KR20220062435A - Comprising low molecular weight Peptide from Swimming Crab - Google Patents
Comprising low molecular weight Peptide from Swimming Crab Download PDFInfo
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- KR20220062435A KR20220062435A KR1020200147606A KR20200147606A KR20220062435A KR 20220062435 A KR20220062435 A KR 20220062435A KR 1020200147606 A KR1020200147606 A KR 1020200147606A KR 20200147606 A KR20200147606 A KR 20200147606A KR 20220062435 A KR20220062435 A KR 20220062435A
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- 235000014101 wine Nutrition 0.000 description 1
- 235000004835 α-tocopherol Nutrition 0.000 description 1
- 239000002076 α-tocopherol Substances 0.000 description 1
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Abstract
Description
본 발명은 꽃게 유래 저분자 펩타이드를 함유하는 조성물에 관한 것으로, 더욱 상세하게는 항산화 효과가 우수할 뿐만 아니라 면역증강 효과도 우수한, 꽃게 유래 저분자 펩타이드를 함유하는 조성물에 관한 것이다.The present invention relates to a composition containing a blue crab-derived low-molecular-weight peptide, and more particularly, to a composition containing a blue crab-derived low-molecular-weight peptide, which is excellent not only in the antioxidant effect but also in the immune-enhancing effect.
항산화제(antioxidant)는 산화반응을 억제하는 물질로서 활성산소 또는 산소자유라디칼이라고 하는 히드록실라디칼, 수퍼옥시드아니온, 과산화수소 등을 포함하는, 소위 산화물질의 작용을 소거 또는 감쇠시키는 물질을 총칭하는 의미이다. 저분자화합물로는 글루타티온, N-아세틸시스테인, 아스코르빈산, α-토코페롤, 부틸화히드록시아니졸(BHA), 카테킨, 케르세틴, 요산, 빌리루빈, 포도당, 플라보노이드 등이 알려져 있으며, 최근에는 레드와인 추출물, 토마토, 블루베리 등 천연 항산화 제품들이 각광을 받고 있다.Antioxidant is a substance that inhibits oxidation, and is a generic term for substances that eliminate or attenuate the action of so-called oxidizing substances, including hydroxyl radicals, superoxide anions, and hydrogen peroxide, which are called active oxygen or oxygen free radicals. it means As low molecular weight compounds, glutathione, N-acetylcysteine, ascorbic acid, α-tocopherol, butylated hydroxyanisole (BHA), catechin, quercetin, uric acid, bilirubin, glucose, flavonoids, etc. are known, and recently, red wine extract Natural antioxidant products such as , tomatoes, and blueberries are in the spotlight.
프리 라디칼에 관련된 지방의 산화, 산화적 스트레스 및 항산화제에 관한 연구들은 최근에 집중적으로 연구되어 왔다. BHA(butylated hydroxy-anisole) 및 BHT(butylated hydroxytoluene)등 합성 항산화제들이 상업화하여 사용되고 있지만 최근 이들 유도체에 의한 DNA 손상 및 독성에 대하여 밝혀지고 있다. 생리 활성 펩타이드들은 그들이 속해있던 단백질에서는 불활성화 상태로 있지만 가수분해에 의하여 생리 활성을 나타내면 호르몬과 같은 기능을 나타내는 것으로 발표되고 있다. 다양한 원료들 중에서 수산물 단백질에서 항산화, 항고혈압, 항암, 엔지오텐신 전환효소억제 기능을 나타내는 펩타이드 형태의 단백질 가수분해물들이 보고되고 있다.Studies on the oxidation of fats related to free radicals, oxidative stress and antioxidants have been intensively studied in recent years. Synthetic antioxidants such as BHA (butylated hydroxy-anisole) and BHT (butylated hydroxytoluene) have been commercialized and used, but recently, DNA damage and toxicity caused by these derivatives have been revealed. Although physiologically active peptides are in an inactivated state in the protein to which they belong, it has been reported that when they exhibit physiological activity by hydrolysis, they exhibit the same function as hormones. Among various raw materials, hydrolyzates of peptide proteins that exhibit antioxidant, antihypertensive, anticancer, and angiotensin-converting enzyme inhibitory functions have been reported in seafood protein.
대부분의 꽃게는 가공되지 않은 상태로 찜 또는 찜육 등 반가공 형태로 상업화하고 있다. 하지만 최근에 블루게 또는 머드 게 등으로부터 기능성 펩타이드 생산 및 특성을 밝히는 연구가 진행되어 오고 있다. 머드 게로부터 두 가지 종류의 항균활성 펩타이드의 분리 및 특성 연구가 발표되었다. 스노우 게의 가수분해물에서는 항암효과가 있는 분자량 228, 241, 291, 536 Da 펩타이드가 발표되었다. 대서양 바위게의 효소 가수분해물에서는 항균작용이 있는 펩타이드들이 분리 동정 되었는데 분자량은 200에서 750 Da이었다. 아미노산들의 구성은 주로 lysine, leucine, arginine, aspartic acid, glutamic acid 로 이루어졌다고 보고되었다. 블루 게에서도 역시 분자량이 3.7 kDa인 항균 펩타이드의 분리 및 특성에 대한 연구가 발표되었다.Most blue crabs are commercialized in semi-processed forms such as steamed or steamed meat in an unprocessed state. However, recent studies have been conducted to elucidate the production and characteristics of functional peptides from blue crabs or mud crabs. A study on the isolation and characterization of two types of antimicrobial active peptides from mud crab was published. In the hydrolyzate of snow crab, peptides with molecular weights of 228, 241, 291, and 536 Da with anticancer effect were announced. Antibacterial peptides were isolated and identified from the enzymatic hydrolyzate of Atlantic rock crab, and their molecular weights ranged from 200 to 750 Da. It was reported that the composition of amino acids was mainly composed of lysine, leucine, arginine, aspartic acid, and glutamic acid. A study on the isolation and characterization of an antibacterial peptide having a molecular weight of 3.7 kDa was also published in blue crab.
