vB_EAMM-Y2] PM-1_026 645 DNA polymerase DNA polymerase [Enterobacteria phage phiEcoM-GJ1] PM-1_027 288 심포치 gp52 [ Erwinia phagevB_EamM-Y2] PM-1_028 357 5'-3 'exonuclease 5'-3 'exonuclease [ Erwinia phagevB_EamM-Y2] PM-1_029 175 HNH endonuclease domain containing protein gp54 [ Erwinia phagevB_EamM-Y2] PM-1_030 256 putative DNA ligase putative DNA ligase [Enterobacteria phage phiEcoM-GJ1] PM-1_031 197 putative ATP-binding protein gp56 [ Erwinia phagevB_EamM-Y2] PM1_032 150 deoxyuridine 5'-triphosphate nucleotidohydrolase deoxyuridine 5'-triphosphate nucleotidohydrolase [ Erwinia phagevB_EamM-Y2] PM1_033 43 심포치 gp58 [ Erwinia phagevB_EamM-Y2] PM1_034 132 심포치 gp59 [ Erwinia phagevB_EamM-Y2] PM1_035 119 심포치 hypothetical protein phiEcoMGJ1_gp45 [Enterobacteria phage phiEcoM-GJ1] PM1_036 61 심포치 hypothetical protein phiEcoMGJ1_gp46 [Enterobacteria phage phiEcoM-GJ1] PM1_037 671 종단 putative large subunit terminase [Enterobacteria phage phiEcoM-GJ1] PM1_038 72 심포치 hypothetical protein phiEcoMGJ1_gp49 [Enterobacteria phage phiEcoM-GJ1] PM1_039 445 portal protein putative portal protein [Enterobacteria phage phiEcoM-GJ1] PM1_040 353 부사 gp65 [ Erwinia phagevB_EamM-Y2] PM1_041 158 심포치 hypothetical protein phiEcoMGJ1_gp52 [Enterobacteria phage phiEcoM-GJ1] PM1_042 338 major capsid protein major capsid protein [Enterobacteria phage phiEcoM-GJ1] PM1_043 146 심포치 hypothetical protein phiEcoMGJ1_gp54 [Enterobacteria phage phiEcoM-GJ1] PM1_044 126 심포치 gp70 [ Erwinia phagevB_EamM-Y2] PM1_045 121 심포치 hypothetical protein phiEcoMGJ1_gp56 [Enterobacteria phage phiEcoM-GJ1] PM1_046 172 심포치 gp72 [ Erwinia phagevB_EamM-Y2] PM1_047 483 심포치 phage protein [Enterobacteria phage phiEcoM-GJ1] PM1_048 152 심포치 phage protein [Enterobacteria phage phiEcoM-GJ1] PM1_049 151 심포치 hypothetical protein phiEcoMGJ1_gp60 [Enterobacteria phage phiEcoM-GJ1] PM1_050 53 심포치 hypothetical protein phiEcoMGJ1_gp61 [Enterobacteria phage phiEcoM-GJ1] PM1_051 1280 가타이 타일 섬유 putative tail fiber [Enterobacteria phage phiEcoM-GJ1] PM1_052 366 심포치 hypothetical protein phiEcoMGJ1_gp63 [Enterobacteria phage phiEcoM-GJ1] PM1_053 300 심포치 phage protein [Enterobacteria phage phiEcoM-GJ1] PM1_054 124 심포치 hypothetical protein phiEcoMGJ1_gp65 [Enterobacteria phage phiEcoM-GJ1] PM1_055 264 부타제 putative baseplate assembly protein [Enterobacteria phage phiEcoM-GJ1] PM1_056 123 심포치 phage protein [Enterobacteria phage phiEcoM-GJ1] PM1_057 389 심포치 phage protein [Enterobacteria phage phiEcoM-GJ1] PM1_058 216 심포치 phage protein [Enterobacteria phage phiEcoM-GJ1] PM1_059 385 심포치 hypothetical protein phiEcoMGJ1_gp70 [Enterobacteria phage phiEcoM-GJ1] PM1_060 454 심포치 phage protein [Enterobacteria phage phiEcoM-GJ1] PM1_061 626 심포치 hypothetical protein [ Brenneria sp. EniD312] PM1_062 118 holin holin [ Erwinia phage vB_EmM-Y2] PM1_063 186 putative endolysin putative endolysin [Enterobacteria phage phiEcoM-GJ1]

Example 5: Host specificity analysis of bacteriophage PM-1

In order to confirm the host specificity of bacteriophage PM-1, the lytic activity of 86 kinds of Pectobacterium carotovorum subsp. And 21 kinds of Pectobacterium sp. Were analyzed.

Host specificity of bacteriophage PM-1 was determined by the dropping method. After culturing for 16 hours, 0.4% agar medium was mixed with the medium. The medium was poured into a plate and dried at 28 ° C for 15 minutes. The diluted phage was dropped thereon, dried at room temperature and incubated at 28 ° C for 6 hours. If the strain is susceptible to phage, a transparent region, i.e., a plaque, is seen on the plate, demonstrating that the bacterial cells are the result of a complete lysis. If the susceptibility is weak, a cloudy area occurs or an independent bold spot appears.

Experimental results, bacteriophage PM-1 of the present invention specification tobak Te Solarium Caro sat borum subspecies Caro sat borum (Pectobacterium carotovorum subsp. Carotovorum) or peck tobak Te Solarium Caro sat borum subspecies Brazilian's high for (Pectobacterium carotovorum subsp. Brasiliense) Luciferase activity. In addition, such specifications tobak Te Solarium (Pectobacterium) by comparing the lytic ability to target the same host to determine the differences between that as the host bacteriophage PM-1 of PPP1 (KACC97005P) and MY-1 (KACC97009P) result, PM -1 showed different host specificities with PPP1 and MY-1. In particular, Pectobacterium carotovorum subsp. Odoriferum , And Pectobacterium atrosepticum showed resistance to both PPP1 and MY-1, but to PM-1. The susceptibility to Pectobacterium species strains is shown in Table 2 below. In Table 2 below, ++++: clear lysis, +++: a little turbid, ++: turbid, +: very turbid, no lysis, and blank spaces are represented by dotting traces.

Host bacteria MY-1 PPP1 PM-1 Pectobacterium carotovorum
subsp. carotovorum KACC 90187 - - - isolate 1 - - - isolate 2 ++++ ++++ ++++ isolate 3 - - - isolate 4 - - - isolate 5 - - - isolate 6 - - ++ isolate 7 - - - isolate 8 - - - isolate 9 - - - isolate 10 - - - isolate 11 - - - isolate 12 - - - isolate 13 - - - isolate 14 - - - isolate 15 - - - isolate 16 - - - isolate 17 - - - isolate 18 ++++ ++++ ++++ isolate 19 ++++ ++++ ++++ isolate 20 ++++ +++ ++++ isolate 21 ++++ +++ +++ isolate 22 ++++ ++++ ++++ isolate 23 ++++ ++++ ++ isolate 24 ++++ ++++ ++++ isolate 25 ++++ ++++ ++++ isolate 26 ++++ ++++ ++++ isolate 27 ++++ ++++ +++ isolate 28 - - - isolate 29 ++++ ++++ ++++ isolate 30 ++++ ++++ ++++ isolate 31 ++++ ++++ ++++ isolate 32 ++++ +++ ++++ isolate 33 - - - isolate 34 - - - isolate 35 - - - isolate 36 - - - isolate 37 - - - isolate 38 ++++ ++++ ++++ isolate 39 - - - isolate 40 - - - isolate 41 ++ ++ ++++ isolate 42 - - - isolate 43 - - - isolate 44 - - - isolate 45 - - - isolate 46 - - - isolate 47 - - - isolate 48 - - - isolate 49 ++++ ++++ ++++ isolate 50 ++++ ++++ ++++ isolate 51 ++++ ++++ ++++ isolate 52 - - - isolate 53 +++ +++ ++++ isolate 54 - - - isolate 55 - - - isolate 56 ++++ ++++ - isolate 57 - - - isolate 58 - - - Pectobacterium carotovorum
subsp. brasiliense isolate 1 ++++ ++++ ++++ isolate 2 ++++ ++++ ++ isolate 3 - - - isolate 4 - - - isolate 5 ++++ +++ ++++ isolate 6 ++++ ++++ ++++ isolate 7 ++++ +++ ++++ isolate 8 ++++ ++++ ++++ isolate 9 ++++ ++++ ++++ isolate 10 ++++ ++++ ++++ isolate 11 ++++ ++++ ++++ isolate 12 - - - isolate 13 ++++ ++ ++++ isolate 14 ++++ ++ +++ isolate 15 - - ++ isolate 16 +++ + isolate 17 ++++ ++++ ++++ isolate 18 ++++ ++ +++ Pectobacterium
carotovorum
subsp. odoriferum KACC10486 - - - isolate 1 - - - isolate 2 - - - isolate 3 - - - isolate 4 - - - isolate 5 - - - isolate 6 - - ++ isolate 7 - - ++ isolate 8 - - + Pectobacterium
atrosepticum KACC 10478 - - + KACC 10480 - - isolate 1 - - - isolate 2 - - - Pectobacterium
betavasculorum isolate 1 - - - Pectobacterium
wasabiae KACC 10061 - - - isolate 1 - - - isolate 2 - - - isolate 3 - - - isolate 4 - - - isolate 5 - - - isolate 6 - - - isolate 7 - - - isolate 8 - - - isolate 9 - - - isolate 10 - - - isolate 11 - - - isolate 12 - - - isolate 13 - - - isolate 14 - - - isolate 15 - - -

In addition, Cronobacter sakazakii , Salmonella typhimurium , Like Peck tobak Te Solarium Bacillus cereus (Bacillus cereus) and bacteriophage PM-1 is their host by checking for having a resistance of PM-1 both by positive bacteria such as Gram from enteric bacteria and strains belonging to the (Enterobacteriaceae), such as (Pectobacterium) Of the present invention. Table 3 below shows strains resistant to bacteriophage PM-1.

G (-) bacteria  / Enterobacteriaceae Cronobacter sakazakii ATCC 29544 Dickeya zeae Enterobacter cloacae Escherichiacoli 0157 Klebsiella pneumoniae Pantoea agglomerans Pantoea ananatis KACC 10059 Rahnella aquatilis Salmonella arizonae ATCC 13314 Salmonella typhimurium ATCC 13311 Serratia plymuthica Serratia rubidaea Shigella boyidii ATCC 8700 Shigella flexneri ATCC 12022 Shigella sonnei ATCC 25931 Yersinia enterocolitica ATCC 55075 G (-) bacteria except Enterobacteriaceae Acinetobacter calcoaceticus Aeromonas hydrophila KCTC 2358 Burkholderia andropogonis Burkholderia gladioli Chryseobacterium balustinum Neisseria meningitidis ATCC 13077 Pseudomonas chlororaphis SH36 Pseudomonas corrugata SH50 Pseudomonas putida SH53 Pseudomonas syringae Stenotrophomonas maltophilia SHG Xanthomonas albilineans Yersinia enterocolitica ATCC 55075 G (+) bacteria Bacillus cereus KCCM 40138 Bacillus megaterium Bacillus thuringiensis ATCC 29730 Lactobacillus animalis ATCC 35046 Listeria innocua ATCC 33090 Listeria ivanovii ATCC 19119 Listeria monocytogenes ATCC 15313 Staphylococcus xylosus KACC 10785

Agricultural Biotechnology Research Institute KACC97021P 20131112

