KR20210042741A - Composition for feeding of Ptecticus tenebrifer - Google Patents

Composition for feeding of Ptecticus tenebrifer Download PDF

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KR20210042741A
KR20210042741A KR1020190125668A KR20190125668A KR20210042741A KR 20210042741 A KR20210042741 A KR 20210042741A KR 1020190125668 A KR1020190125668 A KR 1020190125668A KR 20190125668 A KR20190125668 A KR 20190125668A KR 20210042741 A KR20210042741 A KR 20210042741A
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동국대학교 경주캠퍼스 산학협력단
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Abstract

The present invention relates to a feed composition for Ptecticus tenebrifer such as food waste, chicken manure, waste cooking oil, and fermented microorganisms. According to the present invention, by including food waste, chicken manure, waste cooking oil, and fermented microorganisms in the feed for Ptecticus tenebrifer, it is possible to effectively improve the growth of Ptecticus tenebrifer at low cost.

Description

동애등에 사료용 조성물 {Composition for feeding of Ptecticus tenebrifer}Composition for feeding of Dongae et al. {Composition for feeding of Ptecticus tenebrifer}

본 발명은 동애등에 사료용 조성물에 관한 것이다.The present invention relates to a composition for feed in Dongae and the like.

건조 동애등에의 번데기는 약 42%의 단백질과 35%의 지방을 함유하고 있으며 오메가 3 지방산이 풍부하여 사료로 가공하여 이용시 닭, 돼지, 무지개송어, 및 메기 등에서 성장 증진 효과가 기대되고 있다. 동애등에는 무지개송어의 사료로 공급하는 경우 어분의 25%까지 대체가 가능하며 동애등에의 지방 및 단백질을 분리하여 60% 이상의 단백질을 함유한 원료 사료로 개발이 가능할 것으로 기대되고 있다. 최근, 캐나다의 엔테라(Enterra)사는 동애등에의 유충을 원료로 한 연어, 송어 등의 양식용 사료를 개발하여 2017년 캐나다식품검사청의 승인을 획득하였다.The pupae of the dried Dongae et al. contain about 42% protein and 35% fat, and are rich in omega 3 fatty acids. When processed into feed, it is expected to increase growth in chickens, pigs, rainbow trout, and catfish. If supplied as feed for rainbow trout to Dongae, it can replace up to 25% of fish meal, and it is expected that it can be developed as a raw material feed containing more than 60% protein by separating fat and protein from Dongae, etc. Recently, Enterra of Canada developed feed for aquaculture such as salmon and trout made from larvae of Dongae et al., and obtained approval from the Canadian Food Inspection Agency in 2017.

앞서 살펴본 바와 같이 많은 연구를 통해 동애등에의 사료 원료로서 이용 가능성이 확인되고 있으나, 경제성 등의 문제로 활용도는 높지 않은 실정이다. 그럼에도 불구하고 동애등에는 해바라기, 팜, 콩 등 식물자원에 비하여 지질 함량이 높을 뿐만 아니라, 토지이용률(사육개체수/단위면적)이 높은 장점이 있기 때문에 저가로 대량의 지질을 생산할 수 있는 새로운 바이오매스로서의 주목받고 있다. 따라서 동애등에의 생산량을 향상시킬 수 있으면서도 가격이 저렴한 사료 개발이 필요한 실정이다. 동애등에의 사료는 종래 음식물 폐기물이 주로 사용되고 있으나, 동애등에의 생육이 충분하지 않아 경제성을 확보하기 어려운 실정이다.As discussed above, the possibility of using it as a feed ingredient to Dongae et al. has been confirmed through many studies, but its utilization is not high due to problems such as economic feasibility. Nevertheless, Dongae et al. have a high lipid content compared to plant resources such as sunflower, palm, and soybeans, as well as a high land use rate (number of breeding individuals/unit area), a new biomass that can produce a large amount of lipids at low cost. Is attracting attention as Therefore, it is necessary to develop a feed that can improve the production of Dongae and the like and yet have an inexpensive price. As for feed to Dongae et al., conventional food waste is mainly used, but it is difficult to secure economic feasibility due to insufficient growth to Dongae et al.

대한민국 공개공보 제10-2018-0027455호(2018.03.14)Republic of Korea Public Publication No. 10-2018-0027455 (2018.03.14)

일 구체예에 따르면, 생산비용이 낮고 동애등에 유충의 생산량을 향상시킬 수 있는 사료 조성물을 제공한다.According to one embodiment, the production cost is low and provides a feed composition capable of improving the production of larvae in Dongae and the like.

일 양상은, 음식물 폐기물, 축분, 폐식용유, 및 발효 미생물을 포함하는 동애등에 사료용 조성물을 제공한다. One aspect provides a feed composition for food waste, animal feed, waste cooking oil, and Dongae including fermenting microorganisms.

