KR20230085021A - Composition for Hair Growth Stimulation or Hair Loss Prevention Using an Extract of Artemisia gmelinii - Google Patents

Abstract

The present invention discloses a composition for promoting hair growth or inhibiting hair loss using an extract from a hot dog having an activity that promotes the proliferation of human dermal papilla cells and human hair cells.

Description

Composition for promoting hair growth or inhibiting hair loss using an extract from a hot summer season {Composition for Hair Growth Stimulation or Hair Loss Prevention Using an Extract of Artemisia gmelinii}

The present invention relates to a composition for promoting hair growth or inhibiting hair loss using an extract of Artemisia gmelinii .

Hair is formed in hair follicles. There are about 5 million hair follicles in adults, which are distributed throughout the body except for the palms and sea gourds of human skin. (Bulletin of Food Technology 2009, 22(4):651-663). Scalp hair accounts for about 20% of human hair, and it has physiological functions of hair such as evaporation of sweat and temperature control, as well as the function of protecting the head from shocks through the buffering action of the head. As a result, it has an important social function in individual personality and image production (Clin Dermatol 2001, 19(2):161-166).

Each hair grows from the origin of the hair follicle, and the hair follicle is composed of dermal papilla cells, hair germinal matrix cells, outer and inner walls of the hair follicle cells, and bulges. do. Hair repeats the growth cycle of anagen, catagen, and telogen. During the anagen phase, as hair dermal papilla cells and human hair germinal matrix cells develop, The keratinocyte is keratinized and hair grows, the catagen phase is when cell division stops and hair growth stops and the shape of the hair is maintained, and the telogen phase is when hair cells start to become active and new hair is generated. It's time to shed old hair. Therefore, factors affecting the proliferation of dermal papilla cells or hair hair cells in hair generation, growth, and dropout are important targets for improving hair loss or hair growth.

On the other hand, alopecia refers to vellus hair of terminal hair or loss of hair that should normally exist, and usually about 50 to 100 hairs fall out per day, but if more than 100 hairs are likely to be alopecia. Alopecia is divided into scarring alopecia, which is permanent hair loss in which hair follicles are damaged and hair does not regenerate, and non-scarring alopecia, which can be treated by maintaining hair follicles (Bonghyun Ju et al., 2014). As human life expectancy increases, alopecia is considered a disease rather than an irresistible force, and many people are interested in preventing hair loss and growing hair (Ann N Y Acad Sci. 1991. 26(642): 11).

The exact mechanism of hair growth and hair loss has not been fully elucidated to date, but the causes of hair loss include deterioration of hair follicle function due to the involvement of male hormones, deterioration of the metabolic function of the hair follicle and hair bulb, local blood flow disturbance due to scalp tension, malnutrition, Stress, drug side effects, genetic factors, autoimmunity, local infections, chemicals, diseases such as leukemia, tuberculosis, and abuse of hair products are very diverse.

Currently, the most commonly used drugs for hair loss treatment are minoxidil preparations (see US Patent Nos. 4,139,619 and 4,596,812) and finasteride (US Patent Nos. 5547957, 5760046 or Korean Patent No. 375083).

Hair loss treatment requires long-term treatment, so minimizing side effects is key. Accordingly, efforts are being made to discover new hair growth ingredients or materials from natural products that have relatively few side effects on the human body.

Thus, the present invention discloses the hair loss inhibitory activity of the deowijigi extract.

An object of the present invention is to provide a composition for promoting hair growth or inhibiting hair loss using an extract from a heat exchanger.

Other objects or specific objects of the present invention will be presented below.

The present invention was completed by confirming that, as confirmed in the Examples and Experimental Examples below, the extract of the heat group promotes the proliferation of human dermal papilla cells and human hair cells.

The present invention is provided based on these experimental results, and the composition for promoting hair growth or inhibiting hair loss according to the present invention is characterized in that it contains an extract from a hot dog as an active ingredient.

