KR20220052125A - Feed composition for reducing odor comprising microorganisms of Lactococcus sp. - Google Patents
Feed composition for reducing odor comprising microorganisms of Lactococcus sp. Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/46—Streptococcus ; Enterococcus; Lactococcus
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Abstract
Description
본 발명은 락토코커스 속 미생물을 포함하는 악취 저감용 조성물에 관한 것으로, 바람직하게는 동애등에 사육환경에서 발생되는 악취를 저감할 수 있는 락토코커스 속 미생물을 포함하는 악취 저감용 사료 조성물, 동애등에를 이용한 유기성 페기물 처리에서의 악취 저감을 위한 락토코커스 속 미생물을 포함하는 악취 저감용 조성물에 관한 것이다. The present invention relates to a composition for reducing odors containing microorganisms of the genus Lactococcus, and preferably a feed composition for reducing odors containing microorganisms of the genus Lactococcus, which can reduce odors generated in a breeding environment such as Dongae, etc. It relates to a composition for reducing odor containing microorganisms of the genus Lactococcus for reducing odor in the used organic waste treatment.
동애등에는 파리목 동애등에 과에 속하는 곤충으로 한국, 일본, 중국, 대만 등 동아시아와 미국 등지에 널리 분포되어 있다. 년 3세대 이상 발생하며, 첫 성충 출현은 6월경에 발생하여 10월경까지 나타난다. 이들은 고치 형태의 번데기를 만들지 않고 종령 유충 형태 그대로 번데기가 되어 월동한다.Dongae is an insect belonging to the family Diptera, and it is widely distributed in East Asia such as Korea, Japan, China, Taiwan, etc. and the United States. It occurs more than 3 generations per year, and the first imago appears around June and appears until around October. They do not make cocoon-shaped pupae, but pupate and overwinter in the form of larval larvae.
동애등에는 대표적인 환경정화 곤충으로 유충시기에만 먹이활동을 하며, 성충 시기에는 먹이활동을 하지 않고, 축사나 생활쓰레기 및 음식물쓰레기와 같은 유기성 폐기물이 야적되어 있는 곳에서 생활사를 이어가는 특징이 있어 익충으로 분류되고 있다. 동애등에의 유충은 잡식성으로 유기 폐기물을 섭식하면서 50-60℃의 고온을 발생시키고, 항생 물질을 분비하여 타 생명체의 접근을 억제하는 것으로 알려져 있으며(Can. J. Microbiol. 6, 115-119), 이러한 특성을 이용하여 최근 동애등에를 이용하여 청결하고 효과적이면서 친환경적으로 남은 음식물 및 가축분뇨 등 유기성 폐기물을 처리하는 방법에 이용되고 있다. Dongae is a representative environmental purification insect that feeds only during the larval period, does not feed during the adult period, and continues its life cycle in places where organic wastes such as livestock and household waste and food waste are stored. are being classified. The larvae of the larvae are omnivorous and are known to generate a high temperature of 50-60℃ while feeding on organic waste and to suppress the access of other organisms by secreting antibiotics (Can. J. Microbiol. 6, 115-119). , using these characteristics, it is recently used in a method of treating organic waste such as leftover food and livestock manure in a clean, effective and eco-friendly way using Dongae.
뿐만 아니라, 동애등에 유충이 분비하는 다양한 항생물질은 의약산업에서도 주목을 받고 있으며, 동애등에 유충은 단백질 함량이 전체의 40% 이상이고, 지질 함량이 전체의 30% 이상으로 영양학적으로 매우 우수하므로 가축의 양질 사료로 사용되고 있으며(J Anim Sci 44, 395-400), 높은 지질 함량은 바이오 연료로서의 가능성도 제시되고 있다(Waste Manag. Res. 27, 603-610). In addition, various antibiotics secreted by the larvae of the larvae of Dongae, etc. are attracting attention in the pharmaceutical industry. It is used as a quality feed for livestock (J Anim Sci 44, 395-400), and its high lipid content is also suggested as a biofuel (Waste Manag. Res. 27, 603-610).
