KR20230171302A - Novel bacteriophage specific for fire blight and black shoot blight and use thereof - Google Patents

Abstract

The present invention relates to a bacteriophage with accession number KACC 97039P, which shows specific lytic activity against Erwinia amylovora and Erwinia pyrifoliae, causing fruit burn disease and fruit tree branch black. It has a control effect on blight, and can be used as a microbial pesticide to control fruit tree burn and black blight on fruit trees. It can be used instead of antibiotics, so it prevents the emergence of antibiotic-resistant strains and does not affect the environment, making it safe and effective. Control of fruit tree burn disease and fruit tree branch black blight can be expected.

Description

Novel bacteriophage specific for fire blight and black shoot blight and use thereof}

The present specification relates to a bacteriophage having specific and strong lytic activity against fruit burn blight and eggplant black blight, a pesticide composition containing the bacteriophage, and a method for controlling burn blight and eggplant black blight using the same.

Fire blight is one of the representative bacterial plant quarantine diseases, and Erwinia amylovora is known to be the causative agent. Fruit burn disease causes the flowers, branches, and fruits of pear or apple trees to turn black and die, as if they were burned. Erwinia amylobora is transmitted by insects or wind and rain and invades the host through flowers, nectar glands, stomata, epidermis, wounds, etc., so it is highly contagious and can infect over 70 species of plants, including pear trees, apple trees, and apricot trees. Due to this characteristic, damage from burn disease is occurring worldwide.

Because orchard burn disease spreads quickly, the entire orchard must be closed even if only one tree shows symptoms. For this reason, fruit burn disease not only ruins the year's harvest, but can also cause long-term damage to farms.

In addition, black blight of fruit trees is one of the indigenous bacterial plant quarantine diseases, and Erwinia pyrifoliae is known to be the causative agent. Black blight on fruit trees causes the flowers, branches, and fruits of pear or apple trees to turn black and die.

Black blight on fruit trees spreads relatively slowly, but even if only one tree shows symptoms, the entire orchard must be closed, and fruit trees within a 100m radius must be disposed of by uprooting and burning or burying them in the ground. For this reason, black blight on fruit trees not only ruins the year's harvest, but can also cause long-term damage to farms.

As a response to the above disease, a method of controlling the infection and spread of fire blight is achieved by reducing soil moisture and maintaining the balance of fertilizer nutrients, and by removing infected fruit trees or blackened branches in winter when the pathogen has a dormant period. There are known methods to treat the disease, but the occurrence of the underlying disease cannot be eliminated.

The present inventors studied phage therapy, a control (treatment) method using bacteriophages, as a control method for Erwinia amylovora and Erwinia pyrifolia, and found that a new bacteriophage isolated from water in a pear orchard was used against Erwinia amylovora. It was found that it showed strong lytic activity.

One aspect seeks to provide a bacteriophage having specific and strong lytic activity against one or more bacteria selected from the group consisting of Erwinia amylovora and Erwinia pyrifoliae.

Another aspect is to provide a pesticide composition containing the bacteriophage, specifically, a pesticide composition for controlling fruit burn disease or eggplant black blight.

Another aspect seeks to provide a disinfectant containing the bacteriophage as an active ingredient.

Another aspect seeks to provide a detergent containing the bacteriophage as an active ingredient.

Another aspect is to provide a method for controlling fruit burn disease or branch black blight using the bacteriophage.

The present invention provides a bacteriophage, accession number KACC 97039P, which has specific lytic activity against Erwinia amylovora.

The present inventors selected a new bacteriophage with excellent lytic effect and host specificity against Erwinia amylobora, named it Erwinia phage Fifi106 (hereinafter referred to as Fifi106), and deposited it with the National Institute of Agricultural Sciences on August 5, 2022. Therefore, KACC 97039P was granted.

The present inventors isolated bacteriophage Fifi106 from the water of a pear orchard in Seoun-myeon, Anseong, Gyeonggi-do, and confirmed that it formed plaques and lysed Erwinia amylovora in an experiment conducted using Erwinia amylovora strain YKB14808 as a host (Figure One).

Referring to Figure 2, it can be seen that the growth of host cells can be inhibited even when the MOI (multiplicity of infection) level of bacteriophage Fifi106 is low (the control in Figure 2 is when bacteriophage Fifi106 is not introduced).

The bacteriophage Fifi106 was purified by CsCl density gradient ultra-high-speed centrifugation, and the shape of the phage was confirmed using an electron microscope, as shown in Figure 3. It was found to belong to the Myoviridae family, which consists of a head of approximately 74 nm and a tail of 114 nm.

The bacteriophage Fifi106 of the present invention is effective against all strains within the Erwinia amylovora and Erwinia pyrifoliae species. It does not show lytic activity against Dickeya zeae, Enterobacter cloacae, Escherichia coli, and Pantoea agglomerans.

In addition, since the bacteriophage of the present invention does not infect other hosts other than bacteria such as plants, it has the advantage of solving the problems of antibiotic-resistant bacteria due to misuse of antibiotics, the problem of antibiotic residues in food, and the problem of a wide host range.

Therefore, the bacteriophage of the present invention can be used in various fields such as prevention or treatment of infectious diseases caused by Erwinia amylobora and Erwinia pyrifolia bacteria, antibiotic compositions, feed additive compositions, feed, disinfectants, or detergents. there is.

Bacteriophage Fifi106 of the present invention has the base sequence of Sequence Listing 1. Specifically, bacteriophage Fifi106 has a base sequence of 84,405 bp.

Bacteriophage Fifi106 may be active at 15°C to 70°C, 20°C to 70°C, 25°C to 70°C, 30°C to 70°C, 35°C to 65°C or 40°C to 60°C. Specifically, it showed lytic activity against Erwinia amylobora even when treated at 50°C for 1 hour.

Bacteriophage Fifi106 may be active at pH 1 to 15, pH 2 to 14, pH 3 to 13, pH 3 to 12, pH 4 to 12, and pH 4 to 11. Even when exposed to various pH, it shows lytic activity against Erwinia amylobora and Erwinia pyrifolia.

According to another embodiment, the present invention provides a pesticide composition containing the bacteriophage as an active ingredient.

The present invention can provide a composition that has excellent growth inhibition and killing effects on all strains within the species of Erwinia amylovora and Erwinia pyrifoliae, and this composition contains the bacteriophage Fifi106. Included as an active ingredient.

The bacteriophage of the pesticide composition is used for the prevention or treatment of fire blight and black blight of fruit trees.

The pesticide composition according to the present invention described above can be used on fruits that may suffer from fruit burn disease, such as apples, pears, etc., but is not limited thereto. In particular, the present invention applies to plants of the Rosaceae family such as apples or pears. It is effective in controlling fruit tree burn disease and fruit tree branch black blight.

Meanwhile, any one selected from the pesticide composition of the present invention, the composition for controlling fruit burn disease, and the composition for inhibiting or killing the growth of Erwinia amylovora and Erwinia pyrifoliae is a kind of microbial pesticide. It can be manufactured in various forms such as liquid, powder, etc.

In addition, if necessary, various known excipients used in microbial pesticides may be added. Since the excipients are not limited to those used in microbial pesticides and can be used, a description of the excipients will be omitted. For example, the pesticide composition according to the present invention may use surfactants or extenders that can be used as biochemical pesticides as excipients for the purpose of forming a stable formulation suitable for use in actual packaging. In this case, the pesticide composition may be prepared in one or more forms selected from the group consisting of wetting agent, granular wetting agent, liquid wetting agent, liquid agent, water solution, water-soluble granule, and encapsulation agent. Examples of the surfactant include alkyl (C8 to C12) Aryl sulfonate, dialkyl (C3~C6) aryl sulfonate, dialkyl (C8~C12) sulfosuccinate, lignin sulfonate, naphthalene sulfonate condensate, naphthalene sulfonate formalin condensate, alkyl (C8~C12) Naphthalene sulfonate formalin condensate, sodium or calcium salts of sulfonate compounds such as polyoxyethylene alkyl (C8-C12) phenyl sulfonate, alkyl (C8-C12) sulfate, alkyl (C8-C12) aryl sulfate, polyoxy Sodium or calcium salts of sulfate compounds such as ethylene alkyl (C8-C12) sulfate, polyoxyethylene alkyl (C8-C12) phenyl sulfate, sodium or calcium salts of succinate compounds such as polyoxyalkylene succinate, sodium Anionic surfactants such as benzoate and alkyl carboxylate, polyoxyethylene alkyl (C8~C12) ether, polyoxyethylene alkyl (C8~C12) phenyl ether, polyoxyethylene alkyl (C8~C12) phenyl polymer, ethylene Nonionic surfactants such as oxide propylene oxide copolymer, polycarboxylates, one or two or more selected from the group consisting of Triton 100 and Tween 80 may be used in combination, and other surfactants other than these may also be used. It can be easily understood by those with ordinary knowledge in the relevant technical field.

Extenders include bentonite, talc, clay, kaolin, calcium carbonate, silica sand, pumice, diatomaceous earth, acid clay, zeolite, perlite, white carbon, ammonium sulfate, urea, glucose, dextrin, soybean flour, rice, and wheat. , red clay, glucose, starch, water, etc. can be used alone or in a mixture of two or more, and it can be easily understood by those skilled in the art that other extenders other than these can also be used. will be.

The present invention provides an antibiotic composition containing the bacteriophage as an active ingredient.

The present invention provides a composition for preventing or treating diseases caused by Erwinia amylovora and Erwinia pyrifoliae, comprising the bacteriophage as an active ingredient.

The antibiotic composition, preventive or therapeutic composition containing the bacteriophage as an active ingredient is safe as it does not induce drug resistance or resistance compared to existing antibacterial substances or antibiotics, and is effective against Erwinia amylobora and Erwinia pyrifolia. Has high specificity.

The present invention provides a disinfectant containing the bacteriophage as an active ingredient.

The present invention provides a detergent containing the bacteriophage as an active ingredient.

The disinfectant or cleaner containing the bacteriophage as an active ingredient has excellent disinfection or cleaning effect and has high specificity against Erwinia amylobora and Erwinia pyrifolia.

The present invention provides a method for controlling fruit tree burn disease or fruit tree branch black blight using the bacteriophage Fifi106.

The overhydration disease is caused by Erwinia amylovora, and Erwinia amylovora can be killed by treating the bacteria with a pesticide composition containing the bacteriophage as an active ingredient.

The black blight of fruit trees is caused by Erwinia pyrifoliae, and Erwinia pyrifolia can be killed by treating bacteria with a pesticide composition containing the bacteriophage as an active ingredient.

Phage therapy, a control (treatment) method using bacteriophages according to the present invention, prevents the emergence of antibiotic-resistant strains and does not affect the environment, so safe and effective control of fruit tree burn disease and fruit tree branch black blight can be expected.

The present invention provides the use of the bacteriophage for the prevention or treatment of one or more diseases selected from the group consisting of fire blight and fruit tree blight.

The present invention provides the use of the bacteriophage in the production of a pesticide composition for preventing or treating one or more diseases selected from the group consisting of fire blight and black blight of fruit trees.

Specific details regarding the bacteriophage, fruit tree burn disease, fruit tree branch black blight, prevention, treatment, pesticide composition, etc. are the same as above or may be applied mutatis mutandis.

It should be understood that the terms used in this specification are only for describing specific embodiments and are not intended to limit the scope of the present invention, which is limited only by the scope of the appended claims. All technical and scientific terms used in this specification have the same meaning as generally understood by those skilled in the art, unless otherwise specified.

In this specification, when a part “includes” a certain component, this means that it may further include other components rather than excluding other components, unless specifically stated to the contrary.

Meanwhile, various implementations and embodiments of the present invention may be combined with any other embodiments unless clearly indicated to the contrary. Any feature indicated as being particularly preferred or advantageous may be combined with any other feature or feature indicated as being preferred or advantageous.

As used herein, “bacteriophage” is a virus that infects a specific target bacteria. The head of a bacteriophage, enclosed in a protein capsid, consists of a nucleic acid genome, and some bacteriophages have a tail composed of lipids or proteins. Most bacteriophages contain double-stranded DNA as their genetic material.

“Pesticides” in this specification refer to drugs used for the protection and growth of crops, including chemical pesticides and biological pesticides used to control pests, pests, and weeds that occur during cultivation or storage of crops, and physiological functions of crops. It is a general term for growth regulators used to promote or suppress, and adjuvants that enhance medicinal efficacy.

In this specification, “control” means preventing and exterminating various diseases and pests that damage crops.

As used herein, “antibiotic composition” refers to a preparation that can be provided to animals and plants in pharmaceutical form to kill bacteria, and is a general term for preservatives, disinfectants, antibiotics, and antibacterial agents.

As used herein, “antibacterial agent” refers to a substance that kills or inhibits the growth of microorganisms, and includes preservatives, disinfectants, and antibiotics.

In this specification, “disinfectant” can be useful as a disinfectant for hospitals and public health to prevent hospital infections, and can be used as a general household disinfectant, a disinfectant for food and cooking places and facilities, buildings such as orchards, livestock, drinking water, litter, and litter. It can be used for disinfection of various growing supplies such as transport vehicles and dishes.

As used herein, “cleaning agent” means a chemical used for the purpose of cleaning.

The bacteriophage newly isolated in the present invention, accession number KACC 97039P, has specific lytic activity (killing ability) against Erwinia amylovora and Erwinia pyrifoliae. It has no lytic activity against other Erwinia species, so it has a very high specificity against fruit tree blight and black blight on fruit trees, and is strain-dependent because it is effective against all strains within the same Erwinia amylobora and Erwinia pyrifolia species. It's not. In addition, it has high stability against temperature or pH changes, making it an environmentally friendly control agent for fruit tree burn disease and black blight on fruit trees.

Figure 1 shows the formation of plaques by the bacteriophage of the present invention in an experiment conducted using Erwinia amylobora as a host.
Figure 2 confirms the lytic activity of the bacteriophage of the present invention against Erwinia amylobora.
Figure 3 shows the bacteriophage of the present invention confirmed by transmission electron microscopy.
Figure 4 relates to the lytic activity of the bacteriophage of the present invention according to temperature.
Figure 5 relates to the lytic activity of the bacteriophage of the present invention according to pH.
Figure 6 confirms the effect of controlling fruit burn disease in yugwa.
Figure 7 is a diagram showing the genome map of the bacteriophage of the present invention.
Figure 8 is a diagram showing the phylogenetic tree of the bacteriophage of the present invention.

Hereinafter, the present invention will be described in more detail through examples and experimental examples. However, these examples are for illustrative purposes only and the scope of the present invention is not limited to these examples and experimental examples.

Examples and Experimental Examples

Experimental Example 1. Bacteriophage isolation

1.1. Pretreatment of environmental samples for bacteriophage isolation

이다. 배양된 시료는 10,000×g에서 5분간 원심분리하여 상층액만을 새로운 tube에 옮겨 담는다. 이렇게 얻어진 시료는 세균을 완전히 제거하기 위해 0.2 μm pore size filter로 여과하였다. To isolate bacteriophages, we collected soil and water from an orchard where fruit tree burn disease occurred (pear orchard in Seoun-myeon, Anseong, Gyeonggi-do) and nearby areas. Place 10 g for soil samples and 10 ml for water samples into a 50 ml conical tube and add 10 ml of TSB (tryptic soy broth). Here, 1 ml of a mixed culture containing 94 strains of fruit burn pathogen ( Erwinia amylovora ) cultured with shaking for 24 hours in equal proportions was added and cultured with shaking for 24 hours. Incubation temperature is 27

am. The cultured sample is centrifuged at 10,000×g for 5 minutes, and only the supernatant is transferred to a new tube. The sample obtained in this way was filtered through a 0.2 μm pore size filter to completely remove bacteria.

1.2. Dotting assay

로 준비하였다. 배지 3ml에 밤새 진탕 배양한 과수 화상병균 1종을 15 μl 넣고 기포가 생기지 않게 섞은 후 TSA(tryptic soy agar)에 얇게 부어 10분간 굳힌다. 균주가 균일하게 포함된 배지 위에 여과한 환경 시료를 10μl씩 떨어뜨인 후 27

에서 정치 배양한다. 여러 개의 환경 시료를 하나의 배지에 함께 떨어뜨릴 경우 시료가 서로 섞이지 않도록 충분한 간격을 유지하여야 한다. 24시간 배양 후 시료 부분에 세균이 자라지 못하고 투명하거나 플라크가 확인되면 양성으로 판정하였다. A dotting assay was performed to confirm the presence of bacteriophages in various environmental samples that had been pretreated to induce the proliferation of bacteriophages. Add 0.4% soft agar to TSB and sterilize the medium for 40 to 50 minutes.

It was prepared with Add 15 μl of one type of fruit burn pathogen cultured overnight with shaking to 3 ml of medium, mix to prevent bubbles from forming, then pour thinly on TSA (tryptic soy agar) and harden for 10 minutes. After dropping 10 μl of the filtered environmental sample onto the medium containing the strains uniformly, 27

Cultivate politics in. When dropping multiple environmental samples together on one medium, sufficient spacing must be maintained to prevent the samples from mixing together. After 24 hours of incubation, if bacteria did not grow in the sample area and were transparent or plaques were identified, the test was judged positive.

1.3. Plaque assay (overlay assay)

로 준비한다. 배지 3 ml에 밤새 진탕 배양한 과수 화상병균 15 μl와 박테리오파지가 확인된 양성 시료를 10μl 넣고 기포가 생기지 않게 섞은 후 TSA에 얇게 부어 10분간 굳힌 후 27

에서 24시간 동안 정치 배양한다. 이때 양성 시료는 단일 플라크를 얻기 위해 십진희석하여 농도별로 수행해야 한다. 24시간 배양 후 크기나 모양이 다른 플라크(plaque)를 선별하여 1 ml의 saline-magnesium(SM) buffer에 넣어 냉장 보관하였다. 각 플라크에서 추출한 박테리오파지는 단일 박테리오파지 분리를 위해 동일한 과정을 5번 이상 반복하였다. 이때 형성된 플라크(plaque)를 도 1에 나타내었다.An overlay assay was performed to isolate pure bacteriophages. Add 0.4% soft agar to TSB and sterilize the medium for 40 to 50 minutes.

Prepare with Add 15 μl of fruit burn pathogens cultured overnight with shaking and 10 μl of positive sample with confirmed bacteriophage to 3 ml of medium, mix to prevent bubbles from forming, then pour thinly into TSA and harden for 10 minutes.

Incubate for 24 hours. At this time, positive samples must be decimal diluted to obtain a single plaque and performed by concentration. After 24 hours of incubation, plaques of different sizes and shapes were selected, placed in 1 ml of saline-magnesium (SM) buffer, and stored in the refrigerator. The same process was repeated for the bacteriophages extracted from each plaque more than 5 times to isolate single bacteriophages. The plaque formed at this time is shown in Figure 1.

Experimental Example 2. Confirmation of lytic activity

, 120 rpm으로 진탕 배양하며 1시간 단위로 세균의 OD값을 측정한 것을 도 2에 나타내었다.Fruit tree burn pathogen ( Erwinia amylovora YKB14808) is prepared by shaking culture in TSB medium the day before and then re-cultured 24 hours later. Afterwards, when the concentration of bacteria reaches OD _600nm = 0.5 (10 ⁸ CFU/ml), the inoculation concentration is adjusted to 10 ⁵ CFU/ml by decimal dilution in TSB medium. After dispensing 180 μl of bacterial culture into a 96-well plate, 20 μl of phage is inoculated at a multiplicity of infection (MOI) of 0.001, 0.1, 10, and 1000. 27 in a microplate reader for the next 24 hours.

, the OD value of the bacteria was measured every hour while shaking at 120 rpm, as shown in Figure 2.

Experimental Example 3. Morphological analysis

에서 24시간 배양한다. 24시간 배양한 시료를 9,000g에서 20분간 원심분리한 후 상층액을 제거하고 침전물을 SM buffer로 현탁하여 준비한다. 초고속원심분리용 튜브에 CsCl 밀도층(1.3 g/ml, 1.45 g/ml, 1.5 g/ml, 1.7 g/ml)을 역순으로 3 ml씩 쌓은 후 그 위에 농축된 파지 시료를 쌓고 785,500×g에서 2시간 동안 원심분리하여 정제된 파지를 얻었다. 정제된 파지를 SM buffer로 투석한 후 0.22 μm pore size filter로 여과하였다. 여과된 파지 시료를 2% uranyl acetate 용액으로 염색한 후 투과전자현미경을 통해 형태를 확인하여 도 3에 나타내었다. To proliferate bacteriophages, the burn pathogen ( Erwinia amylovora YKB14808) is cultured in 3 ml of TSB with shaking for 24 hours. 2 ml of burn pathogens cultured with shaking for 24 hours are re-cultured in 200 ml of TSB, and when OD ₆₀₀ = 0.5, 1 ml of phage with a concentration of 10 ⁸ PFU/ml is treated and cultured with shaking for 24 hours. To remove impurities from the sample cultured with shaking for 24 hours, it was centrifuged at 10,000 × g for 10 minutes and the supernatant was filtered through a 0.22 μm pore size filter. After treating the bacteriophage sample with 5g of Ployethylene glycol 8000 (PEG 8000) and 10ml of 5 M NaCl,

Incubate for 24 hours. Samples cultured for 24 hours are centrifuged at 9,000g for 20 minutes, the supernatant is removed, and the precipitate is suspended in SM buffer. In an ultra-high-speed centrifugation tube, 3 ml of CsCl density layer (1.3 g/ml, 1.45 g/ml, 1.5 g/ml, 1.7 g/ml) was stacked in reverse order, then the concentrated phage sample was stacked on top of it and centrifuged at 785,500 × g. Purified phage was obtained by centrifugation for 2 hours. The purified phage was dialyzed against SM buffer and then filtered through a 0.22 μm pore size filter. The filtered phage sample was stained with a 2% uranyl acetate solution and its morphology was confirmed through a transmission electron microscope, as shown in Figure 3.

Experimental Example 4. Host range analysis

Host range analysis of the isolated bacteriophages included 94 strains of Erwinia amylovora , 25 strains of Erwinia pyrifoliae , Dickeya zeae, Enterobacter cloacce, Escherichia coli, Pantoea agglomerans, Pectobacterium carotovorum, Pseudomonas fluoresceus, Xanthomonas campestris, Bacillus thuringieusis, Clavibacter michiganensis, and Enterococcus faec. alis was used . AgriPhage, a bacteriophage preparation for controlling burn pathogens, was used as a positive control, and SM buffer was used as a negative control. The dotting assay method was used to analyze the host range, and if plaques were identified after 24 hours of incubation, the test was judged positive. Specifically, cases where a transparent ring is formed are indicated as +, and cases where a transparent ring is not generated are indicated as -, and are listed in Table 1 below. All experiments were repeated 3 to 9 times, and the average value of the stage scores of the observed plaques was recorded.

