KR20230022194A - Portable constant temperature apparatus for RNA amplification - Google Patents

Abstract

The present invention relates to a portable thermostat for RNA amplification, including: a housing having a receiving space formed therein, provided with an opening opened upwardly and an air permeation hole, and including a cover for opening/closing the opening; a thermal block installed in the receiving space, formed to have at least one mounting groove to open upwards for mounting an Eppendorf tube at the side exposed through the opening and made of a heat conductive material; a heat-emitting unit configured to provide heat to the thermal block and to supply heat to the Eppendorf tube; a heat radiating fan configured to discharge the heat of the thermal block in the receiving space through the air permeation hole by air blowing power; a temperature sensor configured to measure the temperature of the thermal block and to output a detection signal; a controlling unit configured to receive the detection signal from the temperature sensor and to control the operation of the heat-emitting unit and the heat radiating fan so that the thermal block may retain a predetermined temperature or may perform predetermined thermal cycling; and a power supply unit for supplying electric power required for the operation. According to the present invention, the portable thermostat for RNA amplification has a significantly simple structure and significantly reduced size, maintains a temperature of about 60 ℃ with the Eppendorf tube inserted thereto to allow RNA amplification and thus can be used for a PCR thermocycling process, shows excellent temperature-retaining performance, is easily portable, and can be purchased at a low cost to allow cost-efficient use.

Description

Portable constant temperature apparatus for RNA amplification

The present invention relates to a portable thermostat for RNA amplification, and more particularly, has a very simple structure and greatly reduced size, can be used for PCR thermocycling techniques, has excellent temperature maintenance performance, and is portable. It relates to a portable thermostat for RNA amplification that is convenient and inexpensive to purchase, so that it can be used as a self-diagnosis kit.

In general, various methods for diagnosing corona virus infection in a short time have been proposed, and in particular, reagents for determining infection using RNA instead of DNA are being developed. In order to detect corona virus using DNA, PCR thermocycling technique must be used, and for this, a thermal block thermocycler equipped with a sophisticated multi-stage temperature control function is required.

As a technology related to a conventional thermal block thermocycler, Korean Patent Registration No. 10-0790004 "PCR device using constant-temperature metal block and its method" has been disclosed. This is a PCR device using a constant-temperature metal block, comprising: a PCR tube containing materials for synthesizing DNA; a rotor having a plurality of rotor specimen grooves in which the PCR tubes are mounted; a plurality of metal blocks for maintaining a constant temperature maintained at temperatures required for each stage of DNA synthesis and having a rotor rotation groove formed on one side; one or more metal blocks for cooling, which are located between the metal blocks for maintaining constant temperature, have a rotor rotation groove formed on one side, and lower the temperature; one or more metal blocks for heating, which are located between the metal blocks for maintaining constant temperature, have a rotor rotation groove formed on one side, and increase the temperature; And the plurality of metal blocks for maintaining constant temperature, the one or more metal blocks for cooling, and the one or more metal blocks for heating are arranged in an annular shape, but arranged in order according to the section requiring temperature increase and decrease in the DNA synthesis step, It is configured to include; a body configured so that the rotor equipped with the PCR tube rotates along the rotor rotation groove with a metal block having a temperature suitable for the reaction step.

However, the incubator for use in the conventional PCR thermocycling technique as well as the prior art has the disadvantage of being too expensive to construct a self-diagnosis kit, and is difficult to carry, resulting in limitations in its use. I had.

