KR20210151575A - Composition for Preventing Hair Loss or Promoting Hair Growth Comprising a Culture of Aureobasidium pullulans - Google Patents

Abstract

The present invention relates to a composition for preventing hair loss or promoting hair growth, comprising a culture of an Aureobasidium pullulans strain. More specifically, the present invention relates to a composition for preventing hair loss or promoting hair growth comprising an Aureobasidium pullulans strain which promotes the growth of dermal papilla cells and promotes vascular endothelial growth factor expression in the dermal papilla cells, a culture of the strain, a lysate of the strain, an extract of the strain, an extract of the culture or an extract of the lysate, as an active component.

Description

A composition for preventing hair loss or promoting hair growth comprising a culture of Aureobasidium pullulans strain {Composition for Preventing Hair Loss or Promoting Hair Growth Comprising a Culture of Aureobasidium pullulans}

The present invention relates to a composition for preventing hair loss or promoting hair growth, comprising a culture of Aureobasidium pullulans strain, and more specifically, promotes the growth of dermal papilla cells, and vascular endothelial growth factor of dermal papilla cells Aureobasidium pullulans strain that promotes expression, the culture of the strain, the lysate of the strain, the extract of the strain, the extract of the culture or the extract of the lysate as an active ingredient In a composition for preventing hair loss or promoting hair growth it's about

Each hair of the human body operates and proceeds in an independent cycle, and as a specific cycle, it grows and falls out while going through the anagen, catagen, and telogen phases. In general, the hair loss phenomenon can be divided into a general telogen hair loss phenomenon, in which hair falls out in a telogen state, and a growth phase hair loss phenomenon, which is an abnormal hair loss phenomenon, in particular, the latter is referred to as alopecia.

The cause of hair loss is not yet clearly known, but genetic causes are largely located, and it is known that it is caused by poor blood circulation in the scalp, excessive action of male hormones, excessive sebum secretion, aging, stress, etc. Various hair loss prevention and hair growth agents have been developed according to these various causes, but most of the hair growth agents show some effect in preventing hair loss, but the effects related to hair growth were not very satisfactory. No. describes the hair growth effect of a composition essentially containing minoxidil, an emollient and a skin retention agent, but the prior art, including the above patent, cannot predict whether the hair growth effect is exhibited due to any active ingredient, and is still satisfactory. There is a problem in that it does not show an effective effect.

On the other hand, as chemical raw materials and animal raw materials are avoided in recent years, the need to find environmentally friendly bio-efficacious materials is emerging. Unlike petroleum and water, microbial resources are classified as sustainable resources in that they can be reproduced, and since they use the unique characteristics of microorganisms adapted to various environments, research and industrial application possibilities are very high. Accordingly, there is a need to develop a composition containing an active ingredient derived from a microorganism and exhibiting an excellent hair loss prevention or hair growth promoting effect.

Aureobasidium pullulans ( Aureobasidium pullulans ) is one of the microorganisms of the genus Aureobasidium, commonly referred to as black yeast. Microorganisms of the genus Aureobasidium are microorganisms that produce alpha-glucan (α-glucan) and beta-glucan (β-glucan), and are found in soil and air. radionuclides), so it can survive/grow and has excellent adaptability to the environment.

Among them, Aureobasidium pullulans is a microorganism that produces alpha-glucan, pullulan, and the produced pullulan is linked by an α-1,4 glycosidic bond. may include maltotriose, a trisaccharide composed of three glucose molecules (see Yurlova et al., Stud Mycol , 61:39-49, 2008; https://en.wikipedia.org/wiki/Pullulan ) .

Antioxidant and atopy skin protection effects of pullulan, a substance produced by the Aureobasidium pullulans strain, are known, but the hair loss prevention or hair growth promoting effect is not known.

Specifically, the Aureobasidium pullulans strain, the culture of the strain, the lysate of the strain, the extract of the strain, the extract of the culture or the extract of the lysate is applied to the skin, etc. A study on the effect of improving blood circulation, And there are no studies on the effect of preventing hair loss or promoting hair growth through this.

Under this technical background, the present inventors Aureobasidium pullulans GJW strain (Accession No. KCCM12142P), the culture of the strain, the lysate of the strain, the extract of the strain, the extract of the culture or the extract of the lysate of the dermal papilla cells The present invention has the effect of promoting growth and promoting the expression of vascular endothelial growth factor in dermal papilla cells, and discovering that the culture can be used as an active ingredient in a composition for preventing hair loss or promoting hair growth through these effects was completed.

