KR20220083879A - Composition for promoting hair growth - Google Patents

Abstract

The present disclosure relates to a composition for promoting hair growth comprising ascorbic acid glycosides and caffeine. The composition for promoting hair growth may promote hair growth by inhibiting apoptosis of myoblast cells.

Description

Composition for promoting hair growth {COMPOSITION FOR PROMOTING HAIR GROWTH}

The present specification relates to a composition for promoting hair growth.

Hair is primarily responsible for the defense function to protect the scalp from external stimuli such as ultraviolet rays, and is responsible for the aesthetic function of expressing external images. However, in modern society, hair loss occurs frequently due to natural factors such as environmental pollution, strong ultraviolet rays, and physiological factors such as stress and hormonal imbalance. Accordingly, there is an urgent need for materials for its prevention and treatment.

The hair follicle, a physiological organ that produces hair, is maintained during the development process after birth, during the anagen phase, in which the generated hair actively grows, in the catagen phase, in which the hair is degenerated, and until hair loss. It is involved in hair growth, maintenance, and loss by repeating the hair cycle divided into a telogen phase and an exogen phase in which hair loss occurs. According to many studies, the activity of this hair follicle is caused by outer root sheath cells, and in particular, proliferation and differentiation of outer root sheath cells are primarily involved in the progression of the hair growth cycle and hair formation. During the growth phase of hair growth, proliferation and differentiation of the outer myoblast cells occur actively so that the hair can grow, but when cell division of the outer myoblast cells is inhibited or apoptosis occurs, hair growth is restricted.

Therefore, proliferation and death of external myoblast cells are closely related to hair growth, so by inducing their proliferation, it is possible to lengthen the growth phase or inhibit apoptosis of external myoblast cells to promote hair growth and improve hair loss.

Minoxidil and finasteride, which are conventionally used as agents for promoting hair growth and hair loss, have side effects such as systemic hair growth and decreased sexual function, and the specific mechanism of action of minoxidil has not been clearly elucidated. Therefore, there is a need to develop a biological material that promotes proliferation or suppresses apoptosis and has less side effects.

Republic of Korea Patent Publication No. 10-2019-0091162

In one aspect, the present disclosure is to provide a composition for promoting hair growth.

In one aspect, the present invention provides a composition for promoting hair growth comprising ascorbic acid glycosides and caffeine.

In an exemplary embodiment, the ascorbic acid glycoside may be ascorbic acid 2-glucoside.

In an exemplary embodiment, the weight ratio of the ascorbic acid glycoside and caffeine may be 1: 0.5 to 1.5.

In an exemplary embodiment, the ascorbic acid glycoside may be administered at a dose of 0.5 to 15 mg/kg/day, and the caffeine may be administered at a dose of 0.5 to 15 mg/kg/day.

In an exemplary embodiment, the composition may inhibit apoptosis of myoblast cells.

In an exemplary embodiment, the composition may be a composition for external application to the skin.

In an exemplary embodiment, the composition may be a food, cosmetic or pharmaceutical composition.

In one aspect, the present invention provides a composition comprising an ascorbic acid glycoside; compositions comprising caffeine; And it provides a kit for promoting hair growth comprising instructions.

In an exemplary embodiment, the instructions describe that the composition comprising the ascorbic acid glycoside and the composition comprising caffeine are administered in combination in a weight ratio of 1: 0.5 to 1.5.

In one aspect, the composition for promoting hair growth according to the present invention has excellent hair growth promoting effect due to the synergistic action of ascorbic acid glycoside and caffeine, and can prevent hair loss.

In one aspect, the composition for promoting hair growth according to the present invention can inhibit apoptosis of myoblast cells.

1 is a graph showing the apoptosis inhibitory efficacy of the hair growth promoting composition according to an embodiment of the present invention.

Hereinafter, the present invention will be described in detail.

In one aspect, the present invention provides a composition for promoting hair growth comprising ascorbic acid glycosides and caffeine.

In one embodiment, the ascorbic acid glycoside may be ascorbic acid 2-glucoside (AA2G).

In one embodiment, the weight ratio of the ascorbic acid glycoside and caffeine may be 1: 0.5 to 1.5. When the weight ratio of ascorbic acid glycoside and caffeine is out of the above range, the hair growth promoting efficacy is reduced. Specifically, the weight ratio of the ascorbic acid glycoside and caffeine may be 1: 0.5 to 1.5, preferably 1: 0.5 to 1.3, preferably 1: 0.7 to 1.3, preferably 1: 1.

