KR20220125511A - Nanorization-treated fermented Hibiscus manihot with enhanced collagen, amino acid, polyphenol or flavonoid content and manufacturing method thereof - Google Patents

Abstract

The present invention relates to nano-processed fermented Abelmoschus manihot products with an increased content of collagen, amino acids, polyphenols or flavonoids and a manufacturing method thereof. By nano-processing the Abelmoschus manihot products produced by using Bacillus subtilis, it was confirmed that antioxidant activity, anti-inflammatory activity and skin wrinkle reducing or skin whitening effects are enhanced compared to fermented Abelmoschus manihot products which have not undergone nano-processing, and thus the nano-processed fermented Abelmoschus manihot products of the present invention can be usefully used in related industries.

Description

Nano-treated fermented Geumhwa beef with enhanced collagen, amino acid, polyphenol or flavonoid content and manufacturing method thereof

The present invention relates to a nano-treated fermented Geumhwasil with enhanced collagen, amino acid, polyphenol or flavonoid content, and a method for preparing the same.

Recently, the craze for inner beauty is blowing around the world. Inner beauty, through food or edible cosmetics for inner beauty, does not temporarily improve the outer surface of the skin like conventional cosmetics, but improves lifestyle and eating habits to solve fundamental health and skin problems. means to do Representative products include products containing hyaluronic acid (a component that maintains moisture in the skin), collagen (a component effective for skin elasticity and anti-aging) and vitamins, and detox juice. Inner beauty is called Nutricosmetics, and as the importance of inner nutrition as opposed to outer nutrition, which receives nutrition from the outside, is emphasized, the concept of inner beauty appeared as an intermediate form between cosmetics and food. did. In August 2018, Technavio, a market expert, reported that it expects a CAGR of 5.3% for the future outlook of the global inner beauty market.

Unlike general foods, inner beauty products emphasize human safety and functionality, and the importance of major raw materials is expanding. Research on product development equivalent to functional food is needed. Currently, overseas imported raw materials are mainly used as materials for inner beauty products, so active research and development for the development of various domestic natural materials is required.

On the other hand, Geumhwagyu ( Hibiscus manihot ) is an annual plant of the mallow family, which is native to China and is cultivated in the field. In oriental medicine, flowers are used as Hwangchok-gyu-hwa, the root is Hwang-chok-gyu-geun, and the seeds are used as medicinals. It is known to treat the symptoms of inability to urinate due to pain and to be used externally for boils and burns.

On the other hand, Korean Patent No. 1857528 discloses 'a cosmetic composition for skin whitening containing Geumhwagyu extract,' and Korean Patent No. 1951156 discloses 'Method for producing a soap containing Geumhwagyu and the manufacturing method. 'Geumhwagyu Soap' is disclosed, but there is no description of 'Fermented Geumhwagyu Soap with enhanced collagen, amino acid, polyphenol or flavonoid content and manufacturing method thereof' of the present invention.

The present invention was derived from the above needs, and the present inventors confirmed that the antioxidant activity, anti-inflammatory activity, skin wrinkle improvement, or skin whitening effect of fermented gold ginseng was enhanced through the nano-processing using fine beads. , the present invention was completed.

In order to solve the above problems, the present invention provides a health functional food composition for antioxidant, anti-inflammatory, skin wrinkle improvement or skin whitening containing nano-treated fermented Geumhwagyu as an active ingredient.

In addition, the present invention provides a cosmetic composition for anti-oxidation, anti-inflammatory, skin wrinkle improvement or skin whitening containing nano-treated fermented Geumhwagyu as an active ingredient.

In addition, the present invention comprises the steps of (a) mixing and sterilizing water, Keumhwagyu flower powder and glucose; (b) inoculating and fermenting the Bacillus subtilis strain culture solution in the sterilized solution of Keumhwagyu flowers of step (a); And (c) nano-processing the fermented product of step (b); provides a method for producing a nano-treated fermented gold fermented product containing antioxidant activity, anti-inflammatory activity, skin wrinkle improvement and skin whitening effect.

The present invention relates to a nano-treated fermented gold flavonoid with enhanced collagen, amino acid, polyphenol or flavonoid content and a method for preparing the same. Since it was confirmed that the activity, anti-inflammatory activity, skin wrinkle improvement, or skin whitening effect is improved compared to the fermented Geumhwagyu not treated with nano, it can be usefully used in related industrial fields.