<110> Rural Development Administration <120> Bacteriophage PM-1 and vegetable soft rot controlling composition          containing the same <130> P13-261 <160> 1 <170> Kopatentin 2.0 <210> 1 <211> 55098 <212> DNA <213> Pectobacterium phage PM1 <400> 1 ggagataaat acggtcccta ttccaacttt ttctcaccta tcggttgagt tacaacagcc 60 aaacaagtta ggtgatgagt caacgtcaac accactcgct tcatcattgt gctccacagt 120 tgtatcagtc gtgggatggc tacgctaact aaatccctca ctcatgcatt ctgctaatca 180 tcggcattag ttgaatgtgg atactacaat aattggagct tattatgact atctcaattc 240 aaactactaa atctgttgtt ccttcaatcg ctgcactcgt attccgtgct aagcgtgatg 300 tgtttgctcc acagacttgt gctattgcgt ctctcgcaat cttccacatg ttgacggctc 360 tcactcgtga cgatgttgta ccagccatgt cgcttgttgc acttgcagaa gcgtctatca 420 agaaggctaa ctcatctaac aacgacatga agcaatacca ctatgtcgac ccaaccatca 480 atctgattgg attggctaag cactttggct tcatctcaat gaatgaggac aagtcgttcc 540 aactgactga gtcttggatt gagctcatca caactaagga ttctgcaatc ccatttgtaa 600 cccaatcac ggctgagaat cgtcgcaagc ctatggtgaa aggtggcaag gtcaaagcaa 660 gtaagactct gccgctgca attgatttcc tgcaatcgac cgagtatcac gtcgacctgc 720 aaatggttaa tgtcatcagc aagatgattg acatggattc acgagttggt aagacatttg 780 cacctgcaat tctgcaagaa cttcacgtct ggaatgctgc taagaatatg gctgcacaag 840 atgttctgtt ctcagaatac tttgctgaca atcgtggtcg tttgtatcac gttgcgtgtg 900 ctggcccaaa tccacaatca agtgactttg ctcgttgctt gtattctcac aatgttgaga 960 acgtggtcaa taagctcaat gctgacggct caacaactgc cgcatacaac atgttcatgg 1020 cagagctcga tgacattgct ggtggcaaat ggtgtgatgc aaagatgctc acccgtgtag 1080 cacagaatcc atcaggtgct ttgcaaaact tgttgtctcg tgatgatgca cctaagaagc 1140 cattcacata tgttcgtctg gctcttgatt ggttccagtt cgaaacaaca ggtgcatgtg 1200 attctcgtgt aggatttggt cttgatgcta aatgttcagg tactcaatat ctggcattca 1260 ttgcaggcaa tcttgaaatg gcacaggcaa caggattggt tgattctgat tccaaagctg 1320 ctgacccata tcaactgtct ctggttcaat tgatgaaatt gctttctcgt tcatcttacc 1380 aaccagacca agcaactctc gatgctcacc tgaacaagaa aacaggtcgc aactttatca 1440 agactccata catggctgtg caatatggtg gtggtactgc tgcactgttg ggcaacaaag 1500 atttctgcca gtatgtcaaa cagcagttgc agattgacgt ctctaaactt gatgacttcg 1560 ctgaactttg tgttgatgca gttcatgcag cacttggcga gaagattaac aagttcattg 1620 agcagactca gaaagctgtt ctcgcaatgt gtgtgaaact tggcaaagag ttcttctcat 1680 atcgtcacac tgatggacaa gttgtacaca agccttgctt cccatctgag gaagtatgtg 1740 agccattctc aattcgtgtt gactcacaaa ctcgtgtcat ctttggacaa cagaatgaag 1800 gcaaagcatg gtctattcgt tcagcaaatc caactaaaga ggaatttgtt cgtacctttg 1860 ttgttaacta catccaaggt atcgatgctt tggtggctcg tgttgttgct aaacatgcta 1920 agaaagctgg acttcgtggg tttacatcaa tccatgactg cttccgttgc tgcttggctg 1980 atgcaccaca gatgatggat gtaatcagag ctgcctacat tgaggtgttc gttgataaca 2040 atcaattcga gcatttggct aagcaacttg gtggcatcaa catgtaccat gagaacatcg 2100 taacgaaaga gttgttgatg agcgaacatg cttattactt ctgccagtag ttaaaatacg 2160 ggaaacgaag tttcacgaaa gataagaata attctgttaa aagattattt attattagcc 2220 gtgagacttc gtctcccgcc atctttaaac cgaatatttc agcaatatta ccgcatgtag 2280 tattgctaac atatttggca ttagtaggaa gtgaagtaac agaacacgga gaagtattcc 2340 gcatgtcatg ttgagactgg ttgcaaacag attcagcagg tcaacttgaa gtacaggtga 2400 taatgatagc ctcattgaac tccctgagtt agctgctcaa agttccaatc accatgcatc 2460 aggtacgaat atcatccgaa actgttactg catttaggct acctcatctc cgacaaacag 2520 gagacagcgt agctactcag catgaccagt tgctccaatg ttagttccta ttataaaggc 2580 ccgctcacaa gccgggaaca ttggcgttgg aacaattgga aattgctgag gaattggaag 2640 agaagtgaag aagggaacga gagtcgaaca acaaccctca ctggctgcgg attccatccc 2700 gtaagccgtc ccgaattgag ctataaatat tgtggcaata ttgctgcgac aatggcatgt 2760 aaatttgtga acaaacattg catgtaagaa tgtgaccaat tgttagggga tttaatcacc 2820 cgaagatacg atgacaggtc aatctggcat gtgatttcgt ttataatatt gtgacaatat 2880 tgatgcactt ggtatcagtg ttggcatgtc aattttgctg cgagccttgt cctatctggc 2940 tttgtgctgt ggtaggcagc attatccctg cttaaatatt gtgactgatt tgtggattct 3000 tctttaattt aaattgaagg attctatctt ggtcggtttt ccgttccaac aatcttgtgc 3060 caattacgag atgtttgctt gggatgtgga ggccaatttg aataccaatg tgcatgttga 3120 attcgtgaca gatttcgtca attggcgacg gagtttgttg cagttcagca tgtccatttt 3180 attctaattg catgtgaaat aaggcaaatc tgtattggcc tgcctcaatt tgagctaatc 3240 agcatgtgtt ttcgtgacag aatacatcct gattaagctc caatttaatc attgttgtac 3300 agtttggcac tttgtgtgtc acaagctcct gctttgttgt gggagctatt ctttaatttt 3360 ggaggcttta aatgactttt gaccaattga aaggtggcga actattttca tttgctggtg 3420 tgaacttcat taaagtaatg gaagagtcca gctactataa tgcagtggtg gatggcagtt 3480 catcaatggc ttcttgcttt gctggtagtg aggacgttgg agaaccattg ccaacatttg 3540 caagtttggc tgaaggtgat gtattcacta ttaaggcatt gtctggcgca ttcattaagt 3600 gcgagacttc ttcaagtttg tataatgctg ttgcgcttta tcgtgacagt gcatactaca 3660 acttcatgca atcacaagct gtaactctgg tgaaaaaggt agcggtggag gaagaagagt 3720 gattgtcgaa tggcagtggt cagatgcaga tgctgattcc gaaaaggagt ttgagaatct 3780 gcgagaagtt caggtctggt tgcaaggtca aagagaagaa gatttctctt ggatttactt 3840 gtatgacaac aatgggaatg agcttgcagc aagtttcctg caaattctgg agttgaaatg 3900 acagttctta gcatcggctt ccgtggaatg ccagtcattg acgaagtgtt gtttgcaaat 3960 gccagcagg ccatcaactt cttggaactg aattctgaaa acatcacgca tgtaacattg 4020 ttggattctg cggcagagcc tgtgaaatgt gggaacacag caggtcaagt cgcagcatat 4080 ttgcaggcga aagaagatga attctggttc atggaatcaa aaacaaaggg gcgataatga 4140 ctcgctatga gcaaatgctt atggaagagt tgattcgtaa tactggtgga agacgtcctg 4200 tcaaaaaggg cgttctctac cacattattg ataaactctc gatgtgtttt gttttattta 4260 ttctggctac tatcctgaaa ggagtgtttc tatgagaaac ttagcagcat ttaagttggc 4320 atgtaatcga gcacagcgtc gtgaagaagt tcgtgcaaag tttgacgatt cagagatgtg 4380 catcagcgtt ccaactcaag tgttgcttgt tgccaaagaa cacatcattg atttggagaa 4440 gcgtgttgcg gcgttagaat tagagctgcg cagcaaaggt gtgtttgtga ttgaggatgc 4500 attactatga aactttggaa agaaaaagat ttattttaca gaatacacgg tgcctttctg 4560 ttctactgga ctggtacagt gtgggcacca tcaatgcgtt caattatgag cttcacacaa 4620 agcacagaca aagttctgat tggcaataat ttcaaattaa ggtaacaaaa tgacacgaag 4680 cgggatgcgt gttgtcccga ctcgtggaaa cacactgtca ggatttaccg cgtataaagt 4740 gtacgagatt atatccggca ctggtgaaac aaacatgagt tcttctgtga tgtattctgg 4800 aataatgaag cattcggaga actctgcaaa tgtagttgat gatgaaggaa caattcgttt 4860 catcaccatg agctatttca gagaattcaa acttgacagt ggagtattgt tccaatgaaa 4920 cgttgtccaa tctcaggtag aattctgcca aaccgaatca aagttggtga caaagctgta 4980 tatatgtcgc caacagcgta tgacctgaca tacatgcgtg aatacacagt tgttgcaacg 5040 cacatgtcca aagaccaatc ccgaattggt gctgaccact acatcaagcc attgaaagat 5100 ggtgaattca ttattgttga tgatgttggc gaccgtcgat ttgcagcact cgatgctcgt 5160 tcgctgggac aatgggagaa acttccaaaa tgaagtatta cgtagacgag cacggcattg 5220 tcgtgttctt taaataggag agctgccatg aatttcggcg gaaaagttaa atacatcggt 5280 gacacaacca gtcagttcac ctatggacgt cagtatccgg ttgttgctgg atatggtgat 5340 ggtgtgccac gtcaagatgg acgtattggt gcattcatta aggatgcaaa cgtttttgtc 5400 attgaagata attctggtca cttctatcag ggctctttgg catctggtaa ttggaaggca 5460 atcgatgagg gagtgcaaac atacttccca aatgagcaat taaatccaat ccccgcaagt 5520 cgggcaacct gctagttgct cctgtggttt ttaacacacc ttgcaaattt gttctcaact 5580 ttcgtatggg tttctgagcc agtaccctgt gaattcatta atcaagaagt accgagaaca 5640 attgcaggcg tgacattcgg tgtttgggct tcggccctga gccaattcac gaaaaggtgg 5700 tacatatcaa gcttaatccg gcaaacgtga ggattccgat ggctcaataa tgcggttcga 5760 gcattttaat ggtgtgttca ccatgtttga atagaatagt ttttgtaatt gttttcagtt 5820 attgaaatca ggacacgatt cctcagaatt tataactcta agcaattacc agatgttatt 5880 cgggattagt tgtgtcatga attcatgtaa ttggaggatt tatggcacga gttttgaatt 5940 atgcaaagac gaaaggtgtg cactcgaatg cagtgtacat tggtcgttca atgagaggca 6000 ttgaaggaaa tgcaaagttt cataatccat acaaaatgtt caaagaagag cagcgggatt 6060 atgtttgcga gcagtatcgt agacaccttt ggaatcaaat aaaaacaggc gaagtaacaa 6120 tccaagattt gttggagctt gatggtaaag atttggtttg ctggtgtgct ccagcccgtt 6180 gtcatggcga tacaatcatg caagcaattg aatgggcgaa gaagcaaagc tgaattaaca 6240 aggtgcattc aatgagtgtg cctgattaat tcaacaatag ttggatgtga aggaatttcc 6300 tgcaatgaat tgcaacagag tagtaactaa cttaacttta gagaggcagt aaatatggca 6360 cagtatgacg ataacatgtc cggcatcatc ggcaaaaatg actacaaaga aaaggacact 6420 catcctgacc aacgtggtcg ctgcaagatt cgtggaatct ggttttggat ttctggctgg 6480 gacaaagtat ccaatggccg cagtttcacg tcgttggctt tcactgaaat gactattgat 6540 gaagcagcaa aagtggaaga gaagcagaaa gcgcgtaaag ctccacaagg ccaaggccaa 6600 ttcagcggta gtcagggtgc tggtgctcct gcacaacaac aagccccggc gcagcaacaa 6660 gctccacaac aacaggctcc agctcaacag cagccgccaa tggactttga tgacgatatt 6720 ccattttaat ggaaactctc atcctgccag cccgtgacat tgttgcgggc gatgtagttt 6780 actacaaggg tgtttggtat gtggtggaga gaaactatct ccatacacaa tcgccatacg 6840 caaagatgaa tctgctggag gaaggcaaga gaagcaagat ttcattgtat gtgaaatttg 6900 gtttggctca tcctttcaca gtgaaacaac cggtggcagt tatgcaagaa aatcaacaga 6960 aagcattggg tgttgttcag tgtctacaac gacagttgga acaacatatt caaattgaag 7020 aaactccggc agatgctgtt ggtaatgtaa gctttggaac tgcgattaca atcaacaaag 7080 aagttttgtt gggcacatcc cgaacagaaa tgcgtgcatc agcaaataaa ctgttggagc 7140 tggtcaaaaa gctttagtag ttaacaaaag catcttgtaa tgaggtgctt tgattaacaa 7200 ctggagaact tatatgaaaa ttctttatcg aattgtcaat gctacgttct atttggcatt 7260 acttgtttta gcaacactat gtttggcctt tattctggca actgcaacaa aggcagcaac 7320 atttccgcga actgcggcat ttgattgtca gggtttcgat gtttcactgc accaagattc 7380 tgcaaagcat ccaatattcg ccagcattcg ccttgaaaaa ggcttgtgga tggaagttcc 7440 tcaagagagc cgtgattacg caatctttat gaacatagac aatggccgta ttgcacgaat 7500 ggggaacaaa cctgacggtc gaatctatct ttccttatac gctgacacca atatggccca 7560 agcggacaaa tcggagcacg acttgatatg tcgaaagatt tagagtacca acaaatttgc 7620 aaaacaatct cgactattga ttccgggcag atgcaggcaa acttcttgca ggtaaactca 7680 atggttgggt tattgaatgt ggaattaact gaacatgaat tgctcaactt gttgcacaca 7740 gttgcggcaa gaatgcgctc agaagagcgt gtgagggcac tgcacgagtt ggaagggaaa 7800 tagaccaact caaacaacca acctccaaga gggattgaat ttggatatca aggatttacc 7860 aaaatctgtg gacaatatca atttgcaggt tcaaaagcag caggcgtata tggatacatt 7920 aacctcgcgg ttccaagacg ggcaaaccga aattgaagtt tacacagaag acccaggcgt 7980 ttggactatg gttccgttgc aagccttttg gcagttgcag caggacaaac ttattgctct 8040 gatggaatat cagggtattt taaacagtgc gcaaacgaca ttgcaggtaa agttggatga 8100 aattctgact ggcgttgtcg tgccaccagc aagcaagatg taatcaatgc tgtcgattca 8160 agaaatacct gatgcaattg caaaagccac aaaggcggtg gataaactca ccaaagatgt 8220 ggcaattgtg caagagtgtg tacacgaaac ggccgatgtt tgtatgcaaa cgcacacagc 8280 agatgtttgg ttcaaactcc caacagcaga tgttttgaaa aagctgcggg cagatttgga 8340 gaaggccaaa gccgagctga aaacactcac cgaccttcaa aacctcctca acacaacgct 8400 gtccgatgcg ttgaaaggaa agtaaatgac ctatccttgg attgaattag gcaaatcgtt 8460 cattggtttg agcgagatta aaggcccgaa gcacaatcca