따라서, 생체에 안전하고, 유효성분이 안정하며, 무엇보다도 기존의 항산화 효과가 있는 물질보다 효과가 우수한 성분의 개발이 절실히 요망되고 있다.Therefore, there is an urgent need for the development of ingredients that are safe for the living body, have stable active ingredients, and above all, have excellent effects compared to substances having an existing antioxidant effect.
본 출원인은 꽃게 단백질 유래 펩타이드를 유효성분으로 함유하는 항산화 효능의 기능성 조성물 및 이의 제조방법을 출원한 바 있다.(대한민국공개특허공보 제10-2020-0012152호(2020.02.05.))The present applicant has applied for a functional composition with antioxidant efficacy containing a peptide derived from crab protein as an active ingredient and a method for manufacturing the same. (Korea Patent Publication No. 10-2020-0012152 (2020.02.05.))
상기 꽃게 단백질 유래 펩타이드를 유효성분으로 함유하는 조성물은 DPPH 라디컬 소거능이 우수하여 항산화 효과를 나타내며 세포에 대한 독성이 없어 항산화용 식품 조성물 등으로서 유용하게 사용될 수 있는 장점이 있지만, 면역증강효과가 부족한 단점이 있다.The composition containing the blue crab protein-derived peptide as an active ingredient has an excellent DPPH radical scavenging ability, exhibits an antioxidant effect, and has no toxicity to cells, so it has the advantage of being usefully used as a food composition for antioxidants, etc., but lacks an immune enhancing effect There are disadvantages.
본 발명의 목적은 항산화 효과가 우수할 뿐만 아니라 면역증강 효과도 우수한, 꽃게 유래 저분자 펩타이드를 함유하는 조성물을 제공하는 것이다.It is an object of the present invention to provide a composition containing a blue crab-derived low-molecular-weight peptide, which is excellent not only in the antioxidant effect but also in the immune-enhancing effect.
상기 목적을 달성하기 위하여 본 발명은 다음과 같은 수단을 제공한다.In order to achieve the above object, the present invention provides the following means.
본 발명은, 꽃게 단백질 유래 펩타이드 분말 60~70중량%, 흑삼 분말 10~20중량%, 아로니아 분말 10~20중량% 및 프로폴리스 분말 1~5중량%를 포함하되, 상기 꽃게 단백질 유래 펩타이드 분말은 꽃게의 껍질을 벗기지 않고 그대로 분쇄한 슬러리 100중량부에 단백질가수분해효소 0.1~1중량부를 가하고 50℃에서 2시간 동안 가수분해시켜 얻은 꽃게 단백질 가수분해물을 농축시킨 후 열풍건조하며, 상기 흑삼 분말은 흑삼 100중량부에 정제수 400~500중량부를 가하고 80~85℃에서 24~25시간 추출한 후에 여과하고 감압농축장치를 이용하여 50~80℃에서 농축한 다음, 농축액 100중량부에 덱스트린 20~30중량부 및 말토덱스트린 5~10중량부를 첨가하고 동결건조한 후 분말화하며, 상기 흑삼은 인삼을 105~110℃에서 2~3시간 동안 증숙한 후 70~75℃에서 1~2시간 동안 건조하는, 증숙 및 건조하는 과정을 4회 반복하는, 꽃게 유래 저분자 펩타이드를 함유하는 조성물을 제공한다.The present invention comprises 60 to 70% by weight of blue crab protein-derived peptide powder, 10 to 20% by weight of black ginseng powder, 10 to 20% by weight of aronia powder, and 1 to 5% by weight of propolis powder, wherein the blue crab protein-derived peptide powder 0.1 to 1 part by weight of a proteolytic enzyme is added to 100 parts by weight of the slurry pulverized without peeling of the silver crab, and the blue crab protein hydrolyzate obtained by hydrolysis at 50° C. for 2 hours is concentrated and dried with hot air, the black ginseng powder 400~500 parts by weight of purified water is added to 100 parts by weight of silver black ginseng, extracted at 80~85℃ for 24~25 hours, filtered, concentrated at 50~80℃ using a reduced pressure concentrator, and then dextrin 20~30 to 100 parts by weight of the concentrate After adding parts by weight and 5-10 parts by weight of maltodextrin, freeze-drying, and then powdering, the black ginseng is steamed at 105 to 110 ° C. for 2-3 hours and then dried at 70 to 75 ° C. for 1-2 hours. It provides a composition containing a low-molecular-weight peptide derived from blue crab, which repeats the process of steaming and drying four times.
상기 아로니아 분말은 아로니아 열매 100중량부에 쌀가루 15~20중량부를 가한 후 영하 20℃ ~ 영하 15℃에서 3~4일 동안 1차 숙성한 다음, 15~20℃에서 1~2일 동안 해동한 후에, 25~30℃에서 5~6일 동안 2차 숙성하고, 50~55℃에서 5~6시간 동안 건조한 후에 분말화한다.The aronia powder is first aged for 3-4 days at -20 ℃ ~ -15 ℃ after adding 15-20 parts by weight of rice flour to 100 parts by weight of aronia fruit, and then thawed at 15-20 ℃ for 1-2 days. After drying, it is secondarily aged at 25-30°C for 5-6 days, dried at 50-55°C for 5-6 hours, and then powdered.
