KR20220134080A - An aquarium fish feed composition comprising hermetia illucens and sacha inchi and its preparing method - Google Patents

Abstract

The present invention relates to an aquarium fish feed composition comprising Hermetia illucens and Plukenetia volubilis nuts, and more specifically, to an aquarium fish feed composition which does not use fishmeal, which was previously used as a main ingredient of feed, but uses Hermetia illucens and Plukenetia volubilis nuts in a predetermined ratio, and thus can prevent fish diseases such as white spot. The aquarium fish feed composition according to the present invention has effects of increasing crude protein and preventing white spot disease compared to existing fish meal feeds, and also exhibits remarkably excellent effects in various feed characteristics such as floating properties, water pollution degree, Color development and luminescent properties, fry survival rate, etc.

Description

An aquarium fish feed composition comprising hermetia illucens and sacha inchi and its preparing method

The present invention relates to an ornamental fish feed comprising Dongaebackae and omega nut, and more particularly, by replacing the previously used fishmeal and mixing Dongaebackae and omega nut in a predetermined ratio, an increase in nutrients such as crude protein and fish such as white point disease It relates to a feed for ornamental fish that can suppress diseases occurring in

In Korea, white spot disease started to occur suddenly in fish farms and livestock farms from around 2005, and recently, it has been transmitted to a point where it is almost impossible to use. is experiencing serious difficulties.

In addition, the decrease in the production of fishmeal used in fish feed and the simultaneous price increase have caused serious concerns about the need for alternative raw materials used for feed. This frequent occurrence is emerging as a major problem.

In particular, in the case of ornamental fish, it is known that the main causes of problems such as decreased immunity and disease occurrence during the fish farming process are high-density breeding among intensive large-scale production methods, excessive use of pharmaceuticals, and increased use of chemical treatment agents for feed materials. Failure to prevent diseases of ornamental fish or low resistance to diseases can cause great damage to farms' productivity and economy.

Therefore, various efforts such as antibiotics or vaccine therapy have been attempted to increase resistance to disease in aquaculture, but the effect is insignificant. As a result, it brought many problems, such as a decrease in domestic productivity and the collapse of a small company. In other words, in order to help directly increase the productivity of aquaculture by solving problems such as improving the growth rate of ornamental fish, reducing mortality and reducing treatment costs, the development of alternative antibiotics, immune enhancers, functional additives, and metabolic promoters is essential in the domestic ornamental fish business area. which is required as

In addition, the Ministry of Agriculture, Food and Rural Affairs decided to completely ban the addition of antibiotics in compound feeds from July 2011. However, the replacement efficiency of currently developed alternatives to antibiotics is only 50%, and it is urgent to develop an alternative comprehensive technology after regulating antibiotics.

Ornamental fish (觀賞魚) are divided into goldfish, koi, freshwater fish, tropical fish, and saltwater fish. In particular, as tropical fish and saltwater fish depend on high-priced imports, attention should be paid to water quality maintenance and disease prevention. Due to the lack of secretion of mucus, which is the protective film of the body, it is easy for bacteria to penetrate into the body through the tip of the fin, which is the weakest part of the body, causing various diseases and abnormalities in the heart system. In particular, among fish diseases, white spot disease (ichthyophthiriasis) is a disease in which the body of a fish is covered with white spots. White spot disease is one of the typical diseases that occur in ornamental fish, and it is caused by parasitic worms, which are a kind of ciliate, Ichthyophthirius multifilii s, which penetrates the skin of fish and parasitizes them. Freshwater fish worms have long been known as major pathogenic parasites in temperate and tropical fish worldwide. White point worm has no host specificity, so it is parasitic on almost all freshwater fish, and has strong infectivity to aquaculture and ornamental fish, causing mass death within a short period of time, causing great damage, and is one of the important parasites that cause a lot of economic loss (Mathews, R. A. Parasitic Diseases of Fish, pp. 17-42, Tresaith, UK: Samara Publishing, 1994), known as a chronic disease that occurs frequently throughout the year (Boyoung Ji et al. 1996. Characteristics of the developmental stage of Ichthyophthirius multifiliis . Korean disease Journal, 9, 21).

When white spot disease occurs, the damage is severe, but it is difficult to select an appropriate treatment drug, and even if it has been rescued by a treatment drug, it will spread again at a certain time and cause damage. Chemotherapy (Luzardo-A''lvarez, A. et al. 2003. Oral pharmacological treatments for Ichthyophthiriosis of rainbow trout ( Oncorhynchus mykiss ). Aquaculture, 220, 15; Paivi R. et al. 2005. Treatment of ichthyophthiriasis after malachite green. I. Concrete tanks at salmonid farms. Dis. Aquat. Org., 64, 69) and plant extracts [Ekanem, AP et al. 2004. Effects of extracts of Mucana pruriens (Fabaceae) and Carica papaya (Caricaceae) against the protozoan fish parasite Ichthyophthirius multifiliis. Parasitol. Res., 92, 361; Jindo Kim et al. 2011. In vitro extermination effect of white spot insect by extracts of three types of plants. There are reports using the Journal of the Korean Society for Disease Control and Prevention, 24, 289]. However, at present, clear measures for the prevention and treatment of white spot disease have not yet been established in Korea, and formalin for fisheries is known as the only drug to prevent mass death.

As a conventional technology for producing a feed that is resistant to diseases of fish, Korean Patent Application Laid-Open No. 1998-086536 proposes a natural feed additive for fish containing aloe dry powder, fermented dry yeast, grain germ enzyme, and trace amounts of seaweed. A number of fish feeds to which various ingredients are applied to prevent diseases of fish such as fish have been proposed.

As such, in the case of fish feed, it is also a problem that it is vulnerable to fish diseases, but in recent years, as the economy develops rapidly and the standard of living improves, the demand for high-quality animal protein is increasing. Due to the lack of feed resources, it has a considerable impact on the development of the livestock industry and the ornamental fish industry. For this reason, in many countries, artificial breeding of insects is used as a major strategy to solve protein feed sources, and among them, the development of feeds applied to Dongae, etc. is one example.