생존 경쟁이 치열한 자연계에서는 알과 유충이 원활하게 공급되기 어려우므로 동애등에 유충의 사육에 대한 관심이 증가되고 있으며, 다양한 방법으로 동애등에를 대량생산하는 방법이 고안되었다. 동애등에 유충의 생육과정에서는 유충 분해에 따른 악취가 동반되므로 악취를 저감할 수 있으면서, 동애등에의 성장은 향상시키고, 사육장의 작업환경을 개선할 수 있는 방법이 요구된다. In the natural world where competition for survival is fierce, it is difficult to supply eggs and larvae smoothly, so interest in breeding larvae of larvae is increasing, and methods for mass production of larvae in various methods have been devised. In the growth process of larvae of larvae, there is a need for a method that can reduce odor while improving the growth of larvae and improving the working environment of the kennel.
본 발명이 해결하고자 하는 과제는 락토코커스 플란타룸 균주 (기탁번호 KACC 13445) 또는 이의 배양액을 포함하는 사료 조성물을 제공하는데 있다. An object of the present invention is to provide a feed composition comprising a Lactococcus plantarum strain (Accession No. KACC 13445) or a culture solution thereof.
본 발명이 해결하고자 하는 다른 과제는 락토코커스 플란타룸 균주 (기탁번호 KACC 13445) 또는 이의 배양액을 포함하는 조성물을 이용한 동애등에의 사육에서 악취를 저감시키는 방법을 제공하는데 있다.Another object to be solved by the present invention is to provide a method for reducing odor in rearing of Dongae using a composition comprising a Lactococcus plantarum strain (Accession No. KACC 13445) or a culture solution thereof.
본 발명이 해결하고자 하는 또 다른 과제는 락토코커스 플란타룸 (Lactococcus plantarum) 균주 (기탁번호 KACC 13445) 또는 이의 배양액을 포함하는 조성물을 이용하는, 동애등에를 이용한 유기성 폐기물 처리에서의 악취저감 방법 및 유기성 폐기물 처리 속도의 향상 방법을 제공하는데 있다. Another problem to be solved by the present invention is a method for reducing odor in organic waste treatment using Lactococcus plantarum strain (Accession No. KACC 13445) or a composition comprising a culture solution thereof, and organic waste treatment using Dongae, etc. An object of the present invention is to provide a method for improving the speed of waste disposal.
상기 과제를 해결하기 위하여 본 발명은 락토코커스 플란타룸 (Lactococcus plantarum) 균주 (기탁번호 KACC 13445) 또는 이의 배양액을 포함하는 사료 조성물을 제공한다. In order to solve the above problems, the present invention provides a feed composition comprising a Lactococcus plantarum strain (Accession No. KACC 13445) or a culture solution thereof.
본 발명에 의하면, 상기 사료 조성물은 곤충 유충의 생육증진 및 사육환경에서 발생될 수 있는 악취를 저감할 수 있다. According to the present invention, the feed composition can promote the growth of insect larvae and reduce odors that may be generated in a breeding environment.
본 발명에 의하면, 상기 곤충 유충은 특별히 제한이 없으나, 바람직하게는 동애등에 유충일 수 있다. According to the present invention, the insect larvae are not particularly limited, but may preferably be larvae such as Dongae.
본 발명에 의하면 상기 동애등에는 동에등에 과에 속하는 것이면 제한이 없으며, 예를 들어, 동애등에(Ptecticus tenebrifer), 아메리카동에등에(Hermetia illucens), 검정꼬마동애등에(Allognosta vagans), 꼬마동에등에(Microchrysa flaviventris), 방울동애등에 (Craspedometopon frontale), 범동애등에(Odontomyia garatas) 누런얼룩동애등에(Ptecticus aurifer)일 수 있고, 바람직하게는 동애등에(Ptecticus tenebrifer) 또는 아메리카동에등에(Hermetia illucens)일 수 있다. According to the present invention, the Dongae, etc. is limited as long as it belongs to the family Dongae, etc. No, for example, in Dongae ( Ptecticus) tenebrifer ), on the American guinea pig ( Hermetia illucens ), on the black guinea pig ( Allognosta ) vagans ), on the larvae ( Microchrysa flaviventris ), on the larvae ( Craspedometopon ) frontale ), on the back ( Odontomyia garatas ) and on the yellow spot dongae ( Ptecticus aurifer ), preferably on the dongae ( Ptecticus tenebrifer ) or on the American dongae ( Hermetia ) illucens ).
