KR20230064260A - Composition for preventing or treating muscular disease comprising syneilesis aconitifolia (bunge) maxim extract - Google Patents
Composition for preventing or treating muscular disease comprising syneilesis aconitifolia (bunge) maxim extract Download PDFInfo
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Abstract
Description
본 발명은 애기우산나물 추출물을 유효성분으로 포함하는 근육질환의 예방 또는 치료용 조성물에 관한 것이다. 보다 상세하게는 애기우산나물 추출물을 유효성분으로 포함하여 근 감소와 관련한 파골세포의 분화를 저하하고 근 섬유 형성을 촉진함으로써 근육량을 증가 효과를 갖는 동시에 복용량 및 복용기간이 증가하여도 부작용의 문제가 없는 근육질환의 예방 또는 치료용 조성물에 관한 것이다.The present invention relates to a composition for preventing or treating muscle diseases comprising an extract of Arabidopsis japonica as an active ingredient. More specifically, it contains Arabidopsis extract as an active ingredient to reduce the differentiation of osteoclasts related to muscle loss and promote muscle fiber formation, thereby increasing muscle mass and eliminating side effects even when the dose and duration of administration are increased. It relates to a composition for preventing or treating muscle diseases without.
근육(Muscle)이란 중배엽의 줄기세포가 발달되어 생성된 조직으로써 근육세포(myoblast)들의 결합으로 이루어진 근섬유(myofiber) 다발로 구성되어 있다. 근육은 체중의 40~50%를 차지하고 있어, 뼈와 내장 기관을 지탱 및 보호하는 동시에 심장의 박동과 같은 근육 외 다른 조직의 운동성을 갖도록 한다. 또한 기관의 보호 외에도 근육은 운동을 포함한 신체활동뿐만 아니라 영양소 대사에도 큰 영향을 주기 때문에 당뇨, 비만, 심혈관 질환 등과 같은 대사성 질환의 발병과도 밀접하게 연관되어 있다.Muscle is a tissue created by the development of mesodermal stem cells, and is composed of a myofiber bundle made of a combination of myoblasts. Muscles account for 40 to 50% of body weight, supporting and protecting bones and internal organs, and at the same time enabling movement of tissues other than muscles, such as heartbeat. In addition to organ protection, muscle has a great influence on nutrient metabolism as well as physical activity including exercise, so it is closely related to the occurrence of metabolic diseases such as diabetes, obesity, and cardiovascular disease.
사람의 근육은 40세 이후부터 매년 1% 이상씩 감소하며, 80세가 되면 최대 근육량의 50% 수준이 감소되어, 노년의 근육 감소는 전반적인 신체기능을 떨어뜨리는 가장 중요한 요소로 인식되어지고 있다. 때문에 근 기능을 개선하기 위한 필요성이 높아지고 있으며, 근육 기능 저하와 관련된 질병의 치료제 개발 또한 중요해지고 있다.A person's muscles decrease by more than 1% every year after the age of 40, and at the age of 80, 50% of the maximum muscle mass decreases, and muscle loss in old age is recognized as the most important factor in deteriorating overall body function. Therefore, the need to improve muscle function is increasing, and the development of treatments for diseases related to muscle function decline is also becoming important.
근 기능을 결정짓는 가장 중요한 요소는 근육량이며, 이는 단백질 합성과 분해의 균형에 의해 유지된다. 근 단백질 합성에 관여하는 인자들은 근 세포 내에서 PI3K (phosphatidylinositol-3 kinase/Akt pathway 경로의 자극을 기점으로 다운스트림 단백질을 인산화시킴으로써 단백질 합성을 유도한다. PI3K/Akt 신호전달에 의한 mTOR(mammalian target of rapamycin)의 활성은 세포 내에서 다양한 성장 신호를 통합하는 중심성장 신호전달 인자로 인정되고 있다. mTOR의 활성화는 두개의 다운스트림 타겟인 4E-BP1(4E-binding protein)과 p70S6K(phosphorylated 70-kDa ribosomal S6 kinase)를 활성화함으로써 근 단백질 합성을 유도하여 근육량 증가에 기여한다. 이러한 근 단백질 합성의 증가는 단백질의 증가에 따른 근육 크기 증가(hypertrophy, 근비대)나 근섬유 수 증가(hyperplasia)로 나타난다.The most important factor determining muscle function is muscle mass, which is maintained by the balance between protein synthesis and breakdown. Factors involved in muscle protein synthesis induce protein synthesis by phosphorylating downstream proteins starting from stimulation of the PI3K (phosphatidylinositol-3 kinase/Akt pathway) in muscle cells. mTOR (mammalian target) by PI3K/Akt signaling of rapamycin) is recognized as a central growth signaling factor that integrates various growth signals within the cell.Activation of mTOR activates two downstream targets, 4E-BP1 (4E-binding protein) and p70S6K (phosphorylated 70- kDa ribosomal S6 kinase) contributes to the increase in muscle mass by inducing muscle protein synthesis, which is manifested as an increase in muscle size (hypertrophy) or an increase in the number of muscle fibers (hyperplasia) due to an increase in protein.
반대로 전사 인자(transcription factor)인 FoxO(forhead box)가 세포질에서 핵 내로 이동하면 단백질 분해에 관여하는 E3 유비쿼틴 리가아제 인자인 아트로긴-1(atrogin-1)과 MuRF-1의 발현을 증가시키는데, 이 경우 근육 내의 단백질 분해가 촉진되어 근육량이 줄어들게 된다. 즉, 단백질 분해가 합성보다 더 일어나게 되는 것으로 근육량 감소에 따른 근육의 약화 및 퇴행을 일으키게 된다.Conversely, when the transcription factor FoxO (forhead box) moves from the cytoplasm to the nucleus, it increases the expression of atrogin-1 and MuRF-1, which are E3 ubiquitin ligase factors involved in protein degradation. , In this case, protein breakdown within the muscle is promoted, resulting in a decrease in muscle mass. In other words, protein degradation occurs more than synthesis, which causes muscle weakness and degeneration due to a decrease in muscle mass.
따라서 mTOR의 활성 촉진과 아트로긴-1(atrogin-1)과 MuRF-1의 발현 억제는 근육 단백질의 양을 증가시켜 근육량을 증가시키게 되므로, mTOR과 아트로긴-1(atrogin-1), MuRF-1의 발현은 근육량 조절에 중요한 요소가 된다.Therefore, promotion of mTOR activity and suppression of expression of atrogin-1 and MuRF-1 increase the amount of muscle protein and thus increase muscle mass. 1 expression is an important factor in muscle mass control.
이에 근육 세포에서 근단백질 합성 및 근육량 증가와 관련된 단백질의 발현을 증가시키고, 근육량 조절을 통해 근육의 약화 및 퇴행을 예방 및 개선할 수 있는 물질의 개발이 필요한 실정이다.Therefore, it is necessary to develop a substance capable of increasing the expression of proteins related to muscle protein synthesis and muscle mass increase in muscle cells, and preventing and improving muscle weakness and degeneration through muscle mass control.
본 발명의 목적은 애기우산나물 추출물을 포함하는 근육질환의 예방 또는 치료용 조성물을 제공하는 것이다.An object of the present invention is to provide a composition for preventing or treating muscle diseases containing an extract of Arabidopsis.
본 발명의 다른 목적은 근 감소와 관련한 파골세포의 분화를 저하하고 근 섬유 형성을 촉진함으로써 근육량을 증가 효과를 갖는 천연물 유래의 근육질환의 예방 또는 치료용 조성물을 제공하는 것이다.Another object of the present invention is to provide a composition for preventing or treating muscle diseases derived from natural products having an effect of increasing muscle mass by reducing the differentiation of osteoclasts associated with muscle loss and promoting muscle fiber formation.
본 발명의 다른 목적은 부작용의 문제가 없으며, 주기적 또는 장기적으로 사용할 수 있는 근육질환의 예방 또는 치료용 조성물을 제공하는 것이다.Another object of the present invention is to provide a composition for preventing or treating muscle diseases that has no side effects and can be used periodically or for a long period of time.
상기 목적을 달성하기 위하여, 본 발명의 일 실시예에 따른 근육질환의 예방 또는 치료용 조성물은 애기우산나물(Syneilesis aconitifolia (Bunge) Maxim) 추출물을 유효성분으로 포함한다.In order to achieve the above object, the composition for preventing or treating muscle diseases according to an embodiment of the present invention includes Syneilesis aconitifolia (Bunge) Maxim extract as an active ingredient.
상기 추출물은 물, C1 내지 C6의 저급 알코올 및 이들의 혼합물로 이루어진 군으로부터 선택된 추출 용매를 이용하여 추출되는 것이다.The extract is extracted using an extraction solvent selected from the group consisting of water, C 1 to C 6 lower alcohol, and mixtures thereof.
상기 근육질환은 긴장감퇴증(atony), 근위축증(muscular atrophy), 근이영양증(muscular dystrophy), 근무력증, 악액질(cachexia), 경직성 척추 증후군(rigid spinesyndrome), 근위축성 측삭경화증(amyotrophic lateral sclerosis), 샤르코-마리-투스병(Charcot-Marie-Tooth disease) 및 근육 감소증(sarcopenia)으로 이루어진 군으로부터 선택되는 어느 하나 이상인 것을 특징으로 한다.The muscle disease is atony, muscular atrophy, muscular dystrophy, myasthenia, cachexia, rigid spine syndrome, amyotrophic lateral sclerosis, Charcot- It is characterized by at least one selected from the group consisting of Charcot-Marie-Tooth disease and sarcopenia.
상기 조성물은 파골세포의 분화 억제능을 나타내는 것이다.The composition exhibits the ability to inhibit the differentiation of osteoclasts.
