KR20220097222A - A composition for enhancing exercise performance and fatigue recovery, containing fermented ripen Noni, Coconut sugar and Muscovado sugar as active ingredients - Google Patents

Abstract

The present invention relates to a composition for enhancing exercise performance and fatigue recovery, containing fermented-aged noni, coconut sugar, or Muscovado sugar as an active ingredient and, more specifically, to a composition for enhancing exercise performance and fatigue recovery, which has the effects of increasing glycogen storage in tissues and a liver function to improve exercise performance and inhibit the accumulation of fatigue substances in blood. The present invention provides a food composition for exercise performance enhancement and fatigue resistance useful for the food industry since glycogen storage in tissues and a liver function increase to improve exercise performance and inhibit the accumulation of fatigue substances in the blood for enhancing the ability to sustain exercise when a complex composition of fermented-aged noni and coconut sugar is ingested. A composition according to the present invention promotes the reduction of fatigue substances to rapidly recover from fatigue or rapidly increase exercise performance such that sports drinks, functional foods, and pharmaceutical composition products effective for fatigue recovery and exercise performance increase can be manufactured.

Description

A composition for enhancing exercise performance and fatigue recovery, containing fermented ripen Noni, Coconut sugar and Muscovado sugar as active ingredients;

The present invention relates to a composition comprising a fermented aged noni, coconut sugar or a fermented moscovado sugar complex composition as an active ingredient, and more particularly, to increase glycogen storage and liver function in tissues to improve exercise performance and reduce blood fatigue It has the effect of inhibiting the accumulation of and relates to a composition for improving exercise performance and fatigue recovery useful in the food industry.

In general, if muscles are not continuously exercised, muscle function deteriorates with aging, and muscle mass and neuromuscular junction (motor unit) decrease. falls sharply

When a signal of fatigue comes, the body needs time to rest and recover, but in today's busy society, it is difficult to properly maintain this cycle of fatigue and fatigue recovery. Accumulation of fatigue due to overwork can lead to chronic fatigue and many diseases such as peptic ulcer, high blood pressure, and diabetes. In addition, cancer, stroke, and heart disease are also the three major causes of death in modern people, and overwork is the main cause of these.

Expressing that fatigue or stress has accumulated means that the functions of the mind and body are not performed smoothly. In the case of fatigue, it can be said that the force required for muscle contraction activity is not sufficiently exerted, that is, the exercise performance is reduced. can be understood as an imbalance. Therefore, fatigue in a broad sense is a concept that includes both fatigue and stress, and can be said to be a decrease in ability to perform physical or mental activities. Fatigue refers to a state in which homeostasis is disrupted.

In order to prevent this, it is recommended that you continue to exercise, such as resistance exercise, along with an appropriate diet. Recently, with the well-being craze, the importance of exercise is being emphasized to enjoy a leisurely life and to prevent and treat various adult diseases. As such, regular exercise is required to improve the quality of life, and there is a tendency that not only athletes but also ordinary people require more energy and endurance in daily life. Therefore, research on supplements, functional foods, and food compositions to improve physical exercise performance has been continued for a long time, and in fact, it is known that taking compounds such as steroids and caffeine increases exercise capacity. However, these drugs have fatal side effects Its use is extremely limited because it can be accompanied by

Research on natural food materials has been actively conducted as it has been found that plants that exist in nature contain a large amount of functional ingredients having bioregulatory functions such as disease prevention and aging suppression. Recently, research to develop functional supplements using natural products with guaranteed safety, such as plant extracts, is being actively conducted.

Accordingly, the present inventors continued their research on fermented and aged noni and coconut sugar, and confirmed that exercise performance was increased and the accumulation of blood fatigue factors was suppressed by ingestion of these complexes, and completed the present invention.

1. Beck L., 2014, “Coconut sugar: Is it healthier than white sugar, or just hype?”, The Globe & Mail, Retrieved 30 May 2015 2. Timothy P. E., 2016 Postprandial insulin and glucose levels are reduced in healthy subjects when a standardized breakfast meal is supplemented with a filtered sugarcane molasses concentrate. Eur. J. Nutr., 55(8), 2365-2376

SUMMARY OF THE INVENTION The present invention aims to solve the above problems and other problems related thereto.

The present inventors have been conducting research on a composition composed of natural ingredients that can effectively promote exercise performance and fatigue recovery, and the composition containing fermented and aged noni, coconut sugar or Moscow sugar has improved exercise performance and fatigue recovery activity. It was confirmed that there is, and the present invention was completed.

One object of the present invention is to provide a composition for improving exercise performance and/or fatigue recovery comprising fermented and aged noni, coconut sugar and Moscow sugar as active ingredients.

Another object of the present invention is to provide a method for preparing a composition comprising the fermented and aged noni, coconut sugar and Moscow sugar.