본 발명의 일 실시형태에서 동애등에는 아메리카동에등에(Hermetia illucens)이다.In one embodiment of the present invention, it is an American dongae ( Hermetia illucens ).
본 발명의 일 실시형태에서, 상기 락토코커스 플란타룸 (Lactococcus plantarum) 균주 (기탁번호 KACC 13445) 또는 이의 배양액을 곤충 유충의 사료 조성물로서 제공하는 하는 경우, 용화율 및 우화율에는 영향을 미치지 않으면서도 유충 시기를 단축시킴으로써 성충으로의 빠른 진화를 유도하였다. 이와 같은 빠른 성장은 산란수 및 개체수를 증가시켜 생산성을 증가시키므로 바람직하다. In one embodiment of the present invention, when the Lactococcus plantarum strain (Accession No. KACC 13445) or a culture solution thereof is provided as a feed composition for insect larvae, if the dissolution rate and the evolution rate are not affected It also induced rapid evolution into adults by shortening the larval period. Such rapid growth is preferable because it increases the number of spawning and the number of individuals to increase productivity.
뿐만 아니라, 본 발명의 다른 실시형태에서, 상기 락토코커스 플란타룸 (Lactococcus plantarum) 균주 (기탁번호 KACC 13445) 또는 이의 배양액을 포함하는 사료 조성물은 대조군에 비해 유충의 무게를 증가시킬 수 있다. 유충 자체를 동물의 사료로 사용하기 위해 사육하는 농가에서 이러한 유충의 무게 증가는 경제성을 향상시키므로 바람직하다.In addition, in another embodiment of the present invention, the Lactococcus plantarum strain (Accession No. KACC 13445) or a feed composition comprising a culture solution thereof can increase the weight of the larvae compared to the control. In a farm where the larvae themselves are raised to be used as animal feed, an increase in the weight of these larvae improves economic feasibility, so it is preferable.
본 발명에 의하면, 상기 사료 조성물은 생육을 증진시키기 위한 용도로 제공되는 경우, 유충의 종에 제한이 없으나, 악취를 저감하기 위한 용도로 제공되는 경우, 상기 유충은 바람직하게는 동애등에 유충일 수 있다. According to the present invention, when the feed composition is provided for the purpose of promoting growth, there is no restriction on the species of the larvae, but when provided for the purpose of reducing the odor, the larvae are preferably larvae of Dongae, etc. .
동애등에 유충은 남은 음식물과 같은 유기성폐기물을 먹이로 사용하므로 먹이활동, 즉, 유기물을 분해하는 과정에서 질소 화합물, 예컨대 암모니아와 같은 악취(가스)가 다량 발생될 수 있다. 본 발명에 따른 락토코커스 플란타룸 (Lactococcus plantarum) 균주 (기탁번호 KACC 13445)를 포함하는 사료 조성물은 암모니아와 같은 질소 화합물의 생성을 낮출 수 있으므로 사육장 내 악취를 저감시킬 수 있다. Since the caterpillars use organic waste such as leftover food as food, a large amount of odor (gas) such as nitrogen compounds, such as ammonia, may be generated during feeding activities, that is, decomposing organic matter. The feed composition comprising the Lactococcus plantarum strain (Accession No. KACC 13445) according to the present invention can reduce the production of nitrogen compounds such as ammonia, so it is possible to reduce odors in the breeding grounds.