상기 프로폴리스 분말은 프로폴리스 원괴 100중량부에 70%(v/v) 에탄올 500~600중량부 및 다공성 키토산 20~30중량부를 가하고 10~15℃에서 2~3시간 동안 교반하여 수득한 추출액을 동결건조하고 분말화하되, 상기 다공성 키토산은 키토산 용액 100중량부에 굴폐각 10~20중량부를 100~105℃에서 3~4시간 동안 교반한 후에 동결건조하며, 상기 키토산 용액은 5%의 아세트산 수용액 100중량부에 키토산 분말 20~30중량부를 용해시킨다. The propolis powder is an extract obtained by adding 500 to 600 parts by weight of 70% (v/v) ethanol and 20 to 30 parts by weight of porous chitosan to 100 parts by weight of the propolis mass and stirring at 10 to 15° C. for 2-3 hours. However, freeze-drying and powdering, the porous chitosan is freeze-dried after stirring 10-20 parts by weight of an oyster shell in 100 parts by weight of a chitosan solution at 100-105° C. for 3-4 hours, and the chitosan solution is a 5% aqueous acetic acid solution 20-30 parts by weight of chitosan powder is dissolved in 100 parts by weight.
본 발명에 따른 꽃게 유래 저분자 펩타이드를 함유하는 조성물은 항산화 효과가 우수할 뿐만 아니라 면역증강 효과도 우수한 장점이 있다. The composition containing the blue crab-derived low-molecular peptide according to the present invention has an advantage in that it has excellent antioxidative effect as well as an excellent immune enhancing effect.
이하, 본 발명을 상세히 설명하면 다음과 같다.Hereinafter, the present invention will be described in detail.
본 발명에서, 꽃게는 갑각류로서 우리나라에서 잡히는 매끈 꽃겟속, 주름 꽃겟속, 톱날 꽃겟속, 민 꽃겟속, 두갈래 민꽃겟속 등을 사용할 수 있으나, 이에 한정되는 것은 아니며, 상기 꽃겟속들 중에서 학명이 Ovalipes punctatus 인 깨다시 꽃게를 사용하는 것이 바람직하다.In the present invention, the blue crab can be used as a crustacean in Korea, such as smooth crab, wrinkled, saw bladed, and bifurcated, but is not limited thereto, and the scientific name among the above genera is It is preferable to use blue crab, Ovalipes punctatus.
본 발명에서 사용된 용어 항산화 효과는 산화반응을 억제하는 물질로서 활성산소 또는 자유라디칼이라고 하는 히드록실라디칼, 수퍼옥시드아니온, 과산화수소 등을 포함하는, 소위 산화물질의 작용을 소거 또는 감쇠시키는 물질을 총칭하는 의미로서, 항산화용 조성물, 산화방지제, 산화방지용 조성물 등의 용어와 동일한 의미로 사용된다.The term antioxidant effect used in the present invention refers to a substance that suppresses oxidation reaction, and a substance that eliminates or attenuates the action of so-called oxidizing substances, including hydroxyl radicals, superoxide anions, hydrogen peroxide, etc. called active oxygen or free radicals. As a generic meaning, it is used in the same meaning as terms such as antioxidant composition, antioxidant, and antioxidant composition.
먼저, 본 발명에 따른 꽃게 유래 저분자 펩타이드를 함유하는 조성물을 설명한다.First, a composition containing the blue crab-derived low molecular weight peptide according to the present invention will be described.
본 발명의 꽃게 유래 저분자 펩타이드를 함유하는 조성물은,The composition containing the low-molecular-weight peptide derived from blue crab of the present invention,
꽃게 단백질 유래 펩타이드 분말 60~70중량%, 흑삼 분말 10~20중량%, 아로니아 분말 10~20중량% 및 프로폴리스 분말 1~5중량%를 포함한다.Contains 60 to 70% by weight of blue crab protein-derived peptide powder, 10 to 20% by weight of black ginseng powder, 10 to 20% by weight of aronia powder, and 1 to 5% by weight of propolis powder.
상기 꽃게 단백질 유래 펩타이드 분말은 꽃게의 껍질을 벗기지 않고 그대로 분쇄한 슬러리 100중량부에 단백질가수분해효소 0.1~1중량부를 가하고 50℃에서 2시간 동안 가수분해시켜 얻은 꽃게 단백질 가수분해물을 농축시킨 후 열풍건조하여 수득한다.The blue crab protein-derived peptide powder is obtained by adding 0.1 to 1 part by weight of a proteolytic enzyme to 100 parts by weight of a slurry that has been pulverized without peeling the crab skin and hydrolyzed at 50° C. for 2 hours. After concentrating the blue crab protein hydrolyzate, hot air It is obtained by drying.
상기 단백질가수분해효소는 Bromelain을 사용하는 것이 바람직하다. 상기 Bromelain은 파인애플의 조직 안에 존재하는 단백질 가수분해효소로서 식품, 제약 및 화장품 원료제조 분야에 널리 시용되고 있다.It is preferable to use Bromelain as the proteolytic enzyme. The Bromelain is a proteolytic enzyme present in the tissues of pineapple and is widely used in food, pharmaceutical and cosmetic raw material manufacturing fields.
본 발명의 꽃게 단백질 유래 펩타이드 분말은 DPPH 라디컬을 소거함으로써 우수한 항산화 효과를 나타낼 수 있다.The blue crab protein-derived peptide powder of the present invention can exhibit excellent antioxidant effect by scavenging DPPH radicals.
상기 흑삼은 홍삼의 주성분인 진세노사이드 Rg3 성분이 증가하여 인삼과 홍삼보다 콜레스테롤 조절 능력이 더 뛰어나며, 현대 의학에서 난치성으로 분류하고 있는 성인병에 대한 예방효과가 매우 뛰어난 것이 증명되고 발표되고 있으며, 이외에도 사포닌의 흡수율이 홍삼에 비해 월등히 높고 기억력 개선과 피로회복 등의 다양한 효능 가진다. The black ginseng has a higher cholesterol control ability than ginseng and red ginseng by increasing the ginsenoside Rg3 component, which is the main component of red ginseng, and has been proven and announced that it is very effective in preventing adult diseases classified as intractable in modern medicine. The absorption rate of saponin is much higher than that of red ginseng, and it has various effects such as improving memory and recovering from fatigue.