In the present specification, "extract" means the stem, leaf, fruit, flower, root, etc. of a plant to be extracted, water, lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, butanol, etc.), methylene chloride, ethylene, acetone, hexane, Extract obtained by leaching using ether, chloroform, ethyl acetate, butyl acetate, N,N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol or a mixed solvent thereof , refers to an extract obtained by using a supercritical extraction solvent such as carbon dioxide or pentane, or a fraction obtained by fractionating the extract, and the extraction method includes cooling, reflux, heating, and ultrasonic radiation in consideration of the polarity of the active substance, the degree of extraction, and the degree of preservation. , supercritical extraction, etc. may be applied. In the case of the fractionated extract, after suspending the extract in a specific solvent, the fraction obtained by mixing and standing with a solvent of different polarity, and the crude extract are adsorbed on a column filled with silica gel, etc., and then a hydrophobic solvent, a hydrophilic solvent, or a mixed solvent thereof It is meant to include fractions obtained as a mobile phase. In addition, the meaning of the extract includes a concentrated liquid extract or solid extract from which the extraction solvent is removed by lyophilization, vacuum drying, hot air drying, spray drying, or the like. Preferably, it refers to an extract obtained by using water, ethanol or a mixed solvent thereof as an extraction solvent, and more preferably an extract obtained by using a mixed solvent of water and ethanol as an extraction solvent.

In addition, in the present specification, the term "hair loss" should be understood as including all symptoms classified as hair loss in the art regardless of direct or indirect causes of its occurrence. Specifically, it means to include all symptoms of hair loss due to poor blood circulation, excessive secretion of sebum due to excessive production of dihydrotestosterone, deterioration of scalp function due to peroxide or bacteria, aging, genetic factors, stress, and their combined effects. .

In addition, in the present specification, "active ingredient" means a component that exhibits the desired activity alone or can exhibit activity in combination with a carrier having no activity itself.

The composition of the present invention may be included in any amount (effective amount) according to the purpose of use, formulation, blending purpose, etc., as long as the active ingredient can exhibit ankle promoting or hair loss inhibiting activity. A typical effective amount is based on the total weight of the composition. It will be determined within the range of 0.001% by weight to 20.0% by weight when Here, "effective amount" means that when the composition of the present invention is administered to a mammal, preferably a human to which it is applied, during the administration period according to the advice of medical experts, etc., it can exhibit the intended functional and pharmacological effects such as hair growth promotion. It refers to the amount of the active ingredient included in the composition of the invention. Such an effective amount can be determined empirically within the ordinary skill of the skilled artisan.

In addition to the active ingredients, the composition of the present invention has already been proven safe in the art in order to increase or reinforce the effect of promoting hair growth or inhibiting hair loss, or to enhance the convenience of intake or intake through the addition of similar activities such as blood pressure control activity. and may further include any compound or natural extract known to have that activity. These compounds or extracts include compounds, extracts, and pharmaceuticals listed in compendia such as each country's pharmacopoeia (in Korea, the "Korean Pharmacopoeia") and each country's Health Functional Food Codex (in Korea, it is the "Health Functional Food Standards and Specifications", a notification of the Ministry of Food and Drug Safety). In accordance with the laws of each country regulating the manufacture and sale of products (in Korea, it is the 「Pharmaceutical Affairs Act」), the laws of each country regulating the manufacture and sale of compounds or extracts and health functional foods (in Korea, the 「Act on Health Functional Foods」) ”), compounds or extracts whose functionality is individually recognized are included.

For example, dexpanthenol, biotin, L-menthol, zinc pyrithione, etc., which are ingredients that have the function of alleviating hair loss symptoms listed in the "Functional Cosmetics Standards and Test Methods" of the Ministry of Food and Drug Safety according to the Korean Cosmetics Act, L-glutamic acid-derived GABA-containing powder, katsuobushi oligopeptide, nattobacter cultured powder, Seomoktae (swine-eyed soybean) peptide complex, salmon peptide, Olive leaf extract, sardine peptide, casein hydrolysate, coenzyme Q10, grape seed enzymatically digested extract powder, Haitai oligopeptide, etc. may correspond to these compounds or extracts.

In a specific aspect, the composition of the present invention can be understood as a food composition.

The food composition of the present invention can be prepared in any form, for example, beverages such as tea, juice, carbonated beverages, and ionic beverages, processed oils such as milk and yogurt, chewing gum, rice cakes, traditional Korean snacks, bread, snacks, noodles, etc. It can be manufactured into health functional food preparations such as foods, tablets, capsules, pills, granules, liquid, powder, flakes, pastes, syrups, gels, jellies, and bars.

