KR20220089816A - Cosmetic composition for improving atopic dermatitis containing anti-inflammatory herbal fermented peptide and method for manufacturing the same - Google Patents

Abstract

The cosmetic composition for improving atopic dermatitis according to the present invention contains an anti-inflammatory peptide isolated from fermented beans, and thus has an excellent effect in improving, alleviating, and treating diseases that cause inflammation in the skin, particularly atopic dermatitis, and has a skin soothing effect and It has excellent moisturizing properties, and even when used in various formulations, the excellent initial effect is maintained even after long-term storage. In addition, the cosmetic composition for improving atopic dermatitis comprising the anti-inflammatory oriental fermented peptide according to the present invention has the advantage that it is cheaper and can be supplied in large quantities.

Description

Cosmetic composition for improving atopic dermatitis containing anti-inflammatory herbal fermented peptide and method for manufacturing the same

The present invention relates to a cosmetic composition for improving atopic dermatitis comprising an anti-inflammatory oriental fermented peptide and a method for preparing the same.

Recently, social and economic growth, industrialization, urbanization and dietary habits, changes in the residential environment, environmental changes such as fine dust and yellow dust, and climate change due to global warming are the causes of atopic diseases. In addition, serious skin diseases are occurring in the local community due to the rapid deterioration of the environment due to fine dust and the like. Therefore, improvement products for the continuous improvement of symptoms of skin diseases are being researched and developed.

Among skin diseases, atopic dermatitis is a worldwide common disease that can occur at any age, and it occurs a lot in childhood and adolescence. If not, it leads to adulthood, and in many cases it progresses to severe disease and worsens. Also, in Korea, the problem of atopic dermatitis has already reached a serious social and medical level. As a result of the 2015 survey on the prevalence of allergic diseases by region, among middle school students, Chungcheong (Daejeon, Chungnam, and North Chungcheong) had the highest at 33.7%, and the major diseases were allergic rhinitis (30%), asthma (35%), and atopic dermatitis (48.6%) for children under 12 years of age. appears to be, and measures are urgently needed.

As such, atopic dermatitis is becoming a serious social problem by causing serious problems in social life such as employment and marriage in severe cases, as well as in children as well as in adults. In this case, severe mental stress can cause social avoidance and depression. Therefore, for atopic dermatitis to be treated and improved, it is most important to block stimulation from environmental factors and to perform continuous management, and research on the development of various products is required for this purpose.

Atopic dermatitis is a chronic inflammatory skin disease that repeats improvement and exacerbation of symptoms due to a combination of environmental, social, psychological, and genetic factors, with an imbalance in immune function as the main cause. Mast cell-derived inflammatory cytokines further promote the inflammatory response, while itch-inducing substances such as histamine further promote itching and cause the skin barrier to collapse.

Therefore, the discovery of natural substances that have fewer side effects, enhance immune function, and have anti-allergic and skin irritation inhibitory effects is a fundamental treatment that can replace existing non-specific and popular drugs in skin inflammatory diseases such as atopic dermatitis. can be

Current anti-inflammatory agents are mainly to produce inflammatory mediators for inflammatory diseases and to inhibit the factors. However, currently discovered anti-inflammatory drugs, such as aspirin, nonsteroidal anti-inflammatory drugs and Cox-2 inhibitors, have the disadvantage that they cannot be used for certain patients. In addition, most anti-inflammatory agents specifically target and inhibit inflammatory mediators secreted from inflammatory cells in the inflammatory site, and these drugs, including aspirin, NSAIDS, and steroid Cox-2, are expensive and No, it is highly toxic.

Migration of T lymphocytes to the inflamed site in most cases of inflammatory dermatitis and psoriasis, nasal mucus due to allergic reaction of rhinitis, lung asthma and rheumatoid arthritis, etc. is an important factor accompanying inflammation pathologically. The development of novel peptides for improvement is required.

The properties of anti-inflammatory peptides have a very wide range of anti-inflammatory action compared to existing antibiotics, their length is as short as 20-40 amino acids, and they are almost unaffected by acid/alkali and heat treatment, and secondary modifications ( Secondary modification) is rare. In addition, these anti-inflammatory peptides are substances composed of amino acids, and are more environmentally friendly than existing antibiotics because they can be easily decomposed after anti-inflammatory action, thereby reducing the risk of environmental destruction.

Common structural characteristics of anti-inflammatory peptides include base amino acids such as lysine and arginine, and have an amphiphatic structure including α-helix. These anti-inflammatory peptides are positively charged and bind to the cell wall of negatively charged microorganisms and are arranged at the same time, forming an α-helix structure, with one hydrophobic end penetrating the cell wall and making a hole to release intracellular substances or decompose the cell wall This will kill the bacteria.

The activity of peptide anti-inflammatory substances differs depending on the structure and composition of the microbial cell wall, and the separated peptides have almost different structures depending on the source of separation, so their composition is very diverse. Since the types of invading pathogens are different depending on the environment in which each organism grows, the types of peptides acting on it are also different and have diversity.

As a result of many studies on anti-inflammatory peptides, several kinds of anti-inflammatory peptides are newly discovered every year, and their molecular biology studies and their potential as new anti-inflammatory agents useful in the human body are being intensively studied. However, since anti-inflammatory peptides with the same effect as existing antibiotics have not been searched for and industrialization has not been conducted, research is focused on the search for new anti-inflammatory peptides.