상기 흑삼 분말은 흑삼 100중량부에 정제수 400~500중량부를 가하고 80~85℃에서 24~25시간 추출한 후에 여과하고 감압농축장치를 이용하여 50~80℃에서 농축한 다음, 농축액 100중량부에 덱스트린 20~30중량부 및 말토덱스트린 5~10중량부를 첨가하고 동결건조한 후 분말화한다.The black ginseng powder is obtained by adding 400 to 500 parts by weight of purified water to 100 parts by weight of black ginseng, extracting it at 80 to 85° C. for 24 to 25 hours, filtering it, and then concentrating at 50 to 80° C. using a reduced pressure concentrator, and then dextrin to 100 parts by weight of the concentrate. 20-30 parts by weight and 5-10 parts by weight of maltodextrin are added, freeze-dried, and then powdered.
상기 흑삼은 인삼을 105~110℃에서 2~3시간 동안 증숙한 후 70~75℃에서 1~2시간 동안 건조하는, 증숙 및 건조하는 과정을 4회 반복하여 수득한다.The black ginseng is obtained by repeating the process of steaming and drying ginseng at 105~110℃ for 2-3 hours and then drying it at 70~75℃ for 1-2 hours.
상기 아로니아(Aronia melanocarpa)는 블랙 초크베리(black chokeberry) 라고도 알려져 있으며, 그 원산지는 북부 아메리카지만 현재 유럽에서도 인기를 끌고 있으며 국내에서도 관심이 높아지며 재배되고 있다. 색과 향이 좋아 잼, 와인, 주스, 차 등 다양한 식자재로서의 이용 가치가 높고, 아로니아에 포함되어있는 다량의 안토시아닌은 짙은 자줏빛을 띠며 항산화물질, 천연색소로의 사용 가능하고, 그밖에 페놀산류, 플라보노이드류와 같은 생리활성물질은 항산화 효과, 항염증 효과, 항당뇨 효과, 면역조절기능 등 다양한 기능을 가지는 것으로 알려져 있다.The Aronia ( Aronia melanocarpa ) is also known as black chokeberry, its origin is North America, but it is currently popular in Europe and is being cultivated with increasing interest in Korea. Due to its good color and fragrance, it is highly valuable as a variety of food materials such as jam, wine, juice, and tea. The large amount of anthocyanins contained in aronia has a dark purple color and can be used as an antioxidant and natural colorant. In addition, phenolic acids and flavonoids Such physiologically active substances are known to have various functions such as antioxidant effect, anti-inflammatory effect, anti-diabetic effect, and immunomodulatory function.
상기 아로니아 분말은 아로니아 열매 100중량부에 쌀가루 15~20중량부를 가한 후 영하 20℃ ~ 영하 15℃에서 3~4일 동안 1차 숙성한 다음, 15~20℃에서 1~2일 동안 해동한 후에, 25~30℃에서 5~6일 동안 2차 숙성하고, 50~55℃에서 5~6시간 동안 건조한 후에 분말화한다. The aronia powder is first aged for 3-4 days at -20 ℃ ~ -15 ℃ after adding 15-20 parts by weight of rice flour to 100 parts by weight of aronia fruit, and then thawed at 15-20 ℃ for 1-2 days. After drying, it is secondarily aged at 25-30°C for 5-6 days, dried at 50-55°C for 5-6 hours, and then powdered.
본 발명에서는 아로니아 열매를 상기와 같이 제조하여 아로니아 열매의 떫은맛을 제거한 장점이 있다.In the present invention, there is an advantage in that the astringent taste of the aronia fruit is removed by preparing the aronia fruit as described above.
상기 프로폴리스는 벌집에서 얻어지는 지용성 복합체로 식물이 분비하는 왁스와 수지 물질을 모아 벌 자신의 침샘 분비물과 혼합하여 만든 수지성, 점착성, 고무상의 물질로 벌집 출입구에 발라 외부로부터 균의 유입을 막기 위해 사용하며, 주로 벌통 내의 표면틈새에 발라 빗물 및 냉기를 막았으며 벌집 전체 구조를 강화하기 위해 사용한다. 일반적인 성분으로는 수지 50~55%, 밀랍 30%, 정유 8~10%, 회분 5%, 각종 유기성분, 미네랄 등의 성분으로 이루어져있으며, 구성 성분은 약 150~180개의 휘발성 물질과 페놀계 화합물로 알려져 있다. 프로폴리스는 플라보노이드와 유기물이 다량 함유되어 있으며 성분 중 플라보노이드는 강력한 항균 효과를 가지는 것으로 보고되고 있다. 프로폴리스에 포함된 계피산과 카페릭산 에스터를 비롯한 많은 종류의 플라보노이드 성분은 항암, 항균, 항박테리아, 항바이러스, 항염증, 항진균성, 항산화 및 알레르기성 피부염 치료에 도움이 되는 생리활성 기작을 나타내는 것으로 보고되어 있다.The propolis is a fat-soluble complex obtained from the honeycomb. It is a resinous, sticky, rubbery substance made by collecting wax and resinous substances secreted by plants and mixing them with the bees' own salivary gland secretions. It is mainly applied to the surface crevices in the hive to prevent rainwater and cold air, and is used to strengthen the overall structure of the hive. Common ingredients are 50-55% resin, 30% beeswax, 8-10% essential oil, 5% ash, various organic ingredients, minerals, etc. The composition consists of about 150-180 volatile substances and phenolic compounds is known as Propolis contains a large amount of flavonoids and organic matter, and it is reported that flavonoids among the ingredients have a strong antibacterial effect. Many kinds of flavonoids, including cinnamic acid and caffeic acid esters contained in propolis, exhibit anticancer, antibacterial, antibacterial, antiviral, anti-inflammatory, antifungal, antioxidant and physiological activity mechanisms that are helpful in the treatment of allergic dermatitis. has been reported
상기 프로폴리스 분말은 프로폴리스 원괴 100중량부에 70%(v/v) 에탄올 500~600중량부 및 다공성 키토산 20~30중량부를 가하고 10~15℃에서 2~3시간 동안 교반하여 수득한 추출액을 동결건조하고 분말화한다. The propolis powder is an extract obtained by adding 500 to 600 parts by weight of 70% (v/v) ethanol and 20 to 30 parts by weight of porous chitosan to 100 parts by weight of the propolis mass and stirring at 10 to 15° C. for 2-3 hours. Freeze-dried and powdered.