상기 조성물은 근 섬유 형성 촉진 효과를 통해 근육량(muscle mass)을 증가시키는 것이다.The composition is to increase muscle mass (muscle mass) through the effect of promoting muscle fiber formation.
본 발명의 다른 일 실시예에 따른 약학 조성물은 상기 조성물을 포함하는 것이다.A pharmaceutical composition according to another embodiment of the present invention is to include the above composition.
본 발명의 다른 일 실시예에 따른 식품 조성물은 상기 조성물을 포함하는 것이다.A food composition according to another embodiment of the present invention includes the above composition.
이하, 본 발명을 더욱 상세하게 설명한다.Hereinafter, the present invention will be described in more detail.
본 명세서에서 사용되는 용어 "추출물"은 상술한 바와 같이 당업계에서 조추출물(crude extract)로 통용되는 의미를 갖지만, 광의적으로는 추출물을 추가적으로 분획(fractionation)한 분획물도 포함한다. 즉, 추출물은 상술한 추출 용매를 이용하여 얻은 것뿐만 아니라, 여기에 정제과정을 추가적으로 적용하여 얻은 것도 포함한다. 예컨대, 상기 추출물을 일정한 분자량 컷-오프 값을 갖는 한외여과막을 통과시켜 얻은 분획, 다양한 크로마토그래피(크기, 전하, 소수성 또는 친화성에 따른 분리를 위해 제작된 것)에 의한 분리 등, 추가적으로 실시된 다양한 정제 방법을 통해 얻어진 분획도 본 발명의 추출물에 포함되는 것이다.As used herein, the term "extract" has a meaning commonly used in the art as a crude extract in the art as described above, but also includes fractions obtained by additional fractionation of the extract in a broad sense. That is, the extract includes not only those obtained using the above-described extraction solvent, but also those obtained by additionally applying a purification process thereto. For example, a fraction obtained by passing the extract through an ultrafiltration membrane having a certain molecular weight cut-off value, separation by various chromatography (made for separation according to size, charge, hydrophobicity or affinity), etc. Fractions obtained through the purification method are also included in the extract of the present invention.
본 발명의 명세서에서 용어 "유효성분으로 포함"은 질병 또는 장애의 징후 또는 증상의 예방, 감소, 경감 또는 제거를 포함하나, 제한되지 않는 유익한 결과와 같은, 바람직한 생물학적 효과에 영향을 미치기에 충분한 양을 포함하는 것을 의미하고, 이 양을 “유효량”이라 한다. 따라서, 조성물 또는 방법의 각 활성 성분의 총량은 유의미한 개체의 이익을 나타내기에 충분하다. 따라서, 유효량은 투여되는 상황에 따라 달라질 것이다. As used herein, the term "comprising as an active ingredient" refers to an amount sufficient to affect a desired biological effect, such as beneficial results, including but not limited to prevention, reduction, alleviation or elimination of signs or symptoms of a disease or disorder. , and this amount is referred to as the “effective amount”. Thus, the total amount of each active ingredient in the composition or method is sufficient to yield a significant individual benefit. Thus, the effective amount will depend on the circumstances in which it is being administered.
본 명세서에서 사용되는 용어 중, 어떤 부분이 어떤 구성요소를 "포함"한다고 할 때, 이는 특별히 반대되는 기재가 없는 한 다른 구성요소를 제외하는 것이 아니라 다른 구성 요소를 더 포함할 수 있는 것을 의미한다.Among the terms used in this specification, when a certain component is said to "include", it means that it may further include other components, not excluding other components unless otherwise stated. .
본 발명에서 사용되는 용어 "개선"은 상태의 완화 또는 치료와 관련된 파라미터, 예를 들면 증상의 정도를 적어도 감소시키는 모든 행위를 의미한다.As used herein, the term "improvement" refers to any activity that at least reduces a parameter associated with alleviation or treatment of a condition, for example, the severity of a symptom.
본 명세서의 용어 "예방"은, 예를 들어, 질환 원인에의 노출에 의해, 질병이 생길 위험이 있는 무증상 대상체에서, 질병 또는 질병과 관련된 증상의 출현을 차단함을 지칭한다.As used herein, the term “prevention” refers to preventing the appearance of a disease or symptoms associated with a disease in asymptomatic subjects at risk of developing the disease, for example, by exposure to the cause of the disease.
본 명세서의 용어 "치료"는 발병된 후에 질병 또는 병리학적 상태의 징후 또는 증상을 개선하는 치료적 개재를 지칭한다.The term "treatment" as used herein refers to therapeutic intervention that ameliorates the signs or symptoms of a disease or pathological condition after it has developed.
본 발명의 일 실시예에 따른 근육질환의 예방 또는 치료용 조성물은 애기우산나물(Syneilesis aconitifolia (Bunge) Maxim) 추출물을 유효성분으로 포함한다.A composition for preventing or treating muscle diseases according to an embodiment of the present invention includes an extract of Syneilesis aconitifolia (Bunge) Maxim as an active ingredient.
애기우산나물 추출물(Syneilesis aconitifolia (Bunge) Maxim)은 국화과의 다년초로 높이 70-120cm이고, 뿌리는 짧은 근경이 옆으로 뻗으며, 원줄기는 자주 빛이 돌고 가지가 없는데 2개의 잎이 달린다. 첫째 잎은 둥글고 지름 20-30cm로서 장상으로 갈라지며, 열편은 7-9개이고 2-3회 2개씩 중열된다. 둘째 잎은 약간 작으며 지름 12-24cm이고, 열편은 폭 4-5cm치며 엽병은 길이 2-6cm이다잎은 둥글고 지름 20~ 30cm로서 장상으로 갈라지며 꽃은 7월에서 8월에 핀다.Syneilesis aconitifolia (Bunge) Maxim is a perennial plant of the Asteraceae family with a height of 70-120cm. The first leaf is round, 20-30cm in diameter, split into an oblong shape, and has 7-9 lobes, 2-3 times in rows. The second leaf is slightly smaller, 12-24cm in diameter, the lobe is 4-5cm wide, and the petiole is 2-6cm long.
상기 애기우산나물 추출물은 물, C1 내지 C6의 저급 알코올 및 이들의 혼합물로 이루어진 군으로부터 선택된 추출 용매를 이용하여 추출되는 것이다.The Arabidopsis extract is extracted using an extraction solvent selected from the group consisting of water, C 1 to C 6 lower alcohols, and mixtures thereof.
구체적으로, 애기우산나물 추출물을 제조하기 위해서는 애기우산나물을 세척하는 단계; 세척 후 건조시키는 단계; 건조 후 애기우산나물을 분쇄하는 단계; 유기 용매를 사용하여 상기 분쇄물을 침출시키는 단계; 시료를 침출 후 건조시키는 단계; 물을 이용하여 침출시키는 단계; 및 침출하는 단계를 포함하여, 애기우산나물 추출물을 획득할 수 있다.Specifically, in order to prepare an extract of Arabidopsis herb, washing the Arabidopsis herb; drying after washing; Grinding Arabidopsis after drying; leaching the pulverized product using an organic solvent; drying the sample after leaching; leaching with water; And including the step of leaching, it is possible to obtain a Arabidopsis extract.
상기 유기 용매를 사용하여 추출한 추출물은 유기 용매를 사용하여 분획을 실시하는 단계를 더 포함할 수 있으며, 상기 추출물을 제조하는 방법은 초음파 추출법, 침출법 및 환류 추출법 등 당업계의 통상적인 추출 방법일 수 있다. The extract extracted using the organic solvent may further include fractionation using an organic solvent, and the method for preparing the extract may be a conventional extraction method in the art such as ultrasonic extraction, leaching, and reflux extraction. can
구체적으로 세척 및 건조로 이물질이 제거된 애기우산나물 추출물을 물, 탄소수 1 내지 6의 알코올 또는 이들의 혼합 용매로 추출한 추출물일 수 있으며, 상기 용매들을 순차적으로 시료에 적용하여 추출한 추출물일 수 있다.Specifically, the Arabidopsis extract, from which foreign substances are removed by washing and drying, may be an extract extracted with water, alcohol having 1 to 6 carbon atoms, or a mixed solvent thereof, and may be an extract extracted by sequentially applying the solvents to the sample.
상기 환류 추출법은 물, 탄소수 1 내지 6의 알코올 100 mL기준으로, 천연물의 분쇄물 10 내지 30g, 환류 시간 1 내지 3시간 및 50 내지 100%의 탄소수 1 내지 6의 알코올 또는 물에 의한다. 보다 구체적으로, 탄소수 1 내지 6의 알코올 100 mL 또는 물 100 mL 기준으로, 천연물의 분쇄물 10 내지 20g, 환류 시간 1 내지 2시간 및 70 내지 90%의 탄소수 1 내지 4의 알코올 또는 물에 의한 것이다.The reflux extraction method is based on 100 mL of water and alcohol having 1 to 6 carbon atoms, 10 to 30 g of pulverized natural product, 1 to 3 hours of reflux time, and 50 to 100% of alcohol having 1 to 6 carbon atoms or water. More specifically, based on 100 mL of alcohol having 1 to 6 carbon atoms or 100 mL of water, 10 to 20 g of pulverized natural product, 1 to 2 hours of reflux, and 70 to 90% of alcohol or water having 1 to 4 carbon atoms .
상기 침출법은 15 내지 30℃, 24 내지 72시간 동안 진행하며, 추출 용매로 물 또는 50 내지 100%의 탄소수 1 내지 6의 알코올을 이용한다. 보다 구체적으로는 20 내지 25℃, 30 내지 54시간 동안 진행하며, 추출 용매는 물 또는 70 내지 80%의 탄소수 1 내지 6의 알코올에 의한 것이다.The leaching method is carried out at 15 to 30 ° C. for 24 to 72 hours, and water or 50 to 100% of alcohol having 1 to 6 carbon atoms is used as an extraction solvent. More specifically, it is performed at 20 to 25 ° C. for 30 to 54 hours, and the extraction solvent is water or 70 to 80% of alcohol having 1 to 6 carbon atoms.