본 발명에 있어서 상기 사료는 곤충류, 어류, 조류 또는 포유류의 사료 일 수 있으며, 바람직하게, 축산법 제2조 제1호 및 동법 시행규칙 제2조 각호에서 정의하고 있는, 야생습성이 순화되어 사육하기에 적합하며 농가의 소득증대에 기여할 수 있는 곤충 또는 이의 유충, 가축 또는 수산생물의 사료일 수 있다. 상기 가축에는 소, 말, 노새, 당나귀, 염소, 산양, 면양, 사슴, 돼지, 토끼, 가금류 등일 수 있으며, 가금류에는 닭, 칠면조, 오리, 타조, 거위, 메추리 등, 바람직하게는 닭일 수 있으나, 사육하여 축산물을 얻기에 적합한 것이라면 이에 제한되는 것은 아니다. 상기 "축산물"은 축산법 제2조 3호의 정의인, 가축에서 생산된 고기, 젖, 알, 꿀과 이들의 가공품, 원피 (원모피를 포함한다), 원모, 기타 가축의 생산물로서 농림부령이 정하는 것을 의미한다. 또한 반려동물을 포함하여 개, 고양이 등일 수 있으나, 이에 제한되는 것은 아니다.In the present invention, the feed may be a feed for insects, fish, birds or mammals, and preferably, the wild habits defined in
발명의 용어 "사료"는 동물이 먹고, 섭취하며, 소화시키기 위한 또는 이에 적당한 임의의 천연 또는 인공 규정식, 한끼식 등 또는 상기 한끼식의 성분을 의미한다. 일 양태로, 본 발명의 락토코커스 플란트룸 균주를 함유하는 사료용 조성물에는 농후사료, 조사료 및/또는 특수사료가 포함될 수 있다.The term "feed" in the present invention means any natural or artificial diet, meal, etc., or a component of said meal, intended for or suitable for being eaten, consumed, and digested by an animal. In one aspect, the composition for feed containing the Lactococcus plantrum strain of the present invention may include a concentrated feed, roughage and/or special feed.
농후사료에는 밀, 귀리, 옥수수 등의 곡류를 포함하는 종자열매류, 곡물을 정제하고 얻는 부산물로서 쌀겨, 밀기울, 보릿겨 등을 포함하는 겨류, 콩, 유체, 깨, 아마인, 코코야자 등을 채유하고 얻는 부산물인 깻묵류와 고구마, 감자 등에서 녹말을 뺀 나머지인 녹말찌꺼기의 주성분인 잔존녹말질류 등의 찌꺼기류, 어분, 물고기찌꺼기, 어류에서 얻은 신선한 액상물을 농축시킨 것인 피시솔루블, 육분, 혈분, 우모분, 탈지분유, 우유에서 치즈, 탈지유에서 카제인을 제조할 때의 잔액인 훼이(whey)를 건조한 건조훼이 등의 동물질사료, 효모, 클로렐라, 해조류 등이 있다.In the enriched feed, seed fruits containing grains such as wheat, oats, and corn, bran containing rice bran, bran, barley bran, etc. Sesame cake, which is a by-product obtained from cooking, and residues such as residual starchy substances, which are the main components of starch residue, which is the remainder of starch residue from sweet potatoes and potatoes, fish meal, fish residue, and fish soluble, which is a concentrated fresh liquid product obtained from fish , animal feed such as dried whey, yeast, chlorella, seaweed, etc., which are dried whey, which is the remainder of the production of casein from milk, cheese, and casein from skim milk.