상기 다공성 키토산은 키토산 용액 100중량부에 굴폐각 10~20중량부를 100~105℃에서 3~4시간 동안 교반한 후에 동결건조하여 제조한다.The porous chitosan is prepared by stirring 10 to 20 parts by weight of an oyster shell in 100 parts by weight of a chitosan solution at 100 to 105° C. for 3 to 4 hours and then freeze-drying.
상기 키토산 용액은 5%의 아세트산 수용액 100중량부에 키토산 분말 20~30중량부를 용해시켜 제조한다.The chitosan solution is prepared by dissolving 20 to 30 parts by weight of chitosan powder in 100 parts by weight of a 5% aqueous acetic acid solution.
본 발명에서는 프로폴리스 분말을 상기와 같이 제조하여 프로폴리스의 불쾌한 향을 제거한 장점이 있다.In the present invention, there is an advantage in that the unpleasant odor of propolis is removed by preparing the propolis powder as described above.
본 발명에 따른 꽃게 유래 저분자 펩타이드를 함유하는 조성물은 항산화 효과가 우수할 뿐만 아니라 면역증강 효과도 우수한 장점이 있다. The composition containing the blue crab-derived low-molecular peptide according to the present invention has an advantage in that it has excellent antioxidative effect as well as an excellent immune enhancing effect.
본 발명에 따른 꽃게 유래 저분자 펩타이드를 함유하는 조성물은 물에 용해하여 섭취할 수도 있고, 식품에 첨가하여 섭취할 수도 있다.The composition containing the blue crab-derived low-molecular peptide according to the present invention may be ingested by dissolving it in water, or it may be ingested by adding it to food.
이하, 실시 예를 통하여 본 발명의 구성 및 효과를 더욱 상세히 설명하고자 한다. 이들 실시 예는 오로지 본 발명을 예시하기 위한 것일 뿐 본 발명의 범위가 이들 실시 예에 의해 제한되는 것은 아니다. Hereinafter, the configuration and effects of the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and the scope of the present invention is not limited by these examples.
꽃게 단백질 유래 펩타이드 분말 65중량%, 흑삼 분말 15중량%, 아로니아 분말 15중량% 및 프로폴리스 분말 5중량%를 혼합하여 꽃게 유래 저분자 펩타이드를 함유하는 조성물을 제조하였다.65% by weight of blue crab protein-derived peptide powder, 15% by weight of black ginseng powder, 15% by weight of aronia powder, and 5% by weight of propolis powder were mixed to prepare a composition containing blue crab-derived low molecular weight peptide.
상기 꽃게 단백질 유래 펩타이드 분말은, 꽃게의 껍질을 벗기지 않고 그대로 분쇄한 슬러리 1.5ton에 Bromelain 4.8㎏을 가하고 50℃에서 2시간 동안 가수분해시킨 꽃게 단백질 가수분해물을 30Brix로 농축시킨 후 열풍건조하여 제조하였다.The blue crab protein-derived peptide powder was prepared by adding 4.8 kg of bromelain to 1.5 tons of slurry pulverized without peeling the crab skin, and concentrating the blue crab protein hydrolyzate hydrolyzed at 50° C. for 2 hours with 30Brix, followed by hot air drying. .
상기 흑삼 분말은 흑삼 100중량부에 정제수 400중량부를 가하고 80℃에서 24시간 추출한 후에 여과하고 감압농축장치를 이용하여 80℃에서 농축한 농축액 100중량부에 덱스트린 20중량부 및 말토덱스트린 5중량부를 첨가하고 동결건조한 후 분말화하여 제조하였다. 상기 흑삼은 인삼을 110℃에서 2시간 동안 증숙한 후 70℃에서 1시간 동안 건조하는, 증숙 및 건조하는 과정을 4회 반복하여 제조하였다. The black ginseng powder is obtained by adding 400 parts by weight of purified water to 100 parts by weight of black ginseng, extracting at 80° C. for 24 hours, filtering, and adding 20 parts by weight of dextrin and 5 parts by weight of maltodextrin to 100 parts by weight of a concentrate concentrated at 80° C. using a reduced pressure concentrator. and lyophilized, and then powdered. The black ginseng was prepared by repeating the process of steaming and drying ginseng at 110° C. for 2 hours and then drying it at 70° C. for 1 hour.
상기 아로니아 분말은 아로니아 열매 100중량부에 쌀가루 15중량부를 가한 후 영하 15℃에서 3일 동안 1차 숙성한 다음, 20℃에서 1일 동안 해동한 후에, 25℃에서 6일 동안 2차 숙성하고, 55℃에서 6시간 동안 건조한 후에 분말화하여 제조하였다. After adding 15 parts by weight of rice flour to 100 parts by weight of aronia fruit, the aronia powder was first aged for 3 days at -15°C below zero, then thawed at 20°C for 1 day, and then secondary aged at 25°C for 6 days. and dried at 55° C. for 6 hours, and then powdered.
