KR20220117076A - Container for preserving specimen, and kit for sampling and preserving of a specimen comprising the same - Google Patents

Abstract

The present invention relates to a container for storing a specimen which comprises a head unit, a body unit, and a bottom unit including a plurality of protrusions, wherein an internal space accommodating a specimen and liquid medium is formed. In addition, the container for storing a specimen has a hydrophobic coating layer formed of a hydrophobic composition containing hydrophobic silica and polytetrafluoroethylene in the internal space, thereby minimizing adhesion of the specimen and liquid medium to the internal space. Moreover, according to the present invention, provided is a kit for collecting and storing specimen which can suppress loss of the specimen and liquid medium and realize excellent screening efficiency in a specimen diagnostic test requiring high precision as the container for storing a specimen is included.

Description

Specimen storage container and kit for sample collection and storage including same

The present invention relates to a sample storage container and a kit for collecting and storing a sample comprising the same.

In general, sample collection is performed to test microorganisms including bacteria for the purpose of hygiene and quarantine, etc. In order to obtain accurate test results, it is preferable to test immediately after collecting the sample, but in general, the collection of the sample Immediately afterward, there is a practical difficulty in conducting the test immediately, so most of the collected samples are preserved in an appropriate environment and transported to related institutions where microbiological tests can be performed.

Accordingly, the sample is transported in a state that is accommodated in a preservable container, and the storage container in which the sample is stored is usually composed of a plastic tube containing a medium for preserving the collected sample, and a plastic stopper combined with a sterilized disposable cotton swab. The transport medium container type is mainly used.

For example, in No. 20-2000-0020092 for the Republic of Korea Registration Office, a swab comprising a swab head and a swab rod is attached to the lower end of the stopper; And, the middle part of the cylindrical body is recessed inward to have a pressing surface, the distance between the pressing surfaces is slightly larger than the diameter of the swab head, and when pressure is applied to the pressing surface, the distance between the pressing surfaces is the swab head It is disclosed for a medium container for sample collection and transport, including; a body made of an elastic material that is smaller than the diameter of the. In addition, most of the specimen storage containers, including those disclosed in the registration room, are manufactured using general plastic materials such as polyethylene, polypropylene, polycarbonate, and polystyrene.

On the other hand, in the internal space of the specimen storage container, a liquid medium is mainly accommodated for the purpose of microbial growth, biochemical testing, detection of metabolites, and the like. The liquid medium is prepared by dissolving specific components in distilled water, and as distilled water is the main component, the liquid medium containing distilled water as the main component and the sample contained in the liquid medium are attached to the inside of the sample storage container that is not hydrophobized, There was a problem in that the recovery efficiency of the specimen from the specimen storage container was lowered.

Accordingly, the applicant of the present applicant formed a hydrophobic coating layer inside the sample storage container, so that a liquid medium containing distilled water as a main component and a sample contained in the liquid medium are attached to the inside of the sample storage container and can be generated. By minimizing degradation, the present invention was accomplished.

Republic of Korea Registered Utility No. 20-2000-0020092

The first object of the present invention is to perform a hydrophobization treatment in the internal space in which the specimen is accommodated, so that a liquid medium containing distilled water as a main component and a sample contained in the liquid medium are attached to the inside of the specimen storage container and are generated, screening efficiency It is to provide a sample storage container that can minimize the deterioration of

A second object of the present invention is to provide a sample collection and storage kit including the sample storage container and having excellent sample collection and storage efficiency.

The object of the present invention is not limited to the technical problem as described above, and another technical problem may be derived from the following description.

In order to achieve the first object, in the present invention a head portion; In the specimen storage container comprising: a body and a bottom; and having an internal space for accommodating a specimen and a liquid medium, the bottom includes a plurality of rod-shaped protrusions protruding upward from an upper surface of the bottom, , A hydrophobic coating layer formed of a hydrophobic composition comprising hydrophobic silica and polytetrafluoroethylene (PTFE) is formed on the head, body and bottom, wherein the hydrophobized silica has an average particle diameter of 1 to 20 micrometers. It provides a sample storage container in which the surfaces of the phosphorus first silica and the second silica having an average particle diameter of 80 to 110 micrometers are hydrophobized.

