KR20220080366A - Preparing method of Tenebrio molitor extract for cosmetics - Google Patents

Abstract

The present invention relates to a functional cosmetic composition comprising, as an active ingredient, an extract of Mealworm from Brown Mealtimes, which has excellent effects such as recovery, moisturizing, and cleaning of damaged hair and skin, and a method for manufacturing the same, comprising: The cosmetic composition contains a wealth of useful peptide components, so it has excellent soothing and damage recovery effects on the scalp or skin cells, and can be safely used compared to conventional cosmetics or hair loss treatment ingredients because it uses natural materials.

Description

Manufacturing method of brown mealworm extract for cosmetics {Preparing method of Tenebrio molitor extract for cosmetics}

The present invention relates to a method for producing a Melancholic Mealworm Extract for cosmetics, and more particularly, to a functional cosmetic composition comprising, as an active ingredient, an Mealworm Meal brown extract having excellent effects such as recovery, moisturizing, and cleaning of damaged hair and skin, and a method for producing the same is about

The brown mealworm ( Tenebrio molitor ) is an insect belonging to the family Melancholy and is distributed all over the world including Korea. Brown mealworm larva, also called mealworm, has a relatively short metamorphosis period, feeds on grains, and is clean, so it is mainly bred as food for companion animals, edible insects, or pets.

Mealworm brown is known to exhibit useful effects on the human body, such as antioxidant, skin whitening, improvement of inflammatory diseases, and promotion of hair growth, and several prior arts related to functional foods, cosmetics, or pharmaceutical compositions containing Mealworm brown extract have been disclosed.

In order to industrially use brown mealworm exhibiting various functionalities, a brown mealworm extract was conventionally prepared using a hot water extraction method using high-temperature water. Because the various functionalities of Mealworm Mealworm Extract can show a significant difference in the expression level depending on the extraction method, in order to actually use Mealworm Mealworm extract in a product, it is necessary for the product to It is necessary to optimize the extraction method to show the effect significantly.

Accordingly, the present inventors have invented a method for preparing a brown mealworm extract that can minimize protein denaturation in order to improve the functionality of the brown mealworm extract used in cosmetics such as soap, hair shampoo or lotion, such as damaged skin cell recovery, in particular. .

Republic of Korea Patent Publication No. 10-2018-006699 Republic of Korea Patent Publication No. 10-2019-0013416

An object of the present invention is to provide a method for preparing an extract of Mealworm Brown using a low-temperature enzyme treatment method capable of minimizing denaturation of Mealworm protein.

Another object of the present invention is to provide a cosmetic composition containing the brown mealworm extract.

In order to achieve the above object, the present invention comprises the steps of crushing the brown mealworm larvae; Treating the pulverized mealworm larvae with a proteolytic enzyme to cause a proteolytic reaction; pasteurizing the reactant; and cooling the reactant and then filtering it.

The protein hydrolysis step is a process for increasing the content of peptides related to skin regeneration among the functional substances contained in the brown mealworm larva to use the brown mealworm extract as a functional cosmetic material, and depending on the type of proteolytic enzyme and treatment conditions Since the type and molecular weight of the produced peptide may vary, there may be a significant difference in the skin regenerating effect of the brown mealworm extract.

Examples of proteolytic enzymes used to prepare protein hydrolysates of edible insects include Flavozyme, Neutrase, Alcalase, Papain, Bromelain. , Pescalase, Protamax, Protease A, etc., but in terms of skin regeneration effect, the proteolytic enzyme used in the production method of the brown mealworm extract of the present invention is Alcalase ( Alcalase) is preferred.

In one embodiment of the present invention, the treatment condition of the alcalase is a mixture of 50% by weight of ground mealworm larvae pulverized to a size of 1 to 10 mm, 49% by weight of purified water and 1% by weight of alcalase, It is preferable to mix and stir at 43-47° C. for 10 to 14 hours, and most preferably, react at 45° C. for 12 hours.

The step of pasteurizing the hydrolysis reaction product in the method for preparing the brown mealworm extract of the present invention is to stop the enzymatic reaction under conditions in which the peptide produced through the hydrolysis reaction is not denatured in order to obtain an extract having a desired peptide content (activation). ), in one embodiment of the present invention, the pasteurization is preferably made at 60 ~ 65 ℃ for 30 minutes or more, and most preferably at 63 ℃ for 30 ~ 60 minutes.