gagattgtcc agatgtggaa 8520 agacattaag cgtggcggca tcaaagatga cgaaactcct tggtgttctg catttgttgg 8580 agcaatgctg gaacgttcag gcattgtttc atcccgattt gaatcagcaa aatcctatct 8640 gacatgggga agaccaatca atgagcctat ttatggttgt attgttgtgt tcactcgcga 8700 cggtggtggt catgttggtt ttgttgtcgg taagacagaa tctggaaatc tactggttct 8760 tggcggcaat caatctgatg ctgtcaatat caaagcattc aaacgtgacc gtgtcactgg 8820 ctatcgttgg ccttatgagc ttgggagtgt tcttaaagaa cttccaattg gtgaggcgca 8880 gctttctcaa aaggaaagtt gatggcaagc caagaaggaa cagaggtatt cgaactaacc 8940 gatgaatatc gtaggctgca atctaagtgg aacgaacata aagagttgtt cgactggatt 9000 gtccaacaat acggtgatga attcggtgtt gtgcctccca cagcaacagt tgaagtggca 9060 attcttattc ctgctcgtgt tcggaataca tcaattcatg cgcaagcact tctgagtgac 9120 ttgcagttgt caatggagat aatcgacaat cggaaattgg tgttagacca gctaatcacc 9180 gatgcgaata actccatcga ctacaaacct taacaacatc gcggagaatc cagatgtcta 9240 tcaaagatat gaagccattt tcttcaaatg aagtgcaaga gaagttagat gcaactcaaa 9300 cgcacctcaa caaagcaatt gcagttattg accacctcaa taaatatgaa ggtgatggtg 9360 aaatttggtt gtatattaaa aatcactacg ttccactgcc tgttaagcag ttgattccat 9420 taatggaaga gcggcataaa gaacttctcg cacatcatca caagctggcc gaagcgaaac 9480 gtgtgttgga tgcaaccctg aaaggattgc tgtccgacta agttgggcaa gtgaaagaaa 9540 atcaattcca gaggacacca tttatggcag tcttgactgt ggaacaagtt gtcggctggg 9600 aaacaatagc ccgacaaatt gaagcgttac aaaccaccaa atcaattaaa attcgtttct 9660 ttgcccttgg caatgggact gatatcttaa ttgacggtgt tgaatacaat gctttcggac 9720 aggatatgaa aatattcctt gaggaagcgt gcgaagtacc agaacattcg gcggaactgc 9780 tgtccaagag ctgggaagtt gttgggacag atggcttcgc agaagatttg tgcttgaaat 9840 caaatggaaa gtttgactgg gatgaataca gcatcatcaa tgatttgctg tgcactcaca 9900 gtgaggaact cttaactgct gcaaaagctt gtggcattag ttgggaggga atcgaagata 9960 agtaccaagg tcagtacgac acattccgag tgtttgtgcg ggagcgatgg gacgaactaa 10020 atctggaaga agtcccagag cacgtccgac gatacatcga ctacacgtca attgaagaag 10080 attggcacca cgattaccac tttgagaatg ggtatgtctt ttcaagctga gtttttggaa 10140 gcaggtacgc tgattgtcca ccagccaaga aatcacacgt atgttttatt atttaaaaca 10200 atggtgaaga aggcagaagg tggttgggag tacggctgga tttacaagaa ggtggaaaac 10260 attcgagtaa tccatgatga agttttgttt gacgtagaag aggaagggga attgttcaca 10320 agagcatttt cactctttga cgacgactgg aaactgactc aaacaccata gttccaagct 10380 gtagcatcga agtgccaaac tgtgttggat tcggcattag tttggcggct tcgccgcttt 10440 ggcgttagtt gaaggtgcag ccagtcatgg cctttaaggg agaaatgcat tatacacctg 10500 tgcgcaaaac cttgtcaagc attatttttt aaattatttt tattttaaaa gaataaaata 10560 tttcgttgtt ggctctaaac aaattctgat taagtttgtt taaaggcagc aatgttgctg 10620 tcgtatcctc aagcgaaagc tgtgtgttga cgatactgtg gatatagatt tggacaagat 10680 tctggagctt ttggggctga ttgaatagag tgaacgtgat gcgttggact cagctgaata 10740 cttaccaaat ttttgataaa tgtgtaaata catctggatt agttgaataa ggaaaggtca 10800 agccttttat tctgttcagg aactaattct gatttaatat tagttggagt tggaatggtt 10860 aaagttctct caggagggaa acaactctat ggcgaatgtg aacgttgcgg tactcaagtt 10920 tcttatgtct tttcagattt aagaatgcga cacacgacac atatgtccag aggtttgaaa 10980 cagtatggtg catatcgtta cattgagtgt ccaaactgca aagagaacat tcgctacaag 11040 gtatgattgt tcttatcgag ttgtcaccag tgcgtattac aaaagccacg taagttgatt 11100 gcttgcggtc ggacatggga gtgagttact acttaaggca actcttgata ccgttcgttt 11160 aggaaattgt gacaaaggct agtcaccgga gcattgactg tggcatcgtg ataaggacaa 11220 ttcgtccacc agcgaagcac tgtgctgttt tatgctgtaa ccaatatgat tgtgtgggtg 11280 aaaccttgcg tgtctctgtg ttgttcctgc gtgtattggc ttgatgcctg cgaagccgca 11340 tacgacacac gagttggggc gcaagacgta tcccatacaa tcaccttata acaaacgccg 11400 gagcgcgcag cgtagcaacc agccgcgctg ttgtcggttt gtgtgctgcg gccttgccta 11460 catgggaggc tgtaatcagt cacattatta atttggagta acttatgata gaagttacag 11520 gacgcgttat tgaaagaaat ttcgaagtaa ctcgcgttga gcgcagtaca attcctacac 11580 atcttgcaat taagtcctac attccagatg tgagagaaga tgtagtcggt agaatttctt 11640 ttgacatctg cctcaaccac ttcggctcgc cagacgtcag gcattttgaa cgttttgtgc 11700 gtgcagttga actggacttg gaagaaactc ttggcaactg tcgcaaagct gaagttggaa 11760 gatacatcaa ccgaacttta tgggatgagc tgatgaaaga aatgtaccaa cttcgttccg 11820 aaaacgcaat tctcaaggga caaattccaa aatgaagaat cgtgcaagaa caatccgctt 11880 aaccaacaga gcaggtgaag aagaagccta ctttgagtgt ggcgggaagt ggttcttatg 11940 tggatgcgag cttgttggac ggaaattcca ttatttccac ccttcggtgg ctcagacaat 12000 cttgaagaat aagattgtgg gatttacaat cgaaacaatt tccggactgt gggcaaatca 12060 aacgccgaag cagttgctgg cgaaatcatt tattcgggtg tgggaatcat gaaaaagttt 12120 ggcgtaatta acaatgaaaa tggttacaag tttgaagggt actctctgga agaaatcctt 12180 acagacatgg ttcctagctg ggacatgaac gcagttgcag ttggtgcaga cttgtacgac 12240 tacacaggtg acctgtcaat tggcccgaaa atggtcaaag acctttgcga acacatcaaa 12300 gaattggagg caaaacttgc cgcgcaaaac tcttaagcag aagctgaaaa gccaactgcg 12360 tctggaaaag aaagctcgtg aaatcgagca cgcacatgcc ggaacaacca tcaactttga 12420 attgcaggct atgcagtcgg ttgcaatggg gcacggaatc atggctggac ttcttcatgc 12480 gaagcaagtt cttggtgagt attccgagca ttacaaagcg ctggaacctg tcgcacaaga 12540 gcgcattgct cagtgtttca tgcaactggt tcagatgggt attccccacg aagtagcaca 12600 gcaacgtatc aatcagggac actaacatgg cctttgagca agataaagaa cttgctcttg 12660 aaactgtcaa gatgtggttt cgagaacaag acgccgcata cttggcaaaa gaggacattg 12720 tagttcattg gaatccattc aacacagaaa caaagcgtgg ggagtggacg aaaatacgtg 12780 ttcgtgaact ttgccgaatc attaaagcaa ctcgcgctgg ctttgctgca atgcgatttg 12840 ttaaaccaga agtgattctt ctggcagcgc aggaagaggg acgggcatac cgacaaggtg 12900 tgaaatcgcg ttccactgtt ccaccagagt attttaactt tgagcgtgct ggacacttca 12960 acaacttcga acttttgaca ctgtgcgttt tgcagacatt agttggaagg ggaatgaaca 13020 tggaggcgca ttgccttgga accttgatgg attatgtctt caagaccaaa ggctacgttg 13080 tcccaagcag aacattcaga tggaaactta ttcgtgctgt tgctgacgaa gctggcatca 13140 aagttcgcga ccgacttgac cgcattacaa tatacggtgt tggtcgcttt gttgctgtgc 13200 aaatcgaagg tattaacgac acgattaagc tccatttgag cgaagaagaa ttgaactcaa 13260 tcactgccga agttattttg gcgtttgata gaatttaatt tattctttgt tcttcgtttg 13320 tttgtaatat tgcatcttac ttgaacatta gtgggatgtg attttttaaa agaaaatagg 13380 gcgcattgcc caaccaatcc aacataagga aagccaaaca tggccaaagt agtaacagtt 13440 acacaatacg aagcagtcga cggttcactg ttcctgagcg aagcagagtg taatgctcac 13500 gagttcaagc tggaaaatgg cgcgcgcatt gaagctgccg cagaagcgtt tctgaacacc 13560 accaaagcaa tcgaccgcag ccgtaacatg cagggtaacg taatcgcgaa cttcctcgca 13620 ttctacctgc cgtgggttga agctggttct ccagcagttg aacgcactgt gttcgatact 13680 ccgaaagctg aaaaagttga aggcgcagaa ggcgaagctg ctgctcctgc tgttgcagaa 13740 gtcgctgccg atggcaccga agagcaacca atgttctaat cgatctggct ggtctgctta 13800 gctaatagcg acgcgccggc tttgtaacgg catctcacag tagcactgcc gttgcaatta 13860 tcaaggtcaa ccacagccaa aaggccaaga gttgcttgat gtgacgtcca ctcattccct 13920 ttatggtaag cctgagtggg cttcgctctg tgtattgaat atcagtatgc atagtgaagt 13980 ttgccctgag tatggaaata aactgctcat aaatctgacg tagttcacaa aggtggatga 14040 aaccagattg cctgataact tgaattagcg ggaccatcaa tcccatgtcg gccttgtgcg 14100 ttaacaggcg ctaatagaga gtttgcaagc ctcgcagttt tcagtctgcg ttatcaaaac 14160 tgtccaaaca atgcacatgt cgaatagcgg aaagacgatg gggaactacg caatcctcag 14220 tgtgcaccaa acatcgacag gagccgaaat caaaggacag gttctgcatc ctccaaagtt 14280 gcggaagttg ggttcgaatc ccaatcaggt cgccaaacaa tggaatgaaa gtaaggaaca 14340 gtacctgtaa gtccgtgttc atttgcggct ggagaaaagt actgaagagt aattccacca 14400 aacaatgggc tagtgcaaat gtcagtcaca gaaaaaaggt cgtcaccggt tccccgactt 14460 tgtatgtgca aatgtcagtc acgtgcccac caattcactg aggccaataa tgggtaagga 14520 aggacgctac ataaacgacc caaaagatgg tgtcggcgct cacttagatt ttggaataag 14580 tgatgacggc aaaactgggt acgtttacaa tggcgcttgg tacttcaaag tcttggaatt 14640 taatcctcca tacatgaaga ttaaagtgga ccacccaaga gcagcacctt tcgtggagta 14700 cttcaatgtt cacaaagctt aaaatactcc ttgcgggcat tgtcgccatt gcgggggcaa 14760 ttctggttgc atttctgaaa ggacattcat ctggaaaaga ggaagttcag gaggaagtgc 14820 gagagcagga aattaaaaat gtcgaagcag ttgcaaaccg gcgtgttgaa accgcacaca 14880 aagttcagaa agttcagcaa actgtcattg cttctgatga caagtctgtt gacgatgagc 14940 tgctcaaagg gtggacacgc ccccctggta ttaaataact actgtgacat tgccaagcca 15000 atttatctgg ctgtggaaga aattcctgca atgacgatgg aaacaaaaag acaaatcctt 15060 gtccacaata aaacgtgggc aagtttgtgt acaacacccg acaaggtgaa gtaggtcgca 15120 actgcggcga ccttaatatc gcaggatgac ggacgtctat aactcccctg tgagtcgagc 15180 ctgttgagac tttaaacaag tgcagaagtc aacctatgac ttgaaataat gggctgaatc 15240 cgagtggctt aacgtcggat agagaacaga ctcttaaaca gttgcggtaa ctcagtcaga 15300 ccaaagaaac tttgctgatg acagatacgt cataaacatc ccagtgcgtg gaagcgcaat 15360 tggaaataaa cctactcaga gcaacctgag aaaaacaagc atgccgaccg aaatgtcgtt 15420 aaactagcta aggcggcgtc actaaaggga cactaacaga ggttcgattc ctcggctgcc 15480 accaatgggt gcatgtcaga atggttatgt ggcgtattgc aaatccgctt atactggttc 15540 gattccagtt gtaccctcca aattcgctgc tatcatagca caggaagcac atcagataat 15600 cttatcaagc tcgtcctgat gactgtgttg gcagcaaatc aaactcctga tagttgtgca 15660 tctattcaaa atgcaaacag tgctctggca tactggtgtc aaaaccagac aacttcggtt 15720 ggtggcttga ttgggttgtg tagaaatgga atcgaaattc ctgttgtgtc tactccttaa 15780 cacaagttgg cttggttaat cacctagcca tgttcctatt cccatgaaat atgcaaggca 15840 gggttccgca agatgattag aaattgcggc aggtttgacg atacctgtgt tagtctccca 15900 taatgcaaag ttggaaatct ttggccgata aattccaaat ggttcgctgc tccattcaaa 15960 agcagcaaaa ttaattgagt tcatttgcac agtggcatat tacataacct gcaagcgaag 16020 tacataacca aacgaaccac gcgagtgttc tcaattaatt ttaaccaaac caaggaacct 16080 ttatgtcaaa catgattcct gtttcaggaa aagatttagg tacaatgcgg gatgaactcg 16140 cgaagattgt gttctcaagt gctgttgtgg gcatcaaaga ggtattccac tgtccgcatt 16200 caaaagagcg tcttgacaac ttagcagtcg gcattgctaa agtttcttat aaatttgctg 16260 atgcaatgat ggaagctcgt atggtgcaac ctacctcggt tggtgttgac gtacctcaat 16320 ctgtcttggc cgaagctttc aataagggct gaaatgagca accaaatcaa catcgaatta 16380 atcgaccaca tgggcaatga