CN109055206A - A kind of antimicrobial sheet material antibacterial surface and bacteriostasis property test device and its method - Google Patents
A kind of antimicrobial sheet material antibacterial surface and bacteriostasis property test device and its method Download PDFInfo
- Publication number
- CN109055206A CN109055206A CN201811250005.4A CN201811250005A CN109055206A CN 109055206 A CN109055206 A CN 109055206A CN 201811250005 A CN201811250005 A CN 201811250005A CN 109055206 A CN109055206 A CN 109055206A
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- shell
- sheet material
- bacterium
- bacteriostasis property
- test device
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- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 47
- 238000012360 testing method Methods 0.000 title claims abstract description 43
- 239000000463 material Substances 0.000 title claims abstract description 39
- 230000000845 anti-microbial effect Effects 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title abstract description 9
- 241000894006 Bacteria Species 0.000 claims abstract description 43
- 238000002474 experimental method Methods 0.000 claims abstract description 31
- 239000007787 solid Substances 0.000 claims abstract description 18
- 239000001963 growth medium Substances 0.000 claims abstract description 15
- 238000001514 detection method Methods 0.000 claims abstract description 10
- 229940099259 vaseline Drugs 0.000 claims abstract description 7
- 238000010998 test method Methods 0.000 claims abstract description 5
- 230000001954 sterilising effect Effects 0.000 claims description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 238000009395 breeding Methods 0.000 claims description 5
- 230000001488 breeding effect Effects 0.000 claims description 5
- 238000005259 measurement Methods 0.000 claims description 4
- 230000008014 freezing Effects 0.000 claims description 3
- 238000007710 freezing Methods 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 238000009400 out breeding Methods 0.000 claims description 3
- 238000012546 transfer Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims 1
- 238000009408 flooring Methods 0.000 abstract 1
- 230000003115 biocidal effect Effects 0.000 description 5
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 3
- 239000002023 wood Substances 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- QCWXUUIWCKQGHC-UHFFFAOYSA-N Zirconium Chemical compound [Zr] QCWXUUIWCKQGHC-UHFFFAOYSA-N 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000002147 killing effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229910052726 zirconium Inorganic materials 0.000 description 2
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 241001330002 Bambuseae Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 238000005299 abrasion Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000007731 hot pressing Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000009659 non-destructive testing Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- GCLGEJMYGQKIIW-UHFFFAOYSA-H sodium hexametaphosphate Chemical compound [Na]OP1(=O)OP(=O)(O[Na])OP(=O)(O[Na])OP(=O)(O[Na])OP(=O)(O[Na])OP(=O)(O[Na])O1 GCLGEJMYGQKIIW-UHFFFAOYSA-H 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of antimicrobial sheet material antibacterial surface and bacteriostasis property test devices, including testing plate (5), experiment plate (5) upper surface is provided with shell (2), the inner wall of shell (2) and state experiment plate (5) upper surface and be located at shell (2) in part be provided with bacterium solid culture medium layer (3), shell (2) interior upper end is provided with spotlight (1) and electron microscopic lens head (6), micro- lens actuator (7) are provided on electron microscopic lens head (6), vertical direction is provided with graduated scale (8) in shell (2), it is sealed between the outer wall and experiment plate (5) surface of shell (2) by vaseline.The invention also discloses a kind of antimicrobial sheet material antibacterial surface and bacteriostasis property test methods.A kind of antimicrobial sheet material antibacterial surface of the invention and bacteriostasis property test device and its method, it does not need to carry out saw system to detection plate, operate extremely simple, test result is quick, can simultaneously from sterilize and antibacterial two in terms of overall merit flooring material anti-microbial property.
Description
Technical field
The present invention relates to a kind of antimicrobial sheet material antibacterial surfaces and bacteriostasis property test device and its method, belong to plate detection
Field.
Background technique
According to wooden (bamboo) quality plate anti-microbial property detection method of professional standard LY/T 1926-2010 antibacterial and antibacterial effect,
Its detection method mainly includes three aspects: (1) preparation of test specimen specifically includes specimen sawing and bacteria removing.(2) strain
Switching and culture；(3) three aspects of the calculating of antibiotic rate.Its principle is to be inoculated in sample with selected strain, simulate certainly
By the culture of certain time, observation under conditions of right boundary fungus growth, detected with the method for range estimation fungus growth by sample
Then the anti-microbial property of product is classified the bactericidal property of material.