The head part, the body part, and the bottom part may be formed of a material including at least one selected from the group consisting of polystyrene (PS), polypropylene (PP), and polycarbonate (PC).

The hydrophobized silica is heptadecafluoro-1,1,2,2-tetrahydrodecyl on the surface of the first silica having an average particle diameter of 5 to 10 micrometers and the second silica having an average particle diameter of 90 to 100 micrometers. The related may be combined and hydrophobized.

The first silica having an average particle diameter of 5 to 10 micrometers and the second silica having an average particle diameter of 90 to 100 micrometers may be included in a weight ratio of 1:0.1 to 1.

The hydrophobic composition may include 30 to 50 parts by weight of the hydrophobized silica based on 100 parts by weight of the polytetrafluoroethylene.

The hydrophobic coating layer may have a thickness of 150 micrometers to 1 millimeter.

The hydrophobic composition further includes a dispersing agent, and may include 5 to 10 parts by weight of the dispersant based on 100 parts by weight of the polytetrafluoroethylene.

In order to achieve the second object, the present invention provides a sample collection and storage kit including the sample storage container.

The specimen storage container of the present invention can minimize the phenomenon that the specimen and liquid medium are attached to the space inside the specimen storage container. Accordingly, in a specimen diagnostic test requiring high precision, loss of the specimen and liquid medium is suppressed, and thus the examination efficiency may be improved.

In addition, the specimen collection and storage kit of the present invention includes the specimen storage container, thereby stably storing and transporting the collected specimen, and suppressing the loss of the specimen and liquid medium, thereby improving screening efficiency.

1 is a diagram illustrating a projected perspective view of a specimen storage container according to an embodiment of the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily carry out the present invention. However, the present invention may be embodied in several different forms and is not limited to the embodiments described herein. And in order to clearly explain the present invention in the drawings, parts irrelevant to the description are omitted.

The terms or words used in the specification and claims of the present invention are not to be construed as limited in their ordinary or dictionary meanings, and the inventor may appropriately define the concept of the term in order to best describe his invention. Based on the principle, it should be interpreted as meaning and concept consistent with the technical idea of the present invention.

In the entire specification of the present invention, when a part "includes" a certain component, it means that other components may be further included, rather than excluding other components unless otherwise stated. .

Throughout the specification of the present invention, "A and/or B" means A or B, or A and B.

Although the present invention has been specifically described below, the present invention is not limited thereto.

The present invention provides a specimen storage container.

In an embodiment of the present invention, the head portion 10; body 20; and a bottom portion 30; and provides a specimen storage container 100 having an internal space for accommodating the specimen and the liquid medium.

Specifically, in the specimen storage container 100 of this embodiment, the bottom 30 includes a plurality of rod-shaped protrusions 40 protruding upward from the top surface of the bottom 30, and the A hydrophobic coating layer formed of a hydrophobic composition including hydrophobized silica and polytetrafluoroethylene (PTFE) is formed on the head 10 , the body 20 and the bottom 30 , but the hydrophobized silica is average The surface of the first silica having a particle diameter of 1 to 20 micrometers and the second silica having an average particle diameter of 80 to 110 micrometers may be hydrophobized.

Accordingly, the specimen storage container 100 of the present embodiment can minimize the phenomenon that the specimen and liquid medium are attached to the internal space of the specimen storage container 100, and thus, in the specimen diagnostic test requiring high precision, the specimen And as the loss of the liquid medium is suppressed, the screening efficiency can be improved.

Hereinafter, the components of the specimen storage container 100 will be described in detail.

As shown in FIG. 1 , the specimen storage container 100 of this embodiment includes a head portion 10 , a body portion 20 , and a bottom portion 30 including a plurality of protrusions 40 , and the head It is formed by the combination of the part 10 , the body part 20 , and the bottom part 30 , and may include an internal space for accommodating a sample and a liquid medium.

The specimen storage container 100 of this embodiment is not limited to that shown in FIG. 1 , and includes a head portion 10 , a body portion 20 , and a bottom portion 30 including a plurality of protrusions 40 , If it is a form including an internal space formed by the combination thereof, it is not particularly limited.

