KR20220087273A - Method for preparing broth containing bean sprout extract and having the effect of relieving hangover and improving liver function, and composition thereof - Google Patents
Method for preparing broth containing bean sprout extract and having the effect of relieving hangover and improving liver function, and composition thereof Download PDFInfo
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- KR20220087273A KR20220087273A KR1020200177809A KR20200177809A KR20220087273A KR 20220087273 A KR20220087273 A KR 20220087273A KR 1020200177809 A KR1020200177809 A KR 1020200177809A KR 20200177809 A KR20200177809 A KR 20200177809A KR 20220087273 A KR20220087273 A KR 20220087273A
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- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 235000020234 walnut Nutrition 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
- 229940119569 wormwood extract Drugs 0.000 description 1
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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Abstract
본 발명은 알코올 섭취로 발생되는 숙취와 몸속에서 생성되는 알코올 독성을 신속하게 해소하기 위한 콩나물 추출물을 이용한 육수 조성물을 제공하기 위한 것이다. 이를 위해 본 발명은 정제수 100중량부에 부재료조성물 70~80중량부와 콩나물 35~40중량부를 혼합한 다음 90~110℃의 온도로 가열하여 콩나물 추출액을 추출한 다음 상기 콩나물추출액 100중량부에 하여 헛개나무농축액 1~2중량부를 혼합하여 제조한다. 상기 부재료조성물은 건다시마, 건표고버섯, 바지락살, 키조개관자 및 홍합살을 혼합하여 가열 추출하여 사용한다.본 발명은 숙취를 해소하고 과도한 음주로 발생되는 간 기능의 회복시켜 주며, 헛개나무농축액을 혼합하여 콩나물과 함께 육수조성물을 제조함으로써 헛개나무의 숙취해소 효과를 더욱 더 증가시켜 주는 효과가 있다. An object of the present invention is to provide a broth composition using a bean sprout extract for quickly resolving a hangover caused by alcohol ingestion and alcohol toxicity generated in the body. To this end, the present invention mixes 70-80 parts by weight of a sub-material composition and 35-40 parts by weight of bean sprouts with 100 parts by weight of purified water, and then extracts the bean sprouts extract by heating at a temperature of 90-110° C. It is prepared by mixing 1-2 parts by weight of the tree concentrate. The sub-ingredient composition is heated and extracted by mixing dried kelp, dried shiitake mushrooms, clam meat, clams and mussels. The present invention relieves a hangover and restores liver function caused by excessive drinking, and Heotgae tree concentrate Mixing and preparing a broth composition with bean sprouts has the effect of further increasing the hangover relieving effect of Heotgae tree.
Description
본 발명은 콩나물 추출물을 함유한 숙취해소와 간기능 개선 효능을 갖는 육수의 제조방법 및 그 조성물에 관한 것으로 더욱 상세하게는, 콩나물을 주원료로 하고 부원료로 헛개, 해초 등을 혼합하여 추출하고, 이를 이용한 숙취해소 및 간 장애 개선에 효능을 가지는 육수 조성물을 제조하는 방법에 관한 것이다.The present invention relates to a method and a composition for preparing broth containing bean sprouts extract having the effect of relieving hangover and improving liver function, and more particularly, to extract by mixing bean sprouts as a main raw material, and seaweed as sub-materials, and extracting it It relates to a method for preparing a broth composition having efficacy in relieving hangover and improving liver disorders.
일반적으로 알코올은 식품이나 약품으로 사용되며 전 세계적으로 인류와 밀접한 관계가 있는 기호식품의 하나이며, 국내에서는 1980년 이후 경제가 성장함에 따라 알코올 소비량 또한 증가하여 세계에서 알코올 섭취가 많은 국가 중 하나로 분류되고 있다.In general, alcohol is used as food or medicine, and it is one of the favorite foods that are closely related to mankind around the world. is becoming
이에 국내에서는 알코올 섭취시 발생하는 간질환 역시 증가하고 있으며, 알코올성 간질환을 예방하는 연구에 대한 관심이 높아지고 있다.Accordingly, liver disease caused by alcohol consumption is also increasing in Korea, and interest in research to prevent alcoholic liver disease is increasing.
알코올의 분해과정은 알코올(alcohol)이 체내로 흡수되면 알코올 탈수소효소(alcohol dehydrogenase)에 의해 아세트알데히드(acetaldehyde)로 분해되며, 이는 아세트알데히드 탈수소효소(acetaldehyde dehydrogenase)로 인해 산화되어 아세트산(acetic acid)로 대사되는데 그 중 일부는 소변, 땀 및 호흡 등을 통해 체외로 배출된다.In the decomposition process of alcohol, when alcohol is absorbed into the body, it is decomposed into acetaldehyde by alcohol dehydrogenase, which is oxidized by acetaldehyde dehydrogenase to acetic acid. Some of it is excreted from the body through urine, sweat and respiration.
체내에 알코올을 과도하게 섭취하는 경우 알코올 분해과정에서 독성이 강한 아세트알데히드가 생성되며, 이 아세트알데히드가 체내에 축적됨으로써 활성산소종이 증가하여 숙취해소 및 대사기능에 장애를 일으키고, 이로 인해 지방산 및 중성지방 합성의 증가로 알코올성 지방간, 알코올성 간염과 같은 간 질환 등이 유발된다.When alcohol is consumed excessively in the body, acetaldehyde, which is highly toxic, is generated during the process of alcohol decomposition, and this acetaldehyde accumulates in the body, thereby increasing reactive oxygen species, causing hangover relieving and metabolic dysfunction. The increase in fat synthesis leads to liver diseases such as alcoholic fatty liver and alcoholic hepatitis.
이러한 문제를 해결하기 위해 종래기술로 국내등록특허 제10-0718189호가 제시된 바 있다.In order to solve this problem, Korean Patent Registration No. 10-0718189 has been proposed as a prior art.
상기 종래기술은 숙취해소 및 간기능 회복에 효과가 있는 천연차 및 그 제조방법에 관한 것으로 아카시아 나무(Robinia pseudo-acacia)의 잎, 줄기, 꽃, 뿌리의 추출물을 주성분으로 하고 여기에 통상의 부가제가 첨가되어 구성될 수 있고, 여기에 부가적으로, 백화사설초(白花蛇舌草), 사인, 감초, 또는 갈화(葛花)와 같은 것을 부원료로 부가되어 구성됨을 특징으로 하는 발명이다.The prior art relates to a natural tea effective in relieving a hangover and recovery of liver function, and a method for manufacturing the same. It is an invention characterized in that it can be constituted by adding an agent, and additionally, white flower saseolcho (白花蛇舌草), coriander, licorice, or brown flower (葛花) is added as an auxiliary material.
하지만 상기 종래기술은 아카시아나무를 활용하는 것으로 숙취해소 능력이 크지 않고, 더구나 간 기능 개선 효과가 크지 않은 문제점이 있다. However, in the prior art, there is a problem that the hangover relieving ability is not large by using an acacia tree, and the effect of improving liver function is not large.