Conventional Korean Patent No. 10-1406688 has proposed a feed for ornamental fish containing mealworms and Dongae, etc., here, by producing a feed for ornamental fish containing a large amount of protein and useful ingredients, the effect of import substitution, suppression of fry mortality, and white spot disease It is described as being effective as a functional feed such as prevention, improvement of water quality in fish tanks, and reduction of excreta, as well as the effect of increasing the survival rate of fry in addition to sales.

In addition, in Korea Patent No. 10-1560791, for the cultivation of carnivorous surface fish produced by mixing larvae with microbially fermented rice bran, Dongae, etc. and larvae with Dongae, etc. in a specific ratio, and then adding oil-soluble microorganisms to the pulverized mixed pulverized product. material is proposed.

As described above, in the case of ornamental fish feed, it is important to solve all nutritional problems such as disease and protein supply. It is urgently needed to develop a feed that can quickly react to the protein, fat, and carbohydrates to be easily converted into energy. However, for this purpose, it is not easy to manufacture high-quality feed because synthesis as a catalyst is difficult in the process of composition of feed ingredients and requires advanced technology.

In addition, in the manufacturing process of ornamental fish feed, imported feed with 43% market share in the grain type and granule type market structure is granule type, and about 35% of domestic production is grain manufacturing method by pellet machine, and 22% is extrusion. It is manufactured by the grain manufacturing method by machine. Here, it is known that the manufacturing technology using the pellet machine and the extrusion machine, which are domestic manufacturing machines, has the following disadvantages.

division Contents nutrients Reduced content of nutrients and beneficial ingredients due to self-heating injury The weight of the particles is heavy and the floating is only about 60% to 70%, so there is a lot of wasted feed. pollution degree Water pollution due to sinking feed is high, so the number of times to clean the tank is frequent. mortality Increased mortality rate of ornamental fish due to bacterial production and disease outbreak due to water pollution color and luminescence Natural pigments such as astaxanthin are not as efficient (due to loss due to high temperature).

Unexamined Patent Publication No. 1998-086536 Registered Patent Publication No. 10-1406688 Registered Patent Publication No. 10-1560791

Mathews, R A. Parasitic Diseases of Fish, pp. 17-42, Tresaith, UK: Samara Publishing, 1994 Ji Bo-young et al. 1996. Characteristics of white spot, Ichthyophthirius multifiliis by developmental stage. Journal of Korean Medical Association, 9, 21 Luzardo-A''lvarez, A. et al. 2003. Oral pharmacological treatments for Ichthyophthiriosis of rainbow trout (Oncorhynchus mykiss). Aquaculture, 220, 15 Paivi R. et al. 2005. Treatment of ichthyophthiriasis after malachite green. I. Concrete tanks at salmonid farms. Dis. Aquat. Org., 64, 69 Ekanem, A. P. et al. 2004. Effects of extracts of Mucana pruriens(Fabaceae) and Carica papaya(Caricaceae) against the protozoan fish parasite Ichthyophthirius multifiliis. Parasitol. Res., 92, 361

In order to solve the problems of the prior art as described above, the present invention can prevent fish diseases such as white spot disease without using a separate antibiotic as well as increasing the content of crude protein and crude fat in consideration of digestion and absorption and nutrition in composing feed for ornamental fish. The task to be solved is to show the effective effect.

The present invention breaks the conventional wisdom of cultured ornamental fish through complex administration of fishmeal feed and antibiotics, or cultured ornamental fish using imported feed to which various chemicals are added. It provides an ornamental fish feed of the concept.

Accordingly, it is an object of the present invention to provide a high-quality ornamental fish feed having an effect of treating and preventing white spot disease and having excellent digestion and absorption and nutrient content.

Another object of the present invention is to provide an ornamental fish feed in which omega nuts are mixed in a specific ratio in order to use dongae, etc. as a substitute for fish meal and to obtain an effect of treating and inhibiting white spot disease.

Another object of the present invention is to provide a method for preparing an ornamental fish feed prepared by mixing Dongae and omega nuts in a specific ratio.

In order to solve the problem of the present invention as described above, it provides an ornamental fish feed composition in which Dongae and omega nuts are mixed in a specific ratio.

The feed composition of the present invention contains 15-25 parts by weight of powder, preferably 17-24 parts by weight, more preferably 20-22 parts by weight, based on 100 parts by weight of the total feed composition. If the powder content of Dongae, etc. is less than 15 parts by weight, the effect of increasing the crude protein and crude fat content is insignificant. found to do

In addition, there is provided an ornamental fish feed composition comprising 10-20 parts by weight of omega nuts, preferably 12-18 parts by weight, and more preferably 15-17 parts by weight, based on 100 parts by weight of the total feed composition. If the omega nut content is 10 parts by weight or less, treatment and suppression of white spot disease cannot be expected, and if it is more than 20 parts by weight, there is a problem of indigestion or reduced growth of the ornamental fish, and the tail and fins of the fish are abnormal due to excessive supply of nutrients. signs were found to occur.

As described above, when the content of a specific component is out of the above range, there is a problem in that the physical properties of the feed, such as the floating of the feed and the generation of a large amount of debris, are also greatly reduced.

Dongae used in the present invention shows a significantly superior effect compared to protein feed using other insects or worms. Dongae, etc. has recently been slightly applied to some feeds, but there has been no case of mixing it with omega nut ingredients to increase the effect of nutrients such as crude protein. According to the present invention, it was analyzed that Dongae, etc. had the characteristics shown in Table 2 below as a result of protein and amino acid content analysis.

