KR101779262B1

KR101779262B1 - Lytic bacteriophage specific for Pseudomonas genus resistant to antibiotics

Info

Publication number: KR101779262B1
Application number: KR1020150166778A
Authority: KR
Inventors: 용동은; 전종수
Original assignee: 연세대학교 산학협력단
Priority date: 2015-11-26
Filing date: 2015-11-26
Publication date: 2017-09-18
Also published as: KR20170061553A

Abstract

본 발명은 강력한 항균제인 카바페넴(carbapenem)에 대한 내성을 보이는 슈도모나스 균속을 용균시키는 박테리오파지 YMC11/06/C171 PPU BP [KCTC18380P]에 관한 것이다.
본 발명에서 제공하는 신규한 박테리오파지는 강력한 항균제인 카바페넴 내성 슈도모나스 균속에 대하여 특이적 사멸능을 가지므로, 상기한 카바페넴 내성 슈도모나스 균속에 의해 유발되는 질환의 예방 및 치료 목적으로 활용할 수 있다. The present invention relates to bacteriophage YMC11 / 06 / C171 PPU BP [KCTC18380P] which lyses Pseudomonas sp. Showing resistance to carbapenem, a potent antimicrobial agent.
The novel bacteriophage provided in the present invention has a specific killing activity against carbapenem-resistant Pseudomonas sp., A potent antimicrobial agent, and thus can be utilized for the prevention and treatment of diseases caused by carbapenem-resistant Pseudomonas sp.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a bacteriophage that lyses Pseudomonas sp. Having antibiotic resistance.

본 발명은 항균제 중에서도 특히 카바페넴(carbapenem)에 대한 내성을 보이는 슈도모나스 균속을 용균시키는 박테리오파지에 관한 것이다.The present invention relates to a bacteriophage which lyses Pseudomonas sp. Which shows tolerance to carbapenem among antibacterial agents.

슈도모나스 균속(Pseudomonas spp.)은 그람 음성 간균으로 포도당 비발효 세균이다. 이들은 토양, 물, 하수 등을 포함한 자연환경, 사람의 피부, 구강, 호흡기계 점막 등에 상재균으로 분포한다. 이들 세균은 신생아, 비장 절제술 시행 환자, 장기간 스테로이드를 투여한 환자, 항암 치료 중인 암환자, 장기 이식 환자, 집중치료실 환자와 같은 면역력이 저하된 고위험군 환자에서의 내인성 기회감염을 일으키는 중요한 병원성 세균이다 [비특허문헌 1]. Pseudomonas spp. Is a gram-negative bacillus, and is a non-fermenting bacterium of glucose. They are distributed in the natural environment including soil, water, sewage, etc., human skin, oral cavity, and respiratory mucosa. These bacteria are important pathogenic bacteria causing endogenous opportunistic infections in newborn infants, spleen resection patients, long-term steroids, cancer patients undergoing chemotherapy, organ transplant patients, and immunocompromised high-risk patients such as intensive care unit patients [ Non-Patent Document 1].

특히 슈도모나스 푸티다(Pseudomonas putida)는 주요 기회감염 원인균으로, 여러 항생제에 내성을 갖는 다재 내성균이다. 최근 NDM-1-생산 다재 내성(NDM-1-producing multidrug-resistant) P.putida가 발견되어 다재내성 슈도모나스 푸티다의 확산 및 증가에 따른 새로운 전략이 필요하다. Pseudomonas putida ( Pseudomonas putida ) is a major opportunistic infectious organism, and is a resistant organism resistant to various antibiotics. Recently, NDM-1-producing multidrug-resistant P. putida has been found and a new strategy for the spread and increase of versatile Pseudomonas putida is needed.

한편, 카바페넴(carbapenem)은 그 항균 범위가 가장 넓고, ESBL이나 AmpC β-락타마제를 포함한 클래스 A, D 및 D β-락타마제에 안정한 가장 강력한 β-락탐 항균제에 해당한다. 그런데 현재 국내뿐만 아니라 세계적으로 카바페넴(carbapenem) 제제조차 내성을 보이는 다제 내성(multidrug resistant) 슈도모나스 균속에 의한 감염이 증가하고 있어서 임상적으로 커다란 문제가 되고 있다. Carbapenem, on the other hand, has the broadest antimicrobial range and is the most potent β-lactam antibiotic stable to Class A, D and D β-lactamase, including ESBL and AmpC β-lactamase. However, there is an increasing clinical problem because of the increased infection by multidrug resistant Pseudomonas spp. Resistant to carbapenem drugs both in Korea and worldwide.

감염증 환자를 감수성을 보이는 항생제로 적절한 치료를 하는 경우 약 90%의 양호한 예후를 기대할 수 있고, 내성을 보이는 항생제로 치료하는 경우는 약 60% 밖에 반응하지 않았다. 이들 세균에 사용할 수 있는 약제는 콜리스틴(colistin), 티제사이클린(tigecyclin)이 남아 있는 실정이나 약제의 부작용 혹은 고가로 인해 그 사용에 어려움이 많다. 더군다나 새로운 항생제 개발이 이미 한계에 도달하고 있으므로 앞으로도 개선의 여지는 매우 적다고 사료된다. 이로써 판단하여 볼 때 항생제 내성 세균의 증가는 광범위한 침습적 시술, 항암 요법, 장기이식 등으로 면역저하 환자가 필연적으로 발생하는 현대 의학의 근간을 흔드는 심각한 문제라고 판단된다. Approximately 90% of patients with infectious disease susceptibility to antibiotics are expected to have a good prognosis, and only 60% of patients with resistant antibiotics have responded. The drugs that can be used for these bacteria are colistin and tigecycline, but they are difficult to use because of side effects or high price of drugs. Furthermore, since the development of new antibiotics has already reached its limit, there is little room for improvement in the future. Based on these results, it is considered that the increase of antibiotic resistant bacteria is a serious problem that paves the foundation of modern medicine in which immune compromised patients necessarily arise due to extensive invasive procedures, chemotherapy and organ transplantation.

과거 1960년대와 1970년대 초반까지 항생제 개발에 괄목할 만한 많은 성과가 있었고, 이를 통하여 많은 감염증이 치료되었다. 그러나 40 여년이 지나고 있는 요즘 감염병은 전세계적으로 2번째 흔한 사인이자, 미국 내에서는 3번째 사인으로 보고되어서 [비특허문헌 2] 그 심각성이 줄어들지 않고 있다. 더군다나 최근 여러 항생제에 내성인 세균이 출현, 증가하고 있어서 약제 선택의 폭이 현저히 줄어들고 있는 실정이다.Until the 1960s and early 1970s, there have been remarkable achievements in the development of antibiotics, and many infections have been treated. However, in recent decades, infectious diseases have been reported to be the second most common cause worldwide and the third most common cause of death in the United States [Non-Patent Document 2]. Furthermore, the number of antibiotic-resistant bacteria has been increasing and the number of medicines has been greatly reduced.

비록 항생제가 박테리아에 의한 감염성 질환의 치료에 있어 여전히 주된 방법으로 널리 사용되고 있는 실정이지만, 과도한 항생제의 사용으로 인하여 1980년대부터 많은 항생제 내성 균주가 발생하기 시작했으며 상기 예시된 바와 같이 더 이상 기존 항생제로는 효과적 치료를 기대할 수 없는 일명 슈퍼박테리아(super-bacteria) 또는 슈퍼버그(super-bug)라 불리는 내성균(resistant bacteria)까지 발생하여 사회적으로 크나큰 문제로 대두되고 있다. 이런 이유로 기존 항생제에 대하여 내성을 획득한 박테리아에 의한 질환까지도 치료할 수 있는 새로운 항생 물질의 개발이 시급하다 할 수 있다.Although antibiotics are still widely used as a main method in the treatment of infectious diseases caused by bacteria, many antibiotic resistant strains have been started to develop from the 1980s due to excessive use of antibiotics. As shown above, Has emerged as a socially major problem because of the occurrence of resistant bacteria called super-bacteria or super-bugs, which can not be expected to be treated effectively. For this reason, it is urgent to develop new antibiotics that can treat diseases caused by bacteria that have acquired tolerance to existing antibiotics.

이처럼 내성균이 증가하게 된 주된 원인으로는 무분별한 항생제 오남용(mis- and over-use)으로 인한 사람, 가축, 야생동물, 양식어류, 토양, 하천, 바다 등 환경 전체의 항생제 오염을 들 수 있다. 항생제 과다 사용이 새롭게 개발된 항생제에 대한 박테리아의 내성 획득 시간을 과거보다 더 짧게 만드는 하나의 원인이 되고 있는 것이다. 그러나 보다 더 근본적인 원인은 새롭게 개발된 합성 항생제 대부분이 과거 개발된 항생제의 기본 골격 구조를 그대로 유지하면서 화학적으로 약간의 구조만을 변경시키는 방식으로 개발되고 있는 기술적 한계에 크게 기인하고 있다고 할 수 있다. 현재 시장에 나와 있는 모든 항생제는 내성 문제에 직면하고 있으며, 이에 따라 기존 항생제의 내성 문제에서 자유로운 새로운 계열(class)의 항생제의 개발에 대한 요구가 큰 실정이다. 그럼에도 불구하고 지난 50년 동안 발견된 새로운 항생제 계열은 옥사졸리디논(Oxazolidinones), 고리형 리포펩타이드(cyclic lipopeptides), 프라텐시마이신(platensimycin)의 3 가지뿐이다. 따라서 새로운 항생제 계열에 대한 요구는 여전하다고 할 수 있으며, 이들 새로운 항생제는 현재 일반적으로 사용되는 항생제에 대하여 내성이 생긴 박테리아에도 항균 활성이 있어야 할 것이다.The main reason for the increase in the number of resistant bacteria is antibiotic contamination of whole environment such as human, livestock, wild animals, aquaculture, soil, river and sea due to irrelevant antibiotic mis- and over-use. The overuse of antibiotics is one of the reasons to make bacterial resistance acquisition time of newly developed antibiotics shorter than in the past. However, the more fundamental cause is that most newly developed synthetic antibiotics are largely due to technological limitations that are developed in such a way that only the structure of the antibiotics is modified chemically while maintaining the basic skeletal structure of the previously developed antibiotics. All current antibiotics on the market face resistance problems, and there is a great demand for the development of new class of antibiotics free from the problem of resistance to existing antibiotics. Nevertheless, there are only three new classes of antibiotics discovered over the last 50 years: oxazolidinones, cyclic lipopeptides, and platensimycin. Therefore, the demand for new antibiotics is still present, and these new antibiotics should have antimicrobial activity against bacteria that are resistant to commonly used antibiotics.

항생제 오남용 및 이로 인한 내성균 출현 등으로 대표되는 기존 합성 항생제의 문제점이 세계적으로 크게 이슈화되어 있는 상황에서 이에 대한 해결책을 제시해 줄 수 있는 대상으로 주목을 받고 있는 것이 바로 박테리오파지(Bacteriophage) 및 박테리오파지 유래의 리신(Lysin) 단백질이다. 박테리오파지 및 리신 단백질은 기존 항생 물질과는 완전히 다른 계열이라 할 수 있으며 이에 따라 전혀 다른 항균(antibacterial) 작용 기작(mode of action)을 갖고 있다. 따라서 기존 항생제의 사용에서 나타나던 여러 부작용(side effect)이나 문제점을 개선할 수 있을 것으로 기대를 모으고 있다.The problem of existing synthetic antibiotics such as abuse of antibiotics and the emergence of resistant bacteria caused by the antibiotics has been a major issue in the world, and it has been attracting attention as a possible solution for this problem. Bacteriophages and bacteriophage-derived lysine (Lysin) protein. Bacteriophages and lysine proteins are completely different from existing antibiotics and thus have completely different antibacterial mode of action. Therefore, it is anticipated that it will be possible to improve various side effects or problems in the use of existing antibiotics.

박테리오파지는 박테리아를 감염시킬 수 있으며 보통 파지(phage)라고 줄여서 부르기도 한다. 박테리오파지는 핵산으로 이루어진 유전물질 중심부를 단백질 외피가 싸고 있는 단순한 구조의 유기체이며, 핵산은 단일 사슬이거나 이중 사슬인 DNA 또는 RNA로 되어있다. 박테리오파지는 생존에 숙주(host)가 반드시 필요하며, 모든 박테리아에는 특정 박테리오파지가 존재한다고 알려져 있다. 박테리오파지는 박테리아의 세포벽(cell wall)의 펩티도글리칸(peptidoglycan) 층(layer)을 공격하여 세포벽을 파괴함으로써 박테리아를 사멸시킬 수 있다. 박테리오파지는 1915년 영국의 세균학자 Twort와 1917년 프랑스의 dHerelle에 의해 독립적으로 규명되었으며 세균을 잡아먹는다는 뜻에서 박테리오파지라고 명명되었다. 박테리오파지의 항박테리아 활성(antibacterial activity) 때문에 발견 직후부터 인체와 동물의 질병 치료에 사용되었다. 그러나 Flemming에 의해 페니실린이 발견된 이후, 계속된 다양한 항생제의 등장 및 보급화로 인하여 서구에서는 관심이 점차 줄어들게 되었다. 그러나 러시아와 독일 등의 동구권 나라들은 박테리오파지 연구를 계속 해왔으며 관련하여 다수의 제품들이 산업화된 바도 있다. 하지만, 2000년대에 이르러 항생제 내성균 문제가 부각되면서 서구에서도 다시 박테리오파지에 대하여 관심을 갖게 되었으며 그 후 약 7~8년이 흐른 최근, 산업화 사례들이 속속 보고되고 있다. 즉, 박테리오파지가 세계적으로 새롭게 재조명되기 시작한 시기는 2000년대 초반으로, 실질적 산업화 노력이 약 7 ~ 8 여년 정도 진행된 신규한 분야로서, 생명공학기술의 발전과 맞물려 최근 매우 활발히 관련 개발이 진행되고 있는 분야라 할 수 있다.Bacteriophages can infect bacteria and are often called phages. A bacteriophage is a simple structure organism with a protein envelope wrapped around a nucleus of genetic material, and the nucleic acid is a single chain or double-stranded DNA or RNA. A bacteriophage is a host that is required to survive, and all bacteria are known to have certain bacteriophages. Bacteriophages can kill bacteria by attacking the peptidoglycan layer of the cell wall of bacteria and destroying the cell walls. The bacteriophage was independently identified by the British bacteriologist Twort in 1915 and dHerelle in France in 1917 and was named bacteriophage in order to eat bacteria. Because of the antibacterial activity of bacteriophage, it was used for the treatment of diseases of humans and animals immediately after its discovery. However, since the discovery of penicillin by Flemming, interest has gradually diminished in the West due to the emergence and spread of various antibiotics. However, the Eastern bloc countries such as Russia and Germany have continued to study bacteriophage, and many products have been industrialized. However, as the problem of antibiotic-resistant bacteria has emerged in the 2000s, the bacteriophage became interested in the bacteriophage again in the West. Recently, industrialization cases have been reported about 7 ~ 8 years later. In other words, bacteriophage began to be reemerged in the world in the early 2000s, and it is a new field where practical industrialization efforts have been carried out for about 7 to 8 years. In the field of biotechnology, .

박테리오파지의 가장 큰 특징으로는, 감염성 박테리아 속에 침투한 다음 박테리아의 세포벽을 파괴하여 박테리아의 사멸을 유도한다는 것이며, 이러한 박테리오파지에 의한 세포 사멸의 기작은 세균의 세포벽 합성을 방해하는 방식의 기존 합성 항생제의 기작과는 완전히 다르다. 따라서 기존 합성 항생제에 대한 감수성에 관계없이 자신의 항균 활성을 발휘할 수 있다. 이로 인하여 특히 기존 항생제에 대하여 이미 내성을 획득한 내성균에 대해서도 항균 효과가 있다. 또한, 박테리아에 대해서는 매우 특이적인 항균 활성을 가지고 있기 때문에 인간을 포함한 동물을 구성하는 세포인 진핵세포(eukaryotic cell)에는 영향을 주지 않는다는 장점을 갖고 있다.The major feature of bacteriophage is that it penetrates into infectious bacteria and then destroys the cell walls of the bacteria to induce the death of the bacteria. The mechanism of this cell death by bacteriophage is that of existing synthetic antibiotics It is completely different from the mechanism. Therefore, it can exert its antimicrobial activity irrespective of susceptibility to existing synthetic antibiotics. Therefore, it has an antibacterial effect against resistant bacteria that have already acquired tolerance to existing antibiotics. In addition, it has an advantage that it does not affect eukaryotic cells, which constitute animals including humans, because they have a very specific antibacterial activity against bacteria.

Patrick PR, et al. eds. Manual of Clinical Microbiology. 8th eds. ASM press. 2003Patrick PR, et al. eds. Manual of Clinical Microbiology. 8th eds. ASM press. 2003 Pinner RW. Trends in infectious diseases mortality in the United States. JAMA. 1996:275:189Pinner RW. Trends in infectious diseases in the United States. JAMA. 1996: 275: 189

본 발명의 일 목적은 카바페넴 내성 슈도모나스 균속에 대한 특이적 감염 및 사멸능을 갖는 신규한 박테리오파지를 제공하는 것이다.It is an object of the present invention to provide a novel bacteriophage having a specific infectious and killing ability against carbapenem-resistant Pseudomonas spp.

본 발명의 다른 목적은 카바페넴 내성 슈도모나스 균속에 대한 특이적 감염 및 사멸능을 갖는 신규한 박테리오파지에 의해 유발되는 질환의 예방 및 치료용 조성물과 질환 개선용 식품 조성물을 제공하는 것이다.Another object of the present invention is to provide a composition for the prevention and treatment of diseases caused by a novel bacteriophage having a specific infectious and killing activity against carbapenem-resistant Pseudomonas sp. And a food composition for improving disease.

본 발명의 발명자들은 카바페넴 내성 세균 감염증 치료의 대안 물질로 기존의 화학용법 제제를 보완할 수 있는 박테리오파지를 검토하던 중 카바페넴 내성 슈도모나스 푸티다(Pseudomonas putida)를 선택적으로 사멸시킬 수 있는 박테리오파지를 분리하고, 분리된 박테리오파지의 형태적 및 유전적 특성을 분석하여 이를 타 박테리오파지와 구별하여 특정 지을 수 있는 유전체(genome)의 유전자 서열을 제공하며, 더 나아가 카바페넴 내성 슈도모나스 푸티다 특이적 사멸능을 갖는 분리된 박테리오파지를 이용하여 카바페넴 내성 슈도모나스 푸티다에 의해 유발되는 질환의 예방 및 치료 목적으로 이용함으로써 본 발명을 완성하였다.The inventors of the present invention have been studying bacteriophages that can complement existing chemical agents as an alternative to carbapenem resistant bacterium infection, and have found that bacteriophages capable of selectively killing carbapenem-resistant Pseudomonas putida are isolated And analyzes the morphological and genetic characteristics of the isolated bacteriophage to distinguish it from other bacteriophages and provides a gene sequence of a genome that can be identified. Furthermore, it has a carbapenem-resistant pseudomonas putida- The present invention has been completed by using the isolated bacteriophage for the purpose of preventing and treating diseases caused by carbapenem-resistant Pseudomonas putida.

구체적으로, 본 발명자들은 카바페넴 내성 슈도모나스 푸티다를 선택적으로 사멸시키기 위해 예의 노력한 결과, 병원체로부터 슈도모나스 푸티다를 분리하고, 이 슈도모나스 푸티다를 특이적으로 사멸시킬 수 있는 신규한 박테리오파지를 선별하였고 이를 YMC11/06/C171 PPU BP로 명명하였다. 이렇게 선별된 박테리오파지 YMC11/06/C171 PPU BP를 2015년 4월 29일자로 한국생명공학연구원 미생물자원센터 (기탁번호 KCTC18380P)에 기탁하였다.Specifically, the present inventors have made intensive efforts to selectively kill carbapenem-resistant Pseudomonas putida, and as a result, they have succeeded in isolating Pseudomonas putida from a pathogen and selecting a novel bacteriophage capable of specifically killing Pseudomonas putida, / C171 PPU BP. The selected bacteriophage YMC11 / 06 / C171 PPU BP was deposited with the Center for Microbial Resources, Korea Research Institute of Bioscience and Biotechnology (Accession No. KCTC18380P) on April 29, 2015.

본 발명의 일 구현 예에 따르면 항생제 내성 슈도모나스 균속에 대하여 선별된 박테리오파지는 서열번호 2 내지 21 중 적어도 하나로 표시되는 유전체를 포함할 수 있으며, 바람직하게는 서열번호 2, 7 및 15 중 적어도 하나로 표시되는 유전체를 포함할 수 있다. According to one embodiment of the present invention, the bacteriophage selected for the antibiotic-resistant pseudomonas sp. May comprise a dielectric represented by at least one of SEQ ID NOS: 2 to 21, preferably at least one of SEQ ID NOS: 2, 7 and 15 And may include a dielectric.

본 발명에서 상기 박테리오파지의 유전체의 핵산 분자 서열을 제공한다 (서열번호 1).In the present invention, a nucleic acid molecule sequence of the genome of the bacteriophage is provided (SEQ ID NO: 1).

본 발명에서 상기 항생제 내성 슈도모나스 균속에 속하는 균종은 슈도모나스 푸티다 (Pseudomonas putida)일 수 있다. In the present invention, the microorganism belonging to the antibiotic-resistant Pseudomonas sp. Can be Pseudomonas putida.

본 발명에서 상기 항생제는 카바페넴일 수 있고, 상기 항생제 내성 슈도모나스 균속이 생산하는 효소는 카바페넴아제일 수 있다. In the present invention, the antibiotic may be carbapenem, and the enzyme produced by the antibiotic-resistant Pseudomonas sp. May be carbapenemase.

본 명세서에서 사용된 "핵산 분자"라는 용어는 DNA (gDNA 및 cDNA) 그리고 RNA 분자를 포괄적으로 포함하는 의미를 가지며, 핵산 분자에서 기본 구성 단위인 뉴클레오타이드는 자연의 뉴클레오타이드 뿐만 아니라, 당 또는 염기 부위가 변형된 유사체(analogue)도 포함한다(Scheit, Nucleotide Analogs, John Wiley, New York(1980); Uhlman 및 Peyman, Chemical Reviews, 90:543-584(1990)).As used herein, the term "nucleic acid molecule" is intended to encompass both DNA (gDNA and cDNA) and RNA molecules, and the nucleotide, which is a basic constituent unit in the nucleic acid molecule, includes not only natural nucleotides, Also included are modified analogues (Scheit, Nucleotide Analogs, John Wiley, New York (1980); Uhlman and Peyman, Chemical Reviews, 90: 543-584 (1990)).

본 발명의 다른 구현 예에 따르면, 항생제 내성 슈도모나스 균속에 의해 유발되는 질환의 예방 또는 치료용으로서 박테리오파지 YMC11/06/C171 PPU BP [KCTC18380P]를 유효성분으로 포함하는 약학적 조성물을 제공한다.According to another embodiment of the present invention, there is provided a pharmaceutical composition comprising, as an active ingredient, bacteriophage YMC11 / 06 / C171 PPU BP [KCTC18380P] for the prevention or treatment of diseases caused by antibiotic resistant Pseudomonas sp.

본 발명의 약학적 조성물은 1 × 10³ 내지 1 × 10¹⁰ pfu/㎖의 박테리오파지를 포함하며, 바람직하게는 1 × 10⁶ 내지 1 × 10⁹ pfu/㎖의 박테리오파지를 포함할 수 있다. The pharmaceutical composition of the present invention comprises 1 × 10 ³ to 1 × 10 ¹⁰ pfu / ml of bacteriophage, preferably 1 × 10 ⁶ to 1 × 10 ⁹ pfu / ml of bacteriophage.

본 발명의 약학적 조성물에 포함되는 박테리오파지 YMC11/06/C171 PPU BP [KCTC18380P]는 상술한 바와 같이, 항생제 내성 슈도모나스 균속, 바람직하게는 카바페넴 내성 슈도모나스 푸티다를 특이적으로 사멸시키므로, 슈도모나스 푸티다 의해 유발되는 다양한 질환의 치료에 효과를 나타낸다.As described above, the bacteriophage YMC11 / 06 / C171 PPU BP [KCTC18380P] contained in the pharmaceutical composition of the present invention specifically kills antibiotic-resistant pseudomonas spp., Preferably carbapenem-resistant pseudomonas spp. It is effective in the treatment of various diseases caused.

슈도모나스 푸티다는 폐렴, 뇌수막염, 균혈증, 심내막염 및 내인성 기회감염 등을 일으키는 원인균으로서, 본 발명의 약학적 조성물은 슈도모나스 푸티다에 의해 유발되는 다양한 질환(슈도모나스 푸티다 유발성 질환), 예컨대 폐렴, 뇌수막염, 균혈증, 심내막염 및 내인성 기회감염 등에 대한 치료에 이용될 수 있다. Pseudomonas putida is a causative organism causing pneumonia, meningitis, bacteremia, endocarditis and endogenous opportunistic infection. The pharmaceutical composition of the present invention is useful as a medicament for treating various diseases (Pseudomonas putida-induced diseases) caused by Pseudomonas putida such as pneumonia, Bacteremia, endocarditis, endogenous opportunistic infections, and the like.

본 명세서에서 사용된 '치료'라는 용어는 (ⅰ) 슈도모나스 푸티다에 의해 유발된 질환의 예방; (ⅱ) 슈도모나스 푸티다에 의해 유발된 질환의 억제; 및 (ⅲ) 슈도모나스 푸티다에 의해 유발된 질환의 경감을 의미한다.The term " treatment ", as used herein, refers to (i) prevention of a disease caused by Pseudomonas putida; (Ii) inhibition of the disease caused by Pseudomonas putida; And (iii) relieving the disease caused by Pseudomonas putida.

또한, 본 발명에서 상기 약학적 조성물은 통상의 방법에 따른 적절한 담체, 부형제 또는 희석제를 추가로 포함할 수 있다. 본 발명의 약학적 조성물에 포함될 수 있는 담체, 부형제 및 희석제로는 락토즈, 덱스트로즈, 수크로스, 솔비톨, 만니톨, 자일리톨, 에리스리톨, 말티톨, 전분, 아카시아 고무, 알지네이트, 젤라틴, 칼슘 포스페이트, 칼슘 실리케이트, 셀룰로즈, 메틸 셀룰로즈, 미정질 셀룰로스, 폴리비닐 피롤리돈, 메틸히드록시 벤조에이트, 프로필히드록시벤조에이트, 탈크, 마그네슘 스테아레이트 및 광물유 등을 사용할 수 있으나, 이에 제한되지 않는다. In addition, in the present invention, the pharmaceutical composition may further comprise an appropriate carrier, excipient or diluent according to a conventional method. Examples of carriers, excipients and diluents that can be included in the pharmaceutical composition of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium But are not limited to, silicates, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

또한, 본 발명에 따른 약학적 조성물은, 각각 통상의 방법에 따라 산제, 과립제, 정제, 캡슐제, 현탁액, 에멀젼, 시럽, 에어로졸 등의 경구형 제형, 외용제, 좌제 또는 멸균 주사용액의 형태로 제형화하여 사용될 수 있다. 상세하게는, 제제화할 경우에는 보통 사용하는 충진제, 증량제, 결합제, 습윤제, 붕해제, 계면활성제 등의 희석제 또는 부형제를 사용하여 조제될 수 있다. 경구 투여를 위한 고형제제에는 정제, 환제, 산제, 과립제, 캡슐제 등이 포함되며, 이러한 고형제제는 상기 본 발명의 약학적 조성물에 적어도 하나 이상의 부형제 예를 들면, 전분, 칼슘카보네이트 (calcium carbonate), 수크로스 (sucrose), 락토오스 (lactose), 젤라틴 등을 섞어 조제될 수 있다. 또한, 단순한 부형제 이외에 마그네슘 스테아레이트, 탈크 같은 윤활제들도 사용될 수 있다. 경구를 위한 액상 제제로는 현탁제, 내용액제, 유제, 시럽제 등이 해당되는 데, 흔히 사용되는 단순 희석제인 물, 리퀴드 파라핀 이외에 여러 가지 부형제, 예를 들면 습윤제, 감미제, 방향제, 보존제 등이 포함될 수 있다. 비경구 투여를 위한 제제에는 멸균된 수용액, 비수성용제, 현탁제, 유제, 동결건조 제제 및 좌제가 포함된다. 비수성용제, 현탁제로는 프로필렌글리콜 (propylene glycol), 폴리에틸렌 글리콜, 올리브 오일과 같은 식물성 기름, 에틸올레이트와 같은 주사 가능한 에스테르 등이 사용될 수 있다. 좌제의 기제로는 위텝솔 (witepsol), 마크로골, 트윈 (tween) 61, 카카오지, 라우린지, 글리세로젤라틴 등이 사용될 수 있다. The pharmaceutical composition according to the present invention may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, external preparations, suppositories or sterilized injection solutions, Can be used. More specifically, when formulating the composition, it can be prepared using a diluent or an excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, a surfactant, and the like. The solid preparations for oral administration include tablets, pills, powders, granules, capsules and the like. These solid preparations can be prepared by mixing at least one excipient such as starch, calcium carbonate, Sucrose, lactose, gelatin, and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, syrups and the like, and various excipients such as wetting agents, sweeteners, fragrances, preservatives, etc. in addition to commonly used diluents such as water and liquid paraffin . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of the suppository base include witepsol, macrogol, tween 61, cacao paper, laurin, glycerogelatin and the like.

본 발명에 따른 약학적 조성물의 투여 경로는 이들로 한정되는 것은 아니지만 구강, 정맥내, 근육내, 동맥내, 골수내, 경막내, 심장내, 경피, 피하, 복강내, 비강내, 장관, 국소, 설하 또는 직장이 포함된다. 경구 또는 비경구 투하가 바람직하다. 본 발명에서 사용된 용어 "비경구"는 피하, 피내, 정맥내, 근육내, 관절내, 활액낭내, 흉골내, 경막내, 병소내 및 두개골내 주사 또는 주입기술을 포함한다. 본 발명의 약학적 조성물은 또한 직장 투여를 위한 좌제의 형태로 투여될 수 있다.The route of administration of the pharmaceutical composition according to the present invention may be, but is not limited to, oral, intravenous, intramuscular, intraarterial, intramedullary, intrathecal, intracardiac, transdermal, subcutaneous, intraperitoneal, intranasal, , Sublingual or rectal. Oral or parenteral administration is preferred. The term "parenteral" as used herein includes subcutaneous, intradermal, intravenous, intramuscular, intraarticular, intrasynovial, intrasternal, intrathecal, intralesional and intracranial injection or infusion techniques. The pharmaceutical compositions of the present invention may also be administered in the form of suppositories for rectal administration.

본 발명의 약학적 조성물은 사용된 특정 화합물의 활성, 연령, 체중, 일반적인 건강, 성별, 정식, 투여시간, 투여경로, 배출율, 약물 배합 및 예방 또는 치료될 특정 질환의 중증을 포함한 여러 요인에 따라 다양하게 변할 수 있고, 상기 약학적 조성물의 투여량은 환자의 상태, 체중, 질병의 정도, 약물 형태, 투여경로 및 기간에 따라 다르지만 당업자에 의해 적절하게 선택될 수 있고, 1일 0.0001 내지 50mg/kg 또는 0.001 내지 50mg/kg으로 투여할 수 있다. 투여는 하루에 한번 투여할 수도 있고, 수회 나누어 투여할 수도 있다. 상기 투여량은 어떠한 면으로든 본 발명의 범위를 한정하는 것은 아니다. 본 발명에 따른 약학적 조성물은 환제, 당의정, 캡슐, 액제, 겔, 시럽, 슬러리, 현탁제로 제형될 수 있다.The pharmaceutical composition of the present invention may be administered orally or parenterally depending on various factors including the activity of the specific compound used, age, weight, general health, sex, diet, time of administration, route of administration, rate of excretion, drug combination, And the dosage of the pharmaceutical composition may be appropriately selected by a person skilled in the art depending on the condition of the patient, the body weight, the degree of disease, the type of drug, the route of administration and the period of time, and is preferably from 0.0001 to 50 mg / kg or 0.001 to 50 mg / kg. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way. The pharmaceutical compositions according to the present invention can be formulated into pills, dragees, capsules, solutions, gels, syrups, slurries, suspensions.

본 발명의 또 다른 구현 예에 따르면, 본 발명은 박테리오파지 YMC11/06/C171 PPU BP [KCTC18380P]를 유효성분으로 포함하는 항생제를 제공한다.According to another embodiment of the present invention, the present invention provides an antibiotic comprising the bacteriophage YMC11 / 06 / C171 PPU BP [KCTC18380P] as an active ingredient.

대부분의 항생 물질들은 내성 증가에 직면함에 따라 갈수록 사용범위가 줄어들 수밖에 없는데 반해, 본 발명의 박테리오파지 YMC11/06/C171 PPU BP [KCTC18380P]를 유효성분으로 포함하는 항생제는 내성 문제를 근본적으로 해결할 수 있기에 그 만큼 항생제로서의 제품수명이 길어질 것으로 기대된다. 따라서, 병원성 세균인 슈도모나스 푸티다를 특이적으로 사멸시키는 본 발명의 박테리오파지 YMC11/06/C171 PPU BP [KCTC18380P]를 유효성분으로 포함하는 항생제는 항균 효과, 살균 효과 및 방부 효과가 뛰어난 항생제로 유용하게 사용될 수 있다. 본 명세서에서 사용된 '항생제'라는 용어는 방부제, 살균제 및 항균제를 총칭한다.Antibiotics containing the bacteriophage YMC11 / 06 / C171 PPU BP [KCTC18380P] of the present invention as an active ingredient can fundamentally solve the resistance problem, while the antibiotics of most of the antibiotics are subject to an increase in resistance as the resistance increases. It is anticipated that the product life as antibiotics will be longer. Therefore, antibiotics containing the bacteriophage YMC11 / 06 / C171 PPU BP [KCTC18380P] of the present invention which specifically kill Pseudomonas putida, which is a pathogenic bacterium, as an active ingredient are useful as antibiotics excellent in antibacterial effect, sterilization effect and preservative effect . The term " antibiotic " as used herein collectively refers to preservatives, bactericides and antibacterial agents.

또한, 본 발명의 또 다른 구현 예에 따르면, 본 발명은 상기 박테리오파지 YMC11/06/C171 PPU BP [KCTC18380P]를 유효성분으로 포함하는 소독제를 제공한다.Further, according to another embodiment of the present invention, the present invention provides a disinfectant comprising the bacteriophage YMC11 / 06 / C171 PPU BP [KCTC18380P] as an active ingredient.

카바페넴 내성 슈도모나스 푸티다에 대하여 특이적 사멸능을 갖는 본 발명의 박테리오파지 YMC11/06/C171 PPU BP [KCTC18380P]를 유효성분으로 포함하는 소독제는 병원 감염을 막기 위한 병원 및 보건용의 소독제로 유용하게 사용될 수 있고, 또한 일반 생활 소독제, 식품 및 조리 장소 및 설비의 소독제, 축산산업의 축사 소독제로서 유용하게 사용될 수 있다.The disinfectant containing the bacteriophage YMC11 / 06 / C171 PPU BP [KCTC18380P] of the present invention having the specific killing ability against carbapenem-resistant Pseudomonas putida as an active ingredient is useful as a disinfectant for hospital and public health to prevent hospital infection It can also be used as a general disinfectant, a disinfectant for food and cooking places and facilities, and as a barn disinfectant for the livestock industry.

본 발명의 또 다른 구현 예에 따르면, 항생제 내성 슈도모나스 균속에 의해 유발되는 질환의 개선용으로서 박테리오파지 YMC11/06/C171 PPU BP [KCTC18380P]를 유효성분으로 포함하는 식품 조성물을 제공한다.According to another embodiment of the present invention, there is provided a food composition comprising bacteriophage YMC11 / 06 / C171 PPU BP [KCTC18380P] as an active ingredient for the improvement of diseases caused by antibiotic resistant Pseudomonas sp.

본 발명의 식품 조성물은 1 × 10³ 내지 1 × 10¹⁰ pfu/㎖의 박테리오파지를 포함하며, 바람직하게는 1 × 10⁶ 내지 1 × 10⁹ pfu/㎖의 박테리오파지를 포함할 수 있다. The food composition of the present invention contains 1 × 10 ³ to 1 × 10 ¹⁰ pfu / ml of bacteriophage, preferably 1 × 10 ⁶ to 1 × 10 ⁹ pfu / ml of bacteriophage.

본 발명의 식품 조성물에 포함되는 박테리오파지 YMC11/06/C171 PPU BP [KCTC18380P]는 상술한 바와 같이, 항생제 내성 슈도모나스 균속, 바람직하게는 카바페넴 내성 슈도모나스 푸티다를 특이적으로 사멸시키므로, 슈도모나스 푸티다에 의해 유발되는 다양한 질환에 개선 효과를 나타낸다.Since bacteriophage YMC11 / 06 / C171 PPU BP [KCTC18380P] contained in the food composition of the present invention specifically kills antibiotic-resistant Pseudomonas spp., Preferably carbapenem-resistant Pseudomonas spp., As described above, Pseudomonas putida And exhibits an improvement effect on various diseases caused.

본 명세서에서 사용된 '개선'이라는 용어는 슈도모나스 푸티다에 의해 유발된 질환의 경감을 의미한다.As used herein, the term " improvement " refers to alleviation of the disease caused by Pseudomonas putida.

본 발명에서 식품 조성물은 각종 식품류, 예를 들어, 음료, 껌, 차, 비타민 복합제, 분말, 과립, 정제, 캡슐, 과자, 떡, 빵 등의 형태로 제조될 수 있다. 본 발명의 식품 조성물은 독성 및 부작용이 거의 없는 식물추출물로 구성된 것이므로 예방 목적으로 장기간 복용 시에도 안심하고 사용할 수 있다.The food composition of the present invention may be prepared in the form of various foods such as beverages, gums, tea, vitamin complexes, powders, granules, tablets, capsules, confectionery, rice cakes and breads. Since the food composition of the present invention is composed of a plant extract having little toxicity and side effects, it can be safely used for prolonged use even for prophylactic purposes.

여기서, 상기 식품 조성물이 음료 형태로 제조되는 경우 지시된 비율로 상기 식품 조성물을 함유하는 것 외에 특별한 제한점은 없으며 통상의 음료와 같이 여러가지 향미제 또는 천연 탄수화물 등을 추가 성분으로서 함유할 수 있다. 즉, 천연 탄수화물로서 포도당 등의 모노사카라이드, 과당 등의 디사카라이드, 슈크로스 등의 및 폴리사카라이드, 덱스트린, 시클로덱스트린 등과 같은 통상적인 당 및 자일리톨, 소르비톨, 에리트리톨 등의 당알콜 등을 포함할 수 있다. 상기 향미제로서는 천연 향미제(타우마틴, 스테비아 추출물(예를 들어 레바우디오시드 A, 글리시르히진등) 및 합성 향미제(사카린, 아스파르탐 등) 등을 들 수 있다. Here, when the food composition is prepared in a beverage form, there are no particular limitations other than those containing the food composition at the indicated ratios and may contain various flavors or natural carbohydrates such as ordinary beverages as an additional ingredient. That is, natural carbohydrates include monosaccharides such as glucose, disaccharides such as fructose, sucrose and the like and sugar sugars such as polysaccharide, dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol can do. Examples of the above-mentioned flavors include natural flavors (such as tau martin and stevia extract (for example, rebaudioside A and glycyrrhizin) and synthetic flavors (for example, saccharine and aspartame).

그 외 본 발명의 식품 조성물은 여러 가지 영양제, 비타민, 광물(전해질), 합성 풍미제 및 천연 풍미제 등의 풍미제, 착색제, 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알콜, 탄산 음료에 사용되는 탄산화제 등을 함유할 수 있다.In addition, the food composition of the present invention can be used as a flavoring agent such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, colorants, pectic acid and its salts, alginic acid and its salts, , a pH adjusting agent, a stabilizer, a preservative, a glycerin, an alcohol, a carbonating agent used in a carbonated drink, and the like.

이러한 성분은 독립적으로 또는 조합하여 사용할 수 있다. 이러한 첨가제의 비율은 그렇게 중요하진 않지만 본 발명의 식품 조성물 100 중량부 당 0.1 내지 약 50 중량부의 범위에서 선택되는 것이 일반적이다.These components may be used independently or in combination. The proportion of such additives is not so critical, but is generally selected in the range of 0.1 to about 50 parts by weight per 100 parts by weight of the food composition of the present invention.

본 발명에서 제공하는 신규한 박테리오파지는 강력한 항균제인 카바페넴 내성 슈도모나스 균속에 대하여 특이적 사멸능을 가지므로, 상기한 카바페넴 내성 슈도모나스 균속에 의해 유발되는 질환의 예방 및 치료 목적으로 활용할 수 있다.The novel bacteriophage provided in the present invention has a specific killing activity against carbapenem-resistant Pseudomonas sp., A potent antimicrobial agent, and thus can be utilized for the prevention and treatment of diseases caused by carbapenem-resistant Pseudomonas sp.

도 1은 실시예 1에서 카바페넴에 대해 내성을 갖는 슈도모나스 푸티다 균주를 나타낸 것이다.
도 2는 실시예 4에서 카바페넴에 대해 내성을 갖는 슈도모나스 푸티다에 대한 용균성 박테리오파지 YMC11/06/C171 PPU BP의 흡착능을 그래프로 나타낸 것이다.
도 3은 실시예 4에서 카바페넴에 대해 내성을 갖는 슈도모나스 푸티다에 대한 용균성 박테리오파지 YMC11/06/C171 PPU BP의 1단 증식 곡선을 나타낸 것이다.
도 4는 실시예 5에서 카바페넴에 대해 내성을 갖는 슈도모나스 푸티다에 대한 용균성 박테리오파지 YMC11/06/C171 PPU BP의 전체 유전체 서열분석(whole genome sequence analysis)을 나타낸 것이다.
도 5는 실시예 5에서 카바페넴에 대해 내성을 갖는 슈도모나스 푸티다에 대한 용균성 박테리오파지 YMC11/06/C171 PPU BP의 전체 유전체 서열분석 중 ORF(Open reading frame) 분석 결과를 나타낸 것이다.
도 6은 실시예 6에서 생체 외(in vitro)에서 박테리오파지 YMC11/06/C171 PPU BP의 카바페넴에 대해 내성을 갖는 슈도모나스 푸티다에 대한 용균능을 분석하여 나타낸 것이다.Fig. 1 shows a Pseudomonas putida strain resistant to carbapenem in Example 1. Fig.
Figure 2 is a graphical representation of the adsorptivity of the bacteriophage YMC11 / 06 / C171 PPU BP against Pseudomonas putida resistant to carbapenem in Example 4.
Fig. 3 shows the single-step growth curve of the bacteriophage YMC11 / 06 / C171 PPU BP against Pseudomonas putida resistant to carbapenem in Example 4. Fig.
Figure 4 shows a whole genome sequence analysis of the bacteriophage YMC11 / 06 / C171 PPU BP against Pseudomonas putida resistant to carbapenem in Example 5.
FIG. 5 shows ORF (open reading frame) analysis results of the whole genome sequence analysis of the bacteriophage YMC11 / 06 / C171 PPU BP against Pseudomonas putida having resistance to carbapenem in Example 5. FIG.
Figure 6 shows the potency of Pseudomonas putida resistant to carbapenem of bacteriophage YMC11 / 06 / C171 PPU BP in vitro in Example 6.