In order to solve the problems of the prior art described above, the present invention has a very simple structure, greatly reduces the size, inserts an eppendolph tube and maintains a temperature around 60 ° C., so that RNA amplification is achieved And, as a result, it can be used for PCR thermocycling technique, has excellent temperature maintenance performance, is easy to carry, and has low purchase cost, so it can be used economically, so that it can be used as a cheap self-diagnosis kit has a purpose

In order to solve the above problems, according to one aspect of the present invention, a housing having an accommodation space formed therein, an opening opening upward, a ventilation hole provided, and a cover provided to open and close the opening. ; a thermal block installed in the accommodating space, formed so that at least one mounting groove for mounting an Eppendorf tube is opened upward on a side exposed through the opening, and made of a thermally conductive material; a heating unit provided to supply heat to the Eppendorf tube by providing heat to the thermal block; a heat dissipation fan installed in the accommodation space to discharge the heat of the thermal block through the ventilation hole by a blowing force; a temperature sensor installed to measure the temperature of the thermal block and output a detection signal; a control unit that receives a detection signal from the temperature sensor and controls operations of the heating unit and the heat dissipation fan so that the thermal block maintains a predetermined temperature or performs predetermined thermal cycling; and a power supply unit for supplying power required for operation.

The thermal block may be formed such that a plurality of mounting grooves are vertically opened upwards, the heat generating part may be attached to one side, and the temperature sensor may be attached to the other side.

In the thermal block, fixing ribs are provided at the bottom so as to protrude horizontally to both sides, and each of the fixing ribs is fixed to a fixing boss extending downward from both sides of the opening in the accommodation space with fixing bolts, thereby forming the opening. It is fixed so as to be exposed, and may be installed to be spaced apart from the bottom surface of the accommodation space.

A connector provided to be connected to a cable from an external terminal; and a communication module provided to be connected to an external terminal through wireless communication, wherein the control unit controls to perform an operation corresponding to an operation signal transmitted from the terminal through the connector or the communication module. can

a switching unit provided to input an operation signal to an outer surface of the housing; a display unit provided on an outer surface of the housing to display an operating state to the outside; and a display unit provided on an outer surface of the housing to externally display the connection by the communication module, wherein the control unit controls to perform an operation corresponding to an operation signal input by the switching unit, The display unit may be controlled to display an operating state, and the display unit may be controlled according to whether or not the communication module is connected.

The controller, in order to control the temperature of the thermal block, inputs the output voltage of the temperature sensor corresponding to the target temperature and the reference voltage of 0.6V to the inverting input terminal and the non-inverting input terminal of the operational amplifier, respectively, and differentially compares the reference voltage, When the voltage is higher than the voltage of the temperature sensor, the switch supplying current to the heating part is turned on, and when the reference voltage is lower than the voltage of the temperature sensor, the switch supplying current to the heating part is turned off. control can be performed.

a light emitting unit installed at one side of the mounting groove to emit light toward the Eppendorf tube; and a light receiving unit configured to receive light emitted from the light emitting unit and pass through the Eppendorf tube and provide a corresponding detection signal, wherein the control unit receives the detection signal from the light receiving unit and controls the Eppendorf Absorbance change during the amplification and color development reaction period in the tube may be measured and provided to a display unit or an external terminal.

A fluorescence receiver installed on one side of the mounting groove and measuring fluorescence light emitted from the fluorochrome reaction in the Eppendorf tube and providing a corresponding detection signal, wherein the control unit includes a detection signal of the fluorescence receiver may be received, and a fluorescent dye reaction in the Eppendorf tube may be measured and provided to a display unit or an external terminal.

It is provided on the PCB in the housing and further includes a selection unit for switching to select constant temperature amplification and thermal cycling tailored to various diagnostic reagents, wherein the control unit receives a signal of switching selected by the selection unit and performs the switching. Operations of the heating unit and the heat dissipation fan may be controlled to perform constant temperature or thermal cycling corresponding to a signal of .