cttaaccaca gtcaatgctg ctcgtgtgtc ttatggtgca 16440 tggtcaacca actttggcga ccgtgatgca gctcttgttg aattcttggc agagcacaag 16500 catgtaacac cattccgcca cgcacaaatt acgttgcgat gcaaaggccc aatctttctg 16560 gttcgtcagt tgggaaaaca tcaaactggc tttagctgga atgaattgtc ccgacgatac 16620 aaagatggtg atgcgattga agttgaatgc tatgtgccgg aaattgttct tggacgtcca 16680 agcaacctca tgaaagaaac agcacaacca ttgccggatt ctgattgcga gaatgcacag 16740 gagtttatca ctcgggtgaa caatcaagca attgcaaact atgaaatact tctgcgcaac 16800 ggtgttgcac cggaacaagc tcgcatggtg ttgccacaat ctatgctcac tgagtggatt 16860 tggactggct cactgtttgg ctgggcatct ctttatcggc agcggagcaa acttaacgct 16920 cagttggagg cgagaatttt ctccgagaag ttggacgcaa tcatgtccga gcttttccca 16980 atctgctgga aagcattaat caaataagga acttagccag atggttgttt cgcaatgtgt 17040 tgcaaaaata aagcacaact caccaacttg taaatccaca tcaggcaagt ctgtgcaggt 17100 gtggcttaat gaggatgaag gcgggaagcg atactactct ggcttttgtt tcgcgtgtgg 17160 ctgttctgta ccgaacccgt atggagacaa tccaccaaat ccagaagaaa ttcatgtcaa 17220 gactccagaa gaaattcagg aggaattgga tgaaatcact tcctgcaaag tctttgacct 17280 cgaccaccgg agcattgagc cagaatattg gcaagctgct cgtgttcgtt tgctgtactc 17340 cgagtatgac ggcgttactc cgtatgcctt ggcccacgga tacacgaaga acggcaaact 17400 tgcaaggtgg aagattaaac tcctgaacaa aaaggttatg tggtcggtcg gtgatacgaa 17460 agacaacgac ccttatggct gggagcaagc caaaatgtgt ggcggttcta ctctctatat 17520 tacagagggt gaagaggaca gcatcgcatt acggcagata atgaaaacga tggcacttgg 17580 aacagagtat gcagcgcttg attactcggt aatttcgctg acagacggtt ccgattcagt 17640 gcacaaatct ttggcgcatg ttgccgaaga aatcaaacag cgttggcaga aagttgtcat 17700 agttttcgat aacgatgagc caggcaagaa agctgcaaaa gaagcagcaa gacttttgcc 17760 cggagctatg attgcacaac tccctgcaaa cgacgcaaat gagtgtttga aacgtgggct 17820 gcttaaagca actcgcgatg cagttgtatt ccgtgcagca agaccacttc caacagcgtt 17880 ggtcaataaa gacctgttaa tgctggaact ggatgaagaa gttccaatgg gtgtatcaac 17940 tccgtggccg aagttaactg atatgttcta tggtcagcgg cgtggagaaa ttataacact 18000 tggcggttcg gaaggtggtg gtaaaaccac agtagctcgt cagttgaatg agcacaacat 18060 catcgagcac gattggggtg tgtttactgc gtacatggaa gaaactccag cagaaacaat 18120 tcgaagaatg gcaagtttgc acgacaacct tccatacttc aatccaaatt tcaaagatga 18180 cccacgttat gatgaagaga agttccgagc aacttgccgg aagttcctcc ctaacatgga 18240 gatttgggac aggaagcagc aaggtgaaga cccatatgaa acgtgggaag gtattaaaac 18300 aatcctgcgt caaattgggc ctgacattga catgtttatt ctggataacc tgacgatttt 18360 gtcggaaggt atttcagcgt cagagaagaa tgactttctt ggcaaacttt acgcagacca 18420 agttaagttg gcagaccaat atcaattcat gattttggat ttgtcacacc tcaaccctgt 18480 tgcaaaaggt cagcgaccac atgaagatgg tggacgaatc aagaaaggag actttactgg 18540 ttctcgtgca gcgtccaagt attcgcacgc tatgtttggt ttcgaaagaa actcacaggc 18600 ggttgaccca aactgttcaa taatgcgttc aatcaaaggt cgtgcatctg gtaaaacaga 18660 agcattcaag acgtattatg aagccgaatc tggccgaatt atccagcgtc attgggaaga 18720 ttccctgttt gaaacgaaag aaattgttac attaacaaag aaatctgggc ctcaccagta 18780 atcaagtcca gccatcctac tgtcgtgaga tagaagcttt ggctgcactt ccaaagtatg 18840 gagccataaa gtgttgtatc cttacgaacg attgtttgcc agtgacattg aatctactgg 18900 tttactgcac caaatgaagc gacaggaaaa tcctcgtctg cacaacattg gagcaatcga 18960 cgcactaacc aaagtcgaaa cttgtattga gtggacggaa cgaaaacgta ttcagacgtt 19020 tctggattct ggaccaacac ttttgatgca caatggttat tgctttgacc atgaagcaat 19080 ggtgtttctt ggttatgaca tgtcaaaagt taccatcatt gatactttgt ttatcagttg 19140 gtatctgcaa cctcgccgac tcaagcacgg tcttgaaggc tatggtgaag aatatggtgt 19200 tcctaaacct gcaattgaag attgggaaaa ccaaactcag gaagagtaca acttccgtgt 19260 gatgcaagac tgccgtattc aactgcggtt gtgggaagaa cagtattccc agctactccg 19320 catttataaa actccgcaag aggtaaaacg ttttgttgat ttcttgatga ccaaagcgcg 19380 tcagcaacga atccagcagc gtactcggtg gaagttgaat atcgataaag caattaaatt 19440 ccgcgattct ctggaaccaa tgattctgga gaaaaccaca gaacttgaaa aagccatgcc 19500 agatattgtg cagtacgcga ttaagcctcg ccctgcaaaa tgccacaaag gaaatggcac 19560 tttgtctgta gctggtatga agtggaaagc aatgtgtgat gcaaatgggt tggattggaa 19620 agacccagat ttgcaaatca aattgaaaac tggcaccaag cctgcaaatg ctggtagcca 19680 tgttcagctt aaagattggt tgttcagtct tggctggaag cctcagacat tcaagtttga 19740 ccgcaacaaa gaaacaggtg aaacccgcca gattccacaa attaccgtca aagatgaaga 19800 tggtaatcct gagatttgtc caagcctgca tgaacttgca gaggaaaatc cagacgctgg 19860 aatccatcat ttaattggtt tgggggttta caaacaccgt ctgtctgttg ttaacggatt 19920 cttacgtgat gttgacgacg aaggattctt gactgcacgt tgcggtggat tgacaaacac 19980 actccgactc aagcataaag agttggtgaa tctgccgtct gttcgtgtat ttggtggtgc 20040 cgaacttcgt ggtttgttgg aagcatttag tccagactat gaagaacttg gttctgactt 20100 gtgctcactc gaagaccgct gcaagcacca ctaccaatgg gattacgacc ctgaatatgt 20160 gaagaaacag ctcgcacctg attatgatgc tcacttggca ctgggtgtta tgggtgggtt 20220 tatcacagag aagcaatcgc aagaccacaa agataagatt gcgaaggttg ctcaacgtcc 20280 gatgtttaag tcaaccaact atgcttgtca gtatggtgct ggtgttccaa cagttgcgcg 20340 tactgcaaag tgcgaccaag caacaggtgc ccgactccat aaagcgtatt gggacttgaa 20400 ctggtcaatc aaagaaattt cggccaacac gattgtcaaa acaatcgatg gtcagatgtg 20460 gcaacagaat ccagtaaaca aattctggta ttcgctgcgt actgagaaag accgtttctc 20520 aacattgtgt caaggtactg gcgcatacgt gtttgatatt tggtgcaata acgtaattgc 20580 aatttgcaat gaacgttacg gcaaagaccc aatgctggct gggcagttcc atgatgaact 20640 tgttctgcat gttcgcaaag gtcatcgtga attgtggact tctattctga aagaagcaat 20700 gcaccggaca aacgttgaat tgaaattgaa tcgtgattgt gactgtgacg tacaatttgg 20760 cgataactac tcagagattc actaatgaaa tttgcaatag ctttaatgga cactcatgag 20820 aatgagttgt accacttcca aaacttatct gagcaagccg aatcgccaac tggcgcagtg 20880 ctaagatttg ttgcacaaaa ccaaactaaa ctgtttggcg agagtaatgg aaactatgtc 20940 ccgtttgtgg agaaggtttc tgcgacaaca actgttgaag aactggtgga agaactttcc 21000 acgtgggact tcattgcctc tatcattgaa attaaatagg tgcacgtaat ggctttaatg 21060 aaaacaccaa ctcaggcatc gaacaaagca ccttcttccg caccactgtt ggaagctggt 21120 ggttatccag ctcgcgtatg ccggattatc gacttgggta aacagaaggg ttctatcaag 21180 ttccctgaac ctcagtacaa aatgctggtc acgttcgaac tgctggaaga gttcatgcag 21240 gaagttgacg ctgacggcaa agtcatcatg attcaagacc ctgatggcga gccaggcgaa 21300 atgattggaa agaacattga agacaaaccg cgttggtttg actttgaatt ctcttacaat 21360 cctgacggct tcatgggcga caactcgcac atctacaaat tcatgaaagc agtcgatgcg 21420 ctggaagtta aaccaaatcc agaacagggt attgttggtc atgaagcgaa gaatctggtt 21480 gacctgttgg gcgaaccctt gactgtcggt ctggtaactt acaccaaaca gggtggtaag 21540 aacgccggac aaatcgcgaa taagattgcg accttctccc caatgaagtc caaagagaag 21600 cgtgatgctc gtgcgttgat taacccaact ctgattttcg atttgggcga ccctgatgtt 21660 gaagtgtttg gcaaactgcc cggcggcgat tccgaatacg caatcaagaa ccgcattctg 21720 gcgaatctgg aattcaatgg ctctgctctg caagctgcac ttggtcaaaa gccaacgcag 21780 gaacctgtgg aaaacaaagc cagtgaagag caagtcagtg ctgaaatggc tgctgaactt 21840 gaagctcaga aagctgctcg tgaagcacaa gctgcgcaaa atgctgccgg tgctggcgac 21900 atgccgttct aataaataac aagtctattc catgttggat agacttactc actttgagta 21960 aaggctgtgc cacaattggt gcagccttat tttgatttgg agttttgcga agtgtcttta 22020 aataaaaaac gagttcttct tctggttgat ggagatatgg tggcattcag ccattgtgcc 22080 gcagaagaat atggcaagga accggaggac attagtttcg caaagattca aatgtctatg 22140 gatgcaaaga tgaagtacat gcacgaacgt ttgggtgcaa ctgattctta ttgcctgatt 22200 tccggcgaca caaacatgcg ccatgtaatc tcaccagatt acaaagcaaa ccgtgacggt 22260 gtgtggcgac ctgagaactt gaagaatgcg aaagctcatc ttcttgttgc ttgggaaggt 22320 ttgaaaatgg acggtctcga agctgatgac ctgatggcaa tgttagcccg acatgactac 22380 gaaatggtca tgggtaaacg taatgttatt aaagaactta tttataaagg gccatgtaca 22440 tatgacaaag tcattattgc atcgctggat aaagatttgc ggcaagtggg taaactggtt 22500 ccaactggaa acgggccgga aattgtccac taccaatggg aaagaccatc acaaaatatc 22560 ggtgaaaagc tcatccctgt ctccggattc ggggagctga aatgcatcat caaagacaac 22620 ggcaaacaga aaaagaaaga agttaaaggc aatggtccga aattcttcct gtggcagttg 22680 ctcactggtg acggcaccga tggtgtcatt ggttgtggtg ttcgtgaaaa gaaagtttac 22740 aagactggcg caaaagctgg tcaagaatac atgaagcgtg atggtgttgg tgcggtcgaa 22800 gcattcgaag cactgaacaa atgcacgtcg tatgcagaag ggctgcaaat tgtggccaat 22860 cattatctga tgcggtttgc tgatggatgg caagaacaac ttctcaagaa cggccgattg 22920 ctgtatatgg caaacatggt ggatgaaggg cacttggtgc gattgtggca ctataagggc 22980 ggcattgtcg accacttcga tttgaaaaca aaacaaatcg ttttggcatc tgcgtagaca 23040 ttgagactct gcacctctaa gaattagtag aaggtgatgg aatggcttat ggctattgcg 23100 gaacggaagc aaagttcaca gcttggctgc aatctgcatt gcggtcaatc tggagcaaac 23160 atcctgcaaa actttctctg ttagagaaga agcgggtgcg tctaaaggtt ggcacgacaa 23220 acaaaatggt gttccacatt cagtgtgagc actgcaagaa attgttcaag ctgaaagaca 23280 ttgaggttaa ccacaagaac aagtgtggtg ggcttagtga actgagtaag ctgccagagt 23340 ttgtcaccaa cctgcttctc gttcaagaag aagatttgga attgctgtgc catgaatgtc 23400 acggcattgt aacttatatg gaacggtatg gtgtttctag gcgtgatgca gtgatagaga 23460 agagggttat tgctttctca aagttgcctg ataaagagca aataacgaag tgcaccaaag 23520 ccaaaatact acctgttccc aaaacaaaaa tcggacgcaa aaatgctgtt cgagaatacc 23580 tgagactgaa tcttaatgac tattaagctc ctcgacgatg gcacatcgta tacaaaggca 23640 ccgcgcatg attgggaaga cccgaactgc acgctcaaag gtcgttgcac gttttacatc 23700 aaagttgacg gaatacgagc tatccgtaat aagagcggtg gtgtgtggag tcgtaactcc 23760 aatccccttc cccacttggc tcaccttgag ttccgagatg ctgagatatt ccgtggtagt 23820 tggaatgaga cttcaagcat cctcggtcgt attgacccgc cagccattcc tttaactcag 23880 gaaaatgttt acgagctgag tgacggcatg gtggatgaac gcctgtacct cggctgggca 23940 aacaacccaa gcaacgagaa ccttcacaag ttgatgttga agcagcttga gcttggtcat 24000 gaaggcctca tcgttcgttg tgtggataag aaaggtcgta tcctatggtg gaagattgta 24060 ccatataaat atgctgatgt gaagattacc ggattcaaag aaggtactgg cgcactgaaa 24120 ggtatgtgtg gctcaatcca aactgcgcat ggtgctgctg gttcaatggt tgcagattgc 24180 ctgcaagatg ttggtgttcc taaagagaac ctcgcaatca ggcagtggct gtgggcaaat 24240 cgacacacac tgcttggctc tatcattcag gttcgttatc gagaaaagac