KR20220020292A - Method for Storing Fruit Vegetable Scion and Root Stock Seedlings - Google Patents

Abstract

The present invention relates to a storage method of fruit and vegetable scion and rootstock which suppresses excessive growth during a storage period of scion and rootstock to adjust a shipment time and maintain seedling quality grafted with stored scion and rootstock so as to prevent an influence to growth after rooting, thereby being usefully used storage and long-distance transportation of scion and rootstock.

Description

Method for Storing Fruit Vegetable Scion and Root Stock Seedlings

The present invention relates to a method for receiving and storing fruits and vegetables.

Grafting is to contact the nutrients of two plants so that the cambium is in close contact with each other, so that the physiological action is smoothly exchanged to form an independent individual. In grafting, the side that becomes the government is called the scion, and the side that becomes the donor is called the root stock. By such grafting, infectious disease prevention and continuous cultivation are possible, and by activating the dry resistance, moisture resistance, absorptivity and/or frost resistance of the root, it is possible to produce a seedling resistant to various environmental stresses. Therefore, it is possible to prolong the planting period, to prevent wounds occurring during transplantation of seedlings, and to reduce the amount of plant protection agents and fertilizers used.

In the case of vegetable seedlings in Korea, it relies on individual production of seedlings to expand the size of crops. Transition from the form of collectively or collectively producing seedlings of various high-quality crops to a form of producing seedlings by the process seedling method since the early 1990s. became According to this trend of specialization and division of labor, the seedling industry is expected to more than double in the past five years and reach 400 billion won in 2023. In addition, in accordance with the government's measures for the development of the seedling industry and the revision of the Seed Industry Act (introduction of the seedling quality labeling system, establishment of a base for production of excellent seedlings, vitalization of seedling distribution, etc.), the need for seedling of high quality seedlings is continuously increasing.

However, in the production of grafted seedlings, since special facilities and materials are used for grafting and seeding, seedling costs and labor are required, and quality may deteriorate or physiological disturbances may occur in the storage and transportation of grafted seedlings. In addition, due to recent climate change and the delay in planting of farmhouses due to abnormal climates, the grafting seedlings grow excessively and the planting time is missed, causing damage to the nursery. .

On the other hand, grafted seedlings are often used for cucumbers, tomatoes, watermelons, melons, peppers, etc. The planting time is almost constant, so a large number of grafted seedlings are required at one time. The supply of grafted seedlings is insufficient during the peak period. Therefore, in order to supply a large amount of grafted seedlings in accordance with a certain planting time, there is a need for a method capable of receiving and storing and long-distance transportation for the manufacture of grafted seedlings.

Accordingly, the present inventors completed the present invention by conducting research to develop a technology for controlling the shipping time of grafted seedlings and for exporting grafted seedlings.

Korean Patent Publication No. 10-2019-0030118

One object of the present invention is to prepare a seedling to be used as (a) receiving (接穗) and stock (臺木); And (b) to provide a method for receiving and storing fruits and vegetables, comprising the step of storing and receiving the stock in the dark.

One aspect of the present invention comprises the steps of (a) preparing a seedling to be used as a reception (接穗) and stock; and (b) receiving and storing the stock in the dark.

According to the method of receiving and storing a rootstock of the present invention, by suppressing excessive growth of grafted seedlings during the storage period of grafted seedlings to control the time of shipment and maintaining seedling qualities, the growth process after establishment of grafted seedlings can proceed normally, so that the The method of receiving and storing the seedlings can be usefully utilized for storage and long-distance transportation of the seedlings and roots used in the production of grafted seedlings.

The first step of the present invention is the step of preparing the seedlings to be used as reception and rootstock.

As used herein, the term "reception" refers to a breed to be propagated, and constitutes the above-ground part of the grafted seedlings. The recipient may be appropriately selected by those skilled in the art according to the purpose of grafting, the variety of the seedling, and the grafting method.

As used herein, the term "root" refers to a base seedling having roots after grafting. The stock may be, for example, a variety having strong disease resistance, cold resistance, dry resistance, etc. and exhibiting strong growth or special properties, and may be appropriately selected by those skilled in the art according to the purpose of grafting, the variety of the seedling and the grafting method, The present invention is not limited thereto.

The last step of the present invention is the step of storing the receipt and passage in the dark.

Even if grafted seedlings are prepared after storing the seedlings and stocks in the dark for up to 3 days, the seedling quality and growth level are similar to those of the grafted seedlings prepared using the seedlings and stocks that have not been stored in the dark. This can be delayed for 3 days.