동애등에(Ptecticus tenebrifer)는 파리목 동애등에과에 속하는 곤충으로서 크기는 13 내지 20mm이고 한국, 일본, 중국, 타이완 등에 분포하며, 재래식 화장실 부근에서 흔히 관찰되는 곤충이다. 상기 조성물은 동애등에 유충의 사료일 수 있으며, 부화 후 4일 이후, 5일 이후, 6일 이후, 또는 7일 이후의 유충의 사료로서 적합한 것일 수 있다. Ptecticus tenebrifer ( Ptecticus tenebrifer ) is an insect belonging to the family Flies, the size of 13 to 20mm, distributed in Korea, Japan, China, and Taiwan, and is an insect commonly observed near conventional toilets. The composition may be feed for larvae in Dongae, etc., and may be suitable as feed for larvae after 4 days, 5 days, 6 days, or 7 days after hatching.

음식물 폐기물은 가정 또는 식당에서 배출되는 음식물 쓰레기를 의미하며, 분뇨 및 폐식용유는 포함하지 않는 것을 의미한다. 본 명세서에서 음식물 폐기물은 음식물, 분뇨 등을 모두 포함하는 개념인 유기성 폐기물과 구별되는 것으로서, 본 발명자는 유기성 폐기물로서 음식물 폐기물, 축분, 및 폐식용유의 비율을 규명하고, 발효 미생물을 혼합하여 제조한 사료를 이용하면 저렴한 가격으로 동애등에의 성장을 촉진할 수 있음을 확인하였다. 상기 음식물 폐기물의 주성분은 건조 전에 평균적으로 수분 80%, 조단백 5%, 조지방 3%, 조섬유 1%, 가용무질소물 9%, 조회분 2%이며(남은 음식물사료 생산이용 제6회 심포지엄 참조), 에너지는 1067kcal로 알려져 있으나 이에 한정되는 것은 아니다. 음식물쓰레기의 성분비는 소규모로 조사하는 경우 지역별, 시기별로 다소 차이가 있을 수 있으나 시설에서 대규모로 수거하여 처리하면 성분의 비율을 일정하게 유지할 수 있다. 상기 음식물 폐기물은 한국에서 입수한 것을 사용할 수 있다. 상기 음식물 폐기물은 살균, 압축, 탈수, 및 건조 중 하나 이상의 공정으로 처리한 것일 수 있다. 상기 살균은 열처리에 의해 살균한 것일 수 있다. 상기 음식물 폐기물은 건조시킨 것을 사용할 수 있으며 수분 함량을 60% 이하, 50% 이하, 40% 이하, 30% 이하, 또는 25% 이하일 수 있으며, 영양소 파괴 또는 부패를 방지하기 위해 20% 이하, 또는 15% 이하로 건조시킨 것일 수 있다. 상기 음식물 폐기물은 수분 함량을 감소시키기 위해 대두피 등의 부형제를 첨가한 것일 수 있다. 상기 음식물 폐기물은 염분 함량이 5% 이하인 것일 수 있다. Food waste refers to food waste discharged from homes or restaurants, and does not include manure and waste cooking oil. In the present specification, food waste is distinguished from organic waste, which is a concept including all food waste, manure, etc., and the inventors identified the ratio of food waste, animal feed, and waste cooking oil as organic waste, and prepared by mixing fermented microorganisms. It was confirmed that the use of feed can promote the growth of Dongae et al. at an affordable price. The main constituents of the food waste are, on average, 80% moisture, 5% crude protein, 3% crude fat, 1% crude fiber, 9% soluble nitrogen-free, and 2% ash content before drying (refer to the 6th Symposium on Production and Use of Remaining Food and Food). , The energy is known to be 1067 kcal, but is not limited thereto. The composition ratio of food waste may vary slightly by region and period when surveyed on a small scale, but the ratio of ingredients can be kept constant by collecting and disposing of it on a large scale at the facility. The food waste may be obtained from Korea. The food waste may be treated by one or more of sterilization, compression, dehydration, and drying. The sterilization may be sterilized by heat treatment. The food waste may be dried and may have a moisture content of 60% or less, 50% or less, 40% or less, 30% or less, or 25% or less, and 20% or less, or 15 to prevent nutrient destruction or spoilage. It may be dried to less than %. The food waste may be obtained by adding an excipient such as soybean husk to reduce the moisture content. The food waste may have a salt content of 5% or less.