본 발명은 상기와 같은 문제점을 해결하기 위한 것으로 그 목적은 알코올 섭취로 발생되는 숙취와 몸속에서 생성되는 알코올 독성을 신속하게 해소하기 위한 콩나물 추출물을 이용한 육수 조성물을 제공하기 위한 것이다. The present invention is to solve the above problems, and an object of the present invention is to provide a broth composition using a bean sprout extract for quickly resolving a hangover caused by alcohol ingestion and alcohol toxicity generated in the body.
또한 본 발명은 알코올의 섭취로 인하여 발생되는 간 기능의 저하를 막고 간기능 장애를 회복시키기 위한 콩나물 육수 조성물을 제공하기 위한 것이다.Another object of the present invention is to provide a bean sprouts broth composition for preventing a decrease in liver function caused by ingestion of alcohol and recovering a liver dysfunction.
또한, 상기 육수를 이용하여 간편식(HMR)으로 제조하여 접근성을 높이는 것을 목적으로 한다.In addition, it aims to increase accessibility by manufacturing it as a simple meal (HMR) using the broth.
본 발명의 콩나물 추출물을 함유한 숙취해소와 간기능 개선 효능을 갖는 육수의 제조방법은 정제수 100중량부에 부재료조성물 70~80중량부와 콩나물 35~40중량부를 혼합한 다음 90~110℃의 온도로 가열하여 콩나물 추출액을 추출한 다음 상기 콩나물추출액 100중량부에 하여 헛개나무농축액 1~2중량부를 혼합하여 제조한다. 상기 부재료조성물은 건다시마, 건표고버섯, 바지락살, 키조개관자 및 홍합살을 혼합하여 가열 추출하여 사용한다.The method for producing broth containing bean sprout extract of the present invention having the effect of relieving hangover and improving liver function is mixing 70 to 80 parts by weight of a sub-ingredient composition and 35 to 40 parts by weight of bean sprouts with 100 parts by weight of purified water, followed by a temperature of 90 to 110 ° C. After extracting the bean sprouts extract by heating with 100 parts by weight of the bean sprouts extract, it is prepared by mixing 1 to 2 parts by weight of the Heotgae tree concentrate. The auxiliary material composition is used by heating and extracting dried kelp, dried shiitake mushrooms, clam meat, clams and mussels by mixing them.
상기 부재료조성물에 바지락살, 키조개관자 및 홍합살을 투입하는 경우 상기 바지락살 및 키조개관자, 홍합살을 하나 또는 둘 이상을 혼합한 다음 100℃ 내지 110℃에서 2시간 동안 추출하고, 여기에 다시 대파, 무, 양파 및 소금을 투입하여 90℃ 내지 102℃에서 35분간 추출하여 콩나무추출액을 제조한다.When adding clams, clams, and mussels to the sub-material composition, one or two or more of the clams, clams, and mussels are mixed, and then extracted at 100° C. to 110° C. for 2 hours, and here again green onions , radish, onion and salt are added and extracted at 90°C to 102°C for 35 minutes to prepare a bean extract.
상기 콩나물추출액을 제조할 때 콩나물을 투입하여 온도 100℃ 내지 110℃에서 20분간 추출과정을 거치고, 고추씨를 투입하여 90℃ 내지 100℃에서 다시 20분간 추출한 후 이를 여과하여 콩나물추출물을 제조한다.When preparing the bean sprouts extract, the bean sprouts are put through an extraction process at a temperature of 100°C to 110°C for 20 minutes, red pepper seeds are added and extracted again at 90°C to 100°C for 20 minutes, and then filtered to prepare a bean sprouts extract.
본 발명으로 제조된 나물 추출물을 함유한 숙취해소와 간기능 개선 효능을 갖는 육수조성물은 알코올 섭취의 증가로 발생하는 숙취를 해소시켜 주는 효과를 제공한다. 또한 과도한 음주로 발생되는 간 기능의 회복하여 준다.The broth composition having the effect of relieving hangover and improving liver function containing the herb extract prepared by the present invention provides an effect of relieving hangover caused by an increase in alcohol intake. It also restores liver function caused by excessive drinking.
또한 본 발명은 헛개나무농축액을 혼합하여 콩나물과 함께 육수조성물을 제조함으로써 헛개나무의 숙취해소 효과를 더욱 더 증가시켜 주는 효과가 있다.In addition, the present invention has the effect of further increasing the hangover relieving effect of hutgae tree by preparing a broth composition with bean sprouts by mixing heotgae tree concentrate.
또한 본 발명은 해산물인 키조개관자, 홍합살 및 바지락살을 함께 사용하여 맛과 영양을 함께 제공하여 소비자의 입맛에 맛는 숙취해소 육수 조성물을 제공할 수 있다.In addition, the present invention can provide a hangover-relieving broth composition that tastes good to consumers' tastes by using seafood, such as clams, mussels, and clam meat together to provide both taste and nutrition.
이하, 본 발명에 따른 콩나물 추출물을 함유한 숙취해소와 간기능 개선 효능을 갖는 육수의 제조방법 및 그 조성물에 대하여 실시예를 참조하여 상세하게 설명하도록 한다. 본 발명은 이하에서 개시되는 실시예에 한정되는 것이 아니라, 서로 다른 다양한 형태로 구현될 수 있으며, 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자에게 발명의 범주를 완전하게 알려주기 위해 제공되는 것이다. 또한, 본 발명이 첨부된 특허청구의 범위에 속하는 모든 실시 태양을 포함하는 것으로 해석되어야 한다.Hereinafter, a method for preparing a broth containing bean sprout extract and having an effect of relieving a hangover and improving liver function according to the present invention and a composition thereof will be described in detail with reference to Examples. The present invention is not limited to the embodiments disclosed below, but may be implemented in a variety of different forms, and is provided to completely inform those of ordinary skill in the art to which the present invention pertains to the scope of the invention. will be. Further, it should be construed that the present invention includes all embodiments falling within the scope of the appended claims.
본 발명은 콩나물 추출물을 함유한 숙취해소와 간기능 개선 효능을 갖는 육수성물의 제조방법에 관한 것이다. 본 발명의 육수조성물은 물 100중량부에 건다시마와 건표고버섯을 포함하는 부재료혼합물을 70~80중량부를 혼합하고, 상기 90~105℃의 온도에서 1~2분 동안 가열 추출하여 부재료조성물을 제조한다. 상기 부재료혼합물에 사용되는 건다시마는 0.1~1중량부를 넣고 건표고버섯은 0.1~5중량부를 혼합하여 끓는 온도에서 1~2분 동안 가열하여 다시마추출액을 제조한다.The present invention relates to a method for producing a broth containing a bean sprout extract having the effect of relieving a hangover and improving liver function. The broth composition of the present invention is obtained by mixing 70 to 80 parts by weight of a sub-ingredient mixture containing dried kelp and dried shiitake mushrooms in 100 parts by weight of water, and extracting the sub-ingredient composition by heating at a temperature of 90 to 105° C. for 1 to 2 minutes. manufacture 0.1 to 1 parts by weight of dried kelp used in the auxiliary material mixture is added, and 0.1 to 5 parts by weight of dried shiitake mushrooms are mixed and heated at a boiling temperature for 1 to 2 minutes to prepare a kelp extract.