Another object of the present invention is to provide a food composition for improving exercise performance and / or fatigue recovery.

Another object of the present invention is to provide a pharmaceutical composition for improving exercise performance and/or fatigue recovery.

Another object of the present invention is to provide a quasi-drug composition for improving exercise performance and/or fatigue recovery.

Another object of the present invention is to provide a feed composition for improving exercise performance and/or fatigue recovery.

The technical problem to be achieved according to the technical idea of the invention disclosed in this specification is not limited to the problem for solving the above-mentioned problems, and another problem not mentioned can be clearly understood by those skilled in the art from the description below. There will be.

This will be described in detail as follows. Meanwhile, each description and embodiment disclosed in the present application may be applied to each other description and embodiment. That is, all combinations of the various elements disclosed in the present application fall within the scope of the present application. In addition, it cannot be seen that the scope of the present application is limited by the detailed description described below.

In order to solve the above problems, the present invention provides a composition for improving exercise performance and fatigue recovery using fermented and aged noni, coconut sugar and/or Moscow sugar as an active ingredient.

Hereinafter, the present invention will be described in more detail.

In the present invention, "fermented and aged noni" refers to noni fermented and aged by inoculating noni with lactic acid bacteria. Here, the noni is not limited to the portion thereof, but noni fruit may be used as an example.

In the present invention, "fermented noni" may be a fermented product in which noni fruits are inoculated with any one or more of the following 7 types of lactic acid bacteria:

(1) Lactobacillus plantarum (Lactobacillus plantarum);

(2) Lactobacillus paracasei;

(3) Lactobacillus rhamnosus;

(4) Lactobacillus casei (Lactobacillus casei);

(5) Lactobacillus fermentum (Lactobacillus fermentum);

(6) Lactobacillus reuteri; and

(7) Lactococcus lactis subsp. lactis.

In the present invention, in the "fermented noni", scopolin is scopoletin, and asperuloside is deacetylasperulosidic acid (DAA) and asperurosidic acid ( asperulosidic acid).

In the present invention, the content of bioconverted scopoletin in the "fermented noni" is 100 to 2000 μg/g (based on the solid content of the fermented noni extract 60%), and the content of diacetyl asperurosidic acid is 0.5 to 15 mg/g (based on fermented noni extract solid content of 60%), and the content of asperurosidic acid is 0.2-5 mg/g (based on fermented noni extract solid content of 60%).

In the present invention, the contents of scopoletin, diacetylasperurosidic acid and asperurolsidic acid, which are functional ingredients contained in "fermented noni", are the same even in "fermented and aged noni".

In the present invention, "fermented and aged noni" may be fermented and aged noni itself or an extract obtained therefrom. As an example, the fermented-aged noni of the present invention is fermented and aged by inoculating lactic acid bacteria on the noni fruit, and the liquid obtained by squeezing the fermented-aged noni may be an extract.

In the present specification, "extract" is a meaning commonly used in the art as a crude extract, but in a broad sense also includes the meaning of a fraction obtained by additionally fractionating the extract. That is, the fermented and aged noni extract includes not only those obtained by using the above-mentioned extraction solvent, but also those obtained by additionally applying a purification process thereto. For example, a fraction obtained by passing the extract through an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (prepared for separation according to size, charge, hydrophobicity, or affinity), etc. The fractions obtained through various purification methods are also included in the fermented and aged noni extract of the present invention.

As used herein, "including as an active ingredient" means including an amount sufficient to achieve the efficacy or activity of the fermented aged noni or its extract. In the present invention, as a composition extracted from fermented and aged noni, which is a natural plant material, there is no side effect to the human body even when administered in excess. can

The fermentation-aged Noni extract according to the present invention is extracted using an organic solvent, and the extracts that can be used as the extraction solvent are as follows.

First, suitable as a polar solvent are (i) water, (ii) an alcohol having 1 to 6 carbon atoms (preferably methanol, ethanol, propanol, butanol, n-propanol, iso-propanol, n-butanol, 1-pentane). ol, 2-butoxyethanol or ethylene glycol), (iii) acetic acid, (iv) DMFO (dimethyl-formamide), and (v) DMSO (dimethyl sulfoxide).

And suitable as a non-polar solvent are acetone, acetonitrile, ethyl acetate, methyl acetate, fluoroalkane, pentane, hexane, 2,2,4-trimethylpentane, decane, cyclohexane, cyclopentane, diisobutylene, 1 -pentene, 1-chlorobutane, 1-chloropentane, o-xylene, diisopropyl ether, 2-chloropropane, toluene, 1-chloropropane, chlorobenzene, benzene, diethyl ether, diethyl sulfide, chloroform, dichloromethane, 1,2-dichloroethane, aniline, diethylamine, ether, carbon tetrachloride, methylene chloride, petroleum ether and THF.