상기 축분은 가축의 축사에서 발생하는 배설물로서, 예를 들면 우분, 돈분, 및 계분 중 하나 이상을 포함할 수 있고, 바람직하게는 계분일 수 있다. 본 발명자는 우분, 돈분을 사용하는 경우보다 계분을 사용하는 경우 동애등에의 지방 함량을 증가시킬 수 있음을 확인하였다. 상기 축분, 또는 계분은 시판되는 것을 사용할 수 있다. 상기 축분은 건조된 것일 수 있다. The livestock manure is excrement generated in livestock housing, and may include, for example, one or more of cattle manure, pig manure, and chicken manure, and preferably may be chicken manure. The present inventors have confirmed that it is possible to increase the fat content in Dongae, etc. when using chicken meal than when using cattle and pig meal. The animal feed or chicken meal may be commercially available. The condensate may be dried.

상기 폐식용유는 일반적으로 사용되는 식용 유지류이며, 예를 들면 볶음, 튀김 요리에 사용된 식용유일 수 있으나 특별히 한정되는 것은 아니다.The waste edible oil is generally used edible oils and fats, and may be, for example, edible oil used for stir-fry or frying, but is not particularly limited.

상기 발효 미생물은 오폐수 분해, 수질 개선, 음식물 분해, 분뇨 분해, 퇴비 제조 등에 사용되는 미생물일 수 있다. 상기 발효 미생물은 병원성 세균이 아닌 것으로서, 효모, 락토바실러스속 균주, 및 광합성 세균 중 하나 이상을 포함할 수 있으며, 락토바실러스 균주는 구체적으로 락토바실러스 카세이(Lactobacillus casei)일 수 있다. 상기 발효 미생물은 시중에서 오폐수, 음식물, 또는 분뇨 분해, 퇴비 제조용으로 판매하는 것을 구입하여 사용할 수 있다.The fermenting microorganism may be a microorganism used for wastewater decomposition, water quality improvement, food decomposition, manure decomposition, compost manufacturing, and the like. The fermentation microorganism is not pathogenic bacteria, and may include one or more of yeast, Lactobacillus strain, and photosynthetic bacteria, and the Lactobacillus strain may be specifically Lactobacillus casei . The fermenting microorganisms may be purchased and used for commercially available wastewater, food, or manure decomposition and compost production.

상기 조성물에서 음식물 폐기물의 함량은 50 내지 70 중량%, 또는 55 내지 65 중량%일 수 있고, 상기 축분의 함량은 30 내지 50 중량%, 35 내지 45 중량%, 또는 37 내지 43 중량%일 수 있고, 상기 폐식용유의 함량은 1 내지 3 중량%, 1.5 내지 2.5 중량%, 1.8 내지 2.2 중량%일 수 있고, 상기 발효미생물의 함량은 0.6 내지 1 중량%, 0.7 내지 0.9 중량%일 수 있으며, 바람직하게는 상기 조성물의 조성비는 음식물 폐기물 60 중량%, 축분 40 중량%, 폐식용유 2.0 중량%, 및 발효미생물 0.8중량%일 수 있다. 본 발명자는 상기 조성비에 의할 때 음식물 쓰레기만을 사료로 사용하는 경우보다 마리당 증체량이 191.9% 향상됨을 확인하였다. In the composition, the content of food waste may be 50 to 70% by weight, or 55 to 65% by weight, and the content of the condensate may be 30 to 50% by weight, 35 to 45% by weight, or 37 to 43% by weight, and , The content of the waste cooking oil may be 1 to 3% by weight, 1.5 to 2.5% by weight, 1.8 to 2.2% by weight, and the content of the fermentation microorganism may be 0.6 to 1% by weight, 0.7 to 0.9% by weight, preferably Preferably, the composition ratio of the composition may be 60% by weight of food waste, 40% by weight of animal feed, 2.0% by weight of waste cooking oil, and 0.8% by weight of fermented microorganisms. The present inventors confirmed that the weight gain per head was improved by 191.9% compared to the case of using only food waste as feed according to the above composition ratio.

일 구체예에 따른 동애등에 사료용 조성물은 낮은 가격으로 생산할 수 있으며, 동애등에의 생육을 효과적으로 증가시킬 수 있다. The composition for feed in Dongae and the like according to one embodiment can be produced at a low price, and can effectively increase the growth of Dongae and the like.