이하, 본 발명을 하기의 실시예에 의해 상세히 설명한다. 단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 의해 한정되는 것은 아니다. Hereinafter, the present invention will be described in detail with reference to the following examples. However, the following examples are illustrative of the present invention, and the contents of the present invention are not limited by the following examples.

실시예Example 1: One: 카바페넴Carbapenem 내성 임상 검체 분리 균주 수집 및 균주 은행 구축 Collection of isolates of resistant clinical specimens and establishment of strain bank

(1) 임상 검체 분리 균주 수집: 인덱스 균주 은행 구축(1) Collection of isolates of clinical specimens: Establishment of index strain bank

세브란스 병원의 환자들로부터 호흡기 감염증, 패혈증 및 농양 검체를 수집하였으며 각 검체에 대한 임상적 위중도 평가 기준은 다음과 같다. 호흡기 검체는 그룹 4,5,6으로 제한하였고, 패혈증 검체는 3쌍 배양 중 2쌍 이상에서 증식하였으며, 농양 검체는 심부농양 주사기 아스피레이트에 제한하였다. 상기 검체로부터 슈도모나스 균속에 속하는 병원균을 배양 분리하였고, 균주의 동정을 위해 전통적 방법을 통한 생화학적 동정 혹은 16s rRNA/ITS/rpoB 염기서열 분석을 통한 분자역학적 방법을 실시하였다. 감수성 결과는 Clinical and Laboratory Standards Institute (CLSI, 2009)를 기준으로 판독하였다. 감수성 시험은 뮬러-힌튼(Mueller-Hinton) 아가를 사용하여 외기 37℃에서 하룻밤 동안 배양하는 CLSI 디스크 확산 시험방법을 사용하였다. 슈도모나스 균속에 대한 시험 항균제는 아미카신(Amikacin), 아즈트레오남(Aztreonam), 세즈타지딤(Ceftazidime), 시프로플록사신(Ciprofloxacin), 콜리스틴(Colistin), 세페핌(Cefepime), 젠타마이신(Gentamicin), 이미페넴(Imipenem), 레보플록사신(Levofloxacin), 메로페넴(Meropenem), 피페라실린(Piperacillin), 피페라실린/타조박탐(Piperacillin/Tazobactam), 티카실린(Ticarcillin), 토브라마이신(Tobramycin)을 사용하였다. 카바페넴 내성 균주을 선별하기 위해 이미페넴 호지 테스트(Imipenem Hodge test)를 통해 카바페넴아제를 발현하는 균주를 선별하였다. 이 때, 메탈로-β-락타마제(metallo-β-lactamase)와 세린-β-락타마제(serin-β-lactamase)를 포함한 카바페넴아제(carbapenemases)의 PCR 스크리닝 프라이머 세트는 하기 표 1에 나타내었다.Respiratory infections, sepsis, and abscesses were collected from patients at Severance Hospital. Clinical severity ratings for each sample were as follows. Respiratory specimens were restricted to groups 4,5,6, and sepsis samples proliferated in more than two pairs of triplicate cultures. Abscess specimens were restricted to deep abscess syringe aspirate. Pseudomonas species belonging to the genus Pseudomonas were isolated from the specimens. Molecular epidemiological methods were performed by biochemical identification or 16s rRNA / ITS / rpoB sequencing. Sensitivity results were read based on the Clinical and Laboratory Standards Institute (CLSI, 2009). The susceptibility test was performed using a CLSI disk diffusion test method in which Mueller-Hinton agar was used to culture at ambient temperature at 37 ° C overnight. Test antimicrobial agents against Pseudomonas spp. Include Amikacin, Aztreonam, Ceftazidime, Ciprofloxacin, Colistin, Cefepime, Gentamicin, , Imipenem, Levofloxacin, Meropenem, Piperacillin, Piperacillin / Tazobactam, Ticarcillin and Tobramycin were used. Respectively. In order to screen for carbapenem-resistant strains, strains expressing carbapenemase were selected through the Imipenem Hodge test. At this time, a PCR screening primer set of carbapenemases including metallo-beta-lactamase and serine-beta-lactamase is shown in Table 1 below .

타겟(target)Target 프라이머(primer)Primer 프라이머 서열(5'-3')The primer sequence (5'-3 ') 결과물 크기Product Size blaVIMblaVIM VIM2FR-FVIM2FR-F ATGTTCAAACTTTTGAGTAAGATGTTCAAACTTTTGAGTAAG 801bp801bp VIM2FR-RVIM2FR-R CTACTCAACGACTGAGCGCTACTCAACGACTGAGCG blaIMP bla IMP IMP1-FIMP1-F CATGGTTTGGTGGTTCTTGTCATGGTTTGGTGGTTCTTGT 450bp450bp IMP1-RIMP1-R ATAATTTGGCGGACTTTGGCATAATTTGGCGGACTTTGGC blaSIM-1 bla SIM-1 SIM-1 MP FSIM-1 MP F TACAAGGGATTCGGCATCGTACAAGGGATTCGGCATCG 571bp571bp SIM-1 MR RSIM-1 MR R TAATGGCCTGTTCCCATGTGTAATGGCCTGTTCCCATGTG blaSPM-1 bla SPM-1 SPM-1-ASPM-1-A CTGCTTGGATTCATGGGCGCCTGCTTGGATTCATGGGCGC 784bp784bp SPM-1-BSPM-1-B CCTTTTCCGCGACCTTGATCCCTTTTCCGCGACCTTGATC blaGIM-1 bla GIM-1 GIM1-FGIM1-F ATTACTTGTAGCGTTGCCAGCTATTACTTGTAGCGTTGCCAGCT 726bp726bp GIM1-RGIM1-R TAATCAGCCGACGCTTCAGTAATCAGCCGACGCTTCAG blaAIM-1 bla AIM-1 Yong MBLFYong MBLF GGCATTGATGCGCATCAAGGGGCATTGATGCGCATCAAGG 726bp726bp Yong MBLRFYong MBLRF GAAGACGTCGTCCGAGATCGGAAGACGTCGTCCGAGATCG blaNDM-1 bla NDM-1 KP05506 M13 R3KP05506 M13 R3 CAATATTATGCACCCGGTCGCAATATTATGCACCCGGTCG 726bp726bp KP05506 M13 R2KP05506 M13 R2 ATCATGCTGGCCTTGGGGAAATCATGCTGGCCTTGGGGAA blaHKM-1 bla HKM-1 KHM-1 FKHM-1 F GGTATGCGCTGACGATTCATGGTATGCGCTGACGATTCAT 526bp526bp KHM-1 RKHM-1 R TACCGCATGGCTAAGATTGCTACCGCATGGCTAAGATTGC blaSME bla SME SME1-3 FSME1-3 F AGCGGTTCCCTTTATGCAGTAGCGGTTCCCTTTATGCAGT 661bp661bp SME1-3 RSME1-3 R CGTGATGCTTCCGCAATAGTCGTGATGCTTCCGCAATAGT blaIMI, blaNMC bla IMI, bla NMC IMI NMC FIMI NMC F CGATTGGGTAGTTGGCGATACGATTGGGTAGTTGGCGATA 173bp173bp IMI NMC RIMI NMC R CGATTCTTGAAGCTTCTGCGCGATTCTTGAAGCTTCTGCG blaGES bla GES GES 2456 FGES 2456 F CGAAAAAGCAGCTCAGATCGCGAAAAAGCAGCTCAGATCG 761bp761 bp GES 2456 RGES 2456 R TGTCCGTGCTCAGGATGAGTTGTCCGTGCTCAGGATGAGT blaKPC bla KPC KPC 1-4 FKPC 1-4 F TGGACACACCCATCCGTTACTGGACACACCCATCCGTTAC 515bp515bp KPC 1-4 RKPC 1-4 R GACGGCCAACACAATAGGTGGACGGCCAACACAATAGGTG

(단, 세포불멸화(immortalization)은 -70℃ 초저온 냉동고에서 10% 글리세롤 스톡(glycerol stock)으로 수행하였다.)(However, cell immortalization was performed with 10% glycerol stock in a -70 ° C cryocooler.)

수집된 슈도모나스 푸티다(Pseudomonas putida ) 균주가 카바페넴에 대한 내성을 나타내었다(도 1). The footage collected Pseudomonas (Pseudomonas putida ) showed resistance to carbapenem (Fig. 1).

단, 도 1에서 S, I 및 R은 항균제에 대한 감수성을 평가한 결과로, 'S'는 민감(Susceptible), 'I'는 중간(Intermediate), 'R'은 내성(Resistant)를 의미한다.In FIG. 1, S, I and R are the results of evaluating the susceptibility to the antimicrobial agent, wherein 'S' means susceptible, 'I' means intermediate, and 'R' means resistant .

실시예Example 2. 파지 은행 구축을 위한 검체 수집 및 파지 분리 정제 2. Sample collection and phage separation purification for phage bank establishment

(1) 파지 은행 구축을 위한 검체 수집(1) Collection of samples for phage bank establishment

세브란스 병원의 오수 처리시설에서 최초 침전지를 거친 후 부유물질 및 침사물이 제거된 처리수 2L를 채취하였다. 이는 화학 처리 시설 전 단계의 오수로 제한하였다. 수집한 시료에 1L당 염화나트륨 58g을 첨가한 후 10,000g에서 10분간 원심 분리하여 200nm 밀리포어 필터로 여과하였다. 얻어진 여과액에 폴리에틸렌글리콜(PEG, 분자량 8000)을 10% V/V으로 첨가하고 4℃에서 12시간 동안 냉장 보관하였다. 12시간 냉장 보관된 여과액을 12,000g에서 20분간 원심 분리하여 침전물을 파지 희석 완충액 (SM 완충액)에 재부유하여 냉동 보관하였다. 이를 3회 반복하여 300mL의 박테리오파지 부유액을 채취하였다. In the sewage treatment facility of Severance Hospital, 2L of treated water from which the suspended matter and sediment were removed after the first sedimentation basin was collected. This was limited to sewage at the pre-stage of the chemical treatment facility. 58 g of sodium chloride per liter was added to the collected samples, and the mixture was centrifuged at 10,000 g for 10 minutes and filtered through a 200 nm Millipore filter. Polyethylene glycol (PEG, molecular weight 8000) was added to the obtained filtrate at 10% V / V and stored at 4 占 폚 for 12 hours in a refrigerator. The 12-hour-refrigerated filtrate was centrifuged at 12,000 g for 20 minutes and the precipitate was resuspended in a digestion buffer (SM buffer) and stored frozen. This was repeated three times to collect 300 mL of the bacteriophage suspension.

(2) 용균성 파지 선별 및 정제(2) Screening and purification of bacteria-resistant phage

① 스팟 테스트(Spot test) 및 플라그 분석(Plaque assay)을 이용한 파지 정제 ① Phage purification using spot test and plaque assay

용균성 파지의 분리 정제는 스팟 테스트(Spot Test)법 (Mazzocco A et al. In Bacteriophages, Clokie and Kropinski AM, eds. Humana Press. 2009)으로 실행하였다. 확보된 균주를 맥콘키 한천배지에서 접종 후 외기 35℃에서 하룻밤 동안 배양하였다. 배양 후, 투명한 플라크 형성을 보고 파지에 감수성인 균주를 선별하였다. 감수성인 균주를 맥콘키 한천배지에 접종하여 35℃에서 12시간 동안 배양하였다. 살린 1ml 튜브에 McFarland 0.5 탁도로 각 균주의 현탁액 제조하고 H 탑 아가 (3 ml), 감수성 박테리아 100ul 및 파지 용액 (각각 1ul, 10ul 및 50ul)을 섞어 LB 아가에 도포한 후, 35℃에서 12시간 동안 배양하였다. 플라크 관찰한 후에 파스퇴르 파이펫으로 플라크를 채취하여 SM 완충용액에 희석하고, 다시 감수성인 균주 현탁액을 이용하여 3회 반복 정제하였다. 이렇게 얻어진 순수한 박테리오파지는 SM 완충용액에 희석하고 다시 감수성인 균주 현탁액을 이용하여 3회 반복 정제하였다. 이렇게 얻어진 순수한 박테리오파지는 SM 완충용액에 희석하여 보관하였다.The separation and purification of the soluble phage was carried out by the spot test method (Mazzocco A et al. In Bacteriophages, Clokie and Kropinski AM, eds Humana Press. The obtained strains were inoculated on a McConki agar medium and incubated overnight at ambient temperature of 35 ° C. After culture, transparent plaque formation was observed, and strains susceptible to phage were selected. The susceptible strains were inoculated on a McConki agar medium and cultured at 35 DEG C for 12 hours. (1 ml, 10 ul and 50 ul, respectively) were added to the LB agar, and the mixture was incubated at 35 ° C for 12 hours Lt; / RTI > After plaque observation, plaques were taken with Pasteur pipette, diluted in SM buffer, and further purified three times using a susceptible strain suspension. The pure bacteriophage thus obtained was diluted in SM buffer solution and subjected to repeated purification three times using a susceptible strain suspension. The pure bacteriophage thus obtained was diluted with SM buffer solution and stored.

② 용균성 파지의 용균역가 측정② Measuring the lytic activity of the bacterium phage

실시예 1에서 확인한 카바페넴 내성 슈도모나스 푸티다 균주를 맥콘키 한천배지에서 접종하여 배양한 후, 정제된 박테리오파지 YMC11/06/C171 PPU BP를 도말된 각각의 균주에 5ul로 접종하여 플라그 형성을 확인하고, 역가 범위를 확인하였다. 그 결과, 카바페넴 내성 슈도모나스 푸티다 균주만을 특이적으로 용균시킨 것을 확인할 수 있었다.The carbapenem-resistant Pseudomonas putida strain identified in Example 1 was inoculated and cultured on a McConki agar medium, and then the purified bacteriophage YMC11 / 06 / C171 PPU BP was inoculated at 5 ul into each of the strains smoothed to confirm plague formation , And the potency range was confirmed. As a result, it was confirmed that only the carbapenem-resistant Pseudomonas putida strain was specifically lysed.

실시예Example 3. 3. 카바페넴Carbapenem 내성 슈도모나스 Resistant Pseudomonas 푸티다에Putida 대한 About 용균성Solvent strength 박테리오파지의 전자 현미경 분석 Electron microscopic analysis of bacteriophages

정제된 박테리오파지를 감수성 균주 배양 배지(20ml LB 배지)에 접종 및 배양한 뒤 220nm 밀리포어 필터로 여과하고, 상청액에 폴리에틸렌글리콜(MW 8,000)을 10%(w/v)의 양으로 첨가한 후 밤새 냉장 보관하였다. 이후 12,000g의 조건으로 20분 동안 원심 분리한 뒤, 에너지 여과 투과 전자현미경(Energy-Filtering Transmission Electron Microscope)을 이용하여 박테리오파지의 형태를 분석했다. The purified bacteriophage was inoculated and cultured in a susceptible strain culture medium (20 ml of LB medium) and then filtered with a 220 nm Millipore filter. To the supernatant was added polyethylene glycol (MW 8,000) in an amount of 10% (w / v) And refrigerated. After centrifugation at 12,000 g for 20 minutes, the shape of the bacteriophage was analyzed using an Energy-Filtering Transmission Electron Microscope.

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실시예Example 4. 슈도모나스 4. Pseudomonas 푸티다에Putida 대한 박테리오파지 Korean bacteriophage YMC11YMC11 /06/C171 / 06 / C171 PPUPPU BP의 흡착능 및 1단 증식 곡선(one-step growth curve) 분석 BP adsorption and one-step growth curve analysis

카바페넴 내성 슈도모나스 푸티다를 OD 값이 0.5가 되도록 배양한 뒤, 슈도모나스 푸티다에 정제된 박테리오파지 YMC11/06/C171 PPU BP를 MOI 0.001로 넣고 상온에서 배양하였다. 100ul 시료를 1, 2, 3, 4, 5분에 1ml씩 채취하여 LB 배지에 희석한 뒤 플라그 분석을 통해 상기 박테리오파지의 흡착능을 평가하였다.The carbapenem-resistant Pseudomonas putida was cultured to an OD value of 0.5, and the purified bacteriophage YMC11 / 06 / C171 PPU BP was added to Pseudomonas putida at an MOI of 0.001 and cultured at room temperature. 1 ml of each 100 μl sample was taken at 1, 2, 3, 4, and 5 minutes, diluted in LB medium, and then adsorbed to the bacteriophage by plaque analysis.

또한, 카바페넴 내성 슈도모나스 푸티다를 OD 값이 0.3이 되도록 배양한 뒤, 4에서 5분 동안 7,000g로 원심 분리하여 세포를 침강시킨 후, 0.5ml의 LB 배지에 희석시키고, 정제된 박테리오파지 YMC11/06/C171 PPU BP를 MOI 0.001(titer 10⁸pfu/cells)를 넣고 37에서 5분 동안 배양하였다. 배양된 혼합 시료를 13,000g에서 1분 동안 원심 분리하여 얻어진 펠렛을 10ml의 LB 배지에 희석시키고 37에서 배양하였다. 배양 도중 10분 마다 시료를 채취하여 플라그 분석을 통해 상기 박테리오파지의 1단 증식 곡선을 평가하였다.In addition, carbapenem-resistant Pseudomonas putida were cultured to an OD value of 0.3, followed by centrifugation at 7,000 g for 4 to 5 minutes to precipitate the cells. Then, the cells were diluted in 0.5 ml of LB medium, and the purified bacteriophage YMC11 / 06 / C171 PPU BP was incubated with MOI of 0.001 (titer 10 ⁸ pfu / cells) at 37 for 5 min. The cultured mixed sample was centrifuged at 13,000 g for 1 minute, and the resulting pellet was diluted in 10 ml of LB medium and cultured at 37. Samples were collected every 10 minutes during culturing, and the 1-fold growth curve of the bacteriophage was evaluated through plaque analysis.

그 결과, 박테리오파지 YMC11/06/C171 PPU BP의 접종 후 1분 이내에 박테리오파지의 약 97% 정도가 슈도모나스 푸티다에 대하여 흡착하는 것을 확인할 수 있었고(도 2), 25 PFU/감염 세포의 높은 버스트 사이즈(burst size)를 나타내었다(도 3).As a result, it was confirmed that about 97% of the bacteriophages were adsorbed to Pseudomonas putida within 1 minute after the inoculation of the bacteriophage YMC11 / 06 / C171 PPU BP (Fig. 2) and 25 PFU / burst size) (Fig. 3).

실시예Example 5. 분리된 박테리오파지 5. Isolated bacteriophage YMC11YMC11 /06/C171 / 06 / C171 PPUPPU BP의 전체 게놈 서열 분석 Whole genome sequencing of BP

전 유전체 서열분석을 통해 YMC11/06/C171 PPU BP의 전 유전체 (45,417bp. 49 ORF, G+C 함량 60.35%)를 분석하였다(도 4). YMC11/06/C171 PPU BP의 전 유전체와 유사성을 보이는 파지는 존재하지 않는다. The entire genome of the YMC11 / 06 / C171 PPU BP (45,417 bp, 49 ORF, G + C content of 60.35%) was analyzed by the whole genome sequence analysis (FIG. YMC11 / 06 / C171 PPU There is no phage showing similarity to the entire genome of BP.

상기한 YMC11/06/C171 PPU BP의 유전체에 있어서, 49개의 ORF 중 대부분 가설 단백질(hypothetical protein)로 분석되었고, 20개의 ORF(ORF2, 3, 4, 5, 6, 11, 25, 26, 29, 30, 32, 35, 36, 38, 42, 45, 46, 47, 48, 49)만이 기능 단백질이었으며(도 5), 이들 20개의 ORF의 핵산 서열은 서열번호 2 내지 21로 표시된다. In the genome of YMC11 / 06 / C171 PPU BP described above, most of the 49 ORFs were analyzed as hypothetical proteins and 20 ORFs (ORF2, 3, 4, 5, 6, 11, 25, 26, 29 (SEQ. ID. Nos. 2 to 21) of these 20 ORFs were found to be the functional proteins (FIG. 5), and 30, 32, 35, 36, 38, 42, 45, 46, 47,

또한, 49개의 ORF 중 ORF2, 11 및 18은 용균 효과를 나타내는 특이적 유전자 서열에 해당한다. Of the 49 ORFs, ORFs 2, 11, and 18 correspond to specific gene sequences exhibiting a lytic effect.

실시예Example 6. 생체 외 6. In vitro 카바페넴Carbapenem 내성 슈도모나스 Resistant Pseudomonas 푸티다에Putida 대한 박테리오파지의 용균능 검증 Application of Bacteriophage in Korea

카바페넴 내성 슈도모나스 푸티다 1 X 10⁹ CFU/ml에 준비된 박테리오파지 YMC11/06/C171 PPU BP를 1 X 10⁸ CFU/ml(MOI: 0.1), 1 X 10⁹ PFU/ml(MOI: 1), 1 X 10¹⁰ PFU/ml(MOI: 10)의 양으로 각각 처리하고 시간 별로 OD 값(파장 600nm)을 측정하였다. 단, 대조군으로는 PBS+SM 버퍼를 처리하였다. Cover penem-resistant Pseudomonas footage is 1 X 10 ⁹ CFU / ml prepared in bacteriophage YMC11 / 06 / C171 PPU BP to ^{1 X 10 8 CFU / ml (} MOI: 0.1), 1 X 10 9 PFU / ml (MOI: 1), 1 × 10 ¹⁰ PFU / ml (MOI: 10), and the OD value (wavelength: 600 nm) was measured with time. However, the control group was treated with PBS + SM buffer.

대조군과 비교할 때, 슈도모나스 푸티다에 대하여 박테리오파지 YMC11/06/C171 PPU BP를 처리한 경우 OD값이 감소하는 것을 볼 수 있었고, 특히 MOI 값이 증가할수록 OD값이 더욱 감소하는 것을 볼 수 있었다. Compared with the control group, OD value decreased with treatment with bacteriophage YMC11 / 06 / C171 PPU BP against Pseudomonas putida, and OD value decreased with increasing MOI value.

이를 통하여, 본 발명에 따른 박테리오파지 YMC11/06/C171 PPU BP는 카바페넴 내성 슈도모나스 푸티다에 대하여 용균성을 갖는 것을 알 수 있다(도 6).Thus, it can be seen that the bacteriophage YMC11 / 06 / C171 PPU BP according to the present invention has a tolerance to carbapenem-resistant Pseudomonas putida (FIG. 6).

한국생명공학연구원Korea Biotechnology Research Institute KCTC18380PKCTC18380P 2015042920150429