In addition, the food composition of the present invention may take any product classification as long as it conforms to the enforcement regulations at the time of manufacture and distribution in legal and functional classification. For example, health functional food according to the Korean 「Health Functional Food Act」, or snacks, legumes, teas, beverages according to each food type according to the Food Code (MFDS notification 「Food Standards and Specifications」) of the Korea 「Food Sanitation Act」 , special purpose foods, etc.

The food composition of the present invention may include food additives in addition to the active ingredient. Food additives may generally be understood as substances that are added to, mixed with, or infiltrated into food in manufacturing, processing, or preserving food. Since food is consumed daily and for a long period of time, its safety must be guaranteed. According to the Food Additives Code under the laws of each country governing the manufacture and distribution of food (in Korea, it is the 「Food Sanitation Act」), safety-guaranteed food additives are limitedly regulated in terms of ingredients or functions. In the Korean Food Additives Codex (MFDS notification 「Standards and Specifications for Food Additives」), food additives are classified into chemical synthetic products, natural additives and mixed preparations in terms of ingredients and are regulated. It is divided into additives, preservatives, emulsifiers, acidulants, and thickeners.

Sweeteners are used to impart appropriate sweetness to foods, and both natural and synthetic sweeteners can be used in the food composition of the present invention. Preferably, a natural sweetener is used, and examples of the natural sweetener include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose, and maltose.

Flavors are used to improve taste or aroma, and both natural and synthetic flavors may be used. Preferably, it is the case of using a natural one. In case of using natural ones, in addition to flavor, the purpose of enhancing nutrition can also be combined. As a natural flavoring agent, it may be obtained from apples, lemons, tangerines, grapes, strawberries, peaches, etc., or obtained from green tea leaves, roundworms, bamboo leaves, cinnamon, chrysanthemum leaves, jasmine, and the like. In addition, those obtained from ginseng (red ginseng), bamboo shoots, aloe vera, ginkgo, etc. can be used. Natural flavors can be liquid concentrates or solid extracts. In some cases, synthetic flavors may be used, and as synthetic flavors, esters, alcohols, aldehydes, terpenes, and the like may be used.

As preservatives, calcium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate, EDTA (ethylenediaminetetraacetic acid), etc. may be used, and as emulsifiers, acacia gum, carboxymethylcellulose, xanthan gum, pectin etc. may be used, and as an acidulant, acidulic acid, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, phosphoric acid, and the like may be used. Acidulants may be added to the food composition to have an appropriate acidity for the purpose of inhibiting the growth of microorganisms in addition to improving taste. As the thickening agent, a suspending agent, a sedimentation agent, a gel forming agent, a swelling agent, and the like may be used.

In addition to the food additives described above, the food composition of the present invention may include physiologically active substances or minerals that are known in the art and whose stability is guaranteed as food additives for the purpose of supplementing and reinforcing functionality and nutrition.

Examples of such physiologically active substances include catechins contained in green tea, vitamins such as vitamin B1, vitamin C, vitamin E, and vitamin B12, tocopherol, dibenzoylthiamine, and the like. Examples of minerals include calcium preparations such as calcium citrate and magnesium stearate. magnesium preparations such as the like, iron preparations such as iron citrate, chromium chloride, potassium iodine, selenium, germanium, vanadium, zinc, and the like.

The food composition of the present invention may include the above-mentioned food additives in an appropriate amount to achieve the purpose of addition according to the type of product.

Regarding other food additives that may be included in the food composition of the present invention, reference may be made to national food codes or food additive codes.

The composition of the present invention can be understood as a pharmaceutical composition in another specific aspect.

The pharmaceutical composition of the present invention may be formulated into an oral formulation or a parenteral formulation according to the route of administration by a conventional method known in the art, including a pharmaceutically acceptable carrier in addition to the active ingredient. The route of administration herein may be any appropriate route including topical route, oral route, intravenous route, intramuscular route, and direct absorption through mucosal tissue, and two or more routes may be used in combination.

Pharmaceutically acceptable carriers are well known in the art depending on the route of administration or dosage form, and specifically, reference may be made to the pharmacopoeia of each country including the "Korean Pharmacopoeia".

