KR102575766B1 - Feed composition for reducing odor comprising microorganisms of Lactococcus sp. - Google Patents

Abstract

The present invention relates to a composition for reducing odor containing microorganisms of the genus Lactococcus, and preferably, a feed composition for reducing odor containing microorganisms of the genus Lactococcus, which can reduce the odor generated in the breeding environment of black soldier flies, etc. The present invention relates to a composition for reducing odor containing Lactococcus microorganisms for reducing odor in the treatment of organic waste.

Description

Feed composition for reducing odor comprising microorganisms of Lactococcus sp.}

The present invention relates to a composition for reducing odor containing microorganisms of the genus Lactococcus, and preferably, a feed composition for reducing odor containing microorganisms of the genus Lactococcus, which can reduce the odor generated in the breeding environment of black soldier flies, etc. The present invention relates to a composition for reducing odor containing Lactococcus microorganisms for reducing odor in the treatment of organic waste.

Black soldier flies are insects belonging to the family Diptera and are widely distributed in East Asia, including Korea, Japan, China, and Taiwan, and in the United States. More than three generations occur per year, and the first adult insects appear around June and last until around October. They do not make cocoon-shaped pupae, but overwinter as pupae in the form of final instar larvae.

Black soldier flies are representative environmental purification insects that feed only during the larval stage, do not feed during the adult stage, and continue their life cycle in livestock sheds or places where organic waste such as household waste and food waste is stored in the open, making them useful insects. It is being classified. Black soldier fly larvae are omnivorous and are known to generate high temperatures of 50-60°C while feeding on organic waste and secrete antibiotics to inhibit access by other living organisms (Can. J. Microbiol. 6, 115-119). , taking advantage of these characteristics, black soldier flies have recently been used in a clean, effective, and eco-friendly way to treat organic waste such as leftover food and livestock manure.

In addition, the various antibiotics secreted by black soldier fly larvae are attracting attention in the pharmaceutical industry, and black soldier fly larvae are nutritionally excellent, with a protein content of more than 40% and a lipid content of more than 30% of the total. It is used as a high-quality feed for livestock (J Anim Sci 44, 395-400), and its high lipid content suggests its potential as biofuel (Waste Manag. Res. 27, 603-610).

In the natural world, where competition for survival is fierce, it is difficult to smoothly supply eggs and larvae, so interest in raising black soldier fly larvae is increasing, and methods for mass producing black soldier fly larvae have been devised through various methods. Since the growth process of black soldier fly larvae is accompanied by bad odor due to the decomposition of the larvae, a method is required that can reduce the bad smell, improve the growth of black soldier fly, and improve the working environment of the breeding farm.

The problem to be solved by the present invention is to provide a feed composition containing Lactococcus plantarum strain (accession number KACC 13445) or its culture solution.

Another problem to be solved by the present invention is to provide a method of reducing odor in rearing black soldier flies using a composition containing Lactococcus plantarum strain (accession number KACC 13445) or its culture medium.

Another problem to be solved by the present invention is a method for reducing odor in organic waste treatment using black soldier flies, using a composition containing Lactococcus plantarum strain (accession number KACC 13445) or its culture medium, and organic waste The goal is to provide a method of improving waste disposal speed.

In order to solve the above problems, the present invention provides a feed composition containing Lactococcus plantarum strain (accession number KACC 13445) or its culture solution.

According to the present invention, the feed composition can promote the growth of insect larvae and reduce odor that may be generated in a rearing environment.

According to the present invention, the insect larvae are not particularly limited, but are preferably black soldier fly larvae.

According to the present invention, there are restrictions on the black soldier fly, etc., as long as it belongs to the black soldier fly family. For example, black soldier flies ( Ptecticus tenebrifer ), American black soldier fly ( Hermetia illucens ), black soldier fly ( Allognosta) vagans ), Little black soldier fly ( Microchrysa flaviventris ), Black soldier fly ( Craspedometopon frontale ), black soldier fly ( Odontomyia garatas ), yellow spotted black soldier fly ( Ptecticus aurifer ), preferably black soldier fly ( Ptecticus tenebrifer ) or American black soldier fly ( Hermetia) . illucens ).