상기 초음파 추출법은 30 내지 50℃, 0.5 내지 2.5시간 동안 반응을 진행하며, 추출용매는 물 또는 50 내지 100%의 탄소수 1 내지 6의 알코올에 의한 것이다. 구체적으로는 40 내지 50℃, 1 내지 2.5시간 동안 추출하며, 추출용매로 물 또는 70 내지 80%의 탄소수 1 내지 6의 알코올에 의한 것이다.In the ultrasonic extraction method, the reaction is performed at 30 to 50 ° C. for 0.5 to 2.5 hours, and the extraction solvent is water or 50 to 100% of alcohol having 1 to 6 carbon atoms. Specifically, extraction is performed at 40 to 50 ° C. for 1 to 2.5 hours, and water or 70 to 80% of alcohol having 1 to 6 carbon atoms is used as an extraction solvent.
상기 추출 용매는 시료의 중량 기준으로 2 내지 50배를 사용할 수 있으며, 보다 구체적으로는 2 내지 20배이다. 추출을 위해 시료는 추출 용매에서 추출을 위해 1 내지 72시간 동안 방치될 수 있으며, 보다 구체적으로 24 내지 48시간 동안 방치될 수 있다.The extraction solvent may be used in an amount of 2 to 50 times, more specifically, 2 to 20 times, based on the weight of the sample. For extraction, the sample may be left for 1 to 72 hours for extraction in an extraction solvent, more specifically, for 24 to 48 hours.
추출 후, 추출물은 새로운 분획 용매를 순차적으로 적용하여 분획할 수 있다. 분획 시 사용하는 분획 용매는 상기 용매는 물, 헥산, 부탄올, 에틸아세트산, 에틸 아세테이트, 메틸렌클로라이드 및 이들의 혼합물로 이루어진 군으로부터 선택된 어느 하나 이상이며, 바람직하게는 에틸아세테이트 또는 메틸렌클로라이드이다. 추출물 또는 분획물을 얻은 후에는 농축 또는 동결건조 등의 방법을 추가적으로 사용할 수 있다.After extraction, the extract can be fractionated by sequentially applying a fresh fractionation solvent. The fractionation solvent used in the fractionation is at least one selected from the group consisting of water, hexane, butanol, ethylacetic acid, ethyl acetate, methylene chloride, and mixtures thereof, and is preferably ethyl acetate or methylene chloride. After obtaining the extract or fraction, a method such as concentration or lyophilization may be additionally used.
상기 근육질환은 긴장감퇴증(atony), 근위축증(muscular atrophy), 근이영양증(muscular dystrophy), 근무력증, 악액질(cachexia), 경직성 척추 증후군(rigid spinesyndrome), 근위축성 측삭경화증(amyotrophic lateral sclerosis), 샤르코-마리-투스병(Charcot-Marie-Tooth disease) 및 근육 감소증(sarcopenia)으로 이루어진 군으로부터 선택되는 어느 하나 이상인 것을 특징으로 한다.The muscle disease is atony, muscular atrophy, muscular dystrophy, myasthenia, cachexia, rigid spine syndrome, amyotrophic lateral sclerosis, Charcot- It is characterized by at least one selected from the group consisting of Charcot-Marie-Tooth disease and sarcopenia.
상기 긴장감퇴증은 근긴장의 감퇴 또는 소실한 상태를 말한다. 근긴장은 중추성, 반사성으로 조절되며, 이 조절기구의 장애에 따라서 근긴장의 증가, 감퇴ㆍ소실을 볼 수 있다. 또한 자율신경기관(심장ㆍ위ㆍ장)의 근이 긴장의 저하ㆍ소실을 가져왔을 때에도 이 용어가 사용된다.The hypotonia refers to a state in which muscle tone is reduced or lost. Muscle tone is regulated centrally and reflexively, and depending on the disorder of this control mechanism, muscle tone may increase, decrease, or disappear. Also, this term is used when the muscles of the autonomic nervous system (heart, stomach, intestines) cause a decrease or loss of tension.
근위축증은 사지의 근육이 거의 좌우대칭적으로 점점 위축되어 가는 것으로 여러 형태가 있다. 가장 많은 것은 근위축성 측삭경화증(側索硬化症)과 척수성진행성 근위축증이다. 다같이 척수에 있는 운동신경섬유 및 세포의 진행성 변성에 의한 것이지만 원인은 불명하다. 근위축성 측삭경화증은 19세기 말 유럽에서 처음으로 보고된 이래 원인이 아직 밝혀져 있지 않은 불치병이다. 영국의 천문학자 스티븐 호킹이 이 병을 앓고 있어잘 알려져 있으며, 미국 메이저리그 L.게릭이 이 병으로 사망하였다고 하여 ‘루게릭병’이라고도 부른다.Muscular atrophy is a condition in which the muscles of the limbs gradually atrophy almost bilaterally, and there are several forms. The most common are amyotrophic lateral sclerosis and spinal cord progressive muscular atrophy. Both are caused by progressive degeneration of motor nerve fibers and cells in the spinal cord, but the cause is unknown. Amyotrophic lateral sclerosis is an incurable disease whose cause has not yet been identified since it was first reported in Europe at the end of the 19th century. It is well known that British astronomer Stephen Hawking suffered from this disease, and it is also called ‘Lou Gehrig’s disease’ because American major leaguer L. Gehrig died of this disease.
근이영양증은 중추신경계와 말초신경계와는 연관 없이 골격근의 퇴화가 진행되어 근육의 약화, 구축, 변형을 보이며 특정 근육에 가성대비나 진행성으로 오는 대칭성 근위축이 나타나며, 근이영양증은 근육 약화의 정도와 유전적 패턴에 따라 몇 가지 형태로 분류된다. Muscular dystrophy is a degeneration of skeletal muscle that is not related to the central nervous system and the peripheral nervous system, showing muscle weakness, contracture, and deformation, and symmetrical muscular atrophy that comes in pseudo-contrast or progressive in a specific muscle. It is classified into several types according to the pattern.
근이영양증은 근위축증과 구별 없이 사용되기도 하지만 임상적 증상에는 약간의 차이가 있다. 근이영양증은 주로 유년기에 발생하고 근위축증은 청년기에 발생한다. 또한 근이영양증은 근위부 근육에, 근위축증은 윈위부 근육에 발생한다. 근강직이 근이영양증에는 없고 근위축증에는 있으며, 근이영양증은 유전성이 확실하지만 근위축은 유전적 경향이 드물다. Muscular dystrophy is sometimes used interchangeably with muscular dystrophy, but there are slight differences in clinical symptoms. Muscular dystrophy usually occurs in childhood, and muscular dystrophy occurs in adolescence. In addition, muscular dystrophy occurs in the proximal muscle, and muscular dystrophy occurs in the distal muscle. Muscular rigidity does not exist in muscular dystrophy, but is present in muscular dystrophy, and muscular dystrophy is heritable, but the heritability of muscular dystrophy is rare.
상기 조성물은 파골세포의 분화 억제능을 나타내는 것이다.The composition exhibits the ability to inhibit the differentiation of osteoclasts.
근감소증 또는 골다공증의 원인이 되는 근육 감소 및 파골세포는 뼈 조직을 파괴하는 세포로, 뼈 조직을 만드는 세포인 조골세포와 균형이 잘 이루어져야 건강한 뼈를 유지할 수 있다. Muscle loss and osteoclasts, which cause sarcopenia or osteoporosis, are cells that destroy bone tissue, and healthy bones can be maintained only when they are well balanced with osteoblasts, which are cells that make bone tissue.
따라서, 본 발명의 근육질환 예방 및 치료용 조성물은 파골세포의 분화를 효과적으로 억제할 수 있는 것으로, 조골세포와의 균형을 이루며, 파골세포의 과다 분화로 인해 발생하는 질병 또는 질환을 예방 또는 치료할 수 있다.Therefore, the composition for preventing and treating muscle diseases of the present invention can effectively inhibit the differentiation of osteoclasts, achieve a balance with osteoblasts, and prevent or treat diseases or disorders caused by excessive differentiation of osteoclasts. there is.
상기 조성물은 근 섬유 형성 촉진 효과를 통해 근육량(muscle mass)을 증가시키는 것이다.The composition is to increase muscle mass (muscle mass) through the effect of promoting muscle fiber formation.
근육의 성장은 개개의 근모세포간의 결합에 다른 근 섬유 형성에 기인함에 따라, 본 발명의 근육질환 예방 및 치료용 조성물은 근 섬유 형성 촉진 효과를 통해, 근육량을 증가시켜, 근육의 성장을 나타내는 것이다.As muscle growth is due to the formation of muscle fibers different from the binding between individual myoblast cells, the composition for preventing and treating muscle diseases of the present invention increases muscle mass through the effect of promoting muscle fiber formation, thereby showing muscle growth. .
바람직하게는 본 발명의 근육질환의 예방 및 치료용 조성물은 덜꿩나무(Viburnum erosum Thunb.) 추출물, 파드득나물(Cryptotaenia japonica) 추출물, 털중나리(Lilium amabile) 추출물 및 이들의 혼합으로 이루어진 군으로부터 선택되는 천연 추출물을 추가로 포함할 수 있다.Preferably, the composition for preventing and treating muscle diseases of the present invention is selected from the group consisting of Viburnum erosum Thunb. extract, Cryptotaenia japonica extract, Lilium amabile extract, and mixtures thereof Natural extracts may further be included.