1 is a result of measuring the protein content of the nano-treated fermented Geumhwagyu of the present invention. *** indicates that the protein content of the fermented fermented fermented fermented fermented fermented komhwa compared to the fermented fermented gold fermented with nano-treated silica was statistically significantly increased, and *** is p<0.001.
2 is a result of measuring the amino acid content of the nano-treated fermented Geumhwagyu according to the present invention, and is expressed as a percentage (%) compared to the distilled water treatment group. *** means that the amino acid content of the nano-treated fermented Kumhwa silicon was increased statistically significantly compared to the fermented Kumhwa silicon that was not nano-treated, and *** is p<0.001.
3 is a result of measuring the collagen content of the nano-treated fermented Geumhwagyu of the present invention. *** indicates that the collagen content of the fermented fermented fermented fermented fermented fermented komhwa compared to the fermented fermented fermented fermented fermented fermented gold with nano-treatment was statistically significant, and *** is p<0.001.
Figure 4 is the result of measuring the DPPH free radical scavenging activity of the nano-treated fermented Kumhwa silica of the present invention, and is shown as (%) compared to the distilled water treatment group. 0.1% BHT is butylated hydroxytoluene used as a positive control. *, *** mean that the free radical scavenging activity of the fermented fermented fermented fermented fermented fermented fermented gold with 0.01% BHT or nano-treated with 0.01% BHT, compared to the fermented fermented fermented fermented fermented fermented gilts, * means p<0.05, * ** is p<0.001.
Figure 5 is the result of measuring the polyphenol (A) and flavonoid (B) content of the nano-treated fermented Geumhwa silica of the present invention, and is shown as (%) compared to the distilled water treatment group. ** and *** mean that the polyphenol or flavonoid content of the fermented fermented fermented fermented fermented komhwa compared to the fermented fermented fermented Kumhwa silica was statistically significant, and ** is p<0.01, ** * is p<0.001.
6 is a result of measuring the lipoxygenase inhibitory activity of the nano-treated fermented Geumhwagyu silicate of the present invention, and is shown as (%) compared to the distilled water treatment group. 0.1% IDM is indomethacin used as a positive control. *** means that the lipoxygenase inhibitory activity of the fermented fermented fermented fermented fermented fermented fermented fermented fermented komhwag with 0.1% IDM or nano-treated Kumhwa silica was statistically significant, and *** is p<0.001. .
7 is a result of analysis of the elastase inhibitory activity of the nano-treated fermented Geumhwagyu of the present invention, and is shown as (%) compared to the distilled water treatment group. 0.1% US is ursolic acid used as a positive control. *** indicates that the elastase inhibitory activity of the fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented gold hwasil with 0.1% US or nano-treated silica was statistically significant, and *** is p<0.001. .
8 is a result of measuring the tyrosinase inhibitory activity of the nano-treated fermented Geumhwasil of the present invention, and is shown as (%) compared to the distilled water treatment group. 0.1% ABT is arbutin used as a positive control. *** indicates that the tyrosinase inhibitory activity of the fermented fermented fermented fermented fermented fermented fermented fermented fermented Kumhwa silica with 0.1% ABT or nano-treated with 0.1% ABT was statistically significant, and *** is p<0.001. .

In order to achieve the object of the present invention, the present invention provides a health functional food composition for antioxidant, anti-inflammatory, skin wrinkle improvement or skin whitening containing fermented Geumhwagyu fermented with nano-treatment as an active ingredient.

The Keumhwagyu is preferably a Keumhwagyu flower, but is not limited thereto.

In the health functional food composition for antioxidant, anti-inflammatory, skin wrinkle improvement or skin whitening according to an embodiment of the present invention, the nano-treated fermented Geumhwagyu flower powder is inoculated with a Bacillus subtilis culture solution to 20 After fermentation at ~40 ° C. for 1 to 5 days, it is preferably obtained by nanoizing treatment at 30 to 50 ° C. for 1 to 5 hours using fine beads in a nano device, more preferably at 30 ° C. After fermentation for 3 days, it is obtained by nano-treatment for 2 hours or 4 hours at 40° C. using fine beads in a nano-device, but is not limited thereto.

As used herein, the term 'nanoization' refers to pulverizing particles in a fermented product to a nano size, and preferably, fine beads may be used, but is not limited thereto.

The nano-treated fermented Geumhwagyu has improved protein content, amino acid content, collagen content, polyphenol content, flavonoid content, antioxidant activity, anti-inflammatory activity, skin wrinkle improvement effect, or skin whitening effect compared to fermented fermented Geumhwagyu beef that is not nano-treated. became

The health functional food composition of the present invention may be prepared in any one formulation selected from powder, granule, pill, tablet, capsule, candy, syrup and beverage, but is not limited thereto.