Inspection items unit test results basis for inspection crude protein % 56.42 GB/T6432-94 moisture % 6.40 GB6435-86 Salmonella ppm not detected GB13091-91 coliform ppm not detected Animal Therapeutic Standards Taurine % not detected GB/T14965-94 HYDROXYPROLINE % not detected GB/T14965-94 ASPARTIC ACID % 5.10 GB/T14965-94 TREONINE % 2.34 GB/T14965-94 SERINE % 2.96 GB/T14965-94 GLUTAMIC ACID % 8.85 GB/T14965-94 GLYCINE % 3.50 GB/T14965-94 ALANINE % 5.08 GB/T14965-94 CYSTINE % 4.26 GB/T14965-94

In addition, Dongae, etc. contains various inorganic salts. Table 3 below is the analysis result of the inorganic salt content contained in common dongae.

K
(g/kg) Na
(g/kg) Ca
(g/kg) P
(g/kg) Mg
(g/kg) Fe
(mg/kg) Zn
(mg/kg) Cu
(mg/kg) Mn
(mg/kg) 13.7 0.656 1.38 6.83 1.94 65 0.122 25 13

On the other hand, in the present invention, omega nut, a pure natural substance that exhibits a synergistic effect by mixing with Dongae, etc. as described above, has long been known to help prevent cardiovascular diseases due to its high content of omega fatty acids.

Omega nut used in the present invention (Omega nut, Sacha Inchi) is Inca-peanut (Inca-peanut), inca-nut (Inca-nut), star seed (star-seed), mountain peanut (mountain-peanut) It is a type of nut that has been cultivated by the indigenous peoples of Peru for centuries. Currently, it is grown little by little in Southeast Asia including Thailand and parts of Korea. The fruit contains 4 to 5 seeds, and the seeds are rich in protein, fat, and essential minerals. have. Therefore, it has a very high content of omega fat and is spotlighted as a food that is helpful for cardiovascular health. The amount of unsaturated fatty acids is 3.5 times that of mackerel, 12 times that of salmon, and 2,500 times that of almonds. The content of vitamin E is 12 times that of olives and corn. 21 times more abundant.

In other words, omega nuts are fruits that contain 93% of unsaturated fatty acids omega-3, omega-6, and omega-9.

When omega-3 enters the body, it is converted into EPA (eicosapentaenoic acid) or DHA. In particular, EPA reduces triglycerides in the blood, helps white blood cells function, alleviates dermatitis such as atopic diseases, and helps prevent skin aging. give. In addition, DHA is a major component of the neural tissue of the brain and retinal tissue of the eye, and it helps to improve memory and develop brain learning ability by activating cranial nerve cells.

Omega-6 helps to reduce inflammation and reduce vasoconstriction.

Omega-9 helps to lower LDL (low density lipoprotein), which is bad cholesterol, and is rich in tryptophane, an essential amino acid that helps in weight loss. When tryptophan enters the body, it is converted to serotonin, and when serotonin is insufficient, stress and anxiety increase and induce stimulant food intake.

According to the present invention, when the omega nut exhibiting the cardiovascular disease prevention effect as described above is mixed with Dongae, etc. in a specific ratio, the feed properties are excellently improved, and the growth environment of healthy ornamental fish without disease due to the combination of natural ingredients is improved. It represents a synergistic effect that can be maximized.

According to the present invention, the omega nut provides the fact that it has an excellent effect in suppressing white spot disease, which greatly affects the mortality rate of ornamental fish. White spot disease expressed in ornamental fish is not easy to treat and suppress. Therefore, suppressing the occurrence of diseases such as white spot disease by using the omega nut together with Dongae and the like in the present invention is a very characteristic result that cannot be predicted from the fact that omega nuts are generally known to be effective in preventing cardiovascular diseases.

In the present invention, a mixture of Dongae and omega nuts can be used in combination with common sweet rice ingredients. However, according to one embodiment of the present invention, it is preferable not to mix fish meal in this whole feed composition.

According to a preferred embodiment of the present invention, 30-50 parts by weight of, for example, one or more components selected from wheat flour, starch, soybean meal, and corn, as a conventional sweetened feed in the ornamental fish feed composition, based on 100 parts by weight of the total feed composition, preferably 32-40 parts by weight. If the content of the component is 30 parts by weight or less, the gelatin may not properly absorb the component and thus granules may not be formed. this increases

According to a preferred embodiment of the present invention, the ornamental fish feed composition may contain 10-30 parts by weight, preferably 16-22 parts by weight, based on 100 parts by weight of the total feed composition of squid, shrimp, or a mixture thereof. When the content is 10 parts by weight or less, the animal protein component is lower than that of insect protein and the odor of the feed is reduced, so the fish colony phenomenon and the taste of the feed are significantly reduced. can

According to a preferred embodiment of the present invention, at least one component selected from spirulina, artemia, sesame cake, mealworm, chitosan and vitamins in the ornamental fish feed composition is 3-10 parts by weight based on 100 parts by weight of the total feed composition. It may contain parts by weight, preferably 4-8 parts by weight, more preferably 5-6 parts by weight. If the content of the component is less than 3 parts by weight, the immunity and motility of the fish are lowered, and if it is more than 10 parts by weight, it causes an increase in the cost of the product.

The spirulina component is a vegetable microalgae and has effects such as improving immune function, protecting skin, and discharging waste products.

The Artemia component is zooplankton, which is mainly used as feed for fry in aquaculture, and has a protein content of 52% or more.

According to a preferred embodiment of the present invention, gelatin as a natural binder may additionally contain 1-5 parts by weight, preferably 2-4 parts by weight, more preferably 3 parts by weight, based on 100 parts by weight of the total feed composition. When the amount of the gelatin component is 1 part by weight or less, the gelatin does not absorb other components properly, so it may be difficult to form granules.

According to a preferred embodiment of the present invention, it is characterized in that no fish meal is included in the total composition of the ornamental fish feed.

According to a preferred embodiment of the present invention, 1-5 parts by weight of astaxanthin, preferably 1-3 parts by weight, may be additionally contained in the ornamental fish feed composition. When the astaxanthin content is 1 part by weight or less, the function as a natural colorant is insignificant and the red color does not appear.

