KR20230069047A - 폴리뉴클레오티드를 증폭시키기 위한 조성물 및 방법 - Google Patents

폴리뉴클레오티드를 증폭시키기 위한 조성물 및 방법 Download PDF

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KR20230069047A
KR20230069047A KR1020227045843A KR20227045843A KR20230069047A KR 20230069047 A KR20230069047 A KR 20230069047A KR 1020227045843 A KR1020227045843 A KR 1020227045843A KR 20227045843 A KR20227045843 A KR 20227045843A KR 20230069047 A KR20230069047 A KR 20230069047A
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capture primers
orthogonal
capture
primers
region
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Korean (ko)
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제프리 피셔
제이슨 베틀리
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일루미나, 인코포레이티드
일루미나 케임브리지 리미티드
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/204—Modifications characterised by specific length of the oligonucleotides
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    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/513—Detection characterised by immobilisation to a surface characterised by the pattern of the arrayed oligonucleotides
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/543—Detection characterised by immobilisation to a surface characterised by the use of two or more capture oligonucleotide primers in concert, e.g. bridge amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
KR1020227045843A 2020-09-14 2021-08-27 폴리뉴클레오티드를 증폭시키기 위한 조성물 및 방법 Pending KR20230069047A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063077857P 2020-09-14 2020-09-14
US63/077,857 2020-09-14
PCT/US2021/048064 WO2022055729A1 (en) 2020-09-14 2021-08-27 Compositions and methods for amplifying polynucleotides

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KR20230069047A true KR20230069047A (ko) 2023-05-18

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KR1020227045843A Pending KR20230069047A (ko) 2020-09-14 2021-08-27 폴리뉴클레오티드를 증폭시키기 위한 조성물 및 방법

Country Status (14)

Country Link
US (2) US11913067B2 (https=)
EP (1) EP4211269B1 (https=)
JP (1) JP2023540422A (https=)
KR (1) KR20230069047A (https=)
CN (1) CN115777024A (https=)
AU (1) AU2021341688B2 (https=)
BR (1) BR112022026860A2 (https=)
CA (1) CA3182296A1 (https=)
ES (1) ES3007341T3 (https=)
IL (1) IL299327A (https=)
MX (1) MX2022014724A (https=)
TW (1) TW202225412A (https=)
WO (1) WO2022055729A1 (https=)
ZA (1) ZA202213367B (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022192083A1 (en) * 2021-03-09 2022-09-15 Illumina, Inc. Polymers, methods of making polymers, and methods of coupling oligonucleotides to polymers
US20250236865A1 (en) * 2021-10-20 2025-07-24 Illumina, Inc. Methods for capturing library dna for sequencing
US20240052406A1 (en) * 2022-08-04 2024-02-15 Singular Genomics Systems, Inc. Competitive methods and compositions for amplifying polynucleotides
EP4372101A1 (en) * 2022-11-02 2024-05-22 EsBiolab LLC Methods and devices of generating clusters of amplicons
WO2024216033A1 (en) * 2023-04-14 2024-10-17 Illumina, Inc. Capturing and amplifying polynucleotides

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0514909D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods of nucleic acid amplification and sequencing
PT2669387T (pt) * 2009-08-25 2016-09-20 Illumina Inc Métodos para selecção e amplificação de polinucleótidos
US9029103B2 (en) * 2010-08-27 2015-05-12 Illumina Cambridge Limited Methods for sequencing polynucleotides
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
EP2840148B1 (en) * 2013-08-23 2019-04-03 F. Hoffmann-La Roche AG Methods for nucleic acid amplification
GB201410646D0 (en) 2014-06-14 2014-07-30 Illumina Cambridge Ltd Methods of increasing sequencing accuracy
PT3218511T (pt) * 2014-11-11 2020-07-23 Illumina Cambridge Ltd Métodos e dispositivos para produção e sequenciamento de grupos monoclonais de ácido nucleico
DK3234187T3 (da) * 2014-12-15 2021-05-25 Illumina Inc Fremgangsmåde til enkeltmolekyleanbringelse på et substrat
ITUA20162640A1 (it) * 2016-04-15 2017-10-15 Menarini Silicon Biosystems Spa Metodo e kit per la generazione di librerie di dna per sequenziamento massivo parallelo
WO2018071522A1 (en) * 2016-10-11 2018-04-19 Life Technologies Corporation Rapid amplification of nucleic acids
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
AU2019368024B2 (en) * 2018-10-24 2026-02-12 University Of Washington Methods and kits for depletion and enrichment of nucleic acid sequences
DK3899037T3 (da) * 2018-12-19 2023-11-06 Illumina Inc Fremgangsmåder til forbedring af polynukleotidklyngeklonalitetsprioritet
WO2021173666A1 (en) * 2020-02-26 2021-09-02 Illumina, Inc. Kits for genotyping
PL3969618T3 (pl) * 2020-03-09 2024-01-03 Illumina, Inc. Sposoby sekwencjonowania polinukleotydów

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Publication number Publication date
ES3007341T3 (en) 2025-03-19
AU2021341688B2 (en) 2025-09-18
MX2022014724A (es) 2023-03-06
EP4211269A1 (en) 2023-07-19
US20240240243A1 (en) 2024-07-18
US20220090187A1 (en) 2022-03-24
IL299327A (en) 2023-02-01
TW202225412A (zh) 2022-07-01
US11913067B2 (en) 2024-02-27
EP4211269B1 (en) 2024-11-06
ZA202213367B (en) 2025-03-26
AU2021341688A1 (en) 2023-01-05
WO2022055729A1 (en) 2022-03-17
BR112022026860A2 (pt) 2023-04-11
JP2023540422A (ja) 2023-09-25
CA3182296A1 (en) 2022-03-17
CN115777024A (zh) 2023-03-10

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