According to one embodiment of the present invention, after step (a), before step (b), (a') it may further include the step of cutting the roots and roots of the stock.

Roots and rootstocks were cut and stored in darkness for 3 days, and grafted and established grafting and rootstock? Since the seedling quality and growth level are similar to those of grafted seedlings that have not been subjected to receiving and root cutting and dark storage, through these steps, as a result, the shipping time of grafted seedlings can be delayed for 3 days. In addition, since the roots are cut and stored, more seeds and roots can be stored per unit volume compared to the roots and roots that are not cut, so it is easy to receive and store and transport the roots.

The root cutting of the receiving and stock can be carried out by a general cutting method used in the art.

According to one embodiment of the present invention, the receiving and rootstock may be cucumbers, watermelons, tomatoes or peppers.

According to one embodiment of the present invention, the storage may be made at a temperature of 12 ℃.

According to one embodiment of the present invention, the storage may be made at a humidity of 80% or more.

Reception and storage of stocks may be made in, for example, a growth chamber that can maintain a constant temperature and humidity, but is not limited thereto.

For storage of the seedlings and roots, the seedlings and roots of the present invention are stored for up to 3 days in dark conditions of 12℃ and humidity of 80% or more, and thereafter, the grafted seedlings prepared by grafting the seedlings and roots are maintained at 5℃ and high humidity In the case of growing in an LED growing room, a sufficient amount of root is secured during the seeding period of grafted seedlings, enabling normal growth of grafted seedlings after planting.

As used herein, the term "grafted seedling" refers to a seedling formed so that the cambium of the seedling and the rootstock are in close contact with each other to adhere to each other so that the physiological action is smoothly exchanged, and is used in the same meaning as the grafted seedling or seedling.

The grafting method using the receiving and root of the present invention may be, for example, the cutting method, the double bonding method, the thin bonding method, the divided bonding method, the lingual bonding method, the intersecting method, the stitching method, the green folding method, the eye bonding method, or the joining method, but is limited thereto. It is not, and may be appropriately selected by those skilled in the art according to the variety and type of the receiving and stock.

For the performance of grafting using the receiving and rootstock of the present invention, for example, grafting knife, pruning shears, cutting saw, parafilm, vinyl tape, wire and sterile bleach, etc. grafting commonly used in grafting in the art A tool may be appropriately selected and used, but is not limited thereto.

According to the method of receiving and storing fruits and vegetables, it is possible to control the time of shipment by suppressing excessive growth during the period of receiving and storing the roots, while maintaining the seedling qualities of the grafted seedlings grafted with the stored plants and roots, thereby not affecting the growth after establishment. Therefore, it can be usefully utilized for reception and storage of trees and long-distance transportation.

1 is a photograph showing the foreground of (A) seedling seedlings, (B) storage temperature and humidity environment, and (C and D) storage scenery of seedlings.
2 is a photograph showing (A) seedling quality by number of days of storage and (B) seedling quality after grafting established during cucumber reception and root grafting.
3 is a photograph showing (A) seedling quality and (B) initial growth after planting at the time of planting of grafted seedlings grafted with cucumbers and rootstock by number of days of storage.
4 is a photograph showing (A) seedling quality by number of days of storage and (B) seedling quality after grafting established during watermelon reception and root grafting.
5 is a photograph showing (A) seedling quality and (B) initial growth after planting during planting of grafted seedlings grafted with watermelon and rootstock by number of days of storage.
6 is a photograph showing (A) seedling quality by number of days of storage and (B) seedling quality after grafting during tomato reception and root grafting.
7 is a photograph showing (A) seedling quality and (B) initial growth after planting at the time of planting of grafted seedlings grafted with tomatoes and rootstock by number of days of storage.
8 is a photograph showing (A) seedling quality by number of days of storage and (B) seedling quality after grafting established during red pepper reception and root grafting.
9 is a photograph showing (A) seedling quality and (B) initial growth after planting at the time of planting of grafted seedlings grafted with red pepper and rootstock by number of days of storage.

