KR20220094453A - Alginate gel complex for removing microcalcium stones and method for preparing thereof - Google Patents

Abstract

The present invention relates to an alginate gel complex for removing stones and a preparation method thereof, and more specifically, to an alginate gel complex for removing stones which completely removes stones consisting of calcium, oxalic acid, and uric acid as main components without remaining stones and has a biological and antibacterial effect to prevent urinary tract infection after surgery and a preparation method thereof.

Description

Alginate gel complex for removing stones and method for preparing the same {Alginate gel complex for removing microcalcium stones and method for preparing thereof}

The present invention relates to an alginic acid gel complex for removing stones and a method for manufacturing the same, and more specifically, a living body capable of completely removing stones composed of calcium, oxalic acid, uric acid, etc. as main components without residual stones and preventing urinary tract infection after surgery It relates to an alginic acid gel complex for removing stones that are friendly and exhibit an antibacterial effect at the same time, and a method for preparing the same.

Urinary tract obstruction is a common disease in the urology department, accounting for more than 25% of patients visiting the urology department as a symptom of an advanced country that is steadily increasing due to the improvement of living standards and changes in diet.

The main components of urinary stones are calcium, oxalic acid, phosphoric acid, and uric acid, and rarely magnesium ammonium phosphate, cystine, and the like. Urinary stones can be easily formed when these components are excessively excreted in the urine, and factors that promote their crystallization and crystallization include urine acidity, urinary tract infection, urinary tract congestion, and the like. When urolithiasis occurs, the most common symptoms are colic and hematuria. Colic is caused by excessive peristalsis of the urinary tract muscles due to sudden blockage of the urinary tract, renal capsule, and renal pelvis. Such pain appears in the back, flanks, and abdomen, and the severity is so severe that the pain of the patient cannot be expressed in words. The pain has a continuous or intermittent pattern and is accompanied by nausea, vomiting, and hematuria, and may cause other urination disorders or dysuria.

In general, stones are classified into kidney stones, ureter stones, bladder stones, etc. depending on the site of formation, and the most commonly used methods for these treatments include extracorporeal shock wave lithotripsy, a non-invasive stone crushing method, and a 1-2 cm skin incision. Percutaneous lithotripsy, which removes stones by inserting an endoscope through an endoscope, and methods for removing stones by laparotomy, etc. are being used.

However, it is almost impossible to completely remove even the small remnants that have been shredded even with surgical methods. In addition, due to the remaining stones, there may be problems that stones may recur in the future, and complications due to urinary tract infection may occur due to the spread of bacteria due to the obstruction of the stones.

Therefore, in order to supplement the above problems, the present inventors recognized that it is urgent to develop a method for removing stones that can completely remove stones without residual stones and prevent complications due to urinary tract infection after surgery, and completed the present invention.

Republic of Korea Patent Publication No. 10-1994310 Republic of Korea Patent Publication No. 10-14188090

An object of the present invention is to completely remove stones composed of calcium, oxalic acid, uric acid, etc. as main components without residual stones, and to remove stones that are biocompatible and have antibacterial effects that can prevent urinary tract infection after surgery and alginate gel complex and its To provide a manufacturing method.

The technical problems to be achieved by the invention are not limited to the technical problems mentioned above, and other technical problems not mentioned can be clearly understood by those of ordinary skill in the art from the description of the present invention.

In order to achieve the above object, the present invention provides an alginic acid gel complex for removing stones and a method for preparing the same.

Hereinafter, the present specification will be described in more detail.

The present invention is a cationic cross-linked polymer alginic acid salt (alginate); and a metal salt having antibacterial properties; provides an alginic acid gel complex comprising a.

In the present invention, the alginic acid gel complex is cross-linked with stones containing a metal that is a divalent or trivalent cation to remove the stones.

In the present invention, the alginic acid salt is characterized in that 0.5 × 10 ^-2 M to 5.0 × 10 ^-2 M sodium alginate (sodium alginate) having a concentration.

In the present invention, the metal salt is at least one selected from the group consisting of silver (silver, Ag), copper (copper, Cu), zinc (zinc, Zn), platinum (Pt) and iron (ion, Fe) It is characterized in that it is salt.