상기 프로폴리스 분말은 프로폴리스 원괴 100중량부에 70%(v/v) 에탄올 500중량부 및 다공성 키토산 30중량부를 가하고 15℃에서 2시간 동안 교반하여 수득한 추출액을 동결건조하고 분말화하여 제조하였다. 상기 다공성 키토산은 키토산 용액 100중량부에 굴폐각 20중량부를 105℃에서 4시간 동안 교반한 후에 동결건조하여 제조하였다. 상기 키토산 용액은 5%의 아세트산 수용액 100중량부에 키토산 분말 30중량부를 용해시켜 제조하였다.The propolis powder was prepared by adding 500 parts by weight of 70% (v/v) ethanol and 30 parts by weight of porous chitosan to 100 parts by weight of the propolis original mass and stirring at 15° C. for 2 hours. The resulting extract was freeze-dried and powdered. . The porous chitosan was prepared by stirring 20 parts by weight of an oyster shell in 100 parts by weight of a chitosan solution at 105° C. for 4 hours and then freeze-drying. The chitosan solution was prepared by dissolving 30 parts by weight of chitosan powder in 100 parts by weight of a 5% aqueous acetic acid solution.
[비교예 1][Comparative Example 1]
흑삼 원료를 분쇄하여 100메쉬 체를 통과한 분말 시료:정제수를 375g:1,875g의 비율로 혼합하였다. 그 후 121℃ 살균솥에서 2시간 동안 팽윤 및 살균과정을 거친 뒤, 교반기로 교반하면서 50℃까지 방냉하였다. NaHCO3를 이용하여 방냉한 시료를 pH 4.8로 조정한 후, 펙티네이즈와 셀룰레이즈를 각각 기질 대비 3%(w/w)씩 첨가하여 잘 혼합한 다음 50℃에서 3시간 동안 효소처리를 하였다. 효소처리한 시료는 α-아밀라아제를 기질 대비 2% 첨가하여 잘 혼합한 다음, 90~95℃에서 3시간 동안 가수분해시켰다. α-아밀라아제 처리가 완료된 뒤, 진공동결 건조한 분말화하여 피로회복증진용 조성물을 제조하였다.Black ginseng raw material was pulverized and powder sample passed through a 100 mesh sieve: purified water was mixed in a ratio of 375 g: 1,875 g. Then, after swelling and sterilization process for 2 hours in a 121°C sterilization pot, the mixture was allowed to cool to 50°C while stirring with a stirrer. After adjusting the pH of the sample cooled with NaHCO 3 to 4.8, pectinase and cellulase were added at a rate of 3% (w/w) relative to the substrate, respectively, and mixed well, followed by enzymatic treatment at 50° C. for 3 hours. The enzyme-treated sample was mixed well by adding 2% of α-amylase to the substrate, and then hydrolyzed at 90-95° C. for 3 hours. After the α-amylase treatment was completed, vacuum freeze-dried powder was used to prepare a composition for improving fatigue recovery.
[비교예 2][Comparative Example 2]
대황 추출물 및 신선초 추출물을 중량비 1:1의 비율로 혼합하여 피로회복용 조성물을 제조하였다. 상기 대황 추출물은 대황 전초 10kg을 에탄올 30L에 넣고 80℃에서 3시간 동안 추출하였다. 상기 신선초 추출물은 신선초 전초 10kg을 에탄올 30L에 넣고 80℃에서 3시간 동안 추출하였다. 상기 추출물은 상온에서 여과지에 여과하고 추출액의 에탄올이 완전히 증발할 때까지 감압 하에 농축기를 사용하여 농축하여 사용하였다.A composition for recovery from fatigue was prepared by mixing rhubarb extract and fresh herb extract in a weight ratio of 1:1. The rhubarb extract was extracted by putting 10 kg of rhubarb whole plant in 30 L of ethanol and at 80° C. for 3 hours. The extract was put into 30L of ethanol and 10 kg of fresh herb extract was extracted at 80 ℃ for 3 hours. The extract was filtered on a filter paper at room temperature, and concentrated using a concentrator under reduced pressure until the ethanol of the extract was completely evaporated.
[비교예 3][Comparative Example 3]
물 100 중량부에 대하여, 세척한 장어 70 중량부, 무 10 중량부, 건 홍고추 3 중량부, 마늘 3 중량부, 건 표고버섯 10 중량부, 생강 2 중량부, 양파 5 중량부, 검은콩 7 중량부, 건매실 1 중량부, 소주 0.5 중량부, 된장물 1 중량부, 곶감 2 중량부, 잣 1 중량부를 용기에 투입한 후 15분간 100℃ 이상의 온도에서 끓이고 이후 30분간 60℃의 온도에서 끓이고, 끓여서 얻은 혼합물을 일정 시간동안 식힌 후 분쇄기에 넣고 분쇄한 다음 분쇄물을 체로 여과시켜 액상 원액을 추출하여 장어를 주원료로 한 피로회복용 조성물을 제조하였다.Based on 100 parts by weight of water, washed eel 70 parts by weight, radish 10 parts by weight, dried red pepper 3 parts by weight, garlic 3 parts by weight, dried shiitake mushrooms 10 parts by weight, ginger 2 parts by weight, onion 5 parts by weight, black beans 7 After putting 1 part by weight, 1 part by weight of dried plum, 0.5 parts by weight of soju, 1 part by weight of soybean paste, 2 parts by weight of dried persimmon, and 1 part by weight of pine nuts in the container, boil at a temperature of 100 ° C or higher for 15 minutes, and then at a temperature of 60 ° C for 30 minutes. After the mixture obtained by boiling and boiling was cooled for a certain period of time, it was put into a grinder and pulverized, and the pulverized product was filtered through a sieve to extract a liquid stock solution to prepare a composition for fatigue recovery using eel as the main raw material.
