KR20230011001A - A composition for moisturizing the skin comprising the extract of Equisetum hyemale L. as an active ingredient - Google Patents

Abstract

The present invention relates to a composition for moisturizing the skin comprising an Equisetum hyemale L. extract as an active ingredient and, more specifically, to a composition which does not irritate the skin and has an excellent skin moisturizing effect. The composition according to the present invention can be provided as a cosmetic composition which does not irritate the skin when applied to the skin, thereby not causing skin problems. In addition, the composition can be used even if users have environmental skin diseases by including ingredients derived from natural substances, and can exhibit an excellent skin moisturizing effect. Also, the composition can be provided as a food composition, and thus can exhibit a skin moisturizing effect by ingestion.

Description

A composition for moisturizing the skin comprising the extract of Equisetum hyemale L. as an active ingredient}

The present invention relates to a composition for moisturizing skin containing a horse chestnut extract as an active ingredient, and more particularly, to a composition having no skin irritation and excellent skin moisturizing effect.

The skin plays a very important role as a barrier function that protects the object from the outside. The barrier function is a protective function that protects against various stimuli from the outside (chemical substances, air pollutants, dry environment, ultraviolet rays, etc.) and prevents excessive release of moisture in the body through the skin. This protective function is the stratum corneum composed of keratinocytes. It can maintain its function only if it is formed normally.

The outermost stratum corneum (horney layer) of the epidermis is formed from keratinocytes and is composed of fully differentiated keratinocytes and a lipid layer surrounding them (Marcelo C. L. et al, J. Invest. Dermatol., 80, pp37-44, 1983).

Keratinocytes are characteristic cells in which basal cells continuously proliferating in the lowermost layer of the epidermis go through morphological and functional changes step by step and rise to the skin surface. After a certain period of time, old keratinocytes are removed from the skin. It is eliminated and new keratinocytes take over its function, and this repetitive series of changes is called “differentiation of epidermal cells” or “keratinization”.

In addition, during the keratinization process, keratinocytes form the stratum corneum while producing natural moisturizing factor (NMF) and intercellular lipids (ceramide, cholesterol, fatty acid), making the stratum corneum firm and flexible, thereby improving the skin barrier (skin barrier). function as a barrier).

This stratum corneum can easily lose its function due to lifestyle factors such as excessive face washing or bathing, environmental factors such as dry air pollutants, and endogenous diseases such as atopic skin or senile skin. Recently, due to various factors that have increased in recent years, the number of parts complaining of dry skin symptoms and various disorders caused by them is gradually increasing.

Therefore, in order to maintain proper skin moisture, various studies have been conducted to supply moisture from the outside or to prevent moisture loss from the body, and various moisturizers having the ability to actually retain moisture have been developed.

As a skin moisturizer, it is common to use substances that can increase water retention in the stratum corneum, such as lipid components such as ceramide, essential fatty acids and lipid complexes (Rawlings A. V. et al, J. Invest. Dermatol., 5, pp731- 741, 1994).

However, recently, the risk factors for the skin are gradually increasing, the rate of formation and exfoliation of the stratum corneum is slowed due to changes in dietary habits, and the amount of moisturizing factors and lipids in the stratum corneum is reduced due to the decline in the function of keratinocytes, so that the stratum corneum is normal. There is an increasing number of people with skin that does not function as a skin barrier.

Conventional moisturizers have a property of absorbing moisture or a function of preventing moisture evaporation, and have been used in a way to increase moisture retention in the stratum corneum. .

On the other hand, the frequency of occurrence of various environmental skin diseases such as atopic dermatitis and contact dermatitis has recently been rapidly increasing. Atopic dermatitis is a skin abnormality syndrome in which symptoms such as strong itching of unknown cause, dryness, severe exfoliation, skin cracking, and erythema appear in combination.

The skin with environmental skin diseases such as atopic dermatitis needs to be continuously and sufficiently moisturized, as well as to heal skin diseases and improve skin.