In one embodiment of the present invention, the black soldier fly is Hermetia illucens .

In one embodiment of the present invention, when the Lactococcus plantarum strain (Accession No. KACC 13445) or its culture medium is provided as a feed composition for insect larvae, if it does not affect the pupation rate and emergence rate By shortening the larval period, rapid evolution to adults was induced. Such rapid growth is desirable because it increases productivity by increasing the number of eggs and population.

In addition, in another embodiment of the present invention, the feed composition containing the Lactococcus plantarum strain (accession number KACC 13445) or its culture medium can increase the weight of larvae compared to the control group. In farms where the larvae themselves are raised to be used as animal feed, this increase in the weight of the larvae is desirable because it improves economic efficiency.

According to the present invention, when the feed composition is provided for the purpose of promoting growth, there is no limitation on the species of larvae, but when the feed composition is provided for the purpose of reducing odor, the larvae may preferably be black soldier fly larvae. .

Since black soldier fly larvae use organic waste such as leftover food as food, large amounts of odors (gases) such as nitrogen compounds, such as ammonia, may be generated during feeding activities, that is, in the process of decomposing organic matter. The feed composition containing the Lactococcus plantarum strain (accession number KACC 13445) according to the present invention can reduce the production of nitrogen compounds such as ammonia and thus reduce odor in the breeding farm.

In the present invention, the feed may be feed for insects, fish, birds or mammals, and is preferably bred with purified wild habits, as defined in Article 2, Paragraph 1 of the Livestock Act and Article 2 of the Enforcement Rules of the same Act. It is suitable for and can be feed for insects or their larvae, livestock, or aquatic life that can contribute to increasing the income of farmers. The livestock may include cattle, horses, mules, donkeys, goats, goats, sheep, deer, pigs, rabbits, poultry, etc., and the poultry may include chickens, turkeys, ducks, ostriches, geese, quails, etc., preferably chickens. It is not limited to this as long as it is suitable for breeding and obtaining livestock products. The above “livestock products” are defined by Article 2, Paragraph 3 of the Livestock Act, as defined by the Ordinance of the Ministry of Agriculture, Food and Rural Affairs as meat, milk, eggs, honey and their processed products, raw hides (including raw fur), raw wool, and other livestock products produced from livestock. means that Additionally, it may be a dog, cat, etc., including companion animals, but is not limited thereto.

The term "feed" of the invention means any natural or artificial diet, meal, etc., or a component of the meal, for or suitable for eating, ingestion, and digestion by animals. In one aspect, the composition for feed containing the Lactococcus plantrum strain of the present invention may include concentrate feed, roughage feed, and/or special feed.

Concentrated feed includes seeds and fruits, including grains such as wheat, oats, and corn; bran, which includes rice bran, bran, and barley bran, which are by-products obtained from refining grains; soybeans; oil, sesame seeds, linseed, and coco oil; Fish soluble, meat powder, which is a concentrated product of fresh liquid obtained from fish meal, fish waste, fish meal, fish waste, etc., such as residual starch, which is the main component of the starch residue that is left after removing the starch from the by-products such as seed jelly, sweet potatoes, potatoes, etc. , animal feeds such as blood meal, feather meal, skim milk powder, dried whey, which is the residue from milk to cheese, and casein from skim milk, yeast, chlorella, seaweed, etc.

Forage includes raw grass feed such as wild grasses, grasses, and green cuttings, turnips for feed, beets for feed, root vegetables such as rutterbearger, a type of turnip, raw grass, green cuttings, and grains, etc., in silos. There are silage, a stored feed that is stuffed and fermented with lactic acid, field grass, hay made by cutting and drying grass, straw from crops for breeding livestock, and leaves from legumes. Special feeds include mineral feeds such as oyster shells and rock salt, urea feeds such as urea and its derivative diureide isobutane, and mixed feeds to supplement ingredients that are likely to be lacking when mixing only natural feed raw materials, or to increase the storability of the feed. There are feed additives, which are substances added in trace amounts.