Hereinafter, the present invention will be described in more detail through one or more embodiments. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Example 1. Materials and method for preparing grafted seedlings

In order to prepare grafted seedlings of cucumber, watermelon, tomato and pepper, seedlings and rootstock varieties in Table 1 were grown in the vegetable and process seedling greenhouse of the National Institute of Horticultural and Herbal Science, and then each seedling and rootstock seedlings reaching the grafting stage were transferred to the rootless root (tray). ) or short roots, and stored for 1, 2, or 3 days in the growing phase at 12° C., dark conditions, and humidity of 80% or more.

division Receptionist breed stock varieties cucumber Jo Eun-baek teapot melanoma watermelon Sambok Honey immortality tomato TOTALANG DIA B blocking pepper cinnamon solid

After that, the seedlings and roots for each crop were grafted and grafted and grown in an LED growing room maintained at 25°C and high humidity to prepare cucumber, watermelon, tomato and pepper grafted seedlings (FIG. 1). And after grafting, plant height, stem diameter, number of leaf, leaf color (SPAD), leaf area, fresh weight, and dry weight of grafted seedlings were investigated. and compared.

Example 2. Confirmation of improvement in storage capacity of cucumbers and rootstocks

In order to confirm that the seedling quality of grafted seedlings is not affected by the dark storage or short root and dark storage of the seedlings before grafting and whether the storage period of the grafted seedlings can be substantially increased, according to Example 2-1, The growth characteristics of cucumber reception, rootstock and grafted seedlings according to short root treatment and/or dark storage period before storage were analyzed.

As a result, it was confirmed that grafted seedlings grafted with rootstocks and rootstocks stored in darkness exhibited similar seedling qualities to grafted seedlings grafted with unsaved rootstocks and rootstocks. On the other hand, as the number of days of short root treatment and storage increased, the seedling quality showed a tendency to slightly decrease, but it was investigated that there was no significant difference (Table 2 and FIG. 2).

process Save
period pasture
(cm/plant) Trunk diameter (mm/plant) ground game
(/plant) SPAD leaf area
(cm ² /plant) live weight
(g/plant) under building
(g/plant) tray 0 5.6 2.0 4.1 39.7 50.3 2.70 0.15 One 5.5 1.9 4.0 39.2 47.9 2.70 0.15 2 5.5 2.2 4.5 36.5 60.1 3.04 0.15 3 6.3 2.0 4.4 32.3 57.1 2.89 0.15 short root 0 5.9 1.9 4.0 40.1 51.4 2.66 0.15 One 5.4 1.8 3.9 41.9 38.0 2.56 0.14 2 4.6 2.0 4.3 37.0 48.2 2.59 0.14 3 5.0 2.0 4.3 37.6 51.6 2.77 0.14 Significance A * NS NS NS NS NS NS B NS NS NS NS NS NS NS A×B * NS NS NS NS NS NS

In addition, as a result of comparing the initial growth after planting of cucumber grafted seedlings treated with rootless root and dark storage, and cucumber grafted with short root and dark storage, it was confirmed that similar growth characteristics were exhibited between each treatment. By dark storage or storage in the dark for 2 to 3 days before grafting, it was confirmed that the efficiency and storability of the grafting operation could be improved, and the time of grafting seedlings could be adjusted (Table 3 and FIG. 3).

process process
number of days pasture
(cm/plant) Trunk diameter (mm/plant) ground game
(/plant) SPAD leaf area
(cm ² /plant) live weight
(g/plant) under building
(g/plant) tray 0 92.0 4.6 16.0 34.0 1,529 62.3 15.5 One 91.7 4.5 14.8 33.1 1,217 49.4 16.9 2 92.5 3.9 12.8 34.1 1,331 48.6 14.8 3 89.2 4.3 13.0 29.6 1,382 55.4 15.4 short root 0 99.7 4.2 15.0 32.3 1,648 66.1 15.8 One 86.2 4.5 12.8 36.3 1,074 43.8 15.9 2 93.1 4.3 13.2 31.8 1,571 60.2 15.5 3 94.1 4.1 12.8 28.0 1,590 62.4 14.7 Significance A NS NS NS NS * NS NS B NS NS *** ** ** * NS A×B NS NS NS NS NS NS NS

Example 3. Confirmation of watermelon reception and storage capacity improvement of rootstock

In order to confirm that the seedling quality of grafted seedlings is not affected by the dark storage of the seedlings before grafting and the dark storage, or short root and dark storage, the storage period of the grafted seedlings can be substantially increased, according to Example 2-1, The growth characteristics of watermelon reception, rootstock and grafted seedlings were analyzed according to short root treatment and/or dark storage period before storage.