When the pharmaceutical composition of the present invention is prepared as an oral dosage form, powder, granule, tablet, pill, dragee, capsule, liquid, gel, syrup, suspension, wafer according to a method known in the art together with a suitable carrier. It can be prepared in formulations such as Examples of suitable carriers include sugars such as lactose, glucose, sucrose, dextrose, sorbitol, mannitol, and xylitol, starches such as corn starch, potato starch, and wheat starch, cellulose, methylcellulose, ethylcellulose, sodium carboxymethylcellulose, Celluloses such as hydroxypropylmethylcellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, magnesium stearate, mineral oil, malt, gelatin, talc, polyol, vegetable oil, ethanol, Serol etc. are mentioned. In the case of formulation, appropriate binders, lubricants, disintegrants, coloring agents, diluents, etc. may be included as needed. Suitable binders include starch, magnesium aluminum silicate, starch ferrite, gelatin, methylcellulose, sodium carboxymethylcellulose, polyvinylpyrrolidone, glucose, corn sweetener, sodium alginate, polyethylene glycol, wax, and the like, and oleic acid as a lubricant Sodium, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride, silica, talcum, stearic acid, magnesium salts and calcium salts thereof, polyethylene glycol, etc. may be mentioned. As disintegrants, starch, methyl cellulose , agar, bentonite, xanthan gum, starch, alginic acid or its sodium salt, and the like. Examples of the diluent include lactose, dextrose, sucrose, mannitol, sorbitol, cellulose, and glycine.

When the pharmaceutical composition of the present invention is prepared as a parenteral formulation, it may be formulated in the form of an injection, transdermal administration, nasal inhalation, and suppository along with a suitable carrier according to a method known in the art. When formulated as an injection, an aqueous isotonic solution or suspension may be used as a suitable carrier, and specifically, an isotonic solution such as phosphate buffered saline (PBS) containing triethanolamine, sterile water for injection, or 5% dextrose may be used. . When formulated as a transdermal formulation, it may be formulated in the form of ointments, creams, lotions, gels, external solutions, pastas, liniments, air rolls, and the like. In the case of nasal inhalation, it can be formulated in the form of an aerosol spray using a suitable propellant such as dichlorofluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide, etc., and when formulated as a suppository, the carrier is Witepsol ( witepsol), tween 61, polyethylene glycols, cacao fat, laurin fat, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene stearates, sorbitan fatty acid esters, and the like can be used.

Regarding the specific formulation of the pharmaceutical composition, it is known in the art, and for example, reference may be made to Remington's Pharmaceutical Sciences (19th ed., 1995) and the like. These documents are considered as part of this specification.

The preferred dosage of the pharmaceutical composition of the present invention is in the range of 0.001 mg/kg to 10 g/kg per day, preferably 0.001 mg/kg to 1 g, depending on the patient's condition, weight, sex, age, severity of the patient, and route of administration. /kg range. Administration can be done once a day or divided into several times. These dosages should not be construed as limiting the scope of the present invention in any respect.

In another specific aspect, the composition of the present invention can be identified as a cosmetic composition.

Even when the composition of the present invention is identified as a cosmetic composition, the cosmetic composition may be classified as an arbitrary product in terms of its use and law, and specifically, for purposes such as alleviating hair loss, promoting hair growth, improving skin troubles, and improving atopic dermatitis. It may be a functional cosmetic, a general non-functional cosmetic, and the like. In product form, it may take any product form, specifically, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, shampoo, surfactant-containing cleansing, oil, powder foundation, emulsion foundation It may take the form of a product such as a wax foundation, a spray, or the like. In a specific product form, it may be a softening lotion, nutrient lotion, nutrient cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder formulation.

The cosmetic composition of the present invention may include, in addition to the active ingredient, ingredients commonly used in cosmetic compositions, for example, stabilizers, solubilizers, surfactants, vitamins, conventional adjuvants such as pigments and fragrances, and carriers.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane / May contain a propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, specifically water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol, fatty acid esters of sorbitan, and the like may be used.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, or polyoxyethylene sorbitan ester, microcrystals Star cellulose, aluminum metahydroxide, bentonite, agar, and the like can be used.