No. species strain Lytic activity of Erwinia phage Fifi106 One Erwinia amylovora YKB 14715 + 2 Erwinia amylovora YKB 14740 + 3 Erwinia amylovora YKB 14742 + 4 Erwinia amylovora YKB 14748 + 5 Erwinia amylovora YKB 14750 + 6 Erwinia amylovora YKB 14754 + 7 Erwinia amylovora YKB 14756 + 8 Erwinia amylovora YKB 14758 + 9 Erwinia amylovora YKB 14768 + 10 Erwinia amylovora YKB 14770 + 11 Erwinia amylovora YKB 14776 + 12 Erwinia amylovora YKB 14778 + 13 Erwinia amylovora YKB 14787 + 14 Erwinia amylovora YKB 14806 + 15 Erwinia amylovora YKB 14808 + 16 Erwinia amylovora YKB 14814 + 17 Erwinia amylovora YKB 14818 + 18 Erwinia amylovora YKB 14820 + 19 Erwinia amylovora YKB 14822 + 20 Erwinia amylovora TS3128 + 21 Erwinia amylovora RA0023 + 22 Erwinia amylovora RA0024 + 23 Erwinia amylovora RA0025 + 24 Erwinia amylovora RA0026 + 25 Erwinia amylovora RA0027 + 26 Erwinia amylovora RA0028 + 27 Erwinia amylovora RA0029 + 28 Erwinia amylovora RA0030 + 29 Erwinia amylovora RA0031 + 30 Erwinia amylovora RA0032 + 31 Erwinia amylovora RA0033 + 32 Erwinia amylovora RA0034 + 33 Erwinia amylovora RA0035 + 34 Erwinia amylovora RA0036 + 35 Erwinia amylovora RA0037 + 36 Erwinia amylovora RA0038 + 37 Erwinia amylovora RA0039 + 38 Erwinia amylovora RA0040 + 39 Erwinia amylovora RA0041 + 40 Erwinia amylovora RA0042 + 41 Erwinia amylovora RA0043 + 42 Erwinia amylovora RA0044 + 43 Erwinia amylovora RA0045 + 44 Erwinia amylovora RA0046 + 45 Erwinia amylovora RA0047 + 46 Erwinia amylovora RA0048 + 47 Erwinia amylovora RA0049 + 48 Erwinia amylovora RA0050 + 49 Erwinia amylovora RA0051 + 50 Erwinia amylovora RA0052 + 51 Erwinia amylovora RA0053 + 52 Erwinia amylovora RA0054 + 53 Erwinia amylovora RA0055 + 54 Erwinia amylovora RA0056 + 55 Erwinia amylovora RA0057 + 56 Erwinia amylovora RA0058 + 57 Erwinia amylovora RA0059 + 58 Erwinia amylovora RA0060 + 59 Erwinia amylovora RA0061 + 60 Erwinia amylovora RA0062 + 61 Erwinia amylovora RA0063 + 62 Erwinia amylovora RA0064 + 63 Erwinia amylovora RA0065 + 64 Erwinia amylovora RA0066 + 65 Erwinia amylovora RA0067 + 66 Erwinia amylovora RA0068 + 67 Erwinia amylovora RA0069 + 68 Erwinia amylovora RA0070 + 69 Erwinia amylovora RA0071 + 70 Erwinia amylovora RA0072 + 71 Erwinia amylovora RA0073 + 72 Erwinia amylovora RA0074 + 73 Erwinia amylovora RA0075 + 74 Erwinia amylovora RA0076 + 75 Erwinia amylovora RA0077 + 76 Erwinia amylovora RA0078 + 77 Erwinia amylovora RA0079 + 78 Erwinia amylovora RA0080 + 79 Erwinia amylovora RA0081 + 80 Erwinia amylovora RA0082 + 81 Erwinia amylovora RA0083 + 82 Erwinia amylovora RA0084 + 83 Erwinia amylovora RA0085 + 84 Erwinia amylovora RA0086 + 85 Erwinia amylovora RA0087 + 86 Erwinia amylovora RA0088 + 87 Erwinia amylovora RA0089 + 88 Erwinia amylovora RA0090 + 89 Erwinia amylovora RA0091 + 90 Erwinia amylovora RA0092 + 91 Erwinia amylovora RA0093 + 92 Erwinia amylovora RA0094 + 93 Erwinia amylovora RA0095 + 94 Erwinia amylovora RA0096 + 95 Erwinia pyrifoliae Ep12327 + 96 Erwinia pyrifoliae RP0098 + 97 Erwinia pyrifoliae RP0099 + 98 Erwinia pyrifoliae RP0100 + 99 Erwinia pyrifoliae RP0101 + 100 Erwinia pyrifoliae RP0102 + 101 Erwinia pyrifoliae RP0103 + 102 Erwinia pyrifoliae RP0104 + 103 Erwinia pyrifoliae RP0105 + 104 Erwinia pyrifoliae RP0106 + 105 Erwinia pyrifoliae RP0107 + 106 Erwinia pyrifoliae RP0108 + 107 Erwinia pyrifoliae RP0109 + 108 Erwinia pyrifoliae RP0110 + 109 Erwinia pyrifoliae RP0111 + 110 Erwinia pyrifoliae RP0112 + 111 Erwinia pyrifoliae RP0113 + 112 Erwinia pyrifoliae RP0114 + 113 Erwinia pyrifoliae RP0115 + 114 Erwinia pyrifoliae RP0116 + 115 Erwinia pyrifoliae RP0117 + 116 Erwinia pyrifoliae RP0118 + 117 Erwinia pyrifoliae RP0119 + 118 Erwinia pyrifoliae RP0120 + 119 Erwinia pyrifoliae RP0121 + 120 Dickeya zeae - 121 Enterobacter cloacce - 123 Escherichia coli - 124 Pantoea agglomerans - 125 Pectobacterium carotovorum - 126 Pseudomonas fluoresceus - 127 Xanthomonas campestris - 128 Bacillus thuringieusis - 129 Clavibacter michiganensis - 130 Enterococcus faecalis -

Looking at Table 1, bacteriophage Fifi106 has high specificity for Erwinia amylovora and Erwinia pyrifoliae, and is effective against all strains within the same species, so strain- You can see that it is not dependent.

Experimental Example 5. Temperature and pH stability

, 40

, 50

, 60

에서 1시간 동안 처리하여 dotting assay를 통해 활성 농도의 변화를 확인하여 도 4에 나타내었다. The stability of bacteriophages was analyzed to determine whether their activity was maintained at various temperatures and pH. The concentration of each phage was 10 ⁵ PFU/ml. Dispense 200 μl of each phage into a 1.5 ml tube and leave for 30 minutes.

, 40

, 50

, 60

After treatment for 1 hour, changes in activity concentration were confirmed through dotting assay and are shown in Figure 4.

The pH was adjusted to pH 4 to 11 using HCl and NaOH in SM buffer. After treating 10 μl of phage with a concentration of 10 ⁷ PFU/ml in 990 μl of SM buffer prepared for each pH for 1 hour, the change in activity concentration was confirmed through a dotting assay and is shown in Figure 5.

It can be seen that the bacteriophage Fifi106 of the present invention has high stability even under temperature or pH changes and has excellent effectiveness as an eco-friendly control agent for fruit tree burn disease and fruit tree branch black blight.

Experimental Example 6. Confirmation of the effect of suppressing the occurrence of fruit tree burn disease

에서 약 2주간 2일 간격으로 병 발생 정도를 확인한 것을 도 6에 나타내었다. 도 6을 보면, 박테리오파지 Fifi106이 접종된 유과는 과수 화상병이 방제됨을 알 수 있다.Prepare the fruit tree burn pathogen ( Erwinia amylovora YKB14808) by shaking and culturing it in TSB medium the day before. Re-cultivate the bacterial culture in TSB medium and when it reaches OD _600nm = 0.5 (10 ⁸ CFU/ml), precipitate the burn pathogens by centrifugation at 1,800 prepare. Immature fruits used in experiments are surface sterilized with 70% ethanol. Using a sterilized 1 mL tip, make two holes on the surface of the immature fruit. Inoculate 10 μl of 10 ¹⁰ PFU/ml phage solution into the hole made on the surface of the immature fruit without flowing. Afterwards, before the phage solution completely dries, 10 μl of bacterial suspension of 10 ⁷ CFU/ml is inoculated and the relative humidity is maintained above 90%. 27

The degree of disease occurrence was confirmed at two-day intervals for about two weeks, as shown in Figure 6. Looking at Figure 6, it can be seen that fruit tree burn disease is controlled in fruit trees inoculated with bacteriophage Fifi106.

Experimental Example 7. Base sequence analysis

Bacteriophage particles were treated with protease to remove the outer shell, and genomic DNA (gDNA) of each phage was extracted using Norgen's phage DNA isolation kit. A library was created from 100 to 200 ng of gDNA using the TruSeq Nano DNA kit, and then next generation sequencing (NGS) was performed based on the Illumina HiSeqXTen platform. The entire reads obtained were assembled to obtain a single contig. The function of each open reading frame predicted by sequence analysis was analyzed using Basic Local Alignment Search Tool (BLAST) and HHpred. The genome of Erwinia phage Fifi106 has a size of 84,405 bp, and 114 open reading frames (ORFs) and 26 tRNAs were predicted. To determine the genetic function of the sequence, ORFs were analyzed using HHpred and NCBI BLAST programs, and a genetic map was created using BRIG (see Table 2 and Figure 7).

Functional group Locus tag Predicted function Lysis 106_00020 Putative endonuclease 106_00028 Putative endonuclease 106_00107 Putative endolysin 106_00129 Putative spanin 106_00136 Putative transglycosylase Structure 106_00052 Putative base plate protein 106_00053 Putative base plate protein 106_00054 Putative baseplate wedge protein 106_00055 Putative baseplate assembly protein 106_00056 Putative baseplate hub protein 106_00059 Putative tape measure protein 106_00060 Putative tail assembly chaperone 106_00061 Putative structural protein 106_00062 Putative tail sheath protein 106_00064 Putative capsid completion protein 106_00065 Putative capsid assembly protein 106_00067 Putative major capsid protein 106_00068 Putative capsid decoration protein 106_00069 Putative head-tail preconnector protein 106_00072 Putative portal protein 106_00073 Putative terminase, large subunit Nucelotide metabolism 106_00004 Putative sRNA-binding protein 106_00005 Putative nicotinate phosphoribosyltransferase 106_00013 Putative glutaredoxin 106_00014 Putative ribonucleoside-diphosphate reductase, beta subunit 106_00015 Putative ribonucleoside-diphosphate reductase, alpha subunit 106_00021 Putative exonuclease 106_00022 Putative ssDNA-binding protein 106_00023 Putative DNA helicase/primase 106_00024 Putative deoxynucleoside-5'-monophosphate kinase 106_00029 Putative DNA polymerase 106_00039 Putative tRNA nucleotidyltransferase/poly(A) polymerase 106_00041 Putative DNA ligase 106_00043 Putative dihydrofolate reductase 106_00044 Putative thymidylate synthase Additional functions 106_00006 Putative ribose-phosphate pyrophosphokinase 106_00018 Putative nucleoside triphosphate pyrophosphohydrolase 106_00037 Putative high frequency of lysogenization K (HflK) family proteins 106_00119 Putative superinfection immunity protein 106_00128 Macro domain (Poa1p like family) containing protein 106_00135 Putative C-terminal phosphatse domain of T4 polynucleotide kinase Hypothetical protein 106_00001 Hypothetical protein 106_00002 Hypothetical proteins 106_00003 Hypothetical protein 106_00007 Hypothetical protein 106_00008 Hypothetical proteins 106_00009 Hypothetical proteins 106_00010 Hypothetical protein 106_00011 Hypothetical protein 106_00012 Hypothetical protein 106_00016 Hypothetical protein 106_00017 Hypothetical protein 106_00019 Hypothetical protein 106_00025 Hypothetical protein 106_00026 Hypothetical protein 106_00027 Hypothetical protein 106_00030 Hypothetical protein 106_00031 Hypothetical protein 106_00032 Hypothetical protein 106_00033 Hypothetical protein 106_00034 Hypothetical protein 106_00035 Hypothetical protein 106_00036 Hypothetical protein 106_00038 Hypothetical proteins 106_00040 Hypothetical protein 106_00042 Hypothetical proteins 106_00045 Hypothetical proteins 106_00046 Hypothetical protein 106_00047 Hypothetical proteins 106_00048 Hypothetical proteins 106_00049 Hypothetical proteins 106_00050 Hypothetical proteins 106_00051 Hypothetical protein 106_00057 Hypothetical proteins 106_00058 Hypothetical protein 106_00063 Hypothetical protein 106_00066 Hypothetical proteins 106_00070 Hypothetical protein 106_00071 Hypothetical proteins 106_00074 Hypothetical protein 106_00075 Hypothetical proteins 106_00102 Hypothetical protein 106_00103 Hypothetical protein 106_00104 Hypothetical protein 106_00105 Hypothetical protein 106_00106 Hypothetical protein 106_00108 Hypothetical proteins 106_00109 Hypothetical protein 106_00110 Hypothetical protein 106_00111 Hypothetical protein 106_00112 Hypothetical protein 106_00113 Hypothetical protein 106_00114 Hypothetical protein 106_00115 Hypothetical proteins 106_00116 Hypothetical protein 106_00117 Hypothetical protein 106_00118 Hypothetical protein 106_00120 Hypothetical protein 106_00121 Hypothetical protein 106_00122 Hypothetical protein 106_00123 Hypothetical proteins 106_00124 Hypothetical protein 106_00125 Hypothetical proteins 106_00126 Hypothetical protein 106_00127 Hypothetical protein 106_00130 Hypothetical protein 106_00131 Hypothetical protein 106_00132 Hypothetical protein 106_00133 Hypothetical protein 106_00134 Hypothetical protein 106_00137 Hypothetical protein 106_00138 Hypothetical proteins 106_00139 Hypothetical protein 106_00140 Hypothetical protein

In order to roughly know where in the genome the nucleotide sequence analyzed based on the bacteriophage nucleotide sequence belongs, the phylogenetic tree was analyzed using Virus Classification and Tree Building Online Resouce (VICTOR) (see Figure 8).