It is necessary to develop a product for moisturizing the skin that has excellent skin moisturizing effect and does not cause skin irritation, so there is no inconvenience in use.

KR 10-2015-0086922 A1

An object of the present invention is to provide a composition for moisturizing skin containing a horse chestnut extract as an active ingredient, which is free from skin irritation and has excellent skin moisturizing effect.

Another object of the present invention is provided as a cosmetic composition, which does not cause skin irritation when applied to the skin, does not cause skin trouble, and can be used even when having environmental skin diseases by including components derived from natural substances such as horsetail extract. It is possible and to provide a composition capable of exhibiting an excellent skin moisturizing effect.

Another object of the present invention is to provide a composition that is provided as a food composition and can exhibit a skin moisturizing effect by ingestion.

In order to achieve the above object, the composition for moisturizing the skin according to an embodiment of the present invention includes horsetail extract as an active ingredient.

The composition has no skin irritation and has an excellent skin moisturizing effect.

The extract is extracted by using any one selected from the group consisting of water, lower alcohols having 1 to 6 carbon atoms, and mixtures thereof as an extraction solvent.

The composition may further include a fermented seaweed extract.

A cosmetic composition for moisturizing the skin according to another embodiment of the present invention includes the composition for moisturizing the skin.

The cosmetic composition may be a formulation selected from the group consisting of skin, serum, lotion, emulsion, cream, essence, lotion, foundation, pack, soap, surfactant-containing cleansing, spray, and powder.

Functional food composition for moisturizing the skin according to another embodiment of the present invention includes the composition for moisturizing the skin.

Hereinafter, the present invention will be described in more detail.

In the human body, the skin is an organ that functions as a protective film that protects the body from the external environment, and plays a role in preventing the loss of biological components, such as water and electrolytes, and preventing harmful substances from entering the body from the outside. do.

Such skin is largely divided into an epidermal layer, a dermal layer, and a subcutaneous fat layer. The epidermal layer is composed of keratinocytes and melanocytes. The outermost keratinocyte layer of the skin directly contacts the external environment, so it requires strong resistance to physical, chemical or substance permeation, and prevents loss of moisture from the body to the outside. At the same time as zooming, it should maintain its flexibility by retaining adequate moisture in itself.

In general, the moisture content of the skin is about 70% in the dermal layer, but decreases toward the epidermal layer, and becomes about 10 to 30% in the keratinocyte layer. The water supplied from the dermal layer moves to the top of the stratum corneum mainly by passive diffusion and is finally discharged to the outside.

This is called Trans Epidermal Water Loss (TEWL), and it is known that sebum and epidermal lipids, which are lipid film components of the keratinocyte layer, maintain this transepidermal water loss at an appropriate level. In addition, it is known that a substance having a hydrophilic water-holding ability called Natural Moisturizing Factor (NMF) exists in the keratinocyte layer and plays an important role in moisturizing the skin.

When the moisture of the normal keratinocyte layer is maintained at about 10 to 30%, the skin is smooth and soft, and the body protective function is normally exhibited. However, when the moisture content of the keratinocyte layer is less than 10%, the skin becomes rough and loses the body's protective function, resulting in aging.

For example, in the case of dry skin, the cohesion of keratinocytes is weakened, resulting in a scaling phenomenon in which keratinocytes are peeled off like scales from the skin surface. This is because it is less than normal skin.

In addition, even healthy skin may suffer from moisture deficiency due to harsh external environments, such as wind, cold weather, sunlight, face washing, and shaving, resulting in poor skin condition.

Therefore, it is very important to properly maintain the moisture content of the keratinocyte layer of the skin. It is known that when the moisture content of the stratum corneum is less than 10%, the skin becomes dry and causes skin trouble. The amount of water in the stratum corneum produced by regular keratinization always maintains 10 to 20%.

