KR102580016B1 - Alginate gel complex for removing microcalcium stones and method for preparing thereof - Google Patents

Abstract

The present invention relates to an alginate gel complex for removing stones and a method for manufacturing the same. More specifically, the present invention relates to a biomaterial that completely removes stones composed of calcium, oxalic acid, uric acid, etc. as main ingredients without remaining stones and prevents urinary tract infections after surgery. It relates to an alginic acid gel composite for removing stones that is friendly and has an antibacterial effect at the same time, and a method for manufacturing the same.

Description

Alginate gel complex for removing microcalcium stones and method for preparing the same}

The present invention relates to an alginate gel complex for removing stones and a method for manufacturing the same. More specifically, the present invention relates to a biomaterial that completely removes stones composed of calcium, oxalic acid, uric acid, etc. as main ingredients without remaining stones and prevents urinary tract infections after surgery. It relates to an alginic acid gel composite for removing stones that is friendly and has an antibacterial effect at the same time, and a method for manufacturing the same.

Urinary nodules are a common disease in urology, accounting for more than 25% of patients visiting urology, a symptom of developed countries that steadily increases due to improvements in living standards and changes in diet.

The main ingredients that make up urinary stones are calcium, oxalic acid, phosphoric acid, and uric acid, and in rare cases, magnesium ammonium phosphate and cystine. Urinary stones can easily form when these components are excreted excessively in the urine, and factors that promote crystallization and crystallization include urine acidity, urinary tract infection, and urinary tract retention. When these urinary stones occur, the most common symptoms are colic and hematuria. Colic is caused by excessive peristalsis of the urinary collecting system muscles due to sudden urinary tract obstruction, renal capsule, and renal pelvis. This pain appears in the back, sides, and abdomen, and is so severe that the patient's pain cannot be expressed in words. Pain can be continuous or intermittent and is accompanied by nausea, vomiting, and hematuria, and may also cause other urinary disorders or pain during urination.

In general, stones are classified into kidney stones, ureter stones, bladder stones, etc. depending on the area of formation. The most commonly used treatment methods for these are extracorporeal shock wave lithotripsy, a non-invasive stone crushing method, and skin incision of about 1-2 cm. Percutaneous lithotripsy, which involves inserting an endoscope to remove stones, and a method of removing stones through open surgery are being used.

However, it is almost impossible to completely remove small crushed stones even with surgical methods. In addition, the remaining stones may cause the problem of recurrence of stones in the future, and complications due to urinary tract infection may occur due to bacterial spread due to stone obstruction.

Therefore, in order to solve the above-mentioned problems, the present inventors recognized that it is urgent to develop a stone removal method that can completely remove stones without remaining stones and prevent complications due to urinary tract infection after surgery, and completed the present invention.

Republic of Korea Patent Publication No. 10-1994310 Republic of Korea Patent Publication No. 10-14188090

The purpose of the present invention is to completely remove stones consisting of calcium, oxalic acid, uric acid, etc. as main ingredients without any remaining stones, and to provide an alginate gel complex and its It provides a manufacturing method.

The technical problems to be achieved by the invention are not limited to the technical problems mentioned above, and other technical problems not mentioned can be clearly understood by those skilled in the art from the description of the present invention.

In order to achieve the above object, the present invention provides an alginate gel composite for removing stones and a method for producing the same.

Hereinafter, this specification will be described in more detail.

The present invention relates to alginate, which is a cationic cross-linked polymer; and a metal salt having antibacterial properties.

In the present invention, the alginate gel complex is characterized in that the stone is removed by cross-polymerizing with the stone containing a metal that is a divalent or trivalent cation.

In the present invention, the alginate is characterized in that it is sodium alginate having a concentration of 0.5 × 10 ^-2 M to 5.0 × 10 ^-2 M.

In the present invention, the metal salt is one or more selected from the group consisting of silver (Ag), copper (Cu), zinc (Zn), platinum (Pt), and iron (ion, Fe). It is characterized by being salt.