조사료에는 야초, 목초, 풋베기 등의 생초(生草)사료, 사료용 순무, 사료용 비트, 순무의 일종인 루터베어거 등의 뿌리채소류, 생초, 풋베기작물, 곡실(穀實)등을 사일로에 채워 놓고 젖산발효시킨 저장사료인 사일리지(silage), 야초, 목초를 베어 건조시킨 건초, 종축용(種畜用) 작물의 짚, 콩과 식물의 나뭇잎 등이 있다. 특수 사료에는 굴껍떼기, 암염 등의 미네랄 사료, 요소나 그 유도체인 디우레이드이소부탄 등의 요소사료, 천연사료 원료만을 배합했을 때 부족하기 쉬운 성분을 보충하거나, 사료의 저장성을 높이기 위해서 배합사료에 미량으로 첨가하는 물질인 사료첨가물 등이 있다.For roughage, raw grass feed such as wild grasses, grasses and green cuttings, turnips for feed, beets for feed, root vegetables such as luther beargers, a type of turnip, raw herbs, green crops, grains, etc. are placed in a silo. There are silage, which is a stored feed fermented with lactic acid after being filled with lactic acid, hay obtained by cutting and drying grass, straw for breeding crops, and leaves of legumes. Special feed includes mineral feed such as oyster shells and rock salt, urea feed such as urea or its derivative diureide isobutane, supplementing ingredients that are easily lacking when only natural feed ingredients are blended, or added to formulated feed to increase feed storability. There are feed additives, which are substances added in trace amounts.
본 발명은 또한, 락토코커스 플란타룸 (Lactococcus plantarum) 균주 (기탁번호 KACC 13445) 또는 이의 배양액을 포함하는 조성물을 동애등에 유충에게 급이하는 단계;를 포함하는 동애등에의 사육에서 악취를 저감시키는 방법을 제공한다. The present invention also provides Lactococcus plantarum ( Lactococcus plantarum ) strain (Accession No. KACC 13445) or the step of feeding the larvae to the larvae of Dongae, etc. with a composition containing a culture solution thereof; provides a method for reducing odor in breeding of Dongae, etc.
본 발명에 의하면, 상기 동애등에의 유충에게 먹이와 함께 락토코커스 플란타룸 (Lactococcus plantarum) 균주 (기탁번호 KACC 13445) 또는 이의 배양액을 포함하는 조성물을 제공하면 균주를 제공하지 않은 군 및/또는 다른 균종, 예컨대 바실러스 서브틸리스 균주를 제공한 군과 비교하여 악취 성분의 발생을 현저하게 저감시켰다. According to the present invention, Lactococcus plantarum ( Lactococcus ) together with food to the larvae of the larvae plantarum ) strain (Accession No. KACC 13445) or a composition containing a culture solution thereof, compared to the group that did not provide the strain and/or other strains, such as the group provided with the Bacillus subtilis strain, the occurrence of odor components was significantly reduced reduced considerably.
본 발명은 또한, 락토코커스 플란타룸 (Lactococcus plantarum) 균주 (기탁번호 KACC 13445) 또는 이의 배양액을 포함하는 조성물을 동애등에 유충에게 급이하는 단계;를 포함하는 동애등에를 이용한 유기성 폐기물 처리에서의 악취저감 방법을 제공한다. The present invention also provides Lactococcus plantarum ( Lactococcus plantarum ) strain (Accession No. KACC 13445) or a step of feeding the larvae to the larvae of the larvae, etc. containing a composition containing a culture medium thereof; provides a method for reducing odor in organic waste treatment using dongae.
본 발명에 의하면, 상기 방법은 암모니아와 같은 악취유발 화합물의 생성량을 저감시키는 것일 수 있다. According to the present invention, the method may be to reduce the amount of the odor-causing compound, such as ammonia.
본 발명에서 용어 '급이'는 대상에게 먹이를 제공하는 것을 의미한다. In the present invention, the term 'feeding' means providing food to a subject.
본 발명에 의하면, 상기 급이는 락토코커스 플란타룸 (Lactococcus plantarum) 균주 (기탁번호 KACC 13445) 또는 이의 배양액을 포함하는 조성물을 유기성 폐기물과 혼합하거나, 유기성 폐기물에 스프레이하는 것일 수 있으나, 이에 제한되는 것은 아니며, 대상에게 제공할 수 있는 방법이면 어느 것이든 가능하다. According to the present invention, the feeding is Lactococcus plantarum ( Lactococcus plantarum ) strain (Accession No. KACC 13445) or a composition comprising a culture solution thereof mixed with organic waste or spraying on organic waste, but limited thereto It is not possible, and any method that can be provided to the target is possible.