Currently, an occlusive agent is used to maintain moisture in the skin. The occlusive agent is a hydrophobic substance that promotes water retention by forming a barrier on the skin to prevent water loss.

The most commonly used occlusive agents include petrolatum, lanolin, cocoa butter, mineral oil and silicones. In addition, to make cosmetics, 20 to 50 different types of raw materials are generally used, such as sodium lauryl sulfate, dimethyl sulfoxide, croton oil, and sodium hydroxide. When these raw materials are applied to products, they can cause irritation such as inflammation, itching, and allergies to the skin.

The main feature of the composition of the present invention is that it does not irritate the skin when applied to the skin and exhibits an excellent skin moisturizing effect.

The composition for moisturizing the skin according to an embodiment of the present invention includes horsetail extract as an active ingredient.

The horsetail (Equisetum hyemale L.) is an evergreen perennial plant growing in wetlands of mountain valleys, with a height of 30 to 60 cm, a stem that seems to grow, has no branches, and has 1018 ridges between nodes. The degenerated scale-like leaves are attached to each other and completely surround the node to form a leaf sheath, and the tip is serrated and runs alternately with each ridge. The sporangia hangs at the end of the main stem, has a conical shape, and has a sharp end. The stock is also called the purpose, and the above-ground part is collected from summer to autumn, dried in the shade, and used as a medicine.

The extract may be extracted using any one selected from the group consisting of water, lower alcohols having 1 to 6 carbon atoms, and mixtures thereof as an extraction solvent.

Specifically, pulverizing the dried horseradish; leaching the pulverized product using an organic solvent; drying the sample after leaching; re-leaching the dried sample using an organic solvent; drying the sample after leaching; leaching with water; And including the step of leaching, it is possible to obtain horse chestnut extract.

The step of fractionating the horse chestnut extract extracted using the organic solvent using an organic solvent may be further included.

A method for preparing the extract may be a conventional extraction method in the art, such as an ultrasonic extraction method, a leaching method, and a reflux extraction method. Specifically, it may be an extract obtained by extracting a natural product from which foreign substances are removed by washing and drying with water, alcohol having 1 to 6 carbon atoms, or a mixed solvent thereof, or may be an extract extracted by sequentially applying the solvents to a sample.

The reflux extraction method is based on 100 mL of alcohol having 1 to 6 carbon atoms, 10 to 30 g of pulverized natural product, 1 to 3 hours of reflux, and 50 to 100% of alcohol having 1 to 6 carbon atoms. More specifically, based on 100 mL of alcohol having 1 to 6 carbon atoms, 10 to 20 g of pulverized natural product, 1 to 2 hours of reflux, and 70 to 90% of alcohol having 1 to 4 carbon atoms.

The leaching method is by 15 to 30 ℃, for 24 to 72 hours and 50 to 100% of alcohol having 1 to 6 carbon atoms. More specifically, at 20 to 25° C. for 30 to 54 hours and 70 to 80% of an alcohol having 1 to 6 carbon atoms.

The ultrasonic extraction method is by 30 to 50 ℃, 0.5 to 2.5 hours and 50 to 100% of alcohol having 1 to 6 carbon atoms. Specifically, at 40 to 50° C. for 1 to 2.5 hours and 70 to 80% of an alcohol having 1 to 6 carbon atoms.

The extraction solvent may be used in an amount of 2 to 50 times, more specifically, 2 to 20 times, based on the weight of the sample. For extraction, the sample may be left for leaching in the extraction solvent for 1 to 72 hours, more specifically 24 to 48 hours.

After extraction, the extract can be fractionated by sequentially applying a fresh fractionation solvent. The fractionation solvent used in the fractionation is at least one selected from the group consisting of water, hexane, butanol, ethylacetic acid, ethyl acetate, methylene chloride, and mixtures thereof, and is preferably ethyl acetate or methylene chloride.