상기와 같이 건다시마는 육수조성물의 깊은 맛을 제공할 뿐만 아니라 조미 역할도 하며, 건표고버섯은 표고버섯의 영양성분과 함께 건다시마의 맛에 더해 육수조성물의 맛을 깔끔하게 해 준다.As described above, dried kelp not only provides a deep taste of the broth composition, but also serves as a seasoning.
상기 다시마추출액에 해산물을 포함하여 해산물다시마추출액을 제조함으로써 해산물의 깊은 맛과 영양을 제공하여 육수조성물의 숙취해소 효과를 촉진시키며, 간 기능을 좋게 하기 위한 영양 성분을 제공한다. 상기 해산물에는 바지락살 30~40중량부, 키조개관자 5~10중량부 및/또는 홍합살 5~10중량부를 더 혼합하여 사용되며, 상기 해산물을 투입한 다음 100~110℃의 온도에서 100~200분 동안 가열하여 추출한다.By preparing a seafood kelp extract including seafood in the kelp extract, it provides the deep taste and nutrition of seafood to promote the hangover relieving effect of the broth composition, and provides nutrients for improving liver function. The seafood is used by further mixing 30-40 parts by weight of clam meat, 5-10 parts by weight of clams, and/or 5-10 parts by weight of mussels, 100-200 at a temperature of 100-110° C. after adding the seafood. Extract by heating for minutes.
상기 해산물다시마추출액에 콩나물 35~40중량부를 혼합하여 콩나물의 숙취기능을 가미한 육수조성물을 제조한다. 상기 해산물다시마추출액에 콩나물을 투입한 다음 100~110℃의 온도로 가열하여 콩나물추출액을 제조한다.35-40 parts by weight of bean sprouts are mixed with the seafood kelp extract to prepare a broth composition adding the hangover function of bean sprouts. Bean sprouts are added to the seafood kelp extract and then heated to a temperature of 100 to 110° C. to prepare a bean sprouts extract.
그런 다음 상기 콩나물추출액 100중량부에 헛개나무농축액 1~2중량부를 혼합하여 콩나물 육수를 제조한다. 상기 헛개나무 농축액은 잘 건조된 헛개나무를 물 100중량부에 5~50중량부를 혼합한 다음 100~120℃의 온도에서 100~300분 가열하여 열수추출한 농축액을 사용한다.Then, the bean sprouts broth is prepared by mixing 1-2 parts by weight of the Heotgae tree concentrate with 100 parts by weight of the bean sprouts extract. The hutgae tree concentrate is a concentrated solution obtained by mixing 5-50 parts by weight of dried huotgae tree with 100 parts by weight of water and then heating it for 100-300 minutes at a temperature of 100-120° C. and hot water extraction.
헛개나무 농축액 또는 추출액은 아세트알데히드의 분해를 촉진하여 과음으로 발생되는 숙취를 빠르게 회복시켜 주며, 헛개나무에 많이 들어 있는 오메가3 지방산과 여러 아미노산 성분은 간 기능을 개선시켜 준다. 즉, 간 기능 저하를 유발하는 독소를 배출시켜 간세포 활성화에 도움을 준다. 그 외에 이뇨작용 촉진, 관절염 개는 및 스트레스 완화에 도움을 준다.Hedgehog concentrate or extract promotes the decomposition of acetaldehyde to quickly recover a hangover caused by excessive drinking. In other words, it helps to activate hepatocytes by releasing toxins that cause a decrease in liver function. In addition, it promotes diuresis, helps arthritis dogs and relieves stress.
상기 콩나물 추출액을 제조할 때 부재료혼합물에 고추씨를 혼합하여 육수조성물의 맛을 개운하게 해줄 수 있으며, 이때 고추씨는 1~5중량부를 혼합하는 것이 바람직하다. 상기 고추씨를 콩나무추출액에 혼합한 다음 90내지 100℃의 온도에서 10~30분 동안 가열하는 것이 바람직하다.When preparing the bean sprouts extract, red pepper seeds are mixed with the auxiliary material mixture to improve the taste of the broth composition, and in this case, it is preferable to mix 1 to 5 parts by weight of red pepper seeds. It is preferable to mix the red pepper seeds with the soybean extract and then heat at a temperature of 90 to 100° C. for 10 to 30 minutes.
상기 콩나물 추출액을 제조할 때 부재료혼합물에 조미원료를 더 포함하여 맛을 증진시켜 육수조성물의 깊은 맛을 증가시킨다. 상기 조미원료는 대파 3~10중량부, 무 2~8중량부, 양파 2~6중량부 및/또는 소금 0.5 내지 2중량부를 상기 콩나무추출액에 혼합한 다음 98~120℃의 온도에서 20~40분 동안 가열하여 준다.When preparing the bean sprouts extract, the flavor is enhanced by further including seasoning raw materials in the sub-ingredient mixture to increase the deep taste of the broth composition. The seasoning raw material is mixed with 3 to 10 parts by weight of green onion, 2 to 8 parts by weight of radish, 2 to 6 parts by weight of onion and/or 0.5 to 2 parts by weight of salt to the bean extract, and then 20 to 40 at a temperature of 98 to 120 ° C. Heat for a minute.
1. 부재료 혼합물의 조성1. Composition of the sub-material mixture
본 발명의 콩나물 추출액에 사용되는 부재료혼합물의 조성은 일 실시예로서 상기의 조성과 같이 조성할 수 있다.The composition of the auxiliary material mixture used in the bean sprouts extract of the present invention may be the same as the composition described above as an embodiment.
2. 콩나물추출물의 조성2. Composition of bean sprouts extract
본 발명의 콩나물추출물의 조성은 일 실시예로 상기와 같은 비율로 조성할 수 있다.The composition of the bean sprouts extract of the present invention may be formulated in the same ratio as described above in one embodiment.
가. 관능적 기호성 평가를 통한 콩나물·헛개 혼합물의 최적 조건 확립go. Establishment of optimal conditions for bean sprouts/hutgae mixture through sensory palatability evaluation
콩나물 추출물(BSM)에 헛개 농축액을 다양한 농도로 첨가하여 콩나물·헛개 혼합물(BH)의 조건을 확립하고자 냄새 및 맛의 전반적인 기호도를 확인하였다. 헛개 추출물의 관능검사는 색깔, 단맛, 이취, 풀 맛, 비린 맛 및 전체적인 기호도를 포함한 6가지 항목으로 평가되었으며, 선정된 관능요원으로는 충분한 훈련을 거쳐 품질차이를 식별할 수 있는 능력이 갖추어진 20대 대학생 20명을 선별하여 실시하였다. In order to establish the conditions of the bean sprouts mixture (BH) by adding various concentrations of the bean sprouts extract (BSM), the overall palatability of smell and taste was confirmed. The sensory test of H. wormwood extract was evaluated with six items including color, sweetness, off-flavor, grassy taste, fishy taste, and overall preference. 20 college students in their 20s were selected and conducted.