ggaagctgga 24300 aaactccggt tcccaagttt ggtgcgtttg cgcactgaca aatcagaaga gagtttcgat 24360 taatggcacg agttcttata gaggatttgg gtgacgagca ggttttcaag tttttaatac 24420 actcgccaaa tcagaagttt aaatatttgt cgcatgacga caagaaccag agagtaaact 24480 ttgtgcctct ggacttgcac ggcaatgttc aaccaaactt cccttcatat gcatattatg 24540 gtgcggaggt tgaggtacta tgaaaattgc cgtgatttta aatggcccgc cgggctgtgg 24600 aaaggacaca attgcaaacc tgttttgtga aagtggttct ttcatcaagc atcaatttaa 24660 ggatgcactg tatgaacaca ctgctaaaca ttttgaagtt gatttggata agttcatcca 24720 tttcgccagt gaccgtgacc ttaaagactc aacgtctttg gccggtttgg gcggaagaac 24780 acctcgtcaa gcgctcattt atgtcagcga aaacatttac aaacctcgcc acggttctga 24840 ctattttggc aaagtggaag ctgctcgtgt tgcagaatat gctgggcgtg tgggtggcga 24900 tgttaatatc atttaccccg atggtggttt ccttcccgaa attgggtgcc tcgaatctgt 24960 ggtggatggt gttcttctca tacgtttgca tcgtgctggt tttgatttcg cgggagattc 25020 tcgctcatac ctgcacgcag agcacacgga aaagttcagt tccgtggaca ttcaattaac 25080 tgacggtgac atcttcggcg cagtgctgga tgttgatgtt gcaatcgaaa actttaaggc 25140 tggtctgtaa tgtgttgtaa gaaagccccg catgtgaact ttgttctgtg ccaccctgcc 25200 gctcaactgc cgtcatacga aagtcgtggt gctgcttgcg ctgatattcg tgcgactgag 25260 cagaatgtcg taatggctcg tgattcggaa atcttcggaa ctggcctgaa accagaagtt 25320 ccaaaagacc acgtcatgct ggtgttctca cgctccggtc atgggttcaa gaacgatatt 25380 cgtttggcga actgtgttgg tgtaattgat tccgactatc gtggagaaat caaagtcaaa 25440 ctcacaaacg atggtgaaga agactactgg gtaaatgctg gcgaccgtat tgcacaaatc 25500 atgattctgc cttgcccgca agcaacgttt gaggttcagg aaaatctgac ttcaactgca 25560 cgcggcgaag ctgggtatgg ttccactggc gtaaaataaa gttcaattgg agaaaccgtg 25620 agcttaaagc aaagatttga aactctgctg tctcacctca attcagagga gaatgagatg 25680 gcattggatt tactgcaagc aattgaagtt gcagacgaac gactggacaa gttagaccgc 25740 cttgaagcgt ttggcgttga caactggtct ggctatggtg atgcgatgga gtacgaagat 25800 gaataaactc tgcccgttaa atcttggaat cgacacagca gcattccgaa gtctcgcacg 25860 acacatgttc gatgaccctg aggaaatctc agaagaatat gttgaccgtg acgagtgcag 25920 aatgcagtat ctcaccgaga ttgagaactt ggtcgatgcc ggagaaattc ttgaaggtct 25980 aggtcttggt cttgaccctg agattcttgt cggttgttgg atgctggaaa tttctcgcac 26040 cgactccgat ggaattcatt gggacgagtg ctgggaaatc gttcgtgtag aacaagtaac 26100 tgaaactaaa actgtaacgt actggaaacc tattcgtgaa caaaccgcaa ctgaggccgc 26160 gccatacggc aagccaaacc gtagtaaaaa ataaagtagg cgtaaccttc tgttacctcg 26220 aaagtgctgt tacctcaaca ggtggtggca cttacctaac tcgtgcattg gcagctcgta 26280 aagaaaccaa tgcagatgtt atcgtgttcg tgttgccgca ttctgacaac acatctaaga 26340 acgtcgagcg tatcattcgt ggacgcgacg atttaatgtc agcggttcat ccgctaatcc 26400 tgccatttaa aattgtggtg gtagtcggaa ccagagttga gtatttgaaa gatttctatt 26460 cagagaaaaa gtggtcagac attaaactct aatcgcatac gccgaatctg tagggggcaa 26520 tctcgctccc tctctttcaa aaggaaatga cgtatgcgca ccattcaatg cagcgaatgt 26580 gggcacgaaa ttgcagagga tgattttatt actcaacatt gtcctatctg tgattcttac 26640 atgccagtca gtcgcttagg caataaggct gacaagagtt tggagaagat tcttcgaatc 26700 aatccagtgc agccagttgc tcaagaattg gagtaatacc aatcaatggc aagtgaagat 26760 ttgggtacag aatttaacga cacagttata ttctacctaa ccaaaaatta tgggaatctt 26820 ttaaaagttt ctcgtgaacg tttctgtatg ctttccttac cattgcttcg taagtatatt 26880 tctgaacgtc cggcaattgt aagtgagtat gttgccaaca tccgtgatga agtagttggt 26940 caggggctta cagataactc agtattgtcc tccttaatca aagcacaaga ggacagactt 27000 ctcgcagcag acccgaaaga ttcttcggca tatgcaaatg aaattgagaa gatgattaag 27060 gaaggtaaga cagaccgtgt tgctggttct gcacttgagg agccaacctc agagaacgac 27120 aacacgaagc agctcataca tgatattttg tatgcgccat taactgtcga tgcctcatgt 27180 attccaaaga aagttgctga tgaagttcgc aagattctga tgggttcatt ccgtgaattc 27240 tcaaattggg cgttctacct gcaaatgggt ttccagttcc aagaacagga tttccacaaa 27300 gtaattttcc aaacatgtca ggatgttgtt gacggcaaga ttgaccgagt gattattaca 27360 attccacctc ggcattccaa aacacaaacc tgtgcaattt cactaccact ttattcattc 27420 tgccacaacg taacaagtca caatattata acctcatatg ctgaggacgt tgttctggaa 27480 tcaagcggct atatccgcac tgtgatgtta gacccactct tccaacgaat cttcccaaat 27540 gttcgaattg acccgaacaa gagaagcttg gaacgttggg gtacgacaaa gcaaggtgta 27600 atgcacgctg ttccaactgg tggtaagatg actggtaaag gtgctggact tttggcacca 27660 acgtattctg gttgtttcgt cgttgatgac gttattaaac caaaagacgc atactcagat 27720 accgttcgta cagaaatcaa tgaccgatat gacaacacat tcatgtcgcg cctggccaac 27780 gatggtgaaa taactgttga tggcaagcct gtcaaatgcc ctcgcactcc gatggtaatc 27840 atcatgcaga gggttcatga ttcagactta gttggctatc ttcttcgggg cggttcgtct 27900 gacaaatacc attatttaaa tattccggct atagttgaaa gggatactgg ctctgctgag 27960 tggtatgata aactcatcaa gaagcaagga tacacccacg ctattccaat cttgtttaac 28020 ctgaatcgtg aagctgagaa atctgcttta tgggcatctc gcaaaagcct tgaatcactt 28080 gaggcaatga agaaagcgaa cccgtacact tacaatagcc agtatgctgg cgacccaagt 28140 gcacaaggtc atgggctaat taaagaagag tggtggcagg aatatgatgt tgagtcgttt 28200 gatagaagca gaattgttcg cagtttcatt acagcagaca cagcttccac cgacaagaca 28260 tactcagatt attcagcatt gttccattgg ggagttacca aggaacgaga tgtttattgc 28320 ctcggcatca tgctgggtaa atatgagatt ccaaaattaa aagaagaagt gataaagttt 28380 tggaagatat gcaccaagct ggacatgcgt gctccatgtt gtgttgctcg tgcattgcac 28440 atggaagata aatccgctgg gcagttcctc aaccaacaat ttctgaaaga tggttctgtt 28500 cgagttatgc ctgttccgag agatgggaca aatgtgaata acaaagtaac ccgattcatg 28560 aacacaataa cgtatttctc gcaaggcagg attttcttcc cagccggaca tgaacacatt 28620 gaacatgtta agcgtgagat tcttggcttc acttctcttg gctctggtac tggacacgat 28680 gactgtgtgg ataatgtttc cgacatggtt tcaatcgaat tctccggcat gagtgccaat 28740 tattcagcgt ggatgtaaca tggccgtatt aacacggatt gatgctcggt ctgtgaagaa 28800 caacgccatt caggttaaag acttggatgg caatattctt tgcaccatcg aagcaatgcc 28860 gactgaacgt tccgacaatg cagaaacgat gcggaacgaa gttaaccttc gcctttgtgc 28920 atctgacaaa gttgtctttg ttaagagcaa tggaaaggta ttgaggaagc gataaatgcc 28980 tcaacatgat tcacacgtac cacgtcacaa agctcgtaaa gagcgaaaac ttgctgacgg 29040 tttggaaaac gttgtctctg gactgggtgg tgagaaggat aaagcggaac ataaccgctg 29100 gactttctcc aacaagaatg caaactatcg cgaattgatg aaccgcttcc gtgaggattg 29160 ggttagtcag aaagtttgta ctgtaatccc gcaggacatg actcggaagt ggcggcatat 29220 tgattctgag gaagggcgac ttgcagataa aaagctgcgt attcgtaaac tcttccgtga 29280 aggatatcaa tgggcgagac tttatggaac atcttttgtg ttgcttgacc tcaaaggtac 29340 aggcccactc aatactccac taaatcttga taagttgaag aggggatgtg tcaaatccct 29400 gagagttatt gaccgctccc gaatgtatgc ggctggttca gtcgtacttg atgcgttgag 29460 tccacattat ggtttgccag agttctacac tttgtcaggc tattctggaa tgattcatca 29520 cactcgtttc ttacgcttcg aaggtacgga gctaccactg ttcgagttcc aacgaaatat 29580 gtggtatagc gattcagtgt tgataccact gttgaaaacc attgaccaat tctacacaac 29640 tgctgctgct gctgccaact tggcgcaaga agcaactatt gatgtagtct cggttgctgg 29700 gttgcagtca ctcctgacag cacccgaagg tgaacaggcc gtattgaaac gtttccgact 29760 catgaagttg gcaaagagca tttataatgt tcttatcctt gatgacacgg aaaagtacga 29820 aaccaaaagc attgcgctat ctggcgtaaa agatttaatt tgggaatatc ttaaaatcgt 29880 tgctgcatcg gttggtattc ctgcaacacg tttcctttct gcatctccag acggtatgaa 29940 cgctacaggt gaagccgacc tcgtaaacta tatcgacttg ttgactggct tgcagacgaa 30000 tgtctttgaa cctcgtttag atgtgataga caaaattgtc caagcgcact ttggcattga 30060 agagtacacc tatgaatggt gtgatatctt ccctgaatcc aatgttgata aagcgaaacg 30120 tgcaaaagac cttgcggcgg ctcttgactt gctgattgcc aatggcacca tcacaaccga 30180 agctgcaaac tgcattgtgt cccattcaaa ggtatttggt gaatgcgata ttggcaagca 30240 taatccaagt cctccaaaca ctcaaaaggg taatgcgagt gattcgaaac caagcggtaa 30300 cgctaaatga tgaagagtct gttgcatcgg ttgtaatgtt tgccgaccga cagacaattc 30360 aatcagcacg cgtattgcgt gattctggcg aaatgattgc accagtcacg attgctcgtg 30420 ttggtgacat ggcatatcgt gccaaggaac tgggtgtaca gtttgccgac ctgccaccag 30480 aacagattgt aatggtgaat acgccagcaa gtgttctgtt cgacgaagca actctcgacc 30540 tttgtcgttc gatggccgta actgttggac accctgcgca agatgttgac cttaaaaaca 30600 acaaatcttt gcagaaaggt ttcctcgaag ggcgaccaga gccggacggc tctcatctcg 30660 gcggttttgt tgttctcaac gacgcggaca ctattaaatt agtagattcg ggagttgacc 30720 aaacgtcgtg gggacatgac gcagttctgg aacgtgtcga aacaaacggt gttgtctctg 30780 ggtcgtgcac aaacaactcg catcggtgat tccggtgaag agattgatat tgtcgataag gcagtatttg 30900 atgcgctgga agcggagcgt gattctctga gaacaaaggt tggcgctctg gaacaaaagc 30960 tgtcggatga agaagcttcc agattaaccg acgaagcagt gcagaatctg gttgaatctc 31020 gtgtaatcga acgtctggca ttgctggttg atgttgctcg actgggcgac gaatacagct 31080 cgatggactt cgctggtaaa tccgaacaag aaattaaggc gctggttgta tccaaactaa 31140 tggacgcaga tgtttcaggc agaagtgctg agtatatctc tgcacgattt gaaattgcac 31200 tggaagattc agactctgta actttgtctg acgcagtaag ccaatctctt cttcacggta 31260 aagaagagaa atcagaacgt aaaccatctg ttcgtgacga agcacttgcc cgtcgcaacg 31320 aacgcaattc gaaataagga tttcggacat gccaaagcaa gattggacta ttaacactgg 31380 cgaagcatac gctggtgaac aatatggtct ggcaacaacc aactcccagc gactgactta 31440 cagcaccgat gcagaacatt ccgcatatgg tttggctgtc aagcagggtg tggcttataa 31500 caccattgca ctgggttcag atgctggtga agttcttggt atcaccatgc gtgaaatcaa 31560 gctggaatcc aaaactcggc cgggcgatgg caccattctg attccaaaag gtcagccact 31620 tggtgttatg ctggaaggac caattttggt caaactggca actgcaatca ctgatgcaga 31680 tattggtatt tccgcaactg gtactttcgg cggtgttgct actggttaca ccaaagcaac 31740 caatgttaaa gcactgaaat atccggctgc tgctggtgat gtaatccctg tgatgattaa 31800 catggtcggc ccaaagccgt aacaccaccc gcaccaacta ttaccgctgt tgctgctgca 31860 attcctgacg taactggaag tgctggcggc actgtaactt tcgacaagtt gtttaatatt 31920 accaactcgg cgattactga ttatacctat gctgcaagtc ctgcaactgg tattacaatc 31980 agcccgacaa atggtaatgt tatcgtagct ccgggaacgg ctgctgcaag tgtcactgtt 32040 actgcaacag gtaaagctgg tggtgcaact gctggcctga cggctactaa gtcgttcaac 32100 atcgcataac aattggcgtg gcaatgttgc tgcgccataa tggaataaaa gaatgccaaa 32160 gattatcaaa ctggccgacg gttctgaatt cgaactggat gatgctattg cgcgtctgca 32220 