In fact, according to sterilization and antibacterial principle, this method is merely capable of judgement material for the killing effect of strain,
Be unable to intuitive judgment its for strain depression effect.Typically for antibacterial judgement, usually by antibacterial ring test into
Row, still, the test of same antibacterial ring size can not judge surface for the killing effect of strain.Therefore often such individually examination
Test this aspect performance of the not accurate enough and comprehensive reaction material of result.If antibiotic rate and antibacterial ring test are done by several times, due to wanting
Failure test is done, then not can guarantee the test situation of the same sample, and to damage detection, has destruction to plate, and saw system
Get up to be more troublesome.The testing time is long in the conventional way for institute, and the final data of trivial operations, acquisition is not comprehensive enough, is only capable of reacting
The sterilization of plate surface or bacteriostasis property.
Summary of the invention
The technical problem to be solved by the present invention is to overcome prior art Dun defect, provide one kind and do not need to detection plate
The saw system of progress, operates extremely simple, and test result is quick, being capable of the overall merit floor in terms of sterilizing and is two antibacterial simultaneously
The antimicrobial sheet material antibacterial surface and bacteriostasis property test device and its method of the anti-microbial property of material.
In order to solve the above technical problems, the technical solution adopted by the present invention are as follows:
A kind of antimicrobial sheet material antibacterial surface and bacteriostasis property test device, including experiment plate, table on the experiment plate
Face is provided with shell, the inner wall of the shell and the experiment plate upper surface and the part being located in the shell is provided with
Bacterium solid culture medium layer, upper end is provided with spotlight and electron microscopic lens head, the electron microscope mirror in the shell
Micro- lens actuator is provided on head, the shell is interior to be provided with graduated scale, the outer wall of the shell and institute in vertical direction
It states and is sealed between experiment plate surface by vaseline.
The material of the shell is glass.
The shape of the shell is cylinder.
The range of the graduated scale is 120mm.
The outer diameter size of the shell is 100mm.
The height of the shell is 120mm.
A kind of antimicrobial sheet material antibacterial surface and bacteriostasis property test method, comprising the following steps:
S01, carries out breeding strain, and freezing is stand-by；
S02 carries out disinfection to experimental plate material to be measured surface and test device；
The strain cultivated in S01 is transferred and is cultivated by bacterium solid culture medium layer to sheet material to be measured surface by S03,
The inner wall area of shell will be completely covered in the area of bacterium solid culture medium layer；
S04 adds bacterium solid culture medium layer in the inner wall of shell, by the strain transfer cultivated in S01 and cultivates extremely
The inner wall of shell, breeding strain height are the upward one end distance in outer casing inner wall bottom end；
Then test device is statically placed in experiment plate surface to be measured by S05, surrounding and experiment plate outside test device
It is sealed between upper surface by vaseline, and passes through the original quantity of bacterium of electron microscopic lens head observation experiment plate surface；
S06, after a period of time, it is antibacterial height that measurement interior of shell bacterium, which grows up mobile distance, antibacterial
The bacteriostasis rate of height and the ratio calculation experiment plate surface of the original height of strain in S04；
S07, after a period of time, by the bacterium volume residual of electron microscopic device observation experiment plate surface, carefully
The original quantity of bacterium and the difference of bacterium volume residual are that bacterium kills quantity, and bacterium kills the ratio between number and the original quantity of bacterium
Value is the sterilizing rate of experiment plate surface.
In S04, breeding strain height is 120mm.
In S06, a period of time is specially 36h.
S07, a period of time are specially 36h.
Beneficial effects of the present invention: a kind of antimicrobial sheet material antibacterial surface provided by the invention and bacteriostasis property test device and
Its method changes the test method of traditional material bacteriostasis property, has more rapidly, and more intuitive, more accurate advantage can
The bactericidal property and bacteriostasis property of floor surface are tested simultaneously, and realize the non-destructive testing of floor sterilization and bacteriostasis property
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of antimicrobial sheet material antibacterial surface and bacteriostasis property test device of the present invention.
Appended drawing reference is as follows in figure: 1- spotlight, 2- shell, 3- bacterium solid culture medium layer, 4- bacterium, 5- experimental plate
Material, 6- electron microscopic lens head, the micro- lens actuator of 7-, 8- graduated scale.
Specific embodiment
The invention will be further described below in conjunction with the accompanying drawings, and following embodiment is only used for clearly illustrating the present invention
Technical solution, and not intended to limit the protection scope of the present invention.
Specific embodiment 1
A kind of antimicrobial sheet material antibacterial surface and bacteriostasis property test device, including experiment plate 5, test 5 upper surface of plate
It is provided with shell 2, the inner wall of shell 2 is provided with bacterium solid culture medium layer 3, tests 5 upper surface of plate and is located in shell 2
Part is provided with bacterium solid culture medium layer 3, and bacterium solid culture medium layer 3 is for cultivating bacterium 4.Upper end is provided in shell 2
Spotlight 1 and electron microscopic lens head 6, are provided with micro- lens actuator 7 on electron microscopic lens head 6, in vertical side in shell 2
To graduated scale 8 is provided with, sealed between the outer wall and experiment 5 surface of plate of shell 2 by vaseline.