본 발명은 또한, 락토코커스 플란타룸 (Lactococcus plantarum) 균주 (기탁번호 KACC 13445) 또는 이의 배양액을 포함하는 조성물을 동애등에 유충에게 급이하는 단계;를 포함하는 동애등에를 이용한 유기성 폐기물 처리 속도의 향상 방법을 제공한다. The present invention also provides Lactococcus plantarum ( Lactococcus plantarum ) strain (Accession No. KACC 13445) or the step of feeding the larvae to the larvae of Dongae, etc. with a composition containing a culture solution thereof; provides a method of improving the rate of organic waste treatment using Dongae, etc.
본 발명에 따른 상기 조성물은 동애등에의 유충 기간을 줄이고, 성장 속도 및 개체수를 빠르게 증가시킬 수 있다. 따라서, 동애등에 개체수 증가를 통해 유기성 폐기물의 처리속도가 향상될 수 있다. The composition according to the present invention can reduce the larval period of Dongae, etc., and rapidly increase the growth rate and number of individuals. Therefore, the processing speed of organic waste can be improved by increasing the number of dongae, etc.
본 발명에 따른 락토코커스 플란타룸 (Lactococcus plantarum) 균주 또는 이의 배양액을 포함하는 곤충 유충 사료 조성물의 식이는 동애등에 유충의 사육시 발생되는 악취를 저감시킬 수 있으므로, 사육장 근무환경 개선, 작업효율 증대 및 농가 주변 민원의 발생을 줄일 수 있어 산업상 유리하다. 뿐만 아니라, 동애등에의 유충 기간을 단축시키고, 생육을 증진시키며, 폐사율을 낮추므로 동애등에의 생산성을 향상시키고, 유기성 폐기물 처리장치의 효율을 증가시킬 수 있다.Lactococcus plantarum according to the present invention ( Lactococcus plantarum ) strain or its culture solution can reduce odors generated during breeding of larvae such as Dongae, etc. is advantageous In addition, it is possible to shorten the larval period of Dongae, etc., improve the growth, and lower the mortality rate, thereby improving the productivity of Dongae, etc., and increasing the efficiency of the organic waste treatment apparatus.
도 1은 락토코커스 플란타룸 (Lactococcus plantarum) 균주 (기탁번호 KACC 13445)를 포함하는 먹이가 제공된 동애등에 유충과 대조군의 동애등에 유충의 생육증진 효과를 확인한 결과이다.
도 2는 동애등에 유충의 사육에서 락토코커스 플란타룸 (Lactococcus plantarum) 균주 (기탁번호 KACC 13445), 바실러스 서브틸리스 (bacillus subtilis) 균주 (기탁번호 KACC 19726)의 제공 여부에 암모니아 발생량 차이를 평가한 도이다. 1 is a Lactococcus plantarum ( Lactococcus plantarum ) strain (Accession No. KACC 13445) is the result of confirming the growth promoting effect of the larvae on the larvae and the control group on the larvae provided with food containing the strain (Accession No. KACC 13445).
2 is a Lactococcus plantarum strain (Accession No. KACC 13445), Bacillus subtilis strain (Accession No. KACC 19726) in the breeding of larvae in Dongae, etc. Evaluating the difference in ammonia generation in whether or not to provide it is one road
동애등에는 남은 음식물과 같은 유기성폐기물의 처리에 이용되고, 그 자체만으로도 고단백 사료로서 유용하기 때문에 동애등에를 효율적이고 환경친화적으로 대량생산하는 다양한 방법의 개발이 요구된다. 본 발명의 발명자들은 동애등에 유충으로부터 유래된 미생물을 활용하여 유충의 생육환경을 개선하여 성장성을 향상시키고, 동애등에 유충 사육 환경에서 발생하는 악취를 저감함으로써 작업환경을 개선할 수 있는 방법을 개발하여 본 발명을 완성하게 되었다. Dongae, etc. is used for the treatment of organic waste such as leftover food and is useful as a high-protein feed by itself. The inventors of the present invention improve the growth environment of larvae by using microorganisms derived from larvae on larvae, etc., and develop a method that can improve the working environment by reducing odors generated in the larvae breeding environment on larvae, etc. The present invention has been completed.