When the health functional food composition of the present invention is used as a food additive, the health functional food composition may be added as it is or used together with other foods or food ingredients, and may be appropriately used according to a conventional method. The active ingredient may be used appropriately depending on the purpose of its use (prevention or improvement). In general, in the production of food or beverage, the health functional food composition of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less, based on the raw material. However, in the case of long-term intake for health purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount greater than the above range.

There is no particular limitation on the type of the health functional food. Examples of foods to which the health functional food composition can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, beverages, tea There are drinks, alcoholic beverages, vitamin complexes, etc., and includes all health foods in the ordinary sense.

In addition, the health functional food composition of the present invention may be prepared as a food, particularly a functional food. The functional food of the present invention may include ingredients that are commonly added. Examples include proteins, carbohydrates, fats, nutrients and seasonings. For example, when manufactured as a drink, natural carbohydrates or flavoring agents may be included as additional ingredients in addition to the active ingredient. The natural carbohydrates include monosaccharides (eg, glucose, fructose, etc.), disaccharides (eg, maltose, sucrose, etc.), oligosaccharides, polysaccharides (eg, dextrin, cyclodextrin, etc.) or sugar alcohols (eg, , xylitol, sorbitol, erythritol, etc.). As the flavoring agent, natural flavoring agents (eg, taumatine, stevia extract, etc.) and synthetic flavoring agents (eg, saccharin, aspartame, etc.) may be used.

In addition to the health functional food composition, various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonic acid It may further contain a carbonation agent and the like used in beverages. The ratio of these added ingredients is not very important, but is generally selected in the range of 0.01 to 0.1 parts by weight based on 100 parts by weight of the health functional food composition of the present invention.

In addition, the present invention provides a cosmetic composition for anti-oxidation, anti-inflammatory, skin wrinkle improvement or skin whitening containing nano-treated fermented Geumhwagyu as an active ingredient.

In the cosmetic composition according to an embodiment of the present invention, the cosmetic composition is a skin, skin softener, skin toner, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, eye cream, moisture cream, hand It may have any one formulation selected from cream, essence, nutritional essence, pack, cleansing foam, cleansing water, cleansing cream, body lotion, body cleanser, soap, and powder, but is not limited thereto. The cosmetic composition consisting of each of these formulations may contain various bases and additives necessary and appropriate for the formulation of the formulation, and the types and amounts of these components can be easily selected by those skilled in the art.

When the formulation of the cosmetic composition of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as a carrier component etc. may be used.

When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydro It may contain a propellant such as carbon, propane-butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene. fatty acid esters of glycol, 1,3-butylglycol oil, glycerol aliphatic esters, polyethylene glycol or sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester; Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracanth may be used.

When the formulation of the cosmetic composition of the present invention is surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acetionate, imidazolinium derivative, methyl taurate, sarcosinate as carrier components , fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester and the like can be used.

Also, the present invention

(a) mixing and sterilizing water, Keumhwagyu flower powder and glucose;

(b) inoculating and fermenting the Bacillus subtilis strain culture solution in the sterilized solution of Keumhwagyu flowers of step (a); and

(c) nano-treating the fermented product of step (b); provides a method for producing a nano-treated fermented gold fermented product with enhanced antioxidant activity, anti-inflammatory activity, skin wrinkle improvement and skin whitening effect.

Specifically, the nano-treated fermented Geumhwagyu with enhanced antioxidant activity, anti-inflammatory activity, skin wrinkle improvement and skin whitening effect of the present invention

(a) mixing 0.5 to 1.5 parts by weight of Keumhwagyu flower powder and 0.5 to 1.5 parts by weight of glucose with respect to 100 parts by weight of water and sterilizing;

(b) inoculating the Bacillus subtilis strain culture solution in the sterilized solution of Keumhwagyu flowers of step (a) at a concentration of 5 to 15% (v/v), and fermenting at 20 to 40° C. for 1 to 5 days ; and

(c) nano-treating the fermented product of step (b) at 30-50° C. for 1 to 5 hours using fine beads in a nano-device; can be prepared including,

preferably

(a) mixing and sterilizing 1 part by weight of Keumhwagyu flower powder and 1 part by weight of glucose with respect to 100 parts by weight of water;

(b) inoculating the Bacillus subtilis strain culture solution in the sterilized solution of Keumhwagyu flowers of step (a) at a concentration of 10% (v/v), and fermenting at 30° C. for 3 days; and

(c) nano-treating the fermented product of step (b) at 40° C. for 2 hours or 4 hours using fine beads in a nano-device; may be prepared including, but not limited thereto .