The head part 10 of this embodiment is a passage connecting the internal space of the specimen storage container 100 and the external space of the specimen storage container 100 , and may be formed in a form that can be opened and closed. For example, as shown in FIG. 1 , the head part 10 of the present embodiment may be formed in the form of a lid that covers and surrounds the upper side of the body part 20 , but is not limited thereto.

The body 20 of this embodiment is a main part forming the inner space of the specimen storage container 100 and may be formed in a cylindrical shape. At this time, the difference between the inner diameter of the cylinder and the outer diameter of the cylinder may be formed within 10 mm, but is not limited thereto.

At this time, as shown in FIG. 1 , if the head part 10 is formed on the upper part of the body part 20 , and the head part 10 can open and close the upper part of the body part 20 , It is not particularly limited. For example, the head portion 10 may be formed in the form of a lid having a circular cross-section in order to be formed to cover and surround the upper portion of the cylindrical body portion 20 .

The bottom part 30 of this embodiment is formed on the opposite side of the head part 10 , and may be formed integrally with the body part 20 , and is formed in the same shape as the horizontal cross-section of the body part 20 . can be For example, the bottom 30 of the present embodiment may be formed in a circular plate shape as shown in FIG. 1 , but is not limited thereto.

The bottom part 30 of this embodiment may include a plurality of protrusions 40 that protrude upward and are formed in a bar shape when the inner space side is the upper surface of the bottom part 30 . In this case, the plurality of protrusions 40 may be disposed to be spaced apart from each other by a predetermined interval, and may collide with the cotton swab on which the collected specimen is buried, so that the specimen is easily detached from the cotton swab.

To be specific, in general, when a cotton swab with a collected sample is placed in the inner space of the sample storage container 100 containing the liquid medium, the sample attached to the cotton swab falls into the liquid medium, at this time, As the swab collides with the plurality of protrusions 40 of the present embodiment, the specimen attached to the swab may easily come off into the liquid medium.

The head part 10, the body part 20 and the bottom part 30 of this embodiment are made of a material containing at least one selected from the group consisting of polystyrene (PS), polypropylene (PP) and polycarbonate (PC). It may be formed, but is not limited thereto.

Specifically, the head portion 10, the body portion 20, and the bottom portion 30 of the present embodiment may be formed of polystyrene, but is not limited thereto.

As shown in FIG. 1, the specimen storage container 100 of this embodiment includes a head part 10, a body part 20 and a bottom part 30, and by combining them, the specimen and the liquid medium are stored. An interior space that can be accommodated is formed.

At this time, in the process of recovering the liquid medium containing the sample from the sample storage container 100 of this embodiment, some of the sample and the liquid medium remain in the inner space of the sample storage container 100, so that the sample diagnostic test There was a problem in that the efficiency of the Accordingly, in this embodiment, by forming a hydrophobic coating layer in the internal space of the specimen storage container 100, the adhesion between the internal space of the specimen storage container 100 and a liquid medium containing distilled water as a main component is lowered, By increasing the slip property, it is possible to minimize the above problems.

The hydrophobic coating layer of this embodiment may be formed of a hydrophobic composition including hydrophobized silica and polytetrafluoroethylene (PTFE). Specifically, the hydrophobic coating layer of this embodiment may mean a film formed by coating or spraying a hydrophobic composition containing silica and polytetrafluoroethylene (PTFE), and drying or curing it.

The hydrophobized silica may mean that the surface of the first silica having an average particle diameter of 1 to 20 micrometers and the second silica having an average particle diameter of 80 to 110 micrometers is hydrophobized. In particular, in this embodiment, by including two types of silica having different average particle diameters, hydrophobicity can be further improved.

On the other hand, when silica having an average particle diameter of nanometer level is used, there is a problem in that silica agglomeration occurs. Accordingly, in this embodiment, by using silica having an average particle diameter of a micrometer level, aggregation of silica was minimized, so that uniform hydrophobicity could be implemented on the entire surface of the hydrophobic coating layer.

Specifically, the hydrophobized silica is heptadecafluoro-1,1,2,2-tetra on the surface of the first silica having an average particle diameter of 5 to 10 micrometers and the second silica having an average particle diameter of 90 to 100 micrometers. Hydrodecyl silylate may be bound and hydrophobized, and in this case, hydrophobicity may be further improved.