According to the portable thermostat for RNA amplification according to the present invention, while having a very simple structure and drastically reducing the size, by inserting an Eppendorf tube and maintaining the temperature around 60 ° C., RNA amplification is achieved As a result, it can be used for PCR thermocycling techniques, has excellent temperature maintenance performance, is easy to carry, and is inexpensive to purchase, enabling economical use, which can be used as a cheap self-diagnosis kit has the effect of enabling

1 is a perspective view showing a portable thermostat for RNA amplification according to an embodiment of the present invention.
FIG. 2 is a perspective view showing an open cover of a portable thermostat for RNA amplification according to an embodiment of the present invention.
3 is a side cross-sectional view showing a portable thermostat for RNA amplification according to an embodiment of the present invention.
4 is a configuration diagram showing a portable thermostat for RNA amplification according to an embodiment of the present invention.
5 is a configuration diagram showing temperature control by a control unit of a portable thermostat for RNA amplification according to an embodiment of the present invention.
6 is a perspective view showing main parts of a portable thermostat for RNA amplification according to an embodiment of the present invention.
7 is an image showing temperature control characteristics of a portable thermostat for RNA amplification according to an embodiment of the present invention.
8 is a side cross-sectional view showing a main part of a thermal block of a portable thermostat for RNA amplification according to an embodiment of the present invention.

Since the present invention may have various embodiments by various changes, it will be described by showing specific embodiments in the drawings as examples. In addition, it should be understood that the present invention is not limited to these specific examples, and includes all changes, equivalents, or substitutes included in the technical spirit of the present invention.

Hereinafter, the embodiments according to the present invention will be described in detail with reference to the accompanying drawings, and the same reference numerals are assigned to the same or corresponding components regardless of reference numerals, and redundant descriptions thereof are omitted. do it with

1 is a perspective view showing a portable thermostat for RNA amplification according to an embodiment of the present invention, and FIG. 2 shows a lid opening of the portable thermostat for RNA amplification according to an embodiment of the present invention. 3 is a side cross-sectional view showing a portable thermostat for RNA amplification according to an embodiment of the present invention, and FIG. 4 is a portable thermostat for RNA amplification according to an embodiment of the present invention. It is the configuration diagram shown.

1 to 4, the portable thermostat 100 for RNA amplification according to an embodiment of the present invention includes a housing 110, a thermal block 120, a heating unit 130, and a heat dissipating fan 117. ), a temperature sensor 140, a control unit 150 and a power supply unit.

The housing 110 has a receiving space 111 formed therein, has an opening 112 that opens upward, a ventilation hole 116 is provided, and a cover 115 is provided to open and close the opening 112. . In addition, the housing 110 is formed so that the opening 112 is opened at the upper end of the accommodation space 111, and the opening and closing part 114 is coupled to be detachable by fitting, hanging, or bolting to open and close the accommodation space 111. It can be made of various materials, including plastic.

The cover 115 serves to block foreign substances from entering the thermal block 120 during storage or carrying, and cools the head of the Eppendorf tube 10 having a large area during the temperature control process. In order to prevent a temperature gradient from occurring within (10), the upper portion of the housing 110 is blocked to insulate.

The thermal block 120 is installed in the accommodating space 111, and at least one mounting groove 121 for mounting the Eppendorf tube 10 on the side exposed through the opening 112 is formed to open upward, , may be made of a thermally conductive material. In this embodiment, the thermal block 120 is formed with two mounting grooves 121 so that two 1 to 2 ml Eppendorf tubes 10 are mounted, and is made of aluminum, which is a thermally conductive material. , and may be made of various metals or metal alloy materials, including copper having excellent thermal conductivity.

The thermal block 120 may be formed such that, for example, a plurality of mounting grooves 121 are vertically opened upwards, a heating unit 130 may be attached to one side, and a temperature sensor 140 may be attached to the other side. It can be attached (see Fig. 6). The heating unit 130 and the temperature sensor 140 are fixed to surface contact with both sides of the thermal block 120 by bolting, or in surface contact with both sides of the thermal block 120 through a thermal conductive adhesive or a separate bracket. Each can be fixed.