<110> Industry-Academic Cooperation Foundation, Yonsei University <120> Lytic bacteriophage specific for Pseudomonas genus resistant to antibiotics <130> DPB155798 <160> 21 <170> KopatentIn 2.0 <210> 1 <211> 45417 <212> DNA <213> Pseudomonas putida phage <400> 1 tgcacaagcg gccaaatcct tcaatgagat tgctctgcag aagactgccc tgtccgccca 60 gatcgcaact gcccgcctag cggtgcagcg cgagggcgag caggtgaagt ctgcgcgggg 120 gctacaggcg gcgggcacgg acaccatggg tgcatccgta gcccaaggcc tgctggatgt 180 ggacatgaag atttccgagg cgcagcagga gctgctgtac aacggcaccc ttacggatca 240 gtcgttgaat gctcaggcgg ccaatgtggc tgacagcgcc ggcatgtccc ttcgcacccc 300 cactgccgtg agcaacaagt gggcacccat ccttgggcag gccgtggcca gcttcgggat 360 gtccctgttt gagaacaaag cccgcaccgg tagcttcacc gggcggaacc aaggtctata 420 aggaggcatc atgccagtac gccaagatgt acaaggcggc ccgcgggctg ccggtcttga 480 gggttatcag agcccgggag ccgcccgtgc gcagttccaa ggcccagacc tcagcggcgt 540 acagcgcgat gccgcagacc aagccaactt ctggacccgg ttgatcggcc gtggggcgaa 600 cgccctgcag gctatggacg agaaagaccg ggcccgggcc tatttagagg gtcagcaaga 660 cagcgccatg ggcaaggccc gcgctgaagt gtatggctca atgcagtccg actacgagca 720 aggctataac cgcgccacca ttggcagcca gttggcgaag taccagctgg gcgtgcagca 780 agccgccatc gaacatgtga actcgggcaa gaccccagag gagttccagc agttcgtgga 840 ggaggagacg aactccctgc tgaacgcagc cggcactcaa ggccttgatc tgaacgagaa 900 ggactggcag gcgtggctga ccggcgtgca gagcacccgg gccaccgccg ctgatctgtt 960 ccagaccaag agcgtagagc gtgcccagta catgaagggg caggccatcg ccgccgaggg 1020 caacgccagc attgctacct tcatggctgc cgatgaggcg ggtaacccac tgcaggcgct 1080 gggcgggatc acctcccaca ttgcccgcgt gtactccgac aacaccttgt cgcctcagca 1140 aaaggacggg gcccttgcgg acttcgctgg ccaactcatg gcaagcgcta agtcgtccgc 1200 cgccgttgag gcgctgggct cgtacttcca agagtcggcc gagttcaaat ccctgcctac 1260 tcaggtacag acgcaggtta tcaacggcgc ccagcgggcg tacgagcagc gtgcagccga 1320 cgagtcgggt gcggtgtact cgtacgtgtc gaacgtgcgc gctgtggcca acccggatga 1380 gctggagcgc cagttcccca tggcgtcctt tattagcacc ctgaacgagg cccagcgggc 1440 tcaccgcatc actccggccc agatgtacgg tatggtggag gaggagaaca ctcgccgcct 1500 gaagctcggc aaggctgccg cattccagca gaacctgctg cacggcacca ccatgtccga 1560 tatggcagct ggtaccggtc agactctcgg caagatcaag actgccctca cgcaggtata 1620 cgccaccagc aacggtggct acagcggcgg cgggctggcg ctcatgcagc gtggtctgca 1680 gagtggggcg caggacatca cagctgtggg tatcgagatg ctgcagcaag acgcacagtc 1740 cctcggcagc atcgacagcc ggaacctgaa gcgcgatgct gacgggcagg ccctgtaccc 1800 ggctaccgtg gtgaactcgc tgacgaacct gaagcgtgcg tacgacgctg cacaacgagc 1860 cggcaacaac gtgcaggctt ctcagctcat tgctgggctg cccgacgctg tagcctatgg 1920 catccgccaa gccagcgacg agaatagcat tgcggacgtt gtgtaccgcc gggccgatga 1980 tatcgcctcc gggcgcgtgg tctccctgcc ggctgccatg cccaaggaaa tgctcgccac 2040 tgtggacgat gtatccgccg gcatgttcga cacgaacctg acccagaagg gcgcggctcg 2100 gaacatcctc ggcatccagt cgtacgtgtt caccagcaag gaagatgccc gggcgcagga 2160 agcccgcttg gcgcaggtga acggtgccct cggtgaggag tacacctcac tgtaccagca 2220 aggccgtctg ccagcgctcg ccggcgacga cctgaagaac tggctggtgg gtcgtgtggc 2280 tgcccgtacc gtgcgtgtag acgatggcac cgacaacggc acactgctga tcctgcctaa 2340 cgtggccaac cgcgcccaga cctttggcag tgaggacaac aacatcatcg gccaaggcct 2400 gaaggactct gttgacgagt tccgtgcagc caatcccggc gctgtatccg tccagatgcg 2460 gtacgacccc atggctggcg agatcgtgtt cagcggcact gacaaggaca acacggtgct 2520 caccacttcg tacgccgtgc cggtcgatca ggttgtacgc caagtgcgtg cagttgagac 2580 tcgcctgacc gcgaacggta atggtaacag catgggctcg ctcgctgtgc ccggcgccgg 2640 gtttgtgcag tttaatacgg ccaacgccta cggaatcgag ccgcaagtgt atcaaaatgc 2700 agttacccag ctggtctcat acgagggcta caccgacacc aaaggcttca gcatcctggc 2760 cacccaccca accacgggtg cggccctgaa cgaggccaag tacgtgaagc agcccaccga 2820 cacgccacag gtcgctgcga acaagctgca actgtacatc caagacaagg tgcttccgcc 2880 tgtcatggct gagttgccgc agttcaacgc cctgcctgag cacatccgtc agcaggtgtt 2940 ccagcagctg gtggagacga cgtatcacgc cggtaacgcc aaggccttcg gggacatcct 3000 gaagaaggcg ctggatggtg acacactggg ggcctaccaa gacttccgcg agtccgccct 3060 gtacaaggac gctgggccta agtcccgccg caaccgtgac cgactgcaaa cccttgacgc 3120 tgtatcccaa tacagccgcg tgcaacgcac cggctactga actggaggtt ccaatgagca 3180 tgatccgttt acgccctgac gaagaccttg ctgtcgcacc gggcaccgcc ccgactgtcg 3240 gcggcgggtt cgtctccggc gctgagaaca gcttactgga acccggtaca gcaaacacct 3300 cggcgctgaa agtagcgccg acctcttctg cagagagtgc cgagggcgct ctccgcgaaa 3360 ccatcgcagc caaggcgctg cgtggtatcg agtacatgca aatcccaagc gaggaggggt 3420 tcgaccctgc agccgcgctg ggcgacaaac tcggcgcctt cactgaggaa gagctggagt 3480 tccttggcga tgcccgctcc agcaccgaac tggcccagcg cctcggtcaa gtgacccaga 3540 cccgccagaa cctgcaggac atggccgcca accctgtaac cgccttcgcc gcctcgatgc 3600 tggacatcga cgctgtgatc ggcctcgggg tagctggtgc tgctacgcgc gcgggccgta 3660 ccgcgcgtct ggttgccggc ctggccgcta acactgcggt actgggcgct gcatccgagg 3720 gcggtgagat caccccgctg gatgtcatcg gcacatcgct ggtcgtagcg ctgggcgcaa 3780 tccccaccgt acgccgtgca gcaaccgttg ccgaggacgc ggctcaggac ctcccaacag 3840 gcgcgcgtag cgttcctgac gttgaggagg tagtaccccc acgccccgct gctgaagtcg 3900 ctccagatgt cgtccctgac gctcaggtgg ccccttcccg catccctgac ccagactatg 3960 aagtccctac ccctgacatg acgactgtac gcccgtacgt cgaagtgaat cgggtcaagg 4020 gtcaagccac catccgcacc agtactgcga accttgtggg cgccacactg gccaatgcca 4080 gcgacctgcc cgagggtacc cgccttctgg gtgtagcctt gtacgatagc atgaagctgg 4140 acggcgacgt gcccctgatc atcacccgtg gcaagggtcg atcgaacgtc cgcctgtggc 4200 cagacggccg cctgcagacc accatgcgca cagccgaggg cggggcacct accctgactg 4260 aggccgtgca gaacatgagc acgtacgaga agaccgttaa cctgcacgag gcagcacacg 4320 ccaagaccat cacagcgatt gccctgttcc aacagggtaa gctctcggct ggtccagtgt 4380 tcgacgcagt gcagcagatc gatcaaatcc gcaacgtcgt gcggggcaac atcagtaccc 4440 caaccctgcg tgagggcatc agcacttccg actggaagaa caacgtgctg tacggactca 4500 gcaacaccca cgagttcgtg tcccagctgt tcaacagcga gtccttccgc aaggtactgc 4560 accagatccc tatgcccggt gagccccgct cagtgtggtc tagcttggtg cagaaggttg 4620 tgaccgcctt cactggcaag ggtcctaccg acaccgcgct gacccgcttg gtggctgcat 4680 tcgaagacct attgcaacag ccctctgtga ctgacgcgca gttcgtgaag gcgtacaaca 4740 aggccgacgt gccgcgtacc caatcggccg cgctggctaa ctccccagac atccagacca 4800 tgttcacccg caccgggaac tcgttgaatc aggccttcgc cttgttcgac aagattcgcg 4860 ccatcggccc acgggccagc acccttgcgg aacaactggt tgtcgatgcc actgggaact 4920 cggctacgtc tgctgcgcat tacgcccgca ctgcccactt ggctgccaac gtggccgctg 4980 cgcaggttga tgctgctgtg cgtcaggccc tgtccgccca aggctggact atgttctctc 5040 gcctgcgcaa cccagctgca ttccgggctg cacagcagga gctgtccggc aaggtgtacg 5100 acgccctgtc cgataaccat gcccgcttca tgtcgggcgg caccgtgaac cctcatccag 5160 acgcagccgt gcagcgcatc gtcgatgcct tcgccagctc ccgctgggct gaggaccagc 5220 tggcccgcat caaggcctct ggcatgaccg gtgccgacac catcgaagcg tctccgtact 5280 acttgccacg ccgccacagc ggtaacaagg tgagcgactt cctccgtgcc aacccgggcg 5340 ttactcgggc ggacattgag gccatgtacg gtgagcagtt caagttaatg tttgcagacc 5400 gcggtatccg cccggacact gcccgcgcgc tgggtcgtca gatgctgcgg aacatggatg 5460 agcgagccgc cggcgtgcaa ggctaccgcc agcacatcgc aggtatgacc aacgacgaca 5520 tcgagttcgc tatgcgcaac gccggtatcg acgaggatca gatcaacatg ttcttgaaca 5580 ctgtgcagaa agcgggcgag caatccaaca ccgtgcggaa cctccgccgg cgcgtggact 5640 ttgacatgac ccgtgagtac gtgaccaaga gcggcgatgt gatcatgccg cagatgttcg 5700 tggacaagga tgtgctgggc ctgatggaag gctacagccg caccatgagt ggacgtattg 5760 ggctggccaa agccggtttc cctgacgtgc gtacgctcgc cggcgcagtc gatgaggctg 5820 ccgctgaggg cgcagaccca cgcgcagcgc gagccacgtt ggacaacact gtgaaccgac 5880 tgctgggcta ccccactggg gaagacgtgc ctgatatcct ccgcagcttt gctgtggcgt 5940 ctggtgcggt gcagctggcc aactctggta tctaccagct ggcagacact gccctgatga 6000 tcaaggagtt cggtattgcc aaggtactgc ggtccctgag ctccacagag tggggccgtg 6060 acgctttgaa gctggcacag gacccaacct acggctctcg cctccgcgac gttctggaag 6120 cacgcaacgt gctctcaggc cggtaccgga cagtgatgac ccacctcgac gacaacaccg 6180 atatcggaaa cctcggcatg ctgcaccaga acatccagca gctgggtcaa gtgacccgct 6240 tcgccaacgg catggagttc gtgcgccgcg ggcagtcgaa gatgatggcc gggctggtag 6300 gtgacaccgt ggatgacgcc atccgtggca acgacgcggc attcgagtcc ctgcagcgct 6360 ttggcatgac gcctgagctg cgtgcccggg ctaaggccgc gatggatgcc gaccctgaca 6420 tgcggatgtg gccagacagc ctgcgcctcg acatggagac tgtggcgcac aacatggctg 6480 acgcccttgt catggagaac cggttgggtg agcttcctgc ttggatgcag ttcagcgccc 6540 tcggcaagtt catcctgccg tacatgaact tcgtggctgg tacttggaac aagatcctgc 6600 gccgcacgta cgccaacgac ggccttgccg gcgtggctat gatgttcgcg tatcaactcc 6660 cgcttacaac cctctcgtca actgtagcgc ttgcgcaggg caacaaggag atcactcctc 6720 agaccctggc cgtgaacgtg ctgacccaaa tgccgctgat gtcgtggctc gggtacgctg 6780 tgaacatgat gactcaaggc ccgacgaaca gcattgctgc tcttgggttg gtcgataagg 6840 catactccgc taccgcgtca ctggtgcgtg gcgagcctga tccagagcaa atcatccgcg 6900 caaccccttt cctcgggatc atccccggta tccgcatcat ggcctcggcc ttgggcgagg 6960 acgaataagg agacacaatg aacgccatcc aaactgaggt atccgacggc agcttagtca 7020 gcgttaacgt cggactggaa tacttcgagc agtcggatat ctcggttttc ttggacttga 7080 gtatcgcgcc acttatcgaa ggcactgact acgcgtgggc caacgccacc cgcattgact 7140 tcctgacagg ccctgtggcc gcaggtgtgg cggtgacatt ggttcgaaac accgagaacg 7200 aggcaatgct gaacatctac gatggcggcg ccccgttcaa ccgattcacc cttgacgaga 7260 acttcttgca gctgctccgg ctgtcgcaag agttctccga gggcttaggc ttgaccggac 7320 tgcggcaggc cttggacatg cacggctacc gcatcttcaa ccttggtaac ccggtgaatg 7380 cacaggatgc taccaccaag gcgtacgtgg acctgctgat ccagaccctt gtggaatcgg 7440 gtaccggccc aatcggggcc gcctcgaatg tcgtgtacat cgaccctgaa ggccagctaa 7500 agaacctgca gcagctgtct ggtctcgatg gtgcggacct gatcggctgg aatggcacgg 7560 gcctgggtcc gttccttgaa gccggctact acgagccgca agggctggac cttaccggtg 7620 ttgtggacga gtctgcggta atcaacgccg cgctagtgaa gtacaagcgc gtgcgtctcc 7680 cggcaggcac cattaaggct aacgtgctcg tgccatccgg ctgtaccctg cggggcgccg 7740 gtaagacccg gctgaaccgt agcaccaagg tatgggccgc cggtggcacc accattatcg 7800 gctctgtagt ggcgtcgaac cagatcaact ggactgtcac cgacatgaac atcgacggct 7860 tcgccggcgg taacgctttt ggtgctcagg gcccatccac agagttcggg tacatcggtc 7920 gagtggcaat gcgcggtagt gaccacaaca tgatccttga gcagaacggc tctgatcttg 7980 caggccgctt cggcggcaat atcctcgtcg aggactgcga tgcgtatgat ggccctaacg 8040 ggtttgctgt gaagatgcgg aacgtgacct tccgccggtg ctacgcgtac gacatcacag 8100 tgcagggctt tgtggctgtg tctgacaaca tcaacggccc ggctatctac tcgcgcgcgc 8160 agaacgtgcg ctttgaggat tgtggcggcg acagctgcaa ccaaggcttt gtggtgtatt 8220 cccgcgactt ctaccacggg aatttccccg gcagctgggt cgccacggtg agtcccgcta 8280 ccgacatcta ctgggacggt acagtcacga acgtgacttc gggccaagta cacatcggtg 8340 acttcagctc ggacggcacc ttcgccattg tgttcaacaa cgagatcgag attggcggcg 8400 ggcactacca gcactccccg ctgtttgggg tgcggttcga ctgggcggca cgtccaaagg 8460 taaccggtgg ccacttctcc gggtgcccga acccgatcgt gcgcggcacg aacgtggtag 8520 acctcaagat tggtgatggg gtgacgcgct tcggtgccat caacccgggc atcctgtctg 8580 agcgcattat cgagagctca aataccagtg ccgtgaacat ccagaatgta cgccaagtgc 8640 tggtgttcca gaacaccgtg ccgaccgtgg ttacatccct cgtcacgcct gataccaacc 8700 acaccattga cgttgtgatt gacgacggcg ttacctcact acagctggcg ggcatcctgc 8760 tcaccggtcg gggcagccgg gcgcgcttcc agtggtcggc agataccagc acttggttgt 8820 gcctgatgag tggtagtgtg ctgccaccgg gcgagattgc ggttccgtac gccttgagca 8880 tgtctgccga ttggcgcgcc ctggcaaact ccatcgccat gaacggtaac atcaacctgc 8940 tggacgtacc tgtggcgaac atcgccccgg gcactgtagc ccacctgcgc atcgtgaacg 9000 ttgccggcgg ctcacgcagt gtgttcaact ggtcaggtgt aacgtggggt gccatcaccc 9060 ccgtggctgc cattgcggcc ggtgccactg tcctcatgac cctgtaccac aatggaagca 9120 cttggttagt caagagtgcc cagacgttct aaggaggaaa catggctaag tcagcaagca 9180 agagcctgct gaacgagctg cacgagatgc tggcgcaagt cttcattgac gacctgcgcc 9240 agtcgcggga ggagggcatt cccctccccg ccgctaacct cggtgtgatt cggcagttct 9300 tgaaggataa cgatgtgagc gcgagtatcg atgctgacga catggcgaag ctgcgtgaca 9360 ccttcgcaga cgagttggcc gagcgccgcg cactgaacaa agcccgattg cagcagacac 9420 tgcaggagga cgatatcaac gagttgcttg ggtaaggagg ttccatgatt cgagcgcaca 9480 cactagcgcg cctgcggctg ctcgtcgagc gcacgaacaa gtggaaggac aacagcaacg 9540 ccatccccgc tgaggagcga gttgatctcg ccctcatgtt cggggccaca ttcccacgtt 9600 tccgggagtt cgctgagcta gggatgaagt tcctcggctt cgagctcacg gagatgcaag 9660 gcgacattgc agactacatg caggatggcc cacgcaacag catggtggcc gctcagcgcg 9720 gtgaggccaa gagcaccctt gcggccctgt tcgctgtgtg gtcgctcatc cgggaccaga 9780 gcatgcgcat cctgattatc tcgggcggcg agaagcaggc ttcggacgta gccatcctga 9840 tcatccgcat gattgagact tggggtttac tgtgctggct ccggccagac aagacccgtg 9900 gcgaccgtac ctcgtacgag agctacgacg tgcactgcga cctgaagccg ctggacaagt 9960 ccgcctccgt ggcgtgcgtg ggcatcaccg cgcagctgca gggtaagcgg gccgacctgc 10020 tcattcccga tgacatcgag accacgaaca acggcttgac cgctaccagc cgcgaactgc 10080 tgctactgcg ctcccgcgac ttcgcggcca tcaacactca cggcaagacc ctgtacctcg 10140 gcacgccgca gaccaaggac tccatctaca agaccctcgc tcgacgggga ttcgaggtgc 10200 gtgtatggcc gggccgcgtg cccactgtgg aggaggctga gcgctacggc gacaccctcg 10260 ccccatacgt ggctgccatg ttcgagaccc atgcccgcac tgggttcggg ctggacggca 10320 cccgtggcga ggtcacagac ccgggccggt acactgagga ggacctgcag agcaaggagc 10380 tggactttgg ccccgagggc ttccagctgc agtacatgct cgacacgtac ctcgtcgatg 10440 ccatgcgcac gcgcgtgaag ctctccgatg cgattgtagc atcgttgggc accgatgctg 10500 cccctgatac cctgtactac gccgcaacgc cacagcaccg cctgcaggag ctcccagagg 10560 ctatccacgg ggaggtcatg taccgggcgg ctggcgccgg cgagctcatg ctgccgtacc 10620 agcacaagat cctgatggtg gacccggccg gttgcggtgg ggacgaagtg gccttcgccg 10680 ccggcggggc gctgaattcg tacatccacc tgttcggtgt gggcggtctg cagggcggta 10740 tggtcgagga gaactgcaat gccttgatcg atatggctga agaaatgggc atcaccgaca 10800 tcgtgcttga agcgaacatg ggccacggca ccgcatcact gctgctgatg aacgcccttg 10860 ccaagcgcaa gatcacgaac atcggggtgc gcgacatcta cgccaccacc cagaaagaac 10920 gccgcatcat tgacaccctt ggccctgtgt tccgccgaca caagttcgtc atgcacgagc 10980 gggccataga gatggacgag gaacacctgc aagcgtacag ccgcgagaag cgccgcctgt 11040 acagcctact gttccagctg cagaacatca cgtacgaccg cggctctctg gccaaggacg 11100 accgtgccga cgcagtagct cacctcgtga acgagctcaa aggatacctc gcggtggacg 11160 aggagaagga agcagagaag ttgcaagcca aagcggcccg ggagttcatt gacaacccca 11220 tgggctacac caaagggatg ggccgacgcc ccagtggcac ccgatcccgt ctacgctaaa 11280 ggagaaccaa actatggcca tgtctgttgc caacgctgcc gctgtactga acaaccagaa 11340 cgtacgcgac aaagccaccg ccgtgctgaa cgacttcgag gcgctgagcc aatcgagctc 11400 gcgtctgccc ggcaagcccc tgactggcgc tacatcggtc aagaccgatg tcgacgcact 11460 ttgcaccgcg ctggtcgctg caggagctat cacctcgttc gtatcgtaat aaggaggtgc 11520 catgagcatc acatctatca ctgaggcgga acggatcgag atccgtgacg cgtgcttgac 11580 tgcggcacaa gaaatgcagc aggtagtgca gagtggcgcc atcccacgat cggatattgc 11640 agaggcggta cagccgtctc tgcaggcggc tgcggatgcg ctggggcttg tggttgattc 11700 tacagagaat caggccctgc gcgatgacct tgctaacgag accgacccag ccctcggggc 11760 agcccttatc ggctactcag gacgtaccca agctgacaag aacgctgacg tagttagcgt 11820 gcgggacaag tccgcgaacg caggtaaacc cggcgcaacc gacgacatca cggccatccg 11880 tgatgctgtt aacggtggcg gagcgttcct accagacggc acttatccga tcagcagctt 11940 tgtgaacgtg aatgcccagc agaaggttca cttggagggc gccggtcagg gtaccgtgat 12000 taccaagaat gcggcagcta cacccactgt gctgtcatcc cgtaactcag ataatacgga 12060 ggtgcgtgat ttacggattg atctgacggg tgccaacaac gctactactg gacatgccat 12120 cgccttgatc gacacagacc atgcaaccat tcgagacatg tctatctctg gatttgctgg 12180 cactggcact ggcgttatct cgtactgtgc tgttgagacc gaccataccc agcatgtccg 12240 cattctggat agtcagttcg acggtgacgt ggcccagtcc ccaaacacca acgggttcct 12300 gatcactaat ggcctgtatt ctcgtatgag tggcttatac gccgcaggcg tgggtgctta 12360 cgctattgag tacaagaacg accagaagta ctctcacatc tcggactgcg tggcggtgaa 12420 ctccgtgaac gcgtacggat acggtcagac cactctagat gatacgggtg tgtcctactg 12480 caccgcctcg aacctaatct cgtacaactg cgggaccggg tctgtcattg gcaagggtaa 12540 gtacaacatc acctcgaacc tgttggtatc tgtagacggc ggctccaccc agaccttgaa 12600 ggctggtgtg cgtgcggatg gtacggccaa tgcgaactct tgggtgaact gcctattcgt 12660 aggcaacatc actgagcctg tacgcttcgg cgccaatacc aactttgtat cgggtacttt 12720 ccataacacc cccaccaaca tggtccagct cacatccgga gccacacgaa acgtagtaga 12780 ggtgcagcac ccggggccta cccgaaacag catctttgcg tccagcgtca tctcggatac 12840 gtccgggtcc ggccagactg gcgccaacgc caacgtcgtg ttctgcccag catcgggcga 12900 gtaccgcggt actttctccg gacggtggcg gttccagcat gccccgtccg gattggcccc 12960 ggctagctcc aaccagaaga tggtgctaga gagtaccggc gacacgttct acagcgtact 13020 gaccgatggc actggtttgg taggcctgtc ggcgcagtcc ggctcgggta ccgcgcagct 13080 cgcccacagc gccacctcag actactggca ggcctctggc cataccggcg gctggttcgt 13140 gcgcatggca aacaacctgc tgcgcttcgg tacagacgct cttgccagca tcggcaccgt 13200 caccaaccgg atgggggacg tgttctctct gcggttccgg cccggcgccg gcgcagtcat 13260 ctggacatcc ggcacgggta ctccggaagg cgccgttacc gcacctgtag ggtcgctgta 13320 cactcgcacg gatggtggtg ttggcaccac cttgtacgtc aaagaaaccg gtacaggtaa 13380 caccggctgg gtcgctaaat aaggagacaa catgaaacgt tctgtaatcg ccagcattgc 13440 actggcactc gccctaggcg gatgcagcac ccttgatgca gtgaaggcgg ttacgggcgt 13500 tgtctctcct gacaagcccg agatcactgc gcaggtgggc gcagaaaaca ccaagcaagg 13560 catcggggtg acctccaagg tggagagcac ccagaaggcc ttggaaggcg acgtgaaggg 13620 taatgctacc gtcacccaag ccaccaccac taattcaacg cagaacgcgg cgcgcgacgt 13680 tacagcgcgc accgtgactg tcaccaacac gaactccctg caggtcctcg ggatcgtggg 13740 gatgtgcctg agcgctgtgg tgctcatggc catgtacatc gtgcggcggg gccgcaagga 13800 tgaggaggaa tgaatatggg agataagctc accacccccg ctgcgtacac cgcgagtgca 13860 gtcaccggcc tgttcggcat ggtggactgg aacaccgtgg ccactgtcgg aggtctgatc 13920 ctagctatcc tcaccttcgc ggtgaactgg tactacaagc gcaagaacag caaagcgttc 13980 gaggacgcca ttcgcaaggg aggccccgtg catgagccta aagactaaga tcgtcacggc 14040 cctagcagcg agcacgctgc tggggtccgc cctgcttacc agcctgacca gccttgaggg 14100 caccgagtac accgcgtact tcgacagcgc cggcgtcctc accatctgca gcggcgacac 14160 cacggacgtc aggaagggcc agagagcctc tcctagcgag tgcaagcaaa ggctgaagga 14220 tggtattgcc cagcatgcga acgcgctccg tgggctgcct gagagcctcc cagacacggt 14280 tgtactaggt gctgtgagca acgtgttcaa catcggggag tacggcttta actcgtcctc 14340 ccagaagaag tgcttggccc ggggcgacta cgcctgtgct aaggcagcaa ccctgcgctg 14400 gcggtacatc accgtgaacg gcaggaagtt cgactgcagc acccggggta accgcgtgtg 14460 ctggggcctg tgggagcgcc gccagtggga agctcgtgcc atggggaatg agttcaagac 14520 cgtgcaggag gcactggctg ccctgccgaa gtaggagcaa gcatgtcaga gctgacattc 14580 gataacaagt tcgaaagctt caggacattc cgcggcgcgc agatgcgtac cgacatgacc 14640 atggggctgg tgaacaaacg gaagttccgg tgctacaggg agttcaccgg cgctactgtg 14700 ctgcgattta ccgctgcccg ggacttcctc cttaccgcgc aggacctgca gctggacgct 14760 gggcaagcca aagtccagat catgaccggc ggcacgcccg gcggcgtgtt cactgacatc 14820 ccaacgaagt tctgcatgaa cacgatcggt ggtgacgttg ctgggttcag cgctgtggca 14880 gccggcggta ccttcactgg tggcactgag cgcgaagtgc tccgagcgaa cgcaggtgct 14940 ggccaaggtg ctgggcgtcc gtcgcccgct ggtgcccgcc tgctcggcgc tggtacgttc 15000 tatatggtga tcactgtgac tgggactacc agcggtgtgt acagctttga gttcgaggag 15060 ctggataccg ttgctggcgt ggctgtctag gcccgcgtgg ctatccccgg gtgcgcgttc 15120 tacctgcacc cggattttat tatgctcagg cgagggccct ccctcccatc caacagcccg 15180 catgcccccg tacgggtgcg cgtgccagcg tgcgtgcgag tgcgtgccag tgcgtgcgca 15240 caccagtgcg ggagcgtgcc cgcctgtgcc cggaggtgcg cgcgtgccca gctgtgcgtg 15300 cgcgggtgcg cggctgcgcg cgtgtgtcgg cgggcgtgcg tgtgcgcgct tgccttcgcg 15360 cgttcttgcc tcgcgtgcgt gtgctggcgt gtgcgtcact gctcgcgtct gcctgcgtgc 15420 ctgtgcgttg cttgcctcgc gcgtccgtcc ggttctgact ctgctctacg gcgggccttt 15480 cctcgggatt acaccctcga cggtatcgtc tctcggccac ctattcggcg gtctcgttcc 15540 tctacctagt gcagagctat tgcggaggct ctatctcaag ggtttcagag gtgcctctgt 15600 taggctgtag aactaccgtt cgtcgggtct gggtttactt cctgtgctgg atgggccatt 15660 ctgtagccac tcccaacgag gtaagaccgg aaggcacttt agggagtaga gcctacgggc 15720 tgcgctggtt cgccagatgc gggacttcca aggcttgaca gatgcaccgc aacaccgtag 15780 tatgcgagac gcaacactgg acatggataa caagcccttt gggcggcgac catgcacggc 15840 gcagagcgag ccggggtata acaagcgcgg ctgtctggag cgatgcgtaa tggccagaac 15900 gggttgggtc cgagccaaca tctagcggcg agcccttggg atgcacggct aggcttgaca 15960 ggatgcacgg gagtcgatac actggctccc aacaaggcaa acgggccttg ggtggcgtgg 16020 atagcttact gggtgcaagc tcggtcggtg aaccgcaaag ctcgaactgt actggaatga 16080 ccagtgtgga tgctagacgg cgaagctgcg caagtggtgg ctgcctgaag gttgaagcaa 16140 caaatgccgc gatggtttgt agaagtggcc ggttgcccaa gagtcccgac tgtacataaa 16200 acggactcga tgcgtttgcc tgagcgttcc tgtggtataa caggagaaat cagacccagc 16260 ggggagagcg cggctgggag tatgctgagc tacagggtgc ctacgtcgaa agactacgcc 16320 aattgccaag gtctggcgcc tgtgtagcat cctctcaccc tgttaccggg tgactgatga 16380 tggctgacgg acagccgaaa ggagactagc atggctttcg aatcgaaagg caacgttcgg 16440 tacaacaagc tcgccaagcg cgaagcgaag gtacaggaac tgcgcaacga aggcacaccg 16500 gaatcccgta agcaactgaa ggctatgctg gactggcgtc ccgagtggga gaacgctggt 16560 catcgtcgtg cggtcactcc gaacggcggc atgatgcaca atccaaaggg taagcgtcac 16620 gtcaagggca gctctgccaa gtgtgaccgt atcaaccgtg gccaccgggc caaactcaat 16680 caaggcaaag gtatctaatc atgaaactct tcgaaggcgc agccgcaatc aacaaagaaa 16740 tcggcctgat caagaccgcc ggcaagaagc tcgacgaccg tattcaggtc gccggtgtga 16800 gcgtgatcgc ccactgcggc cagcacaacg atgtcactgt ggtgaacaac ctgttcctcg 16860 ctatgccagc gggtgctcgc aagaaagcgc tggccgagtg gctgttgaag ttcgggaacg 16920 tggccgcgaa caccgacaag gcgtccagca agactgcgcc cttcgtgtac gccaaggaca 16980 agcaggcgaa cgtcgaaggc gccattgccg agccgtggta cgacttcgca ccagagccgg 17040 ctgtggtcac catgttcgac ttccaagcaa tgctggcgag cctgctcaag aaagccgaga 17100 aagccgagca acagggcctg aagatcgaag gcgccgaggc gctggcggcg gtgcgcaagc 17160 tcgccaaggt gaagtaagac catggccggg ttcgtgctcg gcctcgtggt gggcttaggc 17220 atcggctgtg ctggtgcctt ctgcctgctg gcgtggttaa tcatcgggag acgctgatat 17280 gcctattctc ggcttactgt tcgctttgca gggcatgtac cgggccttta ctccaccggt 17340 gcgcaaacaa cctgaggtac gacatcatgt ggcaagcact gtgctacacc ggtaatgggt 17400 acgatgtgtg ctggagacca ggcaacatcg ccacgaacat ggtccggcag agtgacatat 17460 gctggccttc tgccccttac actcctgtaa ggctctgccg agccgtgtga ggtgcgtcat 17520 gctcaggata ataggttggc ttgtgttctg cggtggcgtc gttctggcgg cctctggtgc 17580 gctcacaggg tgtgcctcag ccgttgcctc gggcgggatc actcagcaga acggtacttc 17640 acattggcag gagtacaatc aggccgtgca agacgagtgc gacaagcagg tggtgaagat 17700 cgccaccgag gccggcgtca ccgccgacaa catcaaccgc ccggatattc agcgggcatt 17760 gcgtgaactg cgctggcaat gcgcccatca acaagggctg acatcatgag tataccggtg 17820 cgtactacct tcattcaaag gtatgctgct gaaatcaagg ccacgtacaa attggatgtg 17880 cagttacagg tacgtcgtat ttcaagcggg cagattatgt ccccaaatct gagccgggca 17940 ttgactctta cccttgcagg tgggatacag ggatcagcgt cttataacag ggtagtatgg 18000 ctgctaggta cgggtgtact tccagataag ccagttgtaa agactggact gggttatgca 18060 tgtaacggtg taatacttat gcccgagtgt agcggagaac ctgcggcaca aagggcagca 18120 ggtggggcct tgcgcttgga tgaggccagt ctactaaagc agtttgatgc cgtagtatcg 18180 gcccttaatg aacttggcat cgacccagtt cggctagtgc gccgagttac cacagaggtg 18240 cccttatgat cccccacgca ttcagcattg cagatatgcc ggcaatgttg cggctgctag 18300 cagatgagct agaggaatct gccgacgcat tggcggtagt agtgtgtatt aataggatgg 18360 gcggtctaga cctgcgcggt tacggtcggg ctgactggcc agacgcagta tgtcttctta 18420 agcaagctat aggtggccta tgatcaagct cccaaagccg cgcgcccgtt ttatcgacgg 18480 gcattggcat gtgtactcca ctggtacgat cacctgctcg ggctggacca ttggccaagc 18540 cctactggcg tgggtgcatc ggaactggac ccagagcagg atcatgcgcc catgaaaccg 18600 atcatccagt ggatgccggc ggcgcataac atctacatca ctaagagcgg cggtatgcaa 18660 tcaggttatt gggcttgcag gcaccgcaga ggtttagcct ataaatcggg cgtgggccgt 18720 acaccgcgtg aggcgtacga gatgtgggca cagatcaacg aggtgcggcc atgattgcaa 18780 cagcaaatgg tgggcgcgct gccgtaggca ccgagctcga tgagtcggtg cccgctgctg 18840 agcgcgacgc aatcatcgaa cgtgagcaac gccttgcagc gtggcgggca agcctgccga 18900 aacgtaagcc ccgggcggat gagctgcccg ggccgtgtga aacctacaac tgtgccggcc 18960 taggctggac ttgggagggt aagtgagcga ctgcattcca accaaacgcg ccaagaattc 19020 acagggctat ggctatgtgt ctcgcaatgg tcgaatggtg ggcgagcatc gcgttgttta 19080 ctgtgactac aacggcgtga cgttagattc aatcgctggc ctagtagtac ggcacgcatg 19140 cgataacaga tggtgcatca accccaaaca cctgatgctt ggcagccagt ccgacaacat 19200 gaaagacatg gcagagcgcg ggcgtacctc tgttcgcaga ggcgaggata acccaaatac 19260 aaccctaacg tgggatgacg ttagacggat tcgaactttg cgtgacgacg gcgctaagca 19320 agtcgacctc gcccgcaggt tcggtgtatc acaaggaacc ataagtgcaa tctgctcgca 19380 caagctatgg cgcgagccct aaacctgcag cccattaaag gacacgacaa atgagcctga 19440 atttcgcaat catgccagcg gatgccggcg cctcccaaga ggccaccgaa acccgcgaca 19500 agctggccaa gattatcgag agcgttggcg ttctgagtga cgcggcccct gagccgctga 19560 gcaaggaccg catccgcgac ctgcgcaagt ccggtgccga gttatttggt atgttctacg 19620 acggcgagta cgcccagacc aactacagcc cgaccataac cactcaggcc cggctcatca 19680 atgggctgac cgcaggcatg ttcaagatgg ctgcccgcga atcacgcagc accagctcgc 19740 gtgacctgac ttacatgtgg gaaggtatcg ttaaggcctt caatgcggct gtgcatgcgt 19800 cgaagctgaa gatggacgac gagttcaagg ccaccttggg cttggcgttc ttcaaggact 19860 accacggtgg gcacgtgcat gtgtggctgg agcgtaaagg cctgttgcca cggccagtgc 19920 agtattggcc gttccaagag aacatcgagg agttcaggct gaacctgttc gtgcactacc 19980 gcctcggcaa tgacggcgca gcggacaagg ccacccgaga gaccttgaat caggtactga 20040 aggaccagtt cgctgtggat tggaccggta attcgtggcg cgtagagctg ctccattgcc 20100 gccccgtcat gatggtgtac ttcgacaccg atggtccatg ggctgctgga ttcgctagcg 20160 ccgatgacgc ttgccgcggc cgtttcaccc gctgcccgct gggctcgtgg ttgagtgcgc 20220 gtggtgctga tgatgagacc gtcaagcagg aagtggagcg catcaaggcg atgaattcca 20280 ctggcgactt cgaactgtac ccgaatgact gcttgttcgg tgatttgtac gaggcaggtg 20340 cgagcgacgg cgtgagcagc tgcatggcct gctcccgtga tgagttcaac ctgtgggact 20400 accaccaccc aaccgatgcg tacagctctg cgtaccatgg tagcggtgac aacgggctgg 20460 tgctcgtggt cgagcgcaag aacggtaacg tggccggccg tggcatcctg aacgtgaaca 20520 ccaagaagta cgtgcgctgg tacggtgccg tgaccggtga tcgtgcactg acccgggcag 20580 gcatgaggcg tacatcgtcg gccttgaagg gctcgtggct agctatgctg cggcatccaa 20640 ccatggaaag ccgcatagtt gcgccgtacg tggacggtga ttaccaaggc gcccgtgtgt 20700 cccggtctga acggcgtgtg tacttcgtag acgatgccgg cgatgccctg tgcgaaactg 20760 gcgggtacta cgacctcgac tctgtgtact gcgtggacat cggtgatcat aacgaccgct 20820 gcgacagcac gtaccagtgc gagcacaaca actgggtaag cgacggcttg gaggacgact 20880 ggcgctgccc agtcattgct gaatggtgcc acgaagacga acgcgtcgag atcatgctcg 20940 atggcgaaat ggtgtacgtg tgcaaccggg ttggcgagca acgcacctcc cagtacctga 21000 acgtcatcaa gagcccagaa gacgacccgg acgctgtgaa ctacaccggt cgcggccgct 21060 gggctactgt gcaggataac accccgtaca ctccgccgcc acctgtacag cgcttcagct 21120 gggatgaact gccaatcgcg caggccgcag ccccggcgcc tgtacttgca gccgatattg 21180 cgcagcagct gccagccgca atgtccatct atgcacaaat gggctgcacc tgtactgact 21240 gccgcatcga acgtggagag cttctgtgag caattcaatc gaggttcccg aggtactgaa 21300 ggccctgctg accacccgcc ggccatacgg cggtgagggc gaacgcgccg cgaggctgct 21360 cgttcttgac acggtgttcg aagctgggta cgtcccgacc gtagatcagc atgggaacgt 21420 gcaggtactg gtggggaatc gcccggacct cgtgttcacg gcgcaccttg acacagtgca 21480 ccgtgccgat ggcaagcagc tgctgtggct gttcgaggac accgaggaga tcatggcaga 21540 cgggctggac aacaagccca gcgtgctcgg tgctgacgat gccagcgggg tgtacctgct 21600 cacggagatg atcaaggcgg gcaagcccgg gcggtacctg ttcttcgtgg gtgaggaatg 21660 cggcggcgtc ggatcatcgg cgttcgtgca agaccaccca gacttcagcg ccctgatggt 21720 cgtgtcgttc gaccgccggg cacgaaggac atcatcacgc accaagctgg gtactgcaca 21780 gccagtacga cgtttgcgct ggcgctggcc gatgcgctga acaagcagaa gcccggcgtg 21840 ttccagtacc tgccatgcga cggcgggatg tacaccgact cgaaagagtt cgcggagctc 21900 gtgccagaat gcacgaacat cgcggtgggg tacgaccacg agcacacggt gaacgaggtg 21960 caggacctgc ggcatctgct gaacctgcgt gacgctgtaa tcggggtgga ttggacggcc 22020 ttacccatag cccgggtacc cgaacctgac accccatggt ggcccacatg gaacaagaca 22080 ctgggccgtg actggcacaa cgatattccc gacgacccac tgcatcagcc tgtgcgaggc 22140 gcagtggcca tccgcaacct gctcaaagag ttccgagatc acgcttggaa caacggcatc 22200 gacgacttcg acgagctgga caacttcctt caaaagatag aggacatgct atgaccatcc 22260 tgacccgtat cgcatcaggc ctgctgttca tcgaccacca caaggccgcg ctcacctctc 22320 gcctgctcag cggtgccgcc cgactgaaca ccctgcatgc cgaatccttg gccaacaaag 22380 ccggccgcaa gctcgtcacc ggcgaggcta aggcccgccg actggccgag tcccgtcgtg 22440 agcaggcgca tcgtgcgctg gcgtcccaac tgcggagcat ccaggaggac ctgcagaccg 22500 atctcgccaa ggtgtacatc gagcgcgaca aggccgctca gacagcggca tcgtacgagg 22560 ctgtgggtta ccattgccat gcctcggcat gcgtgcgaga cgagcgcgca gcggtcctga 22620 agggcatccg cgaggagctg cgcagcaatg gctaacaaac tcgtcgagtt gctcgaactg 22680 caggttcgcc taccactgtg gctgttcatc gccggggcaa gcagcctcgg ttatgacgcc 22740 atcaacgcac tgggagtatt gggatgaaca acaaggtatc actggccgca tccgtaatgt 22800 gcggcctgac gttgggtgcc ctcatcgttg cggctatcac gggcggctta tgatcccccg 22860 ggcggagtgg ctgaccgcag cccaagccct cccgattggt ggcaagcgca ggatcaagca 22920 cggcttcgag gccaccgccg ccatggatgt gtggaacaac gaagatagct ggtcctgcta 22980 ctgccatcgc tgccacgagg gcgggcgagt gccgaaggaa catcagcggg tgcggcgcgt 23040 catggtcgag cccgaccgcg tagcgcctgt acctgcaacg gtaattcgct tggcagatgc 23100 gagccgctgg gaacagcagc ggatttggga attactgtgc cgcaagggtt gcccgccggg 23160 cgtaatcccc gaggaggttt tatggtacga aagatcagtg gatcgtctga tgctgctatc 23220 agacgacgtg gcgctcggga gggcgctgga cggcaggcgg atgccgaagt ggttaccgta 23280 tggggcgtgg cggggcaagc cgatgatgtg ggcgactcgt accagtgccg aggcaacgga 23340 acctgcggac tctgcgccgc ttgggtcgct gatcctgacc gaggacgcgc tctctgcgta 23400 caaagtggcc aaggccatcg acacctatgc gcccagaagc tcgctggacg tatgcgcgac 23460 cctcgggact agcatcaccg accggttcct accgtacaca ctgggccggc ctgctgtgct 23520 gtgtatgtac gacggcgatg ccgccgggct gcgtgggtac agggccatgc gtcaacgtct 23580 ggctgtgtgg cagcagcccg tgttggacct acgcccagag cagggcgacc cgaagaacaa 23640 cgacctagca tctatagcgg aggcactgag taagtggatt tgaatatact cgccgcccta 23700 cgagagcgga ataagtttcg gcagttacgc ggagctgtgc ccgactctct gctgggccaa 23760 gaaaccgtga gcatgctagc gtggtacgct gcgtacttca acgcattccc cgacaaggac 23820 tacgtggata ccgacgtact gcgctcgctg ttcaccttga aggtgggcag caccgccacg 23880 ccagagcagc tggccgtgat gcgagcgctc atgaacaaac tcgacgagcc agtcgatccc 23940 gaggtaatcg acggcatcac cagtcagctg tacgagcgcg acttcgccgg ccgtgccgcc 24000 gccctgctga accagtacga gaacggtggc gaggtggaca tcacgtacga gctgaatcgc 24060 atggccagcg agaacatgcg ccggctcagt gcggcagccc cgagttcgta cattgacacc 24120 ccgatcgagg atatcctcgc agacttcggg caggaccgcg ggctgaagtt gcctaccaca 24180 gccctgaagg agaccattgc aggccttcag ggcggggaca gtgtggctat tgcggggcgc 24240 ccggacaagg gcaagacgag cttcctagcg gcctctctga ctggtttcgc taagcagctg 24300 aaggggctcg gtctggacgg acgcccgctg ttgtggctga acaacgaggg ctcgggcaag 24360 cggatcatcc cgcgaatcta ccaagcggcg ctgaacatca cgttcgagca gatgctgcag 24420 atgtcgaacg ccggcacgct gcgcgatgcg taccagaagg cggtggatgg gcaggagatt 24480 cgcgtgaagg attgccacgg cagcacgctc ggacagcttg agcagatcat cgaagagatg 24540 aacccgtgcg ctgtggtatt cgacatgctc gggaacttcc gcatgcctag cacgggcggc 24600 gggaacaaga ccgacgcact ggagcagatg tggcaggagg tgcgggagat ggctgtgcgc 24660 cacgacttcg tggccatgcc