In addition, the present invention includes alginate, which is a cationic cross-linked polymer; and a method of producing an alginic acid gel composite by mixing metal salts with antibacterial properties.

All matters mentioned in the above alginate gel composite for removing stones and its manufacturing method apply equally unless contradictory.

The alginate gel complex for removing stones of the present invention and its manufacturing method completely removes stones composed of calcium, oxalic acid, uric acid, etc. as main ingredients without any remaining stones, and has a biocompatible and antibacterial effect that can prevent postoperative urinary tract infections at the same time. It can be expressed.

The effects of the present invention are not limited to the effects mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the description of the claims.

Figure 1 is an image confirming the formation of aggregates between an alginate gel composite (Example 1) and calcium oxalate according to the present invention.
Figure 2 is an image confirming the formation of aggregates between (a) a stone from an actual urinary stone patient and (b) an alginate gel composite (Example 1) according to the present invention.
Figure 3 shows (a) a control group containing only Escherichia coli without the alginate gel complex according to the present invention, (b) Example 1, (c) Example 2, and (d) Example 3 in which Escherichia coli was attached. This image confirms the antibacterial effect of the prepared alginate gel complex.
Figure 4 shows (a) a control group containing only Staphylococcus aureus without the alginate gel complex according to the present invention, (b) Example 1, (c) Example 2, and (d) Example 3 in which Staphylococcus aureus was attached. This image confirms the antibacterial effect of the prepared alginate gel complex.

The terms used in this specification are general terms that are currently widely used as much as possible while considering the function in the present invention, but this may vary depending on the intention or precedent of a person skilled in the art, the emergence of new technology, etc. In addition, in certain cases, there are terms arbitrarily selected by the applicant, and in this case, the meaning will be described in detail in the description of the relevant invention. Therefore, the terms used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, rather than simply the name of the term.

Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by a person of ordinary skill in the technical field to which the present invention pertains. Terms defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related technology, and unless clearly defined in the present application, should not be interpreted in an ideal or excessively formal sense. No.

The numerical range includes the values defined in the range above. Every maximum numerical limit given throughout this specification includes all lower numerical limits as if the lower numerical limit were explicitly written out. Every minimum numerical limit given throughout this specification includes every higher numerical limit as if such higher numerical limit was clearly written. All numerical limits given throughout this specification will include all better numerical ranges within the broader numerical range, as if the narrower numerical limits were clearly written.

Hereinafter, examples of the present invention will be described in detail, but it is obvious that the present invention is not limited to the following examples.

Alginate Gel Complex

The present invention relates to alginate, a cationic cross-linked polymer; and a metal salt having antibacterial properties.

The alginic acid is a complex polysaccharide acid represented by the following [Formula 1] consisting of mannuronic acid and glucuronic acid, which constitute the cell wall of brown algae. The alginic acid may have a degree of polymerization of 80 and a molecular weight of 20,000 to 240,000.

[Formula 1]

The alginate may be sodium alginate having a concentration of 0.5 × 10 ^-2 M to 5.0 × 10 ^-2 M, preferably alginate having a concentration of 0.5 × 10 ^-2 M to 4.0 × 10 ^-2 M. It may be sodium alginate, and most preferably it may be sodium alginate having a concentration of 1.0 × 10 ^-2 M to 3.5 × 10 ^-2 M.