In addition, the present invention is a cationic cross-linked polymer alginic acid salt (alginate); And it provides a method for preparing an alginic acid gel complex by mixing a metal salt having antibacterial properties.

All the matters mentioned in the alginic acid gel complex for removing the stones and the preparation method thereof apply equally unless contradictory.

The alginic acid gel complex for removing stones of the present invention and its manufacturing method completely remove stones composed of calcium, oxalic acid, uric acid, etc. can indicate

Effects of the present invention are not limited to the effects mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the description of the claims.

1 is an image confirming the formation of aggregates between the alginate gel complex (Example 1) and calcium oxalate according to the present invention.
2 is an image confirming the formation of aggregates between (a) an actual urolithiasis patient's stones and (b) an alginate gel complex according to the present invention (Example 1).
3 is (a) a control in which only Escherichia coli not containing an alginate gel complex according to the present invention exists, Escherichia coli in (b) Examples 1, (c) Examples 2 and (d) Example 3 This is an image confirming the antibacterial effect of the prepared alginic acid gel complex.
4 is (a) a control in which only Staphylococcus aureus , which does not contain an alginate gel complex according to the present invention, is present, (b) Examples 1, (c) Examples 2 and (d) Example 3 in which Staphylococcus aureus is contacted; This is an image confirming the antibacterial effect of the prepared alginic acid gel complex.

The terms used in this specification have been selected as currently widely used general terms as possible while considering the functions in the present invention, but these may vary depending on the intention or precedent of a person skilled in the art, the emergence of new technology, and the like. In addition, in a specific case, there is a term arbitrarily selected by the applicant, and in this case, the meaning will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, rather than the name of a simple term.

Unless defined otherwise, all terms used herein, including technical and scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning in the context of the related art, and should not be interpreted in an ideal or excessively formal meaning unless explicitly defined in the present application. does not

Numerical ranges are inclusive of the values defined in that range. Every maximum numerical limitation given throughout this specification includes all lower numerical limitations as if the lower numerical limitation were expressly written. Every minimum numerical limitation given throughout this specification includes all higher numerical limitations as if the higher numerical limitation were expressly written. Any numerical limitation given throughout this specification shall include all numerical ranges within the broader numerical range, as if the narrower numerical limitation were expressly written.

Hereinafter, examples of the present invention will be described in detail, but it is obvious that the present invention is not limited by the following examples.

Alginate Gel Complex

The present invention is a cationic cross-linked polymer alginic acid salt (alginate); and a metal salt having antibacterial properties; provides an alginic acid gel complex comprising.

The alginic acid is a complex polysaccharide acid represented by the following [Formula 1] consisting of mannuronic acid and glucuronic acid constituting the cell wall of brown algae. The alginic acid may have a polymerization degree of 80 and a molecular weight of 20,000 to 240,000.

[Formula 1]

The alginic acid salt may be sodium alginate having a concentration of 0.5 × 10 ^-2 M to 5.0 × 10 ^-2 M, preferably 0.5 × 10 ^-2 M to 4.0 × 10 ^-2 M alginic acid having a concentration It may be sodium (sodium alginate), and most preferably, it may be sodium alginate (sodium alginate) having a concentration of 1.0 × 10 ^-2 M to 3.5 × 10 ^-2 M.