agatagcgtt tctgtcacac tgagtgacga cgatgctgtg ttcttccagc gccaacttga 32280 gttcattgaa gctcaaacat atgacaccct gtatccagag cttgaagctc gtgcggcatt 32340 tggtgttgat tcatctggtg gtcctggcgt tacaactctg acgtatcgct cgtacaacca 32400 tgttggtaaa gcacaggtaa tcaacgcccg tgcaaccgac ctgccgaagt ccagcatttc 32460 cggcaaagaa tactctatca ctgttaagtc tgttggtact gcatatgact atgacattga 32520 tgaagttgct tctgctgccg taactggtct gccgctggaa gctcgtaaag caatggctgc 32580 aactcgtggc tatgagcaat acgtcaactc tgctgcatgg tatggcgatg ctgcaaatgg 32640 tttcgttggt ttcttcgagc atccagacat taccaaagca aacgttgctg ctggtgctgg 32700 tgctggtaat ccaaccaagt ggaatgcgaa agctccgaaa gaagttatcg ccgacctgac 32760 cactgctgtg tctgcaatgt acgcctccac tctgaaaatc atgcgtccag aagaaatttg 32820 gatgcctgta gagcatgagc agtacatctt caacaccgca cgttccgacc aatcggacat 32880 gacaatcggc cagttcttca tcgccaacaa ccagttcatc aattcccgcg ataaaatcaa 32940 aggtctgaat gcaatcaaag gtcacggcgc tgctggtact gactgcttcg ttgttatttg 33000 ccgttctgca aatggcaaca agacattccg tctgcgtgaa ccgctggcat tgacttggca 33060 gcctctgcaa cttcatggtc tgatttatga aatcccaggc cgtggtcgct tcgctggttt 33120 ccaagttatg tacccagccg caatctccat caactccggt atttaatcgg tgttttgaaa 33180 caggcgggag caatcccgcc aaattgagag aatatcaatg caacttcgca acaaccaatc 33240 tcgtaacgtg caactgcatt tctggaaaga aaacgaagat accaaacaga aacagttgga 33300 atatgtgcat attccaggcg gtgccaccgt tgaaattgac gacgaaatct tcaaacaact 33360 gacttcgtca aagtctgaat ctcgtgaact acgtgaagaa attctgcgcg ttgagggtgc 33420 tgatgtaacc caccttgatt caaaagaaaa agttggtatc aaagaattct ttgaaaccgg 33480 caaagtcaaa acggttaaca tcctgaaaga agaaattaag gcaggtttgt tcaccgttgt 33540 tagccgtgcg aaagtttctg gtgagcagat taaccaactg ttaattgaca atggcgtcaa 33600 catcaaagac atgccggacg agaagaaact cgaactgtac aacaagttgg cctaatgctc 33660 acactacctg atttgattgc tcgctatccg gcgttctcaa cactgacaca agctcggttt 33720 gatatcttcc agagcgatgc cgttcttatc atgggtgatg ttgaatcaag atggctgggt 33780 tggtacaatc ctgctatggc agcacttatt gggcatttgc tcactctgaa tgctgcctca 33840 gaagttgagg gtgacgctgc tctcactgct gcacctgtgg ttcgaactga cgttgatgac 33900 gtccaagttg agtttgctga acgtatttgg gataaagttc cttacgttga agcagatttg 33960 tattcaactt catacggtca gcaatatgtt cgttggcgac gtatggcatt tgctgggcct 34020 cgcgtggcct aagaggtcac atgattaacc agaggttggc gtttaaccga tacacaactg 34080 gcgttcattc gattgaaaga tacatctccg gctactatga ttcgaagaac aactgggttg 34140 gtgacactta tgcaccacct gtgaagtttc gctgcacccc aattgggtac ggcgaccgtg 34200 actctggtac aaatgggcaa caacttaaag caactgaaat tggtgaacgt caacctgcat 34260 ttatgcagat tcattcccgt tcagaaatgc caatgaagtc cattatcact gtatatggtt 34320 tgcggtacaa ggtgacacag ataagcgatt acaccgatgc aggcttcttc cgagttattg 34380 tttcaaaaga gttggagaag tagatggata ttcaagacgt cgaaatggaa gcgatgaagg 34440 agattgtcga cgttgctgtc ggcattcctc tttacacgta taagatgcag gagaatgcac 34500 ctcgcccaca aggtgagtat gctgcaatca aatgcacctc aagttttaac cccggctacg 34560 acgagactcg aattgtcacc attggtgggc aggatatgtt tcgtacgcgg ggtgttcgaa 34620 tactcacatt catgattgta ttcagtcgga atggtcaaga gtatatcgac tacgataata 34680 gcttttatcg tcctgatgtt aatgctgtat tgcgcaaaca tgggttttgc gcaatgggca 34740 aagaagctct gagcctcgcc tctatcgcgt tggaaaccaa ttgggaagtt cggcaggggc 34800 tgaaaatgca attcagtgtt ttgcgagagc aaatatcccc aattggtaca atgtcaaatg 34860 cacatgttgg cggcgtattt atcgacggtg acaaagaagt caccataaag ggaaagtaaa 34920 tgtcaattcc tatttccgat atcatcacag ttaacattgc cgtttctcca aacgcagtgg 34980 ctactgacgg attcggccca ctactgttcc taagcaaaga gtttgtccct gttgctggcg 35040 aatatgttgt tcgttcatat tccagcttgg tggaggttac taacgatttc ccaactggcg 35100 aaatccagaa agccgcaaca gcttggtaca gccagaagcc aactccgaaa acgttccttg 35160 ttggtgcgct gcaaactgtt tctggtgctc cggcaacagc ggcatctgtc actgcaactg 35220 ttccttctgt tctggcagat ttgaaactca tcactgctgg tactttgtcg ttctctttga 35280 acggacagcc cgtcaacttg tctgcaattg acttgagtgc tgccgcatct ttcgatgcaa 35340 ttgtcactca gattaatggg gaatttacgg caaacaacgt cgctgcaaca atgtcgcacg 35400 tttcaggtac gtttaaaatc tccagcactg caaccggcgc atctgcaaca atcactgccg 35460 caaccggaac aggtgcagca aacattggtg ttgcaatgaa gttaacggct tccagcaatc 35520 cgctggttgt ccagggtgct gatgctgcgt cgattacaac cgaccttgca gcaattgcga 35580 atacaagctt caactacttc tatgttgcag tcaacaaaga gttgcgagct aaacaagacc 35640 agaaagctgt cgcgctgtgg tgtgaagcaa atggtaaggt gtttggttgg gcggacagcg 35700 atgttaaaat cctgcaagcc aacactgcga acagttttgc agaagcgaag gctcagaacc 35760 tgcgaaacac tatctgcgtc tttgatgcaa gtgtgaacgg cgacgagtat ccagagattt 35820 caattctggc tcgtgctgca actgtgaact tcaacgtcgc aaactccgtc ttgattctgg 35880 cattcaaaac agggccaggt attcgcactg ctgatttgtc ttctggacaa ttggctgcac 35940 tacgttctta caacggcaac gccttcatta aagttggcga caacacgttg tttatggacg 36000 gcaagatggc tgacggcact tggtttgaca cagtgcaggg tgttgagtgg ctgactcaga 36060 aggttcgcaa caatgtgttc aatctgttct acaccagcac aaccaaaatt ccttggactg 36120 aaactggcgt tgcaatggta aaccagcaag ttactctggc actggaattg gctgttacaa 36180 acggcttgat tgcagcgggc tacgataacg aaggcgtctt ctacccagat ggctataaag 36240 ttctttccac cgacttgtca cttctgcaat cgcagaaagg tgctcgtgtt tgggaaggca 36300 cttcattcat tgcaattggt tccggcgcat tgcagggtgc agtaattacc ggcaacttcg 36360 tacagtaagg aagtgaaatg aagcaatata gcttctataa caccgacctc ttaattgatg 36420 gtgcgcgtgt tgatggattc acggcaggcc aagcagttat tactgcacgt cgtaacgcac 36480 cgcagcatgt tcctgtgctg gatgcgtatg gtaagttggc ggttgcaaca actgctgacc 36540 tgtctggtac gattacgttc ccacttctgc aaaccgcaga ttggaatgaa attctgtaca 36600 ccaaagccca actgactcag gcaactggtc tgtcaggcaa caaaaccatg tggcaaccaa 36660 tgcagattca gcttgtcgat aagatgggtg atgttctggt caatggtgtc aatggtgcaa 36720 tcctgcaaca gcctgcaatc attcgtggtg ttacattcac ttcgaatatg tgggctatct 36780 tcgttgaacg tctccagatt aagactggtt cttatccgga tatcggcgtt taatcacaga 36840 gaaagcaaat ggcctgtgaa ttaattcagc gggagtttaa aaactccaat ggcgaccctg 36900 tacttgttgc agttcgtcaa cttccagcgt caaaagcgtt ggagcttcac gttgagttag 36960 ttaatgttgt ggggaactcc gcgttccccc ttattgctaa cgactacgat ttcggcaaca 37020 ttctttcatt aatgcgcgca gctgacccaa aagttatttc cgagctgatg aagcgtgttg 37080 tgtgcatggc tgtcgttgaa ggcatggaag ttaaaccagc cctattcgac atgaaatata 37140 acaacgaact catgcttgtg tgtgaagtgt ttgcatttgt ttgcgaagcc aacttccatg 37200 gggaagcat ccaagcaggc cgagcagaag agttcgagtc cgaagattta agttccctct ttccagaggt 37320 gaactacttt ctgcaccgtc cagtggtgga agatagttca ctttgcactt tgaaagaatt 37380 gcaagatgga acctactccc tcatggactt gttgactttc catgccattc tagatttacg 37440 aatcaaactc aaaccagaag aagaaaatgg caaatagctt aaagcagttg atgagtttat 37500 cagaaccaga gttgattggt ttaggtgctc aatatggtct atccttccac gctggtatga 37560 agaagtcgca gatggctcaa caattgagtc aaagtgctgc gtctggttgg atggaagtta 37620 acaaagaact catgtcagaa agccctgatg gaacatacga cgaaatggtc gggtttcacg 37680 gcacttctga tatgctgtct gatgctgcac acgcagctca aaatattcat gccgctggaa 37740 tgggtggagc atttcaacat tatatgagta gtggtgctgg ttggcaggta gaagctattg 37800 atgactacct caagcaactc ggtgccactg cacaggatgt ttggatgcac ttgccgaaag 37860 agcaagaaga ttctcaccca gcggcattcc gagctttgag caactacatg cgggatactt 37920 tagttggtca ccaagacatc atgcctgaga ttaaagggca tcattctggt gacatcctta 37980 aagagtattc tacaaagctt ggaaacattc aggattcata tgagcatctg tctcacatgt 38040 atcttgacaa atctttgtac gaaaacgggt ctcaatatgc gcatgacatt tcaagagttt 38100 ctgcacgctt ggcaactcaa atgggaagcc gttacttgga agttgctgca agctctgcta 38160 tgggtgctca tgtatcgtat atgtctacgt tacctgctgt tggcgacccg tctgttgttg 38220 ggaatgtcgc tcatgctcgt cagcctctta acgctgctgg acttcctcta ggttcaactg 38280 gttctgctgt ggctggtcgt gagaagtatt ctcttgcatc atctttaaca ggtcttccgg 38340 aaaataatcc tgctgcacaa tctgtttata aaagtattca agaaactgta cataatcttg 38400 ctcaggttta caagggtggg gataatgctt caacaggcat gaatccatac aaacaccgaa 38460 cttctgagta tgacatgatt atggattctg caactcggta caatgatgtt gcagatgttc 38520 gctctggtta tgccaacctt gcaagagaat tggcggacga accaagttat tcagcaacaa 38580 acattcgtgc aaccttggat aacaaccacg acaaaatgga tgcggaatct ttcgctcctg 38640 taggcattgc caatccgagt tttagtcacg cacgtttggc ggaagttgga accagtttca 38700 cagcaaactt ggattcacca acgaattgga atagtgcaag aatgcgcaga gaggaaaaag 38760 caattgctga cctcgatgca tctcatgcaa acttccgtga tgttgcggat ggcggacaaa 38820 gaagttcagg aacaaccata acgtaccatg atgaagttga gcaaggctct ccagaatggc 38880 acgcgatgcg aagcaagtat tcaattactg gctcaaccat tggttcttat cttggcaaca 38940 atgcgtacac cagtccaata aaggaaatgt ccgacaagtt gggcatcaat ccgaagccac 39000 gtgcttccaa ttttgatatg gaacgtggtc accgactgga acctatcgcc cgcgcaaggg 39060 ttggtagaga gtttggattt aatattgggg aatccggtgc aatcacgaac agtgattatc 39120 ctgactttct gtactctcct gacggattaa ttggggatga tgcactttgg gagcacaaag 39180 ctccaagaaa gttcttcgac ttaaaagagc accccgatta caacgaccaa atgcaacttg 39240 gtatgttggt atccaatcgt cagcgaacat tgttttcgca gactgtaggg aatgaaactc 39300 gaagccaatg ggttgagcgt gaccctgact ggtttaacag aaatgaaagc aggataaatt 39360 caactcttgc tcgaatggat gttggacatg aagttggttc tgacaacaat ttcaagacac 39420 cagaagaagc ggagcaagct gcacgtaagg ccatgactgg tgagggcatt tggggattcc 39480 gcacaaagaa ttctggcgaa ggttactaca ctggcggcaa gcgtggaatg tctcggttct 39540 ctgcaactgc gggaactgac gctgatgagt ttgccggacg tcgctctgcg tttgaaccca 39600 acaatgaaga taacggctac cgtccaaact tcgtaatgca agacagcact cagacaggat 39660 tgtcagttat tgggcaaggt tcaggaaatg gtgttgccat ttatgggcaa gctccatccg 39720 aaagcaatcc aaactcatta accaattctg ttaaagaggg tattctcctt gctcaagagg 39780 aaaacagaca aagacgtgga ttccgagaaa tcaacgaaga gtatgcagaa gatgcagact 39840 tcgaagaaga aggtatctcg cgcagtcgtt ttgaccgttc aaatggtggc ggtggccgtc 39900 gtggtggcgg tggtggcggt ggaaggactc caaatccgtg ggatgaacaa tttggtggcc 39960 ccgctggtgg agcggttgct cgcggaattg ctggcggttc tgtaagttct gcaacaaacg 40020 gacttatgac agcaatggcc tcaactcctt ggggacgtgt agctgctgtt gctgctggtg 40080 ctgttcaaat tggtgctgaa cttgctgata atgcaaacga atctttgggc acagctcaag 40140 atgcagggat gacaaatcca