In the case of such anti-inflammatory peptide materials, materials with functions related to wrinkles and anti-aging were mainly released in cosmetics, but peptide materials targeting atopy improvement are still difficult to popularize. Currently, these peptides are produced by chemical synthesis, and biological mass production methods are being studied in order to supply them in large quantities at a cheaper price. However, since the anti-inflammatory power of these anti-inflammatory peptides is weaker than that of existing antibiotics and there is a problem in securing a large amount, in-depth research is needed to overcome this.

Furthermore, a moisturizer applied to atopic cosmetics is insufficient for a simple moisturizing effect, and a natural moisturizing factor that can control the amount of moisture in the skin is applied, and at the same time, an anti-inflammatory peptide excellent in relieving inflammation and cosmetics containing the same. development is required.

Korean Patent Publication No. 10-2017-0095433

An object of the present invention is to provide a cosmetic composition for improving atopic dermatitis comprising an anti-inflammatory oriental fermented peptide excellent in the improvement, alleviation, and treatment of diseases that cause inflammation in the skin, particularly atopic dermatitis, and a method for manufacturing the same.

Another object of the present invention is to provide a cosmetic composition for improving atopic dermatitis comprising an anti-inflammatory oriental medicinal fermented peptide that can be supplied in large quantities and inexpensively, and a method for preparing the same.

Another object of the present invention is to provide a cosmetic composition for improving atopic dermatitis comprising an anti-inflammatory oriental medicinal fermented peptide having excellent skin soothing effect and moisturizing properties, and a method for preparing the same.

Another object of the present invention is a cosmetic composition for improving atopic dermatitis comprising an anti-inflammatory oriental fermented peptide, which has excellent initial effects of skin soothing properties, moisturizing properties and atopic dermatitis improvement properties even after being used in various formulations, even after long-term storage, and a method for manufacturing the same is to provide

The cosmetic composition for improving atopic dermatitis according to the present invention includes an anti-inflammatory peptide isolated from fermented beans.

The cosmetic composition for improving atopic dermatitis according to an embodiment of the present invention may further include a fermented product obtained by fermenting an extract obtained by heat-extracting a member of the sea.

In an embodiment of the present invention, the fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented lactic acid bacteria may be fermented with lactic acid bacteria.

In one embodiment of the present invention, the anti-inflammatory peptide, a fermentation step of fermenting a mixture containing a legume raw material and water, an extraction step of preparing a fermented extract obtained by extracting the fermented product obtained in the fermentation step with an extraction solvent, and the fermented extract It may be prepared including a separation step of isolating the anti-inflammatory peptide from

In one embodiment of the present invention, in the fermentation step, the mixture may be a mixture comprising a legume raw material and water at 70 to 150 ℃ heat treatment.

In one embodiment of the present invention, in the fermentation step, the fermentation may be performed at 30 to 45 ℃.

In an example of the present invention, in the extraction step, the extraction solvent may include ethanol, and the extraction may be thermal extraction.

In one embodiment of the present invention, the anti-inflammatory peptide, between the extraction step and the separation step, may be prepared by further comprising the step of pulverizing the ferment extract.

In one embodiment of the present invention, the separation step may be a step of extracting and separating the anti-inflammatory peptide using a chromatography method on the ferment extract powder obtained in the pulverizing step.

In one embodiment of the present invention, in the fermentation step, the legume raw material may include soybeans.

The present invention may provide an external preparation for skin comprising the cosmetic composition for improving atopic dermatitis.

The cosmetic composition for improving atopic dermatitis comprising the anti-inflammatory oriental fermented peptide according to the present invention has an excellent effect in improving, alleviating, and treating diseases that cause inflammation in the skin, in particular, atopic dermatitis.

The cosmetic composition for improving atopic dermatitis containing the anti-inflammatory oriental fermented peptide according to the present invention has the advantage of being cheaper and available in large quantities.

The cosmetic composition for improving atopic dermatitis containing the anti-inflammatory oriental fermented peptide according to the present invention has an excellent skin soothing effect and moisturizing properties.

Even when the cosmetic composition for improving atopic dermatitis comprising the anti-inflammatory oriental fermented peptide according to the present invention is used in various formulations, the initial excellent effects of skin soothing properties, moisturizing properties and atopic dermatitis improvement properties persist even after long-term storage.

1 is an image schematically showing the composition and effect of a cosmetic composition for improving atopic dermatitis comprising an anti-inflammatory oriental fermented peptide according to the present invention.

Hereinafter, a cosmetic composition for improving atopic dermatitis comprising an anti-inflammatory oriental fermented peptide according to the present invention and a manufacturing method thereof will be described in detail with reference to the accompanying drawings.

The drawings described in this specification are provided as examples in order to sufficiently convey the spirit of the present invention to those skilled in the art. Therefore, the present invention is not limited to the drawings presented and may be embodied in other forms, and the drawings may be exaggerated to clarify the spirit of the present invention.

Unless otherwise defined in technical terms and scientific terms used in this specification, those of ordinary skill in the art to which this invention belongs have the meanings commonly understood, and in the following description and accompanying drawings, the subject matter of the present invention Descriptions of known functions and configurations that may unnecessarily obscure will be omitted.

A singular form of a term used herein may be construed to include a plural form as well unless otherwise indicated.

Unless otherwise specified, the unit of % used in the present specification means % by weight unless otherwise specified.

The cosmetic composition for improving atopic dermatitis according to the present invention contains an anti-inflammatory peptide isolated from fermented beans, and has an excellent effect in improving, alleviating, and treating diseases that cause inflammation in the skin, in particular, atopic dermatitis.