As a solvent that can be used as an example, (a) water, (b) anhydrous or lower alcohols having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol, etc.), (c) a mixed solvent of the lower alcohol and water, (d) ) acetone, (e) ethyl acetate, (f) chloroform, (g) butyl acetate, (h) 1,3-butylene glycol, (i) hexane and (j) diethyl ether are preferably used, For easy extraction, extraction may be performed using water, ethanol, or a mixture of water and ethanol.

In addition, the fermentation-aged Noni extract used in the present invention may be prepared in a powder state by additional processes such as distillation under reduced pressure and freeze-drying or spray-drying.

In the present invention, the fermentation-aged Noni and coconut sugar may be in a weight ratio of 70: 30 to 95: 5, more preferably 80: 20 to 90: 10 by weight.

In addition, the fermentation-aged Noni and Moscovado sugar may be in a weight ratio of 70: 30 to 95: 5, more preferably 80: 20 to 90: 10 by weight.

In addition, the fermentation and aging noni, coconut sugar and Moscow sugar may be characterized in that 90: 5: 5 to 70: 15: 15 weight ratio, more preferably 90: 5: 5.

According to one aspect of the present invention, the present invention relates to a food composition for improving exercise performance or fatigue recovery comprising fermented and aged noni, coconut sugar and/or Moscow sugar.

The food composition may include a health functional food composition, and the health functional food composition includes various foods, beverages, gum, tea, vitamin complexes, health supplements, and the like, and powders, granules, pills, tablets, and capsules. , candy, syrup or beverage. In addition to the active ingredient, the food composition of each dosage form can be appropriately selected by those skilled in the art without difficulty depending on the dosage form or purpose of use in addition to the active ingredient, and a synergistic effect may occur when applied simultaneously with other raw materials.

The composition may contain other ingredients capable of giving a synergistic effect to the main effect within a range that does not impair the main effect aimed at the present invention. For example, in order to improve physical properties, additives such as fragrances, pigments, disinfectants, antioxidants, preservatives, humectants, thickeners, inorganic salts, emulsifiers, and synthetic polymers may be further included.

When the health functional food composition of the present invention is used as a food additive, the fermented and aged noni, coconut sugar, and Moscow sugar may be added as it is, or it may be used together with other foods or food ingredients, and may be appropriately used according to a conventional method. have. The mixing amount of the active ingredient may be appropriately determined according to the purpose of its use (prevention, health or therapeutic treatment). There is no particular limitation on the type of the food. Examples of foods to which fermented and aged noni, coconut sugar, and Moscow sugar can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, There are various soups, beverages, teas, drinks, alcoholic beverages, vitamin complexes, etc., and includes all health functional foods in the ordinary sense.

When the composition of the present invention is used as a health drink, it may contain various flavoring agents or natural carbohydrates as an additional component like a conventional drink. The above-mentioned natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclotenstrin, and sugar alcohols such as xylitol, sorbitol and erythritol. As the sweetener, natural sweeteners such as taumarin and stevia extract, synthetic sweeteners such as saccharin or aspartame, and the like can be used.

The present invention also provides a method for preparing a composition comprising the fermentation-aged noni, coconut sugar and/or Moscow sugar.

According to one aspect of the present invention, the present invention comprises the steps of: obtaining a fermented aged noni obtained by fermenting and/or aging Noni using lactic acid bacteria; And it provides a method for producing a composition comprising fermented aged noni and coconut sugar and/or Moscow sugar, comprising the step of aging by adding coconut sugar and/or Moscow sugar to the fermented aged noni.

In the present invention, the method comprises the steps of: (a) inoculating lactic acid bacteria in noni fruit to ferment and ripen to produce fermented aged noni; (b) juicing the fermented and aged noni; And (c) it provides a method for producing a composition comprising fermented-aged Noni, coconut sugar and/or Moscow sugar, comprising the step of adding and mixing coconut sugar and/or Moscow sugar to the fermented-aged Noni.

In the present invention, the manufacturing method may further include the step of (d) mixing fermented and aged noni with coconut sugar and/or Moscow sugar in step (c) and then further aging.

In the present invention, the fermentation and aging, but is not limited thereto, may be made for 48 to 168 hours at 35 to 40 ℃.

The aging may be carried out by pouring the fermentation broth produced in the fermentation process on top of the fermented product every 1 to 3 days, more specifically, every 2 days.

In the present invention, sugar obtained by boiling the sap of palm trees belonging to the palm family, such as coconut (coco palm), palmyra palm, date palm, nipa palm, and sugar palm, is called palm sugar or palm sugar. , Coconut sugar (also called coconut sugar) refers to palm sugar produced from the sap of the bud stem of the coconut palm, and is a commercially available substance.