After obtaining the extract or fraction, a method such as concentration or lyophilization may be additionally used.

The composition may further include a fermented seaweed extract. The skin moisturizing composition may be provided as a moisturizing composition having an excellent moisturizing effect and a high preference even when it contains only the horsetail extract, but may exhibit an increased moisturizing effect by using a mixture with a fermented seaweed extract, and a highly desirable cosmetic composition or It enables provision as a food composition.

In addition, when the seaweed extract is used as it is, the fermented seaweed extract has a problem in that the preference is lowered due to the peculiar odor of seaweed. In order to prevent this problem, when an active ingredient is extracted from fermented seaweed and mixed with horse chestnut extract and used, it is possible to provide a composition with high preference as well as enhanced moisturizing effect.

The seaweed extract is specifically, seaweed extract, seaweed extract, capricorn extract, seaweed extract, fusiformis extract, wakame extract, laver extract, kelp extract, Ecklonia cava extract, agar-agar extract, hearing extract, chlorella extract, seaweed extract, semogasari extract, rhubarb It is selected from the group consisting of extracts and mixtures thereof, and preferably may be selected from the group consisting of sea string extract, semogasari extract, and mixtures thereof, but is not limited to the above examples.

Gracilaria verrucosa is a type of red seaweed that belongs to the family Dolphinidae and grows attached to stones and shells in intertidal zones such as Korea, Japan, Sakhalin Island, and Taiwan. In particular, it breeds on pebbles or piles of shallow beaches where river water flows into the sea, and also grows on reefs in the open sea. The body is gathered from a small tray-shaped root and has a cylindrical shape. It is densely branched into a feather shape, and the branches are biased to one side. Branches protruding from the body are cartilaginous, wire-shaped with a diameter of less than 1 mm, blackish purple or dark brown, 1-3 mm in diameter, and grow up to 2-3 m in cylinder. It is also used as a raw material.

The triangle (Gloiopeltis furcate) is a reddish-brown annual seaweed, meaning that the stem is thin in the middle of the furgasari. Samogasari is 5 to 15 cm high, the body is cylindrical, and the branches split a little irregularly, and the body shape varies greatly depending on where it grows. The branches are hollow, the branches are constricted, and the width of the branches reaches 4mm. Semogasari grows profusely throughout winter and spring, matures in early summer, and produces spores.

The fermented seaweed extract is subjected to a fermentation aging process by putting the washed seaweed into a fermentation vessel and inoculating microorganisms thereon.

The microorganisms are Lactobacillus salivarius, Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus rhamnosus, Lactobacillus plantarum, lactobacillus Bacillus helveticus, Lactobacillus fermentum, Lactobacillus paracasei, Lactobacillus casei, Lactobacillus delbrueckii, Lactobacillus reuteri ( Lactobacillus reuteri), Lactobacillus buchneri, Lactobacillus gasseri, Lactobacillus johonsonii, Lactobacillus kefir, Lactobacillus lactis, Lactococcus lactis), Lactococcus plantarum, Lactococcus raffinolactis, Enterococcus faecalis, Enterococcus faecium, Streptococcus thermophilus thermophilus), Leuconostoc lactis, Leuconostoc mesenteroides, etc. Lactobacillus and Bifidobacterium animals, Bifidobacterium bifidum, Bifidobacterium brev e), Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium pseudolongum, Bifidobacterium themophilum, Bifidobacterium It may include bifidobacteria such as Bifidobacterium adolescentis, more preferably Lactobacillus casei, Lactobacillus rhamnosus, Bifidobacterium bifidum ) It may be selected from the group consisting of Bifidobacterium, Bifidobacterium breve, Bifidobacterium, and Lactobacillus acidophilus, but is not limited to the range of the above microorganisms and can be used without limitation. .

After inoculating the microorganism, a fermentation aging process is performed at 30 to 35 ° C. for 1 to 2 days. After the fermentation and aging process is completed, the fermented seaweed extract is prepared by the extract preparation process described above.