More specifically, the hydrophobized silica is a mixture of a first silica having an average particle diameter of 5 to 10 micrometers and a second silica having an average particle diameter of 90 to 100 micrometers in a weight ratio of 1: 0.1 to 1, and hepta on the surface thereof. Decafluoro-1,1,2,2-tetrahydrodecylsilylate may be combined and hydrophobized, and in this case, hydrophobicity may be significantly improved.

Most specifically, the hydrophobized silica is a mixture of a first silica having an average particle diameter of 5 to 10 micrometers and a second silica having an average particle diameter of 90 to 100 micrometers in a weight ratio of 1: 0.2 to 0.3, and hepta on the surface thereof. Decafluoro-1,1,2,2-tetrahydrodecylsilylate may be combined and hydrophobized, and in this case, hydrophobicity may be remarkably excellent.

The hydrophobic composition of this embodiment may include 30 to 50 parts by weight of the hydrophobized silica, specifically 40 to 50 parts by weight, more specifically 45 to 47 parts by weight based on 100 parts by weight of the polytetrafluoroethylene. .

The hydrophobic composition of this embodiment contains 30 to 50 parts by weight of the hydrophobized silica with respect to 100 parts by weight of the polytetrafluoroethylene, thereby having a stable and uniform thickness, and excellent properties such as water resistance, chemical resistance, durability, It is possible to form a coating layer in the form of a film. In particular, since the hydrophobic composition of this embodiment contains 45 to 47 parts by weight of the hydrophobized silica based on 100 parts by weight of the polytetrafluoroethylene, the above effect may be more excellent.

If, in the hydrophobic composition of this embodiment, the hydrophobized silica is included in an amount of less than 30 parts by weight based on 100 parts by weight of the polytetrafluoroethylene, the degree of improvement in hydrophobicity is insignificant, and a liquid medium containing distilled water as a main component. and the sample contained in the liquid medium, it may be difficult to solve a problem caused by being attached to the inside of the sample storage container 100 . On the other hand, in the hydrophobic composition of this embodiment, when the hydrophobized silica is included in an amount of more than 50 parts by weight based on 100 parts by weight of the polytetrafluoroethylene, transparency is lowered, and it is difficult to form a film having a stable and uniform thickness. , the overall performance may be degraded.

The hydrophobic coating layer in the form of a film formed of the hydrophobic composition of this embodiment may have a thickness of 150 micrometers to 1 mm, specifically 200 micrometers to 500 micrometers, and more specifically 250 micrometers to 400 micrometers.

If the thickness of the hydrophobic coating layer of this embodiment is less than 150 micrometers, the degree of improvement in hydrophobicity may be insignificant. On the other hand, when the thickness of the hydrophobic coating layer of this embodiment is more than 1 mm, transparency is lowered, and the degree of hydrophobicity improvement effect compared to the amount of the hydrophobic composition used is insignificant, which may not be economical.

The hydrophobic composition of this embodiment may further include a dispersing agent.

Specifically, the dispersant may include a polyoxyethylene alkyl ether-based nonionic surfactant.

More specifically, the dispersant may include polyoxyethylene cetyl ether and polyoxyethylene stearyl ether in a weight ratio of 1:5, and in this case, the dispersibility of the hydrophobized silica is remarkably excellent, so that the properties of the hydrophobic coating layer are improved. can be further improved.

The hydrophobic composition of this embodiment may include 5 to 10 parts by weight of a dispersant based on 100 parts by weight of the polytetrafluoroethylene. In particular, when the hydrophobic composition of this embodiment contains 5 to 10 parts by weight of a dispersing agent based on 100 parts by weight of the polytetrafluoroethylene, the degree of dispersion of the hydrophobized silica in the hydrophobic coating layer is uniform and stable and the thickness is uniform. can

The hydrophobic composition of this embodiment contains at least one selected from the group consisting of solvents, surfactants, emulsifiers, stabilizers, antioxidants, anti-aging agents, defoamers, chelating agents and preservatives, in addition to hydrophobized silica, polytetrafluoroethylene and dispersants. It may further include additives including

In addition, in this embodiment, a kit for collecting and storing a specimen including the specimen storage container 100 is provided. The sample collection and storage kit may further include all known items necessary for sample collection, storage, transportation, etc. in addition to the sample storage container 100 .