Hereinafter, a method for producing an anti-inflammatory peptide isolated from a fermented pulse will be described in detail.

The anti-inflammatory peptide according to the present invention includes a fermentation step of fermenting a mixture containing legume raw materials and water, an extraction step of preparing a fermented extract obtained by extracting the fermented product obtained in the fermentation step with an extraction solvent, and separating the anti-inflammatory peptide from the fermented extract It can be prepared including a separation step. In this case, it is preferable that the legume raw material is soybean.

The fermentation step is a step of fermenting a mixture including a legume raw material and water. In this case, natural fermentation is also possible, and lactic acid bacteria fermentation by artificially adding lactic acid bacteria is also possible.

In the fermentation step, fermentation conditions may use known fermentation conditions, for example, may be carried out at 20 to 50 ° C., specifically 30 to 45 ° C., and the fermentation time is 10 to 120 hours, specifically 20 to 90 hours. It can be fermented while The fermented product prepared in this way may refer to cheonggukjang, which is commonly known as a material in which whole beans are fermented at room temperature for 1 to 4 days.

The method for producing an anti-inflammatory peptide according to the present invention, before the fermentation step, the mixture may further include a heat treatment step of heat-treating the mixture including the legume raw material and water at 70 to 150 ℃. Through this, the subsequent fermentation can be performed more effectively, and the anti-inflammatory peptide can be separated in a more stable and high yield when the anti-inflammatory peptide is isolated thereafter.

The method for producing an anti-inflammatory peptide according to the present invention may further include a water immersion step of immersing the legume raw material in water for a predetermined time before the fermentation step or before the heat treatment step. The immersion temperature is preferably in the room temperature range, for example, 10 to 35 ℃ can be mentioned. The immersion time may be as long as moisture can sufficiently penetrate into the legume raw material, for example, 5 to 50 hours. However, this is only described as a preferred example, and the present invention is not construed as being limited thereto. Through this, subsequent fermentation can be performed more effectively.

The extraction step is a step of extracting the anti-inflammatory peptide from the fermented product that has undergone the fermentation step, and the extraction method is not particularly limited, but examples include heat extraction, low temperature extraction, room temperature extraction, etc., and preferably room temperature extraction. have.

As a preferred example, when the extraction solvent includes an aqueous ethanol solution, the extraction solvent may include an aqueous ethanol solution having a concentration of 50 to 90% by weight. When an aqueous ethanol solution having a concentration in the above range is used, anti-inflammatory properties can be significantly improved by extracting the anti-inflammatory peptide from the extraction target in a high content.

The content of the extraction solvent may be sufficient as long as the anti-inflammatory peptide can be extracted with the extraction solvent, for example, 3 to 30 parts by weight based on 1 part by weight of the fermented product. However, this is only described as a specific example, of course, the present invention is not limited thereto.

The extract obtained in the extraction step can be separated and obtained as it is through the separation step, but the anti-inflammatory peptide can be isolated and obtained after purification, for example, through various known methods, for example, by the following means. .

The method for producing an anti-inflammatory peptide according to an embodiment of the present invention may further include a filtration step of removing solid substances such as residues remaining in a solid phase on the extraction solvent after the extraction is completed in the extraction step. In this case, the filtration particle size is not particularly limited, and may be, for example, 0.1 to 30 µm, specifically 0.5 to 10 µm, but is not limited thereto.

In addition, the method for producing an anti-inflammatory peptide according to an embodiment of the present invention may further include, between the extraction step and the separation step, a concentration step of removing the extraction solvent from the solid-containing extract that has undergone the extraction step, for example , the solvent removal step may be performed through concentration under reduced pressure.

When the concentration step is further performed, the concentration under reduced pressure may be performed under vacuum conditions, for example, at 10 mmHg to 700 mmHg, and may be performed within the range of 10 to 100° C. under vapor temperature conditions. However, this is only described as a preferred example, of course, the present invention is not limited thereto.

In addition, the method for producing an anti-inflammatory peptide according to an embodiment of the present invention, between the extraction step and the separation step, may be prepared by further comprising the step of pulverizing the ferment extract. Various known means may be used for the powdering step, and an example may be freeze-drying. In the case of further freeze-drying, as a specific example, the freezing temperature may be -30 to -80°C, the drying pressure may be 0.1 to 100 torr, and the freezing and drying time may be independently of each other 5 to 30 hours. , of course, the present invention is not limited thereto.

The separation step may be a step of extracting and separating the anti-inflammatory peptide from the fermented product obtained through the extraction step using chromatography. In the extraction step, since the anti-inflammatory peptide is extracted together with other components into the extraction solvent, it is necessary to separate the anti-inflammatory peptide from the mixture containing several components. Specifically, it is possible to isolate and obtain an anti-inflammatory peptide from a fermentation product using high-performance liquid chromatography (HPLC). Preferably, it is preferable to use acetonitrile (CH ₃ CN) containing trifluoroacetic acid as a solvent, and the concentration may be 0.01 to 3% by weight, at 25°C and 1 atm. It can be carried out at a flow rate of 0.05 to 3 mL/min.