[실험예 1][Experimental Example 1]
상기 실시예 1에서 제조된 꽃게 유래 저분자 펩타이드를 함유하는 조성물과 비교예 1 내지 3에서 제조된 피로회복용 조성물에 대하여 관능검사를 실시하여 비교하였다. 관능검사는 기능성식품을 주로 섭취하는 대상자를 고려하여 남녀 성별 구분없이 50~70대 성인 및 노인을 대상으로 총 40명을 선발하였으며, 물 100㎖에 상기 실시예 1에서 제조한 꽃게 유래 저분자 펩타이드를 함유하는 조성물 10g을 혼합하여 섭취하게 하였고, 물 100㎖에 상기 비교예 1에서 제조한 피로회복증진용 조성물 10g을 혼합하여 섭취하게 하였고, 비교예 2의 피로회복용 조성물 100㎖을 섭취하게 하였고, 비교예 3의 피로회복용 조성물 100㎖을 섭취하게 하였으며, 냄새, 맛, 색상 및 전체적 기호도 평가를 10점 척도법을 이용하여 실시하였으며, 그 결과를 평균하여 표 1에 나타내었다. (10점 : 매우 우수, 7점 : 우수, 5점 : 보통, 1점 : 미흡)The composition containing the blue crab-derived low-molecular peptide prepared in Example 1 and the composition for recovering fatigue prepared in Comparative Examples 1 to 3 were compared by performing a sensory test. For the sensory test, a total of 40 people were selected from adults and the elderly in their 50s to 70s regardless of gender, considering subjects who mainly consume functional foods. 10 g of the composition containing the composition was mixed and ingested, 10 g of the composition for improving fatigue recovery prepared in Comparative Example 1 was mixed with 100 ml of water and ingested, and 100 ml of the composition for fatigue recovery of Comparative Example 2 was ingested, and compared 100 ml of the composition for recovery from fatigue of Example 3 was ingested, and evaluation of smell, taste, color, and overall preference was performed using a 10-point scale method, and the results were averaged and shown in Table 1. (10 points: very good, 7 points: excellent, 5 points: average, 1 point: poor)
표 1의 결과에서와 같이, 실시예 1에서 제조된 꽃게 유래 저분자 펩타이드를 함유하는 조성물은 비교예 1 내지 3에서 제조된 피로회복용 조성물에 비하여 냄새, 맛, 색상 및 전체적 기호도가 우수한 것을 알 수 있다.As shown in the results in Table 1, it can be seen that the composition containing the blue crab-derived low-molecular peptide prepared in Example 1 has superior odor, taste, color and overall preference compared to the fatigue recovery composition prepared in Comparative Examples 1 to 3. .
[실험예 2][Experimental Example 2]
상기 실험예 1의 섭취 대상자를 대상으로, 10명에게는 물 100㎖에 상기 실시예 1에서 제조한 꽃게 유래 저분자 펩타이드를 함유하는 조성물 10g을 혼합하여 1주일 동안 섭취하게 하였고, 다른 10명에게는 물 100㎖에 상기 비교예 1에서 제조한 피로회복증진용 조성물 10g을 혼합하여 1주일 동안 섭취하게 하였고, 또 다른 10명에게는 비교예 2의 피로회복용 조성물 100㎖을 1주일 동안 섭취하게 하였고, 또 다른 10명에게는 비교예 3의 피로회복용 조성물 100㎖을 1주일 동안 섭취하게 하였다. 1주일 동안 섭취한 후에 피로회복 효과에 대한 설문조사를 실시하였으며, 그 결과를 표 2에 나타내었다.For the subjects of ingestion of Experimental Example 1, 10 people mixed 10 g of the composition containing the blue crab-derived low-molecular peptide prepared in Example 1 in 100 ml of water and ingested for 1 week, and the other 10 people were given 100 ml of water. In ml, 10 g of the composition for improving fatigue recovery prepared in Comparative Example 1 was mixed and ingested for 1 week, and another 10 people were allowed to ingest 100 ml of the composition for recovery from fatigue of Comparative Example 2 for 1 week, and another 10 People were given 100 ml of the composition for recovery from fatigue of Comparative Example 3 for 1 week. After taking it for 1 week, a survey was conducted on the fatigue recovery effect, and the results are shown in Table 2.
효과여부recovery from fatigue
Effectiveness
표 2의 결과에서와 같이, 실시예 1에서 제조된 꽃게 유래 저분자 펩타이드를 함유하는 조성물은 비교예 1 내지 3에서 제조된 피로회복용 조성물에 비하여 피로회복 효과가 우수한 것을 확인할 수 있다.As shown in the results of Table 2, it can be confirmed that the composition containing the blue crab-derived low molecular weight peptide prepared in Example 1 has an excellent fatigue recovery effect compared to the fatigue recovery composition prepared in Comparative Examples 1 to 3.
Claims (3)
상기 꽃게 단백질 유래 펩타이드 분말은 꽃게의 껍질을 벗기지 않고 그대로 분쇄한 슬러리 100중량부에 단백질가수분해효소 0.1~1중량부를 가하고 50℃에서 2시간 동안 가수분해시켜 얻은 꽃게 단백질 가수분해물을 농축시킨 후 열풍건조하며,
상기 흑삼 분말은 흑삼 100중량부에 정제수 400~500중량부를 가하고 80~85℃에서 24~25시간 추출한 후에 여과하고 감압농축장치를 이용하여 50~80℃에서 농축한 다음, 농축액 100중량부에 덱스트린 20~30중량부 및 말토덱스트린 5~10중량부를 첨가하고 동결건조한 후 분말화하며,
상기 흑삼은 인삼을 105~110℃에서 2~3시간 동안 증숙한 후 70~75℃에서 1~2시간 동안 건조하는, 증숙 및 건조하는 과정을 4회 반복하는,
꽃게 유래 저분자 펩타이드를 함유하는 조성물.