<110> REPUBLIC OF KOREA(MANAGEMENT: RURAL DEVELOPMENT ADMINISTRATION) <120> Novel bacteriophage specific for fire blight and black shoot blight and use it <130>PD22-024 <160> 1 <170> KoPatentIn 3.0 <210> 1 <211> 84405 <212> DNA <213> Artificial Sequence <220> <223> Novel bacteriophage Fifi106 <400> 1 ctgtgaacag gtaacgtttt tgtctgcgtc aaccatccag cgaggcattg acacgatgtt 60 accaatgttg ttaggaactt tactggtgcg gctatcaacc agaccgttac cgttgttacg 120 aacacgcttc caaccgatga tataaccttc agagtcaatc tcgatgtcgt tatgagacac 180 gaagccccaa agctgttcaa cagagttgcg tgacgggttc tccatcagtt tttcgaagaa 240 gctgaccaga gttttaaagc cgtcatcacc ctgacgcatc agttccagaa tacgctgtgc 300 aatcgttgaa cggatttcaa tagtaccata gaacagtttg tcacctttga tggtgattgc 360 accctgagtg aagttctcaa tggacttgcg aatgttcatc agagcaaacg cttgtttgaa 420 gtcaccagaa acaatagcaa tctgaattgc tttgaagttc ggatgagttt cttcaacaat 480 ctctgattca ccgttcagag tcagaacaac agaagatgaa gtgttgatgt actcaacctt 540 atcacctttc tccagtgctt ccagtaccgt gctggctacc tgagcctttg gagtctctac 600 agccttggct ttctgagcgg tcttaggggc ggctttggca gttgacttag ccttcttacc 660 agacttctta gaagtcgcct tagcgggctt tacagcggct ttggcagggg ctttaccgcc 720 agacttctca tgacgctcga tagcacgacc gattgaacgg gcagacgtac cgtatttttc 780 agcggctgct gctttggtca taccgttaga aacatctgcg aaaatctgag catcaatctg 840 agctttagtc atagtcattt taggtttcct tcacttcgtt taagaagggc tgtttccagc 900 cccgtttgta ttaatggttg tagaatctta cgccatcgtc gttaacatac gttctgtcaa 960 agtcgatgtc aagtgcttcg gctaattcta acagttgcac acggttgaag tcaataaact 1020 tcagcacttt atatttagag ttgaacagat tgtttttgtt cttctcaatg tctgcttcca 1080 tacgctgacg tgctttcttg atggaagtgt gcagcttaca gtttggcagc atcttgtaaa 1140 tggaaccaag gatgttgctg tgactgactg agcaagactg taccatctgt ggaatcgaat 1200 agcaaggctt attcaagatt tgttcaagaa tctgtcttgc accttcccac ttcagaacac 1260 gaccacgcat tgactgacca aagtaatcat taccatttgg cagagcaaca cccattgctg 1320 gaagacgctt gaagcggtct ggcatagcag cctcaatcat cgactggttc atagctttcc 1380 agagtcgagg atgtttgtca atcagtttac gcagaacatt ttcatcactg gcgttttccc 1440 aatcttcggg aatctttttc cagttggcct tattaaacac cacaacctta ccagtgatta 1500 acggtgcaat cagtttagca acatcagtcg cacccttacc gttaaataca ccgttagtga 1560 ttacgttgtt gtttgactga aggtacaggt gttcattctc atcgtcctga aggacagaca 1620 agtcaacctt aaactcttca gagttcacgt aaccagtatt gataaacccg ttacggtaat 1680 caccaatgtt ttgcttcttg tggtactccg cataactgtg aacccacagc ttcaccacac 1740 ctttctgacg ctttgcaggg gtgtagcagt cttcgtactt agaagccatg tcgaagtgac 1800 gttggtcttc tggtactttg tgacggttcc agataatctc tgcctcagcg cgagagtcaa 1860 ccatgcacag gtagttcagg ttgtttttag tggactcttg gaaagcaaag cccataccac 1920 gtaacacttg ttctttacca gttgtcttct cagtacctga acgggtgata cggtcataga 1980 caaagaataa tacaccattg aagaagtcat tggtagtctt ccaagggagag accttagcca 2040 tgtctgtttc aaggttgttc ttagtccaac gctcacgttt cgcccaagat gatgttgaag 2100 tgaggttgtt ccagcgatag caatatgctt cgtagttcat catcaaaggt actttctgac 2160 cgttcagttc cagaggctga ccggaagaat cctttttgta catctgattg ccaaactcat 2220 caagacgagg aaccagacgg gctgcgtcag cctcttcaac cagacgggaa atttgcttac 2280 ctttccaagt caggttctta ccgaagtatg caatctggct gtgactacgc agcttgcttt 2340 tcaggttaac ccaatgatga ccagctttcc agtagtcgtc aatatcatca aaaccttttt 2400 gaaggtctgc catgaagtca gaagagatgt ccttaaaggc atccagaata aacttcttcg 2460 ttgactcatt catctggaga gattcacgac tcgcagagat agacactgaa ccgattggaa 2520 cgtaaaccca cagacaacca atagaatcaa tcatagggct gttgttgata gctgacatct 2580 catcatcaga catcaccgct tccagtttga cagggtaacg gatagaaccc atcaccacat 2640 acaggaagtt atcaccgcga cgactgtaag aatttcctgt accgtggaat gctgtgtaaa 2700 caccagcttt ctcagacata accatagagt tacggtcgat atagttgatg tcaatattga 2760 tgttaggtcg tacagcaaat gtttcaaaga cgtaagcagc ttcacgacga aactcatcag 2820 tcaactcttg acgaacagct acacgaacag caaggccagt ttcctcaccc tctgccattg 2880 gcttttccat cagcttagtc aggttgggta taccgttctc tttgtatgcc tgataagttg 2940 tcaccttgcc gtcgaagtat gaacgtacag tgaacgagtc agagatagca aacggtgact 3000 ttgaaccgat acccatagca ccgatgaagt cattcgaatc gttcttcgtc gaagcaccat 3060 agttcaggta catgttcatg attgcttcat gagacagacc gacaccttta tcacgaatct 3120 caaagtaagg ttcaaaggtg gttggtaagt gaatgtcaaa cggctcatcg acattacctt 3180 ttaccatgtg accatcaagt gcattacaag ataactcacg gataactgca cgtgccttat 3240 actcataaac accagaagaa agtaggtcaa gcatctcagg ggtcatcata atctgtgcct 3300 gagtagtctc cagagtatta gaacttttct ggactgcttg atgttcgtaa ttcattttca 3360 tgtcgggatt atccttttga gttaaggggt gttgctcacc ccaaaacaga ttctaaaaca 3420 gttgacagat gactgtcaat catcttctcc ggttccttga cagaatggac atacacaacc 3480 atctttaacg cctgtaccat cacaatgctt gcagcatgag tctacacggt acttggcagc 3540 taatagaccg cccacaataa ccagaaccag taagatgtta agaccagtgt gaacgtccat 3600 tcttcttacc ttatgagtag aaaattatag caggttcaaa tactacctgc ccttttatattc 3660 tggaggtact tgcaaacttg ataccatccc ttttacgggc atcctttaag tacgctttga 3720 gacttccacg cgactgtttc tgcaacttct gagtccaccc taaagtgtct tcacatccct 3780 tcacagtcag gacgatggtc actgtagatt cttcgctcaa atctttccag ttatcagcgt 3840 tggcgtgtcg ctctgcaatt aactccagtg cttcaatagc cttatcaatc tcagattctt 3900 caagaacaaa gtgagcattg atattgaacg actgcatctt taaatccggc attcttcaca 3960 tacctcttca ggttcttcaa ccttcgcttc aacgtagcac tcaaacggtt cacacacaaa 4020 ctggtcagtg gtatcagcac ggttccagcg acgagtgaag ccagcctttt gcttcttggt 4080 cagattgtca aatgcacggc taaaaccata cagatgttct tctgaaattt cttcaacgaa 4140 ttcaccgtta aggtctacac ggaaaccgtg agtaacggct gcacaaacat actcatggcg 4200 acgagtccac actgataaca tcgcattgat ttctttgcga gtgaactcag tctgctctgc 4260 cagagtcttg tgaacaccaa tcgccagcgg cataggaaga gacttgttga acacgttagg 4320 gaattgtttt accagttcac ccatacgacg attgtagaag tcttttgata cggcagtgat 4380 gcgacgattc atgatatttc ctcagttagt tgtttgcttt cgatgtgatt actttaattc 4440 atttggtgtg gtgatgtcaa cagataaaat aaaaaaggac tccgaagagt ccctttataa 4500 attacttcat gtttttcaag aacttgtcac ggattgtgag aatattctct tcatagttga 4560 ttgactcacc atctgcaaaa atcacacgga gttcattcaa tccctgctgg ttcaggttgt 4620 cgtatgcttg acgactcagc ccatcaatca tgtgcaacgg ttcacctgtt ccagtgtcac 4680 gaacaatgat gtagcctgta gctgatttct tcgtaccatc atcagtgatt gggtcttttg 4740 acagtggata gttaatcccg tcaacagttg ccacagtcgc cttgatagca aagctgagtg 4800 aatcacgtgt cagcatctgg taagtgaatg aacccacacc aaacaccacg ttgcagcttg 4860 cgaatccacg ctctttcaga cgctgaagaa tattttcagc acgttccagt gtgatagagt 4920 caccgtaaat caggccaatg tgttcgtcca gaaccttgta acctttagtt gttacatcac 4980 caccaaagat tttccacagt gattcgatag caccttccag aatttcaatc tgtacatagc 5040 gaccaacttt gacagatggg cttttaatct tctcgtcaag gaagacgcag tatgcaccat 5100 caaaatcttc agcatcaact acaacttcat agccagcgtt cttgataatc tggcaatcat 5160 ttgcagtcag cttgtcaatg gtcagttctt taatgtcagc aagattgctt tccagcaccg 5220 aagcccaacc aaggcgacct gatttgtact cttcagcgat gactgctttt gcactctcaa 5280 tctcaataca acgatagcca gcaacgatgt gctctggaat accggaatct ggacgaagaa 5340 ccagtttacc atcacgtttc atgatgacat ctttcagttc acgcactttg gttgtgacgg 5400 tatcatacca gttgtttgtg tcaccaacca tagagagaat accagttggg tagacctctg 5460 tcagaagacg ctcaaagaac ttctgttcag cttttgcaag cagtgctgcc tgtgtctgag 5520 gattaccatc accgaacgca ccggaagata acaccatctg actcgcttct actgcgatgt 5580 ttgcacaggt aacactgtgt tcggttgctg gtacagaacc tgcgatgtca ctaatctgaa 5640 caccgttacc gtaaaactgt ttcacagacc acgttgcagg gaatgagtca gtccctttga 5700 agaatgcaag atgaccaatg ttattctgaa tatcatcgtg aacaccagac ataccacgta 5760 atgcgaagtc atgagcctga tagtcaacga agcctttgtc aacaccagtt tcttcagcga 5820 aacgttccag caatacacga tacttcattg cgatagttgc aatggtcata accttccaca 5880 ggttagaact gaacaggtct tccagatagc caccgaccca tgcaaaatct ttgtgcgtgg 5940 tttcgatagt catcacagga accttgacag gtgacacaga accctctggc agagcacgaa 6000 caatgatagg cagataacct aagtcgtgca gtgctgcaat gtggtcatga gtcagtgtgc 6060 ctttcccaag tacaccatcc atataagctg catgaatttc catgacaaca tctttcggaa 6120 cgttgaagaa gttttcattc cagaacttga caagccaagt atcacggaaa gaggttgaac 6180 cgaagtttgc aactacctct gtgccaccgg gaaaatactt cgcattacgt ggagtaatgt 6240 taagaaacag tgaatccaga cccgcagggt attggaattt atgaccggat ttgtaagcgt 6300 cacataccag agtagcaatc agtgtattgt tcatgttcag tttcctatta aattaatgag 6360 ccgtagccga caaagttaac accgttacct tcctgttcga aggagttcgt ggtgtaaatg 6420 ttctcaatcc ctgcatttcg cagaacttca gtacccttag agaatactcc gtgagtaacg 6480 tacaagtcaa cagattttgc accgagagtg aaaagttttg cagcagcatg tacaaacgtt 6540 gcgccaccat cacacaggtc atcaacaatc aagcagttct tgtctttaac aatacctgtt 6600 gacagtccca taccagtaat ctcaccagtc atgacgttgc ggtgttttac cagagttgca 6660 agttcacact gcatagcctt tgcagcagcg gtagctttct tctctgcacc tgcatctgga 6720 gcaacgacga catcataatc acgtaagaat ctgtgagtca tcaaagcctc aacttgaggg 6780 atattgtaca ggttatgat aacaccacct gtaacatcac tgtgagcgtc aatcgtcaca 6840 accatgtcaa agttcatgct gttgattacc tgaccaaaca ccttcaggcc aaaggcatca 6900 ccatgataca tgtgacggtc atagcgtgag aatggcatgt atgacagcat cagaatataa 6960 cgaggcttaa ccatgtatgg taagaagaac tggttgattg cctctttcaa ctgcatgatg 7020 gtaatcagca tattaggctc ataaccctga gtgtgaatag cgacttcaac agtgtttgaa 7080 ccattgatg cctttttcat acccataaca tccagactca caccaacctc tccagcaggg 7140 aaggtggttc gactgattgc tacattctgg tgattgactg ttaaactaat catgttactc 7200 tccaaatgtt atcatttcgt cttgtctttg acggtgttct tcacgtcctt tcggaggaag 7260 gtaagcgggg tcaaagttcc cgtatgacca atactgtacg acactatcac agcagttgca 7320 acgatattct tcttctaaca ataaataatc tgacacatcc tttggaacac tgtaaaaact 7380 tttggacaga cagtcaggac aacgcttagg tgataaatca tctttgttga agaaccacca 7440 aggcttgacc ttacaaaacaa agttataaac tgctttcatt gtacactcct gagaatcgta 7500 aagcatattg gcagaatcgt ttactctgca taccgttgat tgtagagtgc ttcacaaggt 7560 gttcaaagag gtaatcaacc tgttcgtcat aactcatcag ttcagagtct ggaaggttat 7620 ctccttgacg ttggttgtca ctcttcgtct ggatgaacca gttgcttaca tgatgtacac 7680 cacctttat aagtggtata tgatgaccta aagtgaattg gtgattcttg ttcaaaccaa 7740 actcttcatt cagcaccgtg ataaagttct tgaaaaagca atgttcatac agattatcga 7800 ctgtgtaata aggaaaaataa cgagcaataa tctttttacg ggtcaaccct tcatggttca 7860 gtttcatcca gtttgaattc tcacggtcat actggtgact gaacagaaag tcctcacggt 7920 cttgaaagtc aagattgaca actgacatca ctactgcaac gaactctgag tacatacatt 7980 ctccagaaac taaaaaggac tcaattaaga gtcctcatag taactaacca gcaagagaat 8040 tgcaatagat tcttaaacct cttcgtgctg aactttcact ttcaaattca aggtatgtcc 8100 gttcacgcat ctcatcaagg tactcgaacc atacacgccc attagatgat atttgaacca 8160 cgtggggcgt tgaggtctcc gtaatatttt cggtagtatt ctctggcttg ttcatgtgtt 8220 agtgactccc atttatcttt ttcaaacatg tggtcaggtc ggcaatagca ctggcaaccg 8280 tcaatggcaa cgtagacttt accgttatac cattcaccat catcaccttt aatcttggca 8340 tcacctgaca tgacatagta cagactttca cgtggtttgt aaaacagata ttgacgagac 8400 atattcacct caaataaaaa gggactccga agagtcccga ttaggatagc tacagattaa 8460 cgtgcttcga agtcttcaac catcgcatca aagatggtcg ttgcacgtgc tgaaccgtaa 8520 gtgcggttag tgttaacacg cagtacagca gccagagtcg cttcaggttt aaccagaaca 8580 ccctgccctt cggtcagtga cagcagaaca tcaccgatga agtcttccat tgggtcttcg 8640 atacccttct ctttggtcag cttcagcatc gcactcagga ttgcaccgtc gattgctttc 8700 tctggttcgt agtttttcag gattccgtca aggaagaaaa tctcatcttt gacatcatcg 8760 tagataccta caccgacctt cacagcttta ccagttgcgt atgataccgc agacttgata 8820 gatttgttca tggtcttgct ggttggagca gcagacgtat caagttcggc tttcgcttgg 8880 aagtccaagt cacgagaaga catcacagag aaagtggagt caaactcatg gtacagttct 8940 ttttcgtgtt caacgtcacg aagtttgtag atttccagaa ttactggagt ctcatcatcg 9000 ttctcgaaga acaggttgcc agctttgtaa gctgcgatac ggctgttacc atccagaatg 9060 acagcggtct tatcatcgta tacaccgata cggaagacgt gctgagtagc agatgcaaca 9120 ccgttcaagt gctctttctt cagacgtgat tcagtctcac gctggttaat cgctggacga 9180 actttcagaa tgctgccaag tttctgagta acaacctgtg gcagttcagg gaacgtcagg 9240 aaggtgttaa cacgtgcgat gtcgatgtag acaggctgtg ctttaccaac tttgccgtca 9300 cccttatcag tagcgcgggt aacacccttt gcgaaaccga taacgccatc ttcaaccaga 9360 ccagccagaa caggtttgat agccttctga gttgcctgtg cagacacaac gatttcatca 9420 agagtcgcac cgttaggagc ggttacagca tcggtgattg cagttttcag ggcttcggct 9480 ttcagcagtg cttcagcttt tgcatcagtc tgagtgttag ctgcatcttt aacttcagtt 9540 tcgacgttag tgttttcagt agtcatttct cggtttcctt aatatgttgt tttcgtatgt 9600 cttgttgacg tgagtaatag tatagaaatc ggttattcgg gtcaacaact attttaataa 9660 tttttcattt atttataagt tgttgtttta tataaagaaa aaggactccg aagagtccct 9720 tgattattac gagtacgtgt agcactggtt tgataagcgt ttgctatgac ggtcagtgtt 9780 gtctggaaca acatcttcac ctgtcatggc tttggcagag agttgacgat tcgtctggcg 9840 ttgaatctca tgctgacggt ctttgacacc tgaagagatg ccactcatgc gtttcaattt 9900 atcaccatga tacacagcgt gaccatgacc ttctttacgg tctggattct ggaaccagtc 9960 ttccttacct ttcaagttct tacggcgaat cgtcttaccc attatcaatt tccttttcgt 10020 cttaataagt aaagccagct tgcgaccagc ttcactacat agatgacaac tacaatgtac 10080 cagaatgcaa tgcaaactac atctgaagaa tcaacatggg tcttcttcaa cccatacttg 10140 acattcccgt agaaggttga aatacctgcc acaactgaca ggacaacaac ccctacaatc 10200 cacagaaaga aagcaatttc cattcacacc tctgagaaag actactccat cagacgtttg 10260 gtcaggttgt ccacagagcc aatcttgttg attacattac cagaaccttc atcaacttcc 10320 acgatataag gaagcatacg aacaggctca cccgctacgt cccacagttc ctgcacctga 10380 tagtccttat caagtttctt cattacaaac tgctgaccac gcatattcag catcgcaaca 10440 gccttgtcac aattcggaca caaatcttta ccgtatacaa taaaactcat tagtaagccc 10500 ctaagccttt tccgtcttcg tcatcgttaa ccagagtgtt caacagatac tgactgttgt 10560 cttgctcttg gtttgcattc tgctgtgaat caatgttcat ccacttatcc ataaacggaa 10620 gcgggttctt cacaacaact ggatagttgt aatccagatt taagaaatca tacaatggtt 10680 tagctgaata gtaaacccat tctttcaaca gagttgggtt cagaccaaca actgagcgac 10740 catcgctgaa aatgtaatca gcccaattgg attcattctc catcacttca tcaaggatgc 10800 gctgaacctc acccttacag ttctggtaag attggaacca aacagggtct ttcagaagaa 10860 tgtccagtgc tgcgaagtcc atcttggagt gaaggatttc atcaagcatg attttctgaa 10920 caagctgtgc aacacccatg aacagaccac gttgagccag agcaaaggta catgcaaagc 10980 tggcaataaa ctccagacct tccagagcaa gaatgctgac catgaactta atcagtgtct 11040 ggcgaatatg ctcatcgtct agttcataac caagacgctt tttagcaccg aggatttcaa 11100 gttctgccat aacttcatca attgttgatg agcggtcaag aacagcctga ttatccatca 11160 cgcgcttaat catctcagtt gggtcactga tacactggcg aacgatttct gagtaggtca 11220 gagcgtgaag gttttcaatc tctgtctgct tagtcaccat agcagcaaac tcgttgttac 11280 tgatgaatgg ggcaagcaga gtgatgatgt tacgtgatac acgtgaatcg gcttcccact 11340 gccacagaag gttctcaata cagacctcaa tctcagactt agcagcggtc tggaagtcaa 11400 tacgtgagtt ctcaaggttc acttcatcct ctgaccagtc ctgtgctttc tgctgcttat 11460 acaggtcaaa gagttgtggg taaatcttgt tcattgagtc atacatagcc agaggctgac 11520 ccataaaacaa cgggtattca gcagtaaggt atgcgtcatt cttgtggttg aatacagttg 11580 aagacatctt gatttcctct taaaaagttt gggtcggact taaccgaccc ttgaagtata 11640 tattaaagtg agcagccttc gcaagccatt tctttcagtg aagactcacc gacaccaaca 11700 cggctgttca tgtaatactg tgtcttcata ccaagtctgt gagcataaat caaactctgg 11760 agagcagtct tagcagacac cttattacct gtcttgtgat aatccatgta gaagtcagca 11820 gagatggcct gaccagtaaa cttctggaag attgcgtaac actcaatcat atccttttca 11880 ggaacatccc aagcaaactg gtagacatca ccaatcactt cagcatcagg tgcaaggaag 11940 aacactgaac cttgtggtga cttcttgaac atcttcagtt cacgaacagg gtaaagtccg 12000 ttggttgtat tcgttgccag agatgaactc tcgtttggca taaacgcttc cagtacactg 12060 tgacggatgc caccgttggc tttaatgtcc ttgtgaagct gaacccaatc aaactgtaag 12120 gttgagtcta caacattatc cacatcttgt gaatagtctt caagaggtgt ccaaccgtta 12180 cggtattttg acacttccat accctcacac acgccacgct ctttagcaag acggagactc 12240 gcacggtgca gagaatagct atgaagttct gctaagttgt gcatcaactt cttacccttc 12300 ttagaagagt agttggtgaa attgcttgcc agatagtgag caagattagt gataccaaca 12360 ccgactgaac gacggtactg tgcagacttt ttcagggctt cgtatggata gtccataatg 12420 tccatgacgt tatcaatcat caggagggtg tagtaagcaa cttcctcata ctcttctggt 12480 ttgacacgtc ccgctacgat gcttgagagg aagcacagag cgatttctcc cttagccttg 12540 accatcgggt tataaagttc tgccatgctc tcaaaaccct ttgtaggtag gcagatttcc 12600 atacacagat tggagttcca gatagtatct tcgaacggtg tatgagtgtt catgttatca 12660 gggaagaacg gatagacacg accagtttca taacggttct tgataaatac cttgctcaat 12720 ttacgagcac tcacaatctt cttcttgatg tctggattgc gcatcacacg gtcatactca 12780 acctcgaaca actcataaga gccgtacatg cgtttgtgca attctggagc atctttgaaa 12840 gagaccagca tccattcatc atccttatca acacggcgaa ggaatgactc attgatacca 12900 acttgataat ccatgaactt caactgtttc tgtggaggtg tcacagggtt attcagtcgg 12960 agcaggtcat caatctcagg gtcaaggatg gtgaaaccaa ctgttgcaga gccgccacgt 13020 gtctgttgct tattagcctt tacagccgtg ttgatgcaag caaagtaagg tgtcttaccc 13080 tgatggacaa tagtcttgtt acgaacacca tcacctttag agcgggtctg taaaagaata 13140 ccgataccag cttgcttaca agtcatcgta taagcaatat gttcagcaat accgatagac 13200 tctgcggtgt cgtcaccttt gattacacag catgaggcat aaccactctt cttagaacgg 13260 aggccgttca ggtaaggagt tggtgcgtta atcttcaggt ctgacaggta ttcatagagt 13320 ttgataacgt cctgaatacg gcgttcagtt ggctgatgct gcatgactgc caaggccata 13380 cccatgaaca tgaactgtgg tgattcataa acacgctttg tcaaggcatc cttgatagcg 13440 tacttgtcac ggaactgttt gacaaccgca taaccatagt tcaggtcttt gtagtggttg 13500 atttgcatac caagatagtc aagctcagaa tcagagtaac ccatgtcttc ccacagattc 13560 agtgtacgca ttttgtcata gaagtctgtc agggttggga ttttacggaa cccaccaaag 13620 gcttctttgt agacattacc aatcagcaga cgaccagcca tgtttgcgtg tgcctgagtc 13680 tgacggtcga tacaggcttc aatcatagcc cactgaaggt cagaagttga acaaccatcg 13740 tgacaacgct taaccgcatc aacagcaatc tcagtccagt caacaccata gtttgcagcc 13800 cactcagccc aacggttaag acgtgacggg tcaaaaggtt gtacagtacc atcaaatttc 13860 tttacgttgc gaatcattat ttagcctcaa gttgtgcttt gaatttgttg ctcagaatct 13920 gatattgaat caggagggga accattacag cgaggattgt cttattcatt attttcattc 13980 cagattaaaa aggactccga agagtccctt gtgattaata tttgaagatt gtaccatctt 14040 cgtaggctcg acggaaggtc tctgtgacct taaccagcga agaagggctg attccacggt 14100 aagatgaagt caattggata taaccacgac tcttaccctt tatagtgctg aagcccattt 14160 ctgaacgagc acctttcgag tgaacaacta gagggtagaa gactaaatcg ccctctctca 14220 gttcgttacc gaattttatca gtgccaagga tgctttcttc gtcagacgat gacgtttgag 14280 acttttccat ctttcatcaa ccctttcttt acctttgaaa agagcaccat ttacgcgctc 14340 accataacag tttcgatgtt cacactcttc atagacaatg cctttgtcga ggtcgaaacc 14400 gtatttgaac agaaccgctt tcagtaaatc agtctgataa cttgcaagca ggtctggtac 14460 atctttgacc agagggtcac gcatcagtac agtgattgaa acgctgtaag tgcagtggtt 14520 agggtttttc aatccctcaa tcagcttttc cattagcacc tacccctttc aaccaattgc 14580 tcacgaactc tttatcgtga cctgcaccaa ctgacaatct taccacggat tcaccgtcaa 14640 agaaagccac acaaccaata tcaagaccaa cctcttcagc gaactcaata tcagcttcag 14700 agttatcaag gtcaagtacg gtgaacgaat aaccaaggct ctcaagagtt tttgccagtg 14760 atttacacag tgtgcaagac tcttttgatg gcacaaagat ttctgtatta cctgcaacca 14820 aatcatacag ggtcactgtt ggatggttaa ctggtttcat aatcttgtta tcgctcagac 14880 gatggacggt gtagtaaacc tttccattga cctcagtacc aacaacttca caagcagtct 14940 gagggtcttt ctcattgtgc tcatccaacc agcgaagtgc tgtaccagca tcttcagtga 15000 ttttcaaatc gttgttgtca caaacacggt tcagacctgt ttcatggtca agctgtgaaa 15060 ggtaggtgaa accttgcagc acgaagtcca tgtcaacctg accgtcaatc agacctaacg 15120 catcacgctc acgaatggct tggatagtct ctttcaactc ttccatcatt cgttcagctt 15180 gattctctaa tgctttccag tattctggtg agtagcgttt cgcagcattc agaccagcac 15240 ggatattcca aagatggaca ggattattag tagttacata cattcacacc tcaacattct 15300 tcaaagaaag cgggggagtt aacccccccga cattttatta ttattctaca tcttcgtact 15360 gtttgccagt aaccagatgc agggctgcgt gtttctcttt agcggcttca aggttgaatg 15420 ccagatttgc acggcgggac ataagagcgg caatcgcatc gtcaacctga gacagttcct 15480 gcttggcgtg ggacagttcc tcacctacag agtcaatcag cttggttaca gcgttgtcga 15540 tacgggtatg gattggttgc tccactttct taacgtcaga gaacagtttt ttcagaattg 15600 cgaacatgat gagcctcttt atattcaatt agttgtttaa ttcgttttgc tgctacaggc 15660 ttaatctctt ttacgaggaa cttgatagca gcgacatttg cattgtaaaa cttacgagta 15720 tcagagtcaa cacttttttg catcacatca aacttatgtt gaaggttagc ttcagcgtag 15780 accagacccg atttagtctc atacgtttcg atgatataga actcaaagtt gtctaaccca 15840 agctcttcaa tatctttctt aacgtactca gaagatgtga agtatcttaa ccaatctgaa 15900 tccttgatgc aagtctttct tcgaactttt ccaacaatct ttttcttagt aactgaaaag 15960 aattgtttct ttccgatgta aaatctgtta tgggctttat tgtagaccac ataaacaaaa 16020 ccgaaaagtt tttcagggtt aatctctccg cagagagaca cccaatgtgc gtaacgtggc 16080 agcttatcca ttcatgttaa aactccaatc atatttttca aaggtaaata catcgtccat 16140 gctacgctcc tgataaggca acatgaaatg ctgtgtcata agctcttcag gcgtcctgtg 16200 agtctctaca ccgtcccatg aggtatatgt gtacccttca gggaacttct tcttgtagag 16260 ggcatagatt gcgtctaagc actggtgaat agtttcacat tcatcaagga gtttgataat 16320 cgcagcacca ccaactccag agataccgta gtagccgtct gaagggtctc cagccagagc 16380 ctgataacag agccatttaa aaccacaacc aacatacttc ggaggctgtt taggttttga 16440 cttgataggg catcgccaaa cgtcaccaag tgggttatca acggtattga agatgagcgg 16500 agccttgtca taggacatgt caagcatgta accacccatc aactgacgaa ggtctttgtc 16560 aagtgataag atgacagcac gtttaccttt acgttcaccc attgcgatta cagcggcatc 16620 agcctcaaat ccatcagaca gtaagtgcat ctgtgacttg tcaatcagat gttgtctggt 16680 tgcgtcaagg tgagttggtg tgaagcctga acggttgcct tgatactggt gttcaagacc 16740 tttcaagtct ttagtcttct ccacaccacg ttgtgtcaag aagcccatcc agtcatcttc 16800 