gttgcattcg ccagtcaaag gcaaggcatg gaaatgcttg 40200 gtctaagtga aaaccaagca ctaaatgtgg cacaaaccac acattccgca tacaacaccc 40260 tgctgaatgg cgatagcagc ggcgctgtga ggctcgttca gggaacgcgt gggttactaa 40320 ccattagtga tatacgcgag actgagggcg acccagtggc tctggcacgt ataatccaag 40380 agcgtgggcg ggaacgtggg tggagtcagg cgcgggtagc tggcgcaatg gacatggccg 40440 gattgggcgg catggctcgg acgtaccagc gggaaggggc tgtgcaacgc gctgctgagg 40500 ccgtgcggga cgatggcgct gctgctgaaa cagatgatgc ggttaccaat acacaagtgt 40560 tgcgtggcgt ggtgaggcgg gctagccccg aatacggcat tcaaagtgcc tacttccagc 40620 atggtgcaaa ctatgccgca cttgctgcaa ctggtgttca agatgcgttg aatgttggaa 40680 atggtgttgt taatgctggc aattctattg ctgctggtgc tgagaatctc gaaggtcgag 40740 ctaaagagtt tcttgaccac ttcttgccaa atgctgaatc tggaaatcgt gaatatgata 40800 agcatggcaa tcgaatcaca agcccgactg gtgctcgtgg taagtaccag atactgcctt 40860 caacagcacg tgaccctggg tttggtttga ggccatctga tggcactcca caagatgatg 40920 agcgccttgc acgtgaatac atgatgactc tggttgaccg atataatggc gattatgaga 40980 aagccaatgc tgcatacaca gatggtgctg gaacagttga taaggctgtg aagaactacg 41040 gtgatgattg gctcaaacat gtaccacaac aagctcagaa tcgtgttgcg gaatatcgca 41100 agtggagtaa atcaactggt gcaattcagc aaggtgctgg tggctttgct ggttctccaa 41160 tgtccaatcc tgttccgaca gtcattaact taaatgtcaa tgctcaggta aacaacaagc 41220 aggcaactgc aactgttgtg gcaacaaatg ggcaaaccac aactcagact gtaaacatgg 41280 gaagtggtgc tcaacaaaga cgttaaggtt tggcgtcagt cttcgggctg gcgctctact 41340 taagcattag tagaaagtgt aattatttat tgaggttccg ccatgccgtg gatgcgaaga 41400 gttgaagtca tcatcactaa caaggatgat gccagtaaga agacagtatt cagcaaacac 41460 aagattgact ttgaggttcg ctcaacagtt ggttggcctg ctgacactgc aaatgttacg 41520 ctttacaatt taagtcttga ggaagttaaa ttcctgcaag accggaagtt tggaaacttc 41580 tacatcgaga ttcgcgttgg atatgaagat acactttctg acggaagttc ttcgaacata 41640 actgttcatg gaagtggtgg acagaagtct ttggagatgg cctctgtatt gactgctgct 41700 gacacagtgt tctctggcgt tataacaaat gccgttggat acaaacgttc accagaacat 41760 gtcactcaat tgtattgcat atccaaagca tatcttggct ccactcaatt caagcagatg 41820 cggaatataa aagcaggctc gactttaggt gaagcaatta agtccatgtg tgaggactat 41880 gggtttggga ctatctcgaa gtttggtgtc aaggatgaag ttcttgctgc caagttgcta 41940 agaggtcgtg tgtttcatga caccttcttg gttgagtttc gcgcactact gggcgaatac 42000 aatttgttgt ttcaaatgac gacaagcgag attcaaatat tccccgacac ctacggcgat 42060 gt; gttatcggaa atccaattgc tggcatttgc acatatcgtt taaacacatt cataaatgcg 42180 tctatacagc caggcatgat tattgatgtt actccgctgc ttggaaaaga gattctggca 42240 aatggtgtca cagccataca aggtgctggt gttgttttaa acactaacca agctgtcttc 42300 cagtgggcaa tggaggataa atattttatc atggaagttg ttcactttgg ttcgacccac 42360 acaacggaat ttcaaacatc catatctgcc attattggcg ggaacagtgc aatggggttg 42420 aaagaaatgg aatggcagga catgtacgct cgtgctggaa tgactgaggt agaattctaa 42480 tgtcaatgat tgatacaatc acagattttg ctggcaatgt tggtgggaaa gttgccagta 42540 aatttcatta cgggcaccac ccatctataa tcatgtggtc aaatggtgag gctgttcaga 42600 aagaagagcc gtcgctacta ggtcaaatca caggtggcgt agcaaaagcc cttgaagata 42660 tcacaatgtc cggtggcgtg cctgaaagtt ttagttcgtt taagtttgat gcgatggtta 42720 gtgagggaca cgctgcaaca tctcaggtta caagtttccc tgtcagttct ggttttgttg 42780 tttctgacca cgtgatattg caaaaccgaa ttcttaagct gaatgcggtt gcatcaaata 42840 tgcaaaactc tgcaatgtgg gctgcgtcca ttcaaggcct gtctgttatc actggcgcga 42900 tattcaatag cccaatcatt ccgatagtgg gtggtattgc tggtgctgtt gcgtctgcat 42960 ttgaaacaga ggacagaata acatccacat acaacctatt ccgaaacttc caagcaacag 43020 gcaagaagct gtacgtctcc accattcttg gcccgtattt gaattgtatt gtcaccaaac 43080 ttgagtgcaa gcatgacaaa gacacaagcg caattctcag tatcgaaata acactggaag 43140 agttgcaggt gattggcaaa gatgaatttg ccgagcaggc caagaaagcg atggagtcga 43200 tgtatgacta ctctgagttt gcgaagattg caacttctct cggtgttggt gtgttgggtg 43260 gtgtttcatt gcctggcctt ggaagtgcaa aagcaactcc aacagaacaa atgaaagagt 43320 tgcaggctaa gctggatgca cttaaaactc cagcaagttc tgttaagggt gtgatatgag 43380 tgttgaatcc gaagcacaag cctttaaaga attaatgagt aagttgccga cggggtatgt 43440 gaagtttatt cctttctctg cccgcaatgc agtatcgttt gagtttaatg gtgttactgt 43500 tttattatct gctctgaggt ataatgaata ctcaaactgt tatatgtttg atatgtcttg 43560 gagttctact gatatcatct acggtattcc tatccgtgct ggcatcgaca tattgaagca 43620 gtataaaact ccactgccaa acatgtatgc gtcaaaccga acattcccca gccaagaagt 43680 tttaagttgg cgggaaatga atttgttttt aatcgacgtg agtgtcttgg aaaatgtcca 43740 gtcataataa taatcaaaat gctcccgaca ttaacacgag ttatccgggg catatttata 43800 acttcgatgc tgaatctcag acatgtgaag ttcagcttgc gattgaaagt ttgtttattg 43860 ggcatgagga agcttattcc agatatacga aacaaagatt gcagcacgta cctgtgcagt 43920 tcactcaggg tggtggctgg agttttaccc atcccatccc tgatggaact ccttgcaatg 43980 ttcaattcgc gcaacgtggt attgaccact ggctgtttga aaacaaggat gaagcaggaa 44040 ccattgacgg gaaacccgca ccagcattca gtcagttgta ttcacacaat gcagcaacat 44100 gtacagttgg gacgcagcca attccaaaag caatacccga ctttgttggt gacgttatgg 44160 aaatgcgcaa tgaggcaaga agccaaagag tgacattcca cggcaatggt cagattgaac 44220 ttgtcgctgg tagtgccaaa atcactatca attcgtcagg ggaaatctca attgaatcgc 44280 cctctaagat tactgcgaca gcgccgaaaa tcgttttgga tggtgcaaca acaatcacca 44340 aaacactcag cgtgcaaggt ggaggtgctg gtggtggtgc agccgttaca atggttggga 44400 acatcgagca caccggaagc acaaaccaaa ctggctcgtt aacaatcaat ggtgttaaag 44460 ttgatggaca caatcacatc agcaatagcc caggccaaga cacggggcca atgaagaatg 44520 gctaacctca aacttgattc aaaccatgac attattattg gtcgtgggac aacaagagtg 44580 tctggtgcag agcaggttgc gcaactttgc aagtgtcgtt tgctggcact tcttggagaa 44640 tggaagcttg accctaccgt tggtattcct tggtatgatg cgatattctc caaacaagtt 44700 cgcccatctg acattcaagc gacagttgcg aacatcatca gaagcacaaa tggcgttcag 44760 caactgattc aaataagcat tgacgcggat taccgcgaac gtgagttaaa tatttcattt 44820 acggctatct ctgattatgg agatatctcg gagtacctga cgtggcgaca gtacaatacg 44880 gtgtaaccga aaacgggttt gttgcaaaac ctgtgtcagc aatactcgca agcctgaata 44940 caagatttca agctgcattt ggtacaactt ttgatgtaag tccagaatca cctgatggcc 45000 aaactatcgg tattgttgct gatgacattg ccgcatgttg ggcacaagca gaacaagtgt 45060 ttaatgcata tcggccaggc tctgtacaag gtgttggtct tgataacatt tgtgagctta 45120 caaatgctca acgatatgta gaccggcact cacgagcaac tgtggaattg actggagcct 45180 ctggctcgtt agttcctgct ggctcgattg tttctgacgg gaatcgtgag tttgtaaccc 45240 aaactgcgac aacaattccc ggcgatgtta cggttgttgc aagtttgagt ggtgagtatt 45300 acattgcccc aaacacaata aataacatag tttcgaccgg aatctctggc tggactggtg 45360 ttaacaatcc agatgttggt gagacaggaa taacatatga gagcgacgtt gcattgcgtt 45420 caagacgaga caggacgact gcgattgcag gttcagccac agtcgaagca atctatgcgg 45480 cactatctgg cctcaatctt tcttacatcc gtgttcgtga taatgatacc ggcagcccta 45540 ttggtgctca gccatcgggt actatctttg tggtagttga tggcggaaca aagaatgaca 45600 ttgcttctcg catttatgct gctaaaactg gtggtgttcc tacttttggg acggaggata 45660 tcacaattac tgacagcaaa ggatatccgc atcaggtgaa gttcagccgt tcatctccgc 45720 aagaggttta tgtaactggt acattcaaac gacgtgcaaa ctccaacatc agttcaaacg 45780 atgctgcgat tgcattgcag ggtgcgctgg ttacttatct caataattta agtcccggtg 45840 attctgttat ttggtctcat ttgtttgcac cactgagtac ggcgacaacc ggcattgagg 45900 ttgtttcttt gttcataggc attgctgcaa atcctacaca cactgattca attgctcttg 45960 atattgataa gcgtgcgcat ggtactgctg cgaatattgt ctttactgat gtaacaaaca 46020 caccataggt ggaagatggc tggcaagagc ggtttagata tgctcctgtc tgaatatgca 46080 aactccccaa acttgaagaa gtatattaag tgcttccttg atgagattgc agaagtaagg 46140 caggcactcg atgcatcaat ccgttatcgc tatctggcag attcttatgg cgtaatggtt 46200 gatgacattg cctatattgt tggggcatct cgtgtaataa cgggtgccac agcacttggt 46260 tattttgggt tctataaaaa cccacaagca ttcccagctg gcgatgataa caatcccagt 46320 gttggtggtg ttttaaagtc agataatgat aaagactctg gtgactttgt tcgcactgat 46380 atgcagttga agaacgcaat ccgtgcaagg gttttaaaga ttgttggtgc ttgtaccatt 46440 gaacaaacaa tcatgtatct cgacttaatg attgggcgaa gtttaaagac agagattgtt 46500 gagggaagcc tcaagatgga gataaaaatc catgagactt tgtctgttgc agataagctc 46560 atcgtgcat atatgattcc agagttcaag ccttgtggtg ttcaggtgac tttggaagat 46620 gccgcaggac aaatccagtt ggtttactca tcaacatatt atccaccgga gtcactcgta 46680 tgacaaacag attaatgttt cctgaaactt gggcatctgg tggaaccaat gtggctgacc 46740 ctgatttgga tacaaccaat cccagctacg ttgccaacaa gtatgaaggt cagggatgga 46800 tttctgagaa gccaccggaa gaatggcaaa acttcctatc ccagttgact gatgaaaagt 46860 tggtagagtt aatcataaat atggtttact accaagatgc cagtgtgaca tataagccaa 46920 aggccgtagt caagaatggt tcgaatgtat tggcacttgt gtctggtggg acaacaaagc 46980 ccatcctaga tacaacggca gatggataca caaactatgt aaccatgcta gaccagcaat 47040 tgattgggca caaaaacata ccacatgcgc acaacgaaac tgttgattca attatcggcg 47100 ggacttattt aaaaagttac gtggatgagg tttttggctc tccaacgagc ccaagaacaa 47160 ttgtttatca caagttgaaa acaggccgtg cgcatggaga aactccagct caagtcggta 47220 ctcttccggt tggtggtggt atttttacag gggatgtttc attcctgtcg aagatactat 47280 ttgcaggtgc aaccaagtca ttgggattcg tcgcatcgca aggttttgtt gaacttcgct 47340 ctggaagcaa tgccattgct cttgatggtg caggtaatgt ataccacagc acttcatcag 47400 gaagctacaa gatacttcgt gaatcttctt ggcatgagtt tgagacaaag aataatccag 47460 attttgcact gcctgtggag cttatgtctg tctacgttga gactgaactt agcaatcaag 47520 ctggcgttgg tgtctggact ttggagacga gtaatagtcc ctccttcatc gctggacgtg 47580 gtttgtctct gtctggaaac acgataacga taaccaagca atcctatgat ttgcctgtat 47640 ccatctatgt tcatggtgtg gatggactta attctgttgt ttctgttgtg aaggactatc 47700 caaattggca aggcacgaca caaactgtgt ccaacctact gcaatctttg ggtgtcccac 47760 aaatgacgca ccttaagtat ttgaaggtgt atcctcaact gtccgataaa cagaaaacaa 47820 tgttggtgct ataatggcta tacgcccaaa attaaacaga gtctggacaa gttcgagcgc 47880 aactcttcga cgtgacccag gcgatgcaaa atatctacaa ggttggattt cagaaatccc 47940 taccttccaa gttctaaact acttgcagtg gaaagttgac accacaattt tagcaattgc 48000 tgaacgtggt gctgcggagt ggggaactga cgtccaatat gtaaaaggct caattgcttg 48060 ggatgagttg gatggggcaa tttacgttgc gactgttgct