aaccatccag atcagtgccg acggcgacaa catgctgttc 24720 ccgccgtaca gcgcactcaa ggatagcaag accggcgtgc agggcgcgtg cgatgtgatc 24780 ctgatgatgg gcgcattcaa cagtgtggac ttggacaacg tgcggggatt cagcacgccc 24840 aagaacaagc gccagatgcc gggcaaacca agcaacgtac agagtcaggt gttcttcgat 24900 gcaccgactt gccaattcct agatggagaa tagcatgacc acgaccgctc agaagaaacc 24960 cgcagccaag aagccgacca ccaaggccac tgcagcggct gcaaagccag tgatcgatga 25020 gaacgctctg ctggcccagt tcgctgcagc attgctgcgt gacggcacgg gccgcattga 25080 tgccagcgat gtggccctgc gccagcgagt ggtggacgcg ttcaaccgcg cccgcgtcat 25140 ggtggacgag ttcaaaattc aaatcggagc ctgatcatgg cacaagtcct gctggatgtt 25200 catcccggcg cattcatcag cgccctacgc aacaacatcg accgccgcga acaaatccgc 25260 agcgccaccc acgacaccgt gaccctgtgc aagaacattg acagggcgct gtccgtggcg 25320 gcagtggtgg ctgccgaggg caacgacaag cgtggtgggt tcctcgttct ggatggagag 25380 ttgcaaggcc tgcacagtat cgacaagggc aatggcgact ggttgatgcg cgccgccgtg 25440 gggcagtacg gtgcgaaccg tctggacacc ttcgacatcc cgaacctcgt ggccttgtac 25500 acccgccacg gattccgtga ggtgctccgt gagccgaact tgaacggcgt aggcccagat 25560 gttatctgga tgaggagaga ctgatgggcg ccaagagaga gcgcgtgctg ccagtggact 25620 gccaccgacc ggtgaactgg aacgaggcag cagtgcagcg cgtcattgat aacgtcgggt 25680 atgtgtacgc ccagaccaag aaggatggca tccgattcca tgccatgatc gatgctaacg 25740 atcgggtgcg tgtgctgacc cgtgagggca tcgagatact gtcccttgac ctgcacaagg 25800 aacgcctccg tgggctcctg caagccctgc ccaagcagtt tgcgctggat ggtgaggcgg 25860 tagtaccggg tatcccgttc gaagaagcca gcggcatcct acggcgccac aaggccatcc 25920 cgctggagga ctccgtagag ttctgggtat gggacgcgct gcccgttacg gaactgttgg 25980 ggtacgaggc cgggactgca acactggacg agcgtatcga cacgctggtg caggcattcg 26040 gcagcacgcc ggagccgcag gcgcagatcg tgcacatgga accggtgagc agcatggagg 26100 aactgtacct gctgttcgat gcccaacgtg agctgggcga ggagggtctg gtggtcaagg 26160 acccgagcct tgcaccgcgc aatggcaagg tcagcggcgc atggaagctc aagccgagcg 26220 acacctgtga cggccgcatc accggtgtgc tctggggtac gcccgggctg ggcaatgccg 26280 gcaagatcat cggcttcacc gttgaactgg agaacgggaa cctatgcgat gtggatggcc 26340 ttacccaagc agccatggct gaatacaccg ttcaagttga gaactgggcg cactgtcaat 26400 ccccaccaga atacgtatcg ccgcacccat tcgttgggcg gtacgtagag gtaagctaca 26460 tggagatgac cgccaagggc agcctgcgac acccgaagtt caagaccttc cgggatctgg 26520 attattgccc cggatggaaa tgttaaatgc atgcatagac catgggcgcg gtggtgataa 26580 gcaaggttac gcgcaaactc acggcacccg tttgcatcga aaggtgtatg ccgccaagat 26640 gggactgact cttgagcaaa tcaagggcgt cacagtacgg cacacttgtg acaatacgcg 26700 gtgcattaac ccagaccatt taattggcgg caccatgtta gataatgcca gagaccgtgt 26760 tgagcgagga cgcaacgcca agacacagcc atcccgttgg aagcttacag aagagcagtg 26820 ccgagctatc ctacgcagat tcagcccacg gcgtgttggg ctgactgcac ctgatggtat 26880 aacccaaatc gcccgcgact acaacgtcga tcgtaatgtc attcgacaag tgctgaacct 26940 tacctacccg gggtggaaat catgaggcgt attgcaggct tcttctggta tcgcggcgcg 27000 attgtacgca tccaatcata caatccgatt ccgccgtatg agtttacgga cagtacggcg 27060 aaccaacgac gcgcccagtt ccattgtttt gggctgttgt tcaatcctaa agccttgtgg 27120 gtcggcgccc actacggcga gcatcacaag cgttggtgca tcaacattct gccatgcctg 27180 acagtgtggt ggaccaagcc aggaggctac ctcccgtaat agaatgacac tctgtgccca 27240 gaatgggaga ggattcgatg tacaagatat tcatcactga cttagagact gagaacaagc 27300 cttggtacgg tcagatagca tcaccattca accctgagaa ctacattgta gcccctgggt 27360 ggcgagtgga caccgtgcat gacgatggct ctgtcgaggt aggtgagacg aagtggaggt 27420 acttcgcatc cgctgaggag gcagacgccg gggccgactg gttcgacgag ctggaggggt 27480 gcgccatcat ggtggcacac aacagcatgt tcgagcagaa gtggttcctg agcaagtacc 27540 gggagcgctt cgaggcgttc ctgaaggccg gcggtaggct agcatgtacc caaatggctg 27600 agtacctaat cacggctcag caggaactgt acccatcctt ggacgagact gccgtgaagt 27660 acggcggtac gcacaaggta gacggggtaa agattctgtg ggagcagggc tacctcacca 27720 gccagatcga caaggacttg ctgatcgaat acctcgccgg cccgtccggc gatatcgaca 27780 acactgcgct cacgttctac ggccagcagg agaagctggc agagatgggc atgaccgaca 27840 tgtactggga gcgctgcgac tctaagctgg cattcggtta ctgtgagtgg ttcggcctgc 27900 acgttgatcc agaggtagct gagcgcaacc gcgcagcaca ggaggcggag atcgcagaga 27960 tgcgggcgaa gctgaacgag ctgctgccca aggacatgcc cgactggatc gagttcagtt 28020 ggacgagccg gtacgatgtg tcggccctga tctatggggg cccggtgaag ggtcgcttca 28080 aggggccgta cgacccgccc cagtacgtca aggccgacta cgtgaagact acggacggtg 28140 caaccttcct gttggaggag atggatacgg tcacgctgga cgccggcgcg gactgggagc 28200 ggtacaagtc gggcaagaac aagggtcagg tcaaggtgtt ccgcgaggac accgacgagg 28260 agaagctgaa gtggcacgag ggttcgtacc ttatgcccgg gctcgtagac ctgaagtccc 28320 tgcccgatgt gatcagcagc aagtaccttg gcaagcgcgc cgagttccgc ggtgcccaga 28380 ccctgtgcga tggcgtgacc ccggtataca gcaccgctga ggaagcgctg aagggcttga 28440 agaactacgt gcctgaggtc aagatcatgg ttgatctggc caaactggag aaggataccg 28500 gtacgtacta tctgcgcacg gagtacgatg ctgatgggaa tgtcaaggaa gtcaagggca 28560 tgctccagta cgttcagaag gaggacaaca tcgtccacca cagcctgaac accgaggcga 28620 ccgtgaccac ccggctcagc agctcccgcc cgaacctgca gaacctgcca cgagcagacg 28680 aggacgcaga cggggaggcg aagtcccgcg tgaaggaaat gttcaccagc cggttcggcc 28740 cgcagggcag gatcatcgag gtggactaca gtgcgctgga agtcgtgatg ctgtgcgcca 28800 tgaccaagga catggacctg ctgaagctcc tgcaggctgg taccgacatg cactgctacc 28860 gtttggcgtt ccagctgggc gagccatacg aggaggtgct ggccaaggtc aaggatgaga 28920 gtcaccctga gcacgggaag tacaaggccc tgcgctcgaa catcaagccg ctgagctttg 28980 ctgaccagta cggcgcatca gcagcggggc tggcgttcaa cactggctgc tctctggagt 29040 tcgctgagca gttccaagag aacgaggcga agctgttccc gatcagccgt gggtaccgtc 29100 aggtgatcat cgacgaggtg aaccgcaccg gcagcctgcc gggcagcatc caccgcgaga 29160 tgtacgacaa cggccagtgg ggcgtgtacc gtcgcgggta cttcaccggg ccgagcacca 29220 ctcggtacag cttccgccag cagcgcactt gggacaagga gctgcggcag gaggtgatgg 29280 cgtacaagcc aacccagatg gcgaactacc cgttccaagg cgaggccggg ttcatgatgt 29340 cgtccagcat gggccgcatc tgccgctggc tgatctccgt ggactggttt gacaatcagg 29400 tatgcttgat caacaacgtg cacgacgcag cgtatctcga cgcagcaaac gaggaggtag 29460 gccgggtggc agcgcttggc accaagagaa tcatggagga cgcaccacgc tatctcaccg 29520 agatgtggcc ggcgttcgac atggccgatg tacccttccc ggcggctgct gagatgggcg 29580 cgaacatgat ggagaaatac cacgtcgagt gaggcgtggt ctgtacttgc agcgtaatat 29640 cccaatcccg aaacgaggaa acatgaatgt ctcttgatat cctgaacagc ctgatcgacg 29700 aagccatcga actccagtca gttgacatga ccgaggaagg cactggcggc ggtggcctga 29760 tgccagaagg ctatgccatg gcgcggatgt gcacctacat cgagctgggc cagcagccgc 29820 aggaattcca aggcaaggcc aaggcgccgg ccgacgagtt caaggtcggc ttcaagctgt 29880 acggcggcga ggacgactgc tacgaaggcc gcttcatcag caccttcgat ctggccctga 29940 gcaacaacgt gaagtccggc gccaagaagc tgttcgacaa gctgaactgg aagggtgacc 30000 tgcgccacgt ggcccaagcc ctcggccgtc cgttcttggt accaatcact gtggtgacca 30060 acgcctctac caagaagcag tcgaaccgca tcgacttggg tggcatcctg ccgccgatcg 30120 acccggtgag caagaagaac tacccgattc ctgatctgga cccggccgac ctgaagtatt 30180 tcttcttcaa caagccgacc aaggagactt gggactccct gttcgtagat ggcacttggg 30240 acgacggcaa gtccaagaac aaggtgcagg agaagatcat gtcggccctg aacttcccgg 30300 gctccgatct ggagcagctg atcagcggcg ttgtgctgcc tgacttggcc ccggatgagc 30360 cggccgacga gccagagccg gagccagtca agactgcgcc caaggccgct gctccgaaga 30420 agcctgtagt gccgaagatg ccagccatgc cgaaactgcc tgtacccgac gcggagtaag 30480 ctatgagcga cctgaccagc ctgctggaag gtctgccatc ccagttcgag cgttccgtct 30540 ccggacggac gctcattctg gatgctgact tccccgcgta tgaggcctcg gccacggtga 30600 agaagttgcc caccgccctg acccgcttcc agacgctggt ggaaaccgag gtgttcctga 30660 ccagctccat ggatgtgcag gtgcacctga ccccgcgtgg ctgcacgaag tgccggcgat 30720 acgactaccc cacggccaag ccttaccaag gcaaccgtga aggcaaggct aagccgccgc 30780 tgctggagcc gctgcgcatg gccatcccga gccacccttg gcctgagcac tggtctacgt 30840 tcgcatggcg tgaccgcgag gccgacgacg gcatgatgat gcacagcttc gccctcgggg 30900 caaacggact ggtctgctcc ggcgacaagg acctgtgcct gaccccacat ccgtactgga 30960 tcatcgacga gggccgggtg gacatcatca aagaccggtt cggctggatc aagcgcaagg 31020 agtgggacaa ggactggaag atcgtcggcc acggcacgaa gttcttctgg tgccagatgc 31080 tgatgggtga cccagctgac aacgtgcagg gtatcctgcg ctggtacgga aagaagtgcg 31140 ggaagcaggg tgcatgggag atcatgcacg agatcgacaa cgaggatgag gcggccaacc 31200 ttgtgctgca ggcgtatgcc gacgcagggc aggacttctt ggccgaggcc cagtgcttgt 31260 ggctgcgccg gtcgctggac gactgcgcat tcaagtacct gagtgagctg gaccttaagc 31320 cccaactgcg cgagtggatg ggcatgctgc acgactacca cggccaatac ttggccgcta 31380 ttgacgaaca gaaggaattc gaacgtggcg aagatcaatc aagcgaaggc tcgggaactg 31440 aagcatcagg gaaccccagc ggcatccgtc tcccgtggga agactagtct gatcggtgac 31500 ctccgggccg ccgcgctgga cgcgtacttc gccggcaacc accgcgagct ggagaacatc 31560 atggcccgcc tgaagaagga gagcccatac cacagccgcg aagtgtacga agaaatcaac 31620 gagctgatca gctacggaga gtgacatggg caccgtaacc ctactgccac cgagcggccc 31680 gccacctgtg aatgaacagg tggtagaaat gttcgaagac ttgttggccc gcgctaaagc 31740 gggagagatt gatgcagccg cagtggcgtt tatcagcggg cgtgtgaaca tcctgacgac 31800 gtttactgat aacgatgagg atgtattctc tgcgcttatc ggcgcggcca ccgtcctgac 31860 tacccgcatg gccatgggag actagcatga ccgttattca gtacttggca gcgttcgctg 31920 acctactcat tacccgcgcc agtaaagaag gcattgcgcc ctgcactgtg gtgtaccata 31980 ccaacgatga cggcggtggc ggttgcgcca tcaccggtcc tgccggcggc tgcatcacct 32040 gcgatacaaa gtacccgctg gatggcgtgc tcaaggacga ctacgtgccc gagctgacta 32100 cagaaatttg gcagctattc ctacgacaag agaagccgca ccgcttccca ccagtaccgg 32160 agacgctgca atgagcggtt acagcgggca cactatgagc aacgggcgac gggtgcggac 32220 tgacccgcgg ccgcatcgta agcaggcatc gcgcctacgt caggcgcaac gaatggcgga 32280 acaggaggat gccctacgtg accccacgaa agctgactag atcccaactg cggcccctgg 32340 gcatgcggat gctgcaagag caaggtggcg tgtgcgccct gtgtggcaag ccgatcgacc 32400 tgagcatcaa gggtgagatg gtcattgacc acgaccacga gacaggcctt gtccgcggtg 32460 ccctgcaccg ctcctgcaac gcagccgagg gcaaggtggc caacgccgct ggacgctggg 32520 gtgccaagag catgtcgtac acggcgatcc tgccgtggct gacgaacctg ctggcgtacc 32580 tgcagaagac cccacagcct tacatctacc ccacacacaa gacggacgag gagaagcgcc 32640 ttgaggctaa cgccaaggcc cgccgcgccc gcgccaccag acaggcacgc caagtcgtgc 32700 gcaaatccgt aatcaagaag gactgacaat gcgcctcctg aaacttctga cgaaagacca 32760 gcatatcgaa gtgctgaccc gccatgcctg caacgtagag gcagcgcagg tatacagcat 32820 cgccatgggc cagccggtga cccgccagaa cgtggcgtac tggcgccgca tcttcatcga 32880 cgagggcacc aaggccaagg ctgaccgact gctggccgcc ggccgagcac tgcgcaaagc 32940 tgagccgggc gacgacgtag gcgacatctc gttcgtgccg aaggtggccc accgcatcct 33000 cgtgatcgga gacctgcatg agccgtacgt ccatcctgat gcctacgact tcctcgaaca 33060 cctgaaggcc gagtacaatc cggacctcgc tgtgcagatc ggggacgaga cagacggcca 33120 tgccctgagc ttccatgagt cagacccgaa cctcgacagt gccggcgtgg agctggagaa 33180 ggccaaggag ggcctggccc gtctgtacga cctgttcccg cagatgctgg tgtgccactc 33240 gaaccacggc tcgctggtgt accgccgcgc gaagtacagc ggcatcccaa tccagatgat 33300 caagtcgtac cgcgagatcc tgttccccgg gcacagcgcc tgtggctggc actgggcctt 33360 caactggaag atcgagaccc cgctgggtcc agtcctgttc cgtcaccaga ccaccggtga 33420 tgttatggac gccgccgccg ctgagggctg caacctcgtt gtgggccacg agcacggtaa 33480 gttcggctcc cgctggggtg ccaccagcat gcgcctgttc tatggtgcat acaccggctg 33540 cctgatcgac aaggacagca tggccttcgc gtacggcaag acgtacccgc gcaagccaat 33600 cctcggtgcc ctcctgatta tcgagggcgt tcctgttaac gtgccgatgc tgctcgacag 33660 cgacggccgc tgggtagggc cacagcaatg agtcatgatg ttaatgcgaa gtgtgtgggt 33720 tgcggaggct tcttctactc gcaagagccc tttggggatg tgcggtgcga gtgtggcacc 33780 gtgaactttg ccaacaaggc catccaacgt tatcgagccg ccaccaagga ggcacctgtg 33840 actgtacctg ctcaagcaac gcccggcaac gccggcggta tgaagttcga cgcgggcaag 33900 ccccgctggt ccctgctgat gggaggtatg cccggcgccc tgactggggt ggctgaggtg 33960 ctcacctttg gggccaagaa atacgcggcc cacagctgga agacggtgcc cgacaaccgc 34020 gagcgttacc gcgatgcgct gtatcgccac ctgaatgcgc tggaatcggg tgagctggta 34080 gaccccgaaa gcggcctatc acactgggac cacatctgct gcaatgcgct gttcctgtct 34140 caactggcta aggacgcaga caagtgatcg ccgccctagc ggtgttcacc gcagcgtacg 34200 ccaccgtaat gctgctgggg ctgcagtcca agctcatgcg cgacaacaac tggcgcctga 34260 gcttcttcac cagctggttc atcacgttcg cccaaacggg cagcacgtac gccatcgccc 34320 acaacgccct gcccatcgag tggttcctgt tcttcagcgg cctcggcggc agcaccggca 34380 ttgtgtcggc acacttcgtt tacgattggt acgacaagag gtttaaacga taatggaaga 34440 cacctacgca tttgttgaag atatgctggc cgccttggag tcggattgca atttcctaca 34500 gggattatgg ttggatcaac gtattccaca ggatatacgc gatgctatta tggcacgcat 34560 tgaaacccaa gaccgccgca atgaagactt cacggcgatg ctggagagcg cataatgctt 34620 ccacaactcc aagcagcagt acacaaaggc ctaagcgaca gcctgcacgc agcgatcgac 34680 acaggaaacc aagaagaagt atatggggcg gttgataccg ccgaagaagc ctttgcactt 34740 ggccagattg atgccatcca gctgggtgac ctgctggatg acttccgcat ctgggaggag 34800 agtttgctgt gatgttcgac cttgataacg tggactacgt aggccccatc tgcgccctga 34860 ttgatgatgg gaatttcgat gccgcccgcg ccctgttgca agaagtacct gccaagctcc 34920 gcgagtccgt acgctggact atcgcatcca tgaagaacat cgtcctctga ggtaggcatg 34980 gacctgattc aacggcaaat caacatcgag caagagtaca ccgcccaagg cattgcgctg 35040 gcaatcaaga agtgggagac ggacattgcc gagggccgta tcgcggacac tggtgtgggt 35100 aagcgcatgg tgatacgtgc gctagccggt gttcgctccg aactggatag catctgtaat 35160 gccggtacac gcggcgtggg cggccgctat cgcagcctgc tacgtgatat cgggctggac 35220 attgcagcgg tcatcggcct gcgcgccggc ctcggcttag tcaccggcca gcgtgcccgt 35280 aacggcgagc cccttgtgca ggacctcctt agcgaagtag gcgcacacct agagatcgag 35340 catatggcgg tcaagctgaa ggcagctgcc ccagcatacc taggaagggt cttggacagc 35400 ctgcaggagg cccgtacgcg cagttctaac cacagacgac gtaccctaca ggctaccgcc 35460 gataacgtct ctgtgggctc tgagaacgtc acatggagca ccggggagcg agagggtgcc 35520 gctaagctgg tgctggaggc gctggttaat ggcggggtgc tggacatgta ccacatcccc 35580 aaaagccgtg gccagagctg ggtggccctc aaagccagcg atgccattgc tgcgcacgta 35640 gatcgcatgg gcggtgcggt gcgggcattc atgctgtttc cgccaatgct tgtgccgccg 35700 aagccgcata cccgcgacac actgttctct ggggcgtcat acctcacaga gggtatgtca 35760 atcaactcgg caagtattcg agtacgctcc cggcgtgcag accatcgaca gtggatcaag 35820 gacaatgtaa gtgatacggt cctaactgcc gcgaacgtgg ccgcatctca gccgtactgc 35880 atcgatgtgg agacggcctc gatcctgcgc gacctgttct cccaaggtgt ttacaacggc 35940 atcgcgggca taccgtcacg tgatcccatc cagccgccgg cgtatccgct caaggagaac 36000 tgggaccgcg aggaccctgc tctacaggag gagcacgctg cgtggaaggt gctggctcgg 36060 caggcctacg ccgacgagct ggcccggaag tcgcacgttg tgtcgttctt gcagaccctg 36120 aagtatttga aggagtacgc aggagatacc ctgtatttcc caacatactt cgactggcgc 36180 ggccgtctgt acttccgcag ccggatcaac ccgcaaggca cagactttgt gaaggctgtg 36240 atccagtttg cgaacaagaa gccgctgggt aaacgtgggc tgtactggct gaaggtgcat 36300 gtggccacct gctacggctt cgacaagaag ctgccagact gccgggcggt gtgggtggac 36360 gagcacatgg cgcagatcaa ggacgctgtg gcccgacaca tcgactccga cttcttccgt 36420 gaggccgaca gcccgtggtg cttcttcgtg gccgcacggg agatgctgcg cgccatcgaa 36480 tccggcgacc ccgagtcgtt cgagacaggc gtgcccgtcg ccatggatgc tacttgcagc 36540 ggtatgcagc acctatcggc agtgctccgc gatcccgtgg gtggtatgtt cacgaacctg 36600 ctgttcaata acgggaccga gaaggaagac atctacgccg gtgttgcagc aattgctata 36660 tcccgactgc aggctgaccg ggacaatcct gagcaggccg cgtactggct gacccacggg 36720 gtgccccgca gcatggcaaa gcgcccagta atgacgtacg tgtacggcgg caccctgatg 36780 tcctgcgggg attacgtgct gctggacatg caggagcgcg ggctggaggc cttgccggag 36840 tacagcatgt cgaagctggc cgtgtacttg ggccggcatc tgcggcacgg tatcgagctg 36900 gcggtgccct ccgccgcgtc tgctatgcgg ttcctgcgtg cgcttgccgg catgatgccc 36960 cgcgatgagc ccatgcgctg ggtatcacca gcgggattcc ctgtgatcca gcattacgcg 37020 gccgaggagg ttgtgcggtt tgacctgcaa gggttggggg tcatggtgag catgacgcgc 37080 tttgacgata accgactgaa tcgctccaag tgtgtgaacg gcatcgcccc gaacttcgtg 37140 cacagccttg acagcgggca cttggtgcgt gtgctggccg cgtacaaggg cagccttgtg 37200 cctatccacg attcgtttgc cacgcaccca tcggatgtgg atgacatgca ctgggtgctg 37260 cgcgatgagt tcgtgaagat gtacgaggag tcggacccga tggacgcgtt ggtgcagggt 37320 gtgcagcaat tccgcgaaga ggtgatcgag cagcctgtaa ggggcacact agacatctcg 37380 aaggttcggg attctgtgtt cttcatgtgt taggaggtct tatggatata caacgtctgc 37440 gtgaacttta cgcagtaagc tctgatagcc catccggggt tgttatggca gcggataggc 37500 gccgcgggtc tggtaagaag ggccaacccg cattacatac cctgcggaac aacggctgcg 37560 ggggttttta ctatggcggc gcttatgtag aaaccacatc acaaggaaag gtgcgccacc 37620 atttaagcgc acaccgtgtt gtgtttgccc tgacctacgg gtactggccg gagacggtag 37680 accacgtaga tggcaacacg ttaaacaacg accccttgaa tctccgcgcc gctacagcgg 37740 cccagcagca ggccaacaaa ggcgtctcta ggcgcagcgt tacgggtctg cggggcgtgt 37800 actacaggcc agaggacagc gcagtacgcc cttacagggc aatgtgtaaa ggtaagtatt 37860 taggtatatt tcatacgcca gaggaggcgc atgccgcgta ccagcatgca gcggcggaag 37920 cctacggcga gtttaatagg gcgacacgat agtgtgggtt tgactctgca cttagtgtac 37980 agagagggat gacggcacag gatgcggtcc ttctctgtaa agtgagacgt acctaagagg 38040 tgaattgcga atgtcttctg atcagaagaa agtaacgttt tcagtagagc agattgattg 38100 gttggagcag cagttcccag agctgcagtt cctacccgag gcatccctag gggagcttca 38160 gcatgctcaa ggccagcgat cggttatcca ccgcatcaag caggtcagtc gggtggaggc 38220 acggaatgtc ccaataccag ttaaagtatc atgacatcgc cccagaaggg ttgatccgtc 38280 aggtagctga tatcgtctgg gtgcaccccg cgtacaacca tgcgcgagcg ctgtaccctg 38340 atcccgagag cttcactgag gctatgctgg ccgatctcca tggattccct gcacttgcag 38400 cgtattccgg ggattctcta gcggctgccc tgtcgttcac tgatcttgtc cgggacctgc 38460 acatgccggg cctaggcagc atcgtcggca gcagcatctg ccacccagaa tatcccgggg 38520 cagccggttt cctgctgagg gcgtacatcg ccgccctgcg tgctgagggt gccaagtggt 38580 accagatcag caagcggctc gatgccacaa cagtcctcac caaataccgg aggctccatg 38640 agtaaaaaaa tcaagagcgc tttcgatagc gtggccaagg tagcaacctt cgggttggca 38700 gacaccaaga agcttaccga tgtgttcacc cttggcggtg gcggcggcct gctcgatggt 38760 atcactggtc aagcggctat cgacgcggct aaggctcagg ccaagaccca agaaaccatt 38820 gcccagcaac aggccaccat cgcccagaac gctgctcagc tgtccgctgc caacgcggta 38880 gacaactcgg tgaacgtgat tgccggcggt actgccaacg ctgccgactc ccttggaggc 38940 gacttcaagc gcaagcggac gaattccatc gcgtcccaat tgggggtgta acatgaaggc 39000 accagtcaca tacgagagct tgttcaccaa gtaccgtgac gacagcgccg tactgcggaa 39060 cgagcagtat gcgctctgga gcatccccac gatctactcg gactcagatg tccgcacgga 39120 cgggaagcag cagagcgtac gccgcgactt ccagtccgct ggcgccatgc tggccaacca 39180 cttggcgagc aagatcgcat cgctgctgtt cccgtccact cagtcgttct tccgccttga 39240 ttcggcagtg aaccctgacg agatggcctc ggcactggcc gtggaccaga acgagctggc 39300 ctctgggctg gccacgctgg agaacactgc gtaccgccgc atcttcctcc gctcgtcgta 39360 ccatcagctc gtgcatgcca tgaagctgct gatctgcacg gggaactgcc tgctgtaccg 39420 cgatagcgcg aacacgaata tgcacgccta cagcatgcgc tcgtacgcca tcctgcggga 39480 tgggtcgggc aaggtgctgg atatcatcct gaaggagcgc accacagtcg ggcagctgcc 39540 tgaactcctg cagagcacgg cgtatgccgg gcgcgaggag tacgatagcg tgtgcctgta 39600 cacccgcatc aagcgtgagc gccgcaaagt caccgacgta ttcgtggtga cccagcaggc 39660 ggactcgcat gcgctggatg tgcgcgagga gtacccggag gcagtatgcc cgtacatccc 39720 cgtaacgtgg aacctcatca ccggtgagaa ctacggccgt ggcctcgtgg aggactacgc 39780 cggcgacttc gccaagctga gcgagctgtc cgaggccctg gccctgtacg agattgaggc 39840 atgccgcgtg ttgcatatgg ctgccccggg ctctcaggcc gacatcgatt cgatggcatc 39900 cgaggagatg ggcgcttggg tagcgggtga ccccaccaag gtgcaagcgt acgaggctgg 39960 cgactacaac aagattgtgg ccctgaccgc ggacctgacg caaatcttcc aacgacttgc 40020 gccggcgttc atgtacggcg ggaacgtgcg agatgccgag cgggtaaccg ctgaggagat 40080 ccgtacgcag gccgaggaag ccaaccagtc gctgggtggt gtgtacagcg tgatcgctga 40140 taacctgcac atcccactgg cccacatcct gtgtgccgag gtgaaccctg acttcgttgc 40200 cgaggtaatc gccggcggcc tgaccttgag tgtactcacc ggcgtcgccg ccttgggtcg 40260 cagctccgat gtgaataaac tcctgcaggt tgcacaggtg ttgggtgcca ccctcccagt 40320 attcactcag gtgagccagc ggctcgaccc tgagcgcgtc atctccaagg tgttcgaagg 40380 ctttggcctg aacatcgaag actattccca taccgcagaa gagttggaag ccaaacaggc 40440 cgctaacgct gcacccgtgg acactgccct gaccggtgcc gcagctgaca tccaaggagt 40500 cctgtaatgt ctgagaacca gacccaagat caagtaaacg agttcggcag caagatcagc 40560 cccaacgctg acgcgaatgt tgccctgaac gagaacggcc ctgatcaggg tactcttgct 40620 caaatccttg cagccatccg tggtaaggac gttgagacgc agccagctgc cgccgacaag 40680 ggcaacctcg gtgttgatgc tgatgccgac gagaagccac tgccaaccgc tgacgacgag 40740 gacttcaagt tcgagaccgg caacaaggcg ctggatgtgg ctgtatcctc gttccttcgc 40800 agcaccggtg caagcgatgc cgacatcgag cgcgcatgca agtccgctct ggagtatggt 40860 gaccctaaac tgatcgaccg cgccttcatt gctgagaagt tcggtgaccg cgcagcggac 40920 gccgtggctc tggccgaggc cgtaatcgag caggccgggg ttgagaaaca acgtaccgtc 40980 caagctgtgt acgatctcgc tggctcgaag gagcagtggg acaccgcact gggtgtgtac 41040 aagcagcacg cccccgctgg cctgcagaag gcgctgggga ttatgttcag ctcgggagac 41100 gccgcatccg tgaaggaagc agccggtctc gtgttggagt ttgccaaagg cagtggagtt 41160 ctccccgtgg gtgcggggca acgccaagtg gctggaggcg gtaccgctgc ctctgaaggc 41220 ctctcggctc aagaattcaa ggccgcactg agtaagctga acccgaacgc acgcagctac 41280 catcaggact atgagcgcct gttgaacatg cgtcgtgtcg gcaagcagct cggcatgtaa 41340 tctaatccaa ggagaatcat atggctaaca ccgcctacac tggcaacctg actcgcccgc 41400 actggggtgg tccgaactcc gacgctgaca tccatctgga ggtcttcgac ggtgccctcg 41460 acaccgcgtt ccagtacaac gcgttcttcc gtgccaacag caccttcatc tcggtgaacg 41520 atcgttcgaa caccgcccgc atcgaccgtc tgggcactgt gaccatcaag ggtcgtaagt 41580 ccggcgaggc gctcgatcct actccggtca agaacgacaa gctggtaatc accgtggaca 41640 ccgtgaccta cgcacggtcc ccgatcgatt accaagacga ctggaccgct ccggacttcc 41700 tgccggctat cggccagaac cacggtaccg agcacgccaa gctgttcgac caagcgcaca 41760 tcatccagct gatcaagtcc cgctcgtgga ttgccccggc tcacctgaag ccagccttct 41820 acgacggctt cgagaagacc gcccagctgg tgaccaacga cgctgctgcc tcgtctgacg 41880 ccatcgtcgc tgcccacctg agtggcatcg agaccctgat caaccgtgat ctgggtggct 41940 cgctgaacga gttcatcact ctggtgaccc cacgcgtgtt ctcgctgctc ctgcagaacg 42000 agaaactggt gaacgtccag tacagcgacg gtaacgcaaa ctacgcccag cgccgtgttg 42060 cgtacctgaa cggtacccgt ctggtcgaga ccgctcgctt cccgaacgct gtcatcacca 42120 accaccagtt gggcgctgcc ttcaacgtcg actccaccga catccaagta gagatgatcg 42180 tgtatcatcc gaagatgact ctggtgaccg ttgaggccaa ggcgcacacc tcccgcgtgt 42240 gggacgatga gctgaacttt gcgaacgtgc tcgactcgta cgccatgtac accatcggcc 42300 aacgccgccc agacactacc ttctcggtac gtctgctgcc tggcgcgtaa gacccttggg 42360 cccggttcgc cgggccctca ctatttagga gacctgccat gacactttta gaggcggtaa 42420 acacctgtct gtccgccctt ggcgaatcgc gggtcaccag caccacagta gtgcacccta 42480 cggtggacct cgtcctgagc accatcgcca tgaagcagcg ccagctgctg gagcgcgggt 42540 ggtggttcaa catgagccac gtcaccatgt acccatccac cacgggtgag atggagtacc 42600 ccgccaacgc cattgccatc gtcggtgatg agggcgaaat cttcgtgccg cgcggcggca 42660 tgctgttcga tatggacaac aacacccagt atttcacgga gcccaagagc atgaccgtgg 42720 cgtacaacct cgacttcgag gaccttcccg agtgtgctgc aaacgtcgtt atgacccgcg 42780 cggcgcagga agtgtattcg ggtgaccttg gcccggacgc tgctgtgcag cgcctgcagg 42840 gccttgagat catgagcttt gccgagatgg agttgcagca catgcgcaac cgccggtaca 42900 gcaccaaaca acgtcgcgga tggggtcgtc tcgtccgtgc cctgcgcggc taggaggcac 42960 tatggcggct atcgacggcg caaccaaatc gttaatgcag ggcgtgtccc agcaagtacc 43020 ccgcgaacgt ctcgacggcc aggtctccct tcaggtgaac atgctgtcgg acgccgttga 43080 aggcatgcgc cgccggcccg gtatgcggta cctgaccagc aacatcttcc cgggcggcgc 43140 ttggaacaaa gagcaggtgt tcgccacctc cgtggacatt ggggacgagc cccaccacat 43200 cctgatcaac accgccaccg gcgttgtgaa cgtgtacaac agcaatatgt cgctggtggc 43260 tactgacgac ttcttctacc tgctggcgcc taccgctgcc agtattcagg tagcggcatt 43320 acgtgggtac ctgtacatct gcaacactga gcagttccca gtcaagaacg tgactaatgc 43380 cggcaagcag aaccctgaca agacgggttt cttctttgtg aagacggggc agtacagcaa 43440 gatttacaac gtcactctga acgtgaacgg ggtgcagtac gcctcatcgt acactacacc 43500 gaacggatcc gcccccggcg atgccgccct gagcacgcct gagtacgtag ctggcaagct 43560 ggtggacggt attgtggcac acgccattcc gggcctctcc tttgccatcg cgggctcgta 43620 cgtgtacctt gtcaccacgg gtgctggggc catcactgtg acctcagatt ccggcacaaa 43680 cttcatcagc gcctcgaacc agtcccatgt caccctgact actgagctcc ctgcccggat 43740 gcccgcagct gccaacggca tgctggtaag cgtgggcgcc aacccatcgc tatccgtgtg 43800 gtaccgcttt gacttcgtga acaacgcgtg ggttgagtcg ggggcttgga acagcgccga 43860 cttcctgacg aatatgccca tccggttgaa gctggataac tcctacgtga tggaccagcc 43920 aaactatgag gggcgtttgt cgggtaacga caacacgaac gaggaccccg ccttccttac 43980 ggacggcgtg accggtctga gcgtattcca aggccgtctg gtaatcctaa gcggggcaac 44040 ggtgtgtatg agcgctgcgg gcaaacccct gcgctggtac cgtagcacca ctacggaact 44100 cctcgttgcc gatcccatca gcatcttctc aggctcggct accactgcca acttcacaca 44160 cgccatccag tttaacaagg acttgttact gtttgccaag acctgccaag cggtagtgcc 44220 ctcgggtaac tctgtagtgg cgcccagtac ggcccagatc gtcatcacca gtgcgtacaa 44280 cacaactagc aaggtgctgc ctatcgtggc agggcggtcc ctaatgtact tcgcaccccg 44340 cagcgagaag ttcgccagcg tcctagagat ggtgccgagt aacaccacgg actcgcaata 44400 caccaccaac gacgtaactg cacacatccc acagtacatg ccggggacca ttcggcaggc 44460 tacggctagc accaccagca acagccttgt gcttgtcgct gacggggatg cccgcaccct 44520 gtttgtgcac gagtacttgt ggggtgggga tgagaaactg cagtctgcat ggcataactg 44580 gaccgcaccg tatgacatcg tgtgtacttg gtttgtccgc gacacaatct acgtcggtat 44640 gttagtagac ggcgacctcg caattgtcac gatcgactca caggcaggcg acacagccaa 44700 cggcctgtac cgcccgttct cggacatgtt catcggcact gcagtcgtcg gcggcacctt 44760 tacggttccc acggcgctgc gcgctgcata cacagccgga gccgagttgc agttgaccta 44820 caccaccggc gcagttgctg gcgaggcagt aggcttcagc atcaacacag ccacttgggt 44880 tggtacagcg gtgcgtaacg tccctgacgg cacgtacttc ctaggcctca agtaccgcag 44940 cacgctgtca cctacaccac cgctactgcg cgatcagaac ggtgtggtga ttggaaccgc 45000 aggcacatcc ctgatccggt acgaggcctc cctgaagaac acgggtgatt tccatgtggt 45060 tgtgcaacgg cacgacgaga ctacctcgga aggggtatac tccggcctta catacagctc 45120 gcaggacttg atccccggtg ggcccctgta cgccaagagt agccggtgca tcatcccagt 45180 gcgtttgaac cccgacgcct ctagcgtgat catgtacgca gaagacgatc acgacctcgg 45240 cgtgcttacc cttgagcacg ttatgcagta ccaccctcga cgacggagaa tgtaaatgat 45300 ctggatggcc gcagcagcgg cgcttcaggc agtgacccag ttgggggctg cccgggggca 45360 ggccgctatc gacaaagcaa ataaccgcgc cattgaggcg tacaacaaac aggtggc 45417 <210> 2 <211> 2730 <212> DNA <213> Pseudomonas putida phage <400> 2 atgccagtac gccaagatgt acaaggcggc ccgcgggctg ccggtcttga gggttatcag 60 agcccgggag ccgcccgtgc gcagttccaa ggcccagacc tcagcggcgt acagcgcgat 120 gccgcagacc aagccaactt ctggacccgg ttgatcggcc gtggggcgaa cgccctgcag 180 gctatggacg agaaagaccg ggcccgggcc tatttagagg gtcagcaaga cagcgccatg 240 ggcaaggccc gcgctgaagt gtatggctca atgcagtccg actacgagca aggctataac 300 cgcgccacca ttggcagcca gttggcgaag taccagctgg gcgtgcagca agccgccatc 360 gaacatgtga actcgggcaa gaccccagag gagttccagc agttcgtgga ggaggagacg 420 aactccctgc tgaacgcagc cggcactcaa ggccttgatc tgaacgagaa ggactggcag 480 gcgtggctga ccggcgtgca gagcacccgg gccaccgccg ctgatctgtt ccagaccaag 540 agcgtagagc gtgcccagta catgaagggg caggccatcg ccgccgaggg caacgccagc 600 attgctacct tcatggctgc cgatgaggcg ggtaacccac tgcaggcgct gggcgggatc 660 acctcccaca ttgcccgcgt gtactccgac aacaccttgt cgcctcagca aaaggacggg 720 gcccttgcgg acttcgctgg ccaactcatg gcaagcgcta agtcgtccgc cgccgttgag 780 gcgctgggct cgtacttcca agagtcggcc gagttcaaat ccctgcctac tcaggtacag 840 acgcaggtta tcaacggcgc ccagcgggcg tacgagcagc gtgcagccga cgagtcgggt 900 gcggtgtact cgtacgtgtc gaacgtgcgc gctgtggcca acccggatga gctggagcgc 960 cagttcccca tggcgtcctt tattagcacc ctgaacgagg cccagcgggc tcaccgcatc 1020 actccggccc agatgtacgg tatggtggag gaggagaaca ctcgccgcct gaagctcggc 1080 aaggctgccg cattccagca gaacctgctg cacggcacca ccatgtccga tatggcagct 1140 ggtaccggtc agactctcgg caagatcaag actgccctca cgcaggtata cgccaccagc 1200 aacggtggct acagcggcgg cgggctggcg ctcatgcagc gtggtctgca gagtggggcg 1260 caggacatca cagctgtggg tatcgagatg ctgcagcaag acgcacagtc cctcggcagc 1320 atcgacagcc ggaacctgaa gcgcgatgct gacgggcagg ccctgtaccc ggctaccgtg 1380 gtgaactcgc tgacgaacct gaagcgtgcg tacgacgctg cacaacgagc cggcaacaac 1440 gtgcaggctt ctcagctcat tgctgggctg cccgacgctg tagcctatgg catccgccaa 1500 gccagcgacg agaatagcat tgcggacgtt gtgtaccgcc gggccgatga tatcgcctcc 1560 gggcgcgtgg tctccctgcc ggctgccatg cccaaggaaa tgctcgccac tgtggacgat 1620 gtatccgccg gcatgttcga cacgaacctg acccagaagg gcgcggctcg gaacatcctc 1680 ggcatccagt cgtacgtgtt caccagcaag gaagatgccc gggcgcagga agcccgcttg 1740 gcgcaggtga acggtgccct cggtgaggag tacacctcac tgtaccagca aggccgtctg 1800 ccagcgctcg ccggcgacga cctgaagaac tggctggtgg gtcgtgtggc tgcccgtacc 1860 gtgcgtgtag acgatggcac cgacaacggc acactgctga tcctgcctaa cgtggccaac 1920 cgcgcccaga cctttggcag tgaggacaac aacatcatcg gccaaggcct gaaggactct 1980 gttgacgagt tccgtgcagc caatcccggc gctgtatccg tccagatgcg gtacgacccc 2040 atggctggcg agatcgtgtt cagcggcact gacaaggaca acacggtgct caccacttcg 2100 tacgccgtgc cggtcgatca ggttgtacgc caagtgcgtg cagttgagac tcgcctgacc 2160 gcgaacggta atggtaacag catgggctcg ctcgctgtgc ccggcgccgg gtttgtgcag 2220 tttaatacgg ccaacgccta cggaatcgag ccgcaagtgt atcaaaatgc agttacccag 2280 ctggtctcat acgagggcta caccgacacc aaaggcttca gcatcctggc cacccaccca 2340 accacgggtg cggccctgaa cgaggccaag tacgtgaagc agcccaccga cacgccacag 2400 gtcgctgcga acaagctgca actgtacatc caagacaagg tgcttccgcc tgtcatggct 2460 gagttgccgc agttcaacgc cctgcctgag cacatccgtc agcaggtgtt ccagcagctg 2520 gtggagacga cgtatcacgc cggtaacgcc aaggccttcg gggacatcct gaagaaggcg 2580 ctggatggtg acacactggg ggcctaccaa gacttccgcg agtccgccct gtacaaggac 2640 gctgggccta agtcccgccg caaccgtgac cgactgcaaa cccttgacgc tgtatcccaa 2700 tacagccgcg tgcaacgcac cggctactga 2730 <210> 3 <211> 3795 <212> DNA <213> Pseudomonas putida phage <400> 3 atgagcatga tccgtttacg ccctgacgaa gaccttgctg tcgcaccggg caccgccccg 60 actgtcggcg gcgggttcgt ctccggcgct gagaacagct tactggaacc cggtacagca 120 aacacctcgg cgctgaaagt agcgccgacc tcttctgcag agagtgccga gggcgctctc 180 cgcgaaacca tcgcagccaa ggcgctgcgt ggtatcgagt acatgcaaat cccaagcgag 240 gaggggttcg accctgcagc cgcgctgggc gacaaactcg gcgccttcac tgaggaagag 300 ctggagttcc ttggcgatgc ccgctccagc accgaactgg cccagcgcct cggtcaagtg 360 acccagaccc gccagaacct gcaggacatg gccgccaacc ctgtaaccgc cttcgccgcc 420 tcgatgctgg acatcgacgc tgtgatcggc ctcggggtag ctggtgctgc tacgcgcgcg 480 ggccgtaccg cgcgtctggt tgccggcctg gccgctaaca ctgcggtact gggcgctgca 540 tccgagggcg gtgagatcac cccgctggat gtcatcggca catcgctggt cgtagcgctg 600 ggcgcaatcc ccaccgtacg ccgtgcagca accgttgccg aggacgcggc tcaggacctc 660 ccaacaggcg cgcgtagcgt tcctgacgtt gaggaggtag tacccccacg ccccgctgct 720 gaagtcgctc cagatgtcgt ccctgacgct caggtggccc cttcccgcat ccctgaccca 780 gactatgaag tccctacccc tgacatgacg actgtacgcc cgtacgtcga agtgaatcgg 840 gtcaagggtc aagccaccat ccgcaccagt actgcgaacc ttgtgggcgc cacactggcc 900 aatgccagcg acctgcccga gggtacccgc cttctgggtg tagccttgta cgatagcatg 960 aagctggacg gcgacgtgcc cctgatcatc acccgtggca agggtcgatc gaacgtccgc 1020 ctgtggccag acggccgcct gcagaccacc atgcgcacag ccgagggcgg ggcacctacc 1080 ctgactgagg ccgtgcagaa catgagcacg tacgagaaga ccgttaacct gcacgaggca 1140 gcacacgcca agaccatcac agcgattgcc ctgttccaac agggtaagct ctcggctggt 1200 ccagtgttcg acgcagtgca gcagatcgat caaatccgca