The cosmetic composition for improving atopic dermatitis according to a preferred embodiment may further include a fermented product obtained by fermenting the extract obtained by thermally extracting the extract. Specifically, the anti-inflammatory cosmetic composition according to the present invention comprises a fermented fermented fermented fermented extract obtained by heat extraction of fermented soybeans with an anti-inflammatory peptide isolated from fermented legumes, thereby improving, alleviating and treating diseases causing atopic dermatitis. It has excellent effects and excellent skin soothing and moisturizing properties. In addition, the effect can be further improved by going through a means in which the joiner is extracted and fermented by a method to be described later.

The cosmetic composition for improving atopic dermatitis according to the present invention comprises: an anti-inflammatory peptide isolated from fermented beans; and a fermented product obtained by fermenting an extract obtained by heat-extracting a member of the tree; if included, their composition ratio is not particularly limited, for example, the weight ratio is 1:100 to 100:1, specifically 30:70 to 70:30 can be

The joiner (蘇木, Caesalpinia sappan L. ) is a plant belonging to the leguminosae , and the stem and thick branches of the joiner are used. , redwood (赤木), redwood (紅紫), etc., can be used by peeling and drying the bright scarlet wood for medicinal purposes.

Hereinafter, a method for producing a fermented product according to the present invention will be described in detail.

As a preferred embodiment, the method for producing a fermented fermented product according to the present invention may preferably include an extraction step of preparing an extract by heat-extracting a fermented fermented fermented mulberry with an extraction solvent containing ethanol, and a fermentation step of fermenting the extract with lactic acid bacteria. have.

In the extraction step, the extraction means is preferably hot water extraction using an extraction solvent containing water. That is, in the case of hot water extraction using an aqueous ethanol solution as an extraction solvent, the active ingredients implementing the above-described characteristics are extracted in a high content from each extraction target in the extraction solvent, thereby remarkably improving anti-dermatitis and moisturizing properties.

As a preferred example, when the extraction solvent includes an aqueous ethanol solution, the extraction solvent may include an aqueous ethanol solution having a concentration of 50 to 90% by weight. When an aqueous ethanol solution is used as an extraction solvent, when an aqueous ethanol solution having a concentration in the above range is used, it may be preferable because the active ingredients implementing the above-described properties and effects can be extracted at a higher content.

The amount of the extraction solvent used may be sufficient as long as the above-mentioned active ingredients can be extracted from the joiner, for example, 3 to 30 parts by weight based on 1 part by weight of the joiner. However, this is only described as a specific example, of course, the present invention is not limited thereto.

In the extraction step, heat extraction conditions may be any known heat extraction conditions. For example, the extraction temperature may be 50 to 150° C., and the extraction time may be 1 to 5 hours. However, this is only described as a preferred example, and the present invention is not construed as being limited thereto.

The joiner may be pulverized and used for extraction with an increased surface area in order to improve extraction efficiency. That is, the method for producing a fermented material of the present invention may further include a grinding step of pulverizing the joiner before the extraction step. At this time, the size of each pulverized extraction target may be as long as it is easy to extract from the extraction solvent, for example, 0.5 to 3 cm, but this is only described as a specific example, and the present invention is not limited thereto. .

The extract obtained in the extraction step may be used as it is in the subsequent fermentation step, but may be purified through various known methods, for example, by the following means, and then subjected to the fermentation step.

The method for producing a fermented joiner according to an embodiment of the present invention may further include a filtering step of removing solid substances such as joiner residues remaining in a solid phase on the extraction solvent after the extraction is completed in the extraction step. In this case, the filtration particle size is not particularly limited, and may be, for example, 3 to 30 μm, but is not limited thereto.

In addition, the method for producing a fermented fermented material according to an embodiment of the present invention may further undergo a solvent removal step of removing the extraction solvent from the solid-containing extract that has undergone the extraction step, for example, the solvent removal step is performed through concentration under reduced pressure. can be That is, the method for producing a fermented fermented material according to an embodiment of the present invention may further include, after the extraction step, a concentration step of concentrating the extract under reduced pressure to prepare a concentrate. The purified product that has undergone this solvent removal step may be subjected to a subsequent fermentation step.

When the concentration step is further performed, the concentration under reduced pressure may be performed under vacuum conditions, for example, at 10 mmHg to 700 mmHg, and may be performed within the range of 10 to 100° C. under vapor temperature conditions. However, this is only described as a preferred example, of course, the present invention is not limited thereto.

In addition, the method for producing a fermented fermented material according to an embodiment of the present invention may further include the step of sterilizing the extract or the purified product of the extract. The purified product that has undergone this sterilization step is subjected to a subsequent fermentation step. The sterilization means for the sterilization step may be any means capable of creating an environment capable of killing bacteria, for example, atmospheric sterilization or autoclaving, and more specifically, at 1 to 10 atm and 100 to 150° C. The heat treatment performed can be exemplified. However, this is only described as a specific example, and the present invention is not limited thereto.

The method for producing a fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented by fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented fermented peas, may be freeze-dried by freeze-drying. That is, in some cases, the dried product obtained by freeze-drying the extract obtained through the extraction step may be subjected to the fermentation step of the subsequent process, and the dried product obtained by freeze-drying the fermented fermented material obtained through the fermentation step is a cosmetic composition may be applied to When the freeze-drying is further performed, as a specific example, the freezing temperature may be -30 to -80°C, the drying pressure may be 0.1 to 100 torr, and the freezing and drying time may be independently of each other 5 to 30 hours. However, of course, the present invention is not limited thereto.