Hereinafter, the present invention will be described in more detail using Preparation Examples and Examples. These preparations and examples are only for explaining the present invention in more detail, and it is obvious to those of ordinary skill in the art that the scope of the present invention is not limited thereto.

Preparation Example 1. Preparation of nano-treated fermented Kumhwa silicon

Geumhwagyu flower powder purchased from Fine Black Co., Ltd. located in Cheongju-si, Chungcheongbuk-do was used for the present invention. After adding 1 g of Geumhwagyu flower powder and 1ml of glucose to 100 ml of distilled water, it was sterilized at 121° C. for 15 minutes using an autoclave to prepare a sterilized solution for Geumhwagyu flowers. Bacillus subtilis pre-cultured for 1 day in TSB (tryptic soy broth) medium ( Bacillus subtilis , KACC 91157) strain was inoculated with 10% (v/v) in the sterilized solution for Geumhwagyu flowers, and fermented at 30° C. for 3 days to prepare a fermented Geumhwagyu. The fermented gold-silicon silicate was pulverized into particles in a Nano Max Mill (ARMSTEC) apparatus using fine beads at 40° C. for 2 hours and 4 hours to prepare a nano-treated fermented gold-silver gold fermented product.

As a comparative example, Bacillus subtilis pre-cultured for 1 day in TSB (tryptic soy broth) medium ( Bacillus subtilis , KACC 91157) strain was inoculated with 10% (v/v) of the sterilized Keumhwagyu flower, fermented at 30° C. for 3 days, and the supernatant was recovered after centrifugation to prepare a fermented Keumhwagyu.

Example 1. Characterization of nano-treated fermented Kumhwa silica

(1) Measurement of sugar content and pH

The sugar content and pH of the nano-treated fermented Kumhwa silicon were measured, respectively. The sugar content was expressed by measuring the total soluble solids (Brix) content with a saccharometer (Hand refractormeter, Germany), and the pH was expressed as an average value measured three times using a pH meter (SevenCompact™ pH/Ion S220, Switzerland). It was.

As a result, it was confirmed that the nano-treated fermented silicon dioxide of the present invention exhibited different characteristics from that of the non-nanized fermented silica (Table 1).

pH and sugar content of fermented Keumhwa silica treated with nano experimental group pH sugar content Fermented Geumhwa Beef 8.78 6.8 Fermented Geumhwagyu fermented with nano-treatment for 2 hours 8.87 6.7 Fermented Geumhwagyu fermented with nano treatment for 4 hours 9.04 6.7

(2) Protein content measurement

The protein content in the nano-treated fermented Geumhwagyu was measured through a protein quantification method using bicinchoninic acid (BCA).

As a result, it was confirmed that the nano-treated fermented Kumhwa silicon of the present invention had a higher protein content than the nano-treated fermented Kumhwa silicon ( FIG. 1 ).

(3) Measurement of amino acid content

Amino acids (cystine, methionine, serine, glutamic acid, glycine, alanine, citrulline, isoleucine, leucine, phenylalanine, aminobutyric acid, histidine, ornithine) in the nano-treated fermented Geumhwasil through amino acid quantification method using ninhydrin , lysine, ammonia and arginine) were measured.

As a result, it was confirmed that the nano-treated fermented Kumhwa silicon of the present invention had a higher amino acid content than the nano-treated fermented Kumhwa silicon ( FIG. 2 ).

(4) Collagen content analysis

The collagen content in the nano-treated fermented Keumhwagyu was measured. 20 μl of 0, 15, 30, and 50 μg/ml collagen and non-nanized fermented or nano-treated fermented gold whammys were dispensed in a black flat-bottom 96-well plate, respectively, and then digested. 30 μl of Mix was added, mixed, wrapped in foil, and reacted at 37° C. for 60 minutes. Then, 40 μl of dyeing reagent is added and reacted at 37° C. for 10 minutes, 10 μl of developer is added and reacted at 37° C. for 10 minutes, and the absorption wavelength is 375 nm and the emission wavelength is 465 nm under the condition that The fluorescence intensity was measured and calculated according to the following formula.

As a result, it was confirmed that the nano-treated fermented Kumhwa silicon of the present invention had a higher collagen content than the nano-treated fermented Kumhwa silicon ( FIG. 3 ).