acgtcgtgcg gggcaacatc 1260 agtaccccaa ccctgcgtga gggcatcagc acttccgact ggaagaacaa cgtgctgtac 1320 ggactcagca acacccacga gttcgtgtcc cagctgttca acagcgagtc cttccgcaag 1380 gtactgcacc agatccctat gcccggtgag ccccgctcag tgtggtctag cttggtgcag 1440 aaggttgtga ccgccttcac tggcaagggt cctaccgaca ccgcgctgac ccgcttggtg 1500 gctgcattcg aagacctatt gcaacagccc tctgtgactg acgcgcagtt cgtgaaggcg 1560 tacaacaagg ccgacgtgcc gcgtacccaa tcggccgcgc tggctaactc cccagacatc 1620 cagaccatgt tcacccgcac cgggaactcg ttgaatcagg ccttcgcctt gttcgacaag 1680 attcgcgcca tcggcccacg ggccagcacc cttgcggaac aactggttgt cgatgccact 1740 gggaactcgg ctacgtctgc tgcgcattac gcccgcactg cccacttggc tgccaacgtg 1800 gccgctgcgc aggttgatgc tgctgtgcgt caggccctgt ccgcccaagg ctggactatg 1860 ttctctcgcc tgcgcaaccc agctgcattc cgggctgcac agcaggagct gtccggcaag 1920 gtgtacgacg ccctgtccga taaccatgcc cgcttcatgt cgggcggcac cgtgaaccct 1980 catccagacg cagccgtgca gcgcatcgtc gatgccttcg ccagctcccg ctgggctgag 2040 gaccagctgg cccgcatcaa ggcctctggc atgaccggtg ccgacaccat cgaagcgtct 2100 ccgtactact tgccacgccg ccacagcggt aacaaggtga gcgacttcct ccgtgccaac 2160 ccgggcgtta ctcgggcgga cattgaggcc atgtacggtg agcagttcaa gttaatgttt 2220 gcagaccgcg gtatccgccc ggacactgcc cgcgcgctgg gtcgtcagat gctgcggaac 2280 atggatgagc gagccgccgg cgtgcaaggc taccgccagc acatcgcagg tatgaccaac 2340 gacgacatcg agttcgctat gcgcaacgcc ggtatcgacg aggatcagat caacatgttc 2400 ttgaacactg tgcagaaagc gggcgagcaa tccaacaccg tgcggaacct ccgccggcgc 2460 gtggactttg acatgacccg tgagtacgtg accaagagcg gcgatgtgat catgccgcag 2520 atgttcgtgg acaaggatgt gctgggcctg atggaaggct acagccgcac catgagtgga 2580 cgtattgggc tggccaaagc cggtttccct gacgtgcgta cgctcgccgg cgcagtcgat 2640 gaggctgccg ctgagggcgc agacccacgc gcagcgcgag ccacgttgga caacactgtg 2700 aaccgactgc tgggctaccc cactggggaa gacgtgcctg atatcctccg cagctttgct 2760 gtggcgtctg gtgcggtgca gctggccaac tctggtatct accagctggc agacactgcc 2820 ctgatgatca aggagttcgg tattgccaag gtactgcggt ccctgagctc cacagagtgg 2880 ggccgtgacg ctttgaagct ggcacaggac ccaacctacg gctctcgcct ccgcgacgtt 2940 ctggaagcac gcaacgtgct ctcaggccgg taccggacag tgatgaccca cctcgacgac 3000 aacaccgata tcggaaacct cggcatgctg caccagaaca tccagcagct gggtcaagtg 3060 acccgcttcg ccaacggcat ggagttcgtg cgccgcgggc agtcgaagat gatggccggg 3120 ctggtaggtg acaccgtgga tgacgccatc cgtggcaacg acgcggcatt cgagtccctg 3180 cagcgctttg gcatgacgcc tgagctgcgt gcccgggcta aggccgcgat ggatgccgac 3240 cctgacatgc ggatgtggcc agacagcctg cgcctcgaca tggagactgt ggcgcacaac 3300 atggctgacg cccttgtcat ggagaaccgg ttgggtgagc ttcctgcttg gatgcagttc 3360 agcgccctcg gcaagttcat cctgccgtac atgaacttcg tggctggtac ttggaacaag 3420 atcctgcgcc gcacgtacgc caacgacggc cttgccggcg tggctatgat gttcgcgtat 3480 caactcccgc ttacaaccct ctcgtcaact gtagcgcttg cgcagggcaa caaggagatc 3540 actcctcaga ccctggccgt gaacgtgctg acccaaatgc cgctgatgtc gtggctcggg 3600 tacgctgtga acatgatgac tcaaggcccg acgaacagca ttgctgctct tgggttggtc 3660 gataaggcat actccgctac cgcgtcactg gtgcgtggcg agcctgatcc agagcaaatc 3720 atccgcgcaa cccctttcct cgggatcatc cccggtatcc gcatcatggc ctcggccttg 3780 ggcgaggacg aataa 3795 <210> 4 <211> 2175 <212> DNA <213> Pseudomonas putida phage <400> 4 atgaacgcca tccaaactga ggtatccgac ggcagcttag tcagcgttaa cgtcggactg 60 gaatacttcg agcagtcgga tatctcggtt ttcttggact tgagtatcgc gccacttatc 120 gaaggcactg actacgcgtg ggccaacgcc acccgcattg acttcctgac aggccctgtg 180 gccgcaggtg tggcggtgac attggttcga aacaccgaga acgaggcaat gctgaacatc 240 tacgatggcg gcgccccgtt caaccgattc acccttgacg agaacttctt gcagctgctc 300 cggctgtcgc aagagttctc cgagggctta ggcttgaccg gactgcggca ggccttggac 360 atgcacggct accgcatctt caaccttggt aacccggtga atgcacagga tgctaccacc 420 aaggcgtacg tggacctgct gatccagacc cttgtggaat cgggtaccgg cccaatcggg 480 gccgcctcga atgtcgtgta catcgaccct gaaggccagc taaagaacct gcagcagctg 540 tctggtctcg atggtgcgga cctgatcggc tggaatggca cgggcctggg tccgttcctt 600 gaagccggct actacgagcc gcaagggctg gaccttaccg gtgttgtgga cgagtctgcg 660 gtaatcaacg ccgcgctagt gaagtacaag cgcgtgcgtc tcccggcagg caccattaag 720 gctaacgtgc tcgtgccatc cggctgtacc ctgcggggcg ccggtaagac ccggctgaac 780 cgtagcacca aggtatgggc cgccggtggc accaccatta tcggctctgt agtggcgtcg 840 aaccagatca actggactgt caccgacatg aacatcgacg gcttcgccgg cggtaacgct 900 tttggtgctc agggcccatc cacagagttc gggtacatcg gtcgagtggc aatgcgcggt 960 agtgaccaca acatgatcct tgagcagaac ggctctgatc ttgcaggccg cttcggcggc 1020 aatatcctcg tcgaggactg cgatgcgtat gatggcccta acgggtttgc tgtgaagatg 1080 cggaacgtga ccttccgccg gtgctacgcg tacgacatca cagtgcaggg ctttgtggct 1140 gtgtctgaca acatcaacgg cccggctatc tactcgcgcg cgcagaacgt gcgctttgag 1200 gattgtggcg gcgacagctg caaccaaggc tttgtggtgt attcccgcga cttctaccac 1260 gggaatttcc ccggcagctg ggtcgccacg gtgagtcccg ctaccgacat ctactgggac 1320 ggtacagtca cgaacgtgac ttcgggccaa gtacacatcg gtgacttcag ctcggacggc 1380 accttcgcca ttgtgttcaa caacgagatc gagattggcg gcgggcacta ccagcactcc 1440 ccgctgtttg gggtgcggtt cgactgggcg gcacgtccaa aggtaaccgg tggccacttc 1500 tccgggtgcc cgaacccgat cgtgcgcggc acgaacgtgg tagacctcaa gattggtgat 1560 ggggtgacgc gcttcggtgc catcaacccg ggcatcctgt ctgagcgcat tatcgagagc 1620 tcaaatacca gtgccgtgaa catccagaat gtacgccaag tgctggtgtt ccagaacacc 1680 gtgccgaccg tggttacatc cctcgtcacg cctgatacca accacaccat tgacgttgtg 1740 attgacgacg gcgttacctc actacagctg gcgggcatcc tgctcaccgg tcggggcagc 1800 cgggcgcgct tccagtggtc ggcagatacc agcacttggt tgtgcctgat gagtggtagt 1860 gtgctgccac cgggcgagat tgcggttccg tacgccttga gcatgtctgc cgattggcgc 1920 gccctggcaa actccatcgc catgaacggt aacatcaacc tgctggacgt acctgtggcg 1980 aacatcgccc cgggcactgt agcccacctg cgcatcgtga acgttgccgg cggctcacgc 2040 agtgtgttca actggtcagg tgtaacgtgg ggtgccatca cccccgtggc tgccattgcg 2100 gccggtgcca ctgtcctcat gaccctgtac cacaatggaa gcacttggtt agtcaagagt 2160 gcccagacgt tctaa 2175 <210> 5 <211> 294 <212> DNA <213> Pseudomonas putida phage <400> 5 atggctaagt cagcaagcaa gagcctgctg aacgagctgc acgagatgct ggcgcaagtc 60 ttcattgacg acctgcgcca gtcgcgggag gagggcattc ccctccccgc cgctaacctc 120 ggtgtgattc ggcagttctt gaaggataac gatgtgagcg cgagtatcga tgctgacgac 180 atggcgaagc tgcgtgacac cttcgcagac gagttggccg agcgccgcgc actgaacaaa 240 gcccgattgc agcagacact gcaggaggac gatatcaacg agttgcttgg gtaa 294 <210> 6 <211> 1815 <212> DNA <213> Pseudomonas putida phage <400> 6 atgattcgag cgcacacact agcgcgcctg cggctgctcg tcgagcgcac gaacaagtgg 60 aaggacaaca gcaacgccat ccccgctgag gagcgagttg atctcgccct catgttcggg 120 gccacattcc cacgtttccg ggagttcgct gagctaggga tgaagttcct cggcttcgag 180 ctcacggaga tgcaaggcga cattgcagac tacatgcagg atggcccacg caacagcatg 240 gtggccgctc agcgcggtga ggccaagagc acccttgcgg ccctgttcgc tgtgtggtcg 300 ctcatccggg accagagcat gcgcatcctg attatctcgg gcggcgagaa gcaggcttcg 360 gacgtagcca tcctgatcat ccgcatgatt gagacttggg gtttactgtg ctggctccgg 420 ccagacaaga cccgtggcga ccgtacctcg tacgagagct acgacgtgca ctgcgacctg 480 aagccgctgg acaagtccgc ctccgtggcg tgcgtgggca tcaccgcgca gctgcagggt 540 aagcgggccg acctgctcat tcccgatgac atcgagacca cgaacaacgg cttgaccgct 600 accagccgcg aactgctgct actgcgctcc cgcgacttcg cggccatcaa cactcacggc 660 aagaccctgt acctcggcac gccgcagacc aaggactcca tctacaagac cctcgctcga 720 cggggattcg aggtgcgtgt atggccgggc cgcgtgccca ctgtggagga ggctgagcgc 780 tacggcgaca ccctcgcccc atacgtggct gccatgttcg agacccatgc ccgcactggg 840 ttcgggctgg acggcacccg tggcgaggtc acagacccgg gccggtacac tgaggaggac 900 ctgcagagca aggagctgga ctttggcccc gagggcttcc agctgcagta catgctcgac 960 acgtacctcg tcgatgccat gcgcacgcgc gtgaagctct ccgatgcgat tgtagcatcg 1020 ttgggcaccg atgctgcccc tgataccctg tactacgccg caacgccaca gcaccgcctg 1080 caggagctcc cagaggctat ccacggggag gtcatgtacc gggcggctgg cgccggcgag 1140 ctcatgctgc cgtaccagca caagatcctg atggtggacc cggccggttg cggtggggac 1200 gaagtggcct tcgccgccgg cggggcgctg aattcgtaca tccacctgtt cggtgtgggc 1260 ggtctgcagg gcggtatggt cgaggagaac tgcaatgcct tgatcgatat ggctgaagaa 1320 atgggcatca ccgacatcgt gcttgaagcg aacatgggcc acggcaccgc atcactgctg 1380 ctgatgaacg cccttgccaa gcgcaagatc acgaacatcg gggtgcgcga catctacgcc 1440 accacccaga aagaacgccg catcattgac acccttggcc ctgtgttccg ccgacacaag 1500 ttcgtcatgc acgagcgggc catagagatg gacgaggaac acctgcaagc gtacagccgc 1560 gagaagcgcc gcctgtacag cctactgttc cagctgcaga acatcacgta cgaccgcggc 1620 tctctggcca aggacgaccg tgccgacgca gtagctcacc tcgtgaacga gctcaaagga 1680 tacctcgcgg tggacgagga gaaggaagca gagaagttgc aagccaaagc ggcccgggag 1740 ttcattgaca accccatggg ctacaccaaa gggatgggcc gacgccccag tggcacccga 1800 tcccgtctac gctaa 1815 <210> 7 <211> 543 <212> DNA <213> Pseudomonas putida phage <400> 7 atgagcctaa agactaagat cgtcacggcc ctagcagcga gcacgctgct ggggtccgcc 60 ctgcttacca gcctgaccag ccttgagggc accgagtaca ccgcgtactt cgacagcgcc 120 ggcgtcctca ccatctgcag cggcgacacc acggacgtca ggaagggcca gagagcctct 180 cctagcgagt gcaagcaaag gctgaaggat ggtattgccc agcatgcgaa cgcgctccgt 240 gggctgcctg agagcctccc agacacggtt gtactaggtg ctgtgagcaa cgtgttcaac 300 atcggggagt acggctttaa ctcgtcctcc cagaagaagt gcttggcccg gggcgactac 360 gcctgtgcta aggcagcaac cctgcgctgg cggtacatca ccgtgaacgg caggaagttc 420 gactgcagca cccggggtaa ccgcgtgtgc tggggcctgt gggagcgccg ccagtgggaa 480 gctcgtgcca tggggaatga gttcaagacc gtgcaggagg cactggctgc cctgccgaag 540 tag 543 <210> 8 <211> 951 <212> DNA <213> Pseudomonas putida phage <400> 8 atgacgccat caacgcactg ggagtattgg gatgaacaac aaggtatcac tggccgcatc 60 cgtaatgtgc ggcctgacgt tgggtgccct catcgttgcg gctatcacgg gcggcttatg 120 atcccccggg cggagtggct gaccgcagcc caagccctcc cgattggtgg caagcgcagg 180 atcaagcacg gcttcgaggc caccgccgcc atggatgtgt ggaacaacga agatagctgg 240 tcctgctact gccatcgctg ccacgagggc gggcgagtgc cgaaggaaca tcagcgggtg 300 cggcgcgtca tggtcgagcc cgaccgcgta gcgcctgtac ctgcaacggt aattcgcttg 360 gcagatgcga gccgctggga acagcagcgg atttgggaat tactgtgccg caagggttgc 420 ccgccgggcg taatccccga ggaggtttta tggtacgaaa gatcagtgga tcgtctgatg 480 ctgctatcag acgacgtggc gctcgggagg gcgctggacg gcaggcggat gccgaagtgg 540 ttaccgtatg gggcgtggcg gggcaagccg atgatgtggg cgactcgtac cagtgccgag 600 gcaacggaac ctgcggactc tgcgccgctt gggtcgctga tcctgaccga ggacgcgctc 660 tctgcgtaca aagtggccaa ggccatcgac acctatgcgc ccagaagctc gctggacgta 720 tgcgcgaccc tcgggactag catcaccgac cggttcctac cgtacacact gggccggcct 780 gctgtgctgt gtatgtacga cggcgatgcc gccgggctgc gtgggtacag ggccatgcgt 840 caacgtctgg ctgtgtggca gcagcccgtg ttggacctac gcccagagca gggcgacccg 900 aagaacaacg acctagcatc tatagcggag gcactgagta agtggatttg a 951 <210> 9 <211> 1197 <212> DNA <213> Pseudomonas putida phage <400> 9 gtgcccgact ctctgctggg ccaagaaacc gtgagcatgc tagcgtggta cgctgcgtac 60 ttcaacgcat tccccgacaa ggactacgtg gataccgacg tactgcgctc gctgttcacc 120 ttgaaggtgg gcagcaccgc cacgccagag cagctggccg tgatgcgagc gctcatgaac 180 aaactcgacg agccagtcga tcccgaggta atcgacggca tcaccagtca gctgtacgag 240 cgcgacttcg ccggccgtgc cgccgccctg ctgaaccagt acgagaacgg tggcgaggtg 300 gacatcacgt acgagctgaa tcgcatggcc agcgagaaca tgcgccggct cagtgcggca 360 gccccgagtt cgtacattga caccccgatc gaggatatcc tcgcagactt cgggcaggac 420 cgcgggctga agttgcctac cacagccctg aaggagacca ttgcaggcct tcagggcggg 480 gacagtgtgg ctattgcggg gcgcccggac aagggcaaga cgagcttcct agcggcctct 540 ctgactggtt tcgctaagca gctgaagggg ctcggtctgg acggacgccc gctgttgtgg 600 ctgaacaacg agggctcggg caagcggatc atcccgcgaa tctaccaagc ggcgctgaac 660 atcacgttcg agcagatgct gcagatgtcg aacgccggca cgctgcgcga tgcgtaccag 720 aaggcggtgg atgggcagga gattcgcgtg aaggattgcc acggcagcac gctcggacag 780 cttgagcaga tcatcgaaga gatgaacccg tgcgctgtgg tattcgacat gctcgggaac 840 ttccgcatgc ctagcacggg cggcgggaac aagaccgacg cactggagca gatgtggcag 900 gaggtgcggg agatggctgt gcgccacgac ttcgtggcca tgccaaccat ccagatcagt 960 gccgacggcg acaacatgct gttcccgccg tacagcgcac tcaaggatag caagaccggc 1020 gtgcagggcg cgtgcgatgt gatcctgatg atgggcgcat tcaacagtgt ggacttggac 1080 aacgtgcggg gattcagcac gcccaagaac aagcgccaga tgccgggcaa accaagcaac 1140 gtacagagtc aggtgttctt cgatgcaccg acttgccaat tcctagatgg agaatag 1197 <210> 10 <211> 963 <212> DNA <213> Pseudomonas putida phage <400> 10 atgggcgcca agagagagcg cgtgctgcca gtggactgcc accgaccggt gaactggaac 60 gaggcagcag tgcagcgcgt cattgataac gtcgggtatg tgtacgccca gaccaagaag 120 gatggcatcc gattccatgc catgatcgat gctaacgatc gggtgcgtgt gctgacccgt 180 gagggcatcg agatactgtc ccttgacctg cacaaggaac gcctccgtgg gctcctgcaa 240 gccctgccca agcagtttgc gctggatggt gaggcggtag taccgggtat cccgttcgaa 300 gaagccagcg gcatcctacg gcgccacaag gccatcccgc tggaggactc cgtagagttc 360 tgggtatggg acgcgctgcc cgttacggaa ctgttggggt acgaggccgg gactgcaaca 420 ctggacgagc gtatcgacac gctggtgcag gcattcggca gcacgccgga gccgcaggcg 480 cagatcgtgc acatggaacc ggtgagcagc atggaggaac tgtacctgct gttcgatgcc 540 caacgtgagc tgggcgagga gggtctggtg gtcaaggacc cgagccttgc accgcgcaat 600 ggcaaggtca gcggcgcatg gaagctcaag ccgagcgaca cctgtgacgg ccgcatcacc 660 ggtgtgctct ggggtacgcc cgggctgggc aatgccggca agatcatcgg cttcaccgtt 720 gaactggaga acgggaacct atgcgatgtg gatggcctta cccaagcagc catggctgaa 780 tacaccgttc aagttgagaa ctgggcgcac tgtcaatccc caccagaata cgtatcgccg 840 cacccattcg ttgggcggta cgtagaggta agctacatgg agatgaccgc caagggcagc 900 ctgcgacacc cgaagttcaa gaccttccgg gatctggatt attgccccgg atggaaatgt 960 taa 963 <210> 11 <211> 2355 <212> DNA <213> Pseudomonas putida phage <400> 11 atgtacaaga tattcatcac tgacttagag actgagaaca agccttggta cggtcagata 60 gcatcaccat tcaaccctga gaactacatt gtagcccctg ggtggcgagt ggacaccgtg 120 catgacgatg gctctgtcga ggtaggtgag acgaagtgga ggtacttcgc atccgctgag 180 gaggcagacg ccggggccga ctggttcgac gagctggagg ggtgcgccat catggtggca 240 cacaacagca tgttcgagca gaagtggttc ctgagcaagt accgggagcg cttcgaggcg 300 ttcctgaagg ccggcggtag gctagcatgt acccaaatgg ctgagtacct aatcacggct 360 cagcaggaac tgtacccatc cttggacgag actgccgtga agtacggcgg tacgcacaag 420 gtagacgggg taaagattct gtgggagcag ggctacctca ccagccagat cgacaaggac 480 ttgctgatcg aatacctcgc cggcccgtcc ggcgatatcg acaacactgc gctcacgttc 540 tacggccagc aggagaagct ggcagagatg ggcatgaccg acatgtactg ggagcgctgc 600 gactctaagc tggcattcgg ttactgtgag tggttcggcc tgcacgttga tccagaggta 660 gctgagcgca accgcgcagc acaggaggcg gagatcgcag agatgcgggc gaagctgaac 720 gagctgctgc ccaaggacat gcccgactgg atcgagttca gttggacgag ccggtacgat 780 gtgtcggccc tgatctatgg gggcccggtg aagggtcgct tcaaggggcc gtacgacccg 840 ccccagtacg tcaaggccga ctacgtgaag actacggacg gtgcaacctt cctgttggag 900 gagatggata cggtcacgct ggacgccggc gcggactggg agcggtacaa gtcgggcaag 960 aacaagggtc aggtcaaggt gttccgcgag gacaccgacg aggagaagct gaagtggcac 1020 gagggttcgt accttatgcc cgggctcgta gacctgaagt ccctgcccga tgtgatcagc 1080 agcaagtacc ttggcaagcg cgccgagttc cgcggtgccc agaccctgtg cgatggcgtg 1140 accccggtat acagcaccgc tgaggaagcg ctgaagggct tgaagaacta cgtgcctgag 1200 gtcaagatca tggttgatct ggccaaactg gagaaggata ccggtacgta ctatctgcgc 1260 acggagtacg atgctgatgg gaatgtcaag gaagtcaagg gcatgctcca gtacgttcag 1320 aaggaggaca acatcgtcca ccacagcctg aacaccgagg cgaccgtgac cacccggctc 1380 agcagctccc gcccgaacct gcagaacctg ccacgagcag acgaggacgc agacggggag 1440 gcgaagtccc gcgtgaagga aatgttcacc agccggttcg gcccgcaggg caggatcatc 1500 gaggtggact acagtgcgct ggaagtcgtg atgctgtgcg ccatgaccaa ggacatggac 1560 ctgctgaagc tcctgcaggc tggtaccgac atgcactgct accgtttggc gttccagctg 1620 ggcgagccat acgaggaggt gctggccaag gtcaaggatg agagtcaccc tgagcacggg 1680 aagtacaagg ccctgcgctc gaacatcaag ccgctgagct ttgctgacca gtacggcgca 1740 tcagcagcgg ggctggcgtt caacactggc tgctctctgg agttcgctga gcagttccaa 1800 gagaacgagg cgaagctgtt cccgatcagc cgtgggtacc gtcaggtgat catcgacgag 1860 gtgaaccgca ccggcagcct gccgggcagc atccaccgcg agatgtacga caacggccag 1920 tggggcgtgt accgtcgcgg gtacttcacc gggccgagca ccactcggta cagcttccgc 1980 cagcagcgca cttgggacaa ggagctgcgg caggaggtga tggcgtacaa gccaacccag 2040 atggcgaact acccgttcca aggcgaggcc gggttcatga tgtcgtccag catgggccgc 2100 atctgccgct ggctgatctc cgtggactgg tttgacaatc aggtatgctt gatcaacaac 2160 gtgcacgacg cagcgtatct cgacgcagca aacgaggagg taggccgggt ggcagcgctt 2220 ggcaccaaga gaatcatgga ggacgcacca cgctatctca ccgagatgtg gccggcgttc 2280 gacatggccg atgtaccctt cccggcggct gctgagatgg gcgcgaacat gatggagaaa 2340 taccacgtcg agtga 2355 <210> 12 <211> 1005 <212> DNA <213> Pseudomonas putida phage <400> 12 atgagcgacc tgaccagcct gctggaaggt ctgccatccc agttcgagcg ttccgtctcc 60 ggacggacgc tcattctgga tgctgacttc cccgcgtatg aggcctcggc cacggtgaag 120 aagttgccca ccgccctgac ccgcttccag acgctggtgg aaaccgaggt gttcctgacc 180 agctccatgg atgtgcaggt gcacctgacc ccgcgtggct gcacgaagtg ccggcgatac 240 gactacccca cggccaagcc ttaccaaggc aaccgtgaag gcaaggctaa gccgccgctg 300 ctggagccgc tgcgcatggc catcccgagc cacccttggc ctgagcactg gtctacgttc 360 gcatggcgtg accgcgaggc cgacgacggc atgatgatgc acagcttcgc cctcggggca 420 aacggactgg tctgctccgg cgacaaggac ctgtgcctga ccccacatcc gtactggatc 480 atcgacgagg gccgggtgga catcatcaaa gaccggttcg gctggatcaa gcgcaaggag 540 tgggacaagg actggaagat cgtcggccac ggcacgaagt tcttctggtg ccagatgctg 600 atgggtgacc cagctgacaa cgtgcagggt atcctgcgct ggtacggaaa gaagtgcggg 660 aagcagggtg catgggagat catgcacgag atcgacaacg aggatgaggc ggccaacctt 720 gtgctgcagg cgtatgccga cgcagggcag gacttcttgg ccgaggccca gtgcttgtgg 780 ctgcgccggt cgctggacga ctgcgcattc aagtacctga gtgagctgga ccttaagccc 840 caactgcgcg agtggatggg catgctgcac gactaccacg gccaatactt ggccgctatt 900 gacgaacaga aggaattcga acgtggcgaa gatcaatcaa gcgaaggctc gggaactgaa 960 gcatcaggga accccagcgg catccgtctc ccgtgggaag actag 1005 <210> 13 <211> 378 <212> DNA <213> Pseudomonas putida phage <400> 13 atgctgcaag agcaaggtgg cgtgtgcgcc ctgtgtggca agccgatcga cctgagcatc 60 aagggtgaga tggtcattga ccacgaccac gagacaggcc ttgtccgcgg tgccctgcac 120 cgctcctgca acgcagccga gggcaaggtg gccaacgccg ctggacgctg gggtgccaag 180 agcatgtcgt acacggcgat cctgccgtgg ctgacgaacc tgctggcgta cctgcagaag 240 accccacagc cttacatcta ccccacacac aagacggacg aggagaagcg ccttgaggct 300 aacgccaagg cccgccgcgc ccgcgccacc agacaggcac gccaagtcgt gcgcaaatcc 360 gtaatcaaga aggactga 378 <210> 14 <211> 963 <212> DNA <213> Pseudomonas putida phage <400> 14 atgcgcctcc tgaaacttct gacgaaagac cagcatatcg aagtgctgac ccgccatgcc 60 tgcaacgtag aggcagcgca ggtatacagc atcgccatgg gccagccggt gacccgccag 120 aacgtggcgt actggcgccg catcttcatc gacgagggca ccaaggccaa ggctgaccga 180 ctgctggccg ccggccgagc actgcgcaaa gctgagccgg gcgacgacgt aggcgacatc 240 tcgttcgtgc cgaaggtggc ccaccgcatc ctcgtgatcg gagacctgca tgagccgtac 300 gtccatcctg atgcctacga cttcctcgaa cacctgaagg ccgagtacaa tccggacctc 360 gctgtgcaga tcggggacga gacagacggc catgccctga gcttccatga gtcagacccg 420 aacctcgaca gtgccggcgt ggagctggag aaggccaagg agggcctggc ccgtctgtac 480 gacctgttcc cgcagatgct ggtgtgccac tcgaaccacg gctcgctggt gtaccgccgc 540 gcgaagtaca gcggcatccc aatccagatg atcaagtcgt accgcgagat cctgttcccc 600 gggcacagcg cctgtggctg gcactgggcc ttcaactgga agatcgagac cccgctgggt 660 ccagtcctgt tccgtcacca gaccaccggt gatgttatgg acgccgccgc cgctgagggc 720 tgcaacctcg ttgtgggcca cgagcacggt aagttcggct cccgctgggg tgccaccagc 780 atgcgcctgt tctatggtgc atacaccggc tgcctgatcg acaaggacag catggccttc 840 gcgtacggca agacgtaccc gcgcaagcca atcctcggtg ccctcctgat tatcgagggc 900 gttcctgtta acgtgccgat gctgctcgac agcgacggcc gctgggtagg gccacagcaa 960 tga 963 <210> 15 <211> 270 <212> DNA <213> Pseudomonas putida phage <400> 15 gtgatcgccg ccctagcggt gttcaccgca gcgtacgcca ccgtaatgct gctggggctg 60 cagtccaagc tcatgcgcga caacaactgg cgcctgagct tcttcaccag ctggttcatc 120 acgttcgccc aaacgggcag cacgtacgcc atcgcccaca acgccctgcc catcgagtgg 180 ttcctgttct tcagcggcct cggcggcagc accggcattg tgtcggcaca cttcgtttac 240 gattggtacg acaagaggtt taaacgataa 270 <210> 16 <211> 2436 <212> DNA <213> Pseudomonas putida phage <400> 16 atggacctga ttcaacggca aatcaacatc gagcaagagt acaccgccca aggcattgcg 60 ctggcaatca agaagtggga gacggacatt gccgagggcc gtatcgcgga cactggtgtg 120 ggtaagcgca tggtgatacg tgcgctagcc ggtgttcgct ccgaactgga tagcatctgt 180 aatgccggta cacgcggcgt gggcggccgc tatcgcagcc tgctacgtga tatcgggctg 240 gacattgcag cggtcatcgg cctgcgcgcc ggcctcggct tagtcaccgg ccagcgtgcc 300 cgtaacggcg agccccttgt gcaggacctc cttagcgaag taggcgcaca cctagagatc 360 gagcatatgg cggtcaagct gaaggcagct gccccagcat acctaggaag ggtcttggac 420 agcctgcagg aggcccgtac gcgcagttct aaccacagac gacgtaccct acaggctacc 480 gccgataacg tctctgtggg ctctgagaac gtcacatgga gcaccgggga gcgagagggt 540 gccgctaagc tggtgctgga ggcgctggtt aatggcgggg tgctggacat gtaccacatc 600 cccaaaagcc gtggccagag ctgggtggcc ctcaaagcca gcgatgccat tgctgcgcac 660 gtagatcgca tgggcggtgc ggtgcgggca ttcatgctgt ttccgccaat gcttgtgccg 720 ccgaagccgc atacccgcga cacactgttc tctggggcgt catacctcac agagggtatg 780 tcaatcaact cggcaagtat tcgagtacgc tcccggcgtg cagaccatcg acagtggatc 840 aaggacaatg taagtgatac ggtcctaact gccgcgaacg tggccgcatc tcagccgtac 900 tgcatcgatg tggagacggc ctcgatcctg cgcgacctgt tctcccaagg tgtttacaac 960 ggcatcgcgg gcataccgtc acgtgatccc atccagccgc cggcgtatcc gctcaaggag 1020 aactgggacc gcgaggaccc tgctctacag gaggagcacg ctgcgtggaa ggtgctggct 1080 cggcaggcct acgccgacga gctggcccgg aagtcgcacg ttgtgtcgtt cttgcagacc 1140 ctgaagtatt tgaaggagta cgcaggagat accctgtatt tcccaacata cttcgactgg 1200 cgcggccgtc tgtacttccg cagccggatc aacccgcaag gcacagactt tgtgaaggct 1260 gtgatccagt ttgcgaacaa gaagccgctg ggtaaacgtg ggctgtactg gctgaaggtg 1320 catgtggcca cctgctacgg cttcgacaag aagctgccag actgccgggc ggtgtgggtg 1380 gacgagcaca tggcgcagat caaggacgct gtggcccgac acatcgactc cgacttcttc 1440 cgtgaggccg acagcccgtg gtgcttcttc gtggccgcac gggagatgct gcgcgccatc 1500 gaatccggcg accccgagtc gttcgagaca ggcgtgcccg tcgccatgga tgctacttgc 1560 agcggtatgc agcacctatc ggcagtgctc cgcgatcccg tgggtggtat gttcacgaac 1620 ctgctgttca ataacgggac cgagaaggaa gacatctacg ccggtgttgc agcaattgct 1680 atatcccgac tgcaggctga ccgggacaat cctgagcagg ccgcgtactg gctgacccac 1740 ggggtgcccc gcagcatggc aaagcgccca gtaatgacgt acgtgtacgg cggcaccctg 1800 atgtcctgcg gggattacgt gctgctggac atgcaggagc gcgggctgga ggccttgccg 1860 gagtacagca tgtcgaagct ggccgtgtac ttgggccggc atctgcggca cggtatcgag 1920 ctggcggtgc cctccgccgc gtctgctatg cggttcctgc gtgcgcttgc cggcatgatg 1980 ccccgcgatg agcccatgcg ctgggtatca ccagcgggat tccctgtgat ccagcattac 2040 gcggccgagg aggttgtgcg gtttgacctg caagggttgg gggtcatggt gagcatgacg 2100 cgctttgacg ataaccgact gaatcgctcc aagtgtgtga acggcatcgc cccgaacttc 2160 gtgcacagcc ttgacagcgg gcacttggtg cgtgtgctgg ccgcgtacaa gggcagcctt 2220 gtgcctatcc acgattcgtt tgccacgcac ccatcggatg tggatgacat gcactgggtg 2280 ctgcgcgatg agttcgtgaa gatgtacgag gagtcggacc cgatggacgc gttggtgcag 2340 ggtgtgcagc aattccgcga agaggtgatc gagcagcctg taaggggcac actagacatc 2400 tcgaaggttc gggattctgt gttcttcatg tgttag 2436 <210> 17 <211> 1515 <212> DNA <213> Pseudomonas putida phage <400> 17 atgaaggcac cagtcacata cgagagcttg ttcaccaagt accgtgacga cagcgccgta 60 ctgcggaacg agcagtatgc gctctggagc atccccacga tctactcgga ctcagatgtc 120 cgcacggacg ggaagcagca gagcgtacgc cgcgacttcc agtccgctgg cgccatgctg 180 gccaaccact tggcgagcaa gatcgcatcg ctgctgttcc cgtccactca gtcgttcttc 240 cgccttgatt cggcagtgaa ccctgacgag atggcctcgg cactggccgt ggaccagaac 300 gagctggcct ctgggctggc cacgctggag aacactgcgt accgccgcat cttcctccgc 360 tcgtcgtacc atcagctcgt gcatgccatg aagctgctga tctgcacggg gaactgcctg 420 ctgtaccgcg atagcgcgaa cacgaatatg cacgcctaca gcatgcgctc gtacgccatc 480 ctgcgggatg ggtcgggcaa ggtgctggat atcatcctga aggagcgcac cacagtcggg 540 cagctgcctg aactcctgca gagcacggcg tatgccgggc gcgaggagta cgatagcgtg 600 tgcctgtaca cccgcatcaa gcgtgagcgc cgcaaagtca ccgacgtatt cgtggtgacc 660 cagcaggcgg actcgcatgc gctggatgtg cgcgaggagt acccggaggc agtatgcccg 720 tacatccccg taacgtggaa cctcatcacc ggtgagaact acggccgtgg cctcgtggag 780 gactacgccg gcgacttcgc caagctgagc gagctgtccg aggccctggc cctgtacgag 840 attgaggcat gccgcgtgtt gcatatggct gccccgggct ctcaggccga catcgattcg 900 atggcatccg aggagatggg cgcttgggta gcgggtgacc ccaccaaggt gcaagcgtac 960 gaggctggcg actacaacaa gattgtggcc ctgaccgcgg acctgacgca aatcttccaa 1020 cgacttgcgc cggcgttcat gtacggcggg aacgtgcgag atgccgagcg ggtaaccgct 1080 gaggagatcc gtacgcaggc cgaggaagcc aaccagtcgc tgggtggtgt gtacagcgtg 1140 atcgctgata acctgcacat cccactggcc cacatcctgt gtgccgaggt gaaccctgac 1200 ttcgttgccg aggtaatcgc cggcggcctg accttgagtg tactcaccgg cgtcgccgcc 1260 ttgggtcgca gctccgatgt gaataaactc ctgcaggttg cacaggtgtt gggtgccacc 1320 ctcccagtat tcactcaggt gagccagcgg ctcgaccctg agcgcgtcat ctccaaggtg 1380 ttcgaaggct ttggcctgaa catcgaagac tattcccata ccgcagaaga gttggaagcc 1440 aaacaggccg ctaacgctgc acccgtggac actgccctga ccggtgccgc agctgacatc 1500 caaggagtcc tgtaa 1515 <210> 18 <211> 834 <212> DNA <213> Pseudomonas putida phage <400> 18 atgtctgaga accagaccca agatcaagta aacgagttcg gcagcaagat cagccccaac 60 gctgacgcga atgttgccct gaacgagaac ggccctgatc agggtactct tgctcaaatc 120 cttgcagcca tccgtggtaa ggacgttgag acgcagccag ctgccgccga caagggcaac 180 ctcggtgttg atgctgatgc cgacgagaag ccactgccaa ccgctgacga cgaggacttc 240 aagttcgaga ccggcaacaa ggcgctggat gtggctgtat cctcgttcct tcgcagcacc 300 ggtgcaagcg atgccgacat cgagcgcgca tgcaagtccg ctctggagta tggtgaccct 360 aaactgatcg accgcgcctt cattgctgag aagttcggtg accgcgcagc ggacgccgtg 420 gctctggccg aggccgtaat cgagcaggcc ggggttgaga aacaacgtac cgtccaagct 480 gtgtacgatc tcgctggctc gaaggagcag tgggacaccg cactgggtgt gtacaagcag 540 cacgcccccg ctggcctgca gaaggcgctg gggattatgt tcagctcggg agacgccgca 600 tccgtgaagg aagcagccgg tctcgtgttg gagtttgcca aaggcagtgg agttctcccc 660 gtgggtgcgg ggcaacgcca agtggctgga ggcggtaccg ctgcctctga aggcctctcg 720 gctcaagaat tcaaggccgc actgagtaag ctgaacccga acgcacgcag ctaccatcag 780 gactatgagc gcctgttgaa catgcgtcgt gtcggcaagc agctcggcat gtaa 834 <210> 19 <211> 990 <212> DNA <213> Pseudomonas putida phage <400> 19 atggctaaca ccgcctacac tggcaacctg actcgcccgc actggggtgg tccgaactcc 60 gacgctgaca tccatctgga ggtcttcgac ggtgccctcg acaccgcgtt ccagtacaac 120 gcgttcttcc gtgccaacag caccttcatc tcggtgaacg atcgttcgaa caccgcccgc 180 atcgaccgtc tgggcactgt gaccatcaag ggtcgtaagt ccggcgaggc gctcgatcct 240 actccggtca agaacgacaa gctggtaatc accgtggaca ccgtgaccta cgcacggtcc 300 ccgatcgatt accaagacga ctggaccgct ccggacttcc tgccggctat cggccagaac 360 cacggtaccg agcacgccaa gctgttcgac caagcgcaca tcatccagct gatcaagtcc 420 cgctcgtgga ttgccccggc tcacctgaag ccagccttct acgacggctt cgagaagacc 480 gcccagctgg tgaccaacga cgctgctgcc tcgtctgacg ccatcgtcgc tgcccacctg 540 agtggcatcg agaccctgat caaccgtgat ctgggtggct cgctgaacga gttcatcact 600 ctggtgaccc cacgcgtgtt ctcgctgctc ctgcagaacg agaaactggt gaacgtccag 660 tacagcgacg gtaacgcaaa ctacgcccag cgccgtgttg cgtacctgaa cggtacccgt 720 ctggtcgaga ccgctcgctt cccgaacgct gtcatcacca accaccagtt gggcgctgcc 780 ttcaacgtcg actccaccga catccaagta gagatgatcg tgtatcatcc gaagatgact 840 ctggtgaccg ttgaggccaa ggcgcacacc tcccgcgtgt gggacgatga gctgaacttt 900 gcgaacgtgc tcgactcgta cgccatgtac accatcggcc aacgccgccc agacactacc 960 ttctcggtac gtctgctgcc tggcgcgtaa 990 <210> 20 <211> 555 <212> DNA <213> Pseudomonas putida phage <400> 20 atgacacttt tagaggcggt aaacacctgt ctgtccgccc ttggcgaatc gcgggtcacc 60 agcaccacag tagtgcaccc tacggtggac ctcgtcctga gcaccatcgc catgaagcag 120 cgccagctgc tggagcgcgg gtggtggttc aacatgagcc acgtcaccat gtacccatcc 180 accacgggtg agatggagta ccccgccaac gccattgcca tcgtcggtga tgagggcgaa 240 atcttcgtgc cgcgcggcgg catgctgttc gatatggaca acaacaccca gtatttcacg 300 gagcccaaga gcatgaccgt ggcgtacaac ctcgacttcg aggaccttcc cgagtgtgct 360 gcaaacgtcg ttatgacccg cgcggcgcag gaagtgtatt cgggtgacct tggcccggac 420 gctgctgtgc agcgcctgca gggccttgag atcatgagct ttgccgagat ggagttgcag 480 cacatgcgca accgccggta cagcaccaaa caacgtcgcg gatggggtcg tctcgtccgt 540 gccctgcgcg gctag 555 <210> 21 <211> 2334 <212> DNA <213> Pseudomonas putida phage <400> 21 atggcggcta tcgacggcgc aaccaaatcg ttaatgcagg gcgtgtccca gcaagtaccc 60 cgcgaacgtc tcgacggcca ggtctccctt caggtgaaca tgctgtcgga cgccgttgaa 120 ggcatgcgcc gccggcccgg tatgcggtac ctgaccagca acatcttccc gggcggcgct 180 tggaacaaag agcaggtgtt cgccacctcc gtggacattg gggacgagcc ccaccacatc 240 ctgatcaaca ccgccaccgg cgttgtgaac gtgtacaaca gcaatatgtc gctggtggct 300 actgacgact tcttctacct gctggcgcct accgctgcca gtattcaggt agcggcatta 360 cgtgggtacc tgtacatctg caacactgag cagttcccag tcaagaacgt gactaatgcc 420 ggcaagcaga accctgacaa gacgggtttc ttctttgtga agacggggca gtacagcaag 480 atttacaacg tcactctgaa cgtgaacggg gtgcagtacg cctcatcgta cactacaccg 540 aacggatccg cccccggcga tgccgccctg agcacgcctg agtacgtagc tggcaagctg 600 gtggacggta ttgtggcaca cgccattccg ggcctctcct ttgccatcgc gggctcgtac 660 gtgtaccttg tcaccacggg tgctggggcc atcactgtga cctcagattc cggcacaaac 720 ttcatcagcg cctcgaacca gtcccatgtc accctgacta ctgagctccc tgcccggatg 780 cccgcagctg ccaacggcat gctggtaagc gtgggcgcca acccatcgct atccgtgtgg 840 taccgctttg acttcgtgaa caacgcgtgg gttgagtcgg gggcttggaa cagcgccgac 900 ttcctgacga atatgcccat ccggttgaag ctggataact cctacgtgat ggaccagcca 960 aactatgagg ggcgtttgtc gggtaacgac aacacgaacg aggaccccgc cttccttacg 1020 gacggcgtga ccggtctgag cgtattccaa ggccgtctgg taatcctaag cggggcaacg 1080 gtgtgtatga gcgctgcggg caaacccctg cgctggtacc gtagcaccac tacggaactc 1140 ctcgttgccg atcccatcag catcttctca ggctcggcta ccactgccaa cttcacacac 1200 gccatccagt ttaacaagga cttgttactg tttgccaaga cctgccaagc ggtagtgccc 1260 tcgggtaact ctgtagtggc gcccagtacg gcccagatcg tcatcaccag tgcgtacaac 1320 acaactagca aggtgctgcc tatcgtggca gggcggtccc taatgtactt cgcaccccgc 1380 agcgagaagt tcgccagcgt cctagagatg gtgccgagta acaccacgga ctcgcaatac 1440 accaccaacg acgtaactgc acacatccca cagtacatgc cggggaccat tcggcaggct 1500 acggctagca ccaccagcaa cagccttgtg cttgtcgctg acggggatgc ccgcaccctg 1560 tttgtgcacg agtacttgtg gggtggggat gagaaactgc agtctgcatg gcataactgg 1620 accgcaccgt atgacatcgt gtgtacttgg tttgtccgcg acacaatcta cgtcggtatg 1680 ttagtagacg gcgacctcgc aattgtcacg atcgactcac aggcaggcga cacagccaac 1740 ggcctgtacc gcccgttctc ggacatgttc atcggcactg cagtcgtcgg cggcaccttt 1800 acggttccca cggcgctgcg cgctgcatac acagccggag ccgagttgca gttgacctac 1860 accaccggcg cagttgctgg cgaggcagta ggcttcagca tcaacacagc cacttgggtt 1920 ggtacagcgg tgcgtaacgt ccctgacggc acgtacttcc taggcctcaa gtaccgcagc 1980 acgctgtcac ctacaccacc gctactgcgc gatcagaacg gtgtggtgat tggaaccgca 2040 ggcacatccc tgatccggta cgaggcctcc ctgaagaaca cgggtgattt ccatgtggtt 2100 gtgcaacggc acgacgagac tacctcggaa ggggtatact ccggccttac atacagctcg 2160 caggacttga tccccggtgg gcccctgtac gccaagagta gccggtgcat catcccagtg 2220 cgtttgaacc ccgacgcctc tagcgtgatc atgtacgcag aagacgatca cgacctcggc 2280 gtgcttaccc ttgagcacgt tatgcagtac caccctcgac gacggagaat gtaa 2334 <110> Industry-Academic Cooperation Foundation, Yonsei University <120> Lytic bacteriophage specific for