atggacacca gaagccttga tataagcctg tagaacatcc tgagtagcct taatagcttc 16860 ctcaacagac agaggttctt tccaatcata acgaacccaa tcttgtttac ggaactcgat 16920 accaattgac tctgcaatat cttgttgacc atccaaccaa gacttagcat cttttgcact 16980 caggaatggc ggagactctt caaacgtctc agagtttgtg aagcgatact tagtcttctc 17040 aacagcaaat gccccacgat aagcgatagg gtctgagtca aagtatgcca gcttaactgt 17100 ggcaggaatt tttgaaaggg tgaatttact atgcttctca gcatcggtta aagccatttt 17160 gatttcctct taaacgaaaa agggtacagg gaaaccccca tacccattga ttacttagat 17220 gtcttattcg gttttgaatg gaacatcatc atcgtcgtcg gaatcatcgt catcaccata 17280 ctgagggatg tcgttgtcac cgtcatcaga atcgtcgtcg tcaccatcgt tagaaggttt 17340 gcttgaagac ttctcagaag atgcaccttt cgcttcttct tcgatgattt caccactctg 17400 tacaggttca tcgaaaccaa gttctgacag ttcatcagct tcaaggttac ggtcaacaga 17460 agaaccacct tcgtaagcta ccaagtcaga gatgtaaacc tgctggtgaa tcggtttggt 17520 tttctgagca ccttcgaagg tataagtata ctcttcacag atgatagtac caaatgaacc 17580 gttaccgata gccagattag gaacgatacc gtcataatcc ttgcgctctt cgtggtcagc 17640 gtgagccttg gctttgattt tcttcatcgg aacgccaact ggtttacgac cttccagtac 17700 gaacacgtcc agaccatgag caccgaggaa gaacgagccg tcagggtaag atgcgtgacg 17760 agccagtttg ataatgctgt aagtttcagc ttcaaaaggt ggctcacact tgaacttctc 17820 ttcaaactct tcagcgtcaa cggtctcgat tttacaatca gagaacttct tcttgaacgc 17880 aaccgcagtc ttacggtcag ccagaacaga aacactgaag gtgcggttct tcatcgggaa 17940 acttgcgtct tcgcgtttac ccttgttcag ttgaggcatg gtgttcagga agtagaacca 18000 cacatctttc agagcataac gtggaacttc tttgcctttt acagacagag gcttcagaac 18060 aacaagttta gattcagctt tagacattag tcagtttcct tctatgggtt attagtttgt 18120 cttctcaaac gaggtgagta tcttaccaag cctacccacc tattgcaata cttattttaa 18180 aacattttac tcagtacgga atggaatgtc atcatcgtca ggtgcatcga aacctaactg 18240 tgatgcgtca gaacttgaaa cgtcctcttc atcctcgata ccgtattgca ttttaatgtt 18300 ttccatcgca gtgtcaagtt cttctccagc accagcagca cggacaagac gacctgtttc 18360 accatcgtaa taggcgtaac ctgcaatgcc tgtactttta ccatgacgac gacatttggt 18420 caacttaatc ttagtgatgt tcttacgaac acggtcagag tcaaccttgt tacgggtcag 18480 gatgaggtta ttcatagaaa tctggaagta tgcaccagaa cccttaatat cctcttctgc 18540 aacgtcacca ccttccgagt tagccttttg accgccactg ttcttacgaa cgtgacagac 18600 gttgacatga gcatagccaa gacgcttaca acgacgaaga atctcagaga gaacttcttc 18660 ttcatctgtc tcagctttgg acagcgccaa tgttacaggg tcaaggatga taacaccaca 18720 acccattgca ccaaccagat agtcaataaa ctcaagaagg ttatcttggt cgattgcacc 18780 ctgatggtca acaatgttga tacgacgacc ttcagaaagc tgtttgtgtg cttccttgat 18840 ttcatcccaa ttacgttcat cgtatggaat ctcagaaatc tgcttgctaa ggtgaacaga 18900 gcaaagcatt tccatgagtt cttcataggt gtcttcacaa gggataacac caatcttctc 18960 atcagtctgt gtgaatgccg tgtaaatcat ttcacgtgta aacgcagact taccaacaga 19020 agacggtgca gcaatcgtcg taatctcacc tttaccataa ccaccgaaag tcagtgcgtt 19080 caagtcaccg aatgattcag ggaatggaat cagtggaacc ttaccacgat tcttcattgc 19140 gtcataacca tctgcgaagt tcttgatacc agcaggacag taacgttcag cgttgtacag 19200 acgatgtttg aattcgtcca gcatcacttt acgtttcttc tggctaccct tccaccaatc 19260 agagaggtct ttgatattct ctggatattg gaagatacga accttctcaa tcggggagaat 19320 acgagcagcc tcacgcataa acttagcacc agcttcatca ttgtctgcac agatataaat 19380 ctcttcaaac gatgtgatgt actgatagtt atccttaata cccttgatgt ttgcaccaga 19440 aggtaatgag gtgtgtgcat agtgcttacg gcgattcttc tctacaatca tactggtcag 19500 gaggtcagcc gtcatagact caatctcacc ttcccaaatg aatagacgct taccaccttt 19560 ggcatgacgt tgtgaaccga agagttgtaa ctctccagaa atatcaccaa caccaccgga 19620 gaagtctttc agcttgccac gcagttcagg tttcttgtag tcatcaggga atcggtgacg 19680 tacacgataa ccagcatgat aaggaactcc agcgttatca agtttatacg ttgggtagaa 19740 gtgagcgcaa atatcaccgt taccgtcagt gtcaaccttc acaccaaaag actctaacag 19800 ggcttctgga agaacacggt ctggtaaatc catagcaacc aagtcttcct tgacagcagc 19860 catatcatac ccttcgaagg tacgtttagt gttttcagtc ttttcataag ttctttctga 19920 catctgattc ttctccacat cccattcggg aaaacctttt tcacaagcaa aacaagtcat 19980 tgaatggcta ccgtcatcat gacgataaac cgaacctgca tcagatgagc cacacttcgg 20040 gcaagcagta tgcccgatga atgtaccctt ctctttctct ttgaagcgtt tattagtcat 20100 taatcctctt cgttctcttc aggtattctt cacgaagacc tttcacaatc ttatcagcct 20160 caacccaaatc ctcatggagt tgattgtagt aagcacggtg acaaccttca cggatggact 20220 tctccatctc atcttcaatt gcagcacgac actgacgagc gatgttgagt cgtccacaaa 20280 tgactgaaat tggtaataag ccaatctctg ctttacggaa gtcaacaaca cgtgtgaagt 20340 gtgatactac accgtacata tcaaggactt caacaagaag tttctcaagt tcatcaagcc 20400 cacggctatg gctgttgatg atagtccctg acagatgttc tttactaatg ccagcttctg 20460 aaacatgagc agacacttga gatgcagtca cctctggatt gattaccttg agaatcaagc 20520 cgctgtgcat cttaccaaac tccgcttcat tgtcaaagcg caaatcactg ataatggtga 20580 ctttgttcag tttagcagcg ttctcatacg ctttcttagc aaagttaatc cagatgtcat 20640 tatcaaagct ctggcgcatc agttcagtac cgtaaagctg atacagtctg cgtggagaga 20700 ttaatgctga agaaccttca acctcgcttg agaactcatc ttcctgaatc aggtcaatga 20760 aatcacatgc aagtgtgtga cttgtgaagc tcaggtcttc aaactctttg tagggtgaat 20820 caggatgttc gagagttttc aggatagcag atgccatcag gtcaggattc atacgaatca 20880 taacaggggt ttctttcaaa gacccgtcac ggtgacgctg gtcaaaacca aacataccat 20940 caacagcacc tttgagtggg tcatagaagt ggatgatatg ggagtcacga ccgttggctt 21000 tgagaatctt ttgcatgagg gatgcacacg tatctttacc ggaacgtgca gcgccattta 21060 acagaattaa atcaaccatt tatagttttc cttataggct aaaaagcccc aattaagggg 21120 ctagtaagat aacatcagtt actggtcaag tccagcattt tgaacttcga atcgggcttt 21180 ctcgctgtaa cgcttcgaga gagtagccaa ctctgcttcg aactcatcag caccagcctg 21240 accaagtttt tgcaccatat ctttcttgat gcagaaatcc ataacttgct ccagaagctg 21300 actgaacagg aatgcagtca ttggagcgac ctgctggcag aaggtgtcaa agtccggtgc 21360 aataccttct ttcatcagat agtgactgtg aaccagaact tcggtagaaa cctgaaccag 21420 tgcagcagag aggctgactg tgtagacttc agtaccttga gtctgagcaa agaacacctg 21480 catagattca ttcagttcct gcaaggtctt gtcatagtca cctgcacctg tcttttcaac 21540 aaacgcagca gtggactcat tggctttcgc taaggtctcg cgtaccagtg tctgatactg 21600 tgcatcgtca gactcatcca gacctttcag ggcttcagag tatttggcga acagttcagg 21660 gtagttgtaa ggctgaccag caacccacag accccatgca gccatcagcg tttgacaggt 21720 tgcaatcatc agtttacgag caccagcaac ctcaacctcg ccgtaagcgt tttccaaatc 21780 agcgactgta tcggtgtcgg tgttctgaat cttctcagtt gtaccttttg agataatctc 21840 cagaagacct gatgcagaca gcgcaaccat aaacggaact tcagggttgc cagttggctt 21900 gataccaaac ttatcagtca gagttttaag ttcttgtttg aactctacct gaagttggtt 21960 catggtctgg tcaagctgtt gttctttagt catgagactt ttcctttttc tggatgtatt 22020 ttaaggagaa cccttttgag ttttccatat tggtgtaaat cttatcgagt aagtcagatt 22080 tcccgactgc ctcttgatag tcactaagac tacgcgactc agaagtgtct gtcaaccaat 22140 aaatcgcagt gaataaaaaa ggtgtaatcc tctcaatagc atacgcttta gtcacgtaag 22200 aatcaggggc agactctgaa tcgaaccacg tttcagtgaa agcctttgca ggggaatcac 22260 cgataatttt aggtgcaatg tcaaagaaat ctttcatgga ctcttcaatc agaccattaa 22320 gagttttgac atcaggaacg tcataagagg ctggtgactt catcaccttt gcaaactctt 22380 tccagaactg ttccttatca tctgtacgga ttgattcagt catcccatac atacaggcca 22440 tgaaatagcg caaccctttt gaggggtcta tgaagtagtt taagtatctg ttcggcttag 22500 acagtggcgg taactccaac atttcattgg cttcttgcaa gacatccatc tgactcaata 22560 tcagacaggg aatcagtaaa gggtttctca atttagtcat ggttgatacg actctctctt 22620 aatttttcag gagactgaaa gttcaaagac ttctcagcac gtggggtata gtggcgactc 22680 cactctttac cggattcttc taagaatctg gaaaggtctt ctacctcgat acctgtacga 22740 agcatcttac gcaacatatc catgatgagc aaggtatctt tgtggcgtct acgctcttta 22800 gagatttctt tcatctggtc gaagaccaaa actttcaaga catcttcata gtcctctgac 22860 aattcaatct catgctggac atcgaccata cgagcatggc tatgtgaata agccttttga 22920 gattcttttt ccagagctaa aacagcttcc agactttcga taaagttttg gaagactgga 22980 tgaatattta atttgttcat agccttacac cgtaaattaa ccaatagcta atcttaccat 23040 atcggcatct ttaaagtcaa tctataaaga ctcttattat atctaattgt tacttattgt 23100 tttctaaaga agtttacttt aaagaatctg ttaagaggaa cggcaaaaag aatgtacagc 23160 taatttttaa ccatgtcaag ttgtatttca ccatgcactt gtgatatgat gggtgtgtta 23220 ttaatcagag gataggggca tgtgtgaatt aactcattca gaattaagaa gtttatgaa 23280 atatcgaaag acaactggtg aattcatttg gaaagattcc ggtgaggttg ctggacattt 23340 atctaagtcg ggttacatcc agattaaggt gaacagtaaa acttaccgcg cacacttact 23400 ggcttggtac tatgtcactg gtgaataccc gattaagata attgaccata agaaccataa 23460 gaggtatgac aaccgttgga agaatcttag agaggctaca cacgctatga atagctggaa 23520 cacttctctg cggtcggata gcaagtctgg tgtgaagggt gtctgttacg atgaacgtac 23580 aggccgctgg agagccagag tgtgtgtctt cgggaaaacc tatggctgtg gctaccatgc 23640 gtctaagagg gctgctgagg cggctgtaag aactcgtcgt gaagagttgc acggtgagtt 23700 tgcttgtcat gggtaaacca tgtatcatgc tcgtatctaa ataaggagtt gaaaatgcct 23760 gagcaagaat tgttacacgt tgtaggtgac tcggaaacaa acggcctgtt aatggagttt 23820 gatgacgttc acgttatggc gttctgtgag tacaagtcta aaactgatga ccaaatcttt 23880 gtattcactg ataaacctgt accgattgga cacccgtaca ctaagtacat caaaggtggt 23940 cttcgtgagg gtattgaatt catgatgaac cgttgtgcag aagttgttat tcataacctc 24000 tgtcaatatg actggtgggt gttcaaccat attgcacctg acctgttcaa cattaacact 24060 gcaaaacctt gggatgccaa atggcaagac tcccttgtac agtcgcgtgt tcagtggcat 24120 gaccgtccaa ctccacgtgg ctataagggt gctcatggtc tggctgcatg gggtgctcgt 24180 tgtggtgttc ataaacctga gattgaggat tggacaactt gggatggacg gatgcttaca 24240 cgtgtcgttg aagatattcg aatcaacgcc cgtaccaaac gtatgcttga taaagaaaag 24300 cgtatgctca aggagtgtgg tgtagacact acagagacct atgaacgtgc caaacaaaca 24360 tctttctgga tgggtcaaca gggtatcaac ggctggaaag caaatgttga actgatgcga 24420 aagtatgctg acgaacttga tgtaactatt acagagttgg ctgaaaccat cgagccaaac 24480 ctaccaaccaa ctatcaagaa caaaggtaaa gtcacttatg aggactttgc taaaggttgg 24540 tcaacgtatg ccgatgagtt aggtgatgaa tctgttcgta agattacaca gtacccacca 24600 acgaagtatc ggatgatgaa gcgtaagggt gagactaaac gtgttgagat taaaccgttg 24660 gctaaaccta ccttcagttc aaccaataag aaggttatca agacttactc tgttgagaac 24720 gttgtaacag gtgaagttgt taagaccatc tttggcagtc tgaaagaagc acgtgatgct 24780 tgtaaagagt tgaacggtac agctaaacca aaagattgtc agtggaaagc tctgaagcgt 24840 gagtcagaag gtcttgagta tgaacacgcac actgtgaacc acttcgggct tgagtctaac 24900 cgttacgatg gtgtgattgc tgcacctcat acacgaatca gctttgagaa gtcaaccatg 24960 tcacaacatg acgtagtgaa gggttatctt ctgaacaagt gtggttgggt tcctgatgac 25020 tacaactaca agaaagataa ggatggtaaa gatgctaaga tttgtcgtga taaacaatct 25080 ggtcgtctta tccgactgaa caagttcacc cgtaagtgga aagatgaatt tggtcttgat 25140 gtgattgagc atgacggtat caagtatctg atgtacactt acactcttcg acgctctatg 25200 ccagaatggg acaatgcgcg tttacgcact tctcctaagc tgactgaaag ttcatacgac 25260 acatcaacg gttcacttgg acagagtatt gcgaagtata acacgttaca gcatcgtcgc 25320 cgtacactcg aaaaccctaa gcatgatgag aagggttggt tgaacatggt tcgtgctgat 25380 ggtcgtatga gtgcaggtgc tacagtcttc ggtacatcta ctggtcgtat gactcagtgg 25440 ggtatcgtaa acacaccttc atctgctgcg gtgtatggtg ctcagatgcg tgaagtgtgg 25500 ggctgtgaag agggtacaag cgttatctct gtagatatga actctgcaca gcttgtgctg 25560 ctttgtaact tcatgggaga ccctgacttc acctacgctg taacgaaagg taaagaagat 25620 gtggagttca agcgtacaga agatggtcgt tactactgtt ctaaactgga caagtacctt 25680 gacccgacga ttgacaaata cctgaactat gatgaagaga acgacttgta cgttgtctac 25740 actggtactg atgctcactc gtttaactca attcagttta ccctgaatga agagtcagat 25800 attatcactg cacgtgcaac gcaggatgaa gacctgattc ataagattag taacggtcgt 25860 aagaaagcga agaacggtat ttactgtctg ctgttcggtg gtggtgatga gaagtttgct 25920 aagaccatca aagtgaagac tacagatgaa ggtgctcgaa tcaaacagac atacttcaca 25980 cgtcttccga agattaaggc tctgcttgac cgtcttgaag atgagtttaa ggctactaag 26040 aaagctcttg gtcaggtgtt tggtaaaact cacccaatct gcaaaggtgg ctttatcagt 26100 gttgcaggtg cttatctgtg gtgtaacagc cctcataaga ttctgaacta tcttttgatg 26160 ggttccgaag ctcaggttca gaatgaggca gtgaattggg cttgtcgtca gatgcttgac 26220 aaaggcttga tgaaactgaa tggtcgtaag ccagctatcg gagcaagact gctttgtgcg 26280 tatcacgatg aaaactcatg ggaagttcct aacgagatga ttcatgaggc taaagaggtt 26340 attgatgcaa tgtacggtac agcatctaaa gctctgaaac tcttaccgga cacactggta 26400 acaggtactg gtaaagttgg taagaactgg ctggatgttc actaacagtt gacatgatgc 26460 acaaggatgt gtatcttcac acacaaatag gtagatgatg gaaaagattt ttgaagttag 26520 ggtaaaagac cgtgaaggtt ttatccgtca caataaaaat gctggtcgta tggcaatccg 26580 tatggcagca attctctgtg caaaaccatc tttcaagttg atttgtcttg agaagcatgg 26640 tggtgtagat atggttagaa cgagtgttga gactcttgag ggtactatcg ctctggctat 26700 ggatgaaatg caatttatgg aggtcgttca atggaacgga aaaatgctat gtttcaaccc 26760 ttgagtccag atgatattgt ggtctggcct gatgactcat ggtgcttccg gtcagaactg 26820 gaagaataca cttggaagag tgatgattat cgctggattc taatctctga ttctgagtat 26880 gattccttca tgaaatccca agagtttgat gaaacagatg aagccgaact attaaagact 26940 ccacagggtc gaatggcttt agctgagatg aaaaggggtc tgtgatatgg gttacgggat 27000 ggatgatgaa gtttgggatt atgctgatgg gattgctgag gaagaaaggc aagaacgttg 27060 ggaagatacc gaacgaacca gaaacaagat gaaggtgcgt tataaccgtg ctaagacggc 27120 tgaaactggc gctaccatct cttgcccttt ctgtggtaaa ctcattgtga aaaggactta 27180 ccataaaatc ttttgctcaa atgctaagac tcatggtaaa gttaactgta aagaccgatt 27240 ctggaacttc gctgatgaga accgttgttt cagaacacaa ctaatgggaa aatgatgaac 27300 agtgtgattg accctgatga tgtaccaagt gacgttgaaa tggcacgtga tattgtcgtt 27360 tcatgggact gcttcggtca tgactcaatc tttataatca acgaagaact gtttcgtcgt 27420 atgatgggta aacgtgtcta ttttgaggca ggtaaatcca aagtgcttcg tcgtcaggac 27480 ttcaatattc ttgatggatt ctctgagaaa agcatcgcag agatgaatca caacattggt 27540 gatgatattc gaggttatca cccactacac cttttagagg aatctgacaa tgatgaagac 27600 tatctttaag tttattatcg gggctatctg gctctttatc ctttggctgt ggactctggt 27660 tgtaactgac ttaccagact tcatgtcacg tgtttataca gcagagttct ttgttgggct 27720 tttcctgatt gccttgctag ggctgttttc ttataacatg tacaaggtca caaaagacgt 27780 gtggaaaaag ccatgactca tcgtggaaga aaccatgcag caacacaaag gaatgcgagt 27840 gaatcaacga ttaagaatcg tagaaagaaa ctctatgcac gtcttaactc gttgattgat 27900 aacgcaactt tgccagcatc tgagaaggtt ttcttgaagg gaaccatcaa aggtgtcatt 27960 catgaactga ttgatattga gtattataaa aatgagtcaa gaaatgaaag aaaaagcgtt 28020 gacatccctg accagcaaca ttaagataca cctactcgac gcgatacagg acatcaacac 28080 tggtaaagtg tcagatggtt tgaagactct cgtagagttg cataatttaa tggttgccat 28140 tgaacctgcc attgaaaaga tggaagagaa catggtacga cttggcgaaa tcgcctctaa 28200 aaggaaactg taaaaatgaa gaagctaatc aagaatatcg taatcgctgc tgcaatggtt 28260 atcggtgcaa cgtcactaac cgcttgcaac aaggtcactc ccggcaatgt cggtgtgtac 28320 gtgaagacaa ctggttcaga taccgataaa gacgctatcc agcgtgtcgg tcttggctgg 28380 aaattcactg cttggggtca tgacctgtat ctgttcccaa caactactca gaacaaagaa 28440 tggtctggct ctcaggcttt caaaatccaa ccttcagagg gtggtgatga gtggacagtt 28500 aacgttggtc tggcttacca tgtagaccct aacaaggctg ttgacctgtt ccagaaatac 28560 cgtcagggta ttgatgagat tacagacaac tacctgcaca acatgattcg tgacgcattt 28620 atccgtcatg cttctaagtt gagtgttgaa gaactgtatg gctccggtaa gactcagctt 28680 ctgattgatg tcaaagccga tgttgcaagt caggtagcag gttatggtat cattattgag 28740 aacatctact ttacaagcag cccactgcca cctaaagcgg ttgttgagtc aatgaaccag 28800 aagatttctg aacagcaaca tacagagcgc cagaaacagg ctgcactgac agcggtacaa 28860 actgctgatg ctcgtaagaa tgcggctcag ggtgagaaag acgctgcaat cctgaaagct 28920 caaggtgaag cagaagctat ccgtattcag ggtgaagctc tccgtcagaa cccaacagtt 28980 attcaactgc gtctggttga gaagtggtct ggtaatctgc cactggtaca gggtcaatct 29040 caaggtcaga ttaattgacat gtcagccctg attaagcagc aaaatcagta atttacaaag 29100 ggactcttcg gagtcctttt ttattgcata tttttcttga ccttagaatc cattactgtt 29160 aaattacaca cacattaaca acacagaggg ctttaagatg aagaaagaaa acgtgatttt 29220 ccataataac tatgcgatgt gtttctacgg gaaacctact gcaaatgaaa agaagatggg 29280 tgagaacctg ttgaagttct ttgaaggtaa gggtcatcct gtctacaagg caactcgtaa 29340 aaatggctac atgcaggttg gttcagctta ctccaacttc aagttggcct ctggtaagaa 29400 atcacaagaa gcccgtgatg aaatcgctaa cacgctgttt cgtgagcgtc acggtctgac 29460 cttcatcgag attgagattg aagttgacgg tactatctac ccgaccttcg caatctctaa 29520 agacgacatg cgcatccttt ttgagtgagg ttgatatgga tttcttttta gtaggtggtt 29580 caatcaggga tgcagctttt ggtaagcaat catcagacca tgacttcgtg gttgtgaacg 29640 ctactgaaga acagcttctt gcattgggtc tgaaaaaggt tggtaaatct ttccctgtat 29700 tcattaccga tgacggtaca gagattgcta tggctcgtcg tgaaaactct acaggtgaag 29760 ggtacaacga cttcgaagtc gatacaaatg gtgtcacact ggatgaagat ttgcttcgcc 29820 gtgacttcac tatcaatgcc tgtgcttctc ctgtaagagc gggtggtgca aacactgatg 29880 agttgatttg tgctcacccg atgagtcgct atgacttggc aaacagaatc cttcgtgtgg 29940 ttgatgcaga gcgtttcaaa gaagaccctt tgcgaatcct tagagggcta cgctttgcag 30000 cacgtcacaa cctgttcatt gagccagaga ctaaggctct tttccgtgag atggttgagg 30060 ctggttgtct tgctcatctt ccgaaggagc gtatttggaa agagattgaa aaggctgcat 30120 cttctgataa cttctggaag tttgtaagtc tttgtggtaa gttcaacttg ttcgctgaaa 30180 tctccggtat gcgtggcgta agacagccta agaagtatca ccctgaaggt gacgttctga 30240 accataccgt tcttgcatta caacattatg aagaagtgga gctacctaaa tatggatggg 30300 ataatagtaa tcgggtcatg cagtccctgt gtatcttatg tcacgacatg ggtaagagga 30360 ttactctcaa ggagcgtgga aatcttcacg gacatgagtc tgacattcgg gacttggagt 30420 ctttcttgga cagaattggt gctccaaaga atgtcagaag tgctgctaag ttctgtgcgg 30480 aacaacatca tcgtgttcat ggtatgtttg tacgtcgcgc taatagactt gcggtatggt 30540 tagaagaaca taaagagttc aataacccaa ctatcgtgaa tgctctttgt atgacatgta 30600 tttctgatgc caacgccaga ctacagaaag tcttgtacct tcagatgaac atgcttgctg 30660 aaattgtaag tcaaacaaga aagctctctc cagaggttgt tgaggctatc aaagagactg 30720 ataatattca gactaaaaag cagctaattc acaagtcaaa cacttataaa gttggtcaga 30780 ttcttgaccg ctacttgaat attttaaagg aatcaaatgc ttagtgctat tctggtttgc 30840 ctaacaatcg ctatcggtat tttatggctc attgtcgata gctttaaaat tctgaaattt 30900 gcaacggaaa gacccttgcg tttcgtatca gacacggtta ttatccttgt cggtatcgtt 30960 gaaggtctcg cagcaggtta catggttcta ttcctgtaca agctgttccg agcattcttc 31020 ttctaatctt actggactct tcggagtcct tttattctc taaggaaaca agatgaaaac 31080 tctcttcaaa cgcaacaaga acaaatctgt gaaccagtgg caatgctttg ctgaaggtgc 31140 tgaagtggtc actgtgtacg gtaaggttga tggtaagatg atgaccgagc gttatacggc 31200 tgaagctaaa aacatcggta agaagaacga aaccacgcct gaacaacaag ctattctgga 31260 agtcgaatca aagtatcgtc accagattga ccgtaaaggt tatgctgaga atcttgatgc 31320 ggctgagaca gtcgttaaag atggtgttat gttggctcat gatgctgtca agatttctca 31380 taagaaatac ctcatcttcc ctgctgatgc acaacctaag ctggatggtg tacgctgccg 31440 aatcaaacgt gatactctgg ctgaaaatgg tttcacagcc tattcacgtg agaacaccga 31500 gtatgtgatg ccgaatcttc tgattccttc tatccagttc atgctcaagt cacaccctga 31560 cgtgcaacag tttgatggtg aaatctatgc acatggctac gaccttgaag acatcgtaag 31620 catgattaag gacgctgaga accctgagcg tgatactctg atgttctatt ggtatgacat 31680 cattgacaca accctgaact ggcctgagcg tagtgcaatc atcaactcaa gcccactgct 31740 ggaatccgat tttaatctcc gtattgtagt cgtagagtcc acacgtgtta acagctttga 31800 agagtttgac aagctgcatg acaagtgggt tgttgagaag tttgaaggtg ctatgtaccg 31860 ttcaattgct gctgattctt tctacggctg taatgagcgt tcatacttcc tgattaagca 31920 taaaaagatg ctgaccagtg aattcatgat tgttggtgtt gagaaagata aacgtggaca 31980 tggtaagttt gtttgtttat catcgaaggg taagaagttc agttgtgctt ggaagacgac 32040 tcatgagaaa cgtcagtatc ttgctgacca tccagaagag ttcattggca agccgctgac 32100 tgttcagtat caaaaagaga ctcgcaaggg tcgtgttcaa ttccctgtag gattaacggt 32160 acgtgactat gaataaaaca ctgaaagagt tgggcgttta cgcccactat ttaatcatcg 32220 acaaattctc tggacggcct ttccagcaca gtacgctgac gagtgacctg acaaccacta 32280 aaaagaatat caccagctat caccogtaatg aggtgtggtc taatgagaac ctttaccaga 32340 cccttgaaga ccgtaaagag ttcgatatgt tcatggtgaa acttgatgct gttggtatcg 32400 ggtatgaccg tgctgttgat gttatcaacc agcgttatga acacgaatgg cagaagctgt 32460 caaagggtct ccgtgaacgt ttgaatgttg gtgtggtata tggtcttctc cagattattg 32520 agcgatttga gcgtgagatt agagagaatc ataaaaatctc tgacagtcag cttgtcgtca 32580 atgtgctgaa ggatttagtg gctttccgag aagtctgcaa agagttggtt gaggtgcaac 32640 cgattccgaa aaaggcttac aagtgggaaa agctgaagtc tattgaaatt cttgaagtta 32700 aggggttaga atttaaatga ttgctacagt ctatgctata aacagcgtag gtgcgtttgg 32760 gaactatgac ggttcccttc cgtggcctaa gaactctgaa gacctgatgc gtttctcaga 32820 gattacaaag aacatcggtt ccgtcctgat gggtaagtca acttacaagt ctctggttgc 32880 aagaaaagaa aatcagcttg acatccattt accacttcca gaacgtaacg ctatcatcct 32940 gacgagtgag ttaccgtgtg gtactaaact cgaattcggt gaggttgtgt ctttataaggt 33000 tgatggtaaa gaggttcatt tcatctgcca tgagaccaca acagctatca aactcgcaga 33060 gaagttcttc ggtgacgtag ctgtaatcgg tggctcaagt atcattgcag aggctttacc 33120 actaactgac caactctacc tgactgtcct tgacagcaat gaacaggctg atataatggt 33180 ggacttcgaa gtgcctatcc atctacagct tgcgtatttt gaaaggctga gaaatggaac 33240 tggcaaaact ttccactaca ttgagggatt actatgagca ctatcgacca acaatataaa 33300 ggtttcatgg caaacattct gtctaatggc gaactgagaa aatctcgttc aggtaacact 33360 atcagtgttc cttttccaga agacctgcgt atcgacattc gcaaagagtt tccattactg 33420 acctgtaagt ctgtccctat gtcactggtt gcaggtgagt tactttggtt cttggctggt 33480 aagactgact tacactctct cagaatgtat cagttcggtt ctgatgaagg tcagaacaca 33540 atctggactg ctgactatga acgttatctt gagtcagtcc tgaagatggt tcgtaaagtt 33600 cagactggtg atttgctgga taagactgtt gccaaaatca aagctcaaga agatttgggt 33660 atgatttatg gtagtcagtg gcgtgagttt gaatcttgtg