The thermal block 120 may be provided so that the fixing ribs 122 protrude horizontally to both sides at the lower end, and to the fixing bosses 113 extending downward from both sides of the opening 112 in the accommodation space 111, respectively. Each of the fixing ribs 122 may be fixed to be exposed to the opening 112 by being fixed with fixing bolts 123 . In this way, the thermal block 120 has a fixing structure in which the fixing ribs 122 are fixed to the fixing boss 113 and spaced apart from the bottom surface in the accommodation space 111, so that heat is transferred to the housing 110 side. This can contribute to preventing a user's burn due to overheating of the housing 110 while minimizing heat loss.

The heating unit 130 is installed to supply heat to the Eppendorf tube 10 mounted in the mounting groove 121 of the thermal block 120 by providing heat to the thermal block 120 . As the heating unit 130, for example, a shunt resistor for heating in the form of a TO-220 package may be used. This is an example, and various low-power heating elements may be used.

The heat dissipation fan 117 is installed to discharge the heat of the thermal block 120 in the accommodation space 111 through the ventilation hole 116 by a blowing force. The housing 110 may be provided with an inlet (not shown) for supplying outside air to the heat dissipation fan 117, and the vent 116 may be used for both intake and exhaust if necessary. In this way, the heat dissipation fan 117 crosses the thermal block 120 and blows wind through the ventilation hole 116 formed on the side wall of the housing 110, so that the thermal block 120 is cooled, and thereby the thermal The temperature control performance of the block 120 may be improved.

The temperature sensor 140 is installed in the thermal block 120 to measure the temperature and output a detection signal. For example, an LM35DT precision temperature sensor in the form of a TO-220 package can be used, but is not limited thereto, and various sensors for measuring temperature It goes without saying that can be used. LM35DT is a product of Texas Instruments and has an output characteristic of 10mV/℃. Therefore, an output voltage of 0.6V corresponds to a temperature of 60℃. Therefore, a temperature control target of the thermal block 120 by the controller 150, which will be described later, is to maintain the output voltage of the temperature sensor 140 at 0.6V (see FIG. 7).

The control unit 150 receives the detection signal from the temperature sensor 140 and controls the heating unit 130 and the heat dissipation fan 117 so that the thermal block 120 maintains a predetermined temperature or performs a predetermined thermal cycling. You can control the action. For example, if the thermal block 120 is below a predetermined temperature through the detection signal of the temperature sensor 140, the control unit 150 stops the heat dissipation fan 117 and operates the heating unit 130, and the temperature sensor ( When the thermal block 120 exceeds the predetermined temperature through the detection signal of 140), the heating unit 130 is stopped and the heat dissipation fan 117 is operated. At this time, the predetermined temperature of the thermal block 120 is Thermal cycling can be performed by running as determined in advance according to time. In addition, the control unit 150 replaces the opposite operation control of the heating unit 130 and the cooling fan 117, and controls the remaining operations under continuous operation of either the heating unit 130 or the cooling fan 117. It may also be configured to perform temperature control by controlling. Meanwhile, the controller 150 may be configured to vary the output of the heat dissipation fan 117 and the heat generator 130 in order to precisely control the temperature of the thermal block 120 as needed.

Of course, the controller 150 may be implemented in the form of a PCB 151 installed in the receiving space 111 as in the present embodiment. At this time, the control unit 150 may be configured to be made in the form of a single board such as the charging unit 161 and the communication module 180 to be described later.

4, 5 and 7, the controller 150 can be configured as an extremely simple feedback control temperature controller by using, for example, one operational amplifier in the form of an open-loop differential amplifier, and has an excellent constant temperature maintenance function. can have it In order to control the temperature of the thermal block 120, the control unit 150 inputs, for example, the output voltage of the temperature sensor 140 corresponding to the target temperature and the reference voltage of 0.6V to the inverting input terminal and the non-inverting input terminal of the operational amplifier, respectively. By differential comparison, if the reference voltage is higher than the voltage of the temperature sensor 140, that is, if the temperature of the thermal block 120 is lower than 60 ° C, a switch for supplying current to the heating unit 130 (here, a field effect transistor ) is turned on, and when the reference voltage is lower than the voltage of the temperature sensor 140, that is, when the temperature of the thermal block 120 is higher than 60 ° C, the switch that supplies current to the heating unit 130 is turned off. OFF control can be performed.