When the formulation of the present invention is surfactant-containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The cosmetic composition of the present invention may be prepared according to a method for preparing a cosmetic composition conventionally performed in the art, except for including an active ingredient exhibiting a hair growth promoting activity.

As described above, according to the present invention, it is possible to provide a composition for promoting hair growth or inhibiting hair loss using an extract from a deodorant.

The composition of the present invention can be commercialized as food, medicine, hair cosmetics, and the like.

Figure 1 is a result showing the effect of promoting the proliferation of human dermal papilla cells of the heat gijigi extract.
Figure 2 is a result showing the effect of promoting the proliferation of human hair cells of the heat gijigi extract.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these Examples and Experimental Examples.

<Example> Preparation of hot air extract

Heat machine ( Artemisia gmelinii Weber ex Stechm.) 500 g of dried powder of the aerial part was extracted with 5 L of 50% ethanol for 48 hours twice. After filtering the extract with a filter (Advantech No. 1), the filtrate was concentrated under reduced pressure at 40° C. to obtain an ethanol extract. The extract concentrated under reduced pressure was lyophilized to obtain an extract in powder form.

<Experimental Example> Evaluation of the proliferation effect of human dermal papilla cells and human hair cells

<Experimental Example 1> Evaluation of human dermal papilla cell proliferation effect

The effect of the extract on cell growth of human hair dermal papilla cells was evaluated as follows.

Human dermal papilla cells (Innoprot, cat. no. P10881) were placed in MSC (Mesenchymal Stem Cell) medium (Innoprot, cat. no. P60115), a dedicated medium containing 5% FBS (fetal bovine serum) and MSCGS. × 10 ⁴ cells / was inoculated into a 96-well plate and cultured for 24 hours at 37 ° C. under 5% CO ₂ wet conditions. The cells were treated at concentrations of 25, 50, and 100 μg/ml and cultured for 72 hours As a positive control, 10 μ of minoxidil (sigma) was treated and cultured in the same manner as above.

Then, 20 μl of MTS (CellTiter 96ⓡ AQueous One Solution Cell Proliferation assay kit, Promega) reagent was added, and after incubation for 2 hours in a cell incubator, the degree of cell growth was measured by measuring absorbance at 490 nm using an ELISA reader.

The proliferation effect of the human dermal papilla cell proliferation of the heat group extract was shown in FIG. 1, and the heat group extract showed a concentration-dependent effect of human dermal papilla cell proliferation.

<Experimental Example 2> Evaluation of human hair cell proliferation effect

The effect of the extract on cell growth of human hair germinal matrix cells was evaluated as follows.

MSC (Mesenchymal Stem Cell) medium ((Innoprot, cat. no. P60115), a dedicated medium containing 5% FBS (fetal bovine serum) and MSCGS, hair cells (Innoprot, cat. no. P10882) 1 × 10 ⁴ cells/ was inoculated into a 96-well plate and cultured for 24 hours at 37° C. under 5% CO ₂ wet conditions. The cells were cultured for 72 hours at concentrations of 50 and 100 μg/ml, and as a positive control, 10 μ of minoxidil (sigma) was treated and cultured in the same manner as above.

Then, 20 μl of MTS (CellTiter 96ⓡ AQueous One Solution Cell Proliferation assay kit, Promega) reagent was added, and after incubation for 2 hours in a cell incubator, the degree of cell growth was measured by measuring absorbance at 490 nm using an ELISA reader.

The effect of human hair cell proliferation of the heat keeper extract is shown in FIG. 3, and the heat device extract showed a concentration-dependent effect on human hair cell proliferation.

Claims (5)

A composition for stimulating hair growth or inhibiting hair loss, comprising an extract from the heat group as an active ingredient.
According to claim 1,
The extract is a composition for promoting hair growth or inhibiting hair loss, characterized in that the water, lower alcohol having 1 to 4 carbon atoms, or a mixed solvent extract of the hot part.
According to claim 1 or 2,
The composition is a composition for promoting hair growth or inhibiting hair loss, characterized in that the food composition.
According to claim 1 or 2,
The composition is a composition for promoting hair growth or inhibiting hair loss, characterized in that the pharmaceutical composition.
According to claim 1 or 2,
The composition is a composition for promoting hair growth or inhibiting hair loss, characterized in that the hair cosmetic composition.

Images