도 1은 음식물 폐기물 및 계분의 혼합 비율을 달리하여 동애등에 유충의 건조무게(A), 동애등에의 생존율(B), 및 동애등에의 마리당 증체량(C)을 확인한 결과를 나타낸 것이다.
도 2는 음식물 폐기물 및 폐식용유의 혼합 비율을 달리하여 동애등에 유충의 건조무게(A), 동애등에의 생존율(B), 및 동애등에의 마리당 증체량(C)을 확인한 결과를 나타낸 것이다.
도 3은 음식물 폐기물 및 발효미생물의 혼합 비율을 달리하여 동애등에 유충의 건조무게(A), 동애등에의 생존율(B), 및 동애등에의 마리당 증체량(C)을 확인한 결과를 나타낸 것이다.
도 4 내지 도 6은 음식물 폐기물, 계분, 폐식용유, 및 발효미생물의 혼합 비율을 달리하여 동애등에 유충의 건조무게(A), 동애등에의 생존율(B), 및 동애등에의 마리당 증체량(C)을 확인한 결과를 나타낸 것이다.1 shows the results of confirming the dry weight of larvae in Dongae et al. (A), the survival rate to Dongae et al. (B), and the weight gain per head to Dongae et al. (C) by varying the mixing ratio of food waste and chicken meal.
Figure 2 shows the results of confirming the dry weight (A) of larvae to Dongae, the survival rate to Dongae (B), and the weight gain per head to Dongae (C) by varying the mixing ratio of food waste and waste cooking oil.
Figure 3 shows the results of confirming the dry weight of the larvae to Dongae (A), the survival rate to Dongae (B), and the weight gain per head to Dongae (C) by varying the mixing ratio of food wastes and fermented microorganisms.
4 to 6 show the dry weight (A) of larvae to Dongae, the survival rate to Dongae (B), and the weight gain per head to Dongae (C) by varying the mixing ratio of food waste, chicken meal, waste cooking oil, and fermented microorganisms. It shows the result of checking.

이하 하나 이상의 구체예를 실시예를 통해 보다 상세하게 설명한다. 그러나, 이들 실시예는 하나 이상의 구체예를 예시적으로 설명하기 위한 것으로 본 발명의 범위가 이들 실시예에 한정되는 것은 아니다. Hereinafter, one or more specific examples will be described in more detail through examples. However, these examples are for illustrative purposes only and the scope of the present invention is not limited to these examples.

실시예 1: 음식물 폐기물 및 계분의 최적의 혼합 비율 확인Example 1: Confirmation of the optimal mixing ratio of food waste and chicken meal

동애등에의 생체 내 지질함량을 향상시킬 수 있는 저가 먹이원을 선발하기 위하여 건조 축분(계분), 건조 음식물 폐기물, 발효미생물(Effective Microorganism, EM) 및 폐식용유를 단독 또는 혼합하여 먹이로 공급하였다. 음식물 폐기물은 처리업체(㈜흰돌)로부터 입수하였고 수분 함량은 12.4%로 확인되었다. 건조 계분은 시판되고 있는 제품(올리버)을 사용하였다. 폐식용유는 경주시 소재 음식점에서 튀김에 사용된 폐식용유를 입수해 사용하였다. 발효미생물은 시판되고 있는 EM 활성액(㈜에버미라클)을 구입하여 사용하였다. In order to select a low-cost food source that can improve the in vivo lipid content of Dongae et al., dry livestock meal (chicken meal), dry food waste, fermented microorganisms (Effective Microorganism, EM), and waste cooking oil were supplied as food alone or as a mixture. Food waste was obtained from a treatment company (Hundol Co., Ltd.), and the moisture content was confirmed to be 12.4%. A commercially available product (Oliver) was used as the dry chicken meal. Waste cooking oil was obtained from a restaurant in Gyeongju and used for frying. For fermentation microorganisms, commercially available EM active liquid (Ever Miracle Co., Ltd.) was purchased and used.

이하에서 %는 특별히 다르게 정의하지 않는 한 중량을 기준으로 한 것이다. Hereinafter,% is based on weight unless otherwise defined.

먼저 음식물 폐기물 및 건조 계분을 이용하여 사료를 제작하였다. 구체적으로 사료 총 중량 2 00g을 기준으로 음식물 폐기물과 계분의 중량비가 100:0, 80:20, 60:40, 40:60, 20:80, 0:100가 되도록 6종류의 단독 또는 혼합 사료를 제작하였다. 각 사료 당 부화 후 6일이 경과한 동애등에 유충 약 100두(0.3mg/유충)를 투입하고 11일 후에 생육조사를 실시하였다. 상기 0.3mg/유충은 마리 당 무게의 추정치이며, 동애등에 대량투입시 마리수를 결정하기 위해 사용되는 기준으로서 통상기술자에게 알려져 있다. First, feed was prepared using food waste and dry chicken meal. Specifically, 6 types of single or mixed feed are prepared so that the weight ratio of food waste and chicken meal is 100:0, 80:20, 60:40, 40:60, 20:80, 0:100 based on the total weight of feed 2 00g. Was produced. About 100 heads of larvae (0.3mg/larva) were added to Dongae, etc. 6 days after hatching for each feed, and growth investigation was conducted 11 days later. The 0.3mg/larva is an estimate of the weight per head, and is known to a conventional engineer as a standard used to determine the number of heads when mass-introduced to Dongae.