Pseudomonas genus resistant to antibiotics <130> DPB155798 <160> 21 <170> Kopatentin 2.0 <210> 1 <211> 45417 <212> DNA <213> Pseudomonas putida phage <400> 1 tgcacaagcg gccaaatcct tcaatgagat tgctctgcag aagactgccc tgtccgccca 60 gatcgcaact gcccgcctag cggtgcagcg cgagggcgag caggtgaagt ctgcgcgggg 120 gctacaggcg gcgggcacgg acaccatggg tgcatccgta gcccaaggcc tgctggatgt 180 ggacatgaag atttccgagg cgcagcagga gctgctgtac aacggcaccc ttacggatca 240 gtcgttgaat gctcaggcgg ccaatgtggc tgacagcgcc ggcatgtccc ttcgcacccc 300 cactgccgtg agcaacaagt gggcacccat ccttgggcag gccgtggcca gcttcgggat 360 gtccctgttt gagaacaaag cccgcaccgg tagcttcacc gggcggaacc aaggtctata 420 aggaggcatc atgccagtac gccaagatgt acaaggcggc ccgcgggctg ccggtcttga 480 gggttatcag agcccgggag ccgcccgtgc gcagttccaa ggcccagacc tcagcggcgt 540 acagcgcgat gccgcagacc aagccaactt ctggacccgg ttgatcggcc gtggggcgaa 600 cgccctgcag gctatggacg agaaagaccg ggcccgggcc tatttagagg gtcagcaaga 660 cagcgccatg ggcaaggccc gcgctgaagt gtatggctca atgcagtccg actacgagca 720 aggctataac cgcgccacca ttggcagcca gttggcgaag taccagctgg gcgtgcagca 780 agccgccatc gaacatgtga actcgggcaa gaccccagag gagttccagc agttcgtgga 840 ggaggagacg aactccctgc tgaacgcagc cggcactcaa ggccttgatc tgaacgagaa 900 ggactggcag gcgtggctga ccggcgtgca gagcacccgg gccaccgccg ctgatctgtt 960 ccagaccaag agcgtagagc gtgcccagta catgaagggg caggccatcg ccgccgaggg 1020 caacgccagc attgctacct tcatggctgc cgatgaggcg ggtaacccac tgcaggcgct 1080 gggcgggatc acctcccaca ttgcccgcgt gtactccgac aacaccttgt cgcctcagca 1140 aaaggacggg gcccttgcgg acttcgctgg ccaactcatg gcaagcgcta agtcgtccgc 1200 cgccgttgag gcgctgggct cgtacttcca agagtcggcc gagttcaaat ccctgcctac 1260 tcaggtacag acgcaggtta tcaacggcgc ccagcgggcg tacgagcagc gtgcagccga 1320 cgagtcgggt gcggtgtact cgtacgtgtc gaacgtgcgc gctgtggcca acccggatga 1380 gctggagcgc cagttcccca tggcgtcctt tattagcacc ctgaacgagg cccagcgggc 1440 tcaccgcatc actccggccc agatgtacgg tatggtggag gaggagaaca ctcgccgcct 1500 gaagctcggc aaggctgccg cattccagca gaacctgctg cacggcacca ccatgtccga 1560 tatggcagct ggtaccggtc agactctcgg caagatcaag actgccctca cgcaggtata 1620 cgccaccagc aacggtggct acagcggcgg cgggctggcg ctcatgcagc gtggtctgca 1680 gagtggggcg caggacatca cagctgtggg tatcgagatg ctgcagcaag acgcacagtc 1740 cctcggcagc atcgacagcc ggaacctgaa gcgcgatgct gacgggcagg ccctgtaccc 1800 ggctaccgtg gtgaactcgc tgacgaacct gaagcgtgcg tacgacgctg cacaacgagc 1860 cggcaacaac gtgcaggctt ctcagctcat tgctgggctg cccgacgctg tagcctatgg 1920 catccgccaa gccagcgacg agaatagcat tgcggacgtt gtgtaccgcc gggccgatga 1980 tatcgcctcc gggcgcgtgg tctccctgcc ggctgccatg cccaaggaaa tgctcgccac 2040 tgtggacgat gtatccgccg gcatgttcga cacgaacctg acccagaagg gcgcggctcg 2100 gaacatcctc ggcatccagt cgtacgtgtt caccagcaag gaagatgccc gggcgcagga 2160 agcccgcttg gcgcaggtga acggtgccct cggtgaggag tacacctcac tgtaccagca 2220 aggccgtctg ccagcgctcg ccggcgacga cctgaagaac tggctggtgg gtcgtgtggc 2280 tgcccgtacc gtgcgtgtag acgatggcac cgacaacggc acactgctga tcctgcctaa 2340 cgtggccaac cgcgcccaga cctttggcag tgaggacaac aacatcatcg gccaaggcct 2400 gaaggactct gttgacgagt tccgtgcagc caatcccggc gctgtatccg tccagatgcg 2460 gtacgacccc atggctggcg agatcgtgtt cagcggcact gacaaggaca acacggtgct 2520 caccacttcg tacgccgtgc cggtcgatca ggttgtacgc caagtgcgtg cagttgagac 2580 tcgcctgacc gcgaacggta atggtaacag catgggctcg ctcgctgtgc ccggcgccgg 2640 gtttgtgcag tttaatacgg ccaacgccta cggaatcgag ccgcaagtgt atcaaaatgc 2700 agttacccag ctggtctcat acgagggcta caccgacacc aaaggcttca gcatcctggc 2760 cacccaccca accacgggtg cggccctgaa cgaggccaag tacgtgaagc agcccaccga 2820 cacgccacag gtcgctgcga acaagctgca actgtacatc caagacaagg tgcttccgcc 2880 tgtcatggct gagttgccgc agttcaacgc cctgcctgag cacatccgtc agcaggtgtt 2940 ccagcagctg gtggagacga cgtatcacgc cggtaacgcc aaggccttcg gggacatcct 3000 gaagaaggcg ctggatggtg acacactggg ggcctaccaa gacttccgcg agtccgccct 3060 gtacaaggac gctgggccta agtcccgccg caaccgtgac cgactgcaaa cccttgacgc 3120 tgtatcccaa tacagccgcg tgcaacgcac cggctactga actggaggtt ccaatgagca 3180 tgatccgttt acgccctgac gaagaccttg ctgtcgcacc gggcaccgcc ccgactgtcg 3240 gcggcgggtt cgtctccggc gctgagaaca gcttactgga acccggtaca gcaaacacct 3300 cggcgctgaa agtagcgccg acctcttctg cagagagtgc cgagggcgct ctccgcgaaa 3360 ccatcgcagc caaggcgctg cgtggtatcg agtacatgca aatcccaagc gaggaggggt 3420 tcgaccctgc agccgcgctg ggcgacaaac tcggcgcctt cactgaggaa gagctggagt 3480 tccttggcga tgcccgctcc agcaccgaac tggcccagcg cctcggtcaa gtgacccaga 3540 cccgccagaa cctgcaggac atggccgcca accctgtaac cgccttcgcc gcctcgatgc 3600 tggacatcga cgctgtgatc ggcctcgggg tagctggtgc tgctacgcgc gcgggccgta 3660 ccgcgcgtct ggttgccggc ctggccgcta acactgcggt actgggcgct gcatccgagg 3720 gcggtgagat caccccgctg gatgtcatcg gcacatcgct ggtcgtagcg ctgggcgcaa 3780 tccccaccgt acgccgtgca gcaaccgttg ccgaggacgc ggctcaggac ctcccaacag 3840 gcgcgcgtag cgttcctgac gttgaggagg tagtaccccc acgccccgct gctgaagtcg 3900 ctccagatgt cgtccctgac gctcaggtgg ccccttcccg catccctgac ccagactatg 3960 aagtccctac ccctgacatg acgactgtac gcccgtacgt cgaagtgaat cgggtcaagg 4020 gtcaagccac catccgcacc agtactgcga accttgtggg cgccacactg gccaatgcca 4080 gcgacctgcc cgagggtacc cgccttctgg gtgtagcctt gtacgatagc atgaagctgg 4140 acggcgacgt gcccctgatc atcacccgtg gcaagggtcg atcgaacgtc cgcctgtggc 4200 cagacggccg cctgcagacc accatgcgca cagccgaggg cggggcacct accctgactg 4260 aggccgtgca gaacatgagc acgtacgaga agaccgttaa cctgcacgag gcagcacacg 4320 ccaagaccat cacagcgatt gccctgttcc aacagggtaa gctctcggct ggtccagtgt 4380 tcgacgcagt gcagcagatc gatcaaatcc gcaacgtcgt gcggggcaac atcagtaccc 4440 caaccctgcg tgagggcatc agcacttccg actggaagaa caacgtgctg tacggactca 4500 gcaacaccca cgagttcgtg tcccagctgt tcaacagcga gtccttccgc aaggtactgc 4560 accagatccc tatgcccggt gagccccgct cagtgtggtc tagcttggtg cagaaggttg 4620 tgaccgcctt cactggcaag ggtcctaccg acaccgcgct gacccgcttg gtggctgcat 4680 tcgaagacct attgcaacag ccctctgtga ctgacgcgca gttcgtgaag gcgtacaaca 4740 aggccgacgt gccgcgtacc caatcggccg cgctggctaa ctccccagac atccagacca 4800 tgttcacccg caccgggaac tcgttgaatc aggccttcgc cttgttcgac aagattcgcg 4860 ccatcggccc acgggccagc acccttgcgg aacaactggt tgtcgatgcc actgggaact 4920 cggctacgtc tgctgcgcat tacgcccgca ctgcccactt ggctgccaac gtggccgctg 4980 cgcaggttga tgctgctgtg cgtcaggccc tgtccgccca aggctggact atgttctctc 5040 gcctgcgcaa cccagctgca ttccgggctg cacagcagga gctgtccggc aaggtgtacg 5100 acgccctgtc cgataaccat gcccgcttca tgtcgggcgg caccgtgaac cctcatccag 5160 acgcagccgt gcagcgcatc gtcgatgcct tcgccagctc ccgctgggct gaggaccagc 5220 tggcccgcat caaggcctct ggcatgaccg gtgccgacac catcgaagcg tctccgtact 5280 acttgccacg ccgccacagc ggtaacaagg tgagcgactt cctccgtgcc aacccgggcg 5340 ttactcgggc ggacattgag gccatgtacg gtgagcagtt caagttaatg tttgcagacc 5400 gcggtatccg cccggacact gcccgcgcgc tgggtcgtca gatgctgcgg aacatggatg 5460 agcgagccgc cggcgtgcaa ggctaccgcc agcacatcgc aggtatgacc aacgacgaca 5520 tcgagttcgc tatgcgcaac gccggtatcg acgaggatca gatcaacatg ttcttgaaca 5580 ctgtgcagaa agcgggcgag caatccaaca ccgtgcggaa cctccgccgg cgcgtggact 5640 ttgacatgac ccgtgagtac gtgaccaaga gcggcgatgt gatcatgccg cagatgttcg 5700 tggacaagga tgtgctgggc ctgatggaag gctacagccg caccatgagt ggacgtattg 5760 ggctggccaa agccggtttc cctgacgtgc gtacgctcgc cggcgcagtc gatgaggctg 5820 ccgctgaggg cgcagaccca cgcgcagcgc gagccacgtt ggacaacact gtgaaccgac 5880 tgctgggcta ccccactggg gaagacgtgc ctgatatcct ccgcagcttt gctgtggcgt 5940 ctggtgcggt gcagctggcc aactctggta tctaccagct ggcagacact gccctgatga 6000 tcaaggagtt cggtattgcc aaggtactgc ggtccctgag ctccacagag tggggccgtg 6060 acgctttgaa gctggcacag gacccaacct acggctctcg cctccgcgac gttctggaag 6120 cacgcaacgt gctctcaggc cggtaccgga cagtgatgac ccacctcgac gacaacaccg 6180 atatcggaaa cctcggcatg ctgcaccaga acatccagca gctgggtcaa gtgacccgct 6240 tcgccaacgg catggagttc gtgcgccgcg ggcagtcgaa gatgatggcc gggctggtag 6300 gtgacaccgt ggatgacgcc atccgtggca acgacgcggc attcgagtcc ctgcagcgct 6360 ttggcatgac gcctgagctg cgtgcccggg ctaaggccgc gatggatgcc gaccctgaca 6420 tgcggatgtg gccagacagc ctgcgcctcg acatggagac tgtggcgcac aacatggctg 6480 acgcccttgt catggagaac cggttgggtg agcttcctgc ttggatgcag ttcagcgccc 6540 tcggcaagtt catcctgccg tacatgaact tcgtggctgg tacttggaac aagatcctgc 6600 gccgcacgta cgccaacgac ggccttgccg gcgtggctat gatgttcgcg tatcaactcc 6660 cgcttacaac cctctcgtca actgtagcgc ttgcgcaggg caacaaggag atcactcctc 6720 agaccctggc cgtgaacgtg ctgacccaaa tgccgctgat gtcgtggctc gggtacgctg 6780 tgaacatgat gactcaaggc ccgacgaaca gcattgctgc tcttgggttg gtcgataagg 6840 catactccgc taccgcgtca ctggtgcgtg gcgagcctga tccagagcaa atcatccgcg 6900 caaccccttt cctcgggatc atccccggta tccgcatcat ggcctcggcc ttgggcgagg 6960 acgaataagg agacacaatg aacgccatcc aaactgaggt atccgacggc agcttagtca 7020 gcgttaacgt cggactggaa tacttcgagc agtcggatat ctcggttttc ttggacttga 7080 gtatcgcgcc acttatcgaa ggcactgact acgcgtgggc caacgccacc cgcattgact 7140 tcctgacagg ccctgtggcc gcaggtgtgg cggtgacatt ggttcgaaac accgagaacg 7200 aggcaatgct gaacatctac gatggcggcg ccccgttcaa ccgattcacc cttgacgaga 7260 acttcttgca gctgctccgg ctgtcgcaag agttctccga gggcttaggc ttgaccggac 7320 tgcggcaggc cttggacatg cacggctacc gcatcttcaa ccttggtaac ccggtgaatg 7380 cacaggatgc taccaccaag gcgtacgtgg acctgctgat ccagaccctt gtggaatcgg 7440 gtaccggccc aatcggggcc gcctcgaatg tcgtgtacat cgaccctgaa ggccagctaa 7500 agaacctgca gcagctgtct ggtctcgatg gtgcggacct gatcggctgg aatggcacgg 7560 gcctgggtcc gttccttgaa gccggctact acgagccgca agggctggac cttaccggtg 7620 ttgtggacga gtctgcggta atcaacgccg cgctagtgaa gtacaagcgc gtgcgtctcc 7680 cggcaggcac cattaaggct aacgtgctcg tgccatccgg ctgtaccctg cggggcgccg 7740 gtaagacccg gctgaaccgt agcaccaagg tatgggccgc cggtggcacc accattatcg 7800 gctctgtagt ggcgtcgaac cagatcaact ggactgtcac cgacatgaac atcgacggct 7860 tcgccggcgg taacgctttt ggtgctcagg gcccatccac agagttcggg tacatcggtc 7920 gagtggcaat gcgcggtagt gaccacaaca tgatccttga gcagaacggc tctgatcttg 7980 caggccgctt cggcggcaat atcctcgtcg aggactgcga tgcgtatgat ggccctaacg 8040 ggtttgctgt gaagatgcgg aacgtgacct tccgccggtg ctacgcgtac gacatcacag 8100 tgcagggctt tgtggctgtg tctgacaaca tcaacggccc ggctatctac tcgcgcgcgc 8160 agaacgtgcg ctttgaggat tgtggcggcg acagctgcaa ccaaggcttt gtggtgtatt 8220 cccgcgactt ctaccacggg aatttccccg gcagctgggt cgccacggtg agtcccgcta 8280 ccgacatcta ctgggacggt acagtcacga acgtgacttc gggccaagta cacatcggtg 8340 acttcagctc ggacggcacc ttcgccattg tgttcaacaa cgagatcgag attggcggcg 8400 ggcactacca gcactccccg ctgtttgggg tgcggttcga ctgggcggca cgtccaaagg 8460 taaccggtgg ccacttctcc gggtgcccga acccgatcgt gcgcggcacg aacgtggtag 8520 acctcaagat tggtgatggg gtgacgcgct tcggtgccat caacccgggc atcctgtctg 8580 agcgcattat cgagagctca aataccagtg ccgtgaacat ccagaatgta cgccaagtgc 8640 tggtgttcca gaacaccgtg ccgaccgtgg ttacatccct cgtcacgcct gataccaacc 8700 acaccattga cgttgtgatt gacgacggcg ttacctcact acagctggcg ggcatcctgc 8760 tcaccggtcg gggcagccgg gcgcgcttcc agtggtcggc agataccagc acttggttgt 8820 gcctgatgag tggtagtgtg ctgccaccgg gcgagattgc ggttccgtac gccttgagca 8880 tgtctgccga ttggcgcgcc ctggcaaact ccatcgccat gaacggtaac atcaacctgc 8940 tggacgtacc tgtggcgaac atcgccccgg gcactgtagc ccacctgcgc atcgtgaacg 9000 ttgccggcgg ctcacgcagt gtgttcaact ggtcaggtgt aacgtggggt gccatcaccc 9060 ccgtggctgc cattgcggcc ggtgccactg tcctcatgac cctgtaccac aatggaagca 9120 cttggttagt caagagtgcc cagacgttct aaggaggaaa catggctaag tcagcaagca 9180 agagcctgct gaacgagctg cacgagatgc tggcgcaagt cttcattgac gacctgcgcc 9240 agtcgcggga ggagggcatt cccctccccg ccgctaacct cggtgtgatt cggcagttct 9300 tgaaggataa cgatgtgagc gcgagtatcg atgctgacga catggcgaag ctgcgtgaca 9360 ccttcgcaga cgagttggcc gagcgccgcg cactgaacaa agcccgattg cagcagacac 9420 tgcaggagga cgatatcaac gagttgcttg ggtaaggagg ttccatgatt cgagcgcaca 9480 cactagcgcg cctgcggctg ctcgtcgagc gcacgaacaa gtggaaggac aacagcaacg 9540 ccatccccgc tgaggagcga gttgatctcg ccctcatgtt cggggccaca ttcccacgtt 9600 tccgggagtt cgctgagcta gggatgaagt tcctcggctt cgagctcacg gagatgcaag 9660 gcgacattgc agactacatg caggatggcc cacgcaacag catggtggcc gctcagcgcg 9720 gtgaggccaa gagcaccctt gcggccctgt tcgctgtgtg gtcgctcatc cgggaccaga 9780 gcatgcgcat cctgattatc tcgggcggcg agaagcaggc ttcggacgta gccatcctga 9840 tcatccgcat gattgagact tggggtttac tgtgctggct ccggccagac aagacccgtg 9900 gcgaccgtac ctcgtacgag agctacgacg tgcactgcga cctgaagccg ctggacaagt 9960 ccgcctccgt ggcgtgcgtg ggcatcaccg cgcagctgca gggtaagcgg gccgacctgc 10020 tcattcccga tgacatcgag accacgaaca acggcttgac cgctaccagc cgcgaactgc 10080 tgctactgcg ctcccgcgac ttcgcggcca tcaacactca cggcaagacc ctgtacctcg 10140 gcacgccgca gccaaggac tccatctaca agaccctcgc tcgacgggga ttcgaggtgc 10200 gtgtatggcc gggccgcgtg cccactgtgg aggaggctga gcgctacggc gacaccctcg 10260 ccccatacgt ggctgccatg ttcgagaccc atgcccgcac tgggttcggg ctggacggca 10320 cccgtggcga ggtcacagac ccgggccggt acactgagga ggacctgcag agcaaggagc 10380 tggactttgg ccccgagggc ttccagctgc agtacatgct cgacacgtac ctcgtcgatg 10440 ccatgcgcac gcgcgtgaag ctctccgatg cgattgtagc atcgttgggc accgatgctg 10500 cccctgatac cctgtactac gccgcaacgc cacagcaccg cctgcaggag ctcccagagg 10560 ctatccacgg ggaggtcatg taccgggcgg ctggcgccgg cgagctcatg ctgccgtacc 10620 agcacaagat cctgatggtg gacccggccg gttgcggtgg ggacgaagtg gccttcgccg 10680 ccggcggggc gctgaattcg tacatccacc tgttcggtgt gggcggtctg cagggcggta 10740 tggtcgagga gaactgcaat gccttgatcg atatggctga agaaatgggc atcaccgaca 10800 tcgtgcttga agcgaacatg ggccacggca ccgcatcact gctgctgatg aacgcccttg 10860 ccaagcgcaa gatcacgaac atcggggtgc gcgacatcta cgccaccacc cagaaagaac 10920 gccgcatcat tgacaccctt ggccctgtgt tccgccgaca caagttcgtc atgcacgagc 10980 gggccataga gatggacgag gaacacctgc aagcgtacag ccgcgagaag cgccgcctgt 11040 acagcctact gttccagctg cagaacatca cgtacgaccg cggctctctg gccaaggacg 11100 accgtgccga cgcagtagct cacctcgtga acgagctcaa aggatacctc gcggtggacg 11160 aggagaagga agcagagaag ttgcaagcca aagcggcccg ggagttcatt gacaacccca 11220 tgggctacac caaagggatg ggccgacgcc ccagtggcac ccgatcccgt ctacgctaaa 11280 ggagaaccaa actatggcca tgtctgttgc caacgctgcc gctgtactga acaaccagaa 11340 cgtacgcgac aaagccaccg ccgtgctgaa cgacttcgag gcgctgagcc aatcgagctc 11400 gcgtctgccc ggcaagcccc tgactggcgc tacatcggtc aagaccgatg tcgacgcact 11460 ttgcaccgcg ctggtcgctg caggagctat cacctcgttc gtatcgtaat aaggaggtgc 11520 catgagcatc acatctatca ctgaggcgga acggatcgag atccgtgacg cgtgcttgac 11580 tgcggcacaa gaaatgcagc aggtagtgca gagtggcgcc atcccacgat cggatattgc 11640 agaggcggta cagccgtctc tgcaggcggc tgcggatgcg ctggggcttg tggttgattc 11700 tilgagaat caggccctgc gcgatgacct tgctaacgag accgacccag ccctcggggc 11760 agcccttatc ggctactcag gacgtaccca agctgacaag aacgctgacg tagttagcgt 11820 gcgggacaag tccgcgaacg caggtaaacc cggcgcaacc gacgacatca cggccatccg 11880 tgatgctgtt aacggtggcg gagcgttcct accagacggc acttatccga tcagcagctt 11940 tgtgaacgtg aatgcccagc agaaggttca cttggagggc gccggtcagg gtaccgtgat 12000 taccaagaat gcggcagcta cacccactgt gctgtcatcc cgtaactcag ataatacgga 12060 ggtgcgtgat ttacggattg atctgacggg tgccaacaac gctactactg gacatgccat 12120 cgccttgatc gacacagacc atgcaaccat tcgagacatg tctatctctg gatttgctgg 12180 cactggcact ggcgttatct cgtactgtgc tgttgagacc gaccataccc agcatgtccg 12240 cattctggat agtcagttcg acggtgacgt ggcccagtcc ccaaacacca acgggttcct 12300 gatcactaat ggcctgtatt ctcgtatgag tggcttatac gccgcaggcg tgggtgctta 12360 cgctattgag tacaagaacg accagaagta ctctcacatc tcggactgcg tggcggtgaa 12420 ctccgtgaac gcgtacggat acggtcagac cactctagat gatacgggtg tgtcctactg 12480 caccgcctcg aacctaatct cgtacaactg cgggaccggg tctgtcattg gcaagggtaa 12540 gtacaacatc acctcgaacc tgttggtatc tgtagacggc ggctccaccc agaccttgaa 12600 ggctggtgtg cgtgcggatg gtacggccaa tgcgaactct tgggtgaact gcctattcgt 12660 aggcaacatc actgagcctg tacgcttcgg cgccaatacc aactttgtat cgggtacttt 12720 ccataacacc cccaccaaca tggtccagct cacatccgga gccacacgaa acgtagtaga 12780 ggtgcagcac ccggggccta cccgaaacag catctttgcg tccagcgtca tctcggatac 12840 gtccgggtcc ggccagactg gcgccaacgc caacgtcgtg ttctgcccag catcgggcga 12900 gtaccgcggt actttctccg gacggtggcg gttccagcat gccccgtccg gattggcccc 12960 ggctagctcc aaccagaaga tggtgctaga gagtaccggc gacacgttct acagcgtact 13020 gaccgatggc actggtttgg taggcctgtc ggcgcagtcc ggctcgggta ccgcgcagct 13080 cgcccacagc gccacctcag actactggca ggcctctggc cataccggcg gctggttcgt 13140 gcgcatggca aacaacctgc tgcgcttcgg tacagacgct cttgccagca tcggcaccgt 13200 caccaaccgg atgggggacg tgttctctct gcggttccgg cccggcgccg gcgcagtcat 13260 ctggacatcc ggcacgggta ctccggaagg cgccgttacc gcacctgtag ggtcgctgta 13320 cactcgcacg gatggtggtg ttggcaccac cttgtacgtc aaagaaaccg gtacaggtaa 13380 caccggctgg gtcgctaaat aaggagacaa catgaaacgt tctgtaatcg ccagcattgc 13440 actggcactc gccctaggcg gatgcagcac ccttgatgca gtgaaggcgg ttacgggcgt 13500 tgtctctcct gacaagcccg agatcactgc gcaggtgggc gcagaaaaca ccaagcaagg 13560 catcggggtg acctccaagg tggagagcac ccagaaggcc ttggaaggcg acgtgaaggg 13620 taatgctacc gtcacccaag ccaccaccac taattcaacg cagaacgcgg cgcgcgacgt 13680 tacagcgcgc accgtgactg tcaccaacac gaactccctg caggtcctcg ggatcgtggg 13740 gggtgcctg agcgctgtgg tgctcatggc catgtacatc gtgcggcggg gccgcaagga 13800 tgaggaggaa tgaatatggg agataagctc accacccccg ctgcgtacac cgcgagtgca 13860 gtcaccggcc tgttcggcat ggtggactgg aacaccgtgg ccactgtcgg aggtctgatc 13920 ctagctatcc tcaccttcgc ggtgaactgg tactacaagc gcaagaacag caaagcgttc 13980 gaggacgcca ttcgcaaggg aggccccgtg catgagccta aagactaaga tcgtcacggc 14040 cctagcagcg agcacgctgc tggggtccgc cctgcttacc agcctgacca gccttgaggg 14100 caccgagtac accgcgtact tcgacagcgc cggcgtcctc accatctgca gcggcgacac 14160 cacggacgtc aggaagggcc agagagcctc tcctagcgag tgcaagcaaa ggctgaagga 14220 tggtattgcc cagcatgcga acgcgctccg tgggctgcct gagagcctcc cagacacggt 14280 tgtactaggt gctgtgagca acgtgttcaa catcggggag tacggcttta actcgtcctc 14340 ccagaagaag tgcttggccc ggggcgacta cgcctgtgct aaggcagcaa ccctgcgctg 14400 gcggtacatc accgtgaacg gcaggaagtt cgactgcagc acccggggta accgcgtgtg 14460 ctggggcctg tgggagcgcc gccagtggga agctcgtgcc atggggaatg agttcaagac 14520 cgtgcaggag gcactggctg ccctgccgaa gtaggagcaa gcatgtcaga gctgacattc 14580 gataacaagt tcgaaagctt caggacattc cgcggcgcgc agatgcgtac cgacatgacc 14640 atggggctgg tgaacaaacg gaagttccgg tgctacaggg agttcaccgg cgctactgtg 14700 ctgcgattta ccgctgcccg ggacttcctc cttaccgcgc aggacctgca gctggacgct 14760 gggcaagcca aagtccagat catgaccggc ggcacgcccg gcggcgtgtt cactgacatc 14820 ccaacgaagt tctgcatgaa cacgatcggt ggtgacgttg ctgggttcag cgctgtggca 14880 gccggcggta ccttcactgg tggcactgag cgcgaagtgc tccgagcgaa cgcaggtgct 14940 ggccaaggtg ctgggcgtcc gtcgcccgct ggtgcccgcc tgctcggcgc tggtacgttc 15000 tatatggtga tcactgtgac tgggactacc agcggtgtgt acagctttga gttcgaggag 15060 ctggataccg ttgctggcgt ggctgtctag gcccgcgtgg ctatccccgg gtgcgcgttc 15120 tacctgcacc cggattttat tatgctcagg cgagggccct ccctcccatc caacagcccg 15180 catgcccccg tacgggtgcg cgtgccagcg tgcgtgcgag tgcgtgccag tgcgtgcgca 15240 caccagtgcg ggagcgtgcc cgcctgtgcc cggaggtgcg cgcgtgccca gctgtgcgtg 15300 cgcgggtgcg cggctgcgcg cgtgtgtcgg cgggcgtgcg tgtgcgcgct tgccttcgcg 15360 cgttcttgcc tcgcgtgcgt gtgctggcgt gtgcgtcact gctcgcgtct gcctgcgtgc 15420 ctgtgcgttg cttgcctcgc gcgtccgtcc ggttctgact ctgctctacg gcgggccttt 15480 cctcgggatt acaccctcga cggtatcgtc tctcggccac ctattcggcg gtctcgttcc 15540 tctacctagt gcagagctat tgcggaggct ctatctcaag ggtttcagag gtgcctctgt 15600 taggctgtag aactaccgtt cgtcgggtct gggtttactt cctgtgctgg atgggccatt 15660 ctgtagccac tcccaacgag gtaagaccgg aaggcacttt agggagtaga gcctacgggc 15720 tgcgctggtt cgccagatgc gggacttcca aggcttgaca gatgcaccgc aacaccgtag 15780 tatgcgagac gcaacactgg acatggataa caagcccttt gggcggcgac catgcacggc 15840 gcagagcgag ccggggtata acaagcgcgg ctgtctggag cgatgcgtaa tggccagaac 15900 gggttgggtc cgagccaaca tctagcggcg agcccttggg atgcacggct aggcttgaca 15960 ggatgcacgg gagtcgatac actggctccc aacaaggcaa acgggccttg ggtggcgtgg 16020 atagcttact gggtgcaagc tcggtcggtg aaccgcaaag ctcgaactgt actggaatga 16080 ccagtgtgga tgctagacgg cgaagctgcg caagtggtgg ctgcctgaag gttgaagcaa 16140 caaatgccgc gatggtttgt agaagtggcc ggttgcccaa gagtcccgac tgtacataaa 16200 acggactcga tgcgtttgcc tgagcgttcc tgtggtataa caggagaaat cagacccagc 16260 ggggagagcg cggctgggag tatgctgagc tacagggtgc ctacgtcgaa agactacgcc 16320 aattgccaag gtctggcgcc tgtgtagcat cctctcaccc tgttaccggg tgactgatga 16380 tggctgacgg acagccgaaa ggagactagc atggctttcg aatcgaaagg caacgttcgg 16440 tacaacaagc tcgccaagcg cgaagcgaag gtacaggaac tgcgcaacga aggcacaccg 16500 gaatcccgta agcaactgaa ggctatgctg gactggcgtc ccgagtggga gaacgctggt 16560 catcgtcgtg cggtcactcc gaacggcggc atgatgcaca atccaaaggg taagcgtcac 16620 gtcaagggca gctctgccaa gtgtgaccgt atcaaccgtg gccaccgggc caaactcaat 16680 caaggcaaag gtatctaatc atgaaactct tcgaaggcgc agccgcaatc aacaaagaaa 16740 tcggcctgat caagaccgcc ggcaagaagc tcgacgaccg tattcaggtc gccggtgtga 16800 gcgtgatcgc ccactgcggc cagcacaacg atgtcactgt ggtgaacaac ctgttcctcg 16860 ctatgccagc gggtgctcgc aagaaagcgc tggccgagtg gctgttgaag ttcgggaacg 16920 tggccgcgaa caccgacaag gcgtccagca agactgcgcc cttcgtgtac gccaaggaca 16980 agcaggcgaa cgtcgaaggc gccattgccg agccgtggta cgacttcgca ccagagccgg 17040 ctgtggtcac catgttcgac ttccaagcaa tgctggcgag cctgctcaag aaagccgaga 17100 aagccgagca acagggcctg aagatcgaag gcgccgaggc gctggcggcg gtgcgcaagc 17160 tcgccaaggt gaagtaagac catggccggg ttcgtgctcg gcctcgtggt gggcttaggc 17220 atcggctgtg ctggtgcctt ctgcctgctg gcgtggttaa tcatcgggag acgctgatat 17280 gcctattctc ggcttactgt tcgctttgca gggcatgtac cgggccttta ctccaccggt 17340 gcgcaaacaa cctgaggtac gacatcatgt ggcaagcact gtgctacacc ggtaatgggt 17400 acgatgtgtg ctggagacca ggcaacatcg ccacgaacat ggtccggcag agtgacatat 17460 gctggccttc tgccccttac actcctgtaa ggctctgccg agccgtgtga ggtgcgtcat 17520 gctcaggata ataggttggc ttgtgttctg cggtggcgtc gttctggcgg cctctggtgc 17580 gctcacaggg tgtgcctcag ccgttgcctc gggcgggatc actcagcaga acggtacttc 17640 acattggcag gagtacaatc aggccgtgca agacgagtgc gacaagcagg tggtgaagat 17700 cgccaccgag gccggcgtca ccgccgacaa catcaaccgc ccggatattc agcgggcatt 17760 gcgtgaactg cgctggcaat gcgcccatca acaagggctg acatcatgag tataccggtg 17820 cgtactacct tcattcaaag gtatgctgct gaaatcaagg ccacgtacaa attggatgtg 17880 cagttacagg tacgtcgtat ttcaagcggg cagattatgt ccccaaatct gagccgggca 17940 ttgactctta cccttgcagg tgggatacag ggatcagcgt cttataacag ggtagtatgg 18000 ctgctaggta cgggtgtact tccagataag ccagttgtaa agactggact gggttatgca 18060 tgtaacggtg taatacttat gcccgagtgt agcggagaac ctgcggcaca aagggcagca 18120 ggtggggcct tgcgcttgga tgaggccagt ctactaaagc agtttgatgc cgtagtatcg 18180 gcccttaatg aacttggcat cgacccagtt cggctagtgc gccgagttac cacagaggtg 18240 cccttatgat cccccacgca ttcagcattg cagatatgcc ggcaatgttg cggctgctag 18300 cagatgagct agaggaatct gccgacgcat tggcggtagt agtgtgtatt aataggatgg 18360 gcggtctaga cctgcgcggt tacggtcggg ctgactggcc agacgcagta tgtcttctta 18420 agcaagctat aggtggccta tgatcaagct cccaaagccg cgcgcccgtt ttatcgacgg 18480 gcattggcat gtgtactcca ctggtacgat cacctgctcg ggctggacca ttggccaagc 18540 cctactggcg tgggtgcatc ggaactggac ccagagcagg atcatgcgcc catgaaaccg 18600 atcatccagt ggatgccggc ggcgcataac atctacatca ctaagagcgg cggtatgcaa 18660 tcaggttatt gggcttgcag gcaccgcaga ggtttagcct ataaatcggg cgtgggccgt 18720 acaccgcgtg aggcgtacga gatgtgggca cagatcaacg aggtgcggcc atgattgcaa 18780 cagcaaatgg tgggcgcgct gccgtaggca ccgagctcga tgagtcggtg cccgctgctg 18840 agcgcgacgc aatcatcgaa cgtgagcaac gccttgcagc gtggcgggca agcctgccga 18900 aacgtaagcc ccgggcggat gagctgcccg ggccgtgtga aacctacaac tgtgccggcc 18960 taggctggac ttgggagggt aagtgagcga ctgcattcca accaaacgcg ccaagaattc 19020 acagggctat ggctatgtgt ctcgcaatgg tcgaatggtg ggcgagcatc gcgttgttta 19080 ctgtgactac aacggcgtga cgttagattc aatcgctggc ctagtagtac ggcacgcatg 19140 cgataacaga tggtgcatca accccaaaca cctgatgctt ggcagccagt ccgacaacat 19200 gaaagacatg gcagagcgcg ggcgtacctc tgttcgcaga ggcgaggata acccaaatac 19260 aaccctaacg tgggatgacg ttagacggat tcgaactttg cgtgacgacg gcgctaagca 19320 agtcgacctc gcccgcaggt tcggtgtatc acaaggaacc ataagtgcaa tctgctcgca 19380 caagctatgg cgcgagccct aaacctgcag cccattaaag gacacgacaa atgagcctga 19440 atttcgcaat catgccagcg gatgccggcg cctcccaaga ggccaccgaa acccgcgaca 19500 agctggccaa gattatcgag agcgttggcg ttctgagtga cgcggcccct gagccgctga 19560 gcaaggaccg catccgcgac ctgcgcaagt ccggtgccga gttatttggt atgttctacg 19620 acggcgagta cgcccagacc aactacagcc cgaccataac cactcaggcc cggctcatca 19680 atgggctgac cgcaggcatg ttcaagatgg ctgcccgcga atcacgcagc accagctcgc 19740 gtgacctgac ttacatgtgg gaaggtatcg ttaaggcctt caatgcggct gtgcatgcgt 19800 cgaagctgaa gatggacgac gagttcaagg ccaccttggg cttggcgttc ttcaaggact 19860 accacggtgg gcacgtgcat gtgtggctgg agcgtaaagg cctgttgcca cggccagtgc 19920 agtattggcc gttccaagag aacatcgagg agttcaggct gaacctgttc gtgcactacc 19980 gcctcggcaa tgacggcgca gcggacaagg ccacccgaga gaccttgaat caggtactga 20040 aggaccagtt cgctgtggat tggaccggta attcgtggcg cgtagagctg ctccattgcc 20100 gccccgtcat gatggtgtac ttcgacaccg atggtccatg ggctgctgga ttcgctagcg 20160 ccgatgacgc ttgccgcggc cgtttcaccc gctgcccgct gggctcgtgg ttgagtgcgc 20220 gtggtgctga tgatgagacc gtcaagcagg aagtggagcg catcaaggcg atgaattcca 20280 ctggcgactt cgaactgtac ccgaatgact gcttgttcgg tgatttgtac gaggcaggtg 20340 cgagcgacgg cgtgagcagc tgcatggcct gctcccgtga tgagttcaac ctgtgggact 20400 accaccaccc aaccgatgcg tacagctctg cgtaccatgg tagcggtgac aacgggctgg 20460 tgctcgtggt cgagcgcaag aacggtaacg tggccggccg tggcatcctg aacgtgaaca 20520 ccaagaagta cgtgcgctgg tacggtgccg tgaccggtga tcgtgcactg acccgggcag 20580 gcatgaggcg tacatcgtcg gccttgaagg gctcgtggct agctatgctg cggcatccaa 20640 ccatggaaag ccgcatagtt gcgccgtacg tggacggtga ttaccaaggc gcccgtgtgt 20700 cccggtctga acggcgtgtg tacttcgtag acgatgccgg cgatgccctg tgcgaaactg 20760 gcgggtacta cgacctcgac tctgtgtact gcgtggacat cggtgatcat aacgaccgct 20820 gcgacagcac gtaccagtgc gagcacaaca actgggtaag cgacggcttg gaggacgact 20880 ggcgctgccc agtcattgct gaatggtgcc acgaagacga acgcgtcgag atcatgctcg 20940 atggcgaaat ggtgtacgtg tgcaaccggg ttggcgagca acgcacctcc cagtacctga 21000 acgtcatcaa gagcccagaa gacgacccgg acgctgtgaa ctacaccggt cgcggccgct 21060 gggctactgt gcaggataac accccgtaca ctccgccgcc acctgtacag cgcttcagct 21120 gggatgaact gccaatcgcg caggccgcag ccccggcgcc tgtacttgca gccgatattg 21180 cgcagcagct gccagccgca atgtccatct atgcacaaat gggctgcacc tgtactgact 21240 gccgcatcga acgtggagag cttctgtgag caattcaatc gaggttcccg aggtactgaa 21300 ggccctgctg accacccgcc ggccatacgg cggtgagggc gaacgcgccg cgaggctgct 21360 cgttcttgac acggtgttcg aagctgggta cgtcccgacc gtagatcagc atgggaacgt 21420 gcaggtactg gtggggaatc gcccggacct cgtgttcacg gcgcaccttg acacagtgca 21480 ccgtgccgat ggcaagcagc tgctgtggct gttcgaggac accgaggaga tcatggcaga 21540 cgggctggac aacaagccca gcgtgctcgg tgctgacgat gccagcgggg tgtacctgct 21600 ccggagatg atcaaggcgg gcaagcccgg gcggtacctg ttcttcgtgg gtgaggaatg 21660 cggcggcgtc ggatcatcgg cgttcgtgca agaccaccca gacttcagcg ccctgatggt 21720 cgtgtcgttc gaccgccggg cacgaaggac atcatcacgc accaagctgg gtactgcaca 21780 gccagtacga cgtttgcgct ggcgctggcc gatgcgctga acaagcagaa gcccggcgtg 21840 ttccagtacc tgccatgcga cggcgggatg tacaccgact cgaaagagtt cgcggagctc 21900 gtgccagaat gcacgaacat cgcggtgggg tacgaccacg agcacacggt gaacgaggtg 21960 caggacctgc ggcatctgct gaacctgcgt gacgctgtaa tcggggtgga ttggacggcc 22020 ttacccatag cccgggtacc cgaacctgac accccatggt ggcccacatg gaacaagaca 22080 ctgggccgtg actggcacaa cgatattccc gacgacccac tgcatcagcc tgtgcgaggc 22140 gcagtggcca tccgcaacct gctcaaagag ttccgagatc acgcttggaa caacggcatc 22200 gacgacttcg acgagctgga caacttcctt caaaagatag aggacatgct atgaccatcc 22260 tgacccgtat cgcatcaggc ctgctgttca tcgaccacca caaggccgcg ctcacctctc 22320 gcctgctcag cggtgccgcc cgactgaaca ccctgcatgc cgaatccttg gccaacaaag 22380 ccggccgcaa gctcgtcacc ggcgaggcta aggcccgccg actggccgag tcccgtcgtg 22440 agcaggcgca tcgtgcgctg gcgtcccaac tgcggagcat ccaggaggac ctgcagaccg 22500 atctcgccaa ggtgtacatc gagcgcgaca aggccgctca gacagcggca tcgtacgagg 22560 ctgtgggtta ccattgccat gcctcggcat gcgtgcgaga cgagcgcgca gcggtcctga 22620 agggcatccg cgaggagctg cgcagcaatg gctaacaaac tcgtcgagtt gctcgaactg 22680 caggttcgcc taccactgtg gctgttcatc gccggggcaa gcagcctcgg ttatgacgcc 22740 atcaacgcac tgggagtatt gggatgaaca acaaggtatc actggccgca tccgtaatgt 22800 gt; ggcggagtgg ctgaccgcag cccaagccct cccgattggt ggcaagcgca ggatcaagca 22920 cggcttcgag gccaccgccg ccatggatgt gtggaacaac gaagatagct ggtcctgcta 22980 ctgccatcgc tgccacgagg gcgggcgagt gccgaaggaa catcagcggg tgcggcgcgt 23040 catggtcgag cccgaccgcg tagcgcctgt acctgcaacg gtaattcgct tggcagatgc 23100 gagccgctgg gaacagcagc ggatttggga attactgtgc cgcaagggtt gcccgccggg 23160 cgtaatcccc gaggaggttt tatggtacga aagatcagtg gatcgtctga tgctgctatc 23220 agacgacgtg gcgctcggga gggcgctgga cggcaggcgg atgccgaagt ggttaccgta 23280 tggggcgtgg cggggcaagc cgatgatgtg ggcgactcgt accagtgccg aggcaacgga 23340 acctgcggac tctgcgccgc ttgggtcgct gatcctgacc gaggacgcgc tctctgcgta 23400 caaagtggcc aaggccatcg acacctatgc gcccagaagc tcgctggacg tatgcgcgac 23460 cctcgggact agcatcaccg accggttcct accgtacaca ctgggccggc ctgctgtgct 23520 gtgtatgtac gcggcgatg ccgccgggct gcgtgggtac agggccatgc gtcaacgtct 23580 ggctgtgtgg cagcagcccg tgttggacct acgcccagag cagggcgacc cgaagaacaa 23640 cgacctagca tctatagcgg aggcactgag taagtggatt tgaatatact cgccgcccta 23700 cgagagcgga ataagtttcg gcagttacgc ggagctgtgc ccgactctct gctgggccaa 23760 gaaaccgtga gcatgctagc gtggtacgct gcgtacttca acgcattccc cgacaaggac 23820 tacgtggata ccgacgtact gcgctcgctg ttcaccttga aggtgggcag caccgccacg 23880 ccagagcagc tggccgtgat gcgagcgctc atgaacaaac tcgacgagcc agtcgatccc 23940 gaggtaatcg acggcatcac cagtcagctg tacgagcgcg acttcgccgg ccgtgccgcc 24000 gccctgctga accagtacga gaacggtggc gaggtggaca tcacgtacga gctgaatcgc 24060 atggccagcg agaacatgcg ccggctcagt gcggcagccc cgagttcgta cattgacacc 24120 ccgatcgagg atatcctcgc agacttcggg caggaccgcg ggctgaagtt gcctaccaca 24180 gccctgaagg agaccattgc aggccttcag ggcggggaca gtgtggctat tgcggggcgc 24240 ccggacaagg gcaagacgag cttcctagcg gcctctctga ctggtttcgc taagcagctg 24300 aaggggctcg gtctggacgg acgcccgctg ttgtggctga acaacgaggg ctcgggcaag 24360 cggatcatcc cgcgaatcta ccaagcggcg ctgaacatca cgttcgagca gatgctgcag 24420 atgtcgaacg ccggcacgct gcgcgatgcg taccagaagg cggtggatgg gcaggagatt 24480 cgcgtgaagg attgccacgg cagcacgctc ggacagcttg agcagatcat cgaagagatg 24540 aacccgtgcg ctgtggtatt cgacatgctc gggaacttcc gcatgcctag cacgggcggc 24600 gggaacaaga ccgacgcact ggagcagatg tggcaggagg tgcgggagat ggctgtgcgc 24660 cacgacttcg tggccatgcc aaccatccag atcagtgccg acggcgacaa catgctgttc 24720 ccgccgtaca gcgcactcaa ggatagcaag accggcgtgc agggcgcgtg cgatgtgatc 24780 ctgatgatgg gcgcattcaa cagtgtggac ttggacaacg tgcggggatt