상기 덜꿩나무(Viburnum erosum Thunb.)는 인동과 덜꿩나무속에 속하는 낙엽 활엽 관목으로, 경기도 이남의 낮은 산지에서 흔하게 볼 수 있는 나무이다. 인동과의 키작은 나무로 높이는 2 내지 3m이다. 봄철의 하얀색 꽃과 가을철의 붉은 열매가 아름다워 공원수로 많이 식재된다. 잎은 타원형으로 마주보기로 달리는 것이 특징이다. 앞, 뒷면에 털이 소복하게 나 있어 만지면 부드러운 느낌을 준다. 줄기는 여러 개로 갈라져 포기를 이루어 자라는 것이 특징이며, 꽃은 4 내지 5월에 새로 나온 가지 끝에 하얀색 양성화가 동그란 모양을 만들며 모여 핀다. 팥처럼 생긴 조그만 열매는 9 내지 10월에 붉게 익는데 새들의 좋은 먹이가 된다. 햇볕이 적당히 드는 숲에서 자라며, 보습성과 배수성이 좋은 토양을 좋아한다. 추위를 견디는 내한성이 매우 강하고 양지와 음지에서 모두 잘 자라며, 건조 환경에서도 잘 자라는 특성을 갖는다. 한편, 덜꿩나무라는 이름은 꿩과 관련이 있는 것으로 보이며. 구체적으로, 들에 있는 꿩들이 좋아하는 열매를 달고 있다는 뜻으로 들꿩나무로 불리다가 덜꿩나무가 된 것으로 추정된다. 어린 잎은 나물로 먹을 수 있고, 늙은 잎과 줄기는 한방에서 구내염이나 가려움증의 약재로 사용하는 것이 특징이다.The lesser pheasant tree (Viburnum erosum Thunb.) is a deciduous broad-leaved shrub belonging to the genus Indong and lesser pheasant, and is a tree commonly found in low mountains in the south of Gyeonggi-do. It is a short tree of the honeysuckle family and is 2 to 3 m high. It is often planted as a park tree because of its beautiful white flowers in spring and red fruits in autumn. The leaves are elliptical and are characterized by running oppositely. The hair on the front and back gives a soft feel to the touch. Its characteristic is that the stem splits into several pieces to form a branch, and the white bisexual flowers form a round shape at the end of the newly emerged branch in April or May. The small red bean-like fruits ripen red in September or October and are good food for birds. It grows in moderately sunny forests and likes moist and well-drained soil. It has very strong resistance to cold, grows well in both sunny and shady areas, and grows well in a dry environment. On the other hand, the name of the lesser pheasant tree seems to be related to the pheasant. Specifically, it is presumed that it was called the Peel Pheasant Tree, which means that the pheasants in the field have a favorite fruit, and then became the Leor Pheasant Tree. Young leaves can be eaten as herbs, and old leaves and stems are characterized by being used as a medicine for stomatitis or itching in oriental medicine.
상기 파드득나물(Cryptotaenia japonica)은 반디나물이라고도 일컬어지는 우리나라 산지에서 자라는 나물이다. 높이는 약 30 내지 60cm이다. 줄기는 곧추서고 약간 갈라지며 전체에 털이 없고 향기가 있다. 줄기와 잎은 녹색이고 뿌리는 굵은 것이 특징이다. 잎은 어긋나고 잎자루가 5 내지 17cm로 길며 3 개의 작은 잎으로 되는데 뒷면에 윤기가 있는 것이 특징이다. 한편, 끝쪽의 작은 잎은 잎자루가 없고 달걀 모양으로서 길이 3 내지 8cm이며, 나비 2 내지 6cm이다. 끝이 뾰족하고 밑부분은 급히 좁아지며 가장 자리에 날카롭고 불규칙한 톱니가 있다. 곁쪽의 작은 잎은 크기와 모양이 서로 같고 잎자루가 없으며 때로 불규칙한 톱니가 있는 가장자리가 2 내지 3개로 얕게 갈라진다. 꽃은 흰색 또는 연한 자주색이며 6 내지 7월에 가지 끝에 복산형꽃차례[複傘形花序]로 달린다. 작은 꽃자루는 1 내지 4개로서 길고 짧은 것이 있는데 길이 3 내지 15mm이고 곧추서는 특징을 갖는다. 작은 총포조각은 줄 모양이고 짧다. 꽃잎은 5개로서 끝부분이 안으로 굽고 꽃받침은 5개이며 수술도 5개이다. 열매는 분열과로서 길이 3 내지 4mm의 긴 타원형이며, 털이 없고 8 내지 9월에 익는 것이 특징이다. 어린 잎은 식용하고 야채로 재배하기도 한다. 여름에 채취한 것은 그늘에 말려서 갑상선종 등에 약재로 이용하기도 한다.The Padduk herb (Cryptotaenia japonica) is an herb that grows in the mountainous regions of Korea, also known as Bandinamul. The height is about 30 to 60 cm. The stem is upright, slightly split, hairless, and fragrant. The stem and leaves are green and the root is thick. The leaves are alternate, the petiole is 5 to 17 cm long, and there are 3 small leaves, which are characterized by the glossy back side. On the other hand, the small leaf at the end has no petiole, is egg-shaped, and is 3 to 8 cm long and 2 to 6 cm wide. The tip is pointed, the lower part narrows rapidly, and there are sharp and irregular teeth at the edge. Side small leaves are the same size and shape, have no petioles, and sometimes have irregularly sawtoothed edges that are shallowly split into 2 or 3 pieces. Flowers are white or light purple, and hang in a double inflorescence [複傘形花序] at the end of branches in June or July. There are 1 to 4 small peduncles, long and short ones, 3 to 15mm long, and stand upright. Small gun pieces are line-shaped and short. There are 5 petals, the ends of which are bent inward, and there are 5 calyxes and 5 stamens. The fruit is a divisional fruit with a long oval shape of 3 to 4 mm in length, and is characterized by being hairless and ripening in August to September. The young leaves are edible and are also grown as vegetables. It is collected in summer and dried in the shade to be used as a medicine for goiter.
상기 털중나리(Lilium amabile)는 외떡잎식물 백합목 백합과의 여러해살이풀로 우리나라 산지에서 흔히 자란다. 높이가 50 내지 100cm까지 자라며, 줄기는 곧추서고 윗부분이 약간 갈라지며 전체에 잿빛의 잔털이 난다. 비늘줄기는 길이 2 내지 4cm, 지름 15 내지 25mm로 달걀 모양 타원형이다. 잎은 어긋나고 줄 모양이거나 바소꼴이며 길이 3 내지 7cm, 나비 3 내지 8mm이다. 둔한 녹색이고 끝이 뭉뚝하거나 뾰족하며 양면에 잔털이 빽빽이 난다. 가장자리는 밋밋하고 잎자루가 없으며 위쪽으로 갈수록 크기가 작아지는 특징을 나타낸다. 한편, 꽃은 6 내지 8월에 피는데, 가지 끝과 원줄기 끝에 1 내지 5개씩 밑을 향하여 달린다. 화피갈래조각은 바소꼴이고 6개이며, 길이 4 내지 7cm, 나비 10 내지 15mm이다. 뒤쪽으로 젖혀지고, 안쪽에는 검은빛 또는 자줏빛 반점이 있다. 6 개의 수술과 1개의 암술은 모두 꽃 밖으로 길게 나온다. 꽃밥은 노란빛을 띈 빨간색이며, 길이는 약 10 내지 13mm이다. 열매는 삭과로서 달걀 모양의 넓은 타원형이고 9 내지 10월에 익는다. 관상용으로 심으며, 이른 봄 비늘줄기를 식용하고 참나리와 함께 약재로도 쓰이는 특징을 갖는다.The hairy jungnari (Lilium amabile) is a perennial plant of the monocotyledonous lily lily family and commonly grows in the mountains of Korea. It grows up to 50 to 100 cm in height, the stem is upright, the upper part is slightly split, and gray fine hairs are present throughout. Scaly stems are 2 to 4 cm long and 15 to 25 mm in diameter, egg-shaped oval. Leaves are alternate, line-shaped or lanceolate, 3 to 7 cm long and 3 to 8 mm wide. It is dull green, the tip is blunt or pointed, and fine hairs grow densely on both sides. The edge is flat, there is no petiole, and the size decreases toward the top. On the other hand, flowers bloom in June to August, and 1 to 5 flowers run downward at the ends of branches and main stems. The perianth pieces are lanceolate, 6 in number, 4 to 7 cm long, and 10 to 15 mm wide. It is bent backwards, and there are black or purple spots on the inside. All 6 stamens and 1 pistil come out of the flower long. Anthers are yellowish red, about 10 to 13 mm long. Fruits are capsules, egg-shaped, wide oval, and ripen in September or October. It is planted for ornamental purposes, eats scale stems in early spring, and has the characteristics of being used as a medicinal material along with chamomile lily.
본 발명 조성물에 상기 천연 추출물이 추가로 포함되는 경우, 애기우산나물 추출물과 혼합에 따른 상승 효과로 조성물의 안정성을 극대화시킬 수 있으며, 파골세포 분화 억제능 및 근 섬유 형성 촉진 효과를 극대화시킬 수 있다. When the natural extract is further included in the composition of the present invention, the stability of the composition can be maximized due to the synergistic effect of mixing with the Arabidopsis extract, and the ability to inhibit osteoclast differentiation and promote muscle fiber formation can be maximized.
더 나아가, 상기 조성물을 건강기능식품 등으로 이용하는 경우 그 기호도 또한 극대화시킬 수 있는 장점이 있다.Furthermore, when the composition is used as a health functional food, etc., it has the advantage of maximizing its taste.