The present invention provides a method for preparing an ornamental fish feed composition by combining Dongae and omega nuts.

The present invention comprises the steps of mixing 15-25 parts by weight of powder and 10-20 parts by weight of omega nuts in Dongae, etc. with respect to 100 parts by weight of the total feed composition; aging at low temperature; A step of mixing a conventional simple feed here; and grinding the mixture; It provides a method for producing a fish feed composition comprising a; granulating using a fluidized bed granulator.

According to a preferred embodiment of the present invention, in the step of mixing the conventional simple feed, one or more components selected from wheat flour, starch, soybean meal and corn are added in an amount of 30-50 parts by weight based on 100 parts by weight of the total feed composition, squid, shrimp or these 10-30 parts by weight of the mixed component, Spirulina, Artemia, Sesame cake, mealworm, chitosan, and may include mixing 3-10 parts by weight of one or more components selected from vitamins.

According to a preferred embodiment of the present invention, the conventional simple feed is characterized in that it does not contain fishmeal.

According to a preferred embodiment of the present invention, the ornamental fish feed composition of the present invention is first mixed with 15-25 parts by weight of omega nut powder and 10-20 parts by weight of dongae, etc. with respect to 100 parts by weight of the total feed composition, and then secondarily It is preferable to prepare by a method of mixing a conventional simple feed.

According to a preferred embodiment of the present invention, after the first mixing, a low-temperature aging step is performed. In this case, the low-temperature aging is preferably performed at 0-10° C., preferably at 3-7° C., more preferably at 5° C., followed by mixing with conventional sweetened feed. Outside the range of the low temperature aging temperature may affect the moisture content and quality of the feed.

In the present invention, it is preferable to granulate the mixture without going through a drying process after pulverizing it to prepare a feed.

According to the present invention, granulation is preferably performed using a fluidized bed granulator. The granulated form of the ornamental fish feed prepared according to the present invention may be prepared in the form of granules as shown in the photograph in FIG. 1 .

According to the present invention, when the feed is manufactured by a conventional pellet machine or extrusion machine without using a fluidized bed granulator, various disadvantages as shown in Table 1 above appear, which may impede the expression of the desired effect of the present invention. can

According to the embodiment of the present invention, the granular fish feed prepared by the fluidized bed granulator as described above has a form that can be digested and absorbed very well in water, so disease prevention and healthy and excellent developmental status in the growth process of the ornamental fish can be achieved. This helps to significantly reduce the mortality rate.

As an existing product, the maximum value of crude protein due to the combination of fishmeal and other sweetened feed was 35%. The maximum value of crude protein could be obtained up to 54% or more, and at least the crude protein content was much higher than 42%, so it is superior even when compared to imported feed.

Therefore, it was confirmed that the ornamental fish feed of the present invention exhibits an effect of increasing crude protein by 7-19% by weight or more compared to a feed using conventional fish meal, and significantly inhibits the occurrence of fish diseases such as white spot disease. This is thought to be due to the fact that in the present invention, Dongae and omega nuts were mixed in a specific ratio and granulated with a fluid bed granulator without drying process.

As such, the present invention is a feed for ornamental fish, which is a mixture of dongae and omega nuts as a substitute for fishmeal, by adjusting the mixing ratio of each sweet feed to a specific composition, thereby increasing the crude protein and crude fat content, as well as preventing and treating white spot disease. feed can be manufactured.

In recent years, most imported feeds are in the form of granular particles, highlighting that they are flotation and residue-free feeds, leading to consumer response.

However, the present invention is light in weight compared to fish meal feed, contains more than 48% of crude protein, and has the best flotation property due to the occurrence of bubbles in the particles. In addition to improvement, it was possible to treat and suppress white spot disease, a chronic disease in Korea. Therefore, the present invention contributes to the saving of foreign currency due to the import substitution effect and enables the use of environmentally friendly feed by using natural ingredients, which is economically and environmentally very effective.

The ornamental fish feed containing dongae and omega nuts according to the present invention has an effect of preventing the occurrence of fish diseases without the use of separate pharmaceuticals as an effect of preventing diseases such as white spot disease.

The present invention exhibits remarkably excellent effects not only in increasing crude protein and preventing fish diseases, but also in various feed characteristics such as flotation of feed, water pollution, color and luminescence characteristics, survival rate of fry, digestion and absorption, etc. .

In addition, instead of antibiotics or preservatives in general feed, dongae and omega nut are formulated in a specific ratio to significantly reduce the mortality rate of ornamental fish, and the crude protein content can be increased, thereby increasing the growth rate through efficient supply of nutrition. It can greatly contribute to the income increase of omega nut producers.

In addition, it is possible to simultaneously improve price competitiveness and quality competitiveness through the immune-enhancing effect while using feed using natural substances, and it is possible to protect fish species as well as significantly lower the loss rate of the aquaculture industry. this is expected

In addition, the use of natural ornamental fish feed has the effect of reducing costs and enabling export due to the substitution effect for imported feed.

1 is a photograph showing the granular product form of an ornamental fish feed including dongae and omega nuts manufactured according to the present invention.

Hereinafter, the contents of the present invention will be described in detail through Examples and Experimental Examples. However, these are only for describing the present invention in more detail, and the scope of the present invention is not limited thereto.