Hereinafter, the specimen storage container of the present invention will be described in detail through Examples, Comparative Examples, and Experimental Examples. These examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention by these examples.

[Example]

Examples 1-3

A hydrophobic composition was prepared by mixing 45 parts by weight of hydrophobized silica and 5 parts by weight of a dispersant with respect to 100 parts by weight of polytetrafluoroethylene (PTFE).

Then, by applying and curing the hydrophobic composition on a plate formed of polystyrene, a hydrophobic coating layer having a thickness of 250 micrometers to 400 micrometers was formed.

At this time, the hydrophobized silica is mixed with the first silica and the second silica according to Table 1 [unit: weight ratio], and heptadecafluoro-1,1,2,2- Tetrahydrodecyl silylate was bound and hydrophobized was used.

Specifically, the hydrophobized silica was prepared as follows.

First, according to Table 1, in a suspension of 30 ml of ethanol containing 0.5 g of silica mixed particles in which the first silica and the second silica are mixed, heptadecafluoro-1,1,2,2-tetra purchased from Gelest Inc. A mixture of 0.14 g (0.25 mmol) of hydrodecytrimethoxysilane (HFTHTMS), 0.02 ml of aqueous ammonia and 0.36 ml of deionized water was added, refluxed for 20 hours, and then centrifuged at 2000 rpm at room temperature for 30 minutes. was precipitated by Then, the precipitate was ultrasonically washed with ethanol and then centrifuged at 2000 rpm for 30 minutes to finally bind to heptadecafluoro-1,1,2,2-tetrahydrodecylsilylate to obtain hydrophobized silica. did.

In addition, as a dispersant, a mixture of polyoxyethylene cetyl ether and polyoxyethylene stearyl ether in a weight ratio of 1:5 was used.

Example 1 Example 2 Example 3 first silica One One One secondary silica 0.1 0.3 One *First silica: silica having an average particle diameter of 5 to 10 micrometers
*Second silica: silica having an average particle diameter of 90 to 100 micrometers

[ Comparative Example ]

Comparative Example 1

It was carried out in the same manner as in Example 1, except that only the first silica was used (using the second silica X) to prepare a hydrophobized silica, and a hydrophobic composition was formed using this.

Comparative Example 2

It was carried out in the same manner as in Example 1, except that only the second silica was used (using the first silica X) to prepare a hydrophobized silica, and a hydrophobic composition was formed using this.

Comparative Examples 3 to 4

It was carried out in the same manner as in Example 1, except that it was prepared by mixing the hydrophobic composition according to Table 2 [unit: parts by weight].

Example 1 Comparative Example 3 Comparative Example 4 polytetrafluoroethylene 100 100 100 Hydrophobized Silica 45 20 60 dispersant 5 5 5

[Experimental example]

Experimental Example 1: Hydrophobicity evaluation

A water drop was dropped on the hydrophobic coating layer formed according to Examples 1 to 3 and Comparative Examples 1 to 4 to measure the contact angle between the coating film and the water drop, and the results are shown in Table 3 [unit: °].

At this time, the contact angle was measured using a Kruss DSA10 (Germany) contact angle measuring device, after dropping 3 μl of water droplets on the hydrophobic coating layer, adding 8 μl of water and recording the contact angle measured for 100 seconds. did.

In addition, for comparison with the hydrophobic coating layer of Examples and Comparative Examples, using a general polystyrene plate that does not include a hydrophobic coating layer as an untreated group, the above experiment was conducted in the same manner, and the results are shown in Table 3 as 'untreated. marked as 'gun'.

contact angle contact angle Example 1 115.6 Comparative Example 1 94.3 Example 2 127.4 Comparative Example 2 96.5 Example 3 112.4 Comparative Example 3 90.1 untreated group 85.4 Comparative Example 4 108.4

Referring to Table 3, it can be seen that, according to this embodiment, the water droplet contact angle is remarkably excellent as 110° or more, thereby forming a coating layer having excellent hydrophobic properties.

On the other hand, in the case of Comparative Example 4, although the contact angle was excellent, the hydrophobic coating layer was formed opaque enough to be difficult to observe inside, and despite the excessive use of hydrophobized silica, the degree of improvement in hydrophobicity was not excellent compared to that of Example.