보다 바람직하게는 본 발명의 근육질환의 예방 또는 치료용 조성물은 애기우산나물 추출물 100 중량부에 대하여, 덜꿩나무 추출물 10 내지 20 중량부, 파드득나물 추출물 10 내지 20 중량부 및 털중나리 추출물 10 내지 20 중량부를 포함할 수 있다.More preferably, the composition for the prevention or treatment of muscle diseases of the present invention contains 10 to 20 parts by weight of chinensis extract, 10 to 20 parts by weight of Padduk herb extract, and 10 to 20 parts by weight of Euphorbia spp. It may contain parts by weight.
상기 중량 범위에 의하는 경우, 상기 파골세포 분화 억제능 및 근 섬유 형성 촉진 효과를 보다 극대화시킬 수 있을 뿐만 아니라, 기호도 또한 극대화시킬 수 있다.In the case of the above weight range, not only can the osteoclast differentiation inhibitory ability and the muscle fiber formation promoting effect be more maximized, but also the taste can be maximized.
본 발명의 다른 일 실시예에 따른 약학 조성물은 상기 조성물을 포함하여 제조된 것이다.A pharmaceutical composition according to another embodiment of the present invention is prepared by including the above composition.
상기 약학 조성물은, 각각 통상의 방법에 따라 산제, 과립제, 정제, 캡슐제, 현탁액, 에멀젼, 시럽, 에어로졸 등의 경구형 제형, 외용제, 좌제 및 멸균주사용액의 형태로 제형화하여 사용될 수 있다. The pharmaceutical composition may be formulated and used in the form of oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories and sterile injection solutions according to conventional methods, respectively.
경구 투여를 위한 고형제제에는 정제, 환제, 산제, 과립제, 캡슐제 등이 포함되며, 이러한 고형 제제는 상기 화합물은 적어도 하나 이상의 부형제, 예를 들면, 전분, 칼슘카보네이트(calcium carbonate), 수크로스(sucrose) 또는 락토오스(lactose), 젤라틴 등을 섞어 조제할 수 있다.Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and these solid preparations contain at least one excipient, for example, starch, calcium carbonate, sucrose ( It can be prepared by mixing sucrose, lactose, or gelatin.
또한, 단순한 부형제 이외에 마그네슘 스테아레이트, 탈크 같은 윤활제들도 사용된다. 경구를 위한 액상 제제로는 현탁제, 내용액제, 유제, 시럽제 등이 해당되는데 흔히 사용되는 단순 희석제인 물, 리퀴드 파라핀 이외에 여러 가지 부형제, 예를 들면 습윤제, 감미제, 방향제 및 보존제 등이 포함될 수 있다.In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid formulations for oral use include suspensions, solutions for oral use, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, sweeteners, aromatics and preservatives may be included. .
비경구 투여를 위한 제제에는 멸균된 수용액, 비수성 용제, 현탁제, 유제, 동결건조 제제, 좌제가 포함된다. 비수성 용제, 현탁제로는 프로필렌글리콜(propylene glycol), 폴리에틸렌 글리콜, 올리브 오일과 같은 식물성 기름, 에틸올 레이트와 같은 주사 가능한 에스테르 등이 사용될 수 있다.Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried formulations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used as non-aqueous solvents and suspending agents.
좌제의 기제로는 위텝솔(witepsol), 마크로골, 트윈(tween) 61, 카카오지, 라우린지, 글리세로제라틴 등이 사용될 수 있다.As a base for the suppository, witepsol, macrogol, tween 61, cacao butter, laurin paper, glycerogeratin and the like may be used.
본 발명의 약학적 조성물은 약제학적으로 허용 가능한 첨가제를 더 포함할 수 있으며, 이때 약제학적으로 허용 가능한 첨가제로는 전분, 젤라틴화 전분, 미결정셀룰로오스, 유당, 포비돈, 콜로이달실리콘디옥사이드, 인산수 소칼슘, 락토스, 만니톨, 엿, 아라비아고무, 전호화전분, 옥수수전분, 분말셀룰로오스, 히드록시프로필셀룰로오스, 오파드라이, 전분글리콜산나트륨, 카르나우바납, 합성규산알루미늄, 스테아린산, 스테아린산마그네슘, 스테아린산 알루미늄, 스테아린산칼슘, 백당, 덱스트로스, 소르비톨 및 탈크 등이 사용될 수 있다.The pharmaceutical composition of the present invention may further include pharmaceutically acceptable additives, wherein the pharmaceutically acceptable additives include starch, gelatinized starch, microcrystalline cellulose, lactose, povidone, colloidal silicon dioxide, and hydrogen phosphate. Calcium, Lactose, Mannitol, Taffy, Gum Arabic, Pregelatinized Starch, Corn Starch, Powdered Cellulose, Hydroxypropyl Cellulose, Opadry, Sodium Starch Glycolate, Carnauba Wax, Synthetic Aluminum Silicate, Stearic Acid, Magnesium Stearate, Aluminum Stearate, Calcium stearate, white sugar, dextrose, sorbitol and talc may be used.
또한, 본 발명에 따른 약학 조성물의 투여량은 투여 경로, 질병의 정도, 성별, 체중, 나이 등에 따라서 증감될 수 있다. 따라서, 상기 투여량은 어떠한 면으로든 본 발명의 범위를 한정하는 것은 아니다.In addition, the dosage of the pharmaceutical composition according to the present invention may be increased or decreased depending on the route of administration, the degree of disease, gender, weight, age, and the like. Accordingly, the dosage is not intended to limit the scope of the present invention in any way.
상기 본 발명의 약학 조성물은 약제학적으로 유효한 양으로 투여될 수 있는데, 본 발명의 용어 "약제학적으로 유효한 양"이란 의학적 치료 또는 예방에 적용 가능한 합리적인 수혜/위험 비율로 질환을 치료 또는 예방하기에 충분한 양을 의미하며, 유효 용량 수준은 질환의 중증도, 약물의 활성, 환자의 연령, 체중, 건강, 성별, 환자의 약물에 대한 민감도, 사용된 본 발명 조성물의 투여 시간, 투여 경로 및 배출 비율 치료기간, 사용된 본 발명의 조성물과 배합 또는 동시 사용되는 약물을 포함한 요소 및 기타 의학 분야에 잘 알려진 요소에 따라 결정될 수 있다. 본 발명의 약학 조성물은 단독으로 투여하거나 공지된 면역치료제와 병용하여 투여될 수 있다. 상기 요소를 모두 고려하여 부작용 없이 최소한의 양으로 최대 효과를 얻을 수 있는 양을 투여하는 것이 중요하다.The pharmaceutical composition of the present invention can be administered in a pharmaceutically effective amount, the term "pharmaceutically effective amount" of the present invention is a reasonable benefit / risk ratio applicable to medical treatment or prevention to treat or prevent disease It means a sufficient amount, and the effective dose level is the severity of the disease, the activity of the drug, the patient's age, weight, health, sex, the patient's sensitivity to the drug, the administration time of the composition of the present invention used, the route of administration and the excretion rate Treatment It may be determined according to factors including duration, drugs used in combination or simultaneous use with the composition of the present invention used, and other factors well known in the medical arts. The pharmaceutical composition of the present invention may be administered alone or in combination with known immunotherapeutic agents. It is important to administer the amount that can obtain the maximum effect with the minimum amount without side effects in consideration of all the above factors.
본 발명의 다른 일 실시예에 따른 식품 조성물은 상기 조성물을 포함하여 제조된 것이다.A food composition according to another embodiment of the present invention is prepared by including the composition.
본 발명에 따른 식품 조성물은 분말, 과립, 정제, 캡슐, 시럽 또는 음료의 형태로 제공될 수 있으며, 이외에 다른 식품 또는 식품 첨가물과 함께 사용되고, 통상적인 방법에 따라 적절하게 사용될 수 있다. 유효성분의 혼합 양은 그의 사용 목적 예를 들어 예방, 건강 또는 치료적 처치에 따라 적합하게 결정될 수 있다.The food composition according to the present invention may be provided in the form of powder, granule, tablet, capsule, syrup or beverage, and may be used together with other foods or food additives, and may be appropriately used according to conventional methods. The mixing amount of the active ingredient may be appropriately determined depending on the purpose of use thereof, for example, prevention, health, or therapeutic treatment.
구체적인 예로, 상기 식품 조성물을 이용하여 근육질환의 예방 또는 개선 활성을 증강시킬 수 있는 가공식품을 제조할 수 있다. 이런 가공식품에는 예를 들어, 과자, 음료, 주류, 발효식품, 통조림, 우유가공식품, 육류가공식품, 국수 등을 포함한다. 과자는 비스킷, 파이, 케익, 빵, 캔디, 젤리, 껌, 시리얼 등을 포함한다. 음료는 음용수, 탄산음료, 기능성 이온음료, 기능성 이온음료, 주스(예를 들어, 사과, 배, 포도, 알로에, 감귤, 복숭아, 당근, 토마토 주스 등), 식혜 등을 포함한다. 주류는 청주, 위스키, 소주, 맥주, 양주, 과실주 등을 포함한다. 발효식품은 간장, 된장, 고추장 등을 포함한다. 통조림은 수산물 통조림(예들 들어, 참치, 고등어, 꽁치, 소라 통조림 등), 축산물 통조림(쇠고기, 돼지고기, 닭고기, 칠면조 통조림 등), 농산물 통조림(옥수수, 복숭아, 파일애플 통조림 등)을 포함한다. 우유가공식품은 치즈, 버터, 요구르트 등을 포함한다. 육류가공식품은 돈까스, 비프까스, 치킨까스, 소세지. 탕수육, 너겟류, 너비아니 등을 포함한다. 밀봉포장 생면 등의 국수를 포함한다. 이 외에도 상기 조성물은 레토르트식품, 스프류 등에 사용될 수 있다.As a specific example, a processed food capable of enhancing the activity of preventing or improving muscle diseases can be prepared using the food composition. Such processed foods include, for example, sweets, beverages, alcoholic beverages, fermented foods, canned foods, processed milk products, processed meat products, noodles, and the like. Sweets include biscuits, pies, cakes, breads, candies, jellies, chewing gum, cereals, and the like. Beverages include drinking water, carbonated beverages, functional ionic beverages, functional ionic beverages, juice (eg, apple, pear, grape, aloe, tangerine, peach, carrot, tomato juice, etc.), sikhye, and the like. Liquor includes rice wine, whiskey, soju, beer, liquor, fruit wine, and the like. Fermented foods include soy sauce, soybean paste, red pepper paste, and the like. Canned food includes canned seafood (e.g., canned tuna, mackerel, saury, canned conch, etc.), canned livestock products (canned beef, pork, chicken, turkey, etc.), canned agricultural products (canned corn, peaches, canned pineapple, etc.). Milk processed foods include cheese, butter, yogurt, and the like. Processed meat products include pork cutlet, beef cutlet, chicken cutlet, and sausage. Includes sweet and sour pork, nuggets, nubani, etc. It includes noodles such as sealed package raw noodles. In addition to this, the composition can be used for retort food, soup, and the like.