The main components of coconut sugar are 70-79% sucrose and 3-9% each of glucose and fructose (Beck L., 2014, "Coconut sugar: Is it healthier than white sugar, or just hype?", The Globe & Mail, Retrieved 30 May 2015).

Muscovado sugar is unrefined sugar obtained by boiling sugarcane juice (juice) to form molasses. It is a sugar manufactured by drying and pulverizing dark brown granules. Phosphoric acid, formic acid, sulfur dioxide, preservatives, or tablets It refers to sugar that is completely free from chemicals such as bleach or viscosity modifiers used in refined sugar. It has a higher molasses content than processed white or brown sugar, and has a stronger and darker flavor. It is a commercially available material.

According to one aspect of the present invention, the present invention relates to a pharmaceutical composition for improving exercise performance or fatigue recovery comprising fermented and aged noni, coconut sugar and/or Moscow sugar.

The pharmaceutical composition according to the present invention may contain an active ingredient alone or may be formulated in a suitable form together with a pharmaceutically acceptable carrier, and may further contain an excipient or diluent. As used herein, 'pharmaceutically acceptable' refers to a non-toxic composition that is physiologically acceptable and does not normally cause allergic reactions or similar reactions such as gastrointestinal disorders and dizziness when administered to humans.

The pharmaceutically acceptable carrier may further include, for example, a carrier for oral administration or a carrier for parenteral administration. Carriers for oral administration may include lactose, starch, cellulose derivatives, magnesium stearate, stearic acid, and the like. In addition, various drug delivery materials used for oral administration of the peptide formulation may be included. In addition, the carrier for parenteral administration may include water, a suitable oil, saline, aqueous glucose and glycol, and the like, and may further include a stabilizer and a preservative. Suitable stabilizers include antioxidants such as sodium hydrogen sulfite, sodium sulfite or ascorbic acid. Suitable preservatives include benzalkonium chloride, methyl- or propyl-paraben and chlorobutanol. The pharmaceutical composition of the present invention may further include a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, etc. in addition to the above components. For other pharmaceutically acceptable carriers and agents, reference may be made to those described in the following documents.

The composition of the present invention may be administered to mammals including humans by any method. For example, it can be administered orally or parenterally. Parenteral administration methods include, but are not limited to, intravenous, intramuscular, intraarterial, intramedullary, intrathecal, intracardiac, transdermal, subcutaneous, intraperitoneal, intranasal, enteral, topical, sublingual or rectal administration. can be

The pharmaceutical composition of the present invention may be formulated as a formulation for oral administration or parenteral administration according to the administration route as described above.

In the case of a formulation for oral administration, the composition of the present invention may be formulated as a powder, granule, tablet, pill, dragee, capsule, liquid, gel, syrup, slurry, suspension, etc. using methods known in the art. can For example, oral preparations can be obtained by mixing the active ingredient with a solid excipient, grinding it, adding suitable adjuvants, and processing it into a granule mixture to obtain tablets or dragees. Examples of suitable excipients include sugars, including lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol and maltitol, and starches, including corn starch, wheat starch, rice starch and potato starch, cellulose, Cellulose, including methyl cellulose, sodium carboxymethylcellulose and hydroxypropylmethyl-cellulose, and the like, fillers such as gelatin, polyvinylpyrrolidone, and the like may be included. In addition, cross-linked polyvinylpyrrolidone, agar, alginic acid or sodium alginate may be added as a disintegrant if necessary. Furthermore, the pharmaceutical composition of the present invention may further include an anti-aggregating agent, a lubricant, a wetting agent, a flavoring agent, an emulsifying agent, and an antiseptic agent.

Formulations for parenteral administration may be formulated in the form of injections, creams, lotions, external ointments, oils, moisturizers, gels, aerosols and nasal inhalants by methods known in the art. These formulations are described in Remington's Pharmaceutical Science, 19th ed., Mack Publishing Company, Easton, PA, 1995, which is a commonly known recipe for all pharmaceutical chemistry.

The total effective amount of the composition of the present invention may be administered to a patient as a single dose, or may be administered by a fractionated treatment protocol in which multiple doses are administered for a long period of time. The pharmaceutical composition of the present invention may vary the content of the active ingredient depending on the severity of the disease. Preferably, the preferred total dose of the pharmaceutical composition of the present invention may be about 0.01 μg to 10,000 mg, preferably 0.1 μg to 500 mg, and most preferably 100 mg to 500 mg per kg of patient body weight per day. However, the dosage of the pharmaceutical composition is determined by considering various factors such as the formulation method, administration route and number of treatments, as well as the patient's age, weight, health status, sex, severity of disease, diet and excretion rate, etc., the effective dosage for the patient is determined. Therefore, in consideration of this point, those of ordinary skill in the art will be able to determine an appropriate effective dosage of the composition of the present invention. The pharmaceutical composition according to the present invention is not particularly limited in its formulation, administration route and administration method as long as the effect of the present invention is exhibited.