The control unit 150 may use a low-cost single-power OP-amp such as the 2904, and the open-loop voltage gain of this amplifier is about 10 ⁵ times or less. Here, if the operating voltage range is 10V, the open-loop voltage gain of 1 corresponds to 0.0001V. Therefore, on-off control is performed as described above in the range where the difference between the voltages of the two input terminals of the operational amplifier is 0.01V (corresponding to a temperature difference of 1 ° C) or more, but the voltage difference between the two input terminals is 0.001 V (corresponding to around 0.1 ° C). When it approaches, the open-loop voltage gain is only around 10, so it can operate like a proportional controller that supplies current to the heating unit 130 in proportion to the voltage difference, rather than on-off control. In addition, if the thermal impedance between the thermal capacity of the thermal block 120 and the heating resistance is appropriately adjusted, stable temperature control characteristics can be implemented like a proportional-derivative controller.

On the other hand, in such temperature control, since the heating unit 130 has only a heating function and cooling depends on the convective cooling of the thermal block 120, the time constant of temperature control becomes long, thereby reducing control robustness. there is In order to alleviate this, it is necessary to forcibly cool the thermal block 120 using the heat dissipation fan 117 . In this case, both a method of always operating the heat dissipation fan 117 and a method of constructing the same control loop having the opposite logic to that of the heating unit 130 can be applied, and there is no significant difference in temperature control performance between the two methods.

The power supply unit supplies power required for operation. The heating unit 130, the heat dissipation fan 117, the temperature sensor 140, and the control unit 150, as well as the communication module 180 and the display unit 210 to be described later. ), and to supply power required for operation of the display unit 220 and the like. The power supply unit can be used by converting the power supplied from the connector 170 through the cable 2 into power required for operation, for example, and as another example, the power of the battery 160 installed in the housing 110. can also be used.

The power supply unit may use an SMPS DC power supply, and at this time, the supply voltage may be 9 to 12V, and the supply current may be around 1A. This may be increased or decreased according to the required thermal capacity of the thermal block 120 .

The portable thermostat 100 for RNA amplification according to an embodiment of the present invention may include a connector 170 and a communication module 180.

The connector 170 is provided to be connected from the external terminal 1 to the cable 2. For example, a micro 5-pin USB connector is used, a C-type USB connector is used, or various other methods or structures are used. device can be used. This connector 170 serves to enable wired communication with the external terminal 1, but may also serve to receive power to be used by the power supply unit. Meanwhile, when the power supply uses the power of the battery 160, the connector 170 may serve to supply charging power to the battery 160. In this case, the battery 160 is charged by converting the power supplied through the connector 170 into charging power by the charging unit 161 and being supplied. The charging unit 161 may include, for example, a charging circuit unit.

The communication module 180 is provided to be connected by wireless communication with an external terminal 1, and is composed of a module enabling various wireless communications, such as Bluetooth communication with the external terminal 1 or Wi-Fi, for example. can Here, the terminal 1 may be a variety of information processing and communication devices, including, for example, a smart phone, tablet PC, desktop, or laptop computer, and may require installation and operation of applications or programs provided to implement required functions. .

The control unit 150 can control to perform an operation corresponding to an operation signal transmitted from the terminal 1 through the connector 170 or the communication module 180, where the operation signal turns on or Signals for selection of various operations, including off, pairing or connection for communication, setting of control temperature, and the like, may correspond.

The portable thermostat 100 for RNA amplification according to an embodiment of the present invention may further include a switching unit 190, a display unit 210, and a display unit 220.