The present invention also, Lactococcus plantarum ( Lactococcus plantarum ) strain (accession number KACC 13445) or a composition containing its culture medium to black soldier fly larvae; providing a method for reducing odor in the rearing of black soldier fly, including the step.

According to the present invention, the larvae of the black soldier fly are fed with Lactococcus plantarum ( Lactococcus plantarum ) strain (accession number KACC 13445) or a culture medium thereof, the occurrence of odor components is significantly increased compared to the group that did not provide the strain and/or the group that provided other bacterial species, such as Bacillus subtilis strains. significantly reduced.

The present invention also, Lactococcus plantarum ( Lactococcus plantarum ) strain (accession number KACC 13445) or a composition containing its culture medium to black soldier fly larvae; providing a method for reducing odor in organic waste treatment using black soldier fly, including the step of feeding a composition containing the strain (accession number KACC 13445) or its culture medium.

According to the present invention, the method may be to reduce the amount of odor-causing compounds such as ammonia produced.

In the present invention, the term 'feeding' refers to providing food to a subject.

According to the present invention, the feeding may be done by mixing a composition containing Lactococcus plantarum strain (Accession No. KACC 13445) or its culture medium with organic waste or spraying it on organic waste, but is limited thereto. It is not possible, and any method that can provide it to the target is possible.

The present invention also, Lactococcus plantarum ( Lactococcus plantarum ) strain (accession number KACC 13445) or a composition containing its culture medium to black soldier fly larvae; providing a method of improving the speed of organic waste treatment using black soldier fly, which includes the step of feeding a composition containing the strain (accession number KACC 13445) or its culture medium to black soldier fly larvae.

The composition according to the present invention can reduce the larval period of black soldier flies and rapidly increase the growth rate and population. Therefore, the processing speed of organic waste can be improved by increasing the population of black soldier flies.

Lactococcus plantarum according to the present invention ( Lactococcus Plantarum ) strain or its culture medium can reduce the odor generated when rearing larvae of black soldier flies, etc., thereby improving the working environment in the breeding farm, increasing work efficiency, and reducing the occurrence of civil complaints around farms, which can be used in the industrial industry. It is advantageous. In addition, it can shorten the larval period of black soldier flies, improve growth, and lower the mortality rate, thereby improving the productivity of black soldier flies and increasing the efficiency of organic waste treatment devices.

Figure 1 shows Lactococcus plantarum ( Lactococcus plantarum ) strain (accession number KACC 13445). This is the result of confirming the effect of enhancing the growth of black soldier fly larvae provided with food containing the strain (accession number KACC 13445) and the black soldier fly larvae of the control group.
Figure 2 evaluates the difference in ammonia production depending on whether Lactococcus plantarum strain (Accession number KACC 13445) and Bacillus subtilis strain (Accession number KACC 19726) are provided in the rearing of Dongae larvae. It is one degree.

Since black soldier flies are used to dispose of organic waste such as leftover food and are useful as high-protein feed in themselves, the development of various methods to efficiently and environmentally friendly mass production of black soldier flies is required. The inventors of the present invention have developed a method to improve the growth environment of larvae by using microorganisms derived from black soldier fly larvae to improve growth performance and to improve the working environment by reducing the odor generated in the breeding environment of black soldier fly larvae. This invention has been completed.

Hereinafter, the present invention will be described in more detail through specific examples. However, these examples are only for illustrating the present invention in more detail, and the scope of the present invention is not limited by these examples.

Test Example 1. Verification of growth enhancement effect

Lactococcus plantarum ( Lactococcus) as a Lactococcus strain plantarum ) strain (accession number KACC 13445) was purchased and used. Hermetia illucens larvae were divided into experimental and control groups, and each group contained 100 larvae. The food provided to the experimental group was Lactococcus plantarum ( Lactococcus plantarum ) strain (accession number KACC 13445) was provided, and distilled water was provided to the control group. After feeding for a week, the weight of the larvae was measured and is shown in Figure 1 below.