According to one aspect of the present invention, the present invention relates to a quasi-drug composition for improving exercise performance or fatigue recovery comprising fermented and aged noni, coconut sugar and/or Moscow sugar.

The term "quasi-drug" as used in the present invention refers to articles that are not instruments, machines, or devices, among articles used for the purpose of diagnosing, treating, alleviating, treating, or preventing diseases of humans or animals, and those that are not pharmacologically related to the structure and function of humans or animals. It refers to articles other than instruments, machines, or devices used for the purpose of influencing, and may include, but is not limited to, preparations for internal use in one embodiment, the formulation method, dose, and method of use of quasi-drugs , components and the like may be appropriately selected from conventional techniques known in the art.

The quasi-drug composition of the present invention may further include a pharmaceutically acceptable carrier, excipient or diluent if necessary in addition to the above components. The pharmaceutically acceptable carrier, excipient or diluent is not limited as long as it does not impair the effects of the present invention, and includes, for example, fillers, extenders, binders, wetting agents, disintegrants, surfactants, lubricants, sweeteners, fragrances, preservatives, etc. may include

According to one aspect of the present invention, the present invention relates to a feed composition for improving exercise performance or fatigue recovery comprising fermented and aged noni, coconut sugar and/or Moscow sugar.

In the present invention, the feed composition may be formulated in a conventional feed form, and may include known feed ingredients together. In addition, it can be used as a feed additive, which is added in the form of an additive to the feed used. The feed additive of the present invention corresponds to an auxiliary feed under the Feed Management Act, and is a mineral preparation such as sodium bicarbonate (bicarbonate), bentonite, magnesium oxide, and composite minerals, and trace minerals such as zinc, copper, cobalt, and selenium. Preparations, kerotene, vitamin E, vitamins A, D, E, nicotinic acid, vitamins such as vitamin B complex, protective amino acids such as methionine and lyic acid, protective fatty acids such as fatty acid calcium salt, probiotics (lactic acid bacteria), yeast culture Water, live bacteria such as mold fermented products, yeast agents, and the like may be further included.

The feed or feed additive of the present invention can be applied to a number of animal diets including mammals, poultry and fish.

The fermented aged noni and coconut sugar or moscovado sugar complex composition included in the complex composition for improving exercise performance and fatigue recovery according to the present invention has the effect of improving exercise performance and fatigue recovery. In addition, the fermented and aged noni has an effect that can improve digestion and absorption is secreted in the process of fermenting the noni fruit, thereby promoting digestion and defecation activity in adults and the elderly with relatively low digestive power, while being a natural raw material. It has no toxicity or side effects, so it is safe to consume.

In addition, the process of manufacturing the fermented and aged noni can greatly reduce the manufacturing cost, thereby greatly reducing the purchase cost burden of consumers.

In addition, the fermented and aged noni and coconut sugar or Moscow sugar complex composition has improved taste and sensuality compared to the existing noni, so that consumers can enjoy it without burden.

1 is a graph showing the results of measuring the exercise performance time after ingesting a control, a single group and a composite composition of the present invention, and then forced swimming.
2 is a graph showing the results of measuring the concentration of lactic acid in the blood, which changes after ingesting a control, a single group, and a complex composition of the present invention, and then forced swimming.
3 is a graph showing the results of measuring inorganic phosphate after ingesting a control, a single group and a complex composition of the present invention, and then forced swimming.
4 is a graph showing the results of measuring the Creatine kinase concentration after ingesting the control group, the single group, and the composite composition of the present invention, and then forced swimming.
Figure 5 shows the difference between the crystals and the sensory (form) of unrefined sugar and Moscovado sugar.

Hereinafter, the present invention will be described in more detail through the following examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples only.

The composition for improving exercise performance and fatigue recovery was prepared through the following process.

Example 1: Preparation of the composition for improving exercise performance and fatigue recovery of the present invention

1.1: fermented and aged noni production

690 kg of noni fruits were inoculated with complex lactic acid bacteria (7 types of complex lactic acid bacteria AON1805, Lactomason Co., Ltd.) and fermented at 37°C for 45 days or more. Fermentation and maturation were carried out while receiving 10 L of the fermentation broth every 7 days from the start date of fermentation and transferring it to the top of the noni fruit. At this time, the composition of the complex lactic acid bacteria used (7 types of complex lactic acid bacteria AON1805, Lactomason Co., Ltd.) is shown in Table 1 below.

After the aging was completed, the fermented and aged noni was extracted to remove solids, and a fermented and aged noni extract was obtained.