The composition may include 20 to 40 parts by weight of the fermented sea bream extract and 20 to 40 parts by weight of the fermented semogasari extract, based on 100 parts by weight of the horsetail extract. When mixed and used within the above range, skin irritation does not occur, the moisturizing effect is relatively improved, and skin application properties can be improved.

A cosmetic composition for moisturizing the skin according to another embodiment of the present invention includes the composition of the present invention.

The cosmetic composition of the present invention may be prepared by mixing a dermatologically acceptable carrier in addition to the composition of the present invention.

The dermatologically acceptable carrier may include purified water, oil, wax, fatty acid, fatty alcohol, fatty acid ester, surfactant, moisture absorbent, thickener, antioxidant, viscosity stabilizer, chelating agent, buffer, preservative, lower alcohol, and the like. However, it is not limited thereto, and the type and concentration are various, and include parts that can be changed by those skilled in the art within the scope of the present invention.

In addition, if necessary, functional substances such as whitening agents, moisturizing agents, anti-inflammatory agents, antibacterial agents, antifungal agents, vitamins, sunscreens, antibiotics, anti-acne agents, perfumes, and dyes may be included, which are generally used in the cosmetic field. As may be included in the cosmetic composition according to the present invention. The cosmetic composition of the present invention may contain at least one moisturizing active ingredient exhibiting the same or similar function in addition to the composition of the present invention in order to enhance its functional effect.

Cosmetics prepared from the cosmetic composition of the present invention can be prepared in the form of general emulsified formulations and solubilized formulations. Emulsified cosmetics include nutrition lotion, cream, essence, etc., and solubilized cosmetics include softening lotion.

In addition to the composition of the present invention, the cosmetic may be prepared in the form of an adjuvant that can be applied topically or systemically, which is commonly used in the art, by containing a dermatologically acceptable medium or base.

Suitable cosmetic formulations include, for example, solutions, gels, solid or pasty anhydrous products, emulsions obtained by dispersing an oil phase in an aqueous phase, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes), non-ionic forms. It may be provided in the form of a follicular dispersion, cream, toner, lotion, powder, ointment, spray or conceal stick. In addition, it can be prepared in the form of a foam (foam) or the form of an aerosol composition further containing a compressed propellant.

Products to which the cosmetic composition of the present invention can be added include, for example, cosmetics such as astringent lotion, softening lotion, nutrient lotion, various creams, essences, packs, foundations, etc., and cleansing, face wash, soap, treatment or beauty essence etc. Specific formulations include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrient lotion, massage cream, nutrient cream, moisture cream, hand cream, essence, nutrient essence, pack, soap, shampoo, cleansing Formulations such as foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, loose powder or eye shadow are included.

A food composition according to another embodiment of the present invention includes the composition for moisturizing the skin.

At this time, the amount of the extract in the food or beverage may be added at about 0.01 to 15% by weight of the total food weight, and the health drink composition may be added at a rate of about 0.01 to 10g based on 100 ml, but is not limited thereto .

When the food composition of the present invention is a beverage composition, there is no particular limitation on other ingredients except for containing the extract as an essential ingredient in the indicated ratio, and it may contain various flavoring agents or natural carbohydrates as additional ingredients like conventional beverages. can Examples of the aforementioned natural carbohydrates include monosaccharides such as glucose, fructose, and the like; disaccharides such as maltose, sucrose and the like; and polysaccharides such as conventional sugars such as dextrins, cyclodextrins, and the like, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (thaumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) may be used, but are not limited thereto. The ratio of the natural carbohydrates may generally range from about 1 to about 20 g per 100 ml of the composition of the present invention, but may not be limited thereto.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and enhancers (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its Salts, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages, and the like may be included, but may not be limited thereto.