cagcacgccc 24840 aagaacaagc gccagatgcc gggcaaacca agcaacgtac agagtcaggt gttcttcgat 24900 gcaccgactt gccaattcct agatggagaa tagcatgacc acgaccgctc agaagaaacc 24960 cgcagccaag aagccgacca ccaaggccac tgcagcggct gcaaagccag tgatcgatga 25020 gaacgctctg ctggcccagt tcgctgcagc attgctgcgt gacggcacgg gccgcattga 25080 tgccagcgat gtggccctgc gccagcgagt ggtggacgcg ttcaaccgcg cccgcgtcat 25140 ggtggacgag ttcaaaattc aaatcggagc ctgatcatgg cacaagtcct gctggatgtt 25200 catcccggcg cattcatcag cgccctacgc aacaacatcg accgccgcga acaaatccgc 25260 agcgccaccc acgacaccgt gaccctgtgc aagaacattg acagggcgct gtccgtggcg 25320 gcagtggtgg ctgccgaggg caacgacaag cgtggtgggt tcctcgttct ggatggagag 25380 ttgcaaggcc tgcacagtat cgacaagggc aatggcgact ggttgatgcg cgccgccgtg 25440 gggcagtacg gtgcgaaccg tctggacacc ttcgacatcc cgaacctcgt ggccttgtac 25500 acccgccacg gattccgtga ggtgctccgt gagccgaact tgaacggcgt aggcccagat 25560 gttatctgga tgaggagaga ctgatgggcg ccaagagaga gcgcgtgctg ccagtggact 25620 gccaccgacc ggtgaactgg aacgaggcag cagtgcagcg cgtcattgat aacgtcgggt 25680 atgtgtacgc ccagaccaag aaggatggca tccgattcca tgccatgatc gatgctaacg 25740 atcgggtgcg tgtgctgacc cgtgagggca tcgagatact gtcccttgac ctgcacaagg 25800 aacgcctccg tgggctcctg caagccctgc ccaagcagtt tgcgctggat ggtgaggcgg 25860 tagtaccggg tatcccgttc gaagaagcca gcggcatcct acggcgccac aaggccatcc 25920 cgctggagga ctccgtagag ttctgggtat gggacgcgct gcccgttacg gaactgttgg 25980 ggtacgaggc cgggactgca acactggacg agcgtatcga cacgctggtg caggcattcg 26040 gcagcacgcc ggagccgcag gcgcagatcg tgcacatgga accggtgagc agcatggagg 26100 aactgtacct gctgttcgat gcccaacgtg agctgggcga ggagggtctg gtggtcaagg 26160 acccgagcct tgcaccgcgc aatggcaagg tcagcggcgc atggaagctc aagccgagcg 26220 acacctgtga cggccgcatc accggtgtgc tctggggtac gcccgggctg ggcaatgccg 26280 gcaagatcat cggcttcacc gttgaactgg agaacgggaa cctatgcgat gtggatggcc 26340 ttacccaagc agccatggct gaatacaccg ttcaagttga gaactgggcg cactgtcaat 26400 ccccaccaga atacgtatcg ccgcacccat tcgttgggcg gtacgtagag gtaagctaca 26460 tggagatgac cgccaagggc agcctgcgac acccgaagtt caagaccttc cgggatctgg 26520 attattgccc cggatggaaa tgttaaatgc atgcatagac catgggcgcg gtggtgataa 26580 gcaaggttac gcgcaaactc acggcacccg tttgcatcga aaggtgtatg ccgccaagat 26640 gggactgact cttgagcaaa tcaagggcgt cacagtacgg cacacttgtg acaatacgcg 26700 gtgcattaac ccagaccatt taattggcgg caccatgtta gataatgcca gagaccgtgt 26760 tgagcgagga cgcaacgcca agacacagcc atcccgttgg aagcttacag aagagcagtg 26820 ccgagctatc ctacgcagat tcagcccacg gcgtgttggg ctgactgcac ctgatggtat 26880 aacccaaatc gcccgcgact acaacgtcga tcgtaatgtc attcgacaag tgctgaacct 26940 tacctacccg gggtggaaat catgaggcgt attgcaggct tcttctggta tcgcggcgcg 27000 attgtacgca tccaatcata caatccgatt ccgccgtatg agtttacgga cagtacggcg 27060 aaccaacgac gcgcccagtt ccattgtttt gggctgttgt tcaatcctaa agccttgtgg 27120 gtcggcgccc actacggcga gcatcacaag cgttggtgca tcaacattct gccatgcctg 27180 acagtgtggt ggaccaagcc aggaggctac ctcccgtaat agaatgacac tctgtgccca 27240 gaatgggaga ggattcgatg tacaagatat tcatcactga cttagagact gagaacaagc 27300 cttggtacgg tcagatagca tcaccattca accctgagaa ctacattgta gcccctgggt 27360 ggcgagtgga caccgtgcat gacgatggct ctgtcgaggt aggtgagacg aagtggaggt 27420 acttcgcatc cgctgaggag gcagacgccg gggccgactg gttcgacgag ctggaggggt 27480 gcgccatcat ggtggcacac aacagcatgt tcgagcagaa gtggttcctg agcaagtacc 27540 gggagcgctt cgaggcgttc ctgaaggccg gcggtaggct agcatgtacc caaatggctg 27600 agtacctaat cacggctcag caggaactgt acccatcctt ggacgagact gccgtgaagt 27660 acggcggtac gcacaaggta gacggggtaa agattctgtg ggagcagggc tacctcacca 27720 gccagatcga caaggacttg ctgatcgaat acctcgccgg cccgtccggc gatatcgaca 27780 acactgcgct cacgttctac ggccagcagg agaagctggc agagatgggc atgaccgaca 27840 tgtactggga gcgctgcgac tctaagctgg cattcggtta ctgtgagtgg ttcggcctgc 27900 acgttgatcc agaggtagct gagcgcaacc gcgcagcaca ggaggcggag atcgcagaga 27960 tgcgggcgaa gctgaacgag ctgctgccca aggacatgcc cgactggatc gagttcagtt 28020 ggacgagccg gtacgatgtg tcggccctga tctatggggg cccggtgaag ggtcgcttca 28080 aggggccgta cgacccgccc cagtacgtca aggccgacta cgtgaagact acggacggtg 28140 caaccttcct gttggaggag atggatacgg tcacgctgga cgccggcgcg gactgggagc 28200 ggtacaagtc gggcaagaac aagggtcagg tcaaggtgtt ccgcgaggac accgacgagg 28260 agaagctgaa gtggcacgag ggttcgtacc ttatgcccgg gctcgtagac ctgaagtccc 28320 tgcccgatgt gatcagcagc aagtaccttg gcaagcgcgc cgagttccgc ggtgcccaga 28380 ccctgtgcga tggcgtgacc ccggtataca gcaccgctga ggaagcgctg aagggcttga 28440 agaactacgt gcctgaggtc aagatcatgg ttgatctggc caaactggag aaggataccg 28500 gtacgtacta tctgcgcacg gagtacgatg ctgatgggaa tgtcaaggaa gtcaagggca 28560 tgctccagta cgttcagaag gaggacaaca tcgtccacca cagcctgaac accgaggcga 28620 ccgtgaccac ccggctcagc agctcccgcc cgaacctgca gaacctgcca cgagcagacg 28680 aggacgcaga cggggaggcg aagtcccgcg tgaaggaaat gttcaccagc cggttcggcc 28740 cgcagggcag gatcatcgag gtggactaca gtgcgctgga agtcgtgatg ctgtgcgcca 28800 tgaccaagga catggacctg ctgaagctcc tgcaggctgg taccgacatg cactgctacc 28860 gtttggcgtt ccagctgggc gagccatacg aggaggtgct ggccaaggtc aaggatgaga 28920 gtcaccctga gcacgggaag tacaaggccc tgcgctcgaa catcaagccg ctgagctttg 28980 ctgaccagta cggcgcatca gcagcggggc tggcgttcaa cactggctgc tctctggagt 29040 gt; aggtgatcat cgacgaggtg aaccgcaccg gcagcctgcc gggcagcatc caccgcgaga 29160 tgtacgacaa cggccagtgg ggcgtgtacc gtcgcgggta cttcaccggg ccgagcacca 29220 ctcggtacag cttccgccag cagcgcactt gggacaagga gctgcggcag gaggtgatgg 29280 cgtacaagcc aacccagatg gcgaactacc cgttccaagg cgaggccggg ttcatgatgt 29340 cgtccagcat gggccgcatc tgccgctggc tgatctccgt ggactggttt gacaatcagg 29400 tatgcttgat caacaacgtg cacgacgcag cgtatctcga cgcagcaaac gaggaggtag 29460 gccgggtggc agcgcttggc accaagagaa tcatggagga cgcaccacgc tatctcaccg 29520 agatgtggcc ggcgttcgac atggccgatg tacccttccc ggcggctgct gagatgggcg 29580 cgaacatgat ggagaaatac cacgtcgagt gaggcgtggt ctgtacttgc agcgtaatat 29640 cccaatcccg aaacgaggaa acatgaatgt ctcttgatat cctgaacagc ctgatcgacg 29700 aagccatcga actccagtca gttgacatga ccgaggaagg cactggcggc ggtggcctga 29760 tgccagaagg ctatgccatg gcgcggatgt gcacctacat cgagctgggc cagcagccgc 29820 aggaattcca aggcaaggcc aaggcgccgg ccgacgagtt caaggtcggc ttcaagctgt 29880 acggcggcga ggacgactgc tacgaaggcc gcttcatcag caccttcgat ctggccctga 29940 gcaacaacgt gaagtccggc gccaagaagc tgttcgacaa gctgaactgg aagggtgacc 30000 tgcgccacgt ggcccaagcc ctcggccgtc cgttcttggt accaatcact gtggtgacca 30060 acgcctctac caagaagcag tcgaaccgca tcgacttggg tggcatcctg ccgccgatcg 30120 acccggtgag caagaagaac tacccgattc ctgatctgga cccggccgac ctgaagtatt 30180 tcttcttcaa caagccgacc aaggagactt gggactccct gttcgtagat ggcacttggg 30240 acgacggcaa gtccaagaac aaggtgcagg agaagatcat gtcggccctg aacttcccgg 30300 gctccgatct ggagcagctg atcagcggcg ttgtgctgcc tgacttggcc ccggatgagc 30360 cggccgacga gccagagccg gagccagtca agactgcgcc caaggccgct gctccgaaga 30420 agcctgtagt gccgaagatg ccagccatgc cgaaactgcc tgtacccgac gcggagtaag 30480 ctatgagcga cctgaccagc ctgctggaag gtctgccatc ccagttcgag cgttccgtct 30540 ccggacggac gctcattctg gatgctgact tccccgcgta tgaggcctcg gccacggtga 30600 agaagttgcc caccgccctg acccgcttcc agacgctggt ggaaaccgag gtgttcctga 30660 ccagctccat ggatgtgcag gtgcacctga ccccgcgtgg ctgcacgaag tgccggcgat 30720 acgactaccc cacggccaag ccttaccaag gcaaccgtga aggcaaggct aagccgccgc 30780 tgctggagcc gctgcgcatg gccatcccga gccacccttg gcctgagcac tggtctacgt 30840 tcgcatggcg tgaccgcgag gccgacgacg gcatgatgat gcacagcttc gccctcgggg 30900 caaacggact ggtctgctcc ggcgacaagg acctgtgcct gaccccacat ccgtactgga 30960 tcatcgacga gggccgggtg gacatcatca aagaccggtt cggctggatc aagcgcaagg 31020 agtgggacaa ggactggaag atcgtcggcc acggcacgaa gttcttctgg tgccagatgc 31080 tgatgggtga cccagctgac aacgtgcagg gtatcctgcg ctggtacgga aagaagtgcg 31140 ggaagcaggg tgcatgggag atcatgcacg agatcgacaa cgaggatgag gcggccaacc 31200 ttgtgctgca ggcgtatgcc gacgcagggc aggacttctt ggccgaggcc cagtgcttgt 31260 ggctgcgccg gtcgctggac gactgcgcat tcaagtacct gagtgagctg gaccttaagc 31320 cccaactgcg cgagtggatg ggcatgctgc acgactacca cggccaatac ttggccgcta 31380 ttgacgaaca gaaggaattc gaacgtggcg aagatcaatc aagcgaaggc tcgggaactg 31440 aagcatcagg gaaccccagc ggcatccgtc tcccgtggga agactagtct gatcggtgac 31500 ctccgggccg ccgcgctgga cgcgtacttc gccggcaacc accgcgagct ggagaacatc 31560 atggcccgcc tgaagaagga gagcccatac cacagccgcg aagtgtacga agaaatcaac 31620 gagctgatca gctacggaga gtgacatggg caccgtaacc ctactgccac cgagcggccc 31680 gccacctgtg aatgaacagg tggtagaaat gttcgaagac ttgttggccc gcgctaaagc 31740 gggagagatt gatgcagccg cagtggcgtt tatcagcggg cgtgtgaaca tcctgacgac 31800 gtttactgat aacgatgagg atgtattctc tgcgcttatc ggcgcggcca ccgtcctgac 31860 tacccgcatg gccatgggag actagcatga ccgttattca gtacttggca gcgttcgctg 31920 acctactcat tacccgcgcc agtaaagaag gcattgcgcc ctgcactgtg gtgtaccata 31980 ccaacgatga cggcggtggc ggttgcgcca tcaccggtcc tgccggcggc tgcatcacct 32040 gcgatacaaa gtacccgctg gatggcgtgc tcaaggacga ctacgtgccc gagctgacta 32100 cagaaatttg gcagctattc ctacgacaag agaagccgca ccgcttccca ccagtaccgg 32160 agacgctgca atgagcggtt acagcgggca cactatgagc aacgggcgac gggtgcggac 32220 tgacccgcgg ccgcatcgta agcaggcatc gcgcctacgt caggcgcaac gaatggcgga 32280 acaggaggat gccctacgtg accccacgaa agctgactag atcccaactg cggcccctgg 32340 gcatgcggat gctgcaagag caaggtggcg tgtgcgccct gtgtggcaag ccgatcgacc 32400 tgagcatcaa gggtgagatg gtcattgacc acgaccacga gacaggcctt gtccgcggtg 32460 ccctgcaccg ctcctgcaac gcagccgagg gcaaggtggc caacgccgct ggacgctggg 32520 gtgccaagag catgtcgtac acggcgatcc tgccgtggct gacgaacctg ctggcgtacc 32580 tgcagaagac cccacagcct tacatctacc ccacacacaa gacggacgag gagaagcgcc 32640 ttgaggctaa cgccaaggcc cgccgcgccc gcgccaccag acaggcacgc caagtcgtgc 32700 gcaaatccgt aatcaagaag gactgacaat gcgcctcctg aaacttctga cgaaagacca 32760 gcatatcgaa gtgctgaccc gccatgcctg caacgtagag gcagcgcagg tatacagcat 32820 cgccatgggc cagccggtga cccgccagaa cgtggcgtac tggcgccgca tcttcatcga 32880 cgagggcacc aaggccaagg ctgaccgact gctggccgcc ggccgagcac tgcgcaaagc 32940 tgagccgggc gacgacgtag gcgacatctc gttcgtgccg aaggtggccc accgcatcct 33000 cgtgatcgga gacctgcatg agccgtacgt ccatcctgat gcctacgact tcctcgaaca 33060 cctgaaggcc gagtacaatc cggacctcgc tgtgcagatc ggggacgaga cagacggcca 33120 tgccctgagc ttccatgagt cagacccgaa cctcgacagt gccggcgtgg agctggagaa 33180 gt; gaaccacggc tcgctggtgt accgccgcgc gaagtacagc ggcatcccaa tccagatgat 33300 caagtcgtac cgcgagatcc tgttccccgg gcacagcgcc tgtggctggc actgggcctt 33360 caactggaag atcgagaccc cgctgggtcc agtcctgttc cgtcaccaga ccaccggtga 33420 tgttatggac gccgccgccg ctgagggctg caacctcgtt gtgggccacg agcacggtaa 33480 gttcggctcc cgctggggtg ccaccagcat gcgcctgttc tatggtgcat acaccggctg 33540 cctgatcgac aaggacagca tggccttcgc gtacggcaag acgtacccgc gcaagccaat 33600 cctcggtgcc ctcctgatta tcgagggcgt tcctgttaac gtgccgatgc tgctcgacag 33660 cgacggccgc tgggtagggc cacagcaatg agtcatgatg ttaatgcgaa gtgtgtgggt 33720 tgcggaggct tcttctactc gcaagagccc tttggggatg tgcggtgcga gtgtggcacc 33780 gtgaactttg ccaacaaggc catccaacgt tatcgagccg ccaccaagga ggcacctgtg 33840 actgtacctg ctcaagcaac gcccggcaac gccggcggta tgaagttcga cgcgggcaag 33900 ccccgctggt ccctgctgat gggaggtatg cccggcgccc tgactggggt ggctgaggtg 33960 ctcacctttg gggccaagaa atacgcggcc cacagctgga agacggtgcc cgacaaccgc 34020 gagcgttacc gcgatgcgct gtatcgccac ctgaatgcgc tggaatcggg tgagctggta 34080 gaccccgaaa gcggcctatc acactgggac cacatctgct gcaatgcgct gttcctgtct 34140 caactggcta aggacgcaga caagtgatcg ccgccctagc ggtgttcacc gcagcgtacg 34200 ccaccgtaat gctgctgggg ctgcagtcca agctcatgcg cgacaacaac tggcgcctga 34260 gcttcttcac cagctggttc atcacgttcg cccaaacggg cagcacgtac gccatcgccc 34320 acaacgccct gcccatcgag tggttcctgt tcttcagcgg cctcggcggc agcaccggca 34380 ttgtgtcggc acacttcgtt tacgattggt acgacaagag gtttaaacga taatggaaga 34440 cacctacgca tttgttgaag atatgctggc cgccttggag tcggattgca atttcctaca 34500 gggattatgg ttggatcaac gtattccaca ggatatacgc gatgctatta tggcacgcat 34560 tgaaacccaa gaccgccgca atgaagactt cacggcgatg ctggagagcg cataatgctt 34620 ccacaactcc aagcagcagt acacaaaggc ctaagcgaca gcctgcacgc agcgatcgac 34680 acaggaaacc aagaagaagt atatggggcg gttgataccg ccgaagaagc ctttgcactt 34740 ggccagattg atgccatcca gctgggtgac ctgctggatg acttccgcat ctgggaggag 34800 agtttgctgt gatgttcgac cttgataacg tggactacgt aggccccatc tgcgccctga 34860 ttgatgatgg gaatttcgat gccgcccgcg ccctgttgca agaagtacct gccaagctcc 34920 gcgagtccgt acgctggact atcgcatcca tgaagaacat cgtcctctga ggtaggcatg 34980 gacctgattc aacggcaaat caacatcgag caagagtaca ccgcccaagg cattgcgctg 35040 gcaatcaaga agtgggagac ggacattgcc gagggccgta tcgcggacac tggtgtgggt 35100 aagcgcatgg tgatacgtgc gctagccggt gttcgctccg aactggatag catctgtaat 35160 gccggtacac gcggcgtggg cggccgctat cgcagcctgc tacgtgatat cgggctggac 35220 attgcagcgg tcatcggcct gcgcgccggc ctcggcttag tcaccggcca gcgtgcccgt 35280 aacggcgagc cccttgtgca ggacctcctt agcgaagtag gcgcacacct agagatcgag 35340 catatggcgg tcaagctgaa ggcagctgcc ccagcatacc taggaagggt cttggacagc 35400 ctgcaggagg cccgtacgcg cagttctaac cacagacgac gtaccctaca ggctaccgcc 35460 gataacgtct ctgtgggctc tgagaacgtc acatggagca ccggggagcg agagggtgcc 35520 gctaagctgg tgctggaggc gctggttaat ggcggggtgc tggacatgta ccacatcccc 35580 aaaagccgtg gccagagctg ggtggccctc aaagccagcg atgccattgc tgcgcacgta 35640 gatcgcatgg gcggtgcggt gcgggcattc atgctgtttc cgccaatgct tgtgccgccg 35700 aagccgcata cccgcgacac actgttctct ggggcgtcat acctcacaga gggtatgtca 35760 atcaactcgg caagtattcg agtacgctcc cggcgtgcag accatcgaca gtggatcaag 35820 gacaatgtaa gtgatacggt cctaactgcc gcgaacgtgg ccgcatctca gccgtactgc 35880 atcgatgtgg agacggcctc gatcctgcgc gacctgttct cccaaggtgt ttacaacggc 35940 atcgcgggca taccgtcacg tgatcccatc cagccgccgg cgtatccgct caaggagaac 36000 tgggaccgcg aggaccctgc tctacaggag gagcacgctg cgtggaaggt gctggctcgg 36060 caggcctacg ccgacgagct ggcccggaag tcgcacgttg tgtcgttctt gcagaccctg 36120 aagtatttga aggagtacgc aggagatacc ctgtatttcc caacatactt cgactggcgc 36180 ggccgtctgt acttccgcag ccggatcaac ccgcaaggca cagactttgt gaaggctgtg 36240 atccagtttg cgaacaagaa gccgctgggt aaacgtgggc tgtactggct gaaggtgcat 36300 gtggccacct gctacggctt cgacaagaag ctgccagact gccgggcggt gtgggtggac 36360 gagcacatgg cgcagatcaa ggacgctgtg gcccgacaca tcgactccga cttcttccgt 36420 gaggccgaca gcccgtggtg cttcttcgtg gccgcacggg agatgctgcg cgccatcgaa 36480 tccggcgacc ccgagtcgtt cgagacaggc gtgcccgtcg ccatggatgc tacttgcagc 36540 ggtatgcagc acctatcggc agtgctccgc gatcccgtgg gtggtatgtt cacgaacctg 36600 ctgttcaata acgggaccga gaaggaagac atctacgccg gtgttgcagc aattgctata 36660 tcccgactgc aggctgaccg ggacaatcct gagcaggccg cgtactggct gacccacggg 36720 gtgccccgca gcatggcaaa gcgcccagta atgacgtacg tgtacggcgg caccctgatg 36780 tcctgcgggg attacgtgct gctggacatg caggagcgcg ggctggaggc cttgccggag 36840 tacagcatgt cgaagctggc cgtgtacttg ggccggcatc tgcggcacgg tatcgagctg 36900 gcggtgccct ccgccgcgtc tgctatgcgg ttcctgcgtg cgcttgccgg catgatgccc 36960 cgcgatgagc ccatgcgctg ggtatcacca gcgggattcc ctgtgatcca gcattacgcg 37020 gccgaggagg ttgtgcggtt tgacctgcaa gggttggggg tcatggtgag catgacgcgc 37080 tttgacgata accgactgaa tcgctccaag tgtgtgaacg gcatcgcccc gaacttcgtg 37140 cacagccttg acagcgggca cttggtgcgt gtgctggccg cgtacaaggg cagccttgtg 37200 cctatccacg attcgtttgc cacgcaccca tcggatgtgg atgacatgca ctgggtgctg 37260 cgcgatgagt tcgtgaagat gtacgaggag tcggacccga tggacgcgtt ggtgcagggt 37320 gtgcagcaat tccgcgaaga ggtgatcgag cagcctgtaa ggggcacact agacatctcg 37380 aaggttcggg attctgtgtt cttcatgtgt taggaggtct tatggatata caacgtctgc 37440 gtgaacttta cgcagtaagc tctgatagcc catccggggt tgttatggca gcggataggc 37500 gccgcgggtc tggtaagaag ggccaacccg cattacatac cctgcggaac aacggctgcg 37560 ggggttttta ctatggcggc gcttatgtag aaaccacatc acaaggaaag gtgcgccacc 37620 ggacggtag accacgtaga tggcaacacg ttaaacaacg accccttgaa tctccgcgcc gctacagcgg 37740 cccagcagca ggccaacaaa ggcgtctcta ggcgcagcgt tacgggtctg cggggcgtgt 37800 actacaggcc agaggacagc gcagtacgcc cttacagggc aatgtgtaaa ggtaagtatt 37860 taggtatatt tcatacgcca gaggaggcgc atgccgcgta ccagcatgca gcggcggaag 37920 cctacggcga gtttaatagg gcgacacgat agtgtgggtt tgactctgca cttagtgtac 37980 agagagggat gacggcacag gatgcggtcc ttctctgtaa agtgagacgt acctaagagg 38040 tgaattgcga atgtcttctg atcagaagaa agtaacgttt tcagtagagc agattgattg 38100 gttggagcag cagttcccag agctgcagtt cctacccgag gcatccctag gggagcttca 38160 gcatgctcaa ggccagcgat cggttatcca ccgcatcaag caggtcagtc gggtggaggc 38220 acggaatgtc ccaataccag ttaaagtatc atgacatcgc cccagaaggg ttgatccgtc 38280 aggtagctga tatcgtctgg gtgcaccccg cgtacaacca tgcgcgagcg ctgtaccctg 38340 atcccgagag cttcactgag gctatgctgg ccgatctcca tggattccct gcacttgcag 38400 cgtattccgg ggattctcta gcggctgccc tgtcgttcac tgatcttgtc cgggacctgc 38460 acatgccggg cctaggcagc atcgtcggca gcagcatctg ccacccagaa tatcccgggg 38520 cagccggttt cctgctgagg gcgtacatcg ccgccctgcg tgctgagggt gccaagtggt 38580 accagatcag caagcggctc gatgccacaa cagtcctcac caaataccgg aggctccatg 38640 agtaaaaaaa tcaagagcgc tttcgatagc gtggccaagg tagcaacctt cgggttggca 38700 gacaccaaga agcttaccga tgtgttcacc cttggcggtg gcggcggcct gctcgatggt 38760 atcactggtc aagcggctat cgacgcggct aaggctcagg ccaagaccca agaaaccatt 38820 gcccagcaac aggccaccat cgcccagaac gctgctcagc tgtccgctgc caacgcggta 38880 gacaactcgg tgaacgtgat tgccggcggt actgccaacg ctgccgactc ccttggaggc 38940 gacttcaagc gcaagcggac gaattccatc gcgtcccaat tgggggtgta acatgaaggc 39000 accagtcaca tacgagagct tgttcaccaa gtaccgtgac gacagcgccg tactgcggaa 39060 cgagcagtat gcgctctgga gcatccccac gatctactcg gactcagatg tccgcacgga 39120 cgggaagcag cagagcgtac gccgcgactt ccagtccgct ggcgccatgc tggccaacca 39180 cttggcgagc aagatcgcat cgctgctgtt cccgtccact cagtcgttct tccgccttga 39240 ttcggcagtg aaccctgacg agatggcctc ggcactggcc gtggaccaga acgagctggc 39300 ctctgggctg gccacgctgg agaacactgc gtaccgccgc atcttcctcc gctcgtcgta 39360 ccatcagctc gtgcatgcca tgaagctgct gatctgcacg gggaactgcc tgctgtaccg 39420 cgatagcgcg aacacgaata tgcacgccta cagcatgcgc tcgtacgcca tcctgcggga 39480 tgggtcgggc aaggtgctgg atatcatcct gaaggagcgc accacagtcg ggcagctgcc 39540 tgaactcctg cagagcacgg cgtatgccgg gcgcgaggag tacgatagcg tgtgcctgta 39600 cacccgcatc aagcgtgagc gccgcaaagt caccgacgta ttcgtggtga cccagcaggc 39660 ggactcgcat gcgctggatg tgcgcgagga gtacccggag gcagtatgcc cgtacatccc 39720 cgtaacgtgg aacctcatca ccggtgagaa ctacggccgt ggcctcgtgg aggactacgc 39780 cggcgacttc gccaagctga gcgagctgtc cgaggccctg gccctgtacg agattgaggc 39840 atgccgcgtg ttgcatatgg ctgccccggg ctctcaggcc gacatcgatt cgatggcatc 39900 cgaggagatg ggcgcttggg tagcgggtga ccccaccaag gtgcaagcgt acgaggctgg 39960 cgactacaac aagattgtgg ccctgaccgc ggacctgacg caaatcttcc aacgacttgc 40020 gccggcgttc atgtacggcg ggaacgtgcg agatgccgag cgggtaaccg ctgaggagat 40080 ccgtacgcag gccgaggaag ccaaccagtc gctgggtggt gtgtacagcg tgatcgctga 40140 taacctgcac atcccactgg cccacatcct gtgtgccgag gtgaaccctg acttcgttgc 40200 cgaggtaatc gccggcggcc tgaccttgag tgtactcacc ggcgtcgccg ccttgggtcg 40260 cagctccgat gtgaataaac tcctgcaggt tgcacaggtg ttgggtgcca ccctcccagt 40320 attcactcag gtgagccagc ggctcgaccc tgagcgcgtc atctccaagg tgttcgaagg 40380 ctttggcctg aacatcgaag actattccca taccgcagaa gagttggaag ccaaacaggc 40440 cgctaacgct gcacccgtgg acactgccct gaccggtgcc gcagctgaca tccaaggagt 40500 cctgtaatgt ctgagaacca gacccaagat caagtaaacg agttcggcag caagatcagc 40560 cccaacgctg acgcgaatgt tgccctgaac gagaacggcc ctgatcaggg tactcttgct 40620 caaatccttg cagccatccg tggtaaggac gttgagacgc agccagctgc cgccgacaag 40680 ggcaacctcg gtgttgatgc tgatgccgac gagaagccac tgccaaccgc tgacgacgag 40740 gacttcaagt tcgagaccgg caacaaggcg ctggatgtgg ctgtatcctc gttccttcgc 40800 agcaccggtg caagcgatgc cgacatcgag cgcgcatgca agtccgctct ggagtatggt 40860 gaccctaaac tgatcgaccg cgccttcatt gctgagaagt tcggtgaccg cgcagcggac 40920 gccgtggctc tggccgaggc cgtaatcgag caggccgggg ttgagaaaca acgtaccgtc 40980 caagctgtgt acgatctcgc tggctcgaag gagcagtggg acaccgcact gggtgtgtac 41040 aagcagcacg cccccgctgg cctgcagaag gcgctgggga ttatgttcag ctcgggagac 41100 gccgcatccg tgaaggaagc agccggtctc gtgttggagt ttgccaaagg cagtggagtt 41160 ctccccgtgg gtgcggggca acgccaagtg gctggaggcg gtaccgctgc ctctgaaggc 41220 ctctcggctc aagaattcaa ggccgcactg agtaagctga acccgaacgc acgcagctac 41280 catcaggact atgagcgcct gttgaacatg cgtcgtgtcg gcaagcagct cggcatgtaa 41340 tctaatccaa ggagaatcat atggctaaca ccgcctacac tggcaacctg actcgcccgc 41400 actggggtgg tccgaactcc gacgctgaca tccatctgga ggtcttcgac ggtgccctcg 41460 acaccgcgtt ccagtacaac gcgttcttcc gtgccaacag caccttcatc tcggtgaacg 41520 atcgttcgaa caccgcccgc atcgaccgtc tgggcactgt gaccatcaag ggtcgtaagt 41580 ccggcgaggc gctcgatcct actccggtca agaacgacaa gctggtaatc accgtggaca 41640 ccgtgaccta cgcacggtcc ccgatcgatt accaagacga ctggaccgct ccggacttcc 41700 tgccggctat cggccagaac cacggtaccg agcacgccaa gctgttcgac caagcgcaca 41760 tcatccagct gatcaagtcc cgctcgtgga ttgccccggc tcacctgaag ccagccttct 41820 acgacggctt cgagaagacc gcccagctgg tgaccaacga cgctgctgcc tcgtctgacg 41880 ccatcgtcgc tgcccacctg agtggcatcg agaccctgat caaccgtgat ctgggtggct 41940 cgctgaacga gttcatcact ctggtgaccc cacgcgtgtt ctcgctgctc ctgcagaacg 42000 agaaactggt gaacgtccag tacagcgacg gtaacgcaaa ctacgcccag cgccgtgttg 42060 cgtacctgaa cggtacccgt ctggtcgaga ccgctcgctt cccgaacgct gtcatcacca 42120 accaccagtt gggcgctgcc ttcaacgtcg actccaccga catccaagta gagatgatcg 42180 tgtatcatcc gaagatgact ctggtgaccg ttgaggccaa ggcgcacacc tcccgcgtgt 42240 gggacgatga gctgaacttt gcgaacgtgc tcgactcgta cgccatgtac accatcggcc 42300 aacgccgccc agacactacc ttctcggtac gtctgctgcc tggcgcgtaa gacccttggg 42360 cccggttcgc cgggccctca ctatttagga gacctgccat gacactttta gaggcggtaa 42420 acacctgtct gtccgccctt ggcgaatcgc gggtcaccag caccacagta gtgcacccta 42480 cggtggacct cgtcctgagc accatcgcca tgaagcagcg ccagctgctg gagcgcgggt 42540 ggtggttcaa catgagccac gtcaccatgt acccatccac cacgggtgag atggagtacc 42600 ccgccaacgc cattgccatc gtcggtgatg agggcgaaat cttcgtgccg cgcggcggca 42660 tgctgttcga tatggacaac aacacccagt atttcacgga gcccaagagc atgaccgtgg 42720 cgtacaacct cgacttcgag gaccttcccg agtgtgctgc aaacgtcgtt atgacccgcg 42780 cggcgcagga agtgtattcg ggtgaccttg gcccggacgc tgctgtgcag cgcctgcagg 42840 gccttgagat catgagcttt gccgagatgg agttgcagca catgcgcaac cgccggtaca 42900 gcaccaaaca acgtcgcgga tggggtcgtc tcgtccgtgc cctgcgcggc taggaggcac 42960 tatggcggct atcgacggcg caaccaaatc gttaatgcag ggcgtgtccc agcaagtacc 43020 ccgcgaacgt ctcgacggcc aggtctccct tcaggtgaac atgctgtcgg acgccgttga 43080 aggcatgcgc cgccggcccg gtatgcggta cctgaccagc aacatcttcc cgggcggcgc 43140 ttggaacaaa gagcaggtgt tcgccacctc cgtggacatt ggggacgagc cccaccacat 43200 cctgatcaac accgccaccg gcgttgtgaa cgtgtacaac agcaatatgt cgctggtggc 43260 tactgacgac ttcttctacc tgctggcgcc taccgctgcc agtattcagg tagcggcatt 43320 acgtgggtac ctgtacatct gcaacactga gcagttccca gtcaagaacg tgactaatgc 43380 cggcaagcag aaccctgaca agacgggttt cttctttgtg aagacggggc agtacagcaa 43440 gatttacaac gtcactctga acgtgaacgg ggtgcagtac gcctcatcgt acactacacc 43500 gaacggatcc gcccccggcg atgccgccct gagcacgcct gagtacgtag ctggcaagct 43560 ggtggacggt attgtggcac acgccattcc gggcctctcc tttgccatcg cgggctcgta 43620 cgtgtacctt gtcaccacgg gtgctggggc catcactgtg acctcagatt ccggcacaaa 43680 cctcatcagc gcctcgaacc agtcccatgt caccctgact actgagctcc ctgcccggat 43740 gcccgcagct gccaacggca tgctggtaag cgtgggcgcc aacccatcgc tatccgtgtg 43800 gtaccgcttt gacttcgtga acaacgcgtg ggttgagtcg ggggcttgga acagcgccga 43860 cttcctgacg aatatgccca tccggttgaa gctggataac tcctacgtga tggaccagcc 43920 aaactatgag gggcgtttgt cgggtaacga caacacgaac gaggaccccg ccttccttac 43980 ggacggcgtg accggtctga gcgtattcca aggccgtctg gtaatcctaa gcggggcaac 44040 ggtgtgtatg agcgctgcgg gcaaacccct gcgctggtac cgtagcacca ctacggaact 44100 cctcgttgcc gatcccatca gcatcttctc aggctcggct accactgcca acttcacaca 44160 cgccatccag tttaacaagg acttgttact gtttgccaag acctgccaag cggtagtgcc 44220 ctcgggtaac tctgtagtgg cgcccagtac ggcccagatc gtcatcacca gtgcgtacaa 44280 cacaactagc aaggtgctgc ctatcgtggc agggcggtcc ctaatgtact tcgcaccccg 44340 cagcgagaag ttcgccagcg tcctagagat ggtgccgagt aacaccacgg actcgcaata 44400 caccaccaac gacgtaactg cacacatccc acagtacatg ccggggacca ttcggcaggc 44460 tacggctagc accaccagca acagccttgt gcttgtcgct gacggggatg cccgcaccct 44520 gtttgtgcac gagtacttgt ggggtgggga tgagaaactg cagtctgcat ggcataactg 44580 gaccgcaccg tatgacatcg tgtgtacttg gtttgtccgc gacacaatct acgtcggtat 44640 gttagtagac ggcgacctcg caattgtcac gatcgactca caggcaggcg acacagccaa 44700 cggcctgtac cgcccgttct cggacatgtt catcggcact gcagtcgtcg gcggcacctt 44760 tacggttccc acggcgctgc gcgctgcata cacagccgga gccgagttgc agttgaccta 44820 caccaccggc gcagttgctg gcgaggcagt aggcttcagc atcaacacag ccacttgggt 44880 tggtacagcg gtgcgtaacg tccctgacgg cacgtacttc ctaggcctca agtaccgcag 44940 cacgctgtca cctacaccac cgctactgcg cgatcagaac ggtgtggtga ttggaaccgc 45000 aggcacatcc ctgatccggt acgaggcctc cctgaagaac acgggtgatt tccatgtggt 45060 tgtgcaacgg cacgacgaga ctacctcgga aggggtatac tccggcctta catacagctc 45120 gcaggacttg atccccggtg ggcccctgta cgccaagagt agccggtgca tcatcccagt 45180 gcgtttgaac cccgacgcct ctagcgtgat catgtacgca gaagacgatc acgacctcgg 45240 cgtgcttacc cttgagcacg ttatgcagta ccaccctcga cgacggagaa tgtaaatgat 45300 ctggatggcc gcagcagcgg cgcttcaggc agtgacccag ttgggggctg cccgggggca 45360 ggccgctatc gacaaagcaa ataaccgcgc cattgaggcg tacaacaaac aggtggc 45417 <210> 2 <211> 2730 <212> DNA <213> Pseudomonas putida phage <400> 2 atgccagtac gccaagatgt acaaggcggc ccgcgggctg ccggtcttga gggttatcag 60 agcccgggag ccgcccgtgc gcagttccaa ggcccagacc tcagcggcgt acagcgcgat 120 gccgcagacc aagccaactt ctggacccgg ttgatcggcc gtggggcgaa cgccctgcag 180 gctatggacg agaaagaccg ggcccgggcc tatttagagg gtcagcaaga cagcgccatg 240 ggcaaggccc gcgctgaagt gtatggctca atgcagtccg actacgagca aggctataac 300 cgcgccacca ttggcagcca gttggcgaag taccagctgg gcgtgcagca agccgccatc 360 gaacatgtga actcgggcaa gaccccagag gagttccagc agttcgtgga ggaggagacg 420 aactccctgc tgaacgcagc cggcactcaa ggccttgatc tgaacgagaa ggactggcag 480 gcgtggctga ccggcgtgca gagcacccgg gccaccgccg ctgatctgtt ccagaccaag 540 agcgtagagc gtgcccagta catgaagggg caggccatcg ccgccgaggg caacgccagc 600 attgctacct tcatggctgc cgatgaggcg ggtaacccac tgcaggcgct gggcgggatc 660 acctcccaca ttgcccgcgt gtactccgac aacaccttgt cgcctcagca aaaggacggg 720 gcccttgcgg acttcgctgg ccaactcatg gcaagcgcta agtcgtccgc cgccgttgag 780 gcgctgggct cgtacttcca agagtcggcc gagttcaaat ccctgcctac tcaggtacag 840 cgaggcgc gcggtgtact cgtacgtgtc gaacgtgcgc gctgtggcca acccggatga gctggagcgc 960 cagttcccca tggcgtcctt tattagcacc ctgaacgagg cccagcgggc tcaccgcatc 1020 actccggccc agatgtacgg tatggtggag gaggagaaca ctcgccgcct gaagctcggc 1080 aaggctgccg cattccagca gaacctgctg cacggcacca ccatgtccga tatggcagct 1140 ggtaccggtc agactctcgg caagatcaag actgccctca cgcaggtata cgccaccagc 1200 aacggtggct acagcggcgg cgggctggcg ctcatgcagc gtggtctgca gagtggggcg 1260 caggacatca cagctgtggg tatcgagatg ctgcagcaag acgcacagtc cctcggcagc 1320 atcgacagcc ggaacctgaa gcgcgatgct gacgggcagg ccctgtaccc ggctaccgtg 1380 gtgaactcgc tgacgaacct gaagcgtgcg tacgacgctg cacaacgagc cggcaacaac 1440 gtgcaggctt ctcagctcat tgctgggctg cccgacgctg tagcctatgg catccgccaa 1500 gccagcgacg agaatagcat tgcggacgtt gtgtaccgcc gggccgatga tatcgcctcc 1560 gggcgcgtgg tctccctgcc ggctgccatg cccaaggaaa tgctcgccac tgtggacgat 1620 gtatccgccg gcatgttcga cacgaacctg acccagaagg gcgcggctcg gaacatcctc 1680 ggcatccagt cgtacgtgtt caccagcaag gaagatgccc gggcgcagga agcccgcttg 1740 gcgcaggtga acggtgccct cggtgaggag tacacctcac tgtaccagca aggccgtctg 1800 ccagcgctcg ccggcgacga cctgaagaac tggctggtgg gtcgtgtggc tgcccgtacc 1860 gtgcgtgtag acgatggcac cgacaacggc acactgctga tcctgcctaa cgtggccaac 1920 cgcgcccaga cctttggcag tgaggacaac aacatcatcg gccaaggcct gaaggactct 1980 gttgacgagt tccgtgcagc caatcccggc gctgtatccg tccagatgcg gtacgacccc 2040 atggctggcg agatcgtgtt cagcggcact gacaaggaca acacggtgct caccacttcg 2100 tacgccgtgc cggtcgatca ggttgtacgc caagtgcgtg cagttgagac tcgcctgacc 2160 gcgaacggta atggtaacag catgggctcg ctcgctgtgc ccggcgccgg gtttgtgcag 2220 tttaatacgg ccaacgccta cggaatcgag ccgcaagtgt atcaaaatgc agttacccag 2280 ctggtctcat acgagggcta caccgacacc aaaggcttca gcatcctggc cacccaccca 2340 accacgggtg cggccctgaa cgaggccaag tacgtgaagc agcccaccga cacgccacag 2400 gtcgctgcga acaagctgca actgtacatc caagacaagg tgcttccgcc tgtcatggct 2460 gagttgccgc agttcaacgc cctgcctgag cacatccgtc agcaggtgtt ccagcagctg 2520 gtggagacga cgtatcacgc cggtaacgcc aaggccttcg gggacatcct gaagaaggcg 2580 ctggatggtg acacactggg ggcctaccaa gacttccgcg agtccgccct gtacaaggac 2640 gctgggccta agtcccgccg caaccgtgac cgactgcaaa cccttgacgc tgtatcccaa 2700 tacagccgcg tgcaacgcac cggctactga 2730 <210> 3 <211> 3795 <212> DNA <213> Pseudomonas putida phage <400> 3 atgagcatga tccgtttacg ccctgacgaa gaccttgctg tcgcaccggg caccgccccg 60 actgtcggcg gcgggttcgt ctccggcgct gagaacagct tactggaacc cggtacagca 120 aacacctcgg cgctgaaagt agcgccgacc tcttctgcag agagtgccga gggcgctctc 180 cgcgaaacca tcgcagccaa ggcgctgcgt ggtatcgagt acatgcaaat cccaagcgag 240 gaggggttcg accctgcagc cgcgctgggc gacaaactcg gcgccttcac tgaggaagag 300 ctggagttcc ttggcgatgc ccgctccagc accgaactgg cccagcgcct cggtcaagtg 360 acccagaccc gccagaacct gcaggacatg gccgccaacc ctgtaaccgc cttcgccgcc 420 tcgatgctgg acatcgacgc tgtgatcggc ctcggggtag ctggtgctgc tacgcgcgcg 480 ggccgtaccg cgcgtctggt tgccggcctg gccgctaaca ctgcggtact gggcgctgca 540 tccgagggcg gtgagatcac cccgctggat gtcatcggca catcgctggt cgtagcgctg 600 ggcgcaatcc ccaccgtacg ccgtgcagca accgttgccg aggacgcggc tcaggacctc 660 ccaacaggcg cgcgtagcgt tcctgacgtt gaggaggtag tacccccacg ccccgctgct 720 gaagtcgctc cagatgtcgt ccctgacgct caggtggccc cttcccgcat ccctgaccca 780 gactatgaag tccctacccc tgacatgacg actgtacgcc cgtacgtcga agtgaatcgg 840 gtcaagggtc aagccaccat ccgcaccagt actgcgaacc ttgtgggcgc cacactggcc 900 aatgccagcg acctgcccga gggtacccgc cttctgggtg tagccttgta cgatagcatg 960 aagctggacg gcgacgtgcc cctgatcatc acccgtggca agggtcgatc gaacgtccgc 1020 ctgtggccag acggccgcct gcagaccacc atgcgcacag ccgagggcgg ggcacctacc 1080 ctgactgagg ccgtgcagaa catgagcacg tacgagaaga ccgttaacct gcacgaggca 1140 gcacacgcca agaccatcac agcgattgcc ctgttccaac agggtaagct ctcggctggt 1200 ccagtgttcg acgcagtgca gcagatcgat caaatccgca acgtcgtgcg gggcaacatc 1260 agtaccccaa ccctgcgtga gggcatcagc acttccgact ggaagaacaa cgtgctgtac 1320 ggactcagca acacccacga gttcgtgtcc cagctgttca acagcgagtc cttccgcaag 1380 gtactgcacc agatccctat gcccggtgag ccccgctcag tgtggtctag cttggtgcag 1440 aaggttgtga ccgccttcac tggcaagggt cctaccgaca ccgcgctgac ccgcttggtg 1500 gctgcattcg aagacctatt gcaacagccc tctgtgactg acgcgcagtt cgtgaaggcg 1560 tacaacaagg ccgacgtgcc gcgtacccaa tcggccgcgc tggctaactc