As a result, it was confirmed that grafted seedlings grafted with rootstocks and rootstocks stored in darkness exhibited similar seedling qualities to grafted seedlings grafted with unsaved rootstocks and rootstocks. On the other hand, as the number of days of short root treatment and storage increased, the seedling quality showed a tendency to slightly decrease, but it was investigated that there was no significant difference (Table 4 and FIG. 4).

process process
number of days pasture
(cm/plant) Trunk diameter (mm/plant) ground game
(/plant) SPAD leaf area
(cm ² /plant) live weight
(g/plant) under building
(g/plant) tray 0 8.7 2.6 5.6 41.7 55.2 3.77 0.24 One 8.4 2.6 5.6 41.6 61.6 4.16 0.27 2 9.4 2.5 6.0 39.7 67.8 4.41 0.27 3 8.3 2.4 5.5 41.7 52.1 3.63 0.23 short root 0 7.6 2.5 5.4 43.0 60.0 3.50 0.24 One 5.8 2.3 5.1 41.6 43.1 2.83 0.20 2 6.5 2.5 5.4 42.9 58.0 3.56 0.23 3 6.9 2.3 5.5 42.8 54.3 3.25 0.21 Significance A ** NS NS NS NS * NS B NS NS NS NS NS NS NS A×B NS NS NS NS NS NS NS

However, as a result of comparing the initial growth after planting of short root and stored watermelon grafted seedlings, it was confirmed that the growth decreased as the number of days of short root treatment and storage increased (Table 5 and FIG. 5).

process process
number of days pasture
(cm/plant) Trunk diameter (mm/plant) ground game
(/plant) SPAD leaf area
(cm ² /plant) live weight
(g/plant) under building
(g/plant) tray 0 108.3 4.2 19.0 38.0 1,009.6 43.58 4.02 One 101.4 3.4 19.2 38.2 954.5 39.94 3.42 2 88.8 3.8 17.6 40.5 756.8 32.76 2.78 3 92.8 3.4 16.4 40.8 825.1 36.78 3.22 short root 0 104.2 3.6 19.2 35.3 957.0 40.90 3.80 One 77.1 3.1 15.2 38.6 668.7 27.64 2.30 2 91.1 3.7 15.8 40.7 794.7 35.68 3.20 3 93.4 3.9 14.8 43.4 820.7 37.76 3.46 Significance A ** NS *** NS ** NS NS B *** ** *** * *** *** *** A×B ** NS * NS ** *** ***

Example 4. Confirmation of improvement in storage capacity of tomatoes and roots

In order to confirm that the seedling quality of grafted seedlings is not affected by the dark storage of the seedlings before grafting and the dark storage, or short root and dark storage, the storage period of the grafted seedlings can be substantially increased, according to Example 2-1, The growth characteristics of tomato reception, rootstock and grafted seedlings according to the short root treatment and/or dark storage period before storage were analyzed.

As a result, it was confirmed that grafted seedlings grafted with rootstocks and rootstocks stored in darkness exhibited similar seedling qualities to grafted seedlings grafted with unsaved rootstocks and rootstocks. On the other hand, as the number of days of short root treatment and storage increased, the seedling quality tended to slightly decrease, but it was investigated that there was no significant difference (Table 6 and FIG. 6).

process process
number of days pasture
(cm/plant) Trunk diameter (mm/plant) ground game
(/plant) SPAD leaf area
(cm ² /plant) live weight
(g/plant) under building
(g/plant) tray 0 11.4 3.7 6.2 28.0 43.2 2.70 0.15 One 9.5 3.4 6.8 28.1 43.4 2.51 0.15 2 8.6 3.4 6.2 30.2 37.1 2.14 0.12 3 9.0 3.4 6.3 29.0 36.7 2.24 0.15 short root 0 9.2 3.3 6.3 29.5 42.1 2.18 0.12 One 8.9 3.2 6.5 29.7 49.3 2.29 0.13 2 9.2 3.2 6.7 28.8 51.2 2.33 0.12 3 8.8 3.3 6.5 30.6 47.4 2.16 0.14 Significance A NS *** NS NS * NS NS B ** NS NS NS NS NS NS A×B * NS NS NS NS NS NS

In addition, as a result of comparing the initial growth after planting of tomato grafted seedlings treated with rootless root and dark storage, and tomato grafted seedlings treated with short root and dark storage, it was confirmed that similar growth characteristics were exhibited between each treatment. By dark storage or storage in the dark for 2 to 3 days before grafting, it was confirmed that the efficiency and storability of the grafting operation could be improved, and the time of grafting seedlings could be controlled (Table 7 and FIG. 7).