본 발명의 식품 조성물을 식품 첨가물로 사용하는 경우, 상기 식품 조성물을 그대로 첨가하거나 다른 식품 또는 식품성분과 함께 사용될 수 있고, 통상적인 방법에 따라 적절하게 사용될 수 있다. 유효성분은 그의 사용 목적(예방 또는 개선)에 따라 적절하게 사용될 수 있다.When the food composition of the present invention is used as a food additive, the food composition may be added as it is or used together with other foods or food ingredients, and may be appropriately used according to conventional methods. Active ingredients can be appropriately used depending on their purpose of use (prevention or improvement).
이 때, 식품 또는 음료 중의 상기 추출물의 양은 전체 식품 중량의 약 0.01 내지 15 중량%로 가할 수 있으며, 건강음료 조성물은 100 ㎖를 기준으로 약 0.01 내지 10g의 비율로 가할 수 있으나, 이에 제한되지 않는다.At this time, the amount of the extract in the food or beverage may be added at about 0.01 to 15% by weight of the total food weight, and the health drink composition may be added at a rate of about 0.01 to 10g based on 100 ml, but is not limited thereto .
본 발명의 식품 조성물이 음료 조성물인 경우, 지시된 비율로 필수 성분으로서 상기 추출물을 함유하는 외에는 다른 성분에는 특별한 제한이 없으며 통상의 음료와 같이 여러 가지 향미제 또는 천연 탄수화물 등을 추가 성분으로서 함유할 수 있다. 상술한 천연 탄수화물의 예는 모노사카라이드, 예를 들어, 포도당, 과당 등; 디사카라이드, 예를 들어 말토스, 슈크로스 등; 및 폴리사카라이드, 예를 들어 덱스트린, 시클로덱스트린 등과 같은 통상적인 당, 및 자일리톨, 소르비톨, 에리트리톨 등의 당알코올이다. 상술한 것 이외의 향미제로서 천연 향미제 (타우마틴, 스테비아 추출물 (예를 들어 레바우디오시드 A, 글리시르히진등) 및 합성 향미제 (사카린, 아스파탐 등)를 사용할 수 있으나, 이에 제한되지 않을 수 있다. 상기 천연 탄수화물의 비율은 본 발명의 조성물 100 ㎖ 당 일반적으로 약 1 내지 20g의 범위일 수 있으나, 이에 제한되지 않을 수 있다.When the food composition of the present invention is a beverage composition, there is no particular limitation on other ingredients except for containing the extract as an essential ingredient in the indicated ratio, and it may contain various flavoring agents or natural carbohydrates as additional ingredients like conventional beverages. can Examples of the aforementioned natural carbohydrates include monosaccharides such as glucose, fructose, and the like; disaccharides such as maltose, sucrose and the like; and polysaccharides such as conventional sugars such as dextrins, cyclodextrins, and the like, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (thaumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) may be used, but are not limited thereto. The ratio of the natural carbohydrates may generally range from about 1 to about 20 g per 100 ml of the composition of the present invention, but may not be limited thereto.
상기 외에 본 발명의 조성물은 여러 가지 영양제, 비타민, 광물 (전해질), 합성 풍미제 및 천연 풍미제 등의 풍미제, 착색제 및 중진제 (치즈, 초콜릿 등), 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, Ph 조절제, 안정화제, 방부제, 글리세린, 알코올, 탄산 음료에 사용되는 탄산화제 등을 함유할 수 있으나, 이에 제한되지 않을 수 있다.In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and enhancers (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its It may contain salts, organic acids, protective colloidal thickeners, pH regulators, stabilizers, preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages, and the like, but may not be limited thereto.
그 밖에 본 발명의 조성물은 천연 과일 주스 및 과일 주스 음료 및 야채 음료의 제조를 위한 과육을 함유할 수 있고, 이러한 성분은 독립적으로 또는 조합하여 사용할 수 있다. 이러한 첨가제의 비율은 중요하지는 않지만 본 발명의 조성물 100 중량부 당 약 0.1 내지 약 20 중량부의 범위에서 선택될 수 있으나, 이에 제한되지 않는다.In addition, the composition of the present invention may contain natural fruit juice and fruit flesh for producing fruit juice beverages and vegetable beverages, and these components may be used independently or in combination. The ratio of these additives is not critical, but may be selected in the range of about 0.1 to about 20 parts by weight per 100 parts by weight of the composition of the present invention, but is not limited thereto.
본 발명에서 말하는 기능성 식품은 건강보조의 목적으로 특정성분을 원료로 하거나 식품원료에 들어있는 특정성분을 추출, 농축, 정제, 혼합 등의 방법으로 제조, 가공한 식품으로 건강보조식품을 포함하며, 기타, 식품성분이 갖는 생체방어, 생체리듬의 조절, 질병의 방지와 회복 등 생체조절기능을 생체에 대하여 충분히 발휘할 수 있도록 설계되고 가공된 식품으로서, 질병의 예방 및 질병의 회복 등과 관련된 기능도 갖는 것을 모두 포함하는 것으로 한다.The functional food referred to in the present invention is a food manufactured and processed by extracting, concentrating, refining, mixing, etc., a specific ingredient as a raw material or a specific ingredient contained in a food raw material for the purpose of health supplementation, and includes health supplement food. In addition, it is a food designed and processed to sufficiently exert the biological control functions of food components, such as bio-defense, regulation of biological rhythm, prevention and recovery of disease, etc., with functions related to disease prevention and recovery. make it all inclusive.
상기 건강기능식품의 종류에 특별한 제한은 없다. 본 발명의 식품 조성물은 식품, 특히 기능성 식품으로 제조될 수 있다. 본 발명의 기능성 식품은 식품 제조 시에 통상적으로 첨가되는 성분을 포함하며, 예를 들어, 단백질, 탄수화물, 지방, 영양소 및 조미제를 포함한다. 예컨대, 드링크제로 제조되는 경우에는 유효성분 이외에 천연 탄수화물 또는 향미제를 추가 성분으로서 포함시킬 수 있다. 상기 천연 탄수화물은 모노사카라이드(예컨대, 글루코오스, 프럭토오스 등), 디사카라이드(예컨대, 말토스, 수크로오스 등), 올리고당, 폴리사카라이드(예컨대, 덱스트린, 시클로덱스트린 등) 또는 당알코올(예컨대, 자일리톨, 소르비톨, 에리쓰리톨 등)인 것이 바람직하다. 상기 향미제는 천연 향미제(예컨대, 타우마틴, 스테비아 추출물 등)와 합성 향미제(예컨대, 사카린, 아스파르탐 등)를 이용할 수 있다. 상기 건강기능식품 조성물 이외에 여러 가지 영양제, 비타민, 전해질, 풍미제, 착색제, 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알콜, 탄산음료에 사용되는 탄산화제 등을 더 함유할 수 있다.There is no particular limitation on the type of health functional food. The food composition of the present invention can be made into food, particularly functional food. The functional food of the present invention includes components commonly added during food preparation, and includes, for example, proteins, carbohydrates, fats, nutrients, and seasonings. For example, when prepared as a drink, natural carbohydrates or flavors may be included as additional ingredients in addition to active ingredients. The natural carbohydrates are monosaccharides (eg, glucose, fructose, etc.), disaccharides (eg, maltose, sucrose, etc.), oligosaccharides, polysaccharides (eg, dextrins, cyclodextrins, etc.) or sugar alcohols (eg, maltose, sucrose, etc.) , xylitol, sorbitol, erythritol, etc.) are preferred. As the flavoring agent, natural flavoring agents (eg, thaumatin, stevia extract, etc.) and synthetic flavoring agents (eg, saccharin, aspartame, etc.) may be used. In addition to the health functional food composition, various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonic acid It may further contain a carbonation agent used in beverages and the like.
본 발명은 애기우산나물 추출물을 유효성분으로 포함하는 근육질환의 예방 또는 치료용 조성물에 관한 것이다. 보다 상세하게는 애기우산나물 추출물을 유효성분으로 포함하여 근 감소와 관련한 파골세포의 분화를 저하하고 근 섬유 형성을 촉진함으로써 근육량을 증가 효과를 갖는 동시에 복용량 및 복용기간이 증가하여도 부작용의 문제가 없는 근육질환의 예방 또는 치료용 조성물에 관한 것이다.The present invention relates to a composition for preventing or treating muscle diseases comprising an extract of Arabidopsis japonica as an active ingredient. More specifically, it contains Arabidopsis extract as an active ingredient to reduce the differentiation of osteoclasts related to muscle loss and promote muscle fiber formation, thereby increasing muscle mass and eliminating side effects even when the dose and duration of administration are increased. It relates to a composition for preventing or treating muscle diseases without.