Preferably, the material of shell 2 is glass, and shape is cylinder.The range of graduated scale 8 is 120mm.Shell 2
Outer diameter size is 100mm.The height of shell 2 is 120mm.
The invention also discloses a kind of antimicrobial sheet material antibacterial surface and bacteriostasis property test methods, comprising the following steps:
Step 1, carries out breeding strain, and freezing is stand-by.
Step 2 carries out disinfection to experimental plate material to be measured surface and test device.Wherein, experiment plate uses HTB-032
Type carries the fir wood floor of silver-colored di zirconium nano antibacterial agent, and HTB-032 type is carried silver-colored di zirconium nano antibacterial agent with matter
Amount is divided into 1% ratio and is mixed with the finishing coat of fir wood floor, and dispersing agent selection is calgon, and additive amount is antibacterial
The 10% of agent.The measurement of antibacterial surface and bacteriostasis property is carried out to the fir wood floor after smearing finishing coat and solidifying.
The strain cultivated in S01 is transferred and is cultivated to sheet material to be measured table by bacterium solid culture medium layer by step 3
The inner wall area of shell will be completely covered in face, the area of bacterium solid culture medium layer.
Step 4 adds bacterium solid culture medium layer in the inner wall of shell, by the strain transfer cultivated in S01 and culture
To the inner wall of shell, breeding strain height is the upward one end distance in outer casing inner wall bottom end, here specially 100mm.
Then test device is statically placed in experiment plate surface to be measured by step 5, surrounding and experiment outside test device
It is sealed between plate upper surface by vaseline, and passes through the original number of bacterium of electron microscopic lens head observation experiment plate surface
Amount.
Step 6, after 36h, it is antibacterial height, antibacterial height that measurement interior of shell bacterium, which grows up mobile distance,
The bacteriostasis rate of degree and the ratio calculation experiment plate surface of the original height of strain in S04.
Step 7 passes through the bacterium volume residual of electron microscopic device observation experiment plate surface, bacterium after 36h
Original quantity and the difference of bacterium volume residual are that bacterium kills quantity, and bacterium kills the ratio between number and the original quantity of bacterium
For the sterilizing rate for testing plate surface.
Table 1 is the antibacterial surface of solid wooden floor board and the test result of bacteriostasis property.
| Experiment number | Antibiotic rate/% | Antibacterial height/mm |
| 1 | 99.5 | 12 |
| 2 | 99.5 | 11 |
| 3 | 99.8 | 12 |
| 4 | 99.6 | 13 |
| 5 | 99.6 | 12 |
Table 1
Specific embodiment 2
The present embodiment is identical as specific embodiment 1, and difference is that it is the reinforcing containing nano silver that plate is tested in step 2
Timber floor, production method are to be impregnated into nano silver in the abrasion-proof paper on consolidated floor surface, then carry out hot pressing fitting, nano silver
Concentration in consolidated floor surface abrasion resistance paper is 40 μ g/g, and consolidated floor obtained is then carried out antibacterial surface and biocidal property
The test of energy.Table 2 is the antibacterial surface of solid wooden floor board and the test result of bacteriostasis property.
| Experiment number | Antibiotic rate/% | Antibacterial height/mm |
| 1 | 99.0 | 7 |
| 2 | 99.1 | 8 |
| 3 | 99.2 | 8 |
| 4 | 99.1 | 8 |
| 5 | 99.1 | 7 |
Table 2
The above is only a preferred embodiment of the present invention, it should be pointed out that: for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of antimicrobial sheet material antibacterial surface and bacteriostasis property test device, it is characterised in that: described including testing plate (5)
Experiment plate (5) upper surface is provided with shell (2), the inner wall of the shell (2) and the experiment plate (5) upper surface and position
It is provided with bacterium solid culture medium layer (3) in the part in the shell (2), the interior upper end of the shell (2) is provided with spotlight
(1) and electron microscopic lens head (6) micro- lens actuator (7), the shell, are provided on the electron microscopic lens head (6)
(2) it is provided with graduated scale (8) in vertical direction, leads between the outer wall of the shell (2) and experiment plate (5) surface
Cross vaseline sealing.
2. a kind of antimicrobial sheet material antibacterial surface according to claim 1 and bacteriostasis property test device, it is characterised in that: institute
The material for stating shell (2) is glass.
3. a kind of antimicrobial sheet material antibacterial surface according to claim 1 and bacteriostasis property test device, it is characterised in that: institute
The shape of shell (2) is stated as cylinder.