1.2: Coconut Sugar Manufacturing

After extracting the juice from the flower stalk of a coconut tree, heating it at 90°C in a traditional way and stirring, the coconut flower stalk juice is concentrated until it becomes blue, and then hardened. did

1.3: Moscow (Muscovado) sugar production

Remove the leaves and impurities of the harvested sugar cane, put the juice extracted from the sugar cane in a large pot, boil it for a sufficient time, remove the impurities and sediment that floated to the top, and when the moisture evaporates, turn it over until it becomes a powder. It was dried while giving and then finely pulverized to prepare muscova sugar.

1.4: Composite manufacturing using fermented and aged noni extract and coconut sugar

The fermented and aged noni extract and coconut sugar prepared in 1.1 to 1.3 were mixed at different mixing ratios, and then aged for 15 days to prepare a complex.

[Composite Manufacturing]

Complex 1: Fermented and aged noni extract (wt.%) 90: Coconut sugar (wt.%) 10

Complex 2: Fermented and aged noni extract (wt.%) 80: Coconut sugar (wt.%) 20

Complex 3: Fermented and aged noni extract (wt.%) 70: Coconut sugar (wt.%) 30

1.5: Composite manufacturing using fermented and aged noni extract and moscovado sugar

A complex was prepared by mixing the fermentation-aged Noni extract prepared in 1.1 to 1.3 and Moscovado sugar at different mixing ratios and then aging for 15 days.

[Composite Manufacturing]

Complex 4: Fermented and aged noni extract (wt.%) 90: Moscovado sugar (wt.%) 10

Complex 5: Fermented and aged noni extract (wt.%) 80: Moscovado sugar (wt.%) 20

Complex 6: Fermented and aged noni extract (wt.%) 70: Moscovado sugar (wt.%) 30

1.6: Composite manufacturing using fermented and aged noni extract, coconut sugar and Moscow sugar

The fermented and aged noni extract prepared in 1.1 to 1.3, coconut sugar, and Moscow sugar were mixed at different mixing ratios, and then aged for 15 days to prepare a complex.

[Composite Manufacturing]

Complex 7: Fermented and aged noni extract (% by weight) 90: Coconut sugar (% by weight) 5: Moscow sugar (% by weight) 5

Complex 8: Fermented and aged noni extract (wt.%) 80: Coconut sugar (wt.%) 10: Moscovado sugar (wt.%) 10

Complex 9: Fermented and aged noni extract (% by weight) 70: Coconut sugar (% by weight) 15: Moscow sugar (% by weight) 15

1.7: Composite manufacturing using fermented and aged noni extract and sugar

The fermented and aged noni extract prepared in 1.1 and the unrefined sugar were mixed at different mixing ratios, and then aged for 15 days to prepare a complex.

[Composite Manufacturing]

Complex 10: Fermented and aged noni extract (wt.%) 90: unrefined sugar (wt.%) 10

Experimental Example 1: Exercise performance enhancement test

The single extracts prepared in Examples 1.1 to 1.3 and the complexes prepared in 1.4 to 1.6 confirmed the effect of enhancing exercise performance.

(1) Induction of mouse fatigue

After receiving the ICR mouse (Orient Bio, Korea) weighing 30 g, and adapting the light/dark cycle to 12 hours (light/dark cycle) for 1 week in a temperature condition of 20°C to 24°C and a humidity condition of 55%, the experimental animals 8 each, the experimental groups were classified as follows. Experimental group 1 (non-exercise group, hereinafter indicated as Normal), Experimental group 2 (exercise control group, hereinafter indicated as Control), Experimental group 3 (fermented and aged noni 100mg/kg administration group, hereinafter indicated FN), Experimental group 4 (coconut sugar 100mg/kg administration group, hereinafter CS) Indicated), Experimental group 5 (Moscovado sugar 100mg/kg administered group, hereinafter indicated as MS), Experimental group 6 (Composite 10 administered 100mg/kg, hereinafter indicated as FNS) Experimental group 7 (Composite 1 100mg/kg administered group, hereinafter indicated as C 1), Experimental group 8 (Complex 2 100mg/kg administration group, hereinafter labeled C 2), Experimental group 9 (Composite 3 100mg/kg administration group, hereinafter labeled C 3), Experimental group 10 (Composite 4 100mg/kg administration group, hereinafter labeled C 4), Experimental group 11 ( Composite 5 100mg/kg administration group, hereinafter labeled C 5), Experimental group 12 (Composite 6 100mg/kg administration group, hereinafter labeled C 6), Experimental group 13 (Composite 7 100mg/kg administration group, hereinafter labeled C 7), Experimental group 14 (Composite 8 100mg/kg administration group, below C 8), and experimental group 15 (Composite 9 100mg/kg administration group, below C 9) were set, and the test substance was administered once/day for a total of 4 weeks.