이하, 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자가 용이하게 실시할 수 있도록 본 발명의 실시예에 대하여 상세히 설명한다. 그러나 본 발명은 여러 가지 상이한 형태로 구현될 수 있으며 여기에서 설명하는 실시예에 한정되지 않는다.Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement the present invention. However, the present invention may be embodied in many different forms and is not limited to the embodiments described herein.
[제조예 1: 천연 추출물의 제조][Preparation Example 1: Preparation of natural extract]
1. 애기우산나물 추출물의 제조1. Preparation of Arabidopsis extract
애기우산나물을 세척하고 건조한 뒤 이를 분쇄하였다. 상기 분쇄물을 에탄올에 혼합하고 이를 2시간 동안 98 내지 100℃를 유지하여, 냉각시킨 뒤 와트만 여과지로 여과하여 그 여액을 수득하였다. 이를 애기우산나물 추출물(ESA)로 제조하였다. After washing and drying the Arabidopsis, it was pulverized. The pulverized product was mixed with ethanol, maintained at 98 to 100° C. for 2 hours, cooled, and then filtered with a Whatman filter paper to obtain the filtrate. This was prepared with Arabidopsis extract (ESA).
2. 기타 천연 추출물의 제조2. Preparation of other natural extracts
상기 애기우산나물 추출물(ESA)의 제조방법과 동일한 방법을 이용하여, 덜꿩나무 추출물(EVE), 파드득나물 추출물(ECJ) 및 털중나리 추출물(ELA)를 제조하였다.Using the same method as the manufacturing method of the Arabidopsis chinensis extract (ESA), an extract of the pheasant tree (EVE), an extract of Padduk herb (ECJ), and an extract (ELA) were prepared.
3. 혼합 추출물의 제조 3. Preparation of Mixed Extracts
상기 제조된 애기우산나물 추출물(ESA), 덜꿩나무 추출물(EVE), 파드득나물 추출물(ECJ) 및 털중나리 추출물(ELA)를 하기 표 1과 같은 중량 범위 내로 혼합하여 혼합 추출물을 제조하였다.A mixed extract was prepared by mixing the above-prepared Arabidopsis extract (ESA), Ephesus japonica extract (EVE), Padduk herb extract (ECJ), and Elysium japonica extract (ELA) within the weight range shown in Table 1 below.
(단위: 중량부)(unit: parts by weight)
[실시예 1: 근 섬유 형성 촉진 효과 실험][Example 1: muscle fiber formation promoting effect test]
근모세포(Myoblast)의 준비Preparation of myoblasts
5 주령 수컷 CrljOri : CD1 (ICR) 마우스를 희생시킨 다음 뒷다리 근육 조직을 수득하였다. 근육 조직을 메스로 5 분 동안 다졌다. 다진 근육 용액을 37 ℃에서 60 분 동안 배양한 후 1500 rpm에서 5 분 동안 원심 분리하였다. 수득 된 세포를 염기성 FGF (2.5ng/ml) 존재하에 20 % 열불활성화된 말 혈청과 50unit/ml의 페니실린을 함유하는 Ham 's F-10 배지로 젤라틴 코팅없이 1 시간 동안 세포 배양 접시에 도말하였다. 1 시간 후, 부유 세포를 젤라틴 코팅없이 2 차 세포 배양 접시로 옮기고 1 시간 동안 배양하였다. 이 과정을 반복한 다음 세포를 젤라틴 코팅 접시로 옮겼다. 배양된 세포는 근모세포로 사용되었다.Five-week-old male CrljOri:CD1 (ICR) mice were sacrificed and hind limb muscle tissues were obtained. The muscle tissue was minced with a scalpel for 5 minutes. The minced muscle solution was incubated at 37 °C for 60 minutes and then centrifuged at 1500 rpm for 5 minutes. The obtained cells were plated on cell culture dishes for 1 hour without gelatin coating with Ham's F-10 medium containing 20% heat-inactivated horse serum and 50 units/ml penicillin in the presence of basic FGF (2.5ng/ml). . After 1 hour, the floating cells were transferred to a secondary cell culture dish without gelatin coating and incubated for 1 hour. After repeating this process, cells were transferred to gelatin-coated dishes. Cultured cells were used as myoblasts.
근모세포(Myoblast)의 분화(근 섬유 형성)Differentiation of myoblasts (muscle fiber formation)
근육 조직에서 얻은 근모세포를 10% 마트리겔(Matrigel)이 전처리된 48 웰(well) 조직배양 플레이트에 well 당 3 x 104개씩 배양하였다. 약 24시간 배양 후 성장 배양액을 5% 열불활성화된 말 혈청 및 50 unit/ml의 페니실린/스트렙토마이신을 포함하는 DEM 융합배지에서 추가로 배양하였다. 배지는 2일에 한번 교체되었다. 배양 후 48 웰의 세포를 PBS(phosphate buffered saline)로 세척하고 70% 에탄올을 이용하여 고정시켰다. 에탄올이 제거되고 톨루이딘 블루(Toluidine blue)와 푹신(Fuchsin)을 포함하는 LADD 염색 시약을 이용하여 형성된 근 섬유를 염색하였다. 1분간 인큐베이션(incubation)한 후 염색된 세포들은 정제수로 LADD 염색용액이 물에 침출되지 않을 때까지 증류수로 세척하였다. 세척후 세포를 실온에서 10분간 건조시켰다.Myoblasts obtained from muscle tissue were cultured 3 x 10 4 per well in a 48-well tissue culture plate pre-treated with 10% Matrigel. After culturing for about 24 hours, the growth medium was further cultured in a DEM fusion medium containing 5% heat-inactivated horse serum and 50 units/ml of penicillin/streptomycin. Medium was changed once every 2 days. After culturing, cells in 48 wells were washed with PBS (phosphate buffered saline) and fixed using 70% ethanol. Ethanol was removed and the formed muscle fibers were stained using LADD staining reagent including toluidine blue and Fuchsin. After incubation for 1 minute, the stained cells were washed with distilled water until the LADD staining solution was not leached into the water. After washing, the cells were dried at room temperature for 10 minutes.
상기 LADD 염색으로 염색된 근관 (myotube)은 상기 제조예 1에서 제조한 애기우산나물 추출물(ESA, M1) 및 혼합 추출물 M2 내지 M5를 1mM의 농도로 처리한 후, 비히클(vehicle) 처리를 대조군으로 형성된 근관(myotube)의 핵 수를 측정하였다.The myotubes stained with the LADD stain were treated with the Arabidopsis extract (ESA, M1) and the mixed extracts M2 to M5 prepared in Preparation Example 1 at a concentration of 1 mM, followed by vehicle treatment as a control. The number of nuclei in the formed myotubes was measured.
그 결과를 하기 표 2에 나타내었다.The results are shown in Table 2 below.
(비히클(vehicle) 처리)control group
(vehicle processing)
(단위: 지수)(Unit: index)
상기 표 2에 나타난 바와 같이, 본 발명의 애기우산나물 추출물(ESA, M1) 및 혼합 추출물 M2 내지 M5은 비히클(vehicle)이 처리된 근관(myotube)의 핵 수에 비하여 근관(myotube)의 핵 수를 증가시켜, 근모세포의 융합을 촉진하였음을 알 수 있었으며, 이를 통해 본 발명의 조성물은 금 섬유 형성 촉진 효과를 나타내는 것을 확인하였다.As shown in Table 2, the number of myotube nuclei in the Arabidopsis extracts (ESA, M1) and the mixed extracts M2 to M5 of the present invention is compared to the number of nuclei in the vehicle-treated myotube. By increasing the , it was found that the fusion of myoblasts was promoted, and through this, it was confirmed that the composition of the present invention exhibits an effect of promoting gold fiber formation.
특히, 애기우산나물 추출물(ESA, M1)만을 포함하는 경우에 비해, M2 내지 M5에 의하는 경우, 효과가 보다 향상되는 것을 확인하였으며, M4에서 그 효과가 극대화됨을 확인하였다.In particular, compared to the case of containing only Arabidopsis extracts (ESA, M1), it was confirmed that the effect was further improved in the case of M2 to M5, and the effect was maximized in M4.
[실시예 2: 파골세포 분화 억제 효과 실험][Example 2: Osteoclast differentiation inhibitory effect test]
골수유래 대식세포 (Bone marrow-derived macrophage, BMM) 준비Preparation of bone marrow-derived macrophage (BMM)
5 주령 수컷 CrljOri : CD1 (ICR) 마우스를 희생시킨 다음 마우스에서 대퇴골과 경골을 채취하였다. 골수세포는 대퇴골과 경골에서 골수를 씻어내어 얻었다. 비부착 성 골수 세포를 α-MEM과 함께 3 일 동안 M-CSF (30 ng/ml)로 추가 배양하여 골수유래 대식세포(BMM)를 생성하였다.Five-week-old male CrljOri:CD1 (ICR) mice were sacrificed, and femurs and tibias were harvested from the mice. Bone marrow cells were obtained by washing the bone marrow from the femur and tibia. Non-adherent bone marrow cells were further cultured with α-MEM and M-CSF (30 ng/ml) for 3 days to generate bone marrow-derived macrophages (BMMs).