Contains 60 to 70% by weight of blue crab protein-derived peptide powder, 10 to 20% by weight of black ginseng powder, 10 to 20% by weight of aronia powder, and 1 to 5% by weight of propolis powder,
The blue crab protein-derived peptide powder is obtained by adding 0.1 to 1 part by weight of a proteolytic enzyme to 100 parts by weight of a slurry that has been pulverized without peeling the crab skin and hydrolyzed at 50° C. for 2 hours. dry,
The black ginseng powder is obtained by adding 400 to 500 parts by weight of purified water to 100 parts by weight of black ginseng, extracting it at 80 to 85° C. for 24 to 25 hours, filtering, and using a reduced pressure concentrator to concentrate at 50 to 80° C., and then dextrin to 100 parts by weight of the concentrate. 20-30 parts by weight and 5-10 parts by weight of maltodextrin are added, lyophilized, and then powdered,
The black ginseng is steamed for 2-3 hours at 105 ~ 110 ℃ ginseng, then dried at 70 ~ 75 ℃ for 1 ~ 2 hours, repeating the process of steaming and drying 4 times,
A composition containing a low-molecular-weight peptide derived from blue crab.
상기 아로니아 분말은 아로니아 열매 100중량부에 쌀가루 15~20중량부를 가한 후 영하 20℃ ~ 영하 15℃에서 3~4일 동안 1차 숙성한 다음, 15~20℃에서 1~2일 동안 해동한 후에, 25~30℃에서 5~6일 동안 2차 숙성하고, 50~55℃에서 5~6시간 동안 건조한 후에 분말화하는,
꽃게 유래 저분자 펩타이드를 함유하는 조성물.
The method of claim 1,
The aronia powder is first aged for 3-4 days at -20 ℃ ~ -15 ℃ after adding 15-20 parts by weight of rice flour to 100 parts by weight of aronia fruit, and then thawed at 15-20 ℃ for 1-2 days. After drying, secondary aging for 5-6 days at 25-30℃, drying at 50-55℃ for 5-6 hours, and powdering,
A composition containing a low-molecular-weight peptide derived from blue crab.
상기 프로폴리스 분말은 프로폴리스 원괴 100중량부에 70%(v/v) 에탄올 500~600중량부 및 다공성 키토산 20~30중량부를 가하고 10~15℃에서 2~3시간 동안 교반하여 수득한 추출액을 동결건조하고 분말화하되,
상기 다공성 키토산은 키토산 용액 100중량부에 굴폐각 10~20중량부를 100~105℃에서 3~4시간 동안 교반한 후에 동결건조하며,
상기 키토산 용액은 5%의 아세트산 수용액 100중량부에 키토산 분말 20~30중량부를 용해시키는,
꽃게 유래 저분자 펩타이드를 함유하는 조성물.The method of claim 1,
The propolis powder is an extract obtained by adding 500 to 600 parts by weight of 70% (v/v) ethanol and 20 to 30 parts by weight of porous chitosan to 100 parts by weight of a propolis mass and stirring at 10 to 15° C. for 2-3 hours. Freeze-dried and powdered,
The porous chitosan is freeze-dried after stirring 10 to 20 parts by weight of an oyster shell in 100 parts by weight of a chitosan solution at 100 to 105° C. for 3 to 4 hours,
The chitosan solution is to dissolve 20 to 30 parts by weight of chitosan powder in 100 parts by weight of a 5% aqueous acetic acid solution,
A composition containing a low-molecular-weight peptide derived from blue crab.
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| KR1020200147606A KR102546027B1 (en) | 2020-11-06 | 2020-11-06 | Comprising low molecular weight Peptide from Swimming Crab |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007053912A (en) * | 2005-08-22 | 2007-03-08 | Api Co Ltd | Method for producing propolis powder |
| KR20130109502A (en) * | 2012-03-27 | 2013-10-08 | (주)아모레퍼시픽 | Particles of concentrated plants |
| KR20170125191A (en) * | 2016-05-04 | 2017-11-14 | 농업회사법인 써니 (주) | Pill using aronia berry and manufacturing method thereof |
| KR20200012152A (en) | 2018-07-26 | 2020-02-05 | 유선균 | Peptides Production from Protein of Crab and Antioxidant Composition Comprising Peptides |
-
2020
- 2020-11-06 KR KR1020200147606A patent/KR102546027B1/en active IP Right Grant
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007053912A (en) * | 2005-08-22 | 2007-03-08 | Api Co Ltd | Method for producing propolis powder |
| KR20130109502A (en) * | 2012-03-27 | 2013-10-08 | (주)아모레퍼시픽 | Particles of concentrated plants |
| KR20170125191A (en) * | 2016-05-04 | 2017-11-14 | 농업회사법인 써니 (주) | Pill using aronia berry and manufacturing method thereof |
| KR20200012152A (en) | 2018-07-26 | 2020-02-05 | 유선균 | Peptides Production from Protein of Crab and Antioxidant Composition Comprising Peptides |
Non-Patent Citations (1)
| Title |
|---|
| Antunes-Valcareggi S. A. et al, Enzymatic Hydrolysis of Blue Crab (Callinectes Sapidus) Waste Processing to Obtain Chitin, Protein, and Astaxanthin-Enriched Extract, IJOEAR 3(1):pp.81-92 (2017. 1.)] * |
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| KR102546027B1 (en) | 2023-06-26 |
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