(2) Measurement of exercise performance of complexes

After maintaining the breeding conditions in a stable state from 16 hours before the start of the experiment, about 70% of water was put in an acrylic plastic tank (70cm×70cm×60cm), and the animals were allowed to swim in the tank. The swimming time was set as the end point when the experimental animal was exhausted and no longer moving, and the time from the start time to the end time was measured as the swimming time.

As a result, as shown in FIG. 1 , the swimming time was measured until exhaustion to measure exercise performance. As a result of the experiment, in all groups treated with the composition of the present invention based on the control group under the same concentration conditions, the swimming time increased compared to the single group (fermented noni, coconut sugar, and Moscow sugar administered group), so the composition intake was more exercise than the single group intake. It was confirmed that performance was improved, and in particular, in the group administered with Complex 1, 4, and 7, the swimming time was the highest at 1081 to 1119 seconds, confirming that the exercise performance was excellent.

On the other hand, the swimming time of the FNS group, which is a complex of fermented and aged noni extract and unrefined sugar, was 913, which was significantly lower than the group administered with complexes 1, 4, and 7.

These results showed that the improvement of exercise performance by the coconut sugar, Moscowdo sugar, and fermented and aged noni complex was better than the increase in exercise performance by sugar supply.

The composition of the present invention may increase the movement distance and exercise time, as a result of the behavioral experiment, and may exhibit an increase in exercise capacity. In addition, the composition may increase the concentration of blood glucose, increase the concentration of glycogen in the muscle, and decrease the concentration of lactate dehydrogenase (LDH).

Experimental Example 2: Fatigue recovery enhancement test

(1) Determination of lactic acid content

According to Experimental Example 1, a forced swimming test was performed on the rats raised on the day of the end of the administration after the experiment to measure the production of lactic acid in the blood, which is a typical fatigue substance.

After maintaining the breeding conditions in a stable state from 16 hours before the start of the experiment, about 70% of water was placed in an acrylic plastic tank (70cmХ70cmХ60cm) and allowed to swim in the water tank. The swimming time was 15 minutes and the same was applied to all experimental animals. After swimming, to measure muscle fatigue after aerobic exercise, serum lactate content was measured using a lactate meter (Lactate Pro LT-1710, ARKRAY Inc. / JAPAN) by collecting blood from the tail every 10 minutes during the rest period.

As a result, immediately after swimming, the concentration of lactic acid increased by more than 6.5 times compared to the stabilization period, and the composition group significantly increased the blood lactic acid content under the same concentration conditions compared to the control group, fermented and aged noni, coconut sugar, and Moscow sugar alone group. has been shown to decrease to In particular, under the same concentration conditions, the test group of the composition of the present invention showed a 4% to 55% lower lactic acid concentration than the control group, thereby remarkably reducing lactic acid, a substance that causes muscle fatigue.

In particular, the lactic acid concentration of the FNS group, which is a complex of fermented and aged noni extract and unrefined sugar, was 4.34, which was significantly higher than that of the groups administered with complex 1 (C 1), 4 (C 4), and 7 (C 7). became

Accordingly, it was confirmed that the composition of the present invention promotes recovery from fatigue, and thus has excellent efficacy for improving exercise capacity.

(2) Blood chemistry test after swimming fatigue

After the forced swimming test was conducted on the mice raised on the day of the end of the administration after the experiment according to Experimental Example 1, the blood was collected from the heart after sacrifice, and the separated serum was requested to the Green Cross Research Institute, and the blood inorganic phosphorus (inorganic phosphorus) ) concentration and creatine kinase (CK) concentration were measured.

During exercise, ATP is hydrolyzed at the stage of increasing the affinity between myosin and actin by repeated muscle contractions in the muscle, which leads to a sharp increase in the concentration of inorganic phosphate in the blood. It is known that when the serum inorganic phosphate concentration rapidly increases during exercise, the cross-bridge of muscle fibers is weakened and force generation is reduced. Accordingly, the concentration of inorganic phosphate in the blood is used as an important indicator for muscle fatigue.

As a result, the concentration of inorganic phosphate in the serum was significantly decreased in the complex administration group compared to the exercise control group. Complex 1, 4, and 7 administration groups showed the lowest values at 11.01 mg/dL, 11.18 mg/dL, and 11.07 mg/dL, respectively, confirming that it was effective in recovering from fatigue by reducing the content of inorganic phosphate, a factor in fatigue.

However, the complex of fermented and aged noni extract and seoltyeun (FNS) also showed a significant decrease in the exercise control group at a concentration of 12.58 mg/dL, but showed a similar level of inorganic phosphate to that of the single administration group of fermented and aged noni.

On the other hand, it was irradiated with a high level of inorganic phosphoric acid concentration when compared to the groups administered with Complex 1, 4 and 7.