동애등에 유충의 건조무게를 분석한 결과, 음식물 폐기물과 계분의 혼합비율이 80:20, 60:40, 40:60인 실험군에서는 음식물 폐기물 단독 처리군(100:0)과 비교할 때 계분의 함량 증가에 따라 동애등에 유충의 무게가 증가하였으나, 계분을 80 중량% 이상 함유한 실험군에서는 유충의 무게가 감소하고 계분을 100% 함유한 실험군에서는 오히려 음식물 폐기물 단독처리에 비하여 유충의 무게가 감소하는 것을 확인하였다(도 1의 A 참조). As a result of analyzing the dry weight of larvae in Dongae et al., in the experimental group where the mixing ratio of food waste and poultry was 80:20, 60:40, 40:60, the content of chicken meal increased compared to the food waste treatment group (100:0). As a result, the weight of the larvae increased in Dongae, etc., but the weight of the larvae decreased in the experimental group containing more than 80% by weight of chicken meal, and the weight of the larvae decreased compared to the single treatment of food waste in the experimental group containing 100% chicken meal. (See Fig. 1A).

유충의 생존율을 분석한 결과, 계분을 20 내지 80 중량% 함유한 사료를 공급한 실험군에서는 음식물 폐기물 단독 처리군과 비교할 때 통계적 유의성이 인정되지 않았으나, 계분 100% 실험군에서는 음식물 폐기물 단독 처리군보다 현저히 생존율이 감소하였다(도 1의 B 참조).As a result of analyzing the survival rate of larvae, statistical significance was not recognized in the experimental group fed with feed containing 20 to 80% by weight of fowl meal compared to the food waste alone treatment group, but the 100% chicken meal experimental group was significantly more pronounced than the food waste alone treatment group. The survival rate was decreased (see FIG. 1B).

동애등에 전체 증체량(건조무게/마리수)을 분석한 결과, 계분을 60% 함유한 사료를 공급한 실험군이 가장 증체량이 높게 나타났으며, 마리당 증체량은 계분 40% 함유 사료를 공급한 실험군이 가장 높은 것(183.7%)으로 확인되었으며, 계분의 함유량이 60% 이상인 사료를 처리한 실험군에서는 감소하는 것으로 확인되었다(도 1의 C 참조). As a result of analyzing the total weight gain (dry weight/number of animals) in Dongae, etc., the experimental group that supplied the feed containing 60% chicken meal showed the highest weight gain, and the weight gain per head was the most in the experimental group fed the feed containing 40% chicken meal. It was found to be high (183.7%), and it was found to decrease in the experimental group treated with feed with 60% or more of chicken meal (see C in FIG. 1).

실험 결과를 종합하면, 건조무게가 가장 높은 계분첨가 60% 실험군이 사료로서의 가치가 가장 높은 것으로 확인되었다. In summary, the experimental group with the highest dry weight added 60% chicken manure was found to have the highest value as feed.

실시예 2: 음식물 폐기물 및 폐식용유의 최적의 혼합 비율 확인Example 2: Confirmation of the optimal mixing ratio of food waste and waste cooking oil

총 중량 200g을 기준으로 음식물 폐기물 및 폐식용유를 혼합한 사료를 제작하였다. 폐식용유의 혼합비율은 각각 0, 1, 2, 3, 4, 및 5 중량%로 하였다. 각 사료 당 부화 후 6일이 경과한 동애등에 유충(0.3mg/유충) 약 100두를 투입하고 11일 후에 생육조사를 실시하였다. Based on a total weight of 200 g, a feed was prepared in which food waste and waste cooking oil were mixed. The mixing ratio of the waste cooking oil was 0, 1, 2, 3, 4, and 5% by weight, respectively. About 100 heads of larvae (0.3mg/larva) were added to Dongae etc. 6 days after hatching for each feed, and growth investigation was conducted 11 days later.

동애등에 유충의 건조무게를 확인한 결과, 폐식용유 1, 2, 및 3 중량%를 함유한 사료를 공급한 실험군에서는 음식물 폐기물만을 공급한 실험군보다 오히려 건조무게가 감소하였으며, 폐식용유 4, 5 중량%를 함유한 사료를 공급한 실험군에서는 건조무게가 증가하였다(도 2의 A 참조). As a result of checking the dry weight of the larvae in Dongae et al., the dry weight decreased in the experimental group that supplied feed containing 1, 2, and 3% by weight of waste cooking oil, rather than the experimental group that supplied only food waste, and 4, 5% by weight of waste cooking oil. The dry weight was increased in the experimental group fed with the feed containing (see Fig. 2A).