3. 관능평가를 통한 헛개 추출물의 함량 결정3. Determination of the content of Heotgae extract through sensory evaluation
(최적 콩나물 추출물의 이화학적 특성 분석) (Analysis of physicochemical properties of optimal bean sprout extract)
1) 재료 및 방법 1) Materials and methods
가. HPLC 분석 go. HPLC analysis
HPLC 분석은 HPLC-PDA (Shinmadzu Inc., Walnut Creek, CA, USA)를 이용하여 진행하였으며, 시료를 0.45μm membrane filter 여과한 후 분석하였고, 지표물질 ampelopsin은 ChemFaces에서 구매하여 사용하였다. (ChemFaces Biochemical Co., Ltd. Wuhan, China) 분석조건은 아래와 같다. HPLC analysis was performed using HPLC-PDA (Shinmadzu Inc., Walnut Creek, CA, USA), and the sample was analyzed after filtration with a 0.45 μm membrane filter, and the indicator material ampelopsin was purchased from ChemFaces and used. (ChemFaces Biochemical Co., Ltd. Wuhan, China) The analysis conditions are as follows.
4. HPLC 분석 조건4. HPLC analysis conditions
A (0.05% phophoric acid)
B (ACN)
time (min) A B
0 ~ 4 95% 5%
4 ~ 10 95 ~ 50 5 ~ 50%
10 ~ 20 50 ~ 10% 50 ~ 90%
20 ~ 22 10 ~ 50% 90 ~ 50%
22 ~ 23 50 ~ 95% 50 ~ 5%
30 95% 5%
A (0.05% phophoric acid)
B (ACN)
time (min) AB
0 to 4 95% 5%
4 to 10 95 to 50 5 to 50%
10 to 20 50 to 10% 50 to 90%
20 ~ 22 10 ~ 50% 90 ~ 50%
22 ~ 23 50 ~ 95% 50 ~ 5%
30 95% 5%
나. 유리당 및 유기산 분석 me. Analysis of free sugars and organic acids
5. 유리당 및 유기산 분석 조건5. Free Sugar and Organic Acid Analysis Conditions
(250x4.6mm I.D)InertsilODS-3V
(250x4.6mm ID)
(4 ×250 mm)CarboPAC TM-PA10
(4 × 250 mm)
다. 유리아미노산 분석All. Free Amino Acid Analysis
유리아미노산 분석은 시료 10 mL에 sulfosalicylic acid 25 mg을 첨가하여 4°에서 4시간 동안 방치시킨 후 원심분리(50,000 rpm, 30분)하여 단백질 등을 제거 하고, 상등액을 0.22μm membrane filter로 여과하여 얻은 여액을 취하여 분석시료로 사용하였으며 분석조건은 아래와 같다. For free amino acid analysis, 25 mg of sulfosalicylic acid was added to 10 mL of sample, left for 4 hours at 4°, centrifuged (50,000 rpm, 30 minutes) to remove proteins, and the supernatant was filtered with a 0.22 μm membrane filter. The filtrate was taken and used as an analysis sample, and the analysis conditions are as follows.
6. 유리아미노산 분석 조건6. Free Amino Acid Analysis Conditions
(11 μm ±2 μm, 200 mm)Ultrapac 11 cation exchange resin
(11 μm ±2 μm, 200 mm)
라. 무기성분 분석 la. Inorganic component analysis
무기성분 분석은 습식분해법으로 행하였다. 시료용액 100 mL에 분해제(HCIO4 : H2SO4 : H2O2 = 9 : 2 : 5, v/v) 25 mL를 가하여 낮은 온도에서 서서히 가열하여 완전하게 무색으로 변할 때까지 hot plate에서 분해한 후 여과 (Whatman NO.2)하여 100 mL 정용하였으며, 이를 시료로 하여 atomic absorption spectrometer을 사용해서 아래와 같은 조건으로 분석하였다. Inorganic component analysis was performed by the wet decomposition method. Add 25 mL of decomposing agent (HCIO4: H2SO4: H2O2 = 9: 2: 5, v/v) to 100 mL of sample solution, heat slowly at low temperature, decompose on a hot plate until completely colorless, and filter (Whatman No.2) was used and 100 mL was used, and the sample was analyzed under the following conditions using an atomic absorption spectrometer.
7. 무기성분 분석 조건7. Conditions for Inorganic Analysis
마. 총 폴리페놀 함량 측정 mind. Determination of total polyphenol content
Folin-Ciocalten (Gao, X., Bjork, L., Trajkovski, V. and Uggla, M. 2000)방법에 따라 총 폴리페놀 함량을 측정하였다. 시료 0.1 ml에 증류수 8.4 ml 및 2 N Folin-Ciocalten 시약(Sigma-Aldrich, Co., ST. Louis, MO, USA) 0.5 ml과 20% Na2CO3 (Junsei Chemical Co., Ltd, Japan) 1 ml을 첨가한 후 2시간 동안 방치하였다. 모든 시약을 첨가한 시료의 흡광도는 UV/Vis-spectrophotometer (U-1800, Hitachi)를 사용하여 725 nm에서 측정되었으며, gallic acid (Sigma-Aldrich, Co.)를 표준곡선으로 이용하여 양을 환산하였다.Total polyphenol content was measured according to the Folin-Ciocalten (Gao, X., Bjork, L., Trajkovski, V. and Uggla, M. 2000) method. To 0.1 ml of sample, 8.4 ml of distilled water and 0.5 ml of 2 N Folin-Ciocalten reagent (Sigma-Aldrich, Co., ST. Louis, MO, USA) and 20% Na 2 CO 3 (Junsei Chemical Co., Ltd, Japan) 1 ml was added and left for 2 hours. The absorbance of the samples to which all reagents were added was measured at 725 nm using a UV/Vis-spectrophotometer (U-1800, Hitachi), and the amount was converted using gallic acid (Sigma-Aldrich, Co.) as a standard curve. .
바. 총 플라보노이드 함량 측정 bar. Determination of total flavonoid content
(Abdel-Hameed, E. S. S. 2009)의 방법을 변형시켜 총 플라보노이드 함량을 측정하였다. 시료 1 ml에 5% sodium nitrite 0.15 ml을 첨가하여 25℃에서 6분간 반응시킨 후 10% aluminum chloride 0.3 ml을 넣고 5분 동안 방치하였다. 그 후 1 N NaOH 1 ml을 가하고 교반한 후 흡광도를 510 nm에서 측정하였고, rutin hydrate (Sigma-Aldrich Co.)를 표준곡선으로 이용하여 양을 환산하였다. (Abdel-Hameed, E. S. S. 2009) was modified to determine the total flavonoid content. 0.15 ml of 5% sodium nitrite was added to 1 ml of the sample, and after reaction at 25° C. for 6 minutes, 0.3 ml of 10% aluminum chloride was added and left for 5 minutes. After that, 1 ml of 1 N NaOH was added and stirred. The absorbance was measured at 510 nm, and the amount was converted using rutin hydrate (Sigma-Aldrich Co.) as a standard curve.