The fermentation step is a step of fermenting the extract of the extraction step or a purified product of the extract, and fermentation may use lactic acid bacteria. Various types of the lactic acid bacteria may be used, but for example, Lactobacillus salivarius , Lactobacillus acidophilus , Lactobacillus brevis , Lactobacillus rhamnosus , Lactobacillus rhamnosus Bacillus plantarum ( Lactobacillus plantarum ), Lactobacillus helveticus ( Lactobacillus helveticus ), Lactobacillus fermentum ( Lactobacillus fermentum ), Lactobacillus paracasei ( Lactobacillus paracasei ), Lactobacillus casei , Lactobacillus casei ( Lactobacillus casei ), Lactobacillus casei ( Lactobacillus casei ) Lactobacillus delbrueckii ), Lactobacillus reuteri ( Lactobacillus reuteri ), Lactobacillus buchneri ( Lactobacillus buchneri ), Lactobacillus gasseri ( Lactobacillus gasseri ), Lactobacillus johnsny ( Lactobacillus irac ), joobhonson kefir ( ), joobhonson Cocos lactis ( Lactococcus lactis ), Lactococcus plantarum ( Lactococcus plantarum ), Lactococcus raffinolactis ( Lactococcus raffinolactis ), Enterococcus faecalis ( Enterococcus faecalis ), Enterocococcus faecalis ) Thermophilius ( Streptococcus thermophilus ), Leuconostoc lactis ), Leuconostoc mesenteroides ), Bifidobacterium anil muls ( Bifidobacterium animals ), Bifidobacterium bifidium ( Bifidobacterium animals ) ium bifidum), Bifidobacterium breve , Bifidobacterium infantis, Bifidobacterium longum , Bifidobacterium pseudolongum , Bifidobacterium Leum thermophilum ( Bifidobacterium themophilum ) and Bifidobacterium adolescentis ( Bifidobacterium adolescentis ).

In the fermentation step, the fermentation conditions may use known fermentation conditions, for example, the extract or a purified product thereof may be fermented using lactic acid bacteria at 20 to 50° C. for 10 to 100 hours.

The method for producing a fermented product according to an embodiment of the present invention may further include a filtration step of removing a solid material remaining in a solid phase on the fermented product after the fermentation is completed in the fermentation step. In this case, the filtration particle size is not significantly limited, and, for example, nano-unit filter paper of 0.05 to 1 μm may be used.

The cosmetic composition for improving atopic dermatitis according to the present invention may be used as a component contained in a known cosmetic composition, for example, 0.001 to 20% by weight, specifically 0.01 to 10% by weight, more specifically, based on the total weight of the final cosmetic composition As may be included in 0.05 to 3% by weight.

The cosmetic composition for improving atopic dermatitis according to the present invention may further include a known component to be a cosmetic composition suitable for the required formulation. Components and component contents of known cosmetic compositions other than the above-mentioned components may be different depending on the specific use/formulation of the cosmetic composition, and since it belongs to well-known technology in the cosmetic field, a detailed description is omitted, but the object of the present invention can be achieved Various additional ingredients within the range, for example, melanin synthesis inhibitor, anti-aging agent, preservative, isotonic agent, stabilizer, bactericidal agent, wound protectant, wound healing agent, purulent disease agent, anti-inflammatory agent, immunosuppressant, parasitic skin Disease solvent, skin emollient, vitamin, herbal medicine, moisturizer, astringent, refreshing agent, antioxidant, UV absorber, UV scattering agent, preservative, antibacterial agent, chelating agent, surfactant, foaming agent, stabilizer, penetrant, preparation, emulsifier, emulsifier, emulsifier, adhesive , may include any one or two or more components selected from components such as adhesives, fragrances and pigments. It can be used without limitation in an appropriate range because it can be appropriately selected and applied and applied by those skilled in the cosmetic field.

The above-described cosmetic composition for improving atopic dermatitis may be used as an external preparation for the skin, and may be applied and applied after being modified to suit the formulation of a known cosmetic composition. For example, softening lotion, astringent lotion, nourishing lotion, bead cream, BB cream, eye cream, nourishing cream, massage cream, cleansing cream, hand cream, sunscreen cream, cleansing foam, cleansing water, foundation, detergent , soap, treatment, cosmetic liquid, etc., may be prepared and used in various formulations such as liquid, solid, powder, hydrogel, essence, spray, patch, ointment, lotion or pack. In addition to this, it may be formulated and used in any suitable form, and each of these formulations is not limited because it may contain various suitable bases and additives required for the formulation of the formulation.

As a specific example, the cosmetic composition according to the present invention may further include one or more cosmetically acceptable carriers to be formulated in general skin cosmetics, and as conventional components, for example, oil, water, surfactant, and moisturizing agent. , a lower alcohol, a thickener, a chelating agent, a colorant, a preservative, a fragrance, etc. may be appropriately mixed, but is not limited thereto. In addition, since the cosmetically acceptable carrier included in the cosmetic composition according to the present invention varies depending on the formulation, reference may be made to known literature.

As a specific example, when the formulation of the cosmetic composition according to the present invention is an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, Bentonite, silica, talc, zinc oxide, or mixtures thereof may be used.

As a specific example, when the formulation of the cosmetic composition according to the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, or a mixture thereof may be used as a carrier component. , in the case of a spray, may additionally contain a propellant such as chlorofluorohydrocarbon, propane/butane or dimethyl ether.

As a specific example, when the formulation of the cosmetic composition according to the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol , benzyl benzoate, propylene glycol, 1,3-butyl glycol oil may be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbate Fatty acid esters of heartburn can be used.