agatgacctg tgaccagatt 33720 gaacgtttaa tcactggtat tcgtgataat cctgcgagtc gttatcacat tgtatcagca 33780 tggaaccctg tagccgttgc gaataatctt ggggcattac caccttgcca catgttcttc 33840 caagtgtatg cctctgaggg cggctatatg gacttacgct ggtatcagcg ttctgttgat 33900 acattcttag ggttgccgtt caatatcgcc tcatacgcgc tcctgcttcg tattctggca 33960 caaatgacag gctacacgcc tcgttatgtg attggaacct ttggtgacgc tcatatttat 34020 gagaaccatg ttccggctgt tatgcaggtt ctggataatc cagagtttga agctcctacc 34080 ttgaatctga ataacttgga tttgagttct ctggataagg tgttagcttt aaatggtaat 34140 acctttaaaa atgctcttca gggttatcaa aacgctggag tagtgaaagc ccctctttct 34200 gttggttaac agatacaaaa aaggctcccg taaaaaggag cctattaatt acctaccatt 34260 ggcatgattg ccgtcttcaa atttcccctg ccatctcgaa acgataccga acaggttatt 34320 aacagactct gtgagtttgt caactgaatt agccatcttg tcaatgttct tctgagaagc 34380 gtctgcataa gccttcatac tttcctgatt aatctctaac tggttgacct tagtctccag 34440 ttgacttact ctctgatggt cagcaacctg taccgtttca atctgagtga gtctgacatt 34500 aacagccaga taagaagcaa acactgacga acaccctaca atgattgcag caatgttgtt 34560 ctcaatgagt ctcttgattt ccattatgga ttccctccgc tatcagggtg gtcataagtg 34620 tcttcaagtt tatccaagaa ctcatcatcc ttagttgttt gtgtgcgctt ggcgatatac 34680 ttagccagtg tgaagaaaac gtgcttaatc atacgctctg tgaagagtgc gataagtagc 34740 tttgccccga aactggcgag tcctttaacca agcaacgcga agatgaaggg cattaccgtt 34800 acctcgttgt tggttagata taacaaaaaa cccactaaga aaactctcag tgggctgtcc 34860 atcatttaat aaactaacat tgaagggcta ttctgtcaac ctcactaaac gtcaatagac 34920 gctttgtagt ggtctgtaat ggcacaatct tggtagaagt tgtaaccaac accatgattc 34980 ttatagataa tgtacagtga taatccacgg aaaatttcac cagtataaaa tccaaaagac 35040 ataccattat cacctgatac tcttgggtct ggagaccatg caccgttaat agcctttgtg 35100 gggtcatcaa cgattgtgaa gttctctgcc aactcacctg cacaggaatc aataaagtag 35160 tcgagacctt ccacttttga acctgcgagt ttagagtaac aagcacctgc taaccaaagg 35220 ccgcacatgt gacctgtgaa gtcgttctca atccaaactg gttggtctgg tgcaattggg 35280 aagtcatttg gtgaatgacc gtcaaacctt tctgcaaatg agacaagcca tgttgaccag 35340 cgttcaacgt actccttcag gattctcgga acagtcttcc ctcttacaga aagctcatac 35400 caagcccttg cagcagcgtt ataggctctt ggctgataac cagaccaagg cttaccatca 35460 ccccaatgat acgttgtcca agtgtcaggc ttaccatatt tgtagttatc ccaacggttc 35520 cagatatacg cagcacagcc tggccccaaa attccgacct gattgtaata tgcctcttgg 35580 ctatcataca ggaattgaat ctgatttgaa agctcatttg catatttttc tgaaacatga 35640 attgtgtaga tgaacgggta ttgatagcct gggtatggca taccgtgcca agcaccaatc 35700 tggttcgtac cttcaccata aatgttagag aacggaataa cgccgggact gtacgccagt 35760 gagtcatgac gataatcaat gattgtgcaa tcaccaacaa cagccgtgaa cgcctcactt 35820 ccgttcaatg cagcacggaa caggactgtc caaccgtcat caccttcaaa cagtggtgga 35880 acatcgttga tgcagtagta agagaattct ttgtcagttg ctgtatcatc ctccagagag 35940 aagttcaact gttcaacttt ggtgtattta acctcagttg gtaaaggctc ttctgctgtg 36000 tggttaggtt gtctctcagc aagtgtaagg tttttcgggt caagagttct tttcacccac 36060 ttaccataag tctcaccaag aatccagttc cagttccact cgttgtcatc agtcatgcgt 36120 acagtgattg gtgaatcagc acggtagacg atagactttg tatcaacttt accttcttct 36180 gtcaaccaga atccgataat gaaaccgtca tcagatgttg gtagttttgc cttgataatg 36240 ttcgctgaac gaccatcgaa gacattctct tcgtagactt cttcccaagt acagtttccg 36300 tagtcggttg ctgctcttga gtcggctacc agataatcct caccagtttc atcattagtc 36360 tctttcgcaa gctgactaag aggaatgtca tagactttcg gagtagaatc actcgtcttt 36420 ggaagagtta ccttgtacct gttttcagag tctgcgtcac ctttcgttga accgattgtc 36480 aggattccag acataccaac agtctttcca gtttctgtct gaccaccata agtcaatctc 36540 aacttagact tgtcagtaac acggaagata accgcttgct gttctaagaa ggtctgacag 36600 gcttggtcag tgttgattac aatgtaacct tcagagtcac ggctgtagtt gatagttgta 36660 ctcgcagggt aagagaaccc gtagctgatt gcatccgtga atggagtttc agcagacata 36720 ctttgacgga agaatttatc cgttgagtca atctcagtat attcgttcgc agtgaagaat 36780 acactctcta aagcagtctt gtacttctca tcaccagtaa ttctgtacag aagataacaa 36840 gcatctacaa accagacttc agcatcagca gcgttaccta actggttgat accacccaag 36900 aagggggtat gtacaggtcg gttatgccac ggttcatttc tcttgaactc atacccacca 36960 aaatcaacag ggactttaga agcataattc actaagtata caccgttgat agtctcatct 37020 tcgagtttaa tcatcccttt gtcggagtct ggaagatttt cttcgataat gtcaccatcc 37080 cacaactggt cattcgggcc tttccccata ccaattttat tgtttgtcca tacgacaagc 37140 cattgaactg gaagggtttt tgctcgattt gactcgcccc acttaacagt gtaagtgtcg 37200 ccaagatagg ctttccaatc aatccacgca agggatgacc aaggttctgc gataccttca 37260 ccagttttgt gacttacacc gatgtagtca atctcgaaga agttcttctc atactcttca 37320 gcggtcaatg gtgcgccagt gtcaatacgc attgtacgga tattgacgtt ccaaccagta 37380 gtcacagcaa tctcagcaac accggatgag tcaaacttag gttcagggat agagaactta 37440 cgggtttcat cccaacctct gccaccagta ggaacccacc atacagcacc agcccaaaca 37500 ggggacccg tcttcttaat tctcagacga actgtctcat actgaccacc ttcaaaagag 37560 aggccgtctt tacgaatacc aacagggttt gtgtcatcaa cacgtggagg tttaatccag 37620 ccacctgtaa cagtcgggat aactgacgtg cttgtaaaac ctgtccaagt gttgcgtcca 37680 tctgtgaagt tgtctaagaa caagtcttca taggttggag ttccaaggat tctgtactgg 37740 tcaaaaacag tttgccaatc aatctttccg tcaaggttct cttgaatctc ctgaacagaa 37800 ccattaatcg catcccatgt catgtgacca cgatgagcaa atgtgcaaga gtcgaggtat 37860 tcaccccaaa aaggagcacc atgaggaatc tgcccaatac cgttagtgaa ctggataggg 37920 acacatttat agccaccctg agtaggttct tcggggttta ctggataatc cgcaaggaca 37980 ggctctttac cattaataat ccagttacag atgtaacgag atggaaaaatc tggtacaggc 38040 tgacccttgt aaaaatagtt gatataggca tcccaagcct tttttgcttt gtcaaggaat 38100 acaggattct ttgtagcaat gtatgcgtgg cagtaaccga ggacatgtag tgcttgccct 38160 tcagttgttg cgtcaccatt tggctgatac tctgccatag tgtctgcaat gaaatgtctg 38220 ttgtttgaca gaacgaagtc agggttttgc aggtagtggt cagtgtgtgg gtctacagtg 38280 agacccgtac tacgctttac aaaatcttca tgaccgttca gcaaattcac agcttgattg 38340 acagcactct gtgctgccgt ctgtttaact tgcgtcatta acttactcca gcggctgtca 38400 tcagacctaa ccatgactca cccaaatcgt aagaaatgaa tgtgatgatg tcggtcttac 38460 cagccttgta tgaaagggta ggtgcatatc cattagccca tttgatattg ctaggccatg 38520 tgactctgtt agaaccagaa ccctgagtaa tcagcattgt gattgtctca acagcccttt 38580 taacaaagcc tgaagagaag tctaacgcag ttgtaggatt ctgtaaagtc atctggtaat 38640 ctgtgtactg tcccatattg attgagtgac ttgcccccaac gaaaccagcg ttgaagtagc 38700 tatcaatctt cgtaacattt gggaggtttc cagcatgata aacttcatag ctgttagcac 38760 cacgtttaat ctgaacatca gagattgtta ccagagtcag tccacggttg atgttaccga 38820 gttgcagcct gttatcagcg gtgaaaccac caatagaaac tgcaacatca ttcttatcag 38880 caagagaaag aagataacca tttgcaatag tgaggttccc tttaagaacc ccaccagtaa 38940 tcgggaagaa cccttgtgcc agttggtcaa ggaacaggtt catggagatt ttcatatcct 39000 catccccaat catacctgta cgcttcttaa tcttgactaa caagaaatca gaagttgaca 39060 atgagccagt atactgcttc aagtcttgaa ttctgtaact tgtagtagcc attttagttt 39120 tccttagatt cttgcgaact cttctacaac gatacggtca acacgtccgg taacattact 39180 gtttgcagac actgaaccag acgcaccaac aacgacttca atctttgtag catccttcgg 39240 tggaagggtg tttacacagt aataagagaa tgtcaagttg gttgatgaac tgctgtcaaa 39300 caggatgttg acctgagaga caccacctgt taagtttggt gcagttggtc tggtttcaga 39360 gctttcatgg tttggctggt agcctgacaa agtaccttca cttggcttga tagttttgtt 39420 cacccatgta ccattcgtgt tgtccagcat ccaccaccac aaccaaccat cagcatcttc 39480 aatccttgcg ttaaagtcac catctgcacg gtaagcaatt gaattaaaag ctgacttctt 39540 agatgtctca ttccagaaac cgataatgaa tcctgcatcg tcatctgcaa taacagcctt 39600 gacaacgtta cctgttctgt catccagaat atttgactct tgcgcttcac taacgacaat 39660 gttaccgtag cttgaagtgt ttgcaacagc cgcagtttgg taatgaccag tgattgctga 39720 cagtgagact tcaaagtcat acttattctc accttccaca cggaatttca acattgttga 39780 taagtcgtct ggcgtgatgg tgtaagttcc agcagtatca aacacctgca tggtcacata 39840 ggctttcagc ttttcattag ggttaatgta agtcctgaag ttaccatcgt ggagaagggt 39900 ctggttgtaa cctttgattt gagcctgaat atcactgttg ctgtacagaa tcatgaaagt 39960 atctgtctga ccaatttcaa ggctgttgtt accattcaac ttaataagtc caacgcttgc 40020 agagccatca gctttctgtg cagacaagta agagttgttc ttaatagtta atgcttcctg 40080 cataactcca gagacttttg tgtagccatc tgctttaagc atgtacaggt caataccaga 40140 aagttcagaa gcagccagaa ccacgttagc attcagtgcg tgaccattaa cagttcttgt 40200 tgtaggtaca gctccaatgt ctgttgcaga tggtttattc cccgtgtggt acaggtaaaa 40260 ctgagtaccg tctgtacgca aatatgcggg gttctcaagg gacttcagga caagtggcag 40320 cttatcatca cctacaacca actggtcgtt agaattgact acagcgattc ctacagcccc 40380 tttggagcct gtcatcagac ctttgagtga tacggtgttg ttcatcgtca tgtcaccagc 40440 gaagatgtct ccacccttca acaggaactt ggacgtatca aagctgacac ctttcaaaaa 40500 attggtgact gtcattttag catcagtatc agtgcctgtg ctatctctcg ccagaatctt 40560 aacaagaaca aggtcagtac ctaaaatagt tcctgtgaat tctggcaagt cttgaagtct 40620 ataagttgcc attttacaat cctctcgcat ggtcaataaa taattatctc atgcaatctt 40680 aaaaatgcaa ataaaaagga ctccgaagag tccctatgtt aagaaaccct tttccagata 40740 taaacaactt ctgagggctg ttcgatagtc agagcctgac cagaacctgt agggtttgtt 40800 gtaccagaca ttgacaggtt gcctgatgcg ttgatgttga tgttcaagcc gtgagcatgg 40860 tcgccaacag tgttagttgc caatgaaaca tagtgggtgt gtccacccgc gacacctgta 40920 gcctcgttgg tgtagtggat ttcattagaa ccgtgtccaa cgtggtttcc gggattcccc 40980 ccaccctgac gagttgtcgt gtaggtgtgg ttgtggtctc cggcataacc agtgttacca 41040 gaagcaacgt ggttgtgagc acctgcacca gttgttgaac cagacgcacc gtgactgtgg 41100 ttagcacctg atacagtaac tgcgtggctg tgcgctggca agttggctac atcaattgtc 41160 acagatgatg aaccgtactt cgttcccgca ggacgagaag atacagcagg gtcaaaaccg 41220 actgatacac gcccctgtcc agctaaagcc caagtaccga cagggatata gttagatggg 41280 tttgtggcgt tgtcagtata gattctgtga ccaatcgggt acatgccctg aatcattttg 41340 tacaggtcgt ttacaacttt cgctgtaaca tacttgttgg ctgacgcatc agtgtacgaa 41400 agtgaaaccg tgtagttctc aacgtttccc aaaccgactt gtgctttggt tgtgttgtgc 41460 gggttgttct tgtcgttgat gtgggtctgg acaacagcat ctacagcctc tttagaataa 41520 acgttaaggt tactctgagc aatctccacg ttaggaatat cacccaagtt ctgagccttg 41580 acaagctgtt cgtcattcgt tacatttccc aaaccaacct gagcctttgt cacatttatgt 41640 gggttatgg tcaggcttgt gtgagggaac agtaaatcag ctaatgagac acgcttatca 41700 acgatacctt gcttacagtg gaaaatgtca gcatcagata atggtgtcgt tgcttgtggc 41760 aaggcgttaa tctgaatgtt actttcagcc attcattacg ctccagtatg ttcatagtaa 41820 tagatagaac ctgttgaggt tgttgaatca gttactgttt taatcaactt ccaacctctt 41880 gcaacccaat cagtacgctg cactgcactc atacggattt catgtgttgc ggtagaagca 41940 ccttttgcag ggtttacaga ctcatcaaga tacttgacat actgtgacag gttgttcaac 42000 acgtagttaa accactgtct acccatcggc tcacctctta aaaggccact cgcctgaatc 42060 tcagatggtg gtgcaacttt aagctgcgaa ccttcattat caacgtctgc tgtagcccat 42120 gagactactg catcatttagg tcttgccatt attaagttcc ttcaattttt aattgaccac 42180 tacggagttg actgacctct gcgaagtaac cgtaaatgcc acctagagcg gctggttgtt 42240 caatcttcag agttccgtct gtagtgttct tgcgaacaat aaaatcacct gttacaatct 42300 tcgaatcgtt ctcaacttcg actgcaaggt tgtattgctt cataccttca tcagccagag 42360 acccaataag gttctcttca gttgaaccag atttagttgt tacagtgatg ttttgagcac 42420 cgttagtgat gaatcggttt tcgtcttcat cggtcaggaa aagttcatct gactgaatct 42480 caacaggagt ccaagcaagg ccgttatct catcacggag aatcaccgct gaaccgatag 42540 tagccgctga gatgttattc agaattcttg cagcatcatc aacagggtca ttggtcacga 42600 ctttaaccac gattccacct gtcatcactc cagagtaatg ttcccacaat tgagaggtgt 42660 ccactgagtg tacggcttgc atagctttca tgatgctatc ggctgttgta tcagcacgac 42720 gaataagaat ctgagagtag attactaagc gatatgcttc atctgacaaa ccaacctgac 42780 gagggacatt caacaatgcc ccgatatagt caagctgaat accctcggct gtattaagcc 42840 ccttcttttt atacatatcc cacagagtat cctcaacctt ctgaagctct gtggtgactg 42900 ctgaaaggac ttgactatac acgttcttct ctttaaattg agagggtgta tagtcgtcaa 42960 gggctttggt cgtcaggctt tcaattttct cgtaagccat ttatatacct taatcaggtt 43020 cgtccatcgc agttgtagtg aactggttag tttcccaaac agtgtactgt gttgagctaa 43080 ttttcagtct ttcagaggtg taagaacctg ctgctggtgg tgtctgctgg ttgacagcaa 43140 gagctaccgt gatagactcg acaacaatac ctgtaacgtt ctcatagatg taacgcaaga 43200 tacggttagg aacaacgtca ccacctactg acagagtagc accatatttc aggatagcac 43260 tcttaatcgc atctggaagg gctgtttcct gaacagaaag agtttcctca gagtcgatgt 43320 agtaagagac tttaacgaac acgtaacgtg ggtcaggtct tgagaagtac actttgtggt 43380 cgtaaccat gtcatcgtat gctgtaccaa caactgtccc gaaagcacga ataccagcag 43440 gttttgtgtc ccaaatagct tgagccactt cattgttgtt accaccaact acgacgattt 43500 ggaaagattt agccggaaga cctgtaacgt tgtccatgac ttctgtatcg ttctcgacac 43560 cttgacatgc agaaacaccg ctcaacttct tgacagaagc aacgatagcg tcaagtgtac 43620 cgatacccgt tacagtcagt gacccgtagt atctctcacg aagctcttca tctgtctcac 43680 gaccacgccc caatgtaaag tcgtatctgt tgtaaacaga gtccagaccc gatacaggag 43740 agtcaatata aagaagagtt tcagcgaatc cagcaataga accattgtct tcagactcca 43800 cagattgcaa cacagtcgtc ttgccaaatg acatgtaggt tgttgtagta acgaccattg 43860 actgagtacg tttgttgatg tcgcctttat ctttagcgat attcatactg aggttagtcg 43920 aatccaatgt agcaacagca attagaccac tattaatcag atttgtcaac ttctgaagga 43980 caactaattt agtgtcacct accaatgcag tgtagctgta ctcgacgtta tcaatgatga 44040 taatataaga ttcaccagct ctcaggttgt tgattgatac attggtttct acacaagacg 44100 ttgtagaaat tgtaaaggtt gctttcggat aatagatagc acctgtcgta gtcctcaaag 44160 ttgttgttga tgggacttca gtacctacat caccagtgaa ctctgcaaca cctgaagttg 44220 gcatagcaat ataacgatag ataccgttca ggccaaccaa atcatcaaga ctgaaccctt 44280 cagctttgtc aattcgtgta gagtcgttaa cagcactggt tgcttcccac aggtcggcaa 44340 tttctgtaca gattgcagca agtaaaatac ccaagtcact atcttcagaa atgttgagtt 44400 tctgtgaaag gttctgaaac aggtattgac tgatattgtc aaccacctca ctgtatctca 44460 aagataccaa acctctactt gttaatccag ccattaaaatg ttaacctcgc cctctgctgt 44520 ttcaccagac aatgttgtag cagcgaactg tacagaaatc tgtctggtaa agttgtcaag 44580 tgtagaattg taagatgtca gacttgcgac accttcagtt tctaataagt attcttccat 44640 gtaattatca aagattgtct ttgcgttctt atacttggaa atttgaatgt acgggaaacc 44700 tgcatcaagg ttgaagaaaa gttccccacg tttaaggtaa catctgatgt gaatcttttg 44760 agcaacctga gtaacgttat catcttctgg aataattttg atattccctg aagcgtcaat 44820 gtcaaggtct ccatgagcga tatagatgac atcgttacca agttttctca tataaccagc 44880 accaagattt aatttgaaat ctgttgacat tactgtgcct tttgagagtt agcttcacct 44940 gaaccatctg tccagtaata gtggtgaacg tgactgttga agtctacacc tgagtcgtgc 45000 attgtaatgt tacttgcgaa catctctttt tctgctctaa tgtttgcatc agaggtgatg 45060 tctttggtaa catgcagact accttctaat ctggctgtac catcctctgc aaaagtgaaa 45120 actaattcac catactggag gcgtactttg tcagggtcgg ggttgaagtt tttagagttt 45180 gtaccgatac cgacaatagc gaaggcatct gtgatgtcgt gcgaacgtct gttatgacc 45240 ttcttcatag agccgtcacc tgccatccaa gcatccattg ggagcattga aaagattagc 45300 aagcattggt cgccagcttt aacagggaaaa gtcagtgatg caccaaccacc agaaggaaac 45360 tggataggta caccatgaat aagcgggtta ggaagctcat caacttggat gagaggtctt 45420 acagaagcgg cttgagcctt tgagtcgtag aattcgatga cagcgggtaa ggctgtgtac 45480 atctcctttt taaactcatc cagatagccg gaaataacgt tcgcaagcct tgaagtagga 45540 cttgcataag ccattaagta accttctcta agtccaactc tgttgtccag cggtcgccag 45600 tgtaatcaag attgtgagca atccctttaa cacggtaagt accgtcaaag tcaattgagt 45660 cagaaagttg aacagtgtca cccaaacgaa ttcttccgtc aagatgcaca gtgattcgga 45720 cacctgtctt caccacgatg actttaggtc ttttaccac tgaaccaccc gaagaagatg 45780 ccgatgccat tgctgcaaag tctacagaac cagaagactc agtaacctga gtgtcagatg 45840 ctgcaccgat acttgttggc gttgtagtcg ttttaagagc ctttttggtt cttctgtagt 45900 aaccctcaac agagccgata atgttacttg ccccaatagc ccaaccctca ccgcttccgg 45960 taggttcagc agggataaca cgaatctgtt tattgaaagc gaaccacttc aaaccattct 46020 cttcacaaac ccttgtcagc gcatctgaca cagaaccgta aacggtgaac ccattgtggt 46080 agattcctgt cacgtttgcc aaatcagatt caataaccgc aaggtcaaga gtcttcatca 46140 ggttcgagat gacagtcttt ttagcaactc cagcagggaa cgcaatactg gtctttgtag 46200 ttgctctttc gagtttgtca ttggaacaga ttaattttgt gattaaatct gcgcctttct 46260 tgtaagtgta actgtacaac actgtcccaa gataaacaat aggcagagag tcataatcga 46320 cttcaacaaa acctgtgtct tcatttcgtg ggaaaatgtc atcgtaccct gcacgaatca 46380 ttacagtagc accaacagct ttaaacctcg ccctcatttc tggagcaagg ttgtacattt 46440 caatcgtagt gtcatctgta ccagtttttg acttctggtc agtgtacgag atattagctg 46500 tgaattgcaa ctctgtaaag aatacaaggt tttctgtgtc tttcgcatga ccagtaggtt 46560 gattgttgaa tccaactgac tcagcaccaa cagctaactg gtagacacga aaggctgaag 46620 tatttttctc agccatttca gagccttatt gttcatcaag aggttgatag acgagaccga 46680 actctttacc aataccaaag ttgtttctgg tagggtattc cgaacttccg taaaagcgtt 46740 ctacgaagat gtctccatag atttcaggaa gtccgtaacg gcctgttaag gtggtatttg 46800 gtacacactt aacttcactg attaaaatgt taccatcagt atccttgatt gtcaaatacc 46860 aacgttcctg acgactctta aatctgagtt caagtagaaa cgggatttta tcaagggtca 46920 cagtttgtgt tgaccattcg ttatcgggta cgggaatgta atttgacatt atcttgttcc 46980 aacacctttt tcagagtttg gattaccagc atgacgtttg taatagtcag acacagccgt 47040 atcatcagtc ttcgagtaag tccctttcac accaaatgac ttattgtcaa tacagtttgc 47100 aagtaatctc ttctgagttg aattgagttt ttcaagtcca ttgtcatacg cagtcttcag 47160 accctgacaa gcacttgggc ttgaccccgt tgtggttgct gtgttgccaa cctgtttctt 47220 agctgctgta ccagccttag aagccctttt accagaagca ctgattgatg tcttaccaat 47280 gttggtcttc aagatgactt cttggaaagt tgcaacaatc ttcaaaccat tagcaataga 47340 agagtccttc ttcatactaa gtttggtgat tacacagttc tggagtcttt gcttatgagg 47400 gcaaaccagt gttaagatc tcttctggtc tctccagcgt tcagcaatcg ctgtaaaggc 47460 ttcagcattt ttgttgtagg tctgagtaag aattgtacct tcaaccgttg aaacgacaac 47520 acctgtgata gtggcaattc gaggacttct cagatagtta tctgtgattg tctcacctga 47580 ctgcataggc tgaactgtaa cgctcatcga actgtcaata tcaatatctg tgttagctgt 47640 aaggtaaagg agaagtctga ggtcatcttt aatatgaaaa tagaccccat ccttcttcgc 47700 ataacgagcc gattttacctg tagcgttatc atagataggt gtgccatctg acttataaga 47760 cgttgccgaa ttatctgtag tgatagcacc agccattatt aatcccccat gtgctcagtg 47820 ttaatgttga catcttcgtt gttgtgtgta acaacatctg tttggtattc accaatctta 47880 cgactgtcaa ggttgatatt gaacgtcata cgctggttgt tctgaaccgt ctgctgtgca 47940 gggttgaaca tacttggtac agcgaacatg ttctgtgggt tgtaaccgtt ctgaggactc 48000 agagcattgt tgccttggag ccaatcacca agtgaaccca tcatacgagt gaacgggctg 48060 tttgtgattg aatcttggtc agatgttccg agaatcttag aagggtttgt cagccagtta 48120 agtgcttgag caattgggtt tccagtacct aaagcatttg ggtaagactg ttcaacgcca 48180 gaggcatcta caccagcttt agccataagc gcccaaccaa tcggccctgc tgcctttgga 48240 gcacctttag cgaatcctaa gattcttgcc atgataccac cacctgctgc accagcccct 48300 gccgcacctg cgccagcccc tgctccggct gctgctccac ctgtagcaag ttgcttgaga 48360 ccaagaactg ctgagatgat tccagttaca ccgcgaagag ccttgacaac cacaagaact 48420 tcaccaacag caattgcaaa gtcggctgct gacttaatca gtttcttctg tccatcatcc 48480 aattcatcgt accaagctga cactctcaga agagccgcag agatatgacg aacccattca 48540 tctgcatagt caataccctt agcagcgact gtcaggactt caccaaagat accgcccatt 48600 gcttcactga ttgctgatga gtctgaaagc atgttcgcaa gagaacttgt gaatcttcca 48660 agaccacctt cagcgccatc aaaggctttt acaaggttgt tctcgatagc agcgttaaca 48720 cgaccgatac cagtaaccgc catttcagaa acacggtcaa gaccaccaac ctcatcagac 48780 atcttcttca tctgcaatga gacacgtgga agaacatctt tagcaagcag tttaccattt 48840 ttcatcaatg cgtcaagctg ctgagtgttg acacctaaag cgtcagcaaa catctgttca 48900 gcaccgggca aacggtcgcc caactgtcca cggagttctt cagcctgtac acgaccctta 48960 gaagccatct gtgtgaacgc aaccatgata cctttcaact gttcagcatc cagaccacga 49020 atacgaccaa acgttgcagc gttcttatag aagtcctgtg taccctgata accaagtgcg 49080 ttttgagcac ccgcagcgaa ccctgtgtac tgtttcatgg tctctgtgaa gccctgtcca 49140 atctgctgtg agaaaccagc agcgaacgtt ttagcatcgc cagcttgacc tttgaagacc 49200 gcttgtgcag aaagcatctg accctgacga gccacaccag catctacaac tttcttgatg 49260 ccctcaatca ccgtatatag tgaagcgaat ccaccaatcc attgtgtcag tgatgagtta 49320 gctctgtcca gaagccatgt ctgttcacga cgagccttag cctcagcatt ctcagcagaa 49380 atccaacgct tcatagcgtc gatagatgcc ttgacttgca tattatagtt tgctaagttc 49440 gttgcagggt ttttcagtgc ttcatcacga agacctgtaa tgtctgaacg aaacttacca 49500 gcaagaccct gaccacgaac acccatactt tccagtttac gtgtcagacc agaatagtag 49560 ttgtttgagt aggttctttc aattgaacgt gcagcagagt tagaagaacc tccaccacca 49620 cttccacctc tcggaggtct accccctcca gagcctccag aaccgcctcc acgcccatta 49680 ccacgaacgt taaaggcatc ctgcatctct ttcttaatac gctttgcaga ggctcttgca 49740 gccgtttcta cgcccttcac ggaagcctta ccagcattcc caaaatccat actcttagag 49800 aaagccctgt taacactgtt agcagcggtt tgagcgtttc gcacaatctt atcgaaacca 49860 gctttagact tgtttccgtc gattgatacg gctttgttga ggcggttttc aattcgggtt 49920 gctgctgcca tagcagtttt ttcaaccctt tgcaaacctt tgatgacctt ctcattgaaa 49980 ccaagctcaa ttacgaattc atcaactgtg tatttagcca ttcactacct cggagatttg 50040 tttttacttc tgacgtattc ataataggct tgttcttctt tgtaagaaga tttcacttcg 50100 aggaattctc tcaggtcaca cgcatcaacg aacgccattt ccaagagttc gctgtaggtc 50160 tctttacagt cgtcattccc ataaagatac aaggcgaact tagtcgcctc atcaggttta 50220 tacccttcat ctacagcggc ttcagtctga gggcatttta ctgctccgtc tccgactctg 50280 aaaacaggtt tttgaaaatg cttgcttcga aaaaacttga gaagttcttt tcaatagcga 50340 agattacgca atccatcatg aatccatagt taccagcaaa cgcttggtca agggtcattg 50400 gtcggtcatc aaccagcata ccatcgaaca gtttttccag aacggagtta acatccatct 50460 cgtccatagc gtccagacag ttcaagacaa tatctttaat cggaatgtct ttattaccct 50520 ctttaatctt actgaatgct tcgataagct gaccatacgc aggtgcagca acacgcgcaa 50580 tcttcagaga aatgttgaca