gcaccaagca gaacactccc 48120 accatctcag aacagtgcac actggacaag aagctcaatc cagatttctc gtgaagagta 48180 tgtaaatatt gctgctgcta ttgctgcaca tattgcagac accacaggaa atccacatca 48240 cctaactgct gaccgtcttg gtgcatataa caaagctgag attgatgcgc tggttacgac 48300 atatcataat cttgttctgt ctcatgccaa tgacacaaac aatccgcacc agttaactgc 48360 cctcttgatt ggtgctgtac caacaactgg tggaacgtat acaggtgatg tgcagttcaa 48420 cacaaacgtt ttctttgatg cagggaagac aaccaagata tccaatggga gtgggttgtt 48480 cttgcagaat gggaacaatt tggttggtat cgattcctcc ggcaatgctg ttgcaggaga 48540 caccacaacc acatcaaagc ttgttgacga actatcattt ccagttttaa aggcaacaaa 48600 tgaagctgac tatgcaactc catcaccaac gttccagatg cctctggcgt ctgacataaa 48660 catcgtactg ggttctggct tagttgacag tggggcttgg gaaccaaact tctcaatgct 48720 tgacagattg actgcaacaa acgggcagaa cacatatggc tttggttcag ccaacaatgt 48780 tgcagacccg acaaagccaa cgacaatcgc tgttgatgta atgtctagca ctcaacgtgt 48840 cccagcagac acagctgctt ctgttcgcat tgggattacc tcaattggca tctcatttag 48900 cggcggcagt gtaatcagtg ctgagactgc tgggcaaact tcaccaacac atcaggtaac 48960 agggccaatc aatacttggt atcgtgttgt ttgtgtgtgg gaaggtacgt ttgtccgcat 49020 ctatctcaac ggtgtttatg tatccggtcg ggcaattgct gcacaaacag caacagacgg 49080 caagttgcag ttctcaattc ttgggagaca gcctgccgat gctcgtgtgt ggggattccg 49140 gaatcttcga atctggaatg aagcactgtc tgccaaacaa gtttcaacac tttaaggaac 49200 aatgatggcc aaaggttggt cttttagtgc attaataaat gccctcagag gtgccgcatt 49260 cttggatgtt gggacttctg ctggcactgt tgctgcggga gatgattccc gtattgtgaa 49320 tgcggtacag cgaactggtg acaccatggg cggctctctt gccgttcgtg taaaccaagc 49380 tgcgattgca ctccgtcctg tttcagatgg acagacgaat taccttgtcg gcaagaatgc 49440 tgcaaacaat ttggatgttt ggtttgctgg acttcgtgat tccgactctg ttaagtttgg 49500 acgtcaggga tatggcgaac tatatttcaa ggatgatgga acatctggtt gggtcaatcc 49560 tgtttctgga caatctctgt tattcaatga cggcggcgat attttctatc ggtctgcatc 49620 caacggtgtt cactacttca actcagatgt tgaaatccaa agcagtggaa tctccctgaa 49680 aattgcacct gatgcagaca acgaaccgag ttacattgca ggttccaatg cagccaaaac 49740 aggtacaact tggcgagtcg gtaagattgg tgttggtgct gatattgttg ttgcgaactt 49800 ggtttctgga caggcaattg gcttgtcctc gaatggcaac atgtattacg atgttgcaaa 49860 caacaacttc caacacatct tcggccaaac cgttattgcc cctgcaatgc aggttgggaa 49920 cttgactgct accaaagctg gtggcggaga agtcataaca gttaaaacca acacagcatc 49980 cactggcgct tggattagtt tgagaaacag tgccaatgct gagaaaggtc agttgggcat 50040 cctgccagat gggactacga ttgaatttaa gaatgtcgga gttccagcat cagttggttt 50100 tgtcagaatc cttagtgacg gtcaggtgaa tatcggcagc gatgttgatg taaaccttgt 50160 cgtatctccg acaggtgctg ttggtgcaag agggatgtta actgctggtt ctgcacgata 50220 tggcacagat ggaaacattg ttggtactgt ttggggaacc aacgccaaag cgtacgttga 50280 cagtgggtta gccaccaagt tgggtgtgag cgcaaacgca gtcagtgcga ccaagttgca 50340 aacgccaaga aacatcaatg gtgtgccgtt cgatggaact gctgacattc agttggataa 50400 gggtttggtt gttcatgcgt ggggttctta tgccttgttt ggtaatcaga acatcatctt 50460 gacagccatt gatgcaaatc acctaacatt cacaatgact tgggaagcat caaacaactt 50520 tgtgcaactg aataaaacta aagtctcatt ggtttctgtt gacaatgaca cagaagtaat 50580 tcccggctcc acaacaacaa gacgcagaaa gaagattggc gactacacca taaccggatt 50640 gaccttgaca ggttcgattg gtgctaatgc tgtgtttaca atcctctcaa caaaccacgg 50700 cgtaaccgtt ggtcaatcag tgacacttgg actgtgtgcg ttcatttacg accaagttgg 50760 gatgactgtt ggtggacaag ttgcggcaat ccagtactac ggttcagcaa ttcgtttgga 50820 actggacaca ccagtatcaa gtcagtctgt cccaatcttc accagacacc ttcaaggtgc 50880 caactcaaat ggtggttggg ctgcaacaac tgagaagata tatggaagtc agaatgttcc 50940 aggcgaggga accaccacacg ttgcttctgg ctttgtattt gctgatttga aaactgtggg 51000 actaacttcc gcagagacat caagcaacac ttggtatcct gcatcactca tcagtattgt 51060 aattcacaat atagtttgat ggaaaacaaa atggacaggc aaatgcttca atctctaatt 51120 gacctcatgc aacaacatgg cgtctttcat ggattgcttg ctggacttac tgccctaatt 51180 cgtggggcat atgaatcaga agggcttgca aaagctcttc tcgatgctgc actgtgtacg 51240 gtaattggaa cattcgtgtt tgcatttccg ggatttgggg acttgttcaa agagagtcca 51300 caacatgcct tgattgctgc aattgttgtt ggtgtgattg gtgctaacct catcatcaca 51360 actctccgcg attgtttcgc tgctgcactg aaacaactca atcctgcaac ttggtttaag 51420 aaggccaaat aacagaatgg aaagcgccaa tgttaacagc aaaggtgctt gcatttgtct 51480 tggcagggat gttgagcaac aatgctccag cttcggttgg ggcatttcaa caatctgctt 51540 cacaaattgc aagtcaattt tatttagatg gaaatctcaa actatctcat acacaacagc 51600 aagagctaga atgcttggcg aaagttgtct ggtttgaggc gagaggtgaa tcgaaaactg 51660 gtaagactat ggttgctaat gtcgttcgta atcgaacaga atatggaaag ccattcgcaa 51720 ctaccatttg caaagttgta taccaaccta accagtttgc atggacgaga aatagtggaa 51780 agaaaaacac aacattccgt caaattatca aacgacatgg ggaaactgag aaacaagctg 51840 tgctggatac tttggaagtt tcgatgcatg ctgttctgtt tccatcttct cggaaaacaa 51900 ccgccactca cttctgctcg attggggaga aatgtaagtt tcaaagagtg gtcaagttgg 51960 gtagagtcga gagacacaca ttcttcaaat atttaggaaa tgagtaatgg gctggtttat 52020 tcttggttta attctgttaa ttttcttaag ttgatgctga gaaatggtga atttgcgata 52080 gcaaaagcac tattttgagg ctaataaata caaaattccc ttaataataa gtctagtatt 52140 ggcttattct taaagaaatt tactcgttct tggtcggtca actgcaccaa attattccaa 52200 tttcgaacgg aaaaccgatt aagaacactc caaaaacaac aaatatttcc acaaattaac 52260 ggctcatatt gctgcaatat tgctacaata tttacggctc aaagctgttt caatattaac 52320 ggctccaaat attcctatta taaaaaattt ttgggacggc ctacgaatct aattgtgtga 52380 catataaaat ttattttttg aggcgaaagg agggagtgtc cacctcccta tcgggagata 52440 aatacggtcc ctattccaac tttttctcac ctatcggttg agttacaaca gccaaacaag 52500 ttaggtgatg agtcaacgtc aacaccactc gcttcatcat tgtgctccac agttgtatca 52560 gtcgtgggat ggctacgcta actaaatccc tcactcatgc attctgctaa tcatcggcat 52620 tagttgaatg tggatactac aataattgga gcttattatg actatctcaa ttcaaactac 52680 taaatctgtt gttccttcaa tcgctgcact cgtattccgt gctaagcgtg atgtgtttgc 52740 tccacagact tgtgctattg cgtctctcgc aatcttccac atgttgacgg ctctcactcg 52800 tgacgatgtt gtaccagcca tgtcgcttgt tgcacttgca gaagcgtcta tcaagaaggc 52860 taactcatct aacaacgaca tgaagcaata ccactatgtc gacccaacca tcaatctgat 52920 tggattggct aagcactttg gcttcatctc aatgaatgag gacaagtcgt tccaactgac 52980 tgagtcttgg attgagctca tcacaactaa ggattctgca atcccatttg taacaccaat 53040 cacggctgag aatcgtcgca agcctatggt gaaaggtggc aaggtcaaag caagtaagac 53100 tctgaccgct gcaattgatt tcctgcaatc gaccgagtat cacgtcgacc tgcaaatggt 53160 taatgtcatc agcaagatga ttgacatgga ttcacgagtt ggtaagacat ttgcacctgc 53220 aattctgcaa gaacttcacg tctggaatgc tgctaagaat atggctgcac aagatgttct 53280 gttctcagaa tactttgctg acaatcgtgg tcgtttgtat cacgttgcgt gtgctggccc 53340 aaatccacaa tcaagtgact ttgctcgttg cttgtattct cacaatgttg agaacgtggt 53400 caataagctc aatgctgacg gctcaacaac tgccgcatac aacatgttca tggcagagct 53460 cgatgacatt gctggtggca aatggtgtga tgcaaagatg ctcacccgtg tagcacagaa 53520 tccatcaggt gctttgcaaa acttgttgtc tcgtgatgat gcacctaaga agccattcac 53580 atatgttcgt ctggctcttg attggttcca gttcgaaaca acaggtgcat gtgattctcg 53640 tgtaggattt ggtcttgatg ctaaatgttc aggtactcaa tatctggcat tcattgcagg 53700 caatcttgaa atggcacagg caacaggatt ggttgattct gattccaaag ctgctgaccc 53760 atatcaactg tctctggttc aattgatgaa attgctttct cgttcatctt accaaccaga 53820 ccaagcaact ctcgatgctc acctgaacaa gaaaacaggt cgcaacttta tcaagactcc 53880 atacatggct gtgcaatatg gtggtggtac tgctgcactg ttgggcaaca aagatttctg 53940 ccagtatgtc aaacagcagt tgcagattga cgtctctaaa cttgatgact tcgctgaact 54000 ttgtgttgat gcagttcatg cagcacttgg cgagaagatt aacaagttca ttgagcagac 54060 tcagaaagct gttctcgcaa tgtgtgtgaa acttggcaaa gagttcttct catatcgtca 54120 cactgatgga caagttgtac acaagccttg cttcccatct gaggaagtat gtgagccatt 54180 ctcaattcgt gttgactcac aaactcgtgt catctttgga caacagaatg aaggcaaagc 54240 atggtctatt cgttcagcaa atccaactaa agaggaattt gttcgtacct ttgttgttaa 54300 ctacatccaa ggtatcgatg ctttggtggc tcgtgttgtt gctaaacatg ctaagaaagc 54360 tggacttcgt gggtttacat caatccatga ctgcttccgt tgctgcttgg ctgatgcacc 54420 acagatgatg gatgtaatca gagctgccta cattgaggtg ttcgttgata acaatcaatt 54480 cgagcatttg gctaagcaac ttggtggcat caacatgtac catgagaaca tcgtaacgaa 54540 agagttgttg atgagcgaac atgcttatta cttctgccag tagttaaaat acgggaaacg 54600 aagtttcacg aaagataaga ataattctgt taaaagatta tttattatta gccgtgagac 54660 ttcgtctccc gccatcttta aaccgaatat ttcagcaata ttaccgcatg tagtattgct 54720 aacatatttg gcattagtag gaagtgaagt aacagaacac ggagaagtat tccgcatgtc 54780 atgttgagac tggttgcaaa cagattcagc aggtcaactt gaagtacagg tgataatgat 54840 agcctcattg aactccctga gttagctgct caaagttcca atcaccatgc atcaggtacg 54900 aatatcatcc gaaactgtta ctgcatttag gctacctcat ctccgacaaa caggagacag 54960 cgtagctact cagcatgacc agttgctcca atgttagttc ctattataaa ggcccgctca 55020 caagccggga acattggcgt tggaacaatt ggaaattgct gaggaattgg aagagaagtg 55080 aagaagggaa cgagagtc 55098

Claims (5)

Peck tobak Te Solarium Caro sat borum (Pectobacterium carotovorum) with bacteriophage PM-1 (KACC97021P) having a full-genome sequence consisting of the gene nucleotide sequence of SEQ ID NO: 1, which shows the control performance for the vegetable soft rot disease is due. The present invention relates to a method for controlling plant diseases caused by Pectobacterium carotovorum , which comprises the bacteriophage PM-1 (KACC97021P) having an entire genome sequence consisting of the nucleotide sequence of SEQ ID NO: 1 as an active ingredient. Composition. [3] The composition according to claim 2, wherein the vegetable is Chinese cabbage or lettuce. 3. The composition according to claim 2, wherein the vegetable is Pectobacterium carotovorum . delete A composition for suppressing or killing Pectobacterium carotovorum , which comprises as an active ingredient bacteriophage PM-1 (KACC97021P) having an entire genome sequence consisting of the nucleotide sequence of SEQ ID NO: 1.

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