[ Manufacturing Example ]

Preparation Example 1

A storage container made of a polystyrene material and having a shape as shown in FIG. 1 was prepared.

Then, the hydrophobic coating layer of Example 2 was formed in the same manner on the head portion, the body portion, and the bottom portion forming the internal space of the specimen storage container, thereby preparing a specimen storage container according to the present invention.

[Experimental example]

Experimental Example 2: Visual observation of residues

After the known liquid medium was put into Preparation Example 1 (the sample storage container having the hydrophobic coating layer of Example 2), it was left for about 1 hour. Thereafter, after removing the liquid medium (containing distilled water) from the sample storage container, it was left at room temperature for about 10 minutes, and then a tissue was placed in the sample storage container, and the degree of spillage of the liquid medium was visually observed.

In addition, for comparison with Preparation Example 1, the experiment was conducted in the same manner using a known specimen storage container (polystyrene material) in which the hydrophobic coating layer was not formed.

As a result, in the case of the tissue wiping the sample storage container of Preparation Example 1 with the hydrophobic coating layer formed according to this example, the liquid medium was smeared to less than 20% of the total area, but a general sample storage container in which the hydrophobic coating layer was not formed. In the case of the wiped tissue, it was confirmed that the liquid medium was smeared on 40 to 60% of the total area.

That is, in view of the above results, according to the present embodiment, the specimen storage container with the hydrophobic coating layer can minimize the phenomenon of the specimen and liquid medium adhering to the inner space of the specimen storage container, thereby reducing the loss of the specimen and liquid medium. As it is suppressed, screening efficiency can be improved.

As described above, the description of the present invention is for illustration, and those of ordinary skill in the art to which the present invention pertains can easily transform into other specific forms without changing the technical spirit or essential features of the present invention. You will understand. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. For example, each component described as a single type may be implemented in a dispersed form, and likewise components described as distributed may also be implemented in a combined form.

The scope of the present invention is indicated by the following claims rather than the above detailed description, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be interpreted as being included in the scope of the present invention. do.

100: sample storage container
10: head part
20: body
30: bottom
40: turn

Claims (8)

head; body part; and a bottom portion, wherein the specimen storage container is formed with an internal space for accommodating the specimen and the liquid medium,
The bottom part includes a plurality of rod-shaped protrusions protruding upward from the top surface of the bottom part,
The head part, the body part and the bottom part,
A hydrophobic coating layer formed of a hydrophobic composition comprising hydrophobized silica and polytetrafluoroethylene (PTFE) is formed,
The hydrophobized silica is
A specimen storage container, characterized in that the surfaces of the first silica having an average particle diameter of 1 to 20 micrometers and the second silica having an average particle diameter of 80 to 110 micrometers are hydrophobized.
The method of claim 1,
The head part, the body part and the bottom part
A specimen storage container, characterized in that it is formed of a material comprising at least one selected from the group consisting of polystyrene (PS), polypropylene (PP) and polycarbonate (PC).
The method of claim 1,
The hydrophobized silica is
Heptadecafluoro-1,1,2,2-tetrahydrodecyl silylate is bonded to the surface of the first silica having an average particle diameter of 5 to 10 micrometers and the second silica having an average particle diameter of 90 to 100 micrometers. , The specimen storage container, characterized in that it is hydrophobized.
4. The method of claim 3,
The sample storage container, characterized in that the first silica having an average particle diameter of 5 to 10 micrometers and the second silica having an average particle diameter of 90 to 100 micrometers are included in a weight ratio of 1:0.1 to 1.
The method of claim 1,
The hydrophobic composition is
The sample storage container, characterized in that it contains 30 to 50 parts by weight of the hydrophobized silica based on 100 parts by weight of the polytetrafluoroethylene.
The method of claim 1,
The thickness of the hydrophobic coating layer is 150 micrometers to 1 mm, characterized in that the sample storage container.
The method of claim 1,
The hydrophobic composition further comprises a dispersant,
The sample storage container, characterized in that it contains 5 to 10 parts by weight of the dispersant based on 100 parts by weight of the polytetrafluoroethylene.
A kit for collecting and storing a specimen comprising the specimen storage container of claim 1.

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