이하, 구체적인 실시예를 통해 본 발명을 보다 상세히 설명한다. 그러나 이들 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로서, 본 발명의 범위가 이들 실시예에 의해 한정되는 것은 아니다.Hereinafter, the present invention will be described in more detail through specific examples. However, these examples are only for explaining the present invention in more detail, and the scope of the present invention is not limited by these examples.
시험예 1. 성장 증진 효과 검증Test Example 1. Verification of growth promotion effect
락토코커스 균주로써 락토코커스 플란타룸 (Lactococcus plantarum) 균주 (기탁번호 KACC 13445)를 분양받아 사용하였다. 동에등에(아메리카동에등에; Hermetia illucens) 유충을 실험군과 대조군으로 나누고, 각 군당 100마리의 유충이 포함되도록 하였다. 실험군에 제공하는 먹이에는 락토코커스 플란타룸 (Lactococcus plantarum) 균주 (기탁번호 KACC 13445)를 제공하고, 대조군에는 증류수를 제공하였다. 일주일간 급이한 후, 유충의 무게를 측정하였으며 이를 하기 도 1에 나타내었다. As a Lactococcus strain, Lactococcus plantarum ( Lactococcus plantarum ) strain (Accession No. KACC 13445) was acquired and used. The larvae of Dongaedungae (American Dongaedeungae; Hermetia illucens ) were divided into an experimental group and a control group, and 100 larvae were included in each group. The food provided to the experimental group included Lactococcus plantarum ( Lactococcus plantarum ) strain (Accession No. KACC 13445) was provided, and distilled water was provided to the control group. After feeding for a week, the weight of the larvae was measured and shown in FIG. 1 below.
도 1에 나타난 바와 같이, 락토코커스 플란타룸 균주가 포함된 먹이를 제공받은 동애등에 유충은 대조군에 비해 체중이 증가하는 것으로 나타났다. As shown in FIG. 1 , it was found that the larvae of Dongae, etc., which were provided with food containing the Lactococcus plantarum strain, increased in weight compared to the control group.
시험예 2. 생존율 평가Test Example 2. Evaluation of survival rate
동에등에 유충을 실험군과 대조군으로 나누고, 각 군당 100마리의 유충이 포함되도록 하였다. 실험군에 제공하는 먹이에는 락토코커스 플란타룸 (Lactococcus plantarum) 균주 (기탁번호 KACC 13445)를 제공하고, 대조군에는 증류수를 제공하였으며, 유충의 생존율을 평가하였다. 실험은 3회 반복하여 평균값을 구하였다. The larvae were divided into an experimental group and a control group, and 100 larvae were included in each group. Lactococcus plantarum strain (Accession No. KACC 13445) was provided to the food provided to the experimental group, and distilled water was provided to the control group, and the survival rate of the larvae was evaluated. The experiment was repeated 3 times to obtain an average value.
표 1에 나타난 바와 같이, 동애등에 유충의 장내에서 유래된 락토코커스 플란타룸 (Lactococcus plantarum) 균주 (기탁번호 KACC 13445)는 동애등에의 생존에 유의적인 영향을 주지 않는 것으로 나타났다. As shown in Table 1, Lactococcus plantarum ( Lactococcus ) derived from the intestine of larvae such as Dongae plantarum ) strain (Accession No. KACC 13445) was found not to have a significant effect on the survival of Dongae, etc.