ccatctttca ggccaagtaa ggtaatactg acttcgcggt 50640 cagcgataga ctttttaacg gttttcattg tgaaagtttc ctttatacaga aataaaaaag 50700 gagcctccat agagacccct tgaaggttat acagacgtta cgcctgaagc tgggattgat 50760 acggtgtaat caagattctc acaaccgaac gtccaagttt tactgttctg gtcagcacca 50820 agttcaacct gtggaagctc ctgaagccat gcgttacgac cagttgcaag aacagaaccc 50880 gatgggtcat agatgacgaa gttagaagag atgttttctt caagttccat caactgctgt 50940 tctgcataga ttgctgcaag acgtaaattc gaagtcgctg tctgcatcag ttcaatttca 51000 attgtacctg ttttatcagc gtttcgtgtc agggctacgt caccacctgc acctacaact 51060 ggagtaacca gtgcagatgt tctttgcagt cgaaggaaag aatcaggggc gaaccccgtg 51120 attgcaatac cgttccagct acaaataaca tccttcggtg aatactgttg gaaaagagac 51180 attatatatt accaccatta ttcataagag agagtaccct gcaagtttac agtcaggatt 51240 gcaccagcga tgataccagt gaatttaaca ccctgaagca gacgagcacg tttgtcgcca 51300 acggaaacgt cagcagcttt tggcatagta actgtgaagc tagacaagaa ctcacggtta 51360 accgctctct gaagtgatga ttcaataacc tgacgaacac gtgtgatacc acggtcatca 51420 taagtaatct taccaccctt ctgattgata agcaagtctc tcagagaaga tttcaagtca 51480 gactcaagcc agtctgtacc acggatgatg tcaatccact caccaccaac tgtcttaccg 51540 ctacgcatca cactgttacc accttcgtag tcaacatagt tcatgttacg tgcttccaga 51600 gccgctttct gagttgagtt cagaggacgg ttgttggtag gctgacgtga atagtcaata 51660 cccgtcaaga ctgcgttacc ccatgcgata gaacctgcat catacggagc accgtaagcg 51720 atataggcca tttcagggta ctggctttct gcatcatcat gccacacaag gactgtacga 51780 gtcatcttgt tagtagcaag ataagcacca atatcagttg ctgaactgat gttcgttcca 51840 gataatgacg ccgcagagtc agtagctgtg aagaaaatct tagtacgaga ctgaatagct 51900 gctgccattg cctttacaaa tgtttcgtta cgttcatctg ttgcaatgaa gtaccaatca 51960 tttgtataat cctcaatacg cttcactgcc tgttcagcag agtcagaagc tgtagaagag 52020 ataacaatgt tagttccaga agttgtgaca cgtgcaaagt ctacatcagc atcttttgca 52080 gtaatcagca acgtggcgtt agcaccagta cctgacagag ttgtagtcac agttggtgaa 52140 acagttgtgt ccgtatcaat gcttgtcttc agagactgta gaatcgtctg agcggtactg 52200 ttagtagcac cagtagcaga gtatgaacca actacaccgt tgtttgtagt cacagttaac 52260 aggtagttat caccagcaga cccgatagta gggattgcaa gagaatactg caagttacga 52320 cgaccaatgt acagttgagt tacctttgga gtttgcgccc aaagagcctg tgcagcaccg 52380 tatactgctg agtctgttgt gaagtcttca gagagttcac tcagagaggt atagccacga 52440 acacgctctt caaacgcatc atgtcccgca aggaacaatg gaagaccgaa accttcacgt 52500 gtagagccag ttgtgttcag tgtaatatcc acgtttacaa ttggagtgta agccattttg 52560 ttactcccga taatcttggg tatgttcgtc taaatggata gctacagctt gggtatcacc 52620 ttcaccataa tgaagaatac cgtctgcaat aactctttca atgacactgg aatcatagtc 52680 aaccagaatc gaatccacag ctaattcaag aatcagcgga gtagacggct catagtctgt 52740 ttgaagcatg tcaaagtttt gcggcatctg tccagagtct acgaggattg ctccaccagt 52800 ataatctgta agacgtgcgc gaagtgaaga gatttcaagg cgttgcttga attctgaaat 52860 gatgccaaaa gagttctttc cagttgctgt aactcttatt tgcactttga aaggaatacg 52920 atagcacgtt cttccctcat cgtcaacaaa aacatcgagt aagaatccat aaggtgtcgt 52980 taccccaact gaacgaatgg aaatgaacgg catatcaggt ttcaaaccct tgtcatttga 53040 gttatccgat ggattctcaa taatgacgaa aggtctcttg ttcttgtctt tagcaaggag 53100 ttgaccaaca gactctaaag tgtatttcac aacaccatct tcaatacttt caagatttaa 53160 tagagacatt attcactcgc attacggact gtgttaatct tatctcttcg gatgaaaagg 53220 tattcgtaat gggatgttct ggctaactgc atcgaccaat ccatcacact gaaacattcg 53280 tattcaacgt tgtccagtaa gaggatgtca gattcagtcc aagagacttc atcagaagtt 53340 ctcagcttgg caacagtgta gaggattctc acatcagttg ctacaacacc ttttggaaga 53400 accttttgag tcagactgtt attgtatggc tgtaaattac cacgaatagg gacagttact 53460 gaattctctc cagataccca acgaccgctc tcgttgtagt acccttcaga ggtttttctc 53520 acaatctgaa gagtgtgtct tcttaatagc ctcatttcct caatcctcca cgattagaag 53580 tcttgtacgc cagtttatca acgaggtcag atgtatcaac cagaggcgta tttcttccac 53640 ccttcgattt aatagttgaa ggtgtgttcg gtggtaacag gcttggatta ccaaagacct 53700 ttttaaggga cttgatagtg ccttgtccga attgggaaag tacgaggtcg gggtttgtct 53760 gccctgttga gagcatacgt tttagattct tctgagcttg cgctatcaaa agttttctgt 53820 caatctgcat agcggtttca aacgccctgc ggtagacttt accgttggct gctggaatac 53880 cgtttacttc atgaagatac attaacgcag gataagagaa acctgagtag tgcttacctt 53940 gttcagcaaa aaagccaacc tttacagttg actttgcaat gtttttgacg gctttcatta 54000 ggacgtttcg cccctcaccg ataacacgtt ttaccattat gattccttat ctacgaaagc 54060 aataaccata cggttaggac tcccgttaaa atcaccttgg caacggttgt catgatagct 54120 gaaagcatca ccgtactcaa cactcttgtt cacagaatcg ggattacatt ttctcaattc 54180 aatgtcacat ttgctgatac cacccgcata aggtgttcca acaataccat tcttaatcaa 54240 gtcgtcaagg gcagatttaa agttggcgaa gcggtctgag ttgcgctcat agaccccacc 54300 aaccttctca tcagccatct tagcaaactg cattaagatg tagcgaagtg cctgaatagc 54360 cgctctacgt tcgttcttat cgttcgaaac atagaagaat tcaagcacag aatcatcaac 54420 catgaggtca tcattatcgg tgtctgtgca aagaattctg attctgtcaa gagggttagt 54480 gcttgggttt cccgtataca tttaaccctc ctgaagagtc ttatgaagcc ttgtcagcgc 54540 gaacgtcaac cagcaactga ggacgagtac agattggcat catgtaagaa tgagcctcga 54600 agtcagtacc ttcgtcacgg tctttcgcgt attcaaacac gtacaagtcc tgaccaagag 54660 tattcacata acccatcttg ttagcagggc cataagaaat ctggtacagg tcgttcgctt 54720 cacccaacat cgcagtgtta gggaacgcat gaccaacacc tacagagtcc tcaacgccgt 54780 tgataccaac cagcttgtga acagtgttac gcttgtcagt gaacttaccg ttgtactgac 54840 ggaacaccag accacgataa gggaactcgt tagtcgctgt agcaacacca tctgaacgac 54900 cagtcttcag agaaccagta atcagaccgt aagaggttac tgcctgttga gccatgtacg 54960 cttcacgtac tttagcatga ccagtcagct tacggaagaa cgcacggtca accagtacga 55020 taatctgttc gccgttagtc agaccaccag tgttcgcggt gtcttccatg tgctcaacca 55080 gttcgtcaac agcaccgtcg atgtctgcat caggattgtc aaggtcgaag tacacagtct 55140 tcttctcaac gccgaaggtc tggtacatgt cagcccaaag gaaaccttta gaatcgacaa 55200 ctttaccttt cagagcttgc ataagcagga actctttagt aatgtcgtga gtcatacgaa 55260 tcttctggag cttacgagca cgaaccatag cttcagtagt cagttcagca gcagtaccag 55320 cctgacgaat accctgcacc tgttcaggtg tgattgactc aatgtgcttg aagtaaatca 55380 gtgggaacgc aacctgacga agagattcag gagcacttga ctcagcgtta cgagtatcgc 55440 ggtcaacagg gtcaagcaag ctgataccat aatcagtgat gtccaacagt acagaggtct 55500 gtgaagttgg gactgaacgg aacaggtcaa gctggttgac ataaccgtaa gtgttaggga 55560 tgttagcaat atcgcctgtc aggtcagcca acaggaaacg tgagccattt gcaagagtta 55620 aagacattta gatttccttc taaaaagaat tatttaccag ttggaacaag gatgatacct 55680 ttagctttca gggcagttgc cacagcagct tcggtcatac cagccagatt gattccacct 55740 gctttcagtt ctgcttcacg aacaatcatg gtgatgttca cctgacctga agtagtcaga 55800 ccaacagcag ctttgaagtt gttcaggcag acaccgtaga tgtcagcggc agcggtagct 55860 ttagtaccat cagcctttaa caggtcgcca atttcgtaat caagaccagc agcaggggct 55920 acagattcac ggctaaaacc gtttgaagaa atctgttccc acagtacgat gtccatcagc 55980 ttttcacgtg tacctgtttt aatgtagcca gcgtaagcca ttattgtatt cctcagtttt 56040 tgttaagttg agcaatcgca tctgcgagag cctttgagcg gagttcagcc tttgactgag 56100 cttcagcttc ttcagcgttc tgttcaggtt cctgaacggg agcttcacca ccctgaccag 56160 cttcctgcat agatgcgctt gtttccaatg cttccagtgc tttagcgtgg gtctgttcaa 56220 cttccgcttc aagagttgca atacgctctg aggcttcagc ctgaacgctt tcaatcttgg 56280 cagacgctgt gtggataaca gaagtcatga atttagcagc atcactttca gacccaagag 56340 ccttaattgt gtctacagcc atctgagcat caataccgat tgcttcagca tgaggcttca 56400 tggtgtcaag cagttttgca tctgcgccct gattaatctg agcctgaagc agcgcaatct 56460 gttcctgata agccttttct ttttcggcat ccatttcaag ttcctcggag ttagcctgtt 56520 gaatcgctgg ttgatttgga acattaacat ttgcattggc tccaccaaag atgaaatctt 56580 tgaattcgtc agtgctttta atgccgtcaa tcagcttgag tgaaagagcc tcgtcagccc 56640 cataaactct tgcaccagta tctttaacag cttccgcaga gataccgcga gaacttgata 56700 caaggttgac aaaagatgtg taagaattat cgaccgactt ctgaatcaag tctttagctt 56760 ggtcagaaag tggttcatac gggtttccca ttcctttaac ttcaccagct ttgatgatgt 56820 tggtttggat tcccgctttc tccattgctt tagagaactc ttgatggata acaatcacac 56880 caattgaacc tacatcagca tttggagagg catagatttc acttgcagaa caagccagcg 56940 cataagcggc tgaacaggct ctttcatcga cataagcgat aacgggttta ccgttagagt 57000 gttctttcat gtgtcttgca agttcgaaac aaccagcagc ttcaccacca cctgaatcaa 57060 tcatcagaac aatcttttca actgatgcgt cagcaacagc ttcatcaaat gctctggtca 57120 atccctgata agatgacaga ccaccagtac acattgcatc aatgccagac atacgatgag 57180 agagcgcccc gaatacattc aggaatgcta caccgtcttg gacagttgat acatagtttt 57240 ctgcttgacc tgttacaggt ttgatattga cagcagccat agggtcaatt gcacctgcca 57300 tacgctgatt aatatacagc ataacagatg cagccaaatc ctcagtggct aacaggggtt 57360 tattttgaat ttgagccgct agtctgaata gttgatgtga cattcagacc tcatgtgttc 57420 aaatcaattg agatagcgtt aagagtgtac acaccagcct gaacgtagaa ctcaggagtg 57480 tttaactcac catttaaaac aagtctatca cttgaatccc agattgtaaa gtgggaaatc 57540 gtagaatttg caggaatttg aaaaataact gaatcctgtg atgcaaccag accgttttgt 57600 ggagttccaa agacgattgc cattttcgaa gtgaccatat ttgctgtagg gtcaggcgaa 57660 gggtctgagg aatgaagagc aactttcatc tgagatggta gtgcgctcaa catgagattt 57720 ttcgtgaaag tggttaatgc cattaatcca accttaaaag ttgcttggaa acgattgagt 57780 tgttcccttg gctatccact acagataaca ctgtatcata aatctgacct ttgacaagca 57840 ctttatagtc acttttcaaa aataagtaag acactgttcc atctgagtta atagatgcgg 57900 ggtgtgataa ctcaccaatt gcgatgtatg cagcggatag ctctgttgca tccaactttt 57960 tgccttgaca atcatagagt tggtaagaca gcttcatagc actcccaaaa gggaaactga 58020 taactggctg ataagaacct ctgataaagt cctctccagc cagtgaaaca tcagtatgtc 58080 tgcataaagt aatctgtcca ctcttaccat cagtctgacc ggagccagag attttaactc 58140 cctgactcac agtcaacgta ccggattcgg atgattggtc ttggcgataa tctgacacac 58200 gaactttgta gctgagtgtc aaggtatctt ctgccattac ttaccctttt cgctgaacgt 58260 tttcgtcacg tcctgatgga gacttagcag tcccttcacc tgatgttttg tacccttcac 58320 cagcttttga ttggctattc ggtgacagct tttcagatac tggtgcagtc tcatcggctt 58380 caggaagacc aagcagacta cgcagatagt tagacatgtt cttgtcaact tccattgcac 58440 caacagaaat acaacgttgg atgaatgctg cgatgttttc aaggtcaggc ttctcaatgt 58500 cttcaaacac gattctgaca tgctcttcat cgtcccactg gttcagtgag taagtctgag 58560 gaatcaggtc tttgttgatg acgttttgaa tctgacgaag aatggtttca acggacattg 58620 caagaaggct cgtcttagag tctgcgagac tgtaagagcc atacttactc tgacccattg 58680 ccaatacgtc tgacatgaat gacatcataa tctgtttaga atatctgtca atgatttcat 58740 cggtattgta agacttcgca ccctgcttac tcaccagctt gaactcgaac atgtccatct 58800 ttgactcagg gtcaatcata cgtgggaaca gaagtccagc acggttgttt gcgttgatgt 58860 cattgatgat gttctgacag taagcggcaa aggctttctt ttcagggtct gcgtcacggt 58920 caagatattc tggtgggagg ccaatcatag gcataccgac caaatctcga ctcacgccca 58980 ttgcttcaaa ctcttcaatc tgaaccttgt acttccaagg aacgtaagca ttcagaagtg 59040 gtgagcgacc ttcagggttg ccaacctcat catcgtaacg gaagagcatg aactttgaac 59100 gtgggagttc gataaagttc ttaacagttg aacctgccag atgagggata agacccaagt 59160 tctgacgcgc acctgtcaac tcacgattat tctcatcgaa gtaccacttg tcaatcgtgg 59220 tttggcttct tacagggagt ttagcccatc caatgagttt atcattgtaa cgtgaagggt 59280 atttcccttt cttaccttta cggactttgt agaccttctc atgaagactg aatccgtaaa 59340 cgcacataga catgattgag ttgataaaat ctccccattc aacatccata tcacctttca 59400 gtgtttcgaa gaacttgata cgctcttcat aatcttttgc atcgaatccg tctggagcat 59460 caaatctcca gttgactctt ctgaccaaag tcttcaggat attgatagac gctgcaatag 59520 cagggtcacg catcataatc tgaaaggtct tgatgctctc agggaatctc aagtttctac 59580 gaggctcttc aaagagtctc ccgctgatga ctttgagacc aagagaaccc atctcgtcct 59640 tctgaaaagc tggcagttca gggttaattt cgttctgagc cattgtcata tttcctaaat 59700 ggttttgata cctgcgaacg gattatctct cagcatatca agagagttat ccattgatgg 59760 gacttttaat acaccacgac tgttcagact ctggaaagca tcacttgtgg catccacttg 59820 gtcatcctta cgtttaccgt caccagtgaa accttcgagt tcagtgaaat aggcatcgtt 59880 ccaatcgcct ctcacaactt tgacatgtcc tgattcagca acagcagaga atggggcaaa 59940 gcgtgtaacc ttgtccttat ttgttgttct tgctcttgca cggaatccgg ctaatgccag 60000 ttttctgatt aaacctgaag cgtaggattt accagcagcg cctgggtctt gaggaataaa 60060 gatttctacc ttagacgtat cagccttggc agtctcttga ataccctctt caacaccagc 60120 gggacggtct ctgaatcgct taacatgctc gatgtagaat gttccggtct tctgaccttt 60180 ggacatttta acacctgctg tccagtcagg gtctggattc aattctgatg ggagtgttgc 60240 agctaagtcc catgctctca cacgtgaggc tggttcgtca actgaatcta cgatttcaca 60300 ccatcttcta tcaaaatagc ttgaaccttc agcccttgct ttccagttac cataacgaag 60360 tcttgcaacg ttgataggtg tattgttttc aagtctacca cgatatttcg gttcgataaa 60420 gtccagaatt ggattatcat caattgtacc agaaataaag gtatatgtct gtggaatttc 60480 taatgggaat tgacgaagaa taacatctct gtcccaatca gaatacatga caccttcctg 60540 aataacaaac cagcgagtct taccacaaag ttcattatta ggatagccct cttcatcaag 60600 gaatggctct acccaagcat atacgaagtg gtctctttcg gggttcatcg aacatttcat 60660 gtacgagtca ccatctgcac cagaacgcat acgagtcatc aggtaatcaa cctgactctg 60720 tgagaagtga gtaaactcat cccaatagat tgctgagtat tcgataccct gatgggcttc 60780 tgcgtgtttt tccagttcca tgtaagagaa cttgattgat gcaccagaag ggaattgaat 60840 caccatcttc tgttcatgtg gaactccccc gaacatacca tacaactttt tagcggcagg 60900 ccaaagacca cctgatagct gtgtggtgtt tttacggaag aatacggcgt agtaatcagg 60960 gtcatcttta tgctgaagag catccatcag gatagcaacc gtcttaccgg aaccagccgc 61020 accaccatag agtgtgatgt cagttgtggt gttcagcaat gcctcttgag agccgggctg 61080 tggggcaata aacttcttat ttgtcaacag cctcatgaga agttcaacct cttcaagcgt 61140 atactttctc aatgtcaaga tttgacttgg aagcagtttt tcaactggct ttccatactt 61200 ggcgagaagt tttacaactg ctggctcaag tttgatagta cccgctaaaa cggcctgcac 61260 gtccctcaga cgctgctgtt taacttcggt aaggttcatt agggttactc cataaaatag 61320 ctcacagagg cttacacagc ttctgagacc ttatccaata gctcttgcag gttatactca 61380 ttcgggtcaa ccttcagtga ggttgcaagg ctgataatta cgtttctgat aaccgcactc 61440 atctcctgat tggattttga gaggtcatac agttcggctt taagttgcag ttctttaatc 61500 tgttgttcgt tcattctttg ctccataaac gaaaaaagga ggctaaaaag cctccgaaga 61560 aatgagttga aaatgtacac actactgaca gtttggaaaa aggtggcgac tagcgccaca 61620 aaggaaaccg caactgcctg tatgccgaaa catactcaaa aactcttcgg aaagacactt 61680 taagtatgac tcgaaagctg cctcccaaga gccaagaggt agcgataccc tgactctcaa 61740 gaggacttat tggagacgcg agagggaatc ccaccctcat ttttccgttt tgcagacgga 61800 caccattgt gtttcagtca tcgcgccatt atttcgctgc tgctttcaat tcatcggttg 61860 ccacgttat tgtcacggca ctcttacaag agtccttgaa ccaatctggc atgtacttcg 61920 ccataaacgc actggcttga gtcttcaggt aatcactgat taggcttgct tcttcagcaa 61980 tcatatcaac aaccttctgt gcaaactctt tggcctttgt gataagctca ttaatctttg 62040 cgtatgctgc tgctgctaag tcctgaatag ttgcaagacc ttttgcgata gcatcattca 62100 acgcactgat agctgagtta actccggtca tcaccgtgtt gtaagcattc atcgctgact 62160 gtccgattcc ttgtgcaaca cccattacgg agttgaaagg tgaacaacct gcggaagtgc 62220 ctgtaagaga ttcataagaa ctcttgtaac cctctgccaa ctgcattctc tgagagaact 62280 cgttaactga ggtctgacca taactgacga gacttgtaac agtcgaagct gaagaggtta 62340 aagcccctgc accacttgtc agtaatgtcg tgttaatgcc agcattctgt aaagccgtct 62400 gagcggttgg atttgtcagg ttgctcttca gtgtgttcat tccagatgta ccggaactaa 62460 tacttgaaga cagacttgat gatgcactcg ccagaggatt tttaaaagcc ttaccagact 62520 gcattaaact gaagatttgc ttagtttggt ctgtgacagc cattatacac cttacgaatt 62580 ggaggaagat accagacttg aactggtacg ccgatttctc ggttactgag tgtttagcaa 62640 acacttccct taccatttag ggttaatctt ccagaatttg gggagaagga cgggattcga 62700 acccgcatag acctgattca cagtcagggt cattacccat ttatgatacc aactccattg 62760 gtaggtgatg agagattcga actctcaaca tcggaggttt aagctcctac cctctgccaa 62820 ttggggtaat cacctattgt ttggcaggac gtgatggatt cgaaccatcg taaccggatt 62880 cagagtccgg tgtaataggc caactatacg aacatcctat tggaagacct ttcgggattc 62940 gaacccgaac ctctcaagtt cgtagcttga gtctctgtcc atttaagata aaggtctatt 63000 gtttggtagc tgtgggaggga ttcgaaccca cactgaatac gttcttagcg tagtgcctct 63060 gccaagttgg gctacacagc cattgtttgg tactcgcaag gcgactcgaa cgcctgacca 63120 gcaacgtgta aagatgccgc tctaccatct gagctatacg agtattgttt ggtgctccca 63180 cacggactcg aaccgcgatg acctgattac aaaacaggca taatagcctt tatatgatgg 63240 aagcattctg aattggcaag gggtgaagga ttcgaacctt caacttacgg gtttggaagc 63300 cgatattttg ccagttaaac taacccccta taatttggag caagtagcgg gactcgaacc 63360 cgcaagagcg acttggaagg acgccatgtt accaattgac atcatacttg catttgtcaa 63420 gcgactcatc acacttctca gtgttattaa cgctctactg gttatcctga cagtcaggag 63480 atacagacca cctccttaat tgccccgtca tttcagggac gattataaag acatctgaat 63540 ctggtagcgt aagtgggagt cgaacccaca ctgaacaggg tttgaacctg cttcctctgc 63600 caattgggat attacgccat tcaatctgaa agactctcgt aagaaccttt tgggttgaag 63660 ccccgtttag taatcataga cgggacaact ctacgccgaa ttcggtgata gggagtcgga 63720 agaccctact ggtgtttagc tattacgcca ctgccagaaa ttgagcatca tttgcattta 63780 ttttaaatga taaaaataga agcataccca acgaaaacta cggtaaagat tacaggagat 63840 tgtttcacct gtcaacctaa atttggcgag tgattaagga ttcgaacctc tccacgacct 63900 attgtcgccc cttactcata atctgttagt ccggcttcac aaactacttt caagaactat 63960 cgtcagtaga tactgcattc ccattttacg gcttcaaagc tggcctagct ccaccgttgc 64020 agaatcaccc atttttggca caccctacag gattcgaacc tgtaactaac tgtttagaag 64080 acagtggaac tatccgtttg ttctaagggt gcatcatttg gcggggggcat tcggattcga 64140 accgaaaact gacggcctca aaaaccgtag agttgccaat tactccatac cccgattgtt 64200 tggtcgtagg tgagggtatc gaaccctctt ctgacggtta tcagccgtct gcattaccat 64260 ttatgctaac ccacaattaa atctcttaga agtggtaatc ggacttgaac cgatgtaatc 64320 ttccgtatga aggaagtgca tgaccatctc tgccatacca ctactaagaa atttggtaga 64380 ctatcaggga gtcgaaccct gaacctccccg attaagagtc gggtactctg accagttgag 64440 ttaatagtcc atttgtttgg tcggtgtgac aggcatcgaa cctgcaaccc tgtggttaaa 64500 agccacccac tctgccagtt gagttacaca ccgttgaatt tggcggtcta tcgggattcg 64560 aaccctgttc cacggctttt cagaccgttg ctttaccata taagctaatt agaccgtggc 64620 ggaaggagag ggagtcgaac cctcaaggcg ctattaacgc tcggcggttt tcaagaccgt 64680 tttcgtcgcc agtcgatttg cccttccatt ttgtctctct gtcgccctga gcgagactac 64740 tcagttatgt agcccaaacc ttaaacgcaa ggaggtgaaa cgtttaagta aaagctgtag 64800 acctgtatat ttcatctaaa tgtgctttgg aaaggagaca tccattcacc tcataagagg 64860 aacgctacaa agtagaatca ccgcatatac cagacggcag ttaccagttg gacggtagtc 64920 tttccggtga atttggcgag gatgaaggga ttcgaaccct catattccgc atagacagtg 64980 cggtacaatt accaagtctg ccacatcccc aactttaagg agtctttgat tcgacgctct 65040 aaagggtaat accctacacg tcactcagaa caacccaaac tgagtaattt ggatgcgaag 65100 ggcagattcg aactgcctac accggataat gaaaccagta tgatgccatt tcactacttc 65160 gcaattattt ggtgcgccta actggactcg aaccagtaac ctttcggggc ggttttacag 65220 accgctgcat tgaccatctc tgccatagac gcataaattg gcgggaaagg tgggactcga 65280 acccactacc atctggttaa cagccagccg cacatccata tgtgcttcaa accctttaaa 65340 tttggaagcg ggagtaggaa ttgaacctac gacctccaga taatgaaact ggcaagctac 65400 cactgctcca tcccgctata aaactttaat ggctctggac aaactaaccg tttcggcaag 65460 gtcattccaa tttcgcattc ttcagcacgt gacttagaag atgctatccc aaagctctgc 65520 aagcaaccgc gaatccttac agagagttta tcagtcaaga ttctaaggga gaatttgacc 65580 agaccattaa agttttggtg gatacaggag ggacttgaac cctcatcctt ctcattgcaa 65640 gtgagatgct ttcccaatta agctactgac ccatactgac agaattcaaa gtatctgtca 65700 aactttattt tggagtcgta gacgggattc gaacccgcga gcaactgatt gaaggtcagg 65760 tggctaacca tttgccttct acgacattaa tttggtctcc gttgaaggat ttgaaccttc 65820 gacccgaccg ccccaaacgg cctactctat ccatactgag ctaaacggag atgttttcta 65880 aaactatgaa gctatcttat cgcacttatt ggagcctgtc aacaagattt ttaatttatt 65940 tttctaagag taggcgcgtt ctgtcgcctc gtgttattta ctcctgattt atcatcaggc 66000 taccttcaga gcagcaggtg aagggactcg aaccctcatc cacatgggca aaaccatgta 66060 tgttccccat ttacaccaca cccgcacaga tttctgtgaa acctctgaag ctctctcaga 66120 acagatacca aggtaacatt caacttcgta tctgtcaaca actattttga accaattctt 66180 gcaacattct ggaacgttgg agcttcctct ttctgttccg gtacatcatc ttcttttttc 66240 ttgtcagatg caagtgcttt catctctgaa gaagactgag taagaataat tttcgctgct 66300 tccatcattg ctttgacatt tacgtcagat ggtgaaagcc cgaactctgc aagttgtgct 66360 acatctctcg ttacaagagc gcggaacaat tttacagaca tctcacttga gtcgtcaagc 66420 agtaattgag ccttgacaag agaagattta tttctgcttc ctcgcggtct tccagcagga 66480 ttgccagatt gccccttgcg gaactgacca gcatgttttc tagccattat taaatcctca 66540 atcaaacatt atgtcaagta ataattttaa ccttaatagg tgttttgtca aggtgtttct 66600 tgccctcgcg tgtgcttgtg ggcgtgagtg cgtctttaca gttatcttta aagactttta 66660 agagataatt agagatagat agataacaga tagaatataa gaagaaatct ttaaaaagaa 66720 cctgttcaga tgacagaacg gagtgtagca cacaaaaaga gacctgtaaa gtggagaatc 66780 accottatatt tgaggttttt cgtcataaat gagtaaaaat cgggttaaaa tgatgatttt 66840 catgactttt tcttcaattt tatgcaacaa attgcttgac tttaccccca tattatgaga 66900 atatactcca tagctgtagg gcgaggcatt attaggattt actaatgact caaccaattt 66960 taaacaaacc tgtcagaaag aaagcgaaag ataatcaaaa gtctccccaa aagaccaaga 67020 gccatctttc agagcactat tctgacgacg aattagcatt ataccgtttc tttcaaggtt 67080 atggtaagat tatgtaggtg gtcgtaatgt ccagtattct attaaacagt tcagaaatcc 67140 aagaggtcta cgaagattat cttctctatc gtttggcctt tggtggaatg cactctgaat 67200 acgcaggtgt tatgactgac ctgaataatc ggtcaagaaa ctctgaagag atttttatct 67260 ctgactatga gaatcgaatc ttgatgaaaa ttctttacct actgaataga aggaatttga 67320 aatgactgtt caaagaacat tcaaccccga tgagtcagtt accctttcgg gtactactgc 67380 acttgagttg attggtgaaa tgtacaatca gattggtcag ggctttaaat atgtccctaa 67440 cactttcgtt gcaaagacac ctttcaatcc aaactttaaa gtcactgtta aacaggacat 67500 