<Production Example>

According to the method shown in Table 4 below, a compound feed for ornamental fish mixed with dongae and omega nuts was prepared, and for comparison with this, a feed for ornamental fish using conventional fish meal was prepared.

work detail Manufacturing process and differences note In the case of feed using fish meal In the case of Dongae and omega nut compound feed Grinder usage time 150kg/65min 150kg/23min Reduced electricity bills and working hours dust generation Until the end of production time doesn't exist work environment improvement fishmeal smell large amount doesn't exist work environment improvement Dryer (curing) 150kg/50min not needed Reduce electricity bills rancidity 8% 0.5% Cost Reduction feed particle shape Grain Granual Expectation of substitution effect of imported goods levitation 80% 98% or more Same as imported Nutrient destruction rate 30% less than 5% Competitive with imported goods automation rate 60% 100% Producer Labor Reduction Loss by sorter 23% less than 5% Cost Reduction Mixer cleaning time 10 sessions/month 8 sessions/month Producer Labor Reduction manufacturing machine pellet fluid bed granulator Reduced producer working time and reduced fatigue due to automation Pellet machine cylinder replacement cycle replacement after 6 months Not applicable Possible to improve the factory environment Grinder (50HP) Belt replacement 4 times/month Not applicable Reduce Consumables dust collector cleaning 4 times/month 2 times/month Producer Labor Reduction grease injection 2 times/month Not applicable Reduce producer fatigue electricity bill 680,000/1 month 430,000 won/month 36.8% reduction

By applying an actual ornamental fish feed to each of the prepared feeds, the results were comparatively evaluated and shown in Table 5 below.

division fish meal feed Dongae Dungae and Omega Nut Mixed Feed temperature and foaming The composition particles formed at a temperature of 80° C. have a foamability of about 63% and a low foaming efficiency, resulting in less flotation. The temperature inside the cylinder is 43℃, and granular particles are easily formed due to up-and-down movement and friction by high-pressure air injection. feed weight The high particle density makes the composition heavy. It is easy to foam particles, so the weight is light and the flotation efficiency is improved. moisture content Due to the high moisture content of the particles discharged from the pellet machine, the power consumption increases, such as a longer drying time. There is no need for a separate curing time, and the moisture meter test result is less than 5%. natural colorant The efficiency of astaxanthin for color development and light emission is low. There is no loss of natural colorants due to good mechanical specifications and coefficient of expansion. particle shape Nutrient loss due to autogenous heat is great. The particle shape is a grain type, and it is inferior in competitiveness with the granular type of imported feed. As a result of component analysis, the amount of loss was greatly reduced and technological innovation was achieved by manufacturing perfect granular particles.

From the above results, it was confirmed that the feed in which Dongae, etc. and omega nut was blended had a very good effect compared to the fish meal feed in temperature and foaming properties, feed weight and flotation properties, moisture content, degree of color loss, and particle shape.

<Examples 1 to 10> Preparation of ornamental fish feed composition

An ornamental fish feed mixed with Dongae backae and omega nut was prepared in various proportions as shown in Table 6 below. In Table 6, the unit is parts by weight.

Example
Raw material name One 2 3 4 5 6 7 8 9 10 final
Selection of mixing ratio in fraternity 20.5 22 24 22 18 22 22 17 22 21.5 22 Omega Nuts 15 18 20 17 12 16 16 15 18 18 17 wheat flour 10 9 9 7 10 8 9 10 9 9 9 starch 10 10 9 8 10 8 9 10 9 9 9 Soybean Meal 8 7 7 10 10 9 9 10 9 9 8.5 corn 8 7 7 8 10 9 9 10 9 9 8.5 squid powder 10 10 8 10 11 10 9 10 8 9 9 shrimp 10 8 8 10 11 10 9 10 8 8 9 spirulina 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Artemia 2 2 2 2 2 2 2 2 2 2 2 sesame cake One One One One One One One One One One One Mealworm 0.5 One One One One One One One One 0.5 One vitamin A 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 gelatin 3 3 2 2 2 2 2 2 2 2 2 chitosan 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 astaxanthin 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 total 100 100 100 100 100 100 100 100 100 100 100

The results of analyzing the ingredients of the feed according to the final blending ratio are shown in Table 7 below. In Table 7 below, the unit is % by weight.

ingredient
Example crude protein
(% More than) george
(% More than) calcium
(% More than) views
(% below) sign
(% below) crude fiber
(% below) moisture
(% below) 2020.9.11 53.8 10.11 2.3 13.28 1.94 2.95 8.76

The results of testing the harmful substances in the feed according to the final mixing ratio are shown in Table 8 below.

division 2020.9.11. request Test method arsenic non-detection Feed standard analysis method fluoride 95.31 same as above chrome non-detection same as above lead non-detection same as above Mercury 0.01 same as above cadmium non-detection same as above selenium (Se) 0.25 same as above Aflatoxin B1 non-detection same as above Okratoxin A non-detection same as above Aflatoxin B2 non-detection same as above Aflatoxin G1 non-detection same as above Volatile basic nitrogen 0.04 same as above Silky white nitrogen 0.04 same as above Salmonella D group non-detection same as above

<Experimental Example 1> Comparison of components of Dongae and other insects

Although the content of beneficial ingredients is not necessarily advantageous compared to other insects through the analysis of ingredients for Dongae, etc., when mixed with omega nut, very favorable results were expressed due to synergistic effect. Table 9 (component content), Table It could be confirmed by the component analysis table of 10 (inorganic salt content) and Table 11 (amino acid content).

insect name moisture
(g/kg) skim
(g/kg) protein
(g/kg) carbohydrate
(mg/kg) Ammonium Sulfate
(mg/kg) vitamin B2
(mg/kg) vitamin E
(mg/kg) in fraternity 34 405.0 384 96 0.06 5.8 4.9 Mealworm 37 388.0 489 107 0.65 5.2 4.4 wild silkworm 45 280.0 570 85 0.50 62.0 35.0 silkworm pupa 40 71.9 714 109 - - - Grasshopper 31 76.5 705 128 - - - bee pupa 38 264.0 353 - - - - ant 41 192.0 695 - - - -

insect name k
(g/kg) Na
(g/kg) Ca
(g/kg) P
(g/kg) Mg
(mg/kg) Fe
(mg/kg) Zh
(mg/kg) Cu
(mg/kg) Mn
(mg/kg) Se
(mg/kg) in fraternity 14.20 0.632 1.25 6.91 1.850 64 0.119 43 15 0.475 Mealworm 13.7 0.656 1.38 6.83 1.940 65 0.122 25 13 0.462 bee pupa - 8.600 4.80 1.95 0.016 191 0.064 21 350 0.175 silkworm pupa 11.35 0.311 9.50 6.05 3.100 170 0.014 2 One 0.175 cicada 3.00 - 0.17 5.80 - - 0.82 - - -

insect name Tryptophan Threonine Methion
ine+Cystine Isoleucine PhenyLanine+tyrosine Lysine Valine Leuchine in fraternity 1.0 4.83 2.60 0.82 8.97 6.24 9.00 10.51 Mealworm 1.0 5.00 2.62 5.85 9.07 6.27 9.06 9.36 pupa 1.0 5.00 0.38 7.46 6.9 7.25 6.17 7.04 infant
required ratio 1.0 5.10 3.40 9.50 1.10 7.40 6.00 5.50 adult
required ratio 1.0 2.00 3.70 4.00 2.90 4.00 3.40 2.90