4. a kind of antimicrobial sheet material antibacterial surface according to claim 1 and bacteriostasis property test device, it is characterised in that: institute
The range for stating graduated scale (8) is 120mm.
5. a kind of antimicrobial sheet material antibacterial surface according to claim 1 and bacteriostasis property test device, it is characterised in that: institute
The outer diameter size for stating shell (2) is 100mm.
6. a kind of antimicrobial sheet material antibacterial surface according to claim 1 and bacteriostasis property detection device, it is characterised in that: institute
The height for stating shell (2) is 120mm.
7. a kind of antimicrobial sheet material antibacterial surface and bacteriostasis property test method, it is characterised in that: the following steps are included:
S01, carries out breeding strain, and freezing is stand-by；
S02 carries out disinfection to experimental plate material to be measured surface and test device；
The strain cultivated in S01 is transferred and is cultivated to sheet material to be measured surface, bacterium by bacterium solid culture medium layer by S03
The inner wall area of shell will be completely covered in the area of solid culture base；
S04 adds bacterium solid culture medium layer in the inner wall of shell, by the strain transfer cultivated in S01 and cultivates to shell
Inner wall, breeding strain height be the upward one end distance in outer casing inner wall bottom end；
Then test device is statically placed in experiment plate surface to be measured by S05, surrounding and table on experiment plate outside test device
It is sealed between face by vaseline, and passes through the original quantity of bacterium of electron microscopic lens head observation experiment plate surface；
S06, after a period of time, it is antibacterial height, antibacterial height that measurement interior of shell bacterium, which grows up mobile distance,
With the bacteriostasis rate of the ratio calculation experiment plate surface of the original height of strain in S04；
S07, after a period of time, by the bacterium volume residual of electron microscopic device observation experiment plate surface, bacterium is former
There is quantity and the difference of bacterium volume residual is that bacterium kills quantity, the ratio that bacterium kills between number and the original quantity of bacterium is
Test the sterilizing rate of plate surface.
8. a kind of antimicrobial sheet material antibacterial surface according to claim 1 and bacteriostasis property detection method, it is characterised in that:
In S04, breeding strain height is 120mm.
9. a kind of antimicrobial sheet material antibacterial surface according to claim 1 and bacteriostasis property detection method, it is characterised in that:
In S06, a period of time is specially 36h.
10. a kind of antimicrobial sheet material antibacterial surface according to claim 1 and bacteriostasis property detection method, it is characterised in that:
S07, a period of time are specially 36h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811250005.4A CN109055206A (en) | 2018-10-25 | 2018-10-25 | A kind of antimicrobial sheet material antibacterial surface and bacteriostasis property test device and its method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811250005.4A CN109055206A (en) | 2018-10-25 | 2018-10-25 | A kind of antimicrobial sheet material antibacterial surface and bacteriostasis property test device and its method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109055206A true CN109055206A (en) | 2018-12-21 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811250005.4A Pending CN109055206A (en) | 2018-10-25 | 2018-10-25 | A kind of antimicrobial sheet material antibacterial surface and bacteriostasis property test device and its method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113820337A (en) * | 2021-11-24 | 2021-12-21 | 深圳市丰用实业集团有限公司 | Machine vision-based antibacterial ecological plate production and manufacturing quality detection early warning system |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201309942Y (en) * | 2008-11-21 | 2009-09-16 | 王升 | Comb-shaped medicament susceptibility test scrip |
| JP2015177768A (en) * | 2014-03-19 | 2015-10-08 | 株式会社日立ハイテクノロジーズ | Inspection apparatus |
| CN205616870U (en) * | 2016-02-02 | 2016-10-05 | 北京化工大学 | A mould for evaluating antibacterial material antibacterial property |
-
2018
- 2018-10-25 CN CN201811250005.4A patent/CN109055206A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201309942Y (en) * | 2008-11-21 | 2009-09-16 | 王升 | Comb-shaped medicament susceptibility test scrip |
| JP2015177768A (en) * | 2014-03-19 | 2015-10-08 | 株式会社日立ハイテクノロジーズ | Inspection apparatus |
| CN205616870U (en) * | 2016-02-02 | 2016-10-05 | 北京化工大学 | A mould for evaluating antibacterial material antibacterial property |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113820337A (en) * | 2021-11-24 | 2021-12-21 | 深圳市丰用实业集团有限公司 | Machine vision-based antibacterial ecological plate production and manufacturing quality detection early warning system |
| CN113820337B (en) * | 2021-11-24 | 2022-03-15 | 深圳市丰用实业集团有限公司 | Machine vision-based antibacterial ecological plate production and manufacturing quality detection early warning system |
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Application publication date: 20181221 |
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