As a specific example, when the formulation of the cosmetic composition according to the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbate A suspending agent such as bitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

As a specific example, when the formulation of the cosmetic composition according to the present invention is soap, alkali metal salt of fatty acid, fatty acid hemiester salt, fatty acid protein hydrolyzate, isethionate, lanolin derivative, aliphatic alcohol, vegetable oil as a carrier component , glycerol, sugar, and the like may be used.

As a specific example, when the formulation of the cosmetic composition according to the present invention is surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltau as carrier components Late, sarcositate, fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester and the like can be used.

The cosmetic composition according to an embodiment of the present invention may be prepared in a bead-type formulation, and in this case, a bead-type cosmetic composition that can be identified for each use by containing a component capable of realizing a desired color, such as a pigment. can As a specific example, there may be provided a bead-type cosmetic composition comprising a bead-type cosmetic composition having a first color and a bead-type cosmetic composition having a second color different from the first color. In this case, the composition and/or composition ratio of the bead-type cosmetic composition having the first color and the bead-type cosmetic composition having the second color may be different to have efficacy/properties suitable for each use. The bead-type capsule of the bead-type cosmetic composition having a first color is used during the day, and the bead-type capsule of the bead-type cosmetic composition having the second color is used at night (used to apply before going to bed at night), etc. can do.

For the preparation method of the bead-type cosmetic composition, reference may be made to the known literature, and specific examples include: a) an aqueous phase in which a mixture containing a thickener, an emulsifier and a fixing agent is first dispersed; and an oil phase in which a base oil, a softening agent and an emulsifier are dissolved; b) mixing the oil phase with the aqueous phase to prepare a liquid emulsion; It may include the step of preparing the emulsion beads. Here, the cosmetic composition and/or the pigment according to the present invention may be mixed and used in any one or two selected from the aqueous phase or the oil phase. At this time, since the content used of each component varies depending on the type of component, etc., it is not limited because a person skilled in the art can easily control it through known literature or experiments. In addition, the size of the emulsion beads may be appropriately adjusted, for example, 0.5 to 50 mm in size, but is not limited thereto.

The thickener is Carbomer 940, Xanthan Gum, Gelatin, Guar Gum, Methylcellulose, Carbopol Pre-Gel, Carbopol, Hycel, Poly Stearate ), one or more may be selected from the group consisting of.

The emulsifier is Hydrogenated Lecithin (Lipoid S 75-3), Polysorbate 20, 60 and 80 (PolySorbate, Tween 20, 60 and 80), PEG-40 Stearate (PEG-40 Stearate, Myrj 52) ), PEG-100 Stearate, Glyceryl Stearate, Cetearyl Alcohol, Sorbitan Isostearate, Potassium Olivate and At least one type may be selected from the group consisting of decyl glucoside.

The immobilizing agent may be selected from the group consisting of gum substances such as indium and guar gum, carrageenan, agar, and mixtures thereof. Besides that, plant exudates such as gum tragacanth and gum arabic; plant seed rubbery substances such as grasshopper soybean gum and guar gum; semi-synthetic rubbery materials such as CMC (carboxy methyl cellulose), hydroxypropyl cellulose and methyl cellulose; seaweed extracts such as propylene glycol alginate; fermentation products such as xanthan gum and dextran; and low methoxy pectin.

The base oil can be used to maintain a liquid form at room temperature, for example, vegetable oils phyto (phyto) horse oil, almond oil, apricot seed oil, argan oil, avocado oil, borage oil, camellia oil, evening primrose oil It may include any one or two or more selected from seed oil, coconut oil, flaxseed oil, grape seed oil, green tea seed oil, hemp seed oil, jojoba oil, macadamia nut oil, rosehip oil, and sunflower oil.

The softening agent spreads well without stickiness and can use a synthetic oil having a low coagulation point, and at least one kind from the group consisting of cetylethylhexanoate (CEH oil), heptylundecylenate and caprylic/capric triglyceride can be selected.

Hereinafter, the present invention will be described in detail with reference to Examples, but these are for describing the present invention in more detail, and the scope of the present invention is not limited by the Examples below.

<Production of fermented soybean powder composition>

Black beans (Glycine max (L.) Merr.) were immersed in water at 25° C. for 24 hours, and water was mixed with the soaked black beans in a 1:1 weight ratio, followed by heat treatment at 120° C. for 15 minutes. After cooling the heat-treated mixture, it was fermented at 37° C. for 3 days to obtain a fermented product. And the fermented product was extracted with a 10-fold aqueous ethanol solution (70 wt%) at 25° C. for 3 days. Subsequently, the obtained fermented extract was filtered to remove residual solid material, and concentrated under reduced pressure at 80 mmHg and 25° C. with a rotary vacuum, and then frozen at -60° C. with a freeze dryer until the solvent was completely evaporated. It was dried to obtain a bean fermented powder composition.

<Isolation of anti-inflammatory peptides>

Anti-inflammatory peptides were isolated and obtained from the pulse fermented powder composition using high-performance liquid chromatography (HPLC). Specifically, using acetonitrile (CH ₃ CN) containing 0.1% by weight of trifluoroacetic acid as a solvent, using an ODS column (5C18-AR-300, Waters) (4.6 mm) and HPLC was performed at a flow rate of 0.5 mL/min.