In Table 11, the unit is (mg/kg).

<Experimental Example 2> Investigation of white spot inhibitory effect

In order to confirm the inhibitory effect of white spot disease on the ornamental fish feed according to Example 1, an experiment was conducted as follows.

(1) blood characteristics

For blood collection, 6 numbers are randomly selected for each treatment, and at the end of the test, 2ml of blood is collected from the heart using K3EDTA Vacuum tube (Becton Dickinson Vacutainer System. ) was analyzed.

(2) Investigation of the inhibitory effect of white spot disease

It is a disease caused by parasitization of the ciliates Ichthyophthyrius Multifilis (freshwater fish) and Cryptocaryonirritans (saltwater fish) on the epidermis and gills. The change of white spot disease according to the omega nut feed was measured using Real Time PCR. After collecting tissues for each treatment group, DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega, USA) and stored at 70°C. For Real Time PCR reaction solution, 10ng of Template Genomic DNA, 10μl of Quantispeed Probe No-Rox Kit, 0.8μl of Forward and Reverse Primer, and 0.4μl of Probe were added, and sterilized tertiary distilled water was added so that the final volume was 20μl, Chromo4 Detector (Bio-Rad, UAS) was reacted.

(3) Immune antibody investigation in blood

2ml of blood was collected from the pterygoid vein, transferred to a vacutainer, left at room temperature for 2 hours, centrifuged at 2,500 rpm for 10 minutes, and serum was separated and stored at 20°C. Analysis of serum Ig The concentrations of IgA, IgG and IgM were investigated by enzyme immunization. In order to obtain the antioxidant level in blood, after the end of the experiment, two individuals maintaining a similar body weight were selected for each treatment repetition, and blood was collected from the heart. 0.2 ml of plasma was separated from the collected blood, added to a test tube containing 3 ml of pH85 Tris-HCI solution, and then 0.1 ml of 3 mM Pyrogallol was added and incubated at 25° C. for 30 minutes. After incubation, 0.1 ml of 1N HCl was added to stop the reaction, and absorbance was measured at 420 nm (At). Also, in the same way, the absorbance was measured using a UV/VIS Spectrometer (Perkin Elmer, USA) at 40 nm for the treatment group in which only plasma was added (Ac), and in the treatment group in which only plasma was added to Tris-HCI solution and Pyrogallol was not added (Ao). was measured and the degree of antioxidant was obtained by Equation 1 below.

[Equation 1]

Antioxidation degree (%) = [1-(Ac-At)/Ao] × 100

The results obtained in this test were performed using the GLM Procedure of the SAS Package Program, and the significance (P<0.05) of the treatment section was verified by the Ducan multiple test.

Based on this, the process of suppressing white spot disease in feed is shown in Table 12 and Table 13 below. Here, W is the input quantity of ornamental fish with white spot disease, and D is the number of dead ornamental fish.

division ornamental fish Early
(20.4.8) 4/23 5/12 5/27 6/11 6/23 7/8 W D W D W D W D W D W D W D freshwater fish Red and White Nishikigoi 25 One goldfish 49 2 2 One 5 3 2 tropical fish
(guppies) wild goofy 18 One One yellow tuxedo 21 2 One tropical fish
(Caracine) neon tetra 34 One One One cardinal tetra 13 One One tropical fish
(Secrets) angel fish 27 2 One One total 187 0 0 7 0 6 0 2 2 5 4 4

ornamental fish Early
(20.4.8) 7/27 8/10 8/26 9/11 9/28 10/13 10/26 survival rate
(number of animals) W D W D W D W D W D W D W D W D Red and White Nishikigoi 25 96% (24) goldfish 49 2 One 2 One 5 One One 63% (31) wild goofy 18 One 83% (15) yellow tuxedo 21 One 81% (17) neon tetra 34 One One One 82% (28) cardinal tetra 13 One One 69% (9) angel fish 27 One 81% (22) 187 0 0 3 0 2 0 3 0 2 5 2 0 2 0 2

From the above results, it was confirmed that the state of the tank was abnormal when the feed according to the example was added, and thus had excellent environmental characteristics for breeding ornamental fish, and the prevention and suppression effect of ornamental fish infected with white spot disease was 68.8% or more on average. In addition, it can be expected that some people have a therapeutic effect on white spot disease.

The effect of preventing white spot disease is at a level that is not significantly different from the case of using a separate treatment when using the existing fish meal feed. Therefore, considering that it is a feed using natural ingredients, it can be confirmed that the effect is very good.