The switching unit 190 is provided to input an operation signal to the outer surface of the housing 110, and may be composed of a single or multiple, on or off operation, pairing or connection for communication, and control. It is possible to perform operations for selecting various operations, including temperature setting.

The display unit 210 is provided on the outer surface of the housing 110 to display an operating state to the outside, and various display devices including an LCD may be used.

The display unit 220 is provided on the outer surface of the housing 110 to display the connection by the communication module 180 to the outside, for example, an LED or the like may be used.

The control unit 150 can control to perform an operation corresponding to the operation signal input by the switching unit 190, can control the display unit 210 to display the operating state, and the communication module 180 The display unit 220 can be controlled according to whether or not the is connected.

Referring to FIGS. 4 and 8, the portable thermostat 100 for RNA amplification according to the present invention has a light emitting unit installed on one side of the mounting groove 121 to emit light toward the Eppendorf tube 10 ( 230), and a light receiving unit 240 installed to receive light emitted from the light emitting unit 230 and passing through the Eppendorf tube 10 and provide a corresponding detection signal. Here, the light emitting unit 230 may be, for example, an LED, and the light receiving unit 240 may be a photodiode or color sensor for detecting the amount of light and the color of light. The control unit 150 receives the detection signal from the light receiving unit 240, measures the change in absorbance during the amplification and color development reaction period in the Eppendorf tube 10, and displays the display unit 210, wire cable, or communication module 180. ) can be provided to the external terminal 1 through. The controller 150 may be configured to measure absorbance or scattering of fluorescent light along with temperature and time in order to measure absorbance change.

The portable thermostat 100 for RNA amplification according to the present invention is installed on one side of the mounting groove 121 and measures the fluorescent light emitted from the fluorochrome reaction in the Eppendorf tube 10 to obtain a corresponding detection signal. It may further include a fluorescence receiving unit 250 that provides. The control unit 150 receives the detection signal of the fluorescence receiver 250, measures the reaction of the fluorescent dye in the Eppendorf tube 10, and detects the external It can be provided to the terminal (1).

The portable thermostat 100 for RNA amplification according to the present invention is provided on the PCB 151 in the housing 110 and includes an embedded switch for switching to select various diagnostic reagents customized constant temperature amplification and thermal cycling. A selection unit 260 such as may be further included. At this time, the control unit 150 receives the switching signal selected by the selector 260 and controls the operation of the heating unit 130 and the heat dissipation fan 117 to perform constant temperature or thermal cycling corresponding to the switching signal. You can control it.

According to the portable thermostat for RNA amplification according to the present invention, while having a very simple structure and drastically reducing the size, by inserting an Eppendorf tube and maintaining the temperature around 60 ° C, RNA amplification is achieved. It can be made, and because of this, it can be used for PCR thermocycling techniques.

In addition, according to the present invention, the temperature maintaining performance is excellent, the portability is simple, and the purchase cost is low, so that economical use is possible, and thus, it can be used as an inexpensive self-diagnosis kit.

Although the present invention has been described with reference to the accompanying drawings, various modifications may be made without departing from the spirit of the present invention. Therefore, the scope of the present invention should not be limited to the above-described embodiments and should not be defined, but should be defined by the claims and equivalents of these claims.

1: terminal 2: cable
10: Eppendorf tube 110: housing
111: receiving space 112: opening
113: fixed boss 114: opening and closing part
115: cover 116: vent
117: heat dissipation fan 120: thermal block
121: mounting groove 122: fixed rib
123: fixing bolt 130: heating part
140: temperature sensor 150: control unit
151: PCB 160: battery
161: charging unit 170: connector
180: communication module 190: switching unit
210: display unit 220: display unit
230: light emitting unit 240: light receiving unit
250: fluorescence receiving unit 260: selection unit