And using the anti-inflammatory peptide as a sample, each characteristic, such as skin stability and anti-inflammatory, was measured through an experimental example to be described later. At this time, as a sample, a dilution prepared by mixing the anti-inflammatory peptide in distilled water at a concentration of 1 mg/mL was used.

An anti-inflammatory composition was prepared by mixing the anti-inflammatory peptide of Example 1 and the following fermented fermented material in a 1:1 weight ratio.

And, using the anti-inflammatory composition as a sample, each characteristic such as skin stability and anti-inflammatory was measured through an experimental example to be described later. At this time, a diluted solution prepared by mixing the anti-inflammatory composition with distilled water at a concentration of 1 mg/mL was used as a sample.

<Manufacturing of fermented products>

After putting 1,000 parts by weight of 70% by weight of an ethanol aqueous solution in 100 parts by weight of the joiner, heating and extraction at 80° C. for 2 hours, the process of filtration with a filter paper having a size of 8 μm (whatman no. 2) is repeated twice to obtain an extract did The extract was concentrated under reduced pressure at 80 mmHg and 25° C. with a rotary vacuum evaporator to prepare a concentrate, and a medium containing the concentrate at 10 wt% was prepared at 121° C. and 1.5 atm for 15 minutes. Autoclaved and cooled to room temperature. Subsequently, lactic acid bacteria L. pentosus were subcultured in MRS broth and then inoculated with 1% (v/v), cultured for more than 48 hours in an incubator at 37° C., and fermented, 0.2 μm syringe filter. It was filtered and sterilized with a furnace to prepare a fermented material.

Then, each characteristic such as skin stability and anti-inflammation was measured by using the fermented fermented material as a sample through an experimental example to be described later. At this time, as a sample, a dilution prepared by mixing the fermented fermented material with distilled water at a concentration of 1 mg/mL was used.

[Comparative Example 1]

Using the fermented material of Example 2 as a sample, each characteristic such as skin stability and anti-inflammatory properties was measured through an experimental example to be described later. At this time, as a sample, a dilution prepared by mixing the fermented fermented fermented soybeans in distilled water at a concentration of 1 mg/mL was used.

[Experimental Example 1]

<Skin Stability Test>

Skin safety was evaluated for the anti-inflammatory peptide sample of Example 1, the anti-inflammatory composition sample of Example 2, and the fermented material sample of Comparative Example 1.

Specifically, a skin patch test was performed on 30 subjects (mean age 26 years, age distribution 19-38 years) using Haye's Test Chamber. At this time, patients with skin lesions such as psoriasis or eczema, pregnant, lactating women, or taking antihistamines were excluded from the experiment. The test site was washed with 70 wt% ethanol aqueous solution, dried, and 15 μg of each sample was dropped into the chamber, and then placed on the upper arm, which is the test site, and fixed. The patch was applied for 24 hours, and after removing the patch, the test site was marked with a marking pen, and the test site was observed after 0.5 hours, 24 hours and 48 hours, respectively. The determination was made according to the regulations of the International Contact Dermatitis Research Group (ICDRG) as shown in Table 1 below.

sign Criteria evaluation Average ± Suspicious or microreaction and erythema microstimulation 0 to 0.9 + Erythema + Cirrhosis mild stimulation 1.0 to 2.9 ++ Erythema + cirrhosis + vesicle central stimulus 3.0 to 4.9 +++ Erythema + Cirrhosis + blisters strong stimulus 5.0 or higher - no response non-irritant 0

As a result of the experiment, both the anti-inflammatory peptide sample of Example 1 and the anti-inflammatory composition sample of Example 2 had an average degree of irritation of 0, which was confirmed to be safe without irritation to the skin, and was also suitable for the skin of patients with atopic dermatitis.

[Experimental Example 2]

<Anti-inflammatory effect>

In order to evaluate the anti-inflammatory effect of the anti-inflammatory peptide sample of Example 1, the anti-inflammatory composition sample of Example 2, and the fermented fermented sample of Comparative Example 1, the nitric oxide production inhibitory ability of each sample was measured.

Nitric oxide is known as a chemical molecule that is produced in immune cells and regulates the immune system against external or internal harmful substances. Raw264.7 cells, a mouse-derived macrophage cell line, were cultured in a 12 well culture plate at a concentration of 5×10 ⁴ cells/well for 24 hours and treated with the sample for 2 hours, lipopolysaccharide (LPS) 0.1 μg/ ㎖ was treated and cultured for 24 hours. The culture solution was obtained, centrifuged at 3,000 rpm, 5 minutes, and 100 μl of the supernatant was mixed with 100 μl of griess reagent (mixed solution of 1% sulfanilamide and 0.1% N-(1-naphthyl)ethylene diamine dihydrochloride) to block light. was reacted for 15 minutes. The resulting reaction was measured at 450 nm absorbance with a microplate reader. In addition, the amount of nitric oxide produced was quantified using NaNO ₂ .

As a result, in Example 2, compared to Example 1 and Comparative Example 1, after LPS treatment, nitric oxide was inhibited at a significantly higher value compared to the control, confirming that there was a significant anti-inflammatory effect.

Hereinafter, a cosmetic preparation method including the anti-inflammatory peptide sample of Example 1 or the anti-inflammatory composition sample of Example 2 according to the present invention will be described through formulation examples. However, of course, the present invention is not limited thereto.