tgaagaggtt tctcctgatg taattgttca tcggactgac cttaaagatg gtgattcaat 67560 tatccattca ccgaatgctg agtctctttt aagaacactg agcaatgctc tgattaagca 67620 accaactggt gcaactgtga agtctgcaac tatgactctc ggtgcttaca gtgctacggt 67680 gagcggtctg tctgctgaag tctctgaaca gattcctgag acagtgaatg aaccagtaca 67740 ggacgacatc gaacctctgt ttaagacgaa cgatgaagac actgtgaaat cttctgaacc 67800 ttttgatatg agcattgcaa tgtctaaaga gtctaaggaa gagcttgtag agtatgttga 67860 aaagtatggc atcactcttg acaagcgccg taaccttgca gcaatgcaaa accaactcca 67920 aacattgtg aacgaaggat aagaaatggc tagagaaacc tttaacgagt tcgtggcagc 67980 ggttatcgct aaagcgaaag cagacggtaa aatcactgac ttcagtaaag ttcagagtta 68040 ttacttccca cgctatttct actcaggtag ctggaaccgt ctgatggatg tcgctacaga 68100 gatgaccgga acatatacac cgaagggtgc tgtaccgttc tctgcaacca atatgtacag 68160 tgcttcgcgt gttgttcgtc tggttgactt cctgcatgat attcagtatc tgtccctgaa 68220 cggatatgag attgaacaaa ttctcaaccc gactgataaa gtcaaaccgt tcttctcgca 68280 gcttaatgct gttatcaaag cgacgactgt tagcgtcact ggtgtagcaa ttgcaccgaa 68340 aactaacaac cttgctgtag gtggtactcg tcagttgact gcaacggtct ctcctgacaa 68400 tgcaacaaac agcaaagtta cttacaagtc ttctgacgag tctgtagcaa ccgttgatgc 68460 taacggtctg gtgactgcaa aagctgaagg tacagcgact attacggtaa caaccgaaga 68520 tggtgcgaag accgatacag cgactgtgac agttgcagca gcataactta tagggactct 68580 tcggagtcct ttttatattt agtgaggtaa tcatgaatgg aagtttctca aaaaggtcag 68640 caagcccttg aagtaatgga agggttcagt gcaaaagcat atctggatgt tgcaggtgta 68700 ccgactattg gattcggtga taccagtgtt cgtgcaagaa aagttaagat gggtgataca 68760 accactctcg aagctgctaa agccgaactt gctctggatt tgcatgactt caaatcaggt 68820 gttgaaaagt atctggtcaa ggccgttaaa ggtacaacac agaatcagtt tgacgctctt 68880 gtaatcttcg cgtataacgt gggattgact aactttgctt cttcaagcgt actgagaaat 68940 catcttgcag ggaactttga agccgctgct gcatcatttg cactgtggaa taaaatcact 69000 gtcaatggta agaaagtcgt tagcaagggt ctggtaaaca gacgtgcaaa agaagttgaa 69060 atctacttgc acagcaacta tggcgtttga tataataggg gactcttcgg agtccttttt 69120 cacatttat ggagagtaac atgaataact ttacagagtg ttctgcatca ggtgctggtt 69180 gttcctttaa agagatgaca atcccgtctg agtttatcac tggtattgaa ggtggtgaac 69240 cgaagcacgt agtgcagtgc gaatattgcg gtgcggaaaa gccggattat caggccatcg 69300 cctctggcaa atctgatatg attgaccgtt tcaacgctgc taaagaagct gctgaaaaga 69360 acggtacagt cacttttaaa aagctgtaac acctgaatca catacaagga aatagaggaa 69420 tgattctcgg aagatttctt gaaaatgtac ttgcaacact cttggtgttt gttgtagtgt 69480 atcacttcga gtggatgaac ccgtatctgg caagtggaat cacattctta ctgtgttatc 69540 tgcttgatgg tcttggaaaa ctgattggag agattttctc atgaaacatc gttacacgaa 69600 caaccaatgg aagaagcagt ttagacgtac ctttaagaat ctcttgatag gtgctggctg 69660 tgactccaaa actgctaaga aactcgctaa aggtgagatg gaagcggtca ctgatagcta 69720 cagtgcaatg tccttcaaca tgtatcgtcg tgaatggcgt ctgtatgacc ctaaagacac 69780 tgctgaagag aactggtcat attgggagga atgatgtaca agaacgctac acctgagcag 69840 tatgctgaat tgacagaatg cttgcaagag tttaaaggat taccgtacaa cgcagaaaca 69900 ttaggacgga taagagagaa agttgcttta cagtttcctg aagagattgt gagaacattc 69960 catctgaaca tgttgattaa cggtagacct gtcaccaaac tgtgattcct ctttatgtat 70020 tcgtgaagaa tcactcatga gcctcttttt gtagattcgt agtctactca tagaggcttt 70080 ttatttttct aaaaaattct taacccactt cttaacatcc aatcttaaca cacctcttaa 70140 catatcctca cagggattcg tcataagcct catacaagcc tctctaaggc gttctcttgg 70200 taagctatat gtttgctcat cttgtctgag aagtcctctg agaggggctg agaagtgcct 70260 taaaatcaat cttaacctac gtcttaacat ctcatcttaa caggcagtaa cttttcagat 70320 aattttctat tttttaaaaa ttaacagtac ctcttctcta aaccagatgc tgcaagggtt 70380 cacatcatat ctgacagggt atcttaacca ctgttcttta ctgttaattg gatagtcact 70440 ttgaagattt atgcaatttt tgacattaat tgtatatcta cttagtggcc gcttctcaga 70500 agttaaccgt tcctttgcaa attctcaaca tatctgcaac attctcttaa cagtctctta 70560 acaactctcc aaagctctta acaattcatc taatccgtta acatttaccc attctcttta 70620 acaatcttaa caggatttta acagggtctt aacaactcaa ctaatcagga ttgtcaaggg 70680 tttgaagaga atttattaga cttgacaaag gccgtttaag aggaatcacc atgttatcga 70740 aatgttaatt tgtatcgaga aattttaaca gggttgtaac agactcttaa catggccttg 70800 ttaaagaatc gttaaagagt tgtaacagga atgttaacag aatgttgtca agatgttatg 70860 cagatgactg aatagaatgt ttcagagatg ttaaaaagtt gttaacgaaa tgtaaaagtg 70920 gctaaccata gcgtaaatat tgacagtgtt ttgtccagtg tcttaaccca tcttttaaga 70980 gtctggcaag ccaacaataa gatgttttga aggctccaac aatcagcaat caatcatagt 71040 gattcatcgt agaagactct tagaagaggc ttagaagcct aacagaaagc aagtgtgaaa 71100 gcaatcattt aatgaggccg tagaatgctc ttagaagaga cttagaaggg ttaactaaccc 71160 aatcataagg ggtagactta gaaccgctta gaatgcgatt tagaagggtt atagaagact 71220 gtctaatctg gttccctgtg gggttcccac tgttagggaa ggagtggaac ggtttagaag 71280 actgtttaga agaatcacta tagcgggact atattccagt gttaacggct tagaatgctg 71340 tttagaagac aattagacga taatcaaagg aatggtaaag agtctttaaa gagaggaata 71400 cagaatatag aaagggtgga ttaagagaaa acccttaaaa cactttagcc cctgtcaagt 71460 agaatgtcaa gggctatttg ataagttact gattattgag gatttttagg ctttttatag 71520 aagtagattt tgactgctaa agttggtttt tcatcttcat cagcgatgat tagcagcttt 71580 ttagacttca tcagaagacc gtttttagca gcataagccc cgaacctgtt cacgttgtaa 71640 gagtatgtct taccatcttc aataaagtaa acgtagggct tacctaaatc gcggtgcatc 71700 aagcggcata aatcattagt tttcaagata ttcattttgt ttgttcctgt ttaaggtttt 71760 tgtttgatag ggggatttta accccctttg atgttacctg tcaagcgtcg aaagtggtta 71820 ctttgaaaga ttctggcaag ttggcttgta acagtgtgag cgtcttaaac gttgctttac 71880 tcatcctttc gataatgctt tcaagggtgt aagcagcaga cacaaaacga tgtttcatgg 71940 cggctttagc ggctaattca gtgcgatacg ttgcaaagtt accctttaaa atgtcggtca 72000 caatctcacc attttcaccc tgcatgatgt ctaaaacttc atcagcaata acaaaacgat 72060 taaggctatc tttgtgagtg tagcgcattg tttctaagga gttgtaacgg cttttaagct 72120 cctgaatctt acggcgcata gagtcatgaa tgatttctaa atacaggtca aggatggacg 72180 ctacagccgc cataaaggtt ttatgtgtgg ttgccttgtt gcctttagtg tggacaaagt 72240 aaacaccat tgaagccttg ttgattacag cgaattcacc gctaaaagag gtctggaaag 72300 tccagctttc tttgttgata cgggtcatta cagaagaaga gcggataagg ttgattgcga 72360 ttgacttttt catgttgttt attcctgttt aaggttttgt tgtggttgac tgctcaaccg 72420 atgaaaagag tatagcaaaa cggaaaaaggg gctgtaaagc cccctgatga aaataattta 72480 gtctttgtcg atttctaccc attcccctgt ttcgatgttt tcaagggtta gcgttttggc 72540 atcttctccg gcaagtacaa aacaccctgt ttcatcgtct ggaaagtcgc cacggcgggc 72600 tatacactct tctacactca cacttgacgg gacaccttgc cagctatcaa gctgtgagaa 72660 ttggcaagaa ttcaggttag aacatgatga agcaattaac aagattgttg gaaagagtac 72720 cattatttta cacctttttt ggattgatga taaattgacc gtttgcccaa atcgtgtaag 72780 attgttctaa atctttagtt gactttgtag ggcttttgtt tgtgttgtcg taaacttcga 72840 attgcaattc accattgaag cgccagaggt ttacaatctt ttcctttcca tctatcacgc 72900 cgttgtacca ttccattaca ggttgctttg tggcggtcat aatggtaaag cctaaatctt 72960 tcatcagacg ctgaacagcc ttatgttctg aatctgtcat tttcatacgt tttcaccttt 73020 ttaagaaagc tctttatcag gtgcttagac tatcaaactt ctaaacaccc tgtaaagctc 73080 tttttagttt tgaagtgctt ttaatgcgct ggcttgaagc tcttttaaca ttccagagta 73140 aatcccttgc accttatgcc ccgcttgaat gattgtttgt gcttcatcaa cttctaaagc 73200 gtaatattct gcgaaacgtt caacagtcaa gaaattatta atccagtcaa gatagatttc 73260 ttgcagtttt gccagagtga tttcttgacc actttcaagg gaaacgatac cactttgatg 73320 atttaacatg atttgattcc tgtttaaggt tttctttcga cggggttata gtctcataac 73380 cgtaacccct ttgcaatgct ttttagtgtg ttgttgcaaa gaattcaaca cgttcaataa 73440 acaggccaac cgttaaatta ttggtatatg gtaaagcggt aaaacaacgg tttacaaagt 73500 cgcttgcaac gtcttgaccg tgttttgcaa ggattaagcc agcgtgacgg gcaagggctt 73560 ctaacttgcg attatccagc ggttgagagt gtacaacaaa ctttgagcgg tcaaaattca 73620 ggaatttacg atagcaagac cattcaaaac gcatcgcttt aaatttgccg taacgtttta 73680 ctgatgcagt gcgtgaacgg tagttttgca tgtgggcaat gtgtaacatg ttttgattcc 73740 tttataaggg ttgtttgttt cgacggggtt atagtctcat aaccgtaacc ccctgtaaag 73800 ctctttttag atgttaatca gaaaatctaa atctttttct tgttcactct catctaatga 73860 atcttcaata gtttcataaa gataagcgtt tgaattctgg atagcccttg ccatttgttc 73920 aaggtgataa ctcatcgttg ccgtgtaagg gttatcaggt tgcacacaat acgggctaaa 73980 gattccgaaa aactcatact gccagatttt aagaatcatt tcaaccttaa aatcatcttc 74040 atggataaaa gaggcaatat cagccatgtt atcacaagaa taatcatcct ctaataggca 74100 atcgggaaca tcagcaagaa aaacaatcag ctttgaatca cgttcaagga ttgcggctgt 74160 tgtggtggcg tttccccttg cgtgacgttt tgcagcttca agaatagcgg cctgataatc 74220 tgcctcatcg tcaaaatcag agagttcaag acattcagct ttagcgattt tatcaagcgg 74280 gtagaaatag gttgcggcat cacattctga cacgttccat gcaccggacg gcttgccttc 74340 tccggcaatc atcattttga agttttctaa agttgtaccg tggacgcata acataatttt 74400 tattcctctt tgagggttgt tttgaaaagc tctttatctg gtgcttatgg taactaacta 74460 taaacacctt gtaaagcact tttaaacctc ttttactaaaa ataacctttt gcggctggct 74520 tgatgaatca acaatagccc aaatcagcgc gataatccag cacggaaaga aaaatagtcc 74580 gacaattgtt agaccgatta taaaaccttt gttcgtgctt tctttcttgt tggctatgat 74640 tgcaggcagt aacgccaacc acaaaacaac agcaatacac attacgacaa acaatatacc 74700 cgctaaactt tccattatca agaccttttg cttttaagtt tttcacgttc ggctaaaaca 74760 tcttcgaaag gtctaattaa tccgacacat tcaccgataa cacgtaaagc aaaaataccg 74820 atagcgccta aaacgataat cagaccaatc atttttaacc tcttaccttt tcccgatgag 74880 attctacaat ctcactgtaa tatggcttta atgtacccga accaatgaca gaaagcaagc 74940 ccgaatactg gtatttttga gcgtcattta aggccgctat aaagtcccct gcaaagttat 75000 gaccgtgagt gatagacgac atcagcaaag acgtttgcgc cgctgtaagg ccgtctaaaa 75060 acgatttaac agcccctttg tttgcgtcta cctgcttaac ctctttgatg aggttgaaca 75120 agtaaaaacc gcccactgta agagcggcaa ggccgccccc cataagatag tttaccgttg 75180 attgtgtcac gttatgccgc cactttgcga gatttacgga aagtcacttt aacaggttta 75240 actttgtctg tcacgctgtt aacctgctgt acaacttcaa cacgtttggc gcggataacc 75300 tttgcaaagt cacgtgacca tcgtttagtt gcttctttgc cgttgtcagt tgctttaccg 75360 aaagtagcag aacgcatagc agaacgagaa gagaagaaaa ttttcatgat gtttattcct 75420 atttaagagt tagtaagatt agtaaccaga ttgtgccaga aaatcagatt tcaagtcaag 75480 cataaatttt ctgttttcgg catctttcca tagtgcatca ctgataccgt gtgaaacgat 75540 gttagtaaac ccgtaatcac atactaacac ggttttaacg gcgaattcaa acgctttaga 75600 ttcattcatc gcatagttag taccgcgttt catgatgtcg tcaatttcag cgataacacc 75660 ttgcgtaaat tctacgtttt ccaccgattt tgtcaagttc aaaacgttac ttgaaaaacg 75720 ctcacgatta gcgggaaacg ttgcaaggga ttgacgggca acctttacaa gttcttgggc 75780 gctgaatctt tcagcagctt tagaatcatt tgaggcaacc agtgaacggc taaaagtttt 75840 cggtgaaaga atcatgttag tttcctttat aagggttgat tgataaagtt ctttatcagg 75900 tgcttagact atcaaacttc taaacaccct gtaaagctct ttattacatt acagcaatct 75960 ttttaccgtt tacgtttatg tgtaggcttg cagcgttacc gtaacaagtt gaataaatac 76020 ggcgcaaacg tccagcccaa ttgacagccc acggcgtagg gatatttttt ccgtaaccac 76080 ttttagtttt agaaagtccg gcatcatgcc accacaaagg cttatattca gcacgtaaat 76140 cagtaaactg catttccttt ccgtgcccct ctcgcaaagt aatatccata acagggggaa 76200 gattattgta agtagtaaca ttcattattt tttcctttat aagggttgtt tgtttcgacg 76260 gggttatagt ctcataaccg taaccccttt gcaactagat ttttgagatt cttttcttga 76320 actcttcaac ggcttcaatc atttgcgttt cacaatctga atcttttacg ttacgctcta 76380 aagattcttc taatgattct tcatcaagat aagagtaatc aaaaccgtaa cccattgcaa 76440 cagaattaat cacctcgttt ccttcaaggt caaagattgc acctgtaatg aaatattcag 76500 ccgcttcaat gtcacgcttt aattgtgcct gtaagctgtc ataagccgct ttagatgggt 76560 tatcccttcc ctgttctgca tactccgctg taagctgtgc aaggctgcat tgcaacggga 76620 ttgcataagg ataactattg cgacaagcta aagggttttt gatagtcacg ccgccctgat 76680 gattgtttgt tagtttccac tggtcactaa agaaagcagg gcaacattca gacatttcag 76740 cagtgaaacg gatttcaaaa gttggcttga tgattgagaa cgtagcattc attttcttga 76800 ttcctttata agggttgttt gtaaaagctc tttatctggt gcttatacta tctaagtaca 76860 aacaccatgt aaagcacttt ttagtaattt aaacgtgtta cttttgaaac tttgtcaccc 76920 ttgtgacggc taacagttac cgcaaattta cggcttgaat ctcgtttccg tgacacaagg 76980 tttttaatct tcaattcaat caagctgaat aacggcgctt tgatttcgta accttcaacc 77040 gtggcgctat agtacaagtt accgtttgca gcgtcaacaa ccttttgaag attttctaaa 77100 gattttaatc tgtaagtgta acggattgcg ccggatgtct cttcataacg ttgaacaagt 77160 tcaaccggat aacccaactt ttcaaggctg tatgtaatgt cgccattatc tttaccatac 77220 ccgacttgtt gacggcgtga accacttccc acgatgtcaa acggatgata atatttgcca 77280 gcctttttat catcaatcat tacagtaacg caataatgcg ccgtgcggtt gccgtacacg 77340 tcccgcgatt gtgaatactc acgggcttct aaaaagattt cagattgaag gtttaagccc 77400 tcgcaataga tacccatgcg atttaaaaac ttttgaatcg tgacaccttc ccgaatgttt 77460 ttaccttctt ttgagattga agggttgaaa gagttatcag caagaatcgc acgttgttta 77520 gcgttaacaa taaatggggt attcataatc ttttatcct tgtttagggt tgctttgctt 77580 cgatggggtt actttaccaa aaccataaaa ccattgtcaa actgtttttc taaaaacttt 77640 tctctttctt catcaacagg gaaaagctct ttatagtagt tgtctgcatc ttcataacag 77700 ccgaaaacaa ttacattctt atcagcacgt gtaatgataa cgtgtgacac ggtagcaagg 77760 attttttcat cagggcaacg aacaacacac tgtttaaaat aactgtatgc gtcctcgtta 77820 gacactttag aattaaccgc tgtatgataa gagtgtttag ccggattgca tacaacatta 77880 atagggcatg ttgagttaac tacttcttta cacattcttg taaaagggtc tcgcattttc 77940 tttatccgt tgtggttgtt tcgatggata gaaatataaa gattgtcctt tctaaagtca 78000 acaaaaaata aaagaaaaag tttttaaaaa atcgcttgac gttattccgg tgaatcgctt 78060 atactatttc acatgttgag gggctggcta agtctctgaa tgtaaagaca aaatgcttac 78120 agggctgaaa atgatactac aaccggacga tatttcggta catggtagag gggagggtgg 78180 gcgttattta caggaatctt tctctatgca caaattcaaa atgaaaaacc tctccagacc 78240 ctctatgcac aaatcgaaaa tgatttttct tgccagacct tatgcacgat tttgaaatga 78300 aaaacgttgc cagtccctat gcacaaattg aaaatgaaaa agctatccgg taatagaaat 78360 aaaaaagccc ccgattaagg aggctcttta atatattaat caggtatctc atcacccaat 78420 tgaatattta attgttgtgc tccccaattc tcagcgggaa taataagaac actttcaagt 78480 acacgttcaa acttattagt gtagactttg ccacctaatt taccttcacc taaacatttg 78540 agcttactca gcttctgatg ctggaggggt aacttggagg gtaactcatc aacacgccct 78600 tctttacaca ccgagaagaa tagtccatct gattcaagga aggtgtaacg tggattggta 78660 ggtgtctttg gcttgaactg tttgctgatg atgaaattat taacatcgct tggatggcgc 78720 acgaagtaaa tatcaccacg aacaagaaga gaatcagcca gactaatgac cgcattaata 78780 tccagacccc cgataccagc cccaataaac ggtaagccta agcgataacc ttcaccaaca 78840 tgctcaacca taaagtcatc aaggtgtttg aaggcttcag ccatagcgaa gtagtcagcg 78900 ttgcgaccac cgttaaattg ggtatagagg ttcactacaa ggccgtcatt tgtatgagca 78960 acggacagct tacccaacat ctctttatga gcgtccttct cattgtagta atcataagcc 79020 tgtgtatcag cagcaaagca ttgcggataa aactttgcaa agtgtactgc aataccagca 79080 cccatcatat tgtgacagtt acatccttga ccaattgcat gaaaattacc tttacggaat 79140 tctgcaaaca aatcaccagt gaaaatgtca acgaccatgt gaacctcagt tttgttgttt 79200 caggatgaca tcaatcaacc cgttataagg aaccgcgcaa gtaaaatact tgtcacgcca 79260 atattcaaga cttgatgtta ctgcaagacc gtctgtcccg tcaagctgtt tcaactttgt 79320 cccgtcagtt tcctgttttt gtagtaggtt tgcttgaacc actggctttt gctgcaaagt 79380 tggctgttgc ttcgttgaac atgatgcggt agtcatcact aacacacttg ttaagaaaaa 79440 caggttgaat aatcttttca tgtgttaccc tctcattgaa tgattgattg ttttgcttcc 79500 attggtcaag tgaattgaca acattggttg cgatttcttt ttggtcattc tggaattgac 79560 cacctgcttt agtagccgct ttctctgcaa ccagttccag tgctgattga tgccagtttg 79620 atgctttata tccagcaagg aaagacacta ccagtagtgc aataagtaca tagtttttca 79680 tttaaccctc tacgagaaaa ggactccgaa gagtccattt tatttagaag tttggcaggt 79740 ctgcttttcg ttcacgtgcc agcttttgtg cggtgaacag tgacagaagc aggtcattag 79800 gtttccacgg ttcgaatacc acgaacttat taacctgctc aacaacttca ccaatctctt 79860 tgtgattcat ccaccactca cggcgggttg tatcagcgat aatcactggc attttatgct 79920 ctaccagagt acccttctca tcacgaccgt agcgagtctc ttcagtctgg atagggtcat 79980 cgaagtcacg atgatagaac tgattgatgg ttacgccatt atccatgtga agctgaacaa 80040 ggttcagttc attcaccaga ctatgaagtt taccaaaaga ctctttcaga tgctgaagca 80100 agccatcatc catcctctca gacacaatct tctcaggatg ctgagatgcc atgtgtaaga 80160 tagccttacc aacatcctca gaggtgtaag ggaaagctgt gaatcggtga tgggtagaga 80220 atgaaacgtg agcttcatcc atgacgactt cctgactctt accatcaatc atgcaaaacaa 80280 tcttattcat gttaaaccct tttcatcaaa aatttattca gttgtgaact cactggagca 80340 aggaactcca atatgttgat atgatgccct acaccacgtt tacctgtcaa gtaataaatg 80400 gcatgttcaa gtcttcccaa atccttacag gagattgcgt tgttcacaaa gatgatatgc 80460 tcacctctgt aaacagcagt cttaataatc cctttgtaga agtccttgac atcaatcctt 80520 gggaactctt ctccgaagtg atgccttaca ctgtggtcat tgcagttaag gtagccaaac 80580 ttatcacgtt caagatactt gtagacctca gattgcaagg cattcggtgc atctttatag 80640 actaccagac gaagcattac gcagtacctt tcttgtcctt gtaagcacgg aaccagaact 80700 ctgtaacagg cttaccattc atgtgaatca acccatgagc taagtcaaag cgacgatggg 80760 aaatccatgc taccagacta tcatcaccga tgtcaaacag tgcttgcagc tttacctgag 80820 aaggtttgat acggtaacgt gtagcaaact ctttgatgat gaagtctgcc aactcattat 80880 tgtcagcctc ttcagatacc ataatccctg ttgtggtttc aaggattgga aagatgattt 80940 cacacttcat atttattcct tactaaaaag gactccgaag agtcccttga ttgattagct 81000 gctcatcgct aaatcgtaac cttcccaatt atcaacaccg tgtgcttcca gtgcatccaa 81060 cttttcaagt ctttcgtcct gctcttcaag atggtcacac aacttttgac gtaatgccaa 81120 agccatgtca ttctcttcag agttgagatg agacagaagg tcgtcaaagt cctgaatcag 81180 gctactcatt tcagagcgcc aagcaggatt gcaacagact gattggtaat gcttgagctt 81240 gcgtcaacag cagctttgat tttcgcatca gcaatttcag atttagttgc ttcgtcgatg 81300 aagacgtaag tagttttctt gtcgcccttt acaaactcca gcatcagagt tgtttccatg 81360 ttgaatgaac ggtaaggagc gccagcggcc tgaataccct ctgcaccgag agttgaagcc 81420 agacccatgt catagatggt cagcagttca ggtttgtgtg cggttgttga accagcacct 81480 tttacatgct tggaaacacc agtcagagca cggtattcac ggagcgcacc atctttcttt 81540 acgttgacag cgcgggaaaat acggcctgag aactgagctt ttacaacatc agcaacgtat 81600 gccactttgc ttgacaggtc attgatgttt actacgatag tttcctgaat catgataaag 81660 cctcttagtt agttgtttgt cgtcttgaca ggagagatta tgccaccctt cttagagtgg 81720 cgtcaactac tttttacgaa tgatacagca ttgttttgat ttcagggaaa tcctgattaa 81780 tctgctgaat attcaacgca gtatcatcaa taaacaattc aacatcgtag tatggcataa 81840 tgcaggtgtg caaaatgtca cgcttcagga tgtgtgcagg tgtcaggtta tcaaataagc 81900 cacgcatgaa cagatgatac ttgtagggat tgaacacatt gttcagtgtc atcgttgttg 81960 agatacgttg tgactctcca cgtgcagtca ggaagattaa ggtatcacct ttctcaacac 82020 gacccttaac gaggtctaca ataatccctt gctcatgagc catctctgca atggtcatgt 82080 gattctgcca atatggttcc cgttcaccag taataagcag agtgccttca tcgtcaaaat 82140 gggtgacaac accatcaata tcaacaacga ttgctttagg cttcttgtca taaccttctg 82200 gcatcttttc aggaaggata gccaactggt tcatgaacac ttcttcctct gacaactctg 82260 gagcttcacc ctctttccag atacgaacct ttccagataa gttgctgtgg tgaatatcac 82320 agattgccgc ttgatatact gaatcaagac gtgaacgtac tgcgacttta caagtctgct 82380 gaatcatgat taatcctctt tgttaatgat acagtgctct acaatcttca tcttttgctt 82440 tacctgtttg gcatagcctt tgccagccga tacattgtaa cctgcattgt acgaagccag 82500 aacacgtgtt aggttgtttg attgtcctgt tttaaggtaa tatctgccgt tccaatagtc 82560 aagttcattc ttggcttgtt cagcggaata atcgaaacta tgaataagtt tctttttggc 82620 aactgctggt ttttcaccag tcctattagt taccgttgtg agcttattct ggaaaacacc 82680 ataagaacct ttgtgattga caaccttatc gccagcactg gattcttgat aggaaatggc 82740 cgctaagacc actccccaat taccacccat gttatgtgaa ccataggcgt gagccttttt 82800 taaaactgca acctgaccct gagagaactc tggacagtct actgctttaa ctccaaacgg 82860 tgtaatacag agcgccccaa caaggataag ttgtttcagc attctctttc ttgcctgttt 82920 atagttaaac gaagacacat gctaacacac ttttcgtggt tggtctaccc tcttgtacgt 82980 tttgtggcaa ccattgcatg agtaaacaga caagtcattc ctgtcaggtt tgtggaaaca 83040 gttgcagcca cagtcacaaa agaagtcttt accaccgact aagagagtaa atgatttcac 83100 acaaccttca tcatcaagga ctgctttatc tttgtctatt cgcatcgtac ttctccttca 83160 tcttttggta gttgtcgtct gttttaagcc actcgatagc tttaacaata acatcaccgt 83220 ggcatttgtg aggtgaacag taacagacca gtttctttcc gtcaagagaa agaatcagtg 83280 cagggtcaat agtcccattg ttcataccac gccagagata actctcatac ttccgtatca 83340 cagtatcacg gtcacactta tctgttatct tgaaagggtt gtgtagcggg tgttctttca 83400 agtcctgtcc agcccttcca atatacacat aaccctctgg aatcctctta ccaatgtggt 83460 agaagttgtg tacagttgtt tcctgaatgg tttcttccag tttcaaaacc tgaatcgggc 83520 cagcccaaag ataaaacatgg tcacggtaac gtgcgtctga acgaggccac caccaatcac 83580 cttcctgaaa gaatagaatc tggttgtcag caaccggaag cgttataggt tcggggttaa 83640 ctttgcaaag gtaaagtccc atcgcttttg gcttaccctc tctaaactca atcatacttc 83700 cctccaaata aaaaaggact ccgaagagtc cctatatctt aatgccaacg gctagtgcgt 83760 gacaagagtt tctttgatga tttctcaaat tgcaatcgtc cgaagaggat gataaagaca 83820 actgcaatga tgaagattac cacaccacca aggccatttg aaatcagtga tgaccagaat 83880 ggtacatcaa gtttgaagat ttcgtacagg aacttgacaa tggcaacagg ccaagtcagt 83940 atcgcaacga gataagacag tacaacaatc agatagtaca tttcaattcc tcttagaaga 84000 ggggctttcg ccccaattca tttagacacg tggtttacgg gaaatcagtt caccagcaga 84060 gccgacaaca accacttctg aagccagacc tttcaggttg gtcacgtcga tgattttacg 84120 gttgctgtca accacaccga caacttcata ctggcaagaa cgcatcttct ggtcattgta 84180 gtctgttggt acagatacaa cgtcacgtgg atggacacga accttgatag tggtgtcgcc 84240 agagaatgaa cgtacataat cccaagcacc aacatgcaga ccctgtgaac aggtaacgtt 84300 tttgtctgcg tcaaccatcc agcgaggcat tgacacgatg ttaccaatgt tgttaggaac 84360 tttactggtg cggctatcaa ccagaccgtt accgttgtta cgaac 84405