Example 2. Antioxidant activity assay

(1) DPPH free radical scavenging activity analysis

Antioxidant activity was analyzed according to the method of Blois et al. After dissolving 16 mg of DPPH (2,2-diphenyl-1-picryl-hydrazyl) in 100 ml of ethanol, 100 ml of distilled water was mixed and filtered through Whatman filter paper (NO.2) to prepare a DPPH reaction solution. For DPPH free radical scavenging activity, add 4.6 ml of the DPPH reaction solution to 0.4 ml of each of the fermented fermented fermented gold wharf or fermented fermented fermented komhwa silica that has not been nano-treated, mixed well, and reacted in the dark for 30 minutes with a spectrophotometer (Hitachi U- 2900, Japan) was used to measure the absorbance at 528 nm, and then substituted into the equation below to calculate it. At this time, synthetic antioxidant 0.1% BHT (Butylated hydroxytoluene) was used as a positive control.

DPPH free radical scavenging activity (%)=[1-(A/B)×100]

A: Absorbance of sample-added DPPH solution at 528 nm

B: Absorbance of sample-free DPPH solution at 528 nm

As a result, it was confirmed that the nano-treated fermented silicon dioxide of the present invention had better free radical scavenging ability than the non-nanized fermented silicon dioxide (FIG. 4).

(2) Analysis of polyphenol and flavonoid content

Polyphenol (polyphenol) is one of the representative antioxidants, it protects DNA damaged by exposure to free radicals, and protects cell constituent proteins and enzymes. Flavonoids are plant-derived polyphenol-based compounds and are known to have antioxidant, anti-inflammatory, and anti-cancer effects.

The content of polyphenols and flavonoids in the nano-treated Keumhwagyu fermented product was measured using a total phenolic compounds measuring assay and a total flavonoids compounds measuring assay.

As a result, it was confirmed that the nano-treated fermented Kumhwa silicon of the present invention had a higher polyphenol and flavonoid content than the non-nanized fermented Kumhwa silicon ( FIG. 5 ).

From the above results, it can be seen that the nano-treated fermented gold Hwa silicon of the present invention has superior antioxidant activity than the nano-treated fermented Keumhwa silicon.

Example 3. Anti-inflammatory activity assay

Lipoxygenase (lipoxygenase) has insufficient activity in normal cells, but increases in activity due to an increase in leukotrienes when inflammation is induced. After adding 1 ml of 200 mM borate buffer (pH 9.0) to 100 μl of each 100 μl of the fermented fermented fermented fermented gold wharf or nano-treated fermented fermented gold whammy, linoleic acid as a substrate (linoleic acid, 0.6 mM/ml in ethanol) After mixing by adding 2 ml, the correction value was measured at 234 nm. Thereafter, 100 μl of 10,000 unit/ml lipoxygenase was added to each of the fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented gold hwasil at room temperature at room temperature for 10 minutes, and then the absorbance was measured at 234 nm. As a positive control, 0.1% indomethacin (IDM) was used, and the lipoxygenase inhibitory activity (%) was calculated by the following formula.

Lipoxygenase inhibitory activity (%)={1-(b'-b)/(a'-a)}×100

a: enzyme-free group

a': enzyme addition group

b: enzyme-free and sample-added group

b': enzyme and sample addition group

As a result, it was confirmed that the nano-treated fermented Kumhwa silica of the present invention had superior lipoxygenase inhibitory activity than the fermented Kumhwa silicon that was not nano-treated, and had superior lipoxygenase inhibitory activity than the positive control 0.1% indomethacin. By confirming that, it was found that the anti-inflammatory effect of the nano-treated fermented Kumhwa silica was enhanced (FIG. 6).

Example 4. Analysis of skin wrinkle improvement activity

Elastase inhibitory activity was measured to analyze the skin whitening activity of the nano-treated fermented Geumhwagyu fermented product of the present invention. 100 μl and 0.12M Tris-, respectively, of the non-nanized fermented Kumhwagyu or the nano-treated fermented material of the present invention in 1,300 μl of N-succinyl-Ala-Ala-Ala-p-nitroanilide, which is an elastase-specific substrate. 100 μl of HCl (pH 8.0) was mixed and dispensed in a 96-well plate, 100 μl of elastase was dispensed every 20 seconds, reacted at room temperature for 10 minutes, and absorbance was measured at 410 nm at 20 second intervals. Elastase inhibitory activity was calculated by substituting the following formula. As a positive control, 0.1% ursolic acid, known as an elastase inhibitor, was used.