(최적 콩나물 추출물의 이화학적 특성 분석) (Analysis of physicochemical properties of optimal bean sprout extract)
2) 결과 및 고찰 2) Results and considerations
가. HPLC 분석 go. HPLC analysis
8. Ampelopsin 함량 분석8. Ampelopsin content analysis
1)BSM: Bean sprout extract 1) BSM: Bean sprout extract
2)HDE: 1.5% Hovenia dulcis extract 2) HDE: 1.5% Hovenia dulcis extract
3)BH: Bean sprout·Hovenia dulcis mixture 3) BH: Bean sprout · Hovenia dulcis mixture
각 시료들의 ampelopsin 함량을 분석한 결과는 위의 표와 같다. BSM, HDE 및 BH에서 각각 1.947 ppm, 10.218 ppm, 10.524 ppm으로 헛개가 함유 된 HDE 및 BH의 ampelopsin 함량이 헛개를 함유하지 않은 BSM 보다 풍부하였으며, 그 중 BH에서 가장 높은 함량을 확인 할 수 있었다. The results of analyzing the ampelopsin content of each sample are shown in the table above. In BSM, HDE, and BH, the ampelopsin contents of HDE and BH were richer than those of BSM without sorghum at 1.947 ppm, 10.218 ppm, and 10.524 ppm, respectively, and the highest content was confirmed in BH.
가. 유리당 go. free sugar
9. BSM, HDE, BH의 유리당 함량 변화9. Changes in free sugar content of BSM, HDE, and BH
1)BSM: Bean sprout extract 1) BSM: Bean sprout extract
2)HDE: 1.5% Hovenia dulcis extract 2) HDE: 1.5% Hovenia dulcis extract
3)BH: Bean sprout·Hovenia dulcis mixture 3) BH: Bean sprout · Hovenia dulcis mixture
각 시료들의 유리당 함량 변화를 측정한 결과는 위의 표와 같다. BSM에서 sucrose는 60 ppm이였으며, glucose는 215 ppm, fructose는 257 ppm이였다. HDE에서는 sucorse가 294 ppm, glucose는 368 ppm, fructose는 432 ppm이였다. BH에서의 sucorse, glucose 및 fructose는 375 ppm, 645 ppm 및 738 ppm으로 나타났다. The results of measuring the change in the free sugar content of each sample are shown in the table above. In BSM, sucrose was 60 ppm, glucose was 215 ppm, and fructose was 257 ppm. In HDE, sucorse was 294 ppm, glucose was 368 ppm, and fructose was 432 ppm. The concentrations of sucorse, glucose and fructose in BH were 375 ppm, 645 ppm and 738 ppm.
나. 유기산 me. organic acid
10. BSM, HDE, BH의 유리산 함량 변화10. Changes in free acid content of BSM, HDE, and BH
1)BSM: Bean sprout extract 1) BSM: Bean sprout extract
2)HDE: 1.5% Hovenia dulcis extract 2) HDE: 1.5% Hovenia dulcis extract
3)BH: Bean sprout·Hovenia dulcis mixture 3) BH: Bean sprout · Hovenia dulcis mixture
4)ND: Not detected 4) ND: Not detected
각 시료들의 유리당 함량 변화를 측정한 결과는 위의 표와 같다. 검출된 주요 유기산인 malic acid는 식품의 풍미를 향상시키는 것으로 알려져 있으며 BSM, HDE 및 BH에서 각각 559 ppm, 919 ppm 및 1451 ppm으로 분석되었고, BH에서 가장 많은 함량이 검출 되었다. The results of measuring the change in the free sugar content of each sample are shown in the table above. Malic acid, the main organic acid detected, is known to improve the flavor of food and was analyzed at 559 ppm, 919 ppm and 1451 ppm in BSM, HDE and BH, respectively, and the highest content was detected in BH.
다. 유리아미노산 All. free amino acids
11. BSM, HDE, BH의 유리아미노산 함량 변화11. Changes in free amino acid content of BSM, HDE, and BH
1)BSM: Bean sprout extract 1) BSM: Bean sprout extract
2)HDE: 1.5% Hovenia dulcis extract 2) HDE: 1.5% Hovenia dulcis extract
3)BH: Bean sprout·Hovenia dulcis mixture 3) BH: Bean sprout · Hovenia dulcis mixture
4)ND: Not detected 4) ND: Not detected
BSM에서 가장 많이 검출 된 유리아미노산 두 종은 taurine과 arginine이며, HDE는 glutamic acid 및 alanine, BH는 taurine 및 arginine으로 나타났다. 또한 BSM, HDE 및 BH의 총 유리아미노산 함량은 각각 97.74 ㎍/ml, 2.63 ㎍/ml 및 92.05 ㎍/ml이 검출되었다. 이렇게 시료마다 검출되는 아미노산의 함량이 다른 이유로는 시료의 화학적 조성 차이나 이에 용출되는 유리아미노산의 함량 및 종류의 차이에 의해 각 시료마다 검출되는 유리아미노산의 함량에 변화가 나타난다고 보고된 바와 같이 본 연구에서도 이와 같은 원인 등으로 인해 BSM, HDE 및 BH 각각의 유리아미노산 함량에 차이가 나타났다. The two free amino acids most detected in BSM were taurine and arginine, glutamic acid and alanine in HDE, and taurine and arginine in BH. In addition, the total free amino acid contents of BSM, HDE and BH were detected to be 97.74 μg/ml, 2.63 μg/ml and 92.05 μg/ml, respectively. As reported in this study, the reason for the difference in the content of amino acids detected in each sample is that the content of free amino acids detected in each sample changes due to differences in the chemical composition of the samples or the content and type of free amino acids eluted therefrom. Also, there was a difference in the free amino acid content of each of BSM, HDE, and BH due to these causes.