Claims (20)

A bacteriophage with accession number KACC 97039P, which has specific lytic activity on one or more bacteria selected from the group consisting of Erwinia amylovora and Erwinia pyrifoliae.
The bacteriophage according to claim 1, wherein the bacteriophage has a lytic activity specific to Erwinia amylovora.
The bacteriophage according to claim 1, wherein the bacteriophage has a base sequence of SEQ ID NO: 1.
The bacteriophage according to claim 1, wherein the bacteriophage is of the Myoviridae family.
The bacteriophage according to claim 1, wherein the bacteriophage is active at 30°C to 70°C.
The bacteriophage according to claim 5, wherein the bacteriophage is active at 40°C to 60°C.
The bacteriophage according to claim 1, wherein the bacteriophage is active at pH 3 to 12.
The bacteriophage according to claim 7, wherein the bacteriophage is active at pH 4 to 11.
A pesticide composition comprising the bacteriophage of claim 1 as an active ingredient.
The pesticide composition of claim 9, wherein the bacteriophage is used for the prevention or treatment of one or more diseases selected from the group consisting of fire blight and black blight of fruit trees.
The pesticide composition according to claim 9, wherein the pesticide composition further includes an excipient.
The pesticide composition according to claim 11, wherein the excipient is a surfactant or an extender.
The pesticide composition according to claim 9, wherein the pesticide composition is in one or more forms selected from the group consisting of wettable agents, granular wettable agents, liquid wettable agents, liquid formulations, water-soluble formulations, water-soluble granules, and encapsulation agents.
An antibiotic composition comprising the bacteriophage of claim 1 as an active ingredient.
A disinfectant containing the bacteriophage of paragraph 1 as an active ingredient.
A detergent containing the bacteriophage of claim 1 as an active ingredient.
A method of controlling one or more diseases selected from the group consisting of fire blight and black blight of fruit trees using the bacteriophage of claim 1.
The method of claim 17, wherein the fruit burn disease is caused by Erwinia amylovora, and the fruit tree branch black blight is caused by Erwinia pyrifoliae. How to control .
Use of the bacteriophage according to paragraph 1 for the prevention or treatment of one or more diseases selected from the group consisting of fire blight and fruit tree blight.
Use of the bacteriophage according to claim 1 for the manufacture of a pesticide composition for the prevention or treatment of one or more diseases selected from the group consisting of fire blight and black blight of fruit trees.