The metal salt may be at least one salt selected from the group consisting of silver (Ag), copper (copper, Cu), zinc (zinc, Zn), platinum (Pt), and iron (ion, Fe). More specifically, the silver salt is silver nitrate (AgNO ₃ ), silver nitrite (AgNO ₂ ), silver acetate (CH ₃ COOAg), silver perchlorate (AgClO ₄ ), and silver chlorate ( silver chlorate, AgClO ₃ ) may be at least one selected from the group consisting of, and the copper salt is copper nitrate (Cu(NO ₃ ) ₂ ), copper chloride (CuCl ₂ ), copper sulfate (copper sulfate, CuSO ₄ ), copper acetate, (CH ₃ COO) ₂ Cu), copper acetylacetonate (Cu(O ₂ C ₅ H ₇ ) ₂ ), copper carbonate (CuCO ₃ ), cyclohexane Copper cyclohexane butyrate, (C ₆ H ₁₁ (CH ₂ ) ₃ CO ₂ ) ₂ Cu), copper stearate, (C ₁₈ H ₃₅ O ₂ ) ₂ Cu), copper perchlorate (Cu) (ClO ₄ ) ₂ ) and copper hydroxide (copper(II) hydroxide, Cu(OH) ₂ ) may be at least one selected from the group consisting of, and the zinc salt is zinc chloride (ZnCl ₂ ), zinc sulfate ( zinc sulfate, ZnSO ₄ ), zinc nitrate (zinc nitrate, Zn(NO ₃ ) ₂ ), zinc acetate (zinc acetate, Zn(CH ₃ CO ₂ ) ₂ ), zinc phosphate, Zn ₃ (PO ₄ ) ₂ ) (H ₂ O) ₄ ), zinc fluoride (Zinc fluoride, ZnF ₂ ), zinc bromide (ZnBr ₂ ) and zinc iodide (zinc iodide, ZnI ₂ ) may be at least one selected from the group consisting of, The iron salt is iron nitrate (iron(III) nitrate, Fe(NO ₃ ) ₃ ), iron chloride (iron chloride, FeCl ₂ or FeCl ₃ ), iron hydroxide (iron(III) oxide-hydroxide, Fe(OH) ₃ ) and sulfuric acid It may be at least one selected from the group consisting of iron (iron(II) sulfate, FeSO ₄ ), and the platinum salt is sodium tetrachloroplatinate (II), Na ₂ PtCl ₄ ), potassium tetrachloroplatinate ( potassium tetrachloroplatinate, K ₂ PtCl ₄ ), sodium hexachloroplatinate (sodium hexachloroplatinate(IV), Na ₂ PtCl ₆ ), potassium hexachloroplatinate (sodium hexachloroplatinate(IV), K ₂ PtCl ₆ ). Platinum chloride (platinum(II) chloride, PtCl ₂ ) and platinum nitrate (platinum(II) nitrate, Pt(NO ₃ ) ₂ ) may be at least one selected from the group consisting of.

The alginic acid gel complex may be cross-polymerized with stones containing a metal that is a divalent or trivalent cation to remove the stones. More specifically, the alginate gel complex can be completely removed by polymerization with finely pulverized stones to form aggregates.

The alginic acid gel complex may exhibit an antibacterial effect, and in particular, it may exhibit a remarkable antibacterial effect on Escherichia coli and Staphylococcus aureus .

Method for preparing alginate gel complex

The present invention is a cationic cross-linked polymer alginic acid salt (alginate); And it provides a method for preparing an alginic acid gel complex by mixing a metal salt having antibacterial properties.

The alginic acid gel complex is the same as described above.

The alginic acid salt may be sodium alginate having a concentration of 0.5 × 10 ^-2 M to 5.0 × 10 ^-2 M, preferably 0.5 × 10 ^-2 M to 4.0 × 10 ^-2 M alginic acid having a concentration It may be sodium (sodium alginate), and most preferably, it may be sodium alginate (sodium alginate) having a concentration of 1.0 × 10 ^-2 M to 3.5 × 10 ^-2 M.

The metal salt is selected from the group consisting of 0.5 × 10 ^-2 M to 8.0 × 10 ^-2 M concentration of silver (silver, Ag), copper (copper, Cu), zinc (zinc, Zn) and iron (ion, Fe) It may be one or more salts, preferably 0.5 × 10 ^-2 M to 7.0 × 10 ^-2 M concentration may be one or more salts selected from the group consisting of silver, copper, zinc and iron, most preferably 1.0 × 10 It may be at least one salt selected from the group consisting of silver, copper, zinc and iron at a concentration of ^-2 M to 6.5 × 10 ^-2 M.