<Experimental Example 3> Water tank administration experiment of feed

For the ornamental fish feed prepared in the above example, the state of the tank was observed through the administration experiment of the tank as shown in the following Table 14 in the same manner as in the experimental example. Here, the size and quantity of the water tank is 685 x 485 x 415mm (10 units installed), and the result is as follows.

division ornamental fish
type size
(cm) Experiment
Marisu feed size
(mm) 1 day
feed administration
number 1 company feed dose
(g) 1 day LED irradiation
hour Oxygen and filter types Average
temperature
(℃) freshwater fish Red and White Nishikigoi 22.5 11 0.6 3 3 4 side 20 25.3 14 0.6 3 3 4 side 20 goldfish 9 26 0.4 3 3 5 side 20 12.3 23 0.4 3 3 5 side 20 tropical fish
(guppies) wild goofy 5.1 18 0.1 3 2 6 side 22 yellow tuxedo 4.5 21 0.1 3 2 6 side 23 tropical fish
(Caracine) neon tetra 4.2 34 0.1 3 2 6 side 22 cardinal tetra 4.3 13 0.1 3 2 6 side 22 tropical fish
(Secrets) angel fish 4.2 27 0.1 3 2 6 side 22 pencil fish 4.9 29 0.1 3 2 6 side 22 total 8 kinds 216

There were 8 types of experimental ornamental fish, a total of 216, and the results were obtained through research and experiments over 8 months.

Table 15 below shows the results of examining the operating status of the tank components after administration of the ornamental fish feed.

division 2020.4. 5 6 7 8 9 10 11 Oxygen generator (twin) O O O O O O O O side filter O O O O O O substitute O heater O O O O O O O O LED O O O O substitute O O O automatic feeding machine O O O O O O O O filter sponge cleaning 1 time 1 time O 1 time 1 time O 1 time 1 time feed pump O O O O O O O O automatic electricity supply controller O O O O O O O O number of water changes 1 time 1 time 1 time 1 time 1 time 1 time O 1 time

Here, a good operating condition is denoted by O, and usually denoted by Δ.

<Experimental Example 4> Analysis of color development and luminescence effect of ornamental fish

For the ornamental fish feed prepared in the above example, the color development and luminescence effects were analyzed in the same manner as in the experimental example, and the growth state of the ornamental fish was observed.

In the monthly color test according to the type of ornamental fish, a Japanese Konica Minolta (CM-2600D) colorimeter was used for the test, and a colorimetric unit was used to distinguish colors. The sample result by the colorimeter was expressed as LAB, and the value was expressed in a range of 1-10.

The results of confirming the color development effect after administration of the ornamental fish feed are shown in Table 16 below.

division ornamental fish 2020.4. 5 6 7 8 9 10 11 freshwater fish red and white
Nishikigoi 5 6 6 7 7 8 8 8 goldfish 5 5 6 6 6 7 7 8 tropical fish
(guppies) Mosaic
tuxedo 4 5 5 6 6 6 7 7 wild goofy 4 5 5 6 6 7 7 7 yellow tuxedo 4 5 6 6 6 7 7 7 tropical fish
(Caracine) neon tetra 4 4 5 5 6 6 7 7 cardinal tetra 4 5 5 6 6 7 7 8 tropical fish
(Secrets) angel fish 4 4 5 5 6 6 7 7 pencil fish 4 5 5 6 6 7 8 8

For the monthly luminescence test according to the type of ornamental fish, a spectrophotometer from Kyungin Science Co., Ltd. (KSIC-3192) was used, and among them, a prism spectrometer was used. The luminescent effect after administration of the ornamental fish feed is shown in Table 17 below. Here, good is indicated by ○, and normal is indicated by △.

division ornamental fish 2020.4. 5 6 7 8 9 10 11 freshwater fish red and white
Nishikigoi △ ○ ○ ○ ○ ○ ○ ○ goldfish △ ○ ○ ○ ○ △ ○ ○ tropical fish
(guppies) Mosaic
tuxedo △ △ ○ ○ △ ○ ○ ○ wild goofy △ △ △ ○ ○ ○ ○ ○ yellow tuxedo △ ○ ○ ○ ○ ○ ○ ○ tropical fish
(Caracine) neon tetra △ △ ○ ○ ○ ○ ○ ○ cardinal tetra △ △ ○ ○ ○ ○ ○ ○ tropical fish
(Secrets) angel fish △ △ ○ ○ ○ ○ ○ ○ pencil fish △ △ ○ ○ ○ ○ ○ ○

From the results of the table above, when the ornamental fish feed of the present invention was put into the tank, it was found that the color and luminescence of the ornamental fish was abnormal, indicating that a healthy growth state was possible, and the beautiful appearance of the ornamental fish was continuously displayed. It was found that one ornamental fish feed was effective for color development and luminescence.

<Experimental Example 5> Physical performance test of water tank after feed administration

The results of confirming the physical performance of the aquarium fish feed prepared in the above Example in the tank to which the feed was applied in the same manner as in the Experimental Example are shown in Table 18 below.

division 2020.4. 5 6 7 8 9 10 11 levitation 94% 95% 96% 96% 96% 95% 96% 95% sinking 5% 4% 5% 3% 3% 3% 4% 4% resurfacing 5% 5% 8% 7% 8% 8% 8% 8% 流速 (drifting) Good Good Good Pump
substitute Good Good Good Good Decomposition rate Feed diameter: 0.5cm 9 minutes 8 minutes 8 minutes 7 minutes 8 minutes 9 minutes 8 minutes 7 minutes Decomposition rate Feed diameter: 0.2cm 4 minutes 5 minutes 4 minutes 4 minutes 4 minutes 5 minutes 4 minutes 4 minutes 群落 (cold) Good Good Good Good Good Good Good Good Remaining amount (remnants) 0.5% 0.5% doesn't exist doesn't exist doesn't exist doesn't exist doesn't exist doesn't exist fecal condition Good Good Good usually Good Good Good Good automatic pay system Good Good 2
substitute Good Good Good Good Good

As described above, the ornamental fish feed according to the present invention uses dongae and omega nuts in a specific mixing ratio, so that, compared to the existing fish meal feed, not only the effect of increasing crude protein and crude fat and preventing white spot disease, but also various feed characteristics. It is confirmed that this is a synergistic effect due to the mixed use of Dongae and omega nuts.