유충의 생존율을 분석한 결과, 폐식용유를 함유한 모든 실험군은 음식물 폐기물 단독 처리군보다 생존율이 감소하였다(도 2의 B 참조). As a result of analyzing the survival rate of the larvae, the survival rate of all experimental groups containing waste cooking oil decreased compared to the food waste treatment group alone (see B of FIG. 2).

유충의 증체량(건조무게/마리수)을 분석한 결과, 폐식용유 5%를 함유한 사료를 공급한 실험군이 음식물 폐기물 단독 처리군보다 높은 증체량을 나타내었다(도 2의 C 참조). As a result of analyzing the weight gain (dry weight/number of animals) of the larva, the experimental group fed the feed containing 5% of waste cooking oil showed a higher weight gain than the food waste alone treatment group (see FIG. 2C).

실험결과 폐식용유를 함유한 경우 생존율이 다소 감소하더라도 개체의 무게가 증가하여 건조무게 및 증체량이 증가함을 확인하였다. As a result of the experiment, it was confirmed that even if the survival rate decreased slightly when the waste cooking oil was contained, the weight of the individual increased, resulting in an increase in dry weight and weight gain.

실시예 3: 음식물 폐기물 및 발효미생물의 최적의 혼합 비율 확인Example 3: Confirmation of the optimal mixing ratio of food waste and fermentation microorganisms

음식물 폐기물 및 발효미생물을 혼합한 사료를 제조하고 동애등에 생육에 미치는 영향을 확인하였다. 사료 총 중량 200g을 기초로 발효미생물을 0, 0.4, 0.6, 1.2, 1.6, 및 2.0 중량% 함유한 사료를 제작하고, 각 사료 당 부화 후 6일이 경과한 동애등에 유충(0.3mg/유충) 약 100두를 투입하고 11일 후에 생육조사를 실시하였다.A feed mixed with food waste and fermented microorganisms was prepared and the effect on the growth of Dongae et al. was confirmed. A feed containing 0, 0.4, 0.6, 1.2, 1.6, and 2.0% by weight of fermented microorganisms was prepared based on the total weight of the feed 200g, and larvae (0.3mg/larva) on Dongae etc. 6 days after hatching for each feed About 100 heads were added and growth was investigated 11 days later.

동애등에 유충의 건조무게를 확인한 결과, 발효 미생물을 1.2 및 2.0 중량% 함유한 사료를 처리한 실험군은 음식물 폐기물 단독 처리구보다 건조 중량이 증가하였으나, 다른 실험군에서는 통계적으로 유의하게 건조 중량 효과가 나타나지 않았다(도 3의 A 참조). As a result of checking the dry weight of larvae in Dongae et al., the experimental group treated with the feed containing 1.2 and 2.0 wt% fermented microorganisms increased the dry weight compared to the food waste treatment alone, but the other experimental groups did not show a statistically significant dry weight effect. (See Fig. 3A).

유충의 생존율을 분석한 결과, 발효 미생물을 2.0% 함유한 사료를 공급한 실험군을 제외한 모든 실험군에서는 음식물 폐기물 단독 처리군보다 생존율이 감소하였다(도 3의 B 참조). As a result of analyzing the survival rate of the larvae, the survival rate was decreased in all the experimental groups except for the experimental group that supplied feed containing 2.0% of fermented microorganisms compared to the food waste alone treatment group (see B of FIG. 3).

유충의 증체량을 분석한 결과, 발효미생물을 1.2% 함유한 사료를 공급한 실험군에서 가장 높은 증체량을 나타내었다(도 3의 C 참조). As a result of analyzing the weight gain of the larvae, the highest weight gain was shown in the experimental group fed with a feed containing 1.2% fermented microorganisms (see C in FIG. 3).

실시예 4: 음식물 폐기물, 계분, 폐식용유, 및 발효미생물의 최적의 혼합 비율 확인Example 4: Confirmation of the optimal mixing ratio of food waste, chicken meal, waste cooking oil, and fermentation microorganisms

음식물 폐기물, 계분, 폐식용유, 및 발효미생물을 다양한 비율로 혼합하여 사료를 제작하고 동애등에의 생육을 조사하였다. Food waste, poultry, waste cooking oil, and fermented microorganisms were mixed in various ratios to prepare feed, and the growth of Dongae et al. was investigated.