[Formulation Example 1]

<Production of lotion>

In purified water, 3% by weight of glycerin, 2% by weight of butylene glycol, 2% by weight of propylene glycol and 0.2% by weight of benzoin based on the total weight of the lotion were sequentially added to the reaction vessel and stirred to dissolve, and then polyoxyethylene hydrogenated castor oil 1% by weight was dissolved by heating to 60°C. Then, 2% by weight of the anti-inflammatory peptide sample of Example 1 or the anti-inflammatory composition sample of Example 2, 10% by weight of ethanol, and the remaining amount of purified water were added and thoroughly stirred, and then aged at 25° C. for 2 days to prepare a lotion, respectively.

[Formulation Example 2]

<Production of nourishing cream>

In purified water, 3 wt% of propylene glycol, 0.5 wt% of dextrin behenate and 0.2 wt% of benzoin were mixed and stirred at 80 °C with respect to the total weight of the nutritional cream. Then, using an emulsifier, beeswax 1% by weight, polysorbate 60 1.5% by weight, sorbitan sesquioleate 0.5% by weight, liquid paraffin 10% by weight, sorbitan stearate 1% by weight, lipophilic monostearate glycerin 0.5% by weight, 1.5% by weight of stearic acid and 1% by weight of glyceryl stearate were emulsified at 80°C. After the emulsification was completed, the mixture was cooled to 50°C while stirring using a stirrer. Then, 3% by weight of the anti-inflammatory peptide sample of Example 1 or the anti-inflammatory composition sample of Example 2 was added, cooled to 25° C., and aged at 25° C. for 2 days to prepare a nutritional cream, respectively. The content of the purified water corresponds to the remaining amount of the final prepared nutritional cream.

[Formulation Example 3]

<Nutrition Serum>

After mixing and stirring 0.02% by weight of xanthan gum, 10% by weight of butylene glycol, and 0.1% by weight of hydroxyethylcellulose based on the total weight of the nutritional serum at 40° C., using an emulsifier, 10% by weight of soybean phospholipid, sodium 5 wt% of hyaluronate and purified water were added to emulsify. After the emulsification was completed, the mixture was cooled to 35° C. while stirring using a stirrer, and 4% by weight of the anti-inflammatory peptide sample of Example 1 or the anti-inflammatory composition sample of Example 2 was respectively added. Then, after cooling to 25°C, the nutrient serum was prepared by aging at 25°C for 2 days. The content of the purified water corresponds to the remaining amount of the final prepared nutritional serum.

[Formulation Example 4]

<Production of Essence>

Sitosterol 1.7% by weight, polyethylene glycol 1.5% by weight, ceramide 0.7% by weight, stearic acid 1.2% by weight and cholesterol 1.5% by weight were homogenized at 25° C. to prepare a nonionic amphiphilic lipid mixture. After mixing and homogenizing 0.4% by weight of dicetylphosphate and 5% by weight of glycerin at 25° C. to the nonionic amphiphilic lipid mixture and passing it through a microfluidizer, 15% by weight of macadamia oil was slowly added at 55° C. and homogenized After that, it was passed through a microfluidizer again. Then, 2% by weight of the anti-inflammatory peptide sample of Example 1 or the anti-inflammatory composition sample of Example 2, 11% by weight of xanthan gum, 0.2% by weight of benzoin, and 0.3% by weight of carboxyvinyl polymer (weight average molecular weight: 1,200,000) Dispersion and stabilization and aged at 25° C. for 2 days to prepare an essence. The content of the purified water corresponds to the remaining amount of the final prepared essence.

Claims (11)

A cosmetic composition for improving atopic dermatitis comprising an anti-inflammatory peptide isolated from fermented beans. According to claim 1,
A cosmetic composition for improving atopic dermatitis, further comprising a fermented product obtained by fermenting an extract obtained by heat-extracting a member of the body. 3. The method of claim 2,
The pickled fermented product is
An extraction step of preparing an extract by heat-extracting the joiner with an extraction solvent containing ethanol; and
A cosmetic composition for improving atopic dermatitis produced by fermenting the extract with lactic acid bacteria. According to claim 1,
The anti-inflammatory peptide,
Fermentation step of fermenting a mixture containing legume raw materials and water
An extraction step of preparing a fermented extract obtained by extracting the fermented product obtained in the fermentation step with an extraction solvent, and
A cosmetic composition for improving atopic dermatitis, which is prepared including a separation step of isolating the anti-inflammatory peptide from the ferment extract. 5. The method of claim 4,
In the fermentation step, the mixture is a cosmetic composition for improving atopic dermatitis that is heat-treated at 70 to 150° C. of a mixture containing a legume raw material and water. 5. The method of claim 4,
In the fermentation step, the fermentation is performed at 30 to 45 ℃ cosmetic composition for improving atopic dermatitis. 5. The method of claim 4,
In the extraction step, the extraction solvent includes ethanol, and the extraction is a thermal extraction cosmetic composition for improving atopic dermatitis. 5. The method of claim 4,
The anti-inflammatory peptide,
between the extraction step and the separation step,
A cosmetic composition for improving atopic dermatitis prepared by further comprising the step of powdering the fermented extract. 9. The method of claim 8,
The separation step is a step of extracting and separating the anti-inflammatory peptide from the ferment extract powder obtained in the powdering step using a chromatography method, a cosmetic composition for improving atopic dermatitis. 5. The method of claim 4,
In the fermentation step, the legume raw material is a cosmetic composition for improving atopic dermatitis comprising soybeans. 11. An external preparation for skin comprising the cosmetic composition for improving atopic dermatitis according to any one of claims 1 to 10.

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