The main features appearing in the feed for ornamental fish of the present invention can be summarized as shown in Table 19 below.

division Improved properties and properties of the present invention ingredient amount Increased crude protein and crude fat ornamental fish disease Prevention and suppression of white spot disease levitation 18% improvement compared to using fishmeal Water Pollution Degree 21% reduction compared to using fishmeal generation and luminescence 9% increase compared to using fishmeal fry survival rate 16% increase compared to using fishmeal Number of times of tank cleaning Reduced from 2-3 times a month to once a month particle shape Converted from pellet machine-molded particle grain type to fluid bed granulator and converted to granule type (improving flotation and providing feed free of debris)

<Experimental Example 6> Measurement of rancidity of feed

The degree of rancidity was measured for the ornamental fish feed prepared in the above Example. As a result of measuring the rancidity of the feed 90 days after feed production, there was no change in the control group and all treatment groups. To measure the rancidity of the feed 90 days after production, 4 g of the analyte is mixed with 10 ml of a 20% trichloroacetic acid solution, 10 ml of distilled water (DW) is added to the mixture, and homogenized by shaking. The mixture was filtered with Whatman No. 1 filter paper, 2 ml of the filtrate was transferred to a test tube, and 2 ml of 2-thiobarbituric acid (0.005 M in DW) was added. After installing the tube, the mixture is mixed by conduction and heat-treated in a constant temperature water bath at 90°C for 30 minutes. It was multiplied by 5.88 and expressed as MDA umol/100g (Vernon et al., 1970).

The results of the rancidity test of the feed 90 days after production are shown in Table 20 below.

division Average standard error p-value control 2.987 +/- 0.0582 0.856 treatment area 1 2.910 +/- 0.0893 treatment area 2 2.969 +/- 0.0687 treatment area 3 2.963 +/- 0.0388

The degree of rancidity was measured according to the temperature and humidity of the livestock farm, and the results are shown in Table 21 below.

rancidity test cattle farm temperature 23℃ 23℃ 23℃ 23℃ 23℃ 23℃ 22℃ 23℃ Farm Humidity 50% 47% 45% 48% 50% 53% 48% 48% condition of feed good good good good good good good good

<Comparative Examples 1 to 4>

An ornamental fish feed was prepared by changing the content of each composition as shown in Table 22 below.

comparative example
Raw material name One 2 3 4 in fraternity 30 12 27 10 Omega Nuts 7 30 23 7 wheat flour 10 9 7 10 starch 10 10 7 10 Soybean Meal 8 7 6 15 corn 8 7 6 11 squid powder 10 10 8 15 shrimp 10 8 9 15 spirulina 0.5 0.5 0.5 0.5 Artemia 2 2 2 2 sesame cake One One One One mineral One One One One vitamin A 0.5 0.5 0.5 0.5 Chaejongbak One One One One Sodium Glutamate 0.5 0.5 0.5 0.5 astaxanthin 0.5 0.5 0.5 0.5 total 100 100 100 100

The component analysis results of the prepared feed are shown in Table 23 below. Looking at the results, it shows a significantly lower result than in the above example. In the table below, the unit is % by weight.

ingredient
comparative example crude protein
(% More than) george
(% More than) calcium
(% More than) views
(% below) sign
(% below) crude fiber
(% below) moisture
(% below) One 40.7 3.9 0.7 14.2 3.4 5.6 10.1 2 28.5 5.6 0.5 15.7 2.8 4.7 11.3 3 36.7 4.2 0.6 15.3 2.7 5.2 12.7 4 25.4 2.3 0.3 15.1 2.1 5.1 10.5

<Comparative Example 5>

The components of the feed of the present invention prepared under the same conditions of the above example and the conventional fish meal feed were analyzed and compared. The results are shown in Table 24 below.

division fish meal feed Dongae Dungae and Omega Nut Mixed Feed rate of change Crude protein (% or more) 38.0% 54.0% +16% Crude Fat (% or more) 4.8% 10.11% +5.31% Calcium (more than %) 1.0% 1.38% +0.38% Crude Fiber (% or less) 6.5% 2.95% -3.55% Views (% or less) 16.9% 13.28% -3.62% Phosphorus (% or less) 3.5% 1.94% -1.56% Moisture (% or less) 12.1% 8.76% -3.34%

Claims (8)

An ornamental fish feed composition containing 15-25 parts by weight of powder and 10-20 parts by weight of omega nut relative to 100 parts by weight of the total feed composition. The method according to claim 1, wherein, based on 100 parts by weight of the total feed composition, 30-50 parts by weight of at least one component selected from wheat flour, starch, soybean meal and corn, 10-30 parts by weight of squid, shrimp or a mixture thereof, spirulina, artemia, An ornamental fish feed composition, characterized in that it additionally contains 3-10 parts by weight of at least one component selected from sesame cake, mealworms, chitosan and vitamins. The ornamental fish feed composition according to claim 1, wherein fishmeal is not included in the total composition. The ornamental fish feed composition according to claim 1, further comprising 1-5 parts by weight of gelatin based on 100 parts by weight of the total feed composition. The ornamental fish feed composition according to claim 1, characterized in that it additionally contains 1-5 parts by weight of astaxanthin relative to 100 parts by weight of the total feed composition. Mixing 15-25 parts by weight of powder and 10-20 parts by weight of omega nuts in Dongae, etc. based on 100 parts by weight of the total feed composition; aging at low temperature; Mixing a conventional simple feed here; and grinding the mixture; A method for producing a fish feed composition comprising a; granulating using a fluidized bed granulator. [Claim 7] The method of claim 6, wherein in the step of mixing the conventional simple feed, 30-50 parts by weight of at least one component selected from wheat flour, starch, soybean meal, and corn based on 100 parts by weight of the total feed composition, squid, shrimp, or a mixture thereof 10-30 parts by weight, spirulina, artemia, sesame cake, mealworm, chitosan, and a method for producing an ornamental fish feed composition comprising the step of mixing 3-10 parts by weight of one or more components selected from chitosan and vitamins. [Claim 7] The method of claim 6, wherein the conventional sweet rice feed does not contain fish meal.

Images