The metal salt may be one or more salts selected from the group consisting of silver (Ag), copper (Cu), zinc (Zn), platinum (Pt), and iron (ion, Fe). More specifically, the silver salt includes silver nitrate (AgNO ₃ ), silver nitrite (AgNO ₂ ), silver acetate (CH ₃ COOAg), silver perchlorate (AgClO ₄ ), and silver chlorate ( It may be one or more types selected from the group consisting of silver chlorate, AgClO ₃ ), and the copper salt may be copper nitrate (Cu(NO ₃ ) ₂ ), copper chloride (CuCl ₂ ), copper sulfate (copper sulfate, CuSO ₄ ), copper acetate (CH ₃ COO) ₂ Cu), copper acetylacetonate (Cu(O ₂ C ₅ H ₇ ) ₂ ), copper carbonate (CuCO ₃ ), cyclohexane Copper cyclohexane butyrate, (C ₆ H ₁₁ (CH ₂ ) ₃ CO ₂ ) ₂ Cu), copper stearate (C ₁₈ H ₃₅ O ₂ ) ₂ Cu), copper perchlorate (Cu) (ClO ₄ ) ₂ ) and copper(II) hydroxide (Cu(OH) ₂ ). The zinc salt may be selected from the group consisting of zinc chloride (ZnCl ₂ ), zinc sulfate ( zinc sulfate, ZnSO ₄ ), zinc nitrate (Zn(NO ₃ ) ₂ ), zinc acetate (Zn(CH ₃ CO ₂ ) ₂ ), zinc phosphate (zinc phosphate, Zn ₃ (PO ₄ ) ₂ )(H ₂ O) ₄ ), zinc fluoride (ZnF ₂ ), zinc bromide (ZnBr ₂ ), and zinc iodide (zinc iodide, ZnI ₂ ). It may be one or more selected from the group consisting of, The iron salts include iron(III) nitrate, Fe(NO ₃ ) ₃ ), iron chloride (FeCl ₂ or FeCl ₃ ), iron(III) oxide-hydroxide (Fe(OH) ₃ ), and sulfuric acid. It may be one or more selected from the group consisting of iron (iron(II) sulfate, FeSO ₄ ), and the platinum salt may be sodium tetrachloroplatinate (II), Na ₂ PtCl ₄ ), potassium tetrachloroplatinate ( potassium tetrachloroplatinate, K ₂ PtCl ₄ ), sodium hexachloroplatinate(IV), Na ₂ PtCl ₆ ), potassium hexachloroplatinate(IV), K ₂ PtCl ₆ ). It may be one or more selected from the group consisting of platinum(II) chloride (PtCl ₂ ) and platinum(II) nitrate (Pt(NO ₃ ) ₂ ).

The alginate gel complex can remove stones by cross-polymerizing stones containing metals that are divalent or trivalent cations. More specifically, the alginate gel composite can completely remove the crystals by polymerizing with finely ground stones to form aggregates.

The alginate gel complex may exhibit an antibacterial effect, and may especially exhibit a significant antibacterial effect against Escherichia coli and Staphylococcus aureus .

Method for producing alginate gel composite

The present invention relates to alginate, a cationic cross-linked polymer; and a method of producing an alginic acid gel composite by mixing metal salts with antibacterial properties.

The alginate gel complex is as mentioned above.

The alginate may be sodium alginate having a concentration of 0.5 × 10 ^-2 M to 5.0 × 10 ^-2 M, preferably alginate having a concentration of 0.5 × 10 ^-2 M to 4.0 × 10 ^-2 M. It may be sodium alginate, and most preferably it may be sodium alginate having a concentration of 1.0 × 10 ^-2 M to 3.5 × 10 ^-2 M.

The metal salt is selected from the group consisting of silver (Ag), copper (Cu), zinc (Zn), and iron (ion, Fe) at a concentration of 0.5 × 10 ^-2 M to 8.0 × 10 ^-2 M. It may be one or more salts, preferably one or more salts selected from the group consisting of silver, copper, zinc and iron at a concentration of 0.5 × 10 ^-2 M to 7.0 × 10 ^-2 M, most preferably 1.0 × 10 It may be one or more salts selected from the group consisting of silver, copper, zinc and iron at a concentration of ^-2 M to 6.5 × 10 ^-2 M.