Advantages and features of the present invention, and a method of achieving them, will become apparent with reference to the embodiments described below in detail. However, the present invention is not limited to the embodiments disclosed below, but may be implemented in various different forms, and only the embodiments allow the disclosure of the present invention to be complete, and common knowledge in the technical field to which the present invention pertains It is provided to fully inform those who have the scope of the invention, and the present invention is only defined by the scope of the claims.

Example. Preparation of alginate gel complex

An alginic acid gel complex according to the present invention was prepared by mixing the components of [Table 1] below.

[Table 1]

Experimental Example 1. Confirmation of aggregate formation by stones

1.1. Confirmation of the formation of calcium oxalate aggregates

In order to confirm the formation of aggregates between calcium oxalate, an example of a powder form of calcium, which can be said to be the main constituent of stones, and the alginate gel complex according to the present invention, between the alginate gel complex according to the present invention (Example 1) and calcium oxalate Formation of aggregates was confirmed, which is shown in FIG. 1 .

Referring to FIG. 1 , it can be confirmed that an aggregate between the alginate gel complex (Example 1) and calcium oxalate according to the present invention is formed.

1.2. Confirm the formation of stone aggregates

In order to confirm the formation of aggregates between the actual stones and the alginate gel complex according to the present invention, the formation of aggregates between the alginate gel complex according to the present invention (Example 1) and the stones of an actual urolithiasis patient was confirmed, which is shown in FIG. indicated.

Referring to FIG. 2 , it can be confirmed that an aggregate is formed between the alginate gel complex according to the present invention (Example 1) and the stone of an actual urolithiasis patient.

From the above results, it can be confirmed that the alginic acid gel complex according to the present invention can completely remove stones without residue.

Experimental Example 2. Confirmation of antibacterial effect

2.1. Escherichia coli Check antibacterial effect

In order to confirm the antibacterial effect of the alginic acid gel complex according to the present invention, the antibacterial effect was confirmed when Escherichia coli was inoculated into Examples 1 to 3, which are the alginic acid gel complexes according to the present invention, and this is shown in FIG. 3 .

Referring to Figure 3, (a) in the case of the control medium containing the alginate gel complex according to the present invention, it can be confirmed that a large amount of Escherichia coli is present. On the other hand, in the alginic acid gel complex prepared in (b) Example 1, (c) Example 2 and (d) Example 3 according to the present invention, it can be confirmed that the inoculated Escherichia coli was not confirmed at all.

2.2. Staphylococcus aureus Check antibacterial effect

In order to confirm the antibacterial effect of the alginate gel complex according to the present invention, the antibacterial effect was confirmed when Staphylococcus aureus was inoculated into Examples 1 to 3, which are the alginate gel complexes according to the present invention, and this is shown in FIG. 4 .

Referring to FIG. 4 , (a) it can be confirmed that a large amount of Staphylococcus aureus is present in the control medium that does not contain the alginic acid gel complex according to the present invention. On the other hand, in the alginic acid gel complex prepared in (b) Example 1, (c) Example 2 and (d) Example 3 according to the present invention, it can be seen that the inoculated Staphylococcus aureus was not confirmed at all.

From the above results, it can be confirmed that the alginate gel complex according to the present invention has an antibacterial effect and, in particular, has an excellent antibacterial effect on Escherichia coli and Staphylococcus aureus .

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention may be implemented in other specific forms without changing the technical spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not restrictive.

Claims (5)

alginate, which is a cationic cross-linked polymer; and
Alginic acid gel complex comprising a; metal salt having antibacterial properties. The method of claim 1,
The alginic acid gel complex is an alginate gel complex, characterized in that it is cross-polymerized with stones containing a metal that is a divalent or trivalent cation to remove the stones. The method of claim 1,
The alginic acid salt is 0.5 × 10 ^-2 M to 5.0 × 10 ^-2 M sodium alginate (sodium alginate) having an alginate gel complex, characterized in that. The method of claim 1,
The metal salt is one or more salts selected from the group consisting of silver (silver, Ag), copper (copper, Cu), zinc (zinc, Zn), platinum (Pt) and iron (ion, Fe), characterized in that Alginate gel complex. alginate, which is a cationic cross-linked polymer; and a method for preparing an alginate gel complex by mixing a metal salt having antibacterial properties.

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