라. 무기질la. minerals
12. BSM, HDE, BH의 무기성분 함량 변화12. Changes in the content of inorganic components of BSM, HDE, and BH
1)BSM: Bean sprout extract 1) BSM: Bean sprout extract
2)HDE: 1.5% Hovenia dulcis extract 2) HDE: 1.5% Hovenia dulcis extract
3)BH: Bean sprout·Hovenia dulcis mixture 3) BH: Bean sprout · Hovenia dulcis mixture
4)ND: Not detected 4) ND: Not detected
각 시료들의 무기성분 함량을 분석한 결과는 위의 표와 같다. 칼륨(K), 마그네슘(Mg), 나트륨(Na), 칼슘(Ca), 철(Fe), 망간(Mn), 아연(Zn), 구리(Cu)와 같이 총 8가지의 무기질을 검출하였다. 그 결과 BSM, HDE 및 BH는 미네랄 함량 중 Zn, Fe, Mn에서 가장 높은 값을 나타냈으며, 특히 BH는 Fe 및 Mn에서 20 ppm 및 2.84 ppm으로 BSM과 HDE 보다 높은 값을 나타냈다. The results of analyzing the inorganic component content of each sample are shown in the table above. A total of eight minerals were detected, such as potassium (K), magnesium (Mg), sodium (Na), calcium (Ca), iron (Fe), manganese (Mn), zinc (Zn), and copper (Cu). As a result, BSM, HDE, and BH showed the highest values in Zn, Fe, and Mn among mineral contents, and in particular, BH showed higher values than BSM and HDE at 20 ppm and 2.84 ppm in Fe and Mn.
마. 총 폴리페놀 함량 mind. Total polyphenol content
13. BSM, HDE, BH의 총 폴리페놀 함량 측정 13. Determination of total polyphenol content of BSM, HDE and BH
1)GAE: Galic acid equivalent 1) GAE: Galic acid equivalent
2)BSM: Bean sprout extract 2) BSM: Bean sprout extract
3)HDE: 1.5% Hovenia dulcis extract 3) HDE: 1.5% Hovenia dulcis extract
4)BH: Bean sprout·Hovenia dulcis mixture 4) BH: Bean sprout · Hovenia dulcis mixture
총 폴리페놀 화합물의 함량은 957.1 ㎍/ml로 가장 높은 함량을 보였으며, BSM과 HDE의 함량은 각각 531 ㎍/ml, 500.6 ㎍/ml로 BH와 유의적인 차이를 나타냈다. The content of total polyphenol compounds was 957.1 μg/ml, showing the highest content, and the contents of BSM and HDE were 531 μg/ml and 500.6 μg/ml, respectively, indicating a significant difference from BH.
바. 총 플라보노이드 함량 bar. Total flavonoid content
14. BSM, HDE, BH의 총 플라보노이드 함량 측정14. Determination of total flavonoid content of BSM, HDE, BH
1)QE: Quercetin equivalent 1) QE: Quercetin equivalent
2)BSM: Bean sprout extract 2) BSM: Bean sprout extract
3)HDE: 1.5% Hovenia dulcis extract 3) HDE: 1.5% Hovenia dulcis extract
4)BH: Bean sprout·Hovenia dulcis mixture 4) BH: Bean sprout · Hovenia dulcis mixture
총 플라보노이드 함량은 BH가 601.93 ㎍/ml으로 높게 나타났으며, BSM은 373 ㎍/ml 및 HDE는 367 ㎍/ml로 위의 폴리페놀의 함량과 유사한 경향을 나타냈다. The total flavonoid content was as high as 601.93 μg/ml for BH, 373 μg/ml for BSM and 367 μg/ml for HDE, showing a similar trend to the polyphenol content above.
(콩나물·헛개 혼합물의 항산화 활성 검증)(Verification of antioxidant activity of bean sprouts/Hunggae mixture)
1) 재료 및 방법 1) Materials and methods
가. DPPH radical 소거활성 go. DPPH radical scavenging activity
각 시료의 DPPH radical 소거활성은 (Blois, M. S. 1958)의 방법에 따라 측정하였다. α,α'-diphenyl-β-picrylhydrazine (DPPH)의 환원성을 이용하여 517 nm의 흡광도로 측정되었다. 각각의 시료들 및 대조구로 사용한 0.1%α-tocopherol과 0.1% dibutylated hydroxytoluene (BHT) 1 ml를 0. 4 mM DPPH 용액 3 ml과 5초 동안 vortex mixer로 혼합하고 이를 30분간 암소에서 반응시켜 흡광도를 측정하였다. Control은 에탄올 1 ml을 시료대신 첨가하여 control에 대한 흡광도의 감소비율로 나타냈다. The DPPH radical scavenging activity of each sample was measured according to the method (Blois, M. S. 1958). The absorbance was measured at 517 nm using the reducing property of α,α'-diphenyl-β-picrylhydrazine (DPPH). For each sample and control, 0.1% α-tocopherol and 1 ml of 0.1% dibutylated hydroxytoluene (BHT) were mixed with 3 ml of a 0.4 mM DPPH solution with a vortex mixer for 5 seconds, and reacted in a dark place for 30 minutes to measure absorbance measured. Control was expressed as the ratio of decrease in absorbance to control by adding 1 ml of ethanol instead of the sample.
나. ABTS· radical scavenging activityme. ABTS radical scavenging activity
(Biglari, F., AIKarkhi, A. M. F. 2008)의 방법으로 각 시료들에 대한 ABTS radical 소거 활성을 측정하였다. 7 mM의 2,2'-Azobi(2-aminopropane) dihydrochloride (Sigma-Aldrich Co.)는 2.45 mM의 ABTS와 혼합하여 16시간 동안 23℃의 암소에서 반응시켰다. ABTS 용액의 농도는 734 nm의 흡광도에서 측정되었으며, 0.70*?*의 흡광도가 되도록 증류수를 이용하여 조정하였다. 시료 0.1 ml과 3.9 ml의 ABTS 용액을 혼합하고 vortexing 한 후 실온에서 6분간 반응시켰다. 반응이 끝난 후 UV/Vis-spectrophotometer (U-1800, Hitachi)로 734 nm에서 흡광도를 측정하였다. ABTS radical 소거활성은 control을 기준으로 흡광도의 감소비율(%)로 표시하였다. (Biglari, F., AIKarkhi, A. M. F. 2008) ABTS radical scavenging activity for each sample was measured. 7 mM 2,2'-Azobi(2-aminopropane) dihydrochloride (Sigma-Aldrich Co.) was mixed with 2.45 mM ABTS and reacted in the dark at 23° C. for 16 hours. The concentration of the ABTS solution was measured at an absorbance of 734 nm, and was adjusted using distilled water so as to have an absorbance of 0.70*?*. 0.1 ml of the sample and 3.9 ml of ABTS solution were mixed, vortexed, and reacted at room temperature for 6 minutes. After the reaction was completed, absorbance was measured at 734 nm with a UV/Vis-spectrophotometer (U-1800, Hitachi). ABTS radical scavenging activity was expressed as a reduction ratio (%) of absorbance based on the control.