Claims (8)

a housing having an accommodation space formed therein, an opening opening upward, a ventilation hole provided, and a cover provided to open and close the opening;
a thermal block installed in the accommodating space, having at least one mounting groove for mounting an Eppendorf tube open upward on a side exposed through the opening, and made of a thermally conductive material;
a heating unit provided to supply heat to the Eppendorf tube by providing heat to the thermal block;
a heat dissipation fan installed to discharge heat from the thermal block within the accommodation space through the ventilation hole by means of a blowing force;
a temperature sensor installed to measure the temperature of the thermal block and output a detection signal;
a control unit that receives a detection signal from the temperature sensor and controls operations of the heating unit and the heat dissipation fan so that the thermal block maintains a predetermined temperature or performs predetermined thermal cycling;
The thermal block,
Fixing ribs are provided at the lower end so as to protrude horizontally to both sides, and each of the fixing ribs is fixed to a fixing boss extending downward from both sides of the opening in the accommodation space with a fixing bolt, so that it is fixed to be exposed to the opening, It is installed to be spaced apart from the bottom surface of the accommodation space; and
a power supply unit supplying power necessary for operation;
Containing, portable thermostat for RNA amplification. The method of claim 1,
The thermal block,
A portable constant-temperature device for RNA amplification, wherein a plurality of mounting grooves are formed to open upward in a vertical direction, the heating unit is attached to one side, and the temperature sensor is attached to the other side. The method of claim 1,
A connector provided to be connected to a cable from an external terminal; and
It further includes; a communication module provided to be connected by wireless communication with an external terminal;
The control unit,
A portable constant temperature device for RNA amplification, which is controlled to perform an operation corresponding to an operation signal transmitted from the terminal through the connector or the communication module. The method of claim 3,
a switching unit provided to input an operation signal to an outer surface of the housing;
a display unit provided on an outer surface of the housing to display an operating state to the outside; and
A display unit provided on the outer surface of the housing to externally display the connection by the communication module; further comprising,
The control unit,
Portable RNA amplification for controlling the operation corresponding to the operation signal input by the switching unit, controlling the display unit to display the operating state, and controlling the display unit according to whether the communication module is connected or not. thermostat. The method according to any one of claims 1, 3 and 4,
The control unit,
In order to control the temperature of the thermal block, the output voltage of the temperature sensor corresponding to the target temperature and the reference voltage of 0.6V are inputted to the inverting input terminal and the non-inverting input terminal of the operational amplifier, respectively, and differentially compared, so that the reference voltage is the temperature When the voltage of the sensor is higher than that, the switch for supplying current to the heating unit is turned on, and when the reference voltage is lower than the voltage of the temperature sensor, the switch for supplying current to the heating unit is turned off. , A portable thermostat for RNA amplification. The method according to any one of claims 1, 3 and 4,
a light emitting unit installed at one side of the mounting groove to emit light toward the Eppendorf tube; and
A light receiving unit installed to receive light emitted from the light emitting unit and passing through the Eppendorf tube and provide a corresponding detection signal;
The control unit,
A portable constant temperature device for RNA amplification, which receives the detection signal from the light receiver, measures the absorbance change during the amplification and color development reaction period in the Eppendorf tube, and provides it to a display unit or an external terminal. The method according to any one of claims 1, 3 and 4,
A fluorescence receiver installed on one side of the mounting groove and providing a corresponding detection signal by measuring the fluorescence light emitted by the reaction of the fluorescence dye in the Eppendorf tube;
The control unit,
A portable constant temperature device for RNA amplification, which receives a detection signal from the fluorescence receiver, measures a fluorescence dye reaction in the Eppendorf tube, and provides the result to a display unit or an external terminal. The method according to any one of claims 1, 3 and 4,
Further comprising a selection unit provided on the PCB in the housing and switching to select various diagnostic reagents customized constant temperature amplification and thermal cycling,
The control unit,
A portable constant temperature device for RNA amplification, which receives a switching signal selected by the selection unit and controls operations of the heating unit and the heat dissipation fan to perform constant temperature or thermal cycling corresponding to the switching signal.

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