The method for producing a brown mealworm extract of the present invention includes a step of cooling the hydrolyzate after the pasteurization (enzyme inactivation) step to prevent protein denaturation, and removing impurities through filtration. In one embodiment of the present invention, the cooling is preferably cooled to 10 ℃ or less. In one embodiment of the present invention, it is preferable to use a 300 mesh filtration filter in the filtration process.

In one embodiment of the present invention, the method for preparing the Melancholy Mealtimes extract of the present invention comprises the steps of pulverizing Mealworm larvae of Brown Meal to a size of 1 to 10 mm; causing a protein hydrolysis reaction while mixing and stirring 50% by weight of the brown mealworm larvae pulverized product, 49% by weight of purified water, and 1% by weight of alcalase at 43 to 47° C. for 10 to 14 hours; pasteurizing the reactant at 60 to 65° C. for 30 to 60 minutes; and cooling the reactant to 10° C. or less, followed by filtering.

In another aspect, the present invention provides a cosmetic composition containing the Mealworm brown extract prepared by the above manufacturing method.

The cosmetic composition includes, for example, lotion, emulsion, skin, toner, emulsion, lotion, essence, cream, makeup base, foundation, powder, pack, gel, shampoo, conditioner, makeup remover, detergent, soap, cleansing foam, cleansing lotion, Cleansing cream, body lotion, body cleanser, hair tonic, hair conditioner, hair essence, hair lotion, hair treatment, hair cream, hair oil, hair gel, etc., including any conventionally manufactured in the art to which the present invention belongs Formulations can also be prepared.

In addition, the cosmetic composition may contain, in addition to the brown mealworm extract of the present invention, various additives necessary for the formulation of the formulation or generally used in cosmetic compositions.

For example, when the formulation is a solution or emulsion, water, ethanol, benzyl alcohol, benzyl benzoate, isopropanol, ethyl carbonate, ethyl acetate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol Solvents, solubilizers, or emulsifiers such as solvents, solubilizers, or emulsifiers may be used as carrier components, and when the formulation is a suspension, liquid diluents such as water, ethanol, and propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, etc. A suspending agent may be used, and in the case of the surfactant-containing cleansing formulation, fatty alcohol sulfate, fatty alcohol ether sulfate, sulfosuccinic acid monoester, fatty acid amide ether sulfate, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, etc. may be used. can be used In addition, when the formulation is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, etc. may be used as a carrier component, and the formulation is In the case of powder or spray, silica, aluminum hydroxide, calcium silicate, lactose, talc, polyamide powder, etc. may be used.

In one embodiment of the present invention, the cosmetic composition is soap, hair shampoo or lotion. According to the manufacturing method of general soap, hair shampoo, or lotion, when the content of the brown mealworm extract is less than 2% by weight, a significant level of skin regeneration effect does not appear, and at 6% by weight or more, a similar level of skin regeneration effect is obtained. Therefore, it is preferable that the content of the brown mealworm extract contained in the cosmetic composition of the present invention is 2 to 6% by weight.

The cosmetic composition containing the brown mealworm extract of the present invention as an active ingredient contains an abundance of useful peptide components, so it has excellent calming and damage recovery effects on the scalp or skin cells. It can be used safely compared to ingredients.

Hereinafter, the present invention will be described in detail by way of Examples. However, the following examples only illustrate the present invention, and the content of the present invention is not limited to the following examples.

[Example 1] Preparation of brown mealworm larvae extract

The present inventors measure the total protein content and sulfur-containing amino acid content, which is an indicator of the degree of hydrolysis, contained in various brown mealworm extracts prepared by different types of proteolytic enzymes and reaction conditions, and the production process of an extract showing the best effect was established, and the brown mealworm larva extract of the present invention can be prepared according to the following process.

a) After washing the brown mealworm larvae raised to a size of 4-8 cm, they were crushed and pulverized to a size of 1-10 mm by using a physical machine and instrument so that the proteolytic enzyme can work efficiently.

b) 50% (w/w) of the pulverized product, 49% (w/w) of purified water, and 1% (w/w) of Alcalase (purchased from NOVO), a proteolytic enzyme, were mixed and stirred at 45° C. (±2°C) was subjected to an enzymatic reaction for 12 hours. According to the above enzymatic reaction, the components contained in Mealworm larvae can be decomposed into small molecules to obtain an extract containing a large amount of useful peptides.

c) Brown mealworm extract by sterilizing at 63°C for 30 minutes at low temperature to minimize denaturation of the protein component contained in the extract (hydrolyzate), cooling it to 10°C or less, and then ultra-filtration using a 300 mesh filtration filter was prepared.