cccagacatc 1620 cagaccatgt tcacccgcac cgggaactcg ttgaatcagg ccttcgcctt gttcgacaag 1680 attcgcgcca tcggcccacg ggccagcacc cttgcggaac aactggttgt cgatgccact 1740 gggaactcgg ctacgtctgc tgcgcattac gcccgcactg cccacttggc tgccaacgtg 1800 gccgctgcgc aggttgatgc tgctgtgcgt caggccctgt ccgcccaagg ctggactatg 1860 ttctctcgcc tgcgcaaccc agctgcattc cgggctgcac agcaggagct gtccggcaag 1920 gtgtacgacg ccctgtccga taaccatgcc cgcttcatgt cgggcggcac cgtgaaccct 1980 catccagacg cagccgtgca gcgcatcgtc gatgccttcg ccagctcccg ctgggctgag 2040 gaccagctgg cccgcatcaa ggcctctggc atgaccggtg ccgacaccat cgaagcgtct 2100 ccgtactact tgccacgccg ccacagcggt aacaaggtga gcgacttcct ccgtgccaac 2160 ccgggcgtta ctcgggcgga cattgaggcc atgtacggtg agcagttcaa gttaatgttt 2220 gcagaccgcg gtatccgccc ggacactgcc cgcgcgctgg gtcgtcagat gctgcggaac 2280 atggatgagc gagccgccgg cgtgcaaggc taccgccagc acatcgcagg tatgaccaac 2340 gacgacatcg agttcgctat gcgcaacgcc ggtatcgacg aggatcagat caacatgttc 2400 ttgaacactg tgcagaaagc gggcgagcaa tccaacaccg tgcggaacct ccgccggcgc 2460 gtggactttg acatgacccg tgagtacgtg accaagagcg gcgatgtgat catgccgcag 2520 atgttcgtgg acaaggatgt gctgggcctg atggaaggct acagccgcac catgagtgga 2580 cgtattgggc tggccaaagc cggtttccct gacgtgcgta cgctcgccgg cgcagtcgat 2640 gaggctgccg ctgagggcgc agacccacgc gcagcgcgag ccacgttgga caacactgtg 2700 aaccgactgc tgggctaccc cactggggaa gacgtgcctg atatcctccg cagctttgct 2760 gtggcgtctg gtgcggtgca gctggccaac tctggtatct accagctggc agacactgcc 2820 ctgatgatca aggagttcgg tattgccaag gtactgcggt ccctgagctc cacagagtgg 2880 ggccgtgacg ctttgaagct ggcacaggac ccaacctacg gctctcgcct ccgcgacgtt 2940 ctggaagcac gcaacgtgct ctcaggccgg taccggacag tgatgaccca cctcgacgac 3000 aacaccgata tcggaaacct cggcatgctg caccagaaca tccagcagct gggtcaagtg 3060 acccgcttcg ccaacggcat ggagttcgtg cgccgcgggc agtcgaagat gatggccggg 3120 ctggtaggtg acaccgtgga tgacgccatc cgtggcaacg acgcggcatt cgagtccctg 3180 cagcgctttg gcatgacgcc tgagctgcgt gcccgggcta aggccgcgat ggatgccgac 3240 cctgacatgc ggatgtggcc agacagcctg cgcctcgaca tggagactgt ggcgcacaac 3300 atggctgacg cccttgtcat ggagaaccgg ttgggtgagc ttcctgcttg gatgcagttc 3360 agcgccctcg gcaagttcat cctgccgtac atgaacttcg tggctggtac ttggaacaag 3420 atcctgcgcc gcacgtacgc caacgacggc cttgccggcg tggctatgat gttcgcgtat 3480 caactcccgc ttacaaccct ctcgtcaact gtagcgcttg cgcagggcaa caaggagatc 3540 actcctcaga ccctggccgt gaacgtgctg acccaaatgc cgctgatgtc gtggctcggg 3600 tacgctgtga acatgatgac tcaaggcccg acgaacagca ttgctgctct tgggttggtc 3660 gataaggcat actccgctac cgcgtcactg gtgcgtggcg agcctgatcc agagcaaatc 3720 atccgcgcaa cccctttcct cgggatcatc cccggtatcc gcatcatggc ctcggccttg 3780 ggcgaggacg aataa 3795 <210> 4 <211> 2175 <212> DNA <213> Pseudomonas putida phage <400> 4 ggcagcttag gaatacttcg agcagtcgga tatctcggtt ttcttggact tgagtatcgc gccacttatc 120 gaaggcactg actacgcgtg ggccaacgcc acccgcattg acttcctgac aggccctgtg 180 gccgcaggtg tggcggtgac attggttcga aacaccgaga acgaggcaat gctgaacatc 240 tacgatggcg gcgccccgtt caaccgattc acccttgacg agaacttctt gcagctgctc 300 cggctgtcgc aagagttctc cgagggctta ggcttgaccg gactgcggca ggccttggac 360 atgcacggct accgcatctt caaccttggt aacccggtga atgcakhga tgctaccacc 420 aaggcgtacg tggacctgct gatccagacc cttgtggaat cgggtaccgg cccaatcggg 480 gccgcctcga atgtcgtgta catcgaccct gaaggccagc taaagaacct gcagcagctg 540 tctggtctcg atggtgcgga cctgatcggc tggaatggca cgggcctggg tccgttcctt 600 gaagccggct actacgagcc gcaagggctg gaccttaccg gtgttgtgga cgagtctgcg 660 gtaatcaacg ccgcgctagt gaagtacaag cgcgtgcgtc tcccggcagg caccattaag 720 gctaacgtgc tcgtgccatc cggctgtacc ctgcggggcg ccggtaagac ccggctgaac 780 cgtagcacca aggtatgggc cgccggtggc accaccatta tcggctctgt agtggcgtcg 840 aaccagatca actggactgt caccgacatg aacatcgacg gcttcgccgg cggtaacgct 900 tttggtgctc agggcccatc cacagagttc gggtacatcg gtcgagtggc aatgcgcggt 960 agtgaccaca acatgatcct tgagcagaac ggctctgatc ttgcaggccg cttcggcggc 1020 aatatcctcg tcgaggactg cgatgcgtat gatggcccta acgggtttgc tgtgaagatg 1080 cggaacgtga ccttccgccg gtgctacgcg tacgacatca cagtgcaggg ctttgtggct 1140 gtgtctgaca acatcaacgg cccggctatc tactcgcgcg cgcagaacgt gcgctttgag 1200 gattgtggcg gcgacagctg caaccaaggc tttgtggtgt attcccgcga cttctaccac 1260 gggaatttcc ccggcagctg ggtcgccacg gtgagtcccg ctaccgacat ctactgggac 1320 gt; accttcgcca ttgtgttcaa caacgagatc gagattggcg gcgggcacta ccagcactcc 1440 ccgctgtttg gggtgcggtt cgactgggcg gcacgtccaa aggtaaccgg tggccacttc 1500 tccgggtgcc cgaacccgat cgtgcgcggc acgaacgtgg tagacctcaa gattggtgat 1560 ggggtgacgc gcttcggtgc catcaacccg ggcatcctgt ctgagcgcat tatcgagagc 1620 tcaaatacca gtgccgtgaa catccagaat gtacgccaag tgctggtgtt ccagaacacc 1680 gtgccgaccg tggttacatc cctcgtcacg cctgatacca accacaccat tgacgttgtg 1740 attgacgacg gcgttacctc actacagctg gcgggcatcc tgctcaccgg tcggggcagc 1800 cgggcgcgct tccagtggtc ggcagatacc agcacttggt tgtgcctgat gagtggtagt 1860 gtgctgccac cgggcgagat tgcggttccg tacgccttga gcatgtctgc cgattggcgc 1920 gccctggcaa actccatcgc catgaacggt aacatcaacc tgctggacgt acctgtggcg 1980 aacatcgccc cgggcactgt agcccacctg cgcatcgtga acgttgccgg cggctcacgc 2040 agtgtgttca actggtcagg tgtaacgtgg ggtgccatca cccccgtggc tgccattgcg 2100 gccggtgcca ctgtcctcat gaccctgtac cacaatggaa gcacttggtt agtcaagagt 2160 gcccagacgt tctaa 2175 <210> 5 <211> 294 <212> DNA <213> Pseudomonas putida phage <400> 5 atggctaagt cagcaagcaa gagcctgctg aacgagctgc acgagatgct ggcgcaagtc 60 ttcattgacg acctgcgcca gtcgcgggag gagggcattc ccctccccgc cgctaacctc 120 ggtgtgattc ggcagttctt gaaggataac gatgtgagcg cgagtatcga tgctgacgac 180 atggcgaagc tgcgtgacac cttcgcagac gagttggccg agcgccgcgc actgaacaaa 240 gcccgattgc agcagacact gcaggaggac gatatcaacg agttgcttgg gtaa 294 <210> 6 <211> 1815 <212> DNA <213> Pseudomonas putida phage <400> 6 atgattcgag cgcacacact agcgcgcctg cggctgctcg tcgagcgcac gaacaagtgg 60 aaggacaaca gcaacgccat ccccgctgag gagcgagttg atctcgccct catgttcggg 120 gccacattcc cacgtttccg ggagttcgct gagctaggga tgaagttcct cggcttcgag 180 ctcacggaga tgcaaggcga cattgcagac tacatgcagg atggcccacg caacagcatg 240 gtggccgctc agcgcggtga ggccaagagc acccttgcgg ccctgttcgc tgtgtggtcg 300 ctcatccggg accagagcat gcgcatcctg attatctcgg gcggcgagaa gcaggcttcg 360 gcgtagcca tcctgatcat ccgcatgatt gagacttggg gtttactgtg ctggctccgg 420 ccagacaaga cccgtggcga ccgtacctcg tacgagagct acgacgtgca ctgcgacctg 480 aagccgctgg acaagtccgc ctccgtggcg tgcgtgggca tcaccgcgca gctgcagggt 540 aagcgggccg acctgctcat tcccgatgac atcgagacca cgaacaacgg cttgaccgct 600 accagccgcg aactgctgct actgcgctcc cgcgacttcg cggccatcaa cactcacggc 660 aagaccctgt acctcggcac gccgcagacc aaggactcca tctacaagac cctcgctcga 720 cggggattcg aggtgcgtgt atggccgggc cgcgtgccca ctgtggagga ggctgagcgc 780 tacggcgaca ccctcgcccc atacgtggct gccatgttcg agacccatgc ccgcactggg 840 ttcgggctgg acggcacccg tggcgaggtc acagacccgg gccggtacac tgaggaggac 900 ctgcagagca aggagctgga ctttggcccc gagggcttcc agctgcagta catgctcgac 960 acgtacctcg tcgatgccat gcgcacgcgc gtgaagctct ccgatgcgat tgtagcatcg 1020 ttgggcaccg atgctgcccc tgataccctg tactacgccg caacgccaca gcaccgcctg 1080 caggagctcc cagaggctat ccacggggag gtcatgtacc gggcggctgg cgccggcgag 1140 ctcatgctgc cgtaccagca caagatcctg atggtggacc cggccggttg cggtggggac 1200 gaagtggcct tcgccgccgg cggggcgctg aattcgtaca tccacctgtt cggtgtgggc 1260 ggtctgcagg gcggtatggt cgaggagaac tgcaatgcct tgatcgatat ggctgaagaa 1320 atgggcatca ccgacatcgt gcttgaagcg aacatgggcc acggcaccgc atcactgctg 1380 ctgatgaacg cccttgccaa gcgcaagatc acgaacatcg gggtgcgcga catctacgcc 1440 accacccaga aagaacgccg catcattgac acccttggcc ctgtgttccg ccgacacaag 1500 ttcgtcatgc acgagcgggc catagagatg gacgaggaac acctgcaagc gtacagccgc 1560 gagaagcgcc gcctgtacag cctactgttc cagctgcaga acatcacgta cgaccgcggc 1620 tctctggcca aggacgaccg tgccgacgca gtagctcacc tcgtgaacga gctcaaagga 1680 tacctcgcgg tggacgagga gaaggaagca gagaagttgc aagccaaagc ggcccgggag 1740 ttcattgaca accccatggg ctacaccaaa gggatgggcc gacgccccag tggcacccga 1800 tcccgtctac gctaa 1815 <210> 7 <211> 543 <212> DNA <213> Pseudomonas putida phage <400> 7 atgagcctaa agactaagat cgtcacggcc ctagcagcga gcacgctgct ggggtccgcc 60 ctgcttacca gcctgaccag ccttgagggc accgagtaca ccgcgtactt cgacagcgcc 120 ggcgtcctca ccatctgcag cggcgacacc acggacgtca ggaagggcca gagagcctct 180 cctagcgagt gcaagcaaag gctgaaggat ggtattgccc agcatgcgaa cgcgctccgt 240 gggctgcctg agagcctccc agacacggtt gtactaggtg ctgtgagcaa cgtgttcaac 300 atcggggagt acggctttaa ctcgtcctcc cagaagaagt gcttggcccg gggcgactac 360 gcctgtgcta aggcagcaac cctgcgctgg cggtacatca ccgtgaacgg caggaagttc 420 gactgcagca cccggggtaa ccgcgtgtgc tggggcctgt gggagcgccg ccagtgggaa 480 gt; tag 543 <210> 8 <211> 951 <212> DNA <213> Pseudomonas putida phage <400> 8 atgacgccat caacgcactg ggagtattgg gatgaacaac aaggtatcac tggccgcatc 60 cgtaatgtgc ggcctgacgt tgggtgccct catcgttgcg gctatcacgg gcggcttatg 120 atcccccggg cggagtggct gaccgcagcc caagccctcc cgattggtgg caagcgcagg 180 atcaagcacg gcttcgaggc caccgccgcc atggatgtgt ggaacaacga agatagctgg 240 tcctgctact gccatcgctg ccacgagggc gggcgagtgc cgaaggaaca tcagcgggtg 300 cggcgcgtca tggtcgagcc cgaccgcgta gcgcctgtac ctgcaacggt aattcgcttg 360 gcagatgcga gccgctggga acagcagcgg atttgggaat tactgtgccg caagggttgc 420 ccgccgggcg taatccccga ggaggtttta tggtacgaaa gatcagtgga tcgtctgatg 480 ctgctatcag acgacgtggc gctcgggagg gcgctggacg gcaggcggat gccgaagtgg 540 ttaccgtatg gggcgtggcg gggcaagccg atgatgtggg cgactcgtac cagtgccgag 600 gcaacggaac ctgcggactc tgcgccgctt gggtcgctga tcctgaccga ggacgcgctc 660 tctgcgtaca aagtggccaa ggccatcgac acctatgcgc ccagaagctc gctggacgta 720 tgcgcgaccc tcgggactag catcaccgac cggttcctac cgtacacact gggccggcct 780 gctgtgctgt gtatgtacga cggcgatgcc gccgggctgc gtgggtacag ggccatgcgt 840 caacgtctgg ctgtgtggca gcagcccgtg ttggacctac gcccagagca gggcgacccg 900 aagaacaacg acctagcatc tatagcggag gcactgagta agtggatttg a 951 <210> 9 <211> 1197 <212> DNA <213> Pseudomonas putida phage <400> 9 gtgcccgact ctctgctggg ccaagaaacc gtgagcatgc tagcgtggta cgctgcgtac 60 ttcaacgcat tccccgacaa ggactacgtg gataccgacg tactgcgctc gctgttcacc 120 ttgaaggtgg gcagcaccgc cacgccagag cagctggccg tgatgcgagc gctcatgaac 180 aaactcgacg agccagtcga tcccgaggta atcgacggca tcaccagtca gctgtacgag 240 cgcgacttcg ccggccgtgc cgccgccctg ctgaaccagt acgagaacgg tggcgaggtg 300 gacatcacgt acgagctgaa tcgcatggcc agcgagaaca tgcgccggct cagtgcggca 360 gccccgagtt cgtacattga caccccgatc gaggatatcc tcgcagactt cgggcaggac 420 cagcgggctg agttgcctac cacagccctg aaggagacca ttgcaggcct tcagggcggg 480 gacagtgtgg ctattgcggg gcgcccggac aagggcaaga cgagcttcct agcggcctct 540 ctgactggtt tcgctaagca gctgaagggg ctcggtctgg acggacgccc gctgttgtgg 600 ctgaacaacg agggctcggg caagcggatc atcccgcgaa tctaccaagc ggcgctgaac 660 atcacgttcg agcagatgct gcagatgtcg aacgccggca cgctgcgcga tgcgtaccag 720 aaggcggtgg atgggcagga gattcgcgtg aaggattgcc acggcagcac gctcggacag 780 cttgagcaga tcatcgaaga gatgaacccg tgcgctgtgg tattcgacat gctcgggaac 840 ttccgcatgc ctagcacggg cggcgggaac aagaccgacg cactggagca gatgtggcag 900 gaggtgcggg agatggctgt gcgccacgac ttcgtggcca tgccaaccat ccagatcagt 960 gccgacggcg acaacatgct gttcccgccg tacagcgcac tcaaggatag caagaccggc 1020 gtgcagggcg cgtgcgatgt gatcctgatg atgggcgcat tcaacagtgt ggacttggac 1080 aacgtgcggg gattcagcac gcccaagaac aagcgccaga tgccgggcaa accaagcaac 1140 gtacagagtc aggtgttctt cgatgcaccg acttgccaat tcctagatgg agaatag 1197 <210> 10 <211> 963 <212> DNA <213> Pseudomonas putida phage <400> 10 atgggcgcca agagagagcg cgtgctgcca gtggactgcc accgaccggt gaactggaac 60 gaggcagcag tgcagcgcgt cattgataac gtcgggtatg tgtacgccca gaccaagaag 120 gatggcatcc gattccatgc catgatcgat gctaacgatc gggtgcgtgt gctgacccgt 180 gagggcatcg agatactgtc ccttgacctg cacaaggaac gcctccgtgg gctcctgcaa 240 gccctgccca agcagtttgc gctggatggt gaggcggtag taccgggtat cccgttcgaa 300 gaagccagcg gcatcctacg gcgccacaag gccatcccgc tggaggactc cgtagagttc 360 tgggtatggg acgcgctgcc cgttacggaa ctgttggggt acgaggccgg gactgcaaca 420 ctggacgagc gtatcgacac gctggtgcag gcattcggca gcacgccgga gccgcaggcg 480 cagatcgtgc acatggaacc ggtgagcagc atggaggaac tgtacctgct gttcgatgcc 540 caacgtgagc tgggcgagga gggtctggtg gtcaaggacc cgagccttgc accgcgcaat 600 ggcaaggtca gcggcgcatg gaagctcaag ccgagcgaca cctgtgacgg ccgcatcacc 660 ggtgtgctct ggggtacgcc cgggctgggc aatgccggca agatcatcgg cttcaccgtt 720 gaactggaga acgggaacct atgcgatgtg gatggcctta cccaagcagc catggctgaa 780 tacaccgttc aagttgagaa ctgggcgcac tgtcaatccc caccagaata cgtatcgccg 840 cacccattcg ttgggcggta cgtagaggta agctacatgg agatgaccgc caagggcagc 900 ctgcgacacc cgaagttcaa gaccttccgg gatctggatt attgccccgg atggaaatgt 960 taa 963 <210> 11 <211> 2355 <212> DNA <213> Pseudomonas putida phage <400> 11 atgtacaaga tattcatcac tgacttagag actgagaaca agccttggta cggtcagata 60 gcatcaccat tcaaccctga gaactacatt gtagcccctg ggtggcgagt ggacaccgtg 120 catgacgatg gctctgtcga ggtaggtgag acgaagtgga ggtacttcgc atccgctgag 180 gaggcagacg ccggggccga ctggttcgac gagctggagg ggtgcgccat catggtggca 240 cacaacagca tgttcgagca gaagtggttc ctgagcaagt accgggagcg cttcgaggcg 300 ttcctgaagg ccggcggtag gctagcatgt acccaaatgg ctgagtacct aatcacggct 360 cagcaggaac tgtacccatc cttggacgag actgccgtga agtacggcgg tacgcacaag 420 gtagacgggg taaagattct gtgggagcag ggctacctca ccagccagat cgacaaggac 480 ttgctgatcg aatacctcgc cggcccgtcc ggcgatatcg acaacactgc gctcacgttc 540 tacggccagc aggagaagct ggcagagatg ggcatgaccg acatgtactg ggagcgctgc 600 gactctaagc tggcattcgg ttactgtgag tggttcggcc tgcacgttga tccagaggta 660 gctgagcgca accgcgcagc acaggaggcg gagatcgcag agatgcgggc gaagctgaac 720 gagctgctgc ccaaggacat gcccgactgg atcgagttca gttggacgag ccggtacgat 780 gtgtcggccc tgatctatgg gggcccggtg aagggtcgct tcaaggggcc gtacgacccg 840 ccccagtacg tcaaggccga ctacgtgaag actacggacg gtgcaacctt cctgttggag 900 gagatggata cggtcacgct ggacgccggc gcggactggg agcggtacaa gtcgggcaag 960 aacaagggtc aggtcaaggt gttccgcgag gacaccgacg aggagaagct gaagtggcac 1020 gagggttcgt accttatgcc cgggctcgta gacctgaagt ccctgcccga tgtgatcagc 1080 agcaagtacc ttggcaagcg cgccgagttc cgcggtgccc agaccctgtg cgatggcgtg 1140 accccggtat acagcaccgc tgaggaagcg ctgaagggct tgaagaacta cgtgcctgag 1200 gtcaagatca tggttgatct ggccaaactg gagaaggata ccggtacgta ctatctgcgc 1260 acggagtacg atgctgatgg gaatgtcaag gaagtcaagg gcatgctcca gtacgttcag 1320 aaggaggaca acatcgtcca ccacagcctg aacaccgagg cgaccgtgac cacccggctc 1380 agcagctccc gcccgaacct gcagaacctg ccacgagcag acgaggacgc agacggggag 1440 gcgaagtccc gcgtgaagga aatgttcacc agccggttcg gcccgcaggg caggatcatc 1500 gaggtggact acagtgcgct ggaagtcgtg atgctgtgcg ccatgaccaa ggacatggac 1560 ctgctgaagc tcctgcaggc tggtaccgac atgcactgct accgtttggc gttccagctg 1620 ggcgagccat acgaggaggt gctggccaag gtcaaggatg agagtcaccc tgagcacggg 1680 aagtacaagg ccctgcgctc gaacatcaag ccgctgagct ttgctgacca gtacggcgca 1740 tcagcagcgg ggctggcgtt caacactggc tgctctctgg agttcgctga gcagttccaa 1800 gagaacgagg cgaagctgtt cccgatcagc cgtgggtacc gtcaggtgat catcgacgag 1860 gtgaaccgca ccggcagcct gccgggcagc atccaccgcg agatgtacga caacggccag 1920 tggggcgtgt accgtcgcgg gtacttcacc gggccgagca ccactcggta cagcttccgc 1980 cagcagcgca cttgggacaa ggagctgcgg caggaggtga tggcgtacaa gccaacccag 2040 atggcgaact acccgttcca aggcgaggcc gggttcatga tgtcgtccag catgggccgc 2100 atctgccgct ggctgatctc cgtggactgg tttgacaatc aggtatgctt gatcaacaac 2160 gtgcacgacg cagcgtatct cgacgcagca aacgaggagg taggccgggt ggcagcgctt 2220 ggcaccaaga gaatcatgga ggacgcacca cgctatctca ccgagatgtg gccggcgttc 2280 gacatggccg atgtaccctt cccggcggct gctgagatgg gcgcgaacat gatggagaaa 2340 taccacgtcg agtga 2355 <210> 12 <211> 1005 <212> DNA <213> Pseudomonas putida phage <400> 12 atgagcgacc tgaccagcct gctggaaggt ctgccatccc agttcgagcg ttccgtctcc 60 ggacggacgc tcattctgga tgctgacttc cccgcgtatg aggcctcggc cacggtgaag 120 aagttgccca ccgccctgac ccgcttccag acgctggtgg aaaccgaggt gttcctgacc 180 agctccatgg atgtgcaggt gcacctgacc ccgcgtggct gcacgaagtg ccggcgatac 240 gactacccca cggccaagcc ttaccaaggc aaccgtgaag gcaaggctaa gccgccgctg 300 ctggagccgc tgcgcatggc catcccgagc cacccttggc ctgagcactg gtctacgttc 360 gcatggcgtg accgcgaggc cgacgacggc atgatgatgc acagcttcgc cctcggggca 420 aacggactgg tctgctccgg cgacaaggac ctgtgcctga ccccacatcc gtactggatc 480 atcgacgagg gccgggtgga catcatcaaa gaccggttcg gctggatcaa gcgcaaggag 540 tgggacaagg actggaagat cgtcggccac ggcacgaagt tcttctggtg ccagatgctg 600 atgggtgacc cagctgacaa cgtgcagggt atcctgcgct ggtacggaaa gaagtgcggg 660 aagcagggtg catgggagat catgcacgag atcgacaacg aggatgaggc ggccaacctt 720 gtgctgcagg cgtatgccga cgcagggcag gacttcttgg ccgaggccca gtgcttgtgg 780 ctgcgccggt cgctggacga ctgcgcattc aagtacctga gtgagctgga ccttaagccc 840 caactgcgcg agtggatggg catgctgcac gactaccacg gccaatactt ggccgctatt 900 gacgaacaga aggaattcga acgtggcgaa gatcaatcaa gcgaaggctc gggaactgaa 960 gcatcaggga accccagcgg catccgtctc ccgtgggaag actag 1005 <210> 13 <211> 378 <212> DNA <213> Pseudomonas putida phage <400> 13 atgctgcaag agcaaggtgg cgtgtgcgcc ctgtgtggca agccgatcga cctgagcatc 60 aagggtgaga tggtcattga ccacgaccac gagacaggcc ttgtccgcgg tgccctgcac 120 cgctcctgca acgcagccga gggcaaggtg gccaacgccg ctggacgctg gggtgccaag 180 agcatgtcgt acacggcgat cctgccgtgg ctgacgaacc tgctggcgta cctgcagaag 240 accccacagc cttacatcta ccccacacac aagacggacg aggagaagcg ccttgaggct 300 aacgccaagg cccgccgcgc ccgcgccacc agacaggcac gccaagtcgt gcgcaaatcc 360 gtaatcaaga aggactga 378 <210> 14 <211> 963 <212> DNA <213> Pseudomonas putida phage <400> 14 atgcgcctcc tgaaacttct gacgaaagac cagcatatcg aagtgctgac ccgccatgcc 60 tgcaacgtag aggcagcgca ggtatacagc atcgccatgg gccagccggt gacccgccag 120 aacgtggcgt actggcgccg catcttcatc gacgagggca ccaaggccaa ggctgaccga 180 ctgctggccg ccggccgagc actgcgcaaa gctgagccgg gcgacgacgt aggcgacatc 240 tcgttcgtgc cgaaggtggc ccaccgcatc ctcgtgatcg gagacctgca tgagccgtac 300 gtccatcctg atgcctacga cttcctcgaa cacctgaagg ccgagtacaa tccggacctc 360 gctgtgcaga tcggggacga gacagacggc catgccctga gcttccatga gtcagacccg 420 aacctcgaca gtgccggcgt ggagctggag aaggccaagg agggcctggc ccgtctgtac 480 gacctgttcc cgcagatgct ggtgtgccac tcgaaccacg gctcgctggt gtaccgccgc 540 gcgaagtaca gcggcatccc aatccagatg atcaagtcgt accgcgagat cctgttcccc 600 gggcacagcg cctgtggctg gcactgggcc ttcaactgga agatcgagac cccgctgggt 660 ccagtcctgt tccgtcacca gaccaccggt gatgttatgg acgccgccgc cgctgagggc 720 tgcaacctcg ttgtgggcca cgagcacggt aagttcggct cccgctgggg tgccaccagc 780 atgcgcctgt tctatggtgc atacaccggc tgcctgatcg acaaggacag catggccttc 840 gcgtacggca agacgtaccc gcgcaagcca atcctcggtg ccctcctgat tatcgagggc 900 gttcctgtta acgtgccgat gctgctcgac agcgacggcc gctgggtagg gccacagcaa 960 tga 963 <210> 15 <211> 270 <212> DNA <213> Pseudomonas putida phage <400> 15 gtgatcgccg ccctagcggt gttcaccgca gcgtacgcca ccgtaatgct gctggggctg 60 cagtccaagc tcatgcgcga caacaactgg cgcctgagct tcttcaccag ctggttcatc 120 acgttcgccc aaacgggcag cacgtacgcc atcgcccaca acgccctgcc catcgagtgg 180 ttcctgttct tcagcggcct cggcggcagc accggcattg tgtcggcaca cttcgtttac 240 gattggtacg acaagaggtt taaacgataa 270 <210> 16 <211> 2436 <212> DNA <213> Pseudomonas putida phage <400> 16 atggacctga ttcaacggca aatcaacatc gagcaagagt acaccgccca aggcattgcg 60 ctggcaatca agaagtggga gacggacatt gccgagggcc gtatcgcgga cactggtgtg 120 ggtaagcgca tggtgatacg tgcgctagcc ggtgttcgct ccgaactgga tagcatctgt 180 aatgccggta cacgcggcgt gggcggccgc tatcgcagcc tgctacgtga tatcgggctg 240 gacattgcag cggtcatcgg cctgcgcgcc ggcctcggct tagtcaccgg ccagcgtgcc 300 cgtaacggcg agccccttgt gcaggacctc cttagcgaag taggcgcaca cctagagatc 360 gagcatatgg cggtcaagct gaaggcagct gccccagcat acctaggaag ggtcttggac 420 agcctgcagg aggcccgtac gcgcagttct aaccacagac gacgtaccct acaggctacc 480 gccgataacg tctctgtggg ctctgagaac gtcacatgga gcaccgggga gcgagagggt 540 gccgctaagc tggtgctgga ggcgctggtt aatggcgggg tgctggacat gtaccacatc 600 cccaaaagcc gtggccagag ctgggtggcc ctcaaagcca gcgatgccat tgctgcgcac 660 gtagatcgca tgggcggtgc ggtgcgggca ttcatgctgt ttccgccaat gcttgtgccg 720 ccgaagccgc atacccgcga cacactgttc tctggggcgt catacctcac agagggtatg 780 tcaatcaact cggcaagtat tcgagtacgc tcccggcgtg cagaccatcg acagtggatc 840 aaggacaatg taagtgatac ggtcctaact gccgcgaacg tggccgcatc tcagccgtac 900 tgcatcgatg tggagacggc ctcgatcctg cgcgacctgt tctcccaagg tgtttacaac 960 ggcatcgcgg gcataccgtc acgtgatccc atccagccgc cggcgtatcc gctcaaggag 1020 aactgggacc gcgaggaccc tgctctacag gaggagcacg ctgcgtggaa ggtgctggct 1080 cggcaggcct acgccgacga gctggcccgg aagtcgcacg ttgtgtcgtt cttgcagacc 1140 ctgaagtatt tgaaggagta cgcaggagat accctgtatt tcccaacata cttcgactgg 1200 cgcggccgtc tgtacttccg cagccggatc aacccgcaag gcacagactt tgtgaaggct 1260 gtgatccagt ttgcgaacaa gaagccgctg ggtaaacgtg ggctgtactg gctgaaggtg 1320 catgtggcca cctgctacgg cttcgacaag aagctgccag actgccgggc ggtgtgggtg 1380 gacgagcaca tggcgcagat caaggacgct gtggcccgac acatcgactc cgacttcttc 1440 cgtgaggccg acagcccgtg gtgcttcttc gtggccgcac gggagatgct gcgcgccatc 1500 gaatccggcg accccgagtc gttcgagaca ggcgtgcccg tcgccatgga tgctacttgc 1560 agcggtatgc agcacctatc ggcagtgctc cgcgatcccg tgggtggtat gttcacgaac 1620 ctgctgttca ataacgggac cgagaaggaa gacatctacg ccggtgttgc agcaattgct 1680 atatcccgac tgcaggctga ccgggacaat cctgagcagg ccgcgtactg gctgacccac 1740 ggggtgcccc gcagcatggc aaagcgccca gtaatgacgt acgtgtacgg cggcaccctg 1800 atgtcctgcg gggattacgt gctgctggac atgcaggagc gcgggctgga ggccttgccg 1860 ggtacagca tgtcgaagct ggccgtgtac ttgggccggc atctgcggca cggtatcgag 1920 ctggcggtgc cctccgccgc gtctgctatg cggttcctgc gtgcgcttgc cggcatgatg 1980 ccccgcgatg agcccatgcg ctgggtatca ccagcgggat tccctgtgat ccagcattac 2040 gcggccgagg aggttgtgcg gtttgacctg caagggttgg gggtcatggt gagcatgacg 2100 cgctttgacg ataaccgact gaatcgctcc aagtgtgtga acggcatcgc cccgaacttc 2160 gtgcacagcc ttgacagcgg gcacttggtg cgtgtgctgg ccgcgtacaa gggcagcctt 2220 gtgcctatcc acgattcgtt tgccacgcac ccatcggatg tggatgacat gcactgggtg 2280 ctgcgcgatg agttcgtgaa gatgtacgag gagtcggacc cgatggacgc gttggtgcag 2340 ggtgtgcagc aattccgcga agaggtgatc gagcagcctg taaggggcac actagacatc 2400 tcgaaggttc gggattctgt gttcttcatg tgttag 2436 <210> 17 <211> 1515 <212> DNA <213> Pseudomonas putida phage <400> 17 atgaaggcac cagtcacata cgagagcttg ttcaccaagt accgtgacga cagcgccgta 60 ctgcggaacg agcagtatgc gctctggagc atccccacga tctactcgga ctcagatgtc 120 cgcacggacg ggaagcagca gagcgtacgc cgcgacttcc agtccgctgg cgccatgctg 180 gccaaccact tggcgagcaa gatcgcatcg ctgctgttcc cgtccactca gtcgttcttc 240 cgccttgatt cggcagtgaa ccctgacgag atggcctcgg cactggccgt ggaccagaac 300 gagctggcct ctgggctggc cacgctggag aacactgcgt accgccgcat cttcctccgc 360 tcgtcgtacc atcagctcgt gcatgccatg aagctgctga tctgcacggg gaactgcctg 420 ctgtaccgcg atagcgcgaa cacgaatatg cacgcctaca gcatgcgctc gtacgccatc 480 ctgcgggatg ggtcgggcaa ggtgctggat atcatcctga aggagcgcac cacagtcggg 540 cagctgcctg aactcctgca gagcacggcg tatgccgggc gcgaggagta cgatagcgtg 600 tgcctgtaca cccgcatcaa gcgtgagcgc cgcaaagtca ccgacgtatt cgtggtgacc 660 cagcaggcgg actcgcatgc gctggatgtg cgcgaggagt acccggaggc agtatgcccg 720 tacatccccg taacgtggaa cctcatcacc ggtgagaact acggccgtgg cctcgtggag 780 gactacgccg gcgacttcgc caagctgagc gagctgtccg aggccctggc cctgtacgag 840 attgaggcat gccgcgtgtt gcatatggct gccccgggct ctcaggccga catcgattcg 900 atggcatccg aggagatggg cgcttgggta gcgggtgacc ccaccaaggt gcaagcgtac 960 gaggctggcg actacaacaa gattgtggcc ctgaccgcgg acctgacgca aatcttccaa 1020 cgacttgcgc cggcgttcat gtacggcggg aacgtgcgag atgccgagcg ggtaaccgct 1080 gaggagatcc gtacgcaggc cgaggaagcc aaccagtcgc tgggtggtgt gtacagcgtg 1140 atcgctgata acctgcacat cccactggcc cacatcctgt gtgccgaggt gaaccctgac 1200 ttcgttgccg aggtaatcgc cggcggcctg accttgagtg tactcaccgg cgtcgccgcc 1260 ttgggtcgca gctccgatgt gaataaactc ctgcaggttg cacaggtgtt gggtgccacc 1320 ctcccagtat tcactcaggt gagccagcgg ctcgaccctg agcgcgtcat ctccaaggtg 1380 ttcgaaggct ttggcctgaa catcgaagac tattcccata ccgcagaaga gttggaagcc 1440 aaacaggccg ctaacgctgc acccgtggac actgccctga ccggtgccgc agctgacatc 1500 caaggagtcc tgtaa 1515 <210> 18 <211> 834 <212> DNA <213> Pseudomonas putida phage <400> 18 atgtctgaga accagaccca agatcaagta aacgagttcg gcagcaagat cagccccaac 60 gctgacgcga atgttgccct gaacgagaac ggccctgatc agggtactct tgctcaaatc 120 cttgcagcca tccgtggtaa ggacgttgag acgcagccag ctgccgccga caagggcaac 180 ctcggtgttg atgctgatgc cgacgagaag ccactgccaa ccgctgacga cgaggacttc 240 aagttcgaga ccggcaacaa ggcgctggat gtggctgtat cctcgttcct tcgcagcacc 300 ggtgcaagcg atgccgacat cgagcgcgca tgcaagtccg ctctggagta tggtgaccct 360 aaactgatcg accgcgcctt cattgctgag aagttcggtg accgcgcagc ggacgccgtg 420 gctctggccg aggccgtaat cgagcaggcc ggggttgaga aacaacgtac cgtccaagct 480 gtgtacgatc tcgctggctc gaaggagcag tgggacaccg cactgggtgt gtacaagcag 540 cacgcccccg ctggcctgca gaaggcgctg gggattatgt tcagctcggg agacgccgca 600 tccgtgaagg aagcagccgg tctcgtgttg gagtttgcca aaggcagtgg agttctcccc 660 gtgggtgcgg ggcaacgcca agtggctgga ggcggtaccg ctgcctctga aggcctctcg 720 gctcaagaat tcaaggccgc actgagtaag ctgaacccga acgcacgcag ctaccatcag 780 gactatgagc gcctgttgaa catgcgtcgt gtcggcaagc agctcggcat gtaa 834 <210> 19 <211> 990 <212> DNA <213> Pseudomonas putida phage <400> 19 atggctaaca ccgcctacac tggcaacctg actcgcccgc actggggtgg tccgaactcc 60 gacgctgaca tccatctgga ggtcttcgac ggtgccctcg acaccgcgtt ccagtacaac 120 gcgttcttcc gtgccaacag caccttcatc tcggtgaacg atcgttcgaa caccgcccgc 180 atcgaccgtc tgggcactgt gaccatcaag ggtcgtaagt ccggcgaggc gctcgatcct 240 actccggtca agaacgacaa gctggtaatc accgtggaca ccgtgaccta cgcacggtcc 300 ccgatcgatt accaagacga ctggaccgct ccggacttcc tgccggctat cggccagaac 360 cacggtaccg agcacgccaa gctgttcgac caagcgcaca tcatccagct gatcaagtcc 420 cgctcgtgga ttgccccggc tcacctgaag ccagccttct acgacggctt cgagaagacc 480 gcccagctgg tgaccaacga cgctgctgcc tcgtctgacg ccatcgtcgc tgcccacctg 540 agtggcatcg agaccctgat caaccgtgat ctgggtggct cgctgaacga gttcatcact 600 ctggtgaccc cacgcgtgtt ctcgctgctc ctgcagaacg agaaactggt gaacgtccag 660 tacagcgacg gtaacgcaaa ctacgcccag cgccgtgttg cgtacctgaa cggtacccgt 720 ctggtcgaga ccgctcgctt cccgaacgct gtcatcacca accaccagtt gggcgctgcc 780 ttcaacgtcg actccaccga catccaagta gagatgatcg tgtatcatcc gaagatgact 840 ctggtgaccg ttgaggccaa ggcgcacacc tcccgcgtgt gggacgatga gctgaacttt 900 gcgaacgtgc tcgactcgta cgccatgtac accatcggcc aacgccgccc agacactacc 960 ttctcggtac gtctgctgcc tggcgcgtaa 990 <210> 20 <211> 555 <212> DNA <213> Pseudomonas putida phage <400> 20 atgacacttt tagaggcggt aaacacctgt ctgtccgccc ttggcgaatc gcgggtcacc 60 agcaccacag tagtgcaccc tacggtggac ctcgtcctga gcaccatcgc catgaagcag 120 cgccagctgc tggagcgcgg gtggtggttc aacatgagcc acgtcaccat gtacccatcc 180 accacgggtg agatggagta ccccgccaac gccattgcca tcgtcggtga tgagggcgaa 240 atcttcgtgc cgcgcggcgg catgctgttc gatatggaca acaacaccca gtatttcacg 300 gagcccaaga gcatgaccgt ggcgtacaac ctcgacttcg aggaccttcc cgagtgtgct 360 gcaaacgtcg ttatgacccg cgcggcgcag gaagtgtatt cgggtgacct tggcccggac 420 gctgctgtgc agcgcctgca gggccttgag atcatgagct ttgccgagat ggagttgcag 480 cacatgcgca accgccggta cagcaccaaa caacgtcgcg gatggggtcg tctcgtccgt 540 gccctgcgcg gctag 555 <210> 21 <211> 2334 <212> DNA <213> Pseudomonas putida phage <400> 21 atggcggcta tcgacggcgc aaccaaatcg ttaatgcagg gcgtgtccca gcaagtaccc 60 cgcgaacgtc tcgacggcca ggtctccctt caggtgaaca tgctgtcgga cgccgttgaa 120 ggcatgcgcc gccggcccgg tatgcggtac ctgaccagca acatcttccc gggcggcgct 180 tggaacaaag agcaggtgtt cgccacctcc gtggacattg gggacgagcc ccaccacatc 240 ctgatcaaca ccgccaccgg cgttgtgaac gtgtacaaca gcaatatgtc gctggtggct 300 actgacgact tcttctacct gctggcgcct accgctgcca gtattcaggt agcggcatta 360 cgtgggtacc tgtacatctg caacactgag cagttcccag tcaagaacgt gactaatgcc 420 ggcaagcaga accctgacaa gacgggtttc ttctttgtga agacggggca gtacagcaag 480 atttacaacg tcactctgaa cgtgaacggg gtgcagtacg cctcatcgta cactacaccg 540 aacggatccg cccccggcga tgccgccctg agcacgcctg agtacgtagc tggcaagctg 600 gtggacggta ttgtggcaca cgccattccg ggcctctcct ttgccatcgc gggctcgtac 660 gtgtaccttg tcaccacggg tgctggggcc atcactgtga cctcagattc cggcacaaac 720 ttcatcagcg cctcgaacca gtcccatgtc accctgacta ctgagctccc tgcccggatg 780 cccgcagctg ccaacggcat gctggtaagc gtgggcgcca acccatcgct atccgtgtgg 840 taccgctttg acttcgtgaa caacgcgtgg gttgagtcgg gggcttggaa cagcgccgac 900 ttcctgacga atatgcccat ccggttgaag ctggataact cctacgtgat ggaccagcca 960 aactatgagg ggcgtttgtc gggtaacgac aacacgaacg aggaccccgc cttccttacg 1020 gacggcgtga ccggtctgag cgtattccaa ggccgtctgg taatcctaag cggggcaacg 1080 gtgtgtatga gcgctgcggg caaacccctg cgctggtacc gtagcaccac tacggaactc 1140 ctcgttgccg atcccatcag catcttctca ggctcggcta ccactgccaa cttcacacac 1200 gccatccagt ttaacaagga cttgttactg tttgccaaga cctgccaagc ggtagtgccc 1260 tcgggtaact ctgtagtggc gcccagtacg gcccagatcg tcatcaccag tgcgtacaac 1320 acaactagca aggtgctgcc tatcgtggca gggcggtccc taatgtactt cgcaccccgc 1380 agcgagaagt tcgccagcgt cctagagatg gtgccgagta acaccacgga ctcgcaatac 1440 accaccaacg acgtaactgc acacatccca cagtacatgc cggggaccat tcggcaggct 1500 acggctagca ccaccagcaa cagccttgtg cttgtcgctg acggggatgc ccgcaccctg 1560 tttgtgcacg agtacttgtg gggtggggat gagaaactgc agtctgcatg gcataactgg 1620 accgcaccgt atgacatcgt gtgtacttgg tttgtccgcg acacaatcta cgtcggtatg 1680 ttagtagacg gcgacctcgc aattgtcacg atcgactcac aggcaggcga cacagccaac 1740 ggcctgtacc gcccgttctc ggacatgttc atcggcactg cagtcgtcgg cggcaccttt 1800 acggttccca cggcgctgcg cgctgcatac acagccggag ccgagttgca gttgacctac 1860 accaccggcg cagttgctgg cgaggcagta ggcttcagca tcaacacagc cacttgggtt 1920 ggtacagcgg tgcgtaacgt ccctgacggc acgtacttcc taggcctcaa gtaccgcagc 1980 acgctgtcac ctacaccacc gctactgcgc gatcagaacg gtgtggtgat tggaaccgca 2040 ggcacatccc tgatccggta cgaggcctcc ctgaagaaca cgggtgattt ccatgtggtt 2100 gtgcaacggc acgacgagac tacctcggaa ggggtatact ccggccttac atacagctcg 2160 caggacttga tccccggtgg gcccctgtac gccaagagta gccggtgcat catcccagtg 2220 cgtttgaacc ccgacgcctc tagcgtgatc atgtacgcag aagacgatca cgacctcggc 2280 gtgcttaccc ttgagcacgt tatgcagtac caccctcgac gacggagaat gtaa 2334

Claims

A bacteriophage [KCTC18380P] comprising a dielectric having a specific cytotoxic activity against carbapenem-resistant Pseudomonas putida and represented by at least one of SEQ ID NOS: 2 to 21.

The method according to claim 1,
Wherein the bacteriophage comprises a dielectric represented by at least one of SEQ ID NOS: 2, 7, and 15 [KCTC18380P].

The method according to claim 1,
The bacteriophage is a bacteriophage consisting of the genome of SEQ ID NO: 1 [KCTC18380P].

delete

An antibiotic comprising the bacteriophage [KCTC18380P] according to any one of claims 1 to 3 as an active ingredient.

A disinfectant comprising the bacteriophage [KCTC18380P] according to any one of claims 1 to 3 as an active ingredient.

delete