As shown in Figure 1, black soldier fly larvae provided with food containing the Lactococcus plantarum strain showed increased body weight compared to the control group.

Test Example 2. Survival rate evaluation

The larvae were divided into experimental and control groups, and each group contained 100 larvae. Lactococcus plantarum strain (accession number KACC 13445) was provided as food to the experimental group, distilled water was provided to the control group, and the survival rate of larvae was evaluated. The experiment was repeated three times and the average value was obtained.

As shown in Table 1, Lactococcus plantarum ( Lactococcus) derived from the intestines of Dongae larvae plantarum ) strain (accession number KACC 13445) was found to have no significant effect on the survival of black soldier flies.

survival rate #One #2 #3 average control group 99.00 98.00 100.00 99.00 Lactococcus plantarum
KACC 13445 92.00 99.00 99.00 96.67

Test Example 3. Evaluation of ammonia generation (ppm)

The black soldier fly larvae were divided into three groups, and one group contained Lactococcus plantarum ( Lactococcus) in the food provided to the black soldier fly larvae. plantarum ) strain (accession number KACC 13445), one group was provided with a Bacillus subtilis strain (accession number KACC 19726), and the remaining group was provided with distilled water and ammonia for 3 days. The amount generated was evaluated, and it is shown in Figure 2 and Table 2 below.

NH ₃ generation amount
(ppm) Elapsed days (days) 0 One 2 3 control group 0.00 0.00 0.50 7.80 Lactococcus plantarum
(KACC 13445) 0.00 0.00 0.50 1.30 Bacillus subtilis
KACC 19726 0.00 0.00 1.53 27.33

As shown in Table 2 and Figure 2, the group provided with Lactococcus plantarum strain (accession number KACC 13445) significantly reduced ammonia production compared to the control group, and Bacillus subtilis ( bacillus subtilis ) The strain (accession number KACC 19726) was found to actually increase the amount of ammonia produced, causing further odor. This effect is a remarkable effect of the Lactococcus genus strain of the present invention, and the Lactococcus genus strain according to the present invention, especially the Lactococcus plantarum strain (accession number KACC 13445), has an excellent effect in reducing odor. It shows that there is.

Claims (11)

A feed composition for larvae of Dongae, including Lactococcus plantarum strain (accession number KACC 13445) or its culture solution. According to paragraph 1,
The composition is a feed composition for black soldier fly larvae, which promotes the growth of black soldier fly larvae and shortens the larval period. delete According to paragraph 1,
The composition is a feed composition for larvae of black soldier flies, etc., which reduces odor generated during the feeding activities of larvae. According to paragraph 4,
The odor-causing substance is ammonia, a feed composition for black soldier fly larvae. According to paragraph 1,
The composition is a feed composition for larvae of black soldier flies, etc., which reduces the amount of ammonia produced during the feeding activities of larvae. Lactococcus plantarum ( Lactococcus plantarum ) strain (accession number KACC 13445) or a composition containing its culture medium to black soldier fly larvae. A method for reducing odor in the rearing of black soldier fly. Lactococcus plantarum ( Lactococcus plantarum ) strain (accession number KACC 13445) or a composition containing its culture medium to black soldier fly larvae; a method for reducing odor in organic waste treatment using black soldier fly, including. According to clause 8,
The method is to reduce the amount of ammonia produced. According to clause 8,
The feeding is Lactococcus plantarum ( Lactococcus plantarum ) strain (accession number KACC 13445) or a composition containing its culture medium is mixed with organic waste or sprayed on organic waste. Lactococcus plantarum ( Lactococcus plantarum ) strain (accession number KACC 13445) or a composition containing its culture medium to black soldier fly larvae; a method of improving the speed of organic waste treatment using black soldier fly, including the step of feeding a composition containing the strain (accession number KACC 13445) or its culture medium.