Creatine kinase enzyme is EC 2.7. 3.2. An enzyme discovered in skeletal muscle by K. Lohmans in 1943, it is an enzyme that catalyzes the reaction of creatine and ATP to produce creatine phosphate. The Roman reaction of phosphocreatine + ADP creatine + ATP (a high-energy phosphate group from phosphocreatine to ADP catalyzes a reaction that converts to ATP and synthesizes ATP). When a large amount of ATP is consumed during contraction of skeletal muscle, which is abundant in muscle, ATP obtained from phosphocreatine, which is present in large amount in the muscle, is generated and supplied.

As a result of the test, the creatine kinase enzyme of the exercise control group was irradiated with a level of 562 IU/L, and both the single group and the complex administration group were irradiated with higher levels than the exercise control group.

In particular, the complex 1 (C 1), complex 4 (C 4), and complex 7 (C 7) administration groups showed the highest values at 967 IU/L, 955 IU/L and 966 IU/L, respectively, under the same concentration condition. It was confirmed that it was effective in alleviating muscle fatigue by promoting ATP resynthesis.

In the case of fermented aged noni and unrefined sugar complex FNS administered with added sugar, as in Complex 1, 4, and 7 administration groups, the concentration of creatine kinase enzyme (709 IU.L) was significantly lower.

From the above description, those skilled in the art to which the present invention pertains will understand that the present invention may be embodied in other specific forms without changing the technical spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention, rather than the above detailed description, all changes or modifications derived from the meaning and scope of the claims to be described later and their equivalents.

Claims (18)

Fermented and aged noni or its extract; coconut sugar; Or a food composition for improving exercise performance or fatigue recovery comprising Moscow sugar as an active ingredient.
According to claim 1,
Fermented and aged noni or its extract; coconut sugar; And a food composition for improving exercise performance or fatigue recovery comprising a combination of two or more of Moscow sugar as an active ingredient.
3. The method of claim 2,
The fermented and aged noni or its extract; And coconut sugar is 90: 10 to 70: a weight ratio of 30, a food composition for promoting exercise performance or fatigue recovery.
3. The method of claim 2,
The fermented and aged noni or its extract; And Moscow sugar is 90: 10 to 70: 30 weight ratio, a food composition for promoting exercise performance or fatigue recovery.
3. The method of claim 2,
The fermented and aged noni or its extract; coconut sugar; And Moscow sugar is 90: 5: 5 to 70: 15: 15 weight ratio, a food composition for promoting exercise performance or fatigue recovery.
According to claim 1,
The fermented aged noni or its extract is fermented and aged noni obtained by fermenting and aging noni using lactic acid bacteria, a food composition for improving exercise performance or fatigue recovery.
According to claim 1,
The enhancement of the exercise performance includes muscle strength strengthening, physical strength enhancement, muscle fatigue delay, mitochondrial activity enhancement, balance enhancement, endurance enhancement, prevention of muscle damage or suppression of muscle atrophy, a food composition for promoting exercise performance or fatigue recovery .
8. The method according to any one of claims 1 to 7,
The food composition is a health functional food, a food composition for improving exercise performance or fatigue recovery.
According to claim 1,
The food composition is a powder, granules, pills, tablets, capsules, candy, syrup, and any one formulation selected from beverages, a food composition for improving exercise performance or fatigue recovery.
Fermented and aged noni or its extract; coconut sugar; Or a pharmaceutical composition for improving exercise performance or fatigue recovery comprising Moscow sugar as an active ingredient.
Fermented and aged noni or its extract; coconut sugar; Or a quasi-drug composition for improving exercise performance or fatigue recovery comprising Moscow sugar as an active ingredient.
Fermented and aged noni or its extract; coconut sugar; Or a feed composition for improving exercise performance or fatigue recovery comprising Moscow sugar as an active ingredient.
(a) obtaining a fermented aged noni obtained by fermenting and aging Noni using lactic acid bacteria; and (b) adding coconut sugar to the fermented and aged noni for aging.
(a) obtaining a fermented aged noni obtained by fermenting and aging Noni using lactic acid bacteria; And (b) a method for producing a composition comprising fermented-aged noni or its extract and moscovado sugar, comprising the step of adding and aging the fermented aged noni with Moscow sugar.
(a) obtaining a fermented aged noni obtained by fermenting and aging Noni using lactic acid bacteria; and (b) adding coconut sugar and Moscow sugar to the fermented and aged noni and aging them.
16. The method according to any one of claims 13 to 15,
After step (a), the method further comprising the step of squeezing the fermented aged noni obtained in step (a) to obtain a fermented aged noni extract.
16. A composition obtained according to any one of claims 13 to 15.
18. The method of claim 17,
The composition is a composition having the ability to improve exercise performance or fatigue recovery.

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