In addition, the composition of the present invention may contain natural fruit juice and fruit flesh for producing fruit juice beverages and vegetable beverages, and these components may be used independently or in combination. The ratio of these additives is not critical, but may be selected in the range of about 0.1 to about 20 parts by weight per 100 parts by weight of the composition of the present invention, but is not limited thereto.

A functional food composition according to another embodiment of the present invention may include the composition for moisturizing the skin.

The functional food referred to in the present invention is a food manufactured and processed by extracting, concentrating, refining, mixing, etc., a specific ingredient as a raw material or a specific ingredient contained in a food raw material for the purpose of health supplementation, and includes health supplement food. In addition, it is a food designed and processed to sufficiently exert the biological control functions of food components, such as bio-defense, regulation of biological rhythm, prevention and recovery of disease, etc., with functions related to disease prevention and recovery. make it all inclusive.

The present invention relates to a composition for moisturizing skin containing horsetail extract as an active ingredient, which does not cause skin irritation and has excellent skin moisturizing effect. It is provided as a cosmetic composition and does not cause skin irritation when applied to the skin. It does not cause occurrence, can be used even when having environmental skin diseases, including components derived from natural substances, and can exhibit an excellent skin moisturizing effect.

In addition, provided as a food composition, it can exhibit a skin moisturizing effect by ingestion.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement the present invention. However, the present invention may be embodied in many different forms and is not limited to the embodiments described herein.

manufacturing example

Preparation of horsetail extract

After obtaining only the aerial part of Horseshoe, it was washed, dried, pulverized to prepare a pulverized product, and freeze-dried. 1 mg of 3 g of freeze-dried horsetail was put into a 15 ml tube, and 10 ml of 70% ethanol was added again. The samples were mixed and extracted for 18 hours in a rounded mixing machine. After extraction, only the supernatant was collected after centrifugation at 3000 rpm for 10 minutes. Ethanol (ethanol) contained in the collected supernatant was evaporated at 40 ℃. And the remaining samples were lyophilized. Horseradish extract (EE) used in this experiment was extracted with a yield of 11.5% in the horseradish raw material.

Preparation of fermented seaweed extract

After drying the string, it was pulverized using a grinder. Lactobacillus rhamnosus was inoculated into the pulverized sea string and fermented at 25 ° C. for 3 days to prepare fermented sea string. Afterwards, a fermented hairpin extract (GE) was prepared in the same manner as in the preparation method of the horse chestnut extract.

The fermented semogasari extract (FE) was prepared using the same method as the fermented hairpin extract.

Preparation of mixed extract

Horseradish extract (EE), fermented hairpin extract (GE), and fermented semogasari extract (FE) were mixed and used as shown in Table 1 below.

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Comparative Example 1 Comparative Example 2 EE 100 - - 100 100 100 100 100 GE - 100 - 20 30 40 10 50 FE - - 100 20 30 40 10 50

(unit weight parts)

Experimental Example 1

skin irritation test

The irritation relieving effect was confirmed through a human skin patch test on 15 women in their 20s and 30s who had no past history of hypersensitivity to skin irritation and no current skin disease or skin allergy symptoms. Examples and comparative examples were used in which 1.0% by weight of lactic acid, a skin irritant, was added as an irritant.

First, after washing with 70% by weight of ethanol for the participant's test, it was dried. 15 μg of the prepared test substance was dropped into a fin chamber (Finn chamber, 100x10, EPITEST, Finland), and then sealed patched on the inside of the forearm of the test subject. After applying the patch for 24 hours and removing the patch, the test site was marked with a marking pen. After 1 hour and 24 hours after marking, the test site was observed using a magnifying glass (8MC-150, DAZOR, USA) to observe the presence or absence of erythema and edema.