In one embodiment, the ascorbic acid glycoside may be administered at a dosage of 0.5 to 15 mg/kg/day. If the dosage of the ascorbic acid glycoside is less than 0.5 mg/kg/day, it may be difficult to exhibit the effect according to the present invention, and if it is more than 15 mg/kg/day, it may cause irritation to the skin. Specifically, the ascorbic acid glycoside is 0.5 mg/kg/day or more, 0.6 mg/kg/day or more, 0.7 mg/kg/day or more, 0.8 mg/kg/day or more, 0.9 mg/kg/day or more, 1 mg /kg/day or more, 1.1 mg/kg/day or more, 1.2 mg/kg/day or more, 1.3 mg/kg/day or more, 1.4 mg/kg/day or more, 1.5 mg/kg/day or more, 1.6 mg/kg /day or more, 1.7 mg/kg/day or more, 1.8 mg/kg/day or more, 1.9 mg/kg/day or more, 2 mg/kg/day or more, 2.1 mg/kg/day or more, 2.2 mg/kg/day or more or more, 2.3 mg/kg/day or more, 2.4 mg/kg/day or more, 2.5 mg/kg/day or more, 2.6 mg/kg/day, 2.7 mg/kg/day or more, 2.8 mg/kg/day or more, 2.9 ≥ mg/kg/day, ≥ 3 mg/kg/day, or ≥ 4 mg/kg/day, but ≤ 15 mg/kg/day, ≤ 14.9 mg/kg/day, ≤ 14.8 mg/kg/day, 14.7 mg /kg/day or less, 14.6 mg/kg/day or less, 14.5 mg/kg/day or less, 14.4 mg/kg/day or less, 14.3 mg/kg/day or less, 14.2 mg/kg/day or less, 14.1 mg/kg /day or less, 14 mg/kg/day or less, 13.9 mg/kg/day or less, 13.8 mg/kg/day or less, 13.7 mg/kg/day or less, 13.6 mg/kg/day or less, 13.5 mg/kg/day or less It may be administered at a dosage of 13 mg/kg/day or less, 12.5 mg/kg/day or less, 12 mg/kg/day or less, 11.5 mg/kg/day or less, or 11 mg/kg/day or less.

In one embodiment, the caffeine may be administered in a dosage of 0.5 to 15 mg/kg/day. If the dosage of caffeine is out of the above range, it may be difficult to exhibit the effect according to the present invention. Specifically, the caffeine is 0.5 mg/kg/day or more, 0.6 mg/kg/day or more, 0.7 mg/kg/day or more, 0.8 mg/kg/day or more, 0.9 mg/kg/day or more, 1 mg/kg /day or more, 1.1 mg/kg/day or more, 1.2 mg/kg/day or more, 1.3 mg/kg/day or more, 1.4 mg/kg/day or more, 1.5 mg/kg/day or more, 1.6 mg/kg/day or more or more, 1.7 mg/kg/day or more, 1.8 mg/kg/day or more, 1.9 mg/kg/day or more, 2 mg/kg/day or more, 2.1 mg/kg/day or more, 2.2 mg/kg/day or more, 2.3 mg/kg/day or more, 2.4 mg/kg/day or more, 2.5 mg/kg/day or more, 2.6 mg/kg/day, 2.7 mg/kg/day or more, 2.8 mg/kg/day or more, 2.9 mg/ kg/day or more, 3 mg/kg/day or more, or 4 mg/kg/day, but not more than 15 mg/kg/day, less than 14.9 mg/kg/day, less than 14.8 mg/kg/day, less than 14.7 mg/kg /day or less, 14.6 mg/kg/day or less, 14.5 mg/kg/day or less, 14.4 mg/kg/day or less, 14.3 mg/kg/day or less, 14.2 mg/kg/day or less, 14.1 mg/kg/day or less or less, 14 mg/kg/day or less, 13.9 mg/kg/day or less, 13.8 mg/kg/day or less, 13.7 mg/kg/day or less, 13.6 mg/kg/day or less, 13.5 mg/kg/day or less, 13 mg/kg/day or less, 12.5 mg/kg/day or less, 12 mg/kg/day or less, 11.5 mg/kg/day or less, or 11 mg/kg/day or less.

In one embodiment, the composition may inhibit apoptosis (apoptosis) of outer root sheath cells. The outer myoblast cells are cells that make up the hair follicle, and they actively divide during the hair growth period to allow the hair to grow. The composition according to the present invention can inhibit apoptosis of external myoblast cells, thereby promoting hair growth and preventing hair loss.

In one embodiment, the route of administration of the composition is not limited, but may be preferably transdermally or externally applied to the skin.