다. Reducing power All. reducing power
환원력은 (Yildirim, A., Mavi, A. and Kara, A. A. 2001)의 방법을 변형시켜 측정하였다. 각 시료 1 ml에 2.5 ml의 phosphate buffer (0.2 M, pH 6.6)와 2.5 ml의 potassium ferricyanide (1%, w/v)를 가하여 교반한 후, 50℃에서 30분간 반응시켰다. 2.5 ml의 trichloroacetic acid (10%, w/v)를 반응액에 첨가하여 반응을 종료해준 뒤 3,000 rpm으로 10분간 원심분리 하였다. 상층액의 1 ml을 취해 시험관에 담고 1 ml의 증류수와 0.2 ml의 FeCl3 (0.1%, w/v)을 첨가하여 UV/Vis-spectrophotometer (Hitachi)로 700 nm에서 흡광도를 측정하였다. The reducing power was measured by modifying the method of (Yildirim, A., Mavi, A. and Kara, A. A. 2001). To 1 ml of each sample, 2.5 ml of phosphate buffer (0.2 M, pH 6.6) and 2.5 ml of potassium ferricyanide (1%, w/v) were added and stirred, followed by reaction at 50° C. for 30 minutes. After the reaction was terminated by adding 2.5 ml of trichloroacetic acid (10%, w/v) to the reaction solution, it was centrifuged at 3,000 rpm for 10 minutes. Take 1 ml of the supernatant, put it in a test tube, add 1 ml of distilled water and 0.2 ml of FeCl3 (0.1%, w/v), and measure the absorbance at 700 nm with a UV/Vis-spectrophotometer (Hitachi).
라. Hydrogen peroxide scavenging activity la. Hydrogen peroxide scavenging activity
과산화수소(H2O2)의 소거활성능은 (M, H. E. 1985)의 방법을 이용하여 시료들을 100 μl씩 96 microwell plate에 넣고 20 μl의 hydrogen peroxide (Junsei Chemical Co., Ltd, Japan)를 첨가시켜 37℃의 incubator에서 5분 동안 반응시켰다. 반응이 끝난 후, 1.25 mM ABTS (Sigma-Aldrich, Co.)와 peroxidase (1 unit/ml; Sigma-Aldrich, Co., ST. Louis, MO, USA)를 각각 30 μl씩 첨가하고 교반하여 37℃의 incubator에서 10분간 반응시킨 후, 405 nm에서 microplate reader를 이용하여 흡광도를 측정하였다.The scavenging activity of hydrogen peroxide (H2O2) is (M , HE 1985), 100 μl of each sample was placed in a 96 microwell plate, 20 μl of hydrogen peroxide (Junsei Chemical Co., Ltd, Japan) was added, and reacted in an incubator at 37° C. for 5 minutes. After the reaction was completed, 30 μl of each of 1.25 mM ABTS (Sigma-Aldrich, Co.) and peroxidase (1 unit/ml; Sigma-Aldrich, Co., ST. Louis, MO, USA) was added and stirred at 37°C. After incubation for 10 minutes in the incubator, absorbance was measured at 405 nm using a microplate reader.
마. SOD-like activity mind. SOD-like activity
SOD 유사 활성능은 (Marklund S, Marklund G. 1975)의 방법으로 측정되었다. 즉, 각 시료 10 μl에 Tris-HCl buffer (pH 8.2) 150 μl와 7.2 mM pyrogallol (Sigma-Aldrich) 10 μl를 넣고 혼합하여 실온에서 10분간 반응시켰다. 그 후 1N HCl 50 μl를 첨가하여 반응을 정지시키고, ELISA reader (Thermo Scientific)를 사용하여 420nm에서 흡광도를 측정하였다. SOD-like activity was measured by the method (Marklund S, Marklund G. 1975). That is, 150 μl of Tris-HCl buffer (pH 8.2) and 10 μl of 7.2 mM pyrrogallol (Sigma-Aldrich) were added to 10 μl of each sample, mixed, and reacted at room temperature for 10 minutes. After that, 50 μl of 1N HCl was added to stop the reaction, and absorbance was measured at 420 nm using an ELISA reader (Thermo Scientific).
(콩나물·헛개 혼합물의 항산화 활성 검증) (Verification of antioxidant activity of bean sprouts/Hunggae mixture)
2) 결과 및 고찰 2) Results and considerations
가. DPPH radical 소거활성 go. DPPH radical scavenging activity
양성 대조구인 0.1% α-tocopherol과 0.1% BHT는 60.93% 및 66.30%의 DPPH 라디칼 소거 활성을 보였다. 그러나 BH는 89.94%로 양성 대조군 보다 높게 측정되었으며, 각각 83.94% 및 83.26%의 활성을 가지는 BSM 및 HDE와 유의적인 차이를 나타내었다. As positive controls, 0.1% α-tocopherol and 0.1% BHT showed DPPH radical scavenging activity of 60.93% and 66.30%. However, BH was measured to be 89.94% higher than that of the positive control group, and showed a significant difference from BSM and HDE having activities of 83.94% and 83.26%, respectively.
15. DPPH radical 소거 활성 변화. 15. Changes in DPPH radical scavenging activity.
나. 만성 알코올 투여 동물에서의 간 조직 변화 관찰 me. Observation of liver tissue changes in chronic alcohol-administered animals
만성적 알코올을 섭취한 쥐의 간을 적출하여 형태학적 및 병리학적 관찰을 한 결과는 다음과 같다. 형태학적 관찰에서 N군에 비해 알코올만 단독 투여한 C군의 간이 황갈색을 띠는 것을 확인 할 수 있었고, 시료들을 투여한 군에서 C군 보다 개선되어 적갈색을 띤다고 판단 할 수 있었다. H&E 염색법을 이용하여 간 조직에서 지방의 변화를 관찰한 결과는 C군에서 간 세포 내 지질이 증가하였으며, 각 시료들을 처리하였을 때 지질이 감소하는 것을 확인 할 수 있었다. 특히, BH군에서 N군과 비슷한 경향을 나타냈다. The results of morphological and pathological observations of livers from rats chronically ingested with alcohol are as follows. From the morphological observation, it was confirmed that the liver of group C treated with alcohol alone was yellowish-brown compared to group N. As a result of observing the change of fat in the liver tissue using the H&E staining method, it was confirmed that the intracellular lipids in the liver cells increased in group C, and the lipids decreased when each sample was treated. In particular, the BH group showed a similar trend to the N group.
Fig. 만성 알코올 투여 쥐 간 조직의 형태학적 관찰. N: Normal, C: Alcohol, PC: Silymarin, BSM: Bean sprout extract, HDE: 1.5% Hovenia dulcis extract, BH: Bean sprout·Hovenia dulcis extract.Fig. Morphological observation of liver tissue from chronic alcohol-administered rats. N: Normal, C: Alcohol, PC: Silymarin, BSM: Bean sprout extract, HDE: 1.5% Hovenia dulcis extract, BH: Bean sprout Hovenia dulcis extract.
Fig. 만성 알코올 투여 쥐 간 조직의 H&E 염색을 통한 병리학적 관찰. N: Normal, C: Alcohol, PC: Silymarin, BSM: Bean sprout extract, HDE: 1.5% Hovenia dulcis extract, BH: Bean sprout·Hovenia dulcis extract.Fig. Pathological observation through H&E staining of chronic alcohol-administered rat liver tissue. N: Normal, C: Alcohol, PC: Silymarin, BSM: Bean sprout extract, HDE: 1.5% Hovenia dulcis extract, BH: Bean sprout Hovenia dulcis extract.