The advantages and features of the present invention and methods for achieving them will become clear with reference to the embodiments described in detail below. However, the present invention is not limited to the embodiments disclosed below and may be implemented in various different forms, and only the embodiments are provided to ensure that the disclosure of the present invention is complete, and are provided by those skilled in the art It is provided to fully inform those who have the scope of the invention, and the present invention is only defined by the scope of the claims.

Example. Alginate gel composite preparation

An alginate gel composite according to the present invention was prepared by mixing the components shown in Table 1 below.

[Table 1]

Experimental Example 1. Confirmation of aggregate formation by stones

1.1. Confirmation of formation of calcium oxalate aggregates

In order to confirm the formation of aggregates between calcium oxalate, which is an example of the powder form of calcium, which can be said to be the main component of stones, and the alginate gel composite according to the present invention, the alginate gel composite according to the present invention (Example 1) and calcium oxalate The formation of aggregates was confirmed, and this is shown in Figure 1.

Referring to Figure 1, it can be seen that an aggregate was formed between the alginate gel composite according to the present invention (Example 1) and calcium oxalate.

1.2. Confirmation of stone aggregate formation

In order to confirm the formation of aggregates between actual stones and the alginate gel composite according to the present invention, the formation of aggregates between the alginate gel composite according to the present invention (Example 1) and stones from actual urinary stone patients was confirmed, and this is shown in Figure 2 indicated.

Referring to Figure 2, it can be seen that aggregates were formed between the alginate gel composite according to the present invention (Example 1) and the stones of actual urinary stone patients.

From the above results, it can be confirmed that the alginate gel complex according to the present invention can completely remove stones without leaving any residue.

Experimental Example 2. Confirmation of antibacterial effect

2.1. Escherichia coli Confirmation of antibacterial effect

In order to confirm the antibacterial effect of the alginic acid gel complex according to the present invention, the antibacterial effect was confirmed when Escherichia coli was inoculated into Examples 1 to 3, which are the alginic acid gel complex according to the present invention, and this is shown in Figure 3.

Referring to Figure 3, (a) it can be seen that a large amount of Escherichia coli is present in the control medium that does not contain the alginate gel complex according to the present invention. On the other hand, it can be seen that no inoculated Escherichia coli was found in the alginate gel composites prepared in (b) Example 1, (c) Example 2, and (d) Example 3 according to the present invention.

2.2. Staphylococcus aureus Confirmation of antibacterial effect

In order to confirm the antibacterial effect of the alginic acid gel complex according to the present invention, the antibacterial effect was confirmed when Staphylococcus aureus was inoculated into Examples 1 to 3, which are the alginic acid gel complex according to the present invention, and this is shown in Figure 4.

Referring to Figure 4, (a) it can be seen that a large amount of Staphylococcus aureus is present in the control medium that does not contain the alginate gel complex according to the present invention. On the other hand, it can be confirmed that no inoculated Staphylococcus aureus was found in the alginate gel composites prepared in (b) Example 1, (c) Example 2, and (d) Example 3 according to the present invention.

From the above results, it can be confirmed that the alginate gel complex according to the present invention has an antibacterial effect, especially excellent antibacterial effect against Escherichia coli and Staphylococcus aureus .

From the above description, those skilled in the art to which the present invention pertains will understand that the present invention can be implemented in other specific forms without changing its technical idea or essential features. In this regard, the embodiments described above should be understood in all respects as illustrative and not restrictive.

Claims (5)

alginate, a cationic cross-linked polymer; and
Includes a metal salt having antibacterial properties,
The alginate is sodium alginate having a concentration of 0.5 × 10 ^-2 M to 5.0 × 10 ^-2 M,
An alginic acid gel composite, wherein the metal salt is silver (Ag). delete delete delete alginate, a cationic cross-linked polymer; and a method of producing an alginic acid gel composite by mixing metal salts with antibacterial properties,
The alginate is sodium alginate having a concentration of 0.5 × 10 ^-2 M to 5.0 × 10 ^-2 M,
The metal salt is
A method of producing an alginate gel composite characterized in that it is silver (Ag).