process process
number of days pasture
(cm/plant) Trunk diameter (mm/plant) ground game
(/plant) SPAD leaf area
(cm ² /plant) live weight
(g/plant) under building
(g/plant) tray 0 57.9 5.8 15.2 46.9 1,383.7 80.33 6.58 One 62.4 5.5 14.0 46.3 1,308.2 80.07 5.75 2 57.2 5.6 15.0 47.6 1,334.2 80.73 6.14 3 50.8 5.7 14.8 47.1 1230.2 75.17 5.80 short root 0 57.3 6.2 14.8 46.4 1,360.8 81.57 6.57 One 60.9 5.9 14.6 46.9 1,378.9 79.97 6.10 2 55.3 6.0 14.0 47.7 1,301.5 76.89 5.92 3 54.9 5.7 13.4 48.6 1,135.2 72.37 5.82 Significance A NS NS NS NS NS NS NS B ** NS NS NS ** NS * A×B * NS NS NS NS NS NS

Example 5. Confirmation of improvement in storage capacity of red pepper reception and rootstock

In order to confirm that the seedling quality of grafted seedlings is not affected by the dark storage of the seedlings before grafting and the dark storage, or short root and dark storage, the storage period of the grafted seedlings can be substantially increased, according to Example 2-1, The growth characteristics of red pepper reception, root stock and grafted seedlings were analyzed according to the short root treatment and/or dark storage period before storage.

As a result, it was confirmed that grafted seedlings grafted with rootstocks and rootstocks stored in darkness exhibited similar seedling qualities to grafted seedlings grafted with unsaved rootstocks and rootstocks. On the other hand, as the number of days of short root treatment and storage increased, the seedling quality showed a tendency to slightly decrease, but it was investigated that there was no significant difference (Table 8 and FIG. 8).

process process
number of days pasture
(cm/plant) Trunk diameter (mm/plant) ground game
(/plant) SPAD leaf area
(cm ² /plant) live weight
(g/plant) under building
(g/plant) tray 0 10.3 2.6 9.6 25.8 39.9 1.62 0.13 One 10.1 2.5 8.8 25.7 41.0 1.73 0.19 2 11.5 2.6 9.4 23.5 47.8 1.92 0.14 3 10.5 2.5 9.1 25.3 45.6 1.75 0.17 short root 0 7.8 2.3 6.9 25.5 23.8 0.87 0.07 One 8.6 2.4 8.1 24.6 29.5 1.12 0.16 2 8.6 2.5 8.4 22.2 35.2 1.22 0.10 3 9.2 2.5 7.9 25.3 33.5 1.24 0.12 Significance A *** * *** NS *** *** *** B ** NS * * *** *** *** A×B NS NS *** NS NS NS NS

In addition, as a result of comparing the initial growth after planting of red pepper grafted seedlings treated with unrooted root and dark storage treatment, and red pepper grafted seedlings treated with short root and dark storage treatment, it was confirmed that similar growth characteristics were exhibited between each treatment. By dark storage or storage in the dark for 2 to 3 days before grafting, it was confirmed that the efficiency and storability of the grafting operation could be improved, and the time of grafting seedlings could be adjusted (Table 9 and FIG. 9). However, it was confirmed that the time required for rooting was relatively increased in the case of short root treatment.

process process
number of days pasture
(cm/plant) Trunk diameter (mm/plant) ground game
(/plant) SPAD leaf area
(cm ² /plant) live weight
(g/plant) under building
(g/plant) tray 0 28.4 4.2 45.8 39.6 376.5 15.04 1.84 One 31.1 4.8 48.2 41.8 419.4 17.52 2.24 2 29.3 4.3 40.2 39.2 389.9 15.44 1.88 3 28.8 4.4 39.6 39.2 391.8 15.14 1.90 short root 0 32.0 5.1 59.0 47.7 552.7 22.18 2.76 One 30.2 5.0 51.6 46.3 457.9 19.62 2.40 2 30.5 5.1 52.4 45.5 499.7 20.44 2.48 3 29.5 5.5 50.2 47.6 510.1 21.58 2.74 Significance A * *** *** *** *** *** *** B NS NS * NS NS NS NS A×B * * NS NS * * *

So far, the present invention has been looked at focusing on the embodiments thereof. Those of ordinary skill in the art to which the present invention pertains will understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments are to be considered in an illustrative rather than a restrictive sense. The scope of the present invention is indicated in the claims rather than the foregoing description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (1)

(a) preparing seedlings to be used as reception and stock;
(a') cutting the roots and roots of the stock; and
(b) receiving and storing the stock for 1 to 3 days at a temperature of 12 ° C., a humidity of 80% or more, and in the dark
A method of receiving and storing stock of cucumbers, watermelons, tomatoes or peppers comprising a.

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