4-1. 계분, 폐식용유, 발효미생물의 함량을 증가시키면서 생육에 미치는 영향 확인4-1. Checking the effect on growth while increasing the content of chicken meal, waste cooking oil, and fermented microorganisms

도 4의 A를 참조하면, 0% 음식물 폐기물, 100% 계분, 5% 폐식용유, 및 2.0% 발효미생물을 혼합한 사료를 공급한 실험군을 제외한 모든 실험군에서 음식물 폐기물 단독 처리군보다 건조 중량이 높은 것으로 확인되었다. 이 중에서 음식물 폐기물 60%, 계분 40%, 폐식용유 2.0%, 발효미생물 0.8%를 혼합한 사료를 공급한 실험군에서 생육이 가장 우수함을 확인하였다. Referring to FIG. 4A, dry weight is higher than that of the food waste alone treatment group in all experimental groups except for the experimental group that supplied feed mixed with 0% food waste, 100% chicken meal, 5% waste cooking oil, and 2.0% fermented microorganisms. Was confirmed. Among them, it was confirmed that the growth was the best in the experimental group fed with a feed mixture of 60% food waste, 40% chicken meal, 2.0% waste cooking oil, and 0.8% fermented microorganisms.

도 4의 B를 참조하면, 0% 음식물 폐기물, 100% 계분, 5.0% 폐식용류, 및 2.0% 발효미생물을 혼합한 사료를 공급한 실험군을 제외하고, 나머지 모든 실험군에서는 음식물 폐기물 단독 처리구에 비하여 생존율에서 통계적으로 유의미한 차이가 나타내지 않았다. Referring to FIG. 4B, except for the experimental group that supplied feed mixed with 0% food waste, 100% chicken meal, 5.0% waste edibles, and 2.0% fermented microorganisms, the remaining experimental groups were compared with the food waste alone treatment group. There was no statistically significant difference in.

도 4의 C를 참조하면, 동애등에의 증체량은 60% 음식물 폐기물, 40% 계분, 2.0% 폐식용류, 및 0.8% 발효미생물을 혼합한 사료를 공급한 실험군이 음식물 폐기물 단독 처리군을 기준으로 191.9%의 증체량을 나타내어 가장 높은 것으로 확인되었다. Referring to FIG. 4C, the weight gain to Dongae et al. was 191.9 based on the food waste alone treatment group in the experimental group that supplied feed mixed with 60% food waste, 40% chicken meal, 2.0% waste edibles, and 0.8% fermented microorganisms. It was found to be the highest by showing% of body weight gain.

4-2. 계분, 발효미생물의 함량을 증가시키고 폐식용유의 함량을 감소시키면서 생육에 미치는 영향 확인4-2. Increase the content of poultry and fermentation microorganisms and check the effect on growth while decreasing the content of waste cooking oil

도 5의 A를 참조하면, 0% 음식물 폐기물, 100% 계분, 1.0% 폐식용류, 및 2.0% 발효미생물을 혼합한 사료를 공급한 실험군을 제외한 모든 처리구에서 음식물 폐기물 단독 처리구보다 높은 생육성적을 나타내었다. 그 중 40% 음식물 폐기물, 60% 계분, 3.0% 폐식용류, 및 1.2% 발효미생물을 혼합한 실험군이 가장 높은 생육성적을 나타내었다. Referring to FIG. 5A, all treatment groups except for the experimental group that supplied feed mixed with 0% food waste, 100% chicken meal, 1.0% waste edibles, and 2.0% fermented microorganisms showed higher viability than the food waste alone treatment group. I got it. Among them, the experimental group in which 40% food waste, 60% chicken meal, 3.0% waste edibles, and 1.2% fermented microorganisms were mixed showed the highest viability.

도 5의 B를 참조하면, 0% 음식물 폐기물, 100% 계분, 5.0% 폐식용류, 및 2.0% 발효미생물을 혼합한 사료를 공급한 실험군 및 80% 음식물 폐기물, 20% 계분, 5.0% 폐식용류, 및 4.0% 발효미생물을 혼합한 사료를 공급한 실험군을 제외한 모든 실험군은 음식물 폐기물 단독 처리구에 비하여 통계적으로 생존율에 유의미한 차이가 없었다. Referring to FIG. 5B, the experimental group and 80% food waste, 20% chicken meal, 5.0% waste edibles, and the experimental group fed with a feed mixed with 0% food waste, 100% chicken meal, 5.0% waste edibles, and 2.0% fermented microorganisms, And there was no statistically significant difference in survival rate in all experimental groups except for the experimental group fed with feed mixed with 4.0% fermented microorganisms compared to the food waste alone treatment group.

도 5의 C를 참조하면, 마리당 증체량은 40% 음식물 폐기물, 60% 계분, 3.0% 폐식용류, 및 1.2% 발효미생물을 혼합한 사료를 공급한 실험군이 음식물쓰레가 단독 처리군보다 높은 169.2%의 증체량을 나타내었다. Referring to FIG. 5C, the weight gain per head was 169.2%, which was higher in the experimental group fed with a mixture of 40% food waste, 60% chicken meal, 3.0% waste edibles, and 1.2% fermented microorganisms. It showed the weight gain.