Claims (8)
상기 육수는 물 100중량부에 건다시마와 건표고버섯을 포함하는 부재료혼합물을 70~80중량부를 혼합하고, 상기 90~105℃의 온도에서 추출하여 부재료조성물을 제조하는 단계;
상기 부재료조성물에 콩나물 35~40중량부를 혼합한 다음 100~110℃의 온도로 가열하여 콩나물추출액을 제조하는 단계; 및
상기 콩나물추출액 100중량부에 헛개나무농축액 1~2중량부를 혼합하여 콩나물 육수를 제조하는 것을 특징으로 하는 콩나물 추출물을 함유한 숙취해소와 간기능 개선 효능을 갖는 육수조성물의 제조방법
In the method for producing broth containing bean sprout extract, which has the effect of relieving hangover and improving liver function,
The broth is prepared by mixing 70 to 80 parts by weight of a sub-ingredient mixture containing dried kelp and dried shiitake mushrooms in 100 parts by weight of water, and extracting it at a temperature of 90 to 105° C. to prepare a sub-ingredient composition;
Preparing a bean sprouts extract by mixing 35-40 parts by weight of bean sprouts with the auxiliary material composition and then heating to a temperature of 100-110°C; and
Method for producing a broth composition having the effect of relieving a hangover and improving liver function containing bean sprouts extract, characterized in that the bean sprouts broth is prepared by mixing 1 to 2 parts by weight of the bean sprouts extract with 100 parts by weight of the bean sprouts extract
상기 부재료혼합물에 포함되는 건다시마는 0.1~1중량부, 건표고버섯은 0.1~5중량부를 혼합하여 끓는 온도에서 1~2분 동안 가열하여 추출하는 것을 특징으로 하는 콩나물 추출물을 함유한 숙취해소와 간기능 개선 효능을 갖는 육수조성물의 제조방법
The method of claim 1,
A hangover relieving agent containing bean sprouts extract, characterized in that 0.1 to 1 parts by weight of dried kelp contained in the auxiliary material mixture and 0.1 to 5 parts by weight of dried shiitake mushrooms are mixed and extracted by heating at a boiling temperature for 1 to 2 minutes Method for producing broth composition having liver function improvement effect
상기 부재료혼합물에 바지락살 30~40중량부, 키조개관자 5~10중량부, 홍합살 5~10중량부를 더 포함하여 혼합한 다음 100~110℃의 온도에서 100~200분 동안 가열하여 추출하는 것을 특징으로 하는 콩나물 추출물을 함유한 숙취해소와 간기능 개선 효능을 갖는 육수조성물의 제조방법
3. The method of claim 1 or 2,
30-40 parts by weight of clam meat, 5-10 parts by weight of clams, and 5-10 parts by weight of mussels in the auxiliary material mixture are further mixed and then heated at a temperature of 100-110° C. for 100-200 minutes to extract Method for producing a broth composition containing bean sprouts extract characterized in that it has the effect of relieving hangover and improving liver function
상기 부재료혼합물에 고추씨 1~5중량부를 혼합한 다음 90내지 100℃의 온도에서 10~30분 동안 가열하는 것을 특징으로 하는 콩나물 추출물을 함유한 숙취해소와 간기능 개선 효능을 갖는 육수조성물의 제조방법
3. The method of claim 1 or 2,
1 to 5 parts by weight of red pepper seeds are mixed with the sub-ingredient mixture, and then heated for 10 to 30 minutes at a temperature of 90 to 100 ° C. Method for producing a broth composition having the effect of relieving a hangover and improving liver function containing bean sprouts extract
상기 부재료혼합물에 고추씨 1~5중량부를 혼합한 다음 90내지 100℃의 온도에서 10~30분 동안 가열하는 것을 특징으로 하는 콩나물 추출물을 함유한 숙취해소와 간기능 개선 효능을 갖는 육수조성물의 제조방법
4. The method of claim 3,
1 to 5 parts by weight of red pepper seeds are mixed with the sub-ingredient mixture, and then heated for 10 to 30 minutes at a temperature of 90 to 100 ° C. Method for producing a broth composition having the effect of relieving a hangover and improving liver function containing bean sprouts extract
상기 부재료혼합물에 조미원료를 더 포함하되, 상기 조미원료는 대파 3~10중량부, 무 2~8중량부, 양파 2~6중량부, 소금 0.5 내지 2중량부를 혼합한 다음 98~120℃의 온도에서 20~40분 동안 가열하는 것을 특징으로 하는 콩나물 추출물을 함유한 숙취해소와 간기능 개선 효능을 갖는 육수조성물의 제조방법
The method of claim 1 or 2, wherein
A seasoning raw material is further included in the auxiliary material mixture, wherein the seasoning raw material is mixed with 3 to 10 parts by weight of green onion, 2 to 8 parts by weight of radish, 2 to 6 parts by weight of onion, and 0.5 to 2 parts by weight of salt, followed by a temperature of 98 to 120 ° C. Method for producing a broth composition having the effect of relieving a hangover and improving liver function containing bean sprout extract, characterized in that it is heated at a temperature for 20 to 40 minutes
상기 부재료혼합물에 조미원료를 더 포함하되, 상기 조미원료는 대파 3~10중량부, 무 2~8중량부, 양파 2~6중량부, 소금 0.5 내지 2중량부를 혼합한 다음 98~102℃의 온도에서 20~40분 동안 가열하는 것을 특징으로 하는 콩나물 추출물을 함유한 숙취해소와 간기능 개선 효능을 갖는 육수조성물의 제조방법
4. The method of claim 3,
A seasoning raw material is further included in the sub-ingredient mixture, wherein the seasoning raw material is mixed with 3 to 10 parts by weight of green onion, 2 to 8 parts by weight of radish, 2 to 6 parts by weight of onion, and 0.5 to 2 parts by weight of salt, followed by a temperature of 98 to 102 ° C. Method for producing a broth composition having the effect of relieving a hangover and improving liver function containing bean sprout extract, characterized in that it is heated at a temperature for 20 to 40 minutes
상기 부재료혼합물에 조미원료를 더 포함하되, 상기 조미원료는 대파 3~10중량부, 무 2~8중량부, 양파 2~6중량부, 소금 0.5 내지 2중량부를 혼합한 다음 98~102℃의 온도에서 20~40분 동안 가열하는 것을 특징으로 하는 콩나물 추출물을 함유한 숙취해소와 간기능 개선 효능을 갖는 육수조성물의 제조방법
6. The method of claim 5,
A seasoning raw material is further included in the sub-ingredient mixture, wherein the seasoning raw material is mixed with 3 to 10 parts by weight of green onion, 2 to 8 parts by weight of radish, 2 to 6 parts by weight of onion, and 0.5 to 2 parts by weight of salt, followed by a temperature of 98 to 102 ° C. Method for producing a broth composition having the effect of relieving a hangover and improving liver function containing bean sprout extract, characterized in that it is heated at a temperature for 20 to 40 minutes
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