[Example 2] Preparation of a cosmetic composition containing a brown mealworm larva extract

Soap (Preparation Example 1) and lotion (Preparation Example 2) were prepared using the brown mealworm larva extract prepared according to Example 1, and for comparison of their effects with soap (Comparative Example 1) and A lotion (Comparative Example 2) was prepared by a general manufacturing method.

[Preparation Example 1] Preparation of Soap Containing Mealworm Extract of the Present Invention

Put vegetable fatty acid, vegetable oil, glycerin, and purified water to dissolve the oil well at 65°C to 75°C, add potassium hydroxide to complete the saponification process, which is a saponification reaction, and adjust the pH with citric acid, an acid, and adjust the pH of the present invention. Soap was prepared by adding the extract and fragrance. In order to determine the optimal content of the brown mealworm extract, soap was prepared by varying the mixing ratio as shown in Table 1, and 300 kg for each preparation example was prepared and the effects were compared.

[Comparative Example 1]

A general soap was prepared in the same manner as in Preparation Example 1 by adding chitosan, which is known to have excellent moisturizing and cleaning effects, without adding the brown mealworm extract of the present invention, and the mixing ratio is shown in Table 2 below.

[Preparation Example 2] Preparation of lotion containing brown mealworm extract of the present invention

A liquid lotion was prepared using the extract and aroma oil of Example 1. Put vegetable fatty acid, vegetable oil, glycerin, and purified water to dissolve the oil well at 65 ℃ to 75 ℃, add an emulsifier to finish the emulsification process, adjust the pH with citric acid, an acid, and then add the extract and flavor of the present invention. A lotion for basic cosmetics was prepared, and as shown in Table 3 below, the mixing ratio was varied in 5 steps to prepare a total of 500 kg by 100 kg at each step.

[Comparative Example 2]

A general lotion was prepared in the same manner as in Preparation Example 2 by adding chitosan, which is known to have excellent moisturizing and cleaning effects, without adding the brown mealworm extract of the present invention, and the mixing ratio is shown in Table 4 below.

[Example 3] Effect of cosmetic composition containing brown mealworm extract

In order to confirm the effect of the soap and lotion containing the brown mealworm extract of the present invention prepared according to Example 2, the skin condition was tested after use in the following manner.

For 50 adult males and females between the ages of 18 and 25 who have skin troubles or acne, use the products prepared according to the Preparation Examples and Comparative Examples of Example 2 for 60 days, ① overall skin Condition (0~13 points), ② degree of recovery from bacterial skin damage (0~4 points), ③ remission (0~3 points) were evaluated by scoring the degree of recovery of the skin condition, and then the average value was obtained. , the results are shown in Tables 5 to 8 below.

As can be seen from the above results, the soap and lotion containing the extract of the present invention showed a superior skin regeneration effect compared to general soaps and lotions containing chitosan in general, and contained in a ratio of 2 to 6% by weight. It can be seen that it is the most effective and economically desirable when

The embodiments of the present invention described above are exemplary, and those of ordinary skill in the art to which the present invention pertains will understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be able Therefore, the scope of the present invention is not limited to the above-described embodiments, and should be defined by all changes or modifications derived from the contents described in the claims and their equivalent concepts.

Claims (3)

crushing the brown mealworm larvae;
causing a protein hydrolysis reaction while mixing and stirring 50% by weight of the brown mealworm larvae pulverized product, 49% by weight of purified water, and 1% by weight of alcalase at 43 to 47° C. for 10 to 14 hours;
pasteurizing the reactant at 60 to 65° C. for 30 to 60 minutes; and
A method for producing a brown mealworm extract comprising the step of cooling the reactant to 10° C. or less and then filtering. A cosmetic composition containing 2 to 6% by weight of the brown mealworm extract prepared by the method of claim 1. 3. The method of claim 2,
The cosmetic composition is a composition, characterized in that soap, hair shampoo or lotion.