파골세포 분화 및 Tartrate-resistant acid phosphatase(TRAP) 염색Osteoclast differentiation and tartrate-resistant acid phosphatase (TRAP) staining
파골세포를 생성하기 위해, 상기 골수유래 대식세포 (4 x 104 세포/웰)을 M-CSF (30 ng/ml) 및 RANKL (100ng/ml)의 존재 하에 10 %(v/v) 열불활성화 된 소태아혈청(FBS) 및 50 unit/ml의 페니실린/스트렙토마이신을 함유하는 α-MEM 컴플리트배지(complete medium)로 4 일 동안 48 웰 플레이트에서 배양하였다.To generate osteoclasts, the bone marrow-derived macrophages (4 x 10 4 cells/well) were inactivated by 10% (v/v) heat in the presence of M-CSF (30 ng/ml) and RANKL (100 ng/ml). The cells were cultured in a 48-well plate for 4 days with α-MEM complete medium containing FBS and 50 units/ml of penicillin/streptomycin.
배지는 매 3 일마다 교환하였다. 다핵세포(MNC)는 4 일째에 관찰되었다. 세포를 10 % 포르말린 용액에 20 분 동안 고정하고 0.1 % Triton X-100으로 1분 동안 투과화(permeabilized)시켰다. PBS로 2 회 세척한 후 시그마알드리치사(Sigma-Aldrich)의 Leukocyte Acid Phosphatase Assay Kit를 사용하여 제조업체의 지침에 따라 세포를 염색하였다. 세포 내 핵의 개수가 5개 이상인 파골세포를 기준으로 well당 분화된 파골세포의 숫자를 파악하여 분화 정도를 평가하였다.Medium was changed every 3 days. Multinucleated cells (MNC) were observed on day 4. Cells were fixed in 10% formalin solution for 20 minutes and permeabilized with 0.1% Triton X-100 for 1 minute. After washing twice with PBS, the cells were stained using Sigma-Aldrich's Leukocyte Acid Phosphatase Assay Kit according to the manufacturer's instructions. The degree of differentiation was evaluated by identifying the number of differentiated osteoclasts per well based on osteoclasts having 5 or more nuclei in the cell.
그 결과를 하기 표 3에 나타냈었다.The results were shown in Table 3 below.
(무처리)control group
(untreated)
(단위: 지수)(Unit: Index)
상기 표 3에 나타난 바와 같이, 본 발명의 애기우산나물 추출물(ESA, M1) 및 혼합 추출물 M2 내지 M5은 대조군인 무처리군에 비해, 골수유래 대식세포의 파골 세포로의 분화가 억제되었음을 확인하였다.As shown in Table 3, it was confirmed that the Arabidopsis extracts (ESA, M1) and the mixed extracts M2 to M5 of the present invention inhibited the differentiation of bone marrow-derived macrophages into osteoclasts, compared to the control group, which was untreated. .
특히, 애기우산나물 추출물(ESA, M1)만을 포함하는 경우에 비해, M2 내지 M5에 의하는 경우, 효과가 보다 향상되는 것을 확인하였으며, M4에서 그 효과가 극대화됨을 확인하였다.In particular, compared to the case of containing only Arabidopsis extracts (ESA, M1), it was confirmed that the effect was further improved in the case of M2 to M5, and the effect was maximized in M4.
[실시예 3: 기호도 평가][Example 3: Evaluation of preference]
상기 실시예 1 내지 2에서 확인한 본 발명의 애기우산나물 추출물(ESA, M1) 및 혼합 추출물 M2 내지 M5에 대한 관능성 평가를 위해, 이를 차(tea)로 제조하여 20 내지 70대 성인남녀 30명을 대상으로 맛, 향 및 종합기호도 평가를 요청하였다.For the sensory evaluation of the Arabidopsis extract (ESA, M1) and the mixed extract M2 to M5 of the present invention confirmed in Examples 1 to 2, they were prepared into tea and 30 adult men and women in their 20s to 70s were requested to evaluate taste, aroma and overall preference.
상기 관능성 평가는 1 내지 10의 지수로 평가를 요청하고, 그 점수가 높을수록 기호도가 우수한 것이다. 한편, 상대적 비교를 위해 T1의 경우를 지수 3으로 설정하였다. The sensory evaluation is requested by an index of 1 to 10, and the higher the score, the better the preference. Meanwhile, for relative comparison, the case of T1 was set to an index of 3.
그 결과는 하기 표 4과 같다.The results are shown in Table 4 below.
(단위: 지수)(Unit: index)
상기 표 4에 나타난 바와 같이, 애기우산나물 추출물(ESA, M1만을 포함하는 경우에 비해 혼합 조성물 M2 내지 M5의 경우 맛과 향에 대한 평가가 우수함을 확인할 수 있다. As shown in Table 4, it can be seen that the evaluation of taste and aroma is excellent in the mixed composition M2 to M5 compared to the case containing only the Arabidopsis extract (ESA and M1).
특히 M3 내지 M4에 의하는 경우, 기호도가 우수한 것으로 평가되었으며, M4의 기호도 평가가 가장 우수한 것을 알 수 있다.In particular, in the case of M3 to M4, preference was evaluated as excellent, and it can be seen that the preference evaluation of M4 was the best.
즉, 상기 범위의 혼합 조성물의 경우, 근육질환의 예방 또는 치료 효과뿐만 아니라, 이를 건강기능식품 등에 이용하는 경우 기호도 또한 극대화됨을 확인할 수 있다.That is, in the case of the mixed composition in the above range, it can be confirmed that not only the preventive or therapeutic effect of muscle diseases, but also the preference when used for health functional foods, etc.
이상에서 본 발명의 바람직한 실시예에 대하여 상세하게 설명하였지만 본 발명의 권리범위는 이에 한정되는 것은 아니고 다음의 청구범위에서 정의하고 있는 본 발명의 기본 개념을 이용한 당업자의 여러 변형 및 개량 형태 또한 본 발명의 권리범위에 속하는 것이다.Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements of those skilled in the art using the basic concept of the present invention defined in the following claims are also made according to the present invention. falls within the scope of the rights of
Claims (7)
근육질환의 예방 또는 치료용 조성물.Contains the extract of Syneilesis aconitifolia (Bunge) Maxim as an active ingredient
A composition for preventing or treating muscle diseases.
상기 추출물은 물, C1 내지 C6의 저급 알코올 및 이들의 혼합물로 이루어진 군으로부터 선택된 추출 용매를 이용하여 추출되는
근육질환의 예방 또는 치료용 조성물.According to claim 1,
The extract is extracted using an extraction solvent selected from the group consisting of water, C 1 to C 6 lower alcohols, and mixtures thereof.
A composition for preventing or treating muscle diseases.
상기 근육질환은 긴장감퇴증(atony), 근위축증(muscular atrophy), 근이영양증(muscular dystrophy), 근무력증, 악액질(cachexia), 경직성 척추 증후군(rigid spinesyndrome), 근위축성 측삭경화증(amyotrophic lateral sclerosis), 샤르코-마리-투스병(Charcot-Marie-Tooth disease) 및 근육 감소증(sarcopenia)으로 이루어진 군으로부터 선택되는 어느 하나 이상인 것을 특징으로 하는
근육질환의 예방 또는 치료용 조성물.According to claim 1,
The muscle disease is atony, muscular atrophy, muscular dystrophy, myasthenia, cachexia, rigid spine syndrome, amyotrophic lateral sclerosis, Charcot- Characterized in that at least one selected from the group consisting of Charcot-Marie-Tooth disease and sarcopenia
A composition for preventing or treating muscle diseases.
상기 조성물은 파골세포의 분화 억제능을 나타내는
근육질환의 예방 또는 치료용 조성물.According to claim 1,
The composition shows the ability to inhibit the differentiation of osteoclasts
A composition for preventing or treating muscle diseases.
상기 조성물은 근 섬유 형성 촉진 효과를 통해 근육량(muscle mass)을 증가시키는
근육질환의 예방 또는 치료용 조성물.According to claim 1,
The composition increases muscle mass through the effect of promoting muscle fiber formation.
A composition for preventing or treating muscle diseases.
약학 조성물.Claims 1 to 5, comprising a composition for the treatment of muscle disease according to any one of the preceding
pharmaceutical composition.
식품 조성물.
Claims 1 to 5, comprising a composition for the prevention of muscle disease according to any one of the preceding
food composition.
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WO2007004390A1 (en) * | 2005-07-06 | 2007-01-11 | Kenji Kohno | Cachexia improving preparation and cachexia improving food |
CN104887966A (en) * | 2015-06-07 | 2015-09-09 | 王彬训 | Traditional Chinese medicine preparation for treating lumbar muscle strain and preparation method thereof |
KR20150110135A (en) * | 2014-03-24 | 2015-10-02 | 한국식품연구원 | Pharmaceutical or food composition comprising plant extracts with secretory activity of neuropeptides |
KR20170013543A (en) * | 2015-07-28 | 2017-02-07 | 건국대학교 글로컬산학협력단 | A pharmaceutical composition comprising extract of Syneilesis palmata for prevention and treatment of inflammatory diseases |
KR20170132705A (en) | 2015-05-26 | 2017-12-04 | (주)앗코스텍 | Composition for prevention, improvement or treatment of muscular disorder or improvement of muscular functions |
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WO2007004390A1 (en) * | 2005-07-06 | 2007-01-11 | Kenji Kohno | Cachexia improving preparation and cachexia improving food |
KR20150110135A (en) * | 2014-03-24 | 2015-10-02 | 한국식품연구원 | Pharmaceutical or food composition comprising plant extracts with secretory activity of neuropeptides |
KR20170132705A (en) | 2015-05-26 | 2017-12-04 | (주)앗코스텍 | Composition for prevention, improvement or treatment of muscular disorder or improvement of muscular functions |
CN104887966A (en) * | 2015-06-07 | 2015-09-09 | 王彬训 | Traditional Chinese medicine preparation for treating lumbar muscle strain and preparation method thereof |
KR20170013543A (en) * | 2015-07-28 | 2017-02-07 | 건국대학교 글로컬산학협력단 | A pharmaceutical composition comprising extract of Syneilesis palmata for prevention and treatment of inflammatory diseases |
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