In one embodiment, the composition may be a cosmetic composition. The cosmetic composition is, for example, softening lotion, astringent lotion, nourishing lotion, nourishing cream, massage cream, eye cream, eye essence, essence, cleansing cream, cleansing lotion, cleansing foam, cleansing water, pack, powder, body lotion , body cream, body essence, body cleaner, hair dye, shampoo, conditioner, hair dressing, hair conditioner, ointment, gel, cream, patch, spray, powder, and skin adhesion type, but are not limited thereto.

In addition, in each formulation, other components other than the above essential components can be appropriately selected and formulated by those skilled in the art without difficulty depending on the type or purpose of use of other external preparations.

The cosmetic composition may be provided in any formulation suitable for topical application. For example, it may be provided in the form of a solution, an emulsion obtained by dispersing an oil phase in an aqueous phase, an emulsion obtained by dispersing an aqueous phase in an oil phase, a suspension, a solid, a gel, a powder, a paste, a microneedle, a foam, or an aerosol composition. have. Compositions of such formulations can be prepared according to conventional methods in the art.

The cosmetic composition according to the present specification may further include functional additives and components included in general cosmetic compositions in addition to the compounds of the present specification. The functional additive may include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids, and seaweed extract. The cosmetic composition according to the present specification may include other ingredients that can give a synergistic effect to the main effect, preferably within a range that does not impair the main effect. In addition, the cosmetic composition according to the present specification may further include a moisturizing agent, an emollient, a surfactant, a UV absorber, a preservative, a bactericide, an antioxidant, a pH adjuster, an organic and inorganic pigment, a fragrance, a cooling agent or a limiting agent. The blending amount of the component can be easily selected by those skilled in the art within the range that does not impair the purpose and effect of the present specification, and the blending amount may be 0.001 to 10% by weight, specifically 0.01 to 3% by weight, based on the total weight of the composition. have.

In one embodiment, the composition may be a food composition. The formulation of the food composition is not particularly limited, but, for example, may be formulated as tablets, granules, pills, powders, liquids such as drinks, caramel, gels, bars, tea bags, and the like. In addition to the active ingredient, the food composition of each dosage form can be appropriately selected and formulated by those skilled in the art without difficulty depending on the dosage form or purpose of use in addition to the active ingredient, and a synergistic effect may occur when applied simultaneously with other raw materials.

The food composition according to one embodiment includes various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavoring agents, coloring agents and enhancers (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the food compositions according to an embodiment may include natural fruit juice and pulp for the production of fruit juice beverages and vegetable beverages. These components may be used independently or in combination. The proportion of these additives is not so important, but is generally included in the range of 0 to about 50 parts by weight per 100 parts by weight of the composition according to an embodiment.

In one embodiment, the composition may be a pharmaceutical composition. The pharmaceutical composition may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously, or the like. Formulations for oral administration may be tablets, pills, soft and hard capsules, granules, powders, fine granules, liquids, emulsions, or pellets, but are limited thereto it is not Formulations for parenteral administration may be solutions, suspensions, emulsions, gels, injections, drops, suppositories, patches, or sprays, but is not limited thereto. The formulations can be easily prepared according to conventional methods in the art, and surfactants, excipients, wettable powders, emulsification accelerators, suspending agents, salts or buffers for osmotic pressure control, colorants, spices, stabilizers, preservatives, preservatives or Other commercially available adjuvants may be further included.

In one aspect, the present invention provides a composition comprising an ascorbic acid glycoside; compositions comprising caffeine; And it provides a kit for promoting hair growth comprising instructions.

In one embodiment, the instructions describe that the composition containing the ascorbic acid glycoside and the composition containing caffeine are administered in combination in a weight ratio of 1: 0.5 to 1.5. Specifically, in the description, the composition containing the ascorbic acid glycoside and the composition containing caffeine are 1: 0.5 to 1.5, preferably 1: 0.5 to 1.3, preferably 1: 0.7 to 1.3, preferably 1: 1 Concomitant administration in a weight ratio of

Hereinafter, the present invention will be described in more detail through examples and the like. These examples are only for illustrating the present invention, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not to be construed as being limited by these examples.

[Experimental Example 1] Efficacy of inhibiting apoptosis of myoblast cells

Outer root sheath cells (Kyungpook National University) were seeded at 20000 cells/well in a 96-well plate coated with collagen I, and then cultured at 37° C. and 5% CO ₂ conditions for 24 hours. After washing twice with DPBS, the samples (caffeine 1ppm, caffeine 10ppm, AA2G (ascorbic acid 2-glucoside) 1ppm, AA2G 10ppm, caffeine 1ppm and AA2G 1ppm, caffeine 10ppm and AA2G 10ppm together) were each treated for 4 hours. processed. After removing the samples, apoptosis inducing factor, H ₂ O ₂ , was treated together with the samples for 24 hours. After washing once with DPBS, cell viability was measured by CCK8 assay, and the results are shown in FIG. 1 .