KACC 13445Lactococcus plantarum
KACC 13445
시험예 3. 암모니아 발생량 평가(ppm)Test Example 3. Ammonia generation amount evaluation (ppm)
동애등에 유충을 3개의 군으로 나누어, 하나의 군에는 동애등에 유충에게 제공하는 먹이에 락토코커스 플란타룸 (Lactococcus plantarum) 균주 (기탁번호 KACC 13445)를 제공하고, 다른 하나의 군에는 바실러스 서브틸리스 (bacillus subtilis) 균주 (기탁번호 KACC 19726)를 제공하고, 나머지 군에는 증류수를 제공한 뒤, 3일 동안 암모니아 발생량을 평가하였으며, 이를 하기 도 2 및 표 2에 나타내었다. Lactococcus plantarum ( Lactococcus plantarum) is divided into three groups, the larvae of the larvae on the larvae of the larvae of the larvae of plantarum ) strain (Accession No. KACC 13445) is provided, the other group is provided with a Bacillus subtilis strain (Accession No. KACC 19726), and distilled water is provided to the remaining group, followed by ammonia for 3 days The amount of generation was evaluated, and it is shown in FIG. 2 and Table 2 below.
(ppm)NH 3 emission
(ppm)
(KACC 13445)Lactococcus plantarum
(KACC 13445)
KACC 19726Bacillus subtilis
KACC 19726
표 2 및 도 2에 나타낸 바와 같이, 락토코커스 플란타룸 (Lactococcus plantarum) 균주 (기탁번호 KACC 13445)를 제공한 군은 대조군에 비해 암모니아 발생량을 현저히 감소시켰으며, 바실러스 서브틸리스 (bacillus subtilis) 균주 (기탁번호 KACC 19726)는 오히려 암모니아 발생량을 증가시켜 악취를 더욱 유발하는 것으로 나타났다. 이러한 작용 효과는 본 발명의 락토코커스 속 균주의 현저한 효과로서, 본 발명에 따른 락토코커스 속 균주, 특히, 락토코커스 플란타룸 (Lactococcus plantarum) 균주 (기탁번호 KACC 13445)가 악취 저감에 우수한 효과가 있음을 보여준다. As shown in Table 2 and Figure 2, the group provided with the Lactococcus plantarum strain (Accession No. KACC 13445) significantly reduced ammonia generation compared to the control group, and Bacillus subtilis ( bacillus subtilis ) It was found that the strain (Accession No. KACC 19726) rather increased the amount of ammonia generated and caused more odor. This effect is a remarkable effect of the Lactococcus sp. strain of the present invention, and the Lactococcus sp. strain according to the present invention, in particular, the Lactococcus plantarum strain (Accession No. KACC 13445) has an excellent effect in reducing odor. show that there is
Claims (11)
상기 조성물은 곤충 유충의 생육을 증진시키고, 유충 기간을 단축시키는 것인, 사료 조성물.According to claim 1,
The composition enhances the growth of insect larvae and shortens the larval period, the feed composition.
상기 곤충 유충은 동애등에 유충인, 사료 조성물.3. The method of claim 2,
The insect larvae are larvae such as Dongae, feed composition.
상기 조성물은 유충의 먹이활동에서 발생되는 악취를 저감시키는 것인, 사료 조성물.3. The method of claim 2,
The composition is to reduce the odor generated from the feeding activity of the larvae, the feed composition.
악취 유발물질은 암모니아인, 사료 조성물.5. The method of claim 4,
The odor-causing substance is ammonia, the feed composition.
상기 조성물은 유충의 먹이활동에서 발생되는 암모니아의 생성량을 저감시키는 것인, 사료 조성물.According to claim 1,
The composition is to reduce the amount of ammonia generated in the feeding activity of the larvae, the feed composition.
상기 방법은 암모니아의 생성량을 저감시키는 것인, 방법.9. The method of claim 8,
The method is to reduce the amount of ammonia produced.
상기 급이는 락토코커스 플란타룸 (Lactococcus plantarum) 균주 (기탁번호 KACC 13445) 또는 이의 배양액을 포함하는 조성물을 유기성 폐기물과 혼합하거나, 유기성 폐기물에 스프레이하는 것인, 방법.9. The method of claim 8,
The feeding is Lactococcus plantarum ( Lactococcus plantarum ) strain (Accession No. KACC 13445) or a composition comprising a culture solution thereof mixed with organic waste, or spraying on organic waste, a method.
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