Skin reactions were determined according to the regulations of the International Contact Dermatitis Research Group (ICDRG) in Table 2, and the results are shown in Table 3.

sign score Evaluation standard - 0 unresponsive ± 0.5 faint or mild erythema + One Well-circumscribed but mild erythema, edema, and papules ++ 2 Marked erythema, papules and vesicles +++ 3 Severe erythema and blisters, scab formation

[Equation 1]

Mean response rate = (score x number of responses x 100 x 1/2)/[3 (maximum score) x total number of subjects (n)]

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Comparative Example 1 Comparative Example 2 Reactivity (%) 3.10 3.85 4.10 2.55 2.45 2.35 5.75 6.25

According to the above experimental results, it was confirmed that skin irritation did not occur when using the horsetail extract, and severe skin irritation did not occur even when using the fermented hairpin extract and the fermented semogasari extract. However, in the case of the complex extract, when mixed and used within a specific range, it was confirmed that the best skin irritation relief effect was exhibited when used due to the synergistic effect of the mixed use between the extracts.

Experimental Example 2

Moisturizing effect experiment

Measurement of water holding capacity The water holding capacity was measured by using a Corneometer (Courage + Khazaka, GmbH, Germany) to measure the electrical conductivity of the skin surface. A certain amount (0.03g/16cm ² ) of each cosmetic prepared in Comparative Example was applied to the inside of the lower chest of 15 test subjects, and the moisture content of the skin before application and 2 hours after application was measured. Normal skin without any treatment was used as a control group. was used as The results are shown in Table 4 below.

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Comparative Example 1 Comparative Example 2 before application 50 50 50 50 50 50 50 50 2 hours later 72 65 67 84 86 85 53 54

According to the above experimental results, it was confirmed that when the horse chestnut extract was used, an excellent skin moisturizing effect appeared even after 2 hours, and when used as a complex extract, an excellent skin moisturizing effect appeared within a specific range.

Experimental Example 3

Skin applicability evaluation

Cosmetic creams were prepared using the compositions of Examples and Comparative Examples, and their application properties were evaluated.

3% by weight of each of the compositions of Examples and Comparative Examples, 1% by weight of ascorbic acid-2-magnesium phosphate, 1% by weight of water-soluble collagen (1% aqueous solution), 0.01% by weight of sodium citrate, 0.05% by weight of citric acid, 1,3- A cream was prepared by mixing 3% by weight of butylene glycol and the remaining purified water, and 6 products were provided to 15 women in their 20s and 30s, and then a relative evaluation of skin applicability was requested. Evaluation was requested on a scale of 1 to 5, and the higher the score, the better the product.

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Comparative Example 1 Comparative Example 2 skin spreadability 3 2 2 4 5 4 One 2

According to the evaluation results, the spreadability of the cream containing only the horse chestnut extract was evaluated to be moderate, but when the complex extract was used, the spreadability was very good, increasing the possibility of providing a cream with high preference.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements of those skilled in the art using the basic concept of the present invention defined in the following claims are also made according to the present invention. falls within the scope of the rights of

Claims (7)

Contains horse chestnut extract as an active ingredient
A composition for moisturizing the skin. According to claim 1,
The composition has no skin irritation and has an excellent skin moisturizing effect.
A composition for moisturizing the skin. According to claim 1,
The extract is extracted by using any one selected from the group consisting of water, lower alcohols having 1 to 6 carbon atoms, and mixtures thereof as an extraction solvent.
A composition for moisturizing the skin. According to claim 1,
The composition further comprises a fermented seaweed extract
A composition for moisturizing the skin. Claims 1 to 4, comprising the skin moisturizing composition according to any one of the preceding
A cosmetic composition for moisturizing the skin. According to claim 5,
The cosmetic composition is a formulation selected from the group consisting of skin, serum, lotion, emulsion, cream, essence, lotion, foundation, pack, soap, surfactant-containing cleansing, spray and powder
A cosmetic composition for moisturizing the skin. Claims 1 to 4, comprising the skin moisturizing composition according to any one of the preceding
Functional food composition.