As can be seen in FIG. 1 , when caffeine or AA2G was treated alone, apoptosis of outer myoblast cells by H ₂ O ₂ was not inhibited, but when caffeine and AA2G were used in combination, apoptosis of outer myoblast cells was inhibited. was able to confirm that

[Formulation Example 1] Soap

Soap was prepared in a conventional manner according to the composition shown in Table 1 below.

ingredient content Ascorbic acid 2-glucoside 1 g Caffeine 1 g titanium dioxide 0.5 g polyethylene glycol 1 g glycerin 0.5 g Ethylenediaminetetraacetic acid 0.5 g salt 1 g pigment 0.2 g soapy scent 0.2 g soap base 94.1 g

[Formulation Example 2] Liquid ampoule

A liquid ampoule was prepared in a conventional manner according to the composition shown in Table 2 below.

ingredient content Ascorbic acid 2-glucoside 10 mg Caffeine 10 mg Indigestible maltodextrin 100 mg citric acid 10 mg Spices 5 mg Purified water 28.5 g

[Formulation Example 3] Cream

Creams were prepared in a conventional manner according to the composition shown in Table 3 below.

ingredient content Ascorbic acid 2-glucoside 1.5 g Caffeine 1.5 g Polyethylene glycol monostearate 4 g Self-emulsifying glycerin monostearate 10 g cetyl alcohol 8 g squalene 10 g tri2-glyceryl ethyl henate 10 g sphingoglycolipids 2 g 1,3-butylene glycol 14 g Purified water 189 g

[Formulation Example 4] Cosmetic liquid formulation

According to the composition shown in Table 4 below, a cosmetic liquid formulation was prepared in a conventional manner.

ingredient content Ascorbic acid 2-glucoside 1.5 g Caffeine 1.5 g Hydroxyethylene Cellulose 15 g xanthan gum 4 g 1,3-butylene glycol 10 g glycerin 8 g Sodium Hyaluronate 5 g Purified water 155 g

[Formulation Example 5] Soft capsules

Ascorbic acid 2-glucoside 60 mg, caffeine 60 mg, L-carnitine 160 mg, soybean oil 320 mg, palm oil 2 mg, hydrogenated vegetable oil 8 mg, yellow wax 4 mg and lecithin 6 mg were mixed, and filled into 1 capsule according to a conventional method to prepare a soft capsule. did

[Formulation Example 6] Tablet

60 mg of ascorbic acid 2-glucoside, 60 mg of caffeine, 500 mg of galactooligosaccharide, 80 mg of lactose and 220 mg of maltose were mixed and granulated using a fluidized bed dryer. prepared.

[Formulation Example 7] Granules

50 mg of ascorbic acid 2-glucoside, 50 mg of caffeine, 250 mg of anhydrous crystalline glucose, and 550 mg of starch were mixed, formed into granules using a fluidized bed granulator, and then filled in a bag to prepare granules.

[Formulation Example 8] Drink formulation

After mixing 50 mg of ascorbic acid 2-glucoside, 50 mg of caffeine, 10 g of glucose, 0.6 g of citric acid, and 25 g of liquid oligosaccharide, 500 ml of purified water is added, and 200 ml of each bottle is filled. After filling the bottle, it was sterilized at 130° C. for 4 to 5 seconds to prepare a drink beverage.

Claims (10)

A composition for promoting hair growth comprising ascorbic acid glycosides and caffeine. According to claim 1,
wherein the ascorbic acid glycoside is ascorbic acid 2-glucoside. According to claim 1,
The weight ratio of the ascorbic acid glycoside and caffeine is 1: 0.5 to 1.5, the composition. According to claim 1,
The ascorbic acid glycoside is administered at a dosage of 0.5 to 15 mg/kg/day,
The caffeine is administered in a dosage of 0.5 to 15 mg/kg/day, the composition. According to claim 1,
The composition inhibits apoptosis of outer root sheath cells. 6. The method according to any one of claims 1 to 5,
The composition is a composition for external application to the skin, the composition. 6. The method according to any one of claims 1 to 5,
The composition is a food composition. 6. The method according to any one of claims 1 to 5,
The composition is a cosmetic composition, composition. 6. The method according to any one of claims 1 to 5,
The composition is a pharmaceutical composition. As a kit for promoting hair growth,
The kit comprises a composition comprising ascorbic acid glycoside; compositions comprising caffeine; and instructions;
In the instructions, it is described that the composition containing the ascorbic acid glycoside and the composition containing caffeine are administered in combination in a weight ratio of 1: 0.5 to 1.5, a kit for promoting hair growth.

Images