4-3. 계분의 함량을 증가시키고 폐식용유 및 발효미생물의 함량을 감소시키면서 생육에 미치는 영향 확인4-3. Increasing the content of chicken meal and reducing the content of waste cooking oil and fermentation microorganisms, confirming the effect on growth

도 6의 A를 참조하면, 0% 음식물 폐기물, 100% 계분, 1.0% 폐식용류, 및 0.4% 발효미생물을 혼합한 사료를 공급한 실험군을 제외한 모든 실험군에서 음식물 폐기물 단독 처리군보다 높은 생육성적을 나타내었으며, 그 중 40% 음식물 폐기물, 60% 계분, 3.0% 폐식용류, 및 1.2% 발효미생물을 혼합한 사료를 공급한 실험군이 가장 높은 생육성적을 나타내었다. Referring to FIG. 6A, all experimental groups except for the experimental group that supplied feed containing 0% food waste, 100% chicken meal, 1.0% waste edibles, and 0.4% fermented microorganisms were higher than the food waste alone treatment group. Among them, the experimental group fed with a mixture of 40% food waste, 60% chicken meal, 3.0% waste edibles, and 1.2% fermented microorganisms showed the highest viability.

도 6의 B를 참조하면, 0% 음식물 폐기물, 100% 계분, 1.0% 폐식용류, 및 0.4% 발효미생물을 혼합한 사료를 공급한 실험군을 제외한 모든 실험군은 음식물 폐기물 단독 처리군에 비하여 통계적으로 생존력에서 유의미한 차이가 나타내지 않았다. Referring to FIG.6B, all experimental groups except for the experimental group that supplied feed containing 0% food waste, 100% chicken meal, 1.0% waste edibles, and 0.4% fermented microorganisms were statistically viable compared to the food waste alone treatment group. There was no significant difference in.

도 6의 C를 참조하면, 마리당 증체량은 20% 음식물 폐기물, 80% 계분, 2.0% 폐식용류, 및 0.5% 발효미생물을 혼합한 사료를 공급한 실험군이 음식물쓰레가 단독 처리구보다 높은 166.1%의 증체량을 나타내었다. Referring to FIG. 6C, the weight gain per head was 166.1%, which was higher in the experimental group fed with a mixture of 20% food waste, 80% chicken meal, 2.0% waste edibles, and 0.5% fermented microorganisms. Shown.

상기 실험 결과를 기초로 동애등에의 건조무게, 생존률, 및 증체량을 종합적으로 고려할 때, 음식물 폐기물 60 중량%, 계분 40 중량%, 폐식용유 2 중량%, 및 발효미생물 0.8 중량%를 포함하는 사료를 공급한 실험군이 가장 사료로서 효과가 뛰어난 것으로 확인되었다. When considering the dry weight, survival rate, and weight gain to Dongae et al. based on the above experimental results, feed containing 60% by weight of food waste, 40% by weight of chicken meal, 2% by weight of waste cooking oil, and 0.8% by weight of fermented microorganisms It was confirmed that the supplied experimental group was the most effective as feed.

Claims (6)

음식물 폐기물, 축분, 폐식용유, 및 발효 미생물을 포함하는 동애등에 사료용 조성물.Food waste, animal feed, waste cooking oil, and feed composition including fermented microorganisms, such as Dongae. 제 1 항에 있어서,
상기 음식물 폐기물은 50 내지 70 중량%, 상기 축분은 35 내지 45 중량%, 상기 폐식용유는 1 내지 3 중량%, 상기 발효미생물은 0.6 내지 1 중량%를 포함하는,
동애등에 사료용 조성물.The method of claim 1,
The food waste contains 50 to 70% by weight, the livestock meal is 35 to 45% by weight, the waste edible oil is 1 to 3% by weight, the fermentation microorganisms contain 0.6 to 1% by weight,
Composition for feed in Dongae, etc. 제 1 항에 있어서,
상기 음식물 폐기물은 수분 함량이 25% 이하인,
동애등에 사료용 조성물. The method of claim 1,
The food waste has a moisture content of 25% or less,
Composition for feed in Dongae, etc. 제 1 항에 있어서,
상기 축분은 계분을 포함하는 것인,
동애등에 사료용 조성물.The method of claim 1,
The constituents include poultry,
Composition for feed in Dongae, etc. 제 1 항에 있어서,
상기 발효 미생물은 락토바실러스 카세이를 포함하는 것인,
동애등에 사료용 조성물.The method of claim 1,
Wherein the fermentation microorganism comprises Lactobacillus casei,
Composition for feed in Dongae, etc. 제 1 항에 있어서,
상기 동애등에는 유충인 것인,
동애등에 사료용 조성물.

The method of claim 1,
The larvae in the Dongae, etc.,
Composition for feed in Dongae, etc.
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