Elastase inhibitory activity (%) = [1- (A / B)] × 100

A: absorbance of the sample treatment group

B: Absorbance of the sample untreated group

As a result, it was confirmed that the nano-treated fermented Keumhwagyu silicate of the present invention had superior elastase inhibitory activity than the non-nanized fermented Keumhwasil, and it was found that the elastase inhibitory activity was superior to that of the positive control, 0.1% ursolic acid. By confirming, it was found that the nano-treated fermented gold ginseng had skin wrinkle-improving activity (FIG. 7).

Example 5. Skin whitening activity assay

In order to analyze the skin whitening activity of the nano-treated fermented Geumhwagyu of the present invention, the mushroom tyrosinase inhibitory activity was measured in vitro . In a 1.5 ml plastic cuvette, 0.3 ml of 2.5 mM L-DOPA (3,4-dihydroxyphenylalanine), 0.1 ml of non-nanized or nano-treated fermented Keumhwagyu and 0.1 M phosphate buffer solution ( pH 6.8) was mixed and reacted at 25°C. Thereafter, 0.05 ml of 1,380 units/ml mushroom tyrosinase (Sigma, USA) was added, reacted at 25° C. for 2 minutes, and absorbance was measured at 470 nm using a spectrophotometer. calculated. As a control, a sample solution containing no sample was used, and as a positive control, 0.1% arbutin, known as a tyrosinase inhibitor, was used.

Tyrosinase inhibitory activity (%)=100-[(A-B)/A]×100

A: The difference in absorbance between 0.5 and 1 minute of the reaction solution without the sample

B: The difference in absorbance between 0.5 and 1 minute of the reaction solution containing the sample

As a result, it was confirmed that the nano-treated fermented Kumhwa silicon of the present invention has superior tyrosinase inhibitory activity than the non-nanized fermented Kumhwa silicon, indicating that the nano-treated fermented Kumhwa silicon has skin whitening activity. There was (Fig. 8).

Claims (7)

A health functional food composition for antioxidant, anti-inflammatory, skin wrinkle improvement or skin whitening containing nano-treated fermented Geumhwagyu as an active ingredient. [Claim 2] The health functional food composition for antioxidant, anti-inflammatory, skin wrinkle improvement or skin whitening according to claim 1, wherein the Keumhwagyu is Keumhwagyu flower. According to claim 1, wherein the nano-treated fermented Geumhwagyu flower powder is inoculated with a Bacillus subtilis culture solution and fermented at 20-40° C. for 1 to 5 days, and then fine beads (beads) in a nanoization device. ) for antioxidant, anti-inflammatory, skin wrinkle improvement, or skin whitening health functional food composition, characterized in that it is nano-treated at 30-50° C. for 1 to 5 hours. The method of claim 1, wherein the nano-treated fermented gold whammy fermented product has protein content, amino acid content, collagen content, polyphenol content, flavonoid content, antioxidant activity, anti-inflammatory activity, skin wrinkle improvement effect compared to non-nanized fermented gold wharf fermented product. Or a health functional food composition for antioxidant, anti-inflammatory, skin wrinkle improvement or skin whitening, characterized in that the skin whitening effect is enhanced. A cosmetic composition for antioxidant, anti-inflammatory, skin wrinkle improvement or skin whitening containing nano-treated fermented Geumhwagyu as an active ingredient. (a) mixing and sterilizing water, Keumhwagyu flower powder and glucose;
(b) inoculating and fermenting the Bacillus subtilis strain culture solution in the sterilized solution of Keumhwagyu flowers of step (a); and
(c) nano-treating the fermented product of step (b); a method for producing a nano-treated fermented gold fermented product with enhanced antioxidant activity, anti-inflammatory activity, skin wrinkle improvement and skin whitening effect, comprising a. 7. The method of claim 6,
(a) mixing 0.5 to 1.5 parts by weight of Keumhwagyu flower powder and 0.5 to 1.5 parts by weight of glucose with respect to 100 parts by weight of water and sterilizing;
(b) inoculating the Bacillus subtilis strain culture solution in the sterilized solution of Keumhwagyu flowers of step (a) at a concentration of 5 to 15% (v/v), and fermenting at 20 to 40° C. for 1 to 5 days ; and
(c) nano-processing the fermented product of step (b) at 30-50° C. for 1 to 5 hours using fine beads in a nano-device; antioxidant activity, anti-inflammatory activity, including skin wrinkle improvement and a method for producing a nano-treated fermented Geumhwagyu with enhanced skin whitening effect.

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