Republic of Korea Patent Publication No. 10-1467568 (Announcement date of 2014.12.02)

The present invention has been devised to solve the above problems, and an object of the present invention is an Aureobasidium pullulans strain, a culture of the strain, a lysate of the strain, an extract of the strain, an extract or lysate of the culture It is to provide a composition that promotes the growth of dermal papilla cells and promotes the expression of vascular endothelial growth factors in dermal papilla cells, thereby exhibiting an excellent effect of preventing hair loss or promoting hair growth by including the extract of as an essential active ingredient.

In order to achieve the above object of the present invention, the present invention is an Aureobasidium pullulans strain, the culture of the strain, the lysate of the strain, the extract of the strain, the extract of the culture or the extract of the lysate as an active ingredient It provides a composition for preventing hair loss or promoting hair growth, including.

In addition, the present invention provides an Aureobasidium pullulans strain as an active ingredient in the preparation of a composition for preventing hair loss or promoting hair growth, a culture of the strain, a lysate of the strain, an extract of the strain, an extract or lysate of the culture Provided is a use of the extract of

According to the present invention, the composition comprising the Aureobasidium pullulans strain, the culture of the strain, the lysate of the strain, the extract of the strain, the extract of the culture or the extract of the lysate as an essential active ingredient is the growth of dermal papilla cells and promotes the expression of vascular endothelial growth factor in dermal papilla cells, thereby providing an excellent effect of preventing hair loss or promoting hair growth.

1 shows that the culture extract of the Aureobasidium pullulans strain according to an embodiment of the present invention has the effect of increasing the growth (cell viability) of human dermal papilla cells. will be.
Figure 2 shows that the culture extract of the Aureobasidium pullulans strain according to an embodiment of the present invention has the effect of increasing the dermal endothelial growth factor (Vascular Endothelial Growth Factor, VEGF) expression of the dermal papilla cells.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In general, the nomenclature used herein is those well known and commonly used in the art.

Hereinafter, the present invention will be specifically described.

As used herein, the term "about" used to express length, area, volume, time (period), concentration, content, temperature, number, percent (%), multiple, etc., means that the numerical value or range of values is allowed to be up to 10%. It means that there is an error.

The present invention in one aspect, Aureobasidium pullulans ( Aureobasidium pullulans ) strain, a culture of the strain, a lysate of the strain, an extract of the strain, an extract of the culture or an extract of the lysate as an active ingredient, It relates to a composition for preventing hair loss or promoting hair growth.

The composition may be a pharmaceutical composition for preventing hair loss or promoting hair growth having a use for preventing or treating alopecia.

The composition may be a cosmetic composition for preventing hair loss or promoting hair growth having a use for improving alopecia.

In another aspect, the present invention prevents hair loss or promotes hair growth of the Aureobasidium pullulans strain contained in the composition, the culture of the strain, the lysate of the strain, the extract of the strain, the extract of the culture or the extract of the lysate It's about use.

In another aspect, the present invention provides a composition comprising an Aureobasidium pullulans strain, a culture of the strain, a lysate of the strain, an extract of the strain, an extract of the culture or an extract of the lysate as an active ingredient (preferably relates to a method for preventing hair loss or promoting hair growth, comprising the step of (topically) applying or administering a cosmetic composition or pharmaceutical composition) to a subject (a mammal such as a human).

In another aspect, the present invention provides an Aureobasidium pullulans strain, a culture of the strain, a lysate of the strain, for producing a pharmaceutical formulation having an effect of preventing or treating alopecia or a cosmetic formulation having an effect of improving alopecia , it relates to the use (use) of the extract of the strain, the extract of the culture or the extract of the lysate.

The pharmaceutical preparation or cosmetic preparation may be a hair growth/hair growth promoter (improving agent) or an agent for preventing hair loss.

In another aspect, in the preparation of a pharmaceutical composition or cosmetic composition for hair growth or hair growth, the present invention provides an Aureobasidium pullulans strain, a culture of the strain, a lysate of the strain, an extract of the strain, an extract of the culture or to the use (use) of the extract of the lysate.

The alopecia (or hair loss disease) is alopecia areata, hereditary androgenetic alopecia, telogen alopecia, traumatic alopecia, alopecia on the hair, pressure alopecia, genital alopecia, alopecia pityroides, syphilitic alopecia, seborrhea, depending on the form, symptom, or cause. It may be at least one selected from alopecia, symptomatic alopecia, non-scarring alopecia, alopecia scar, and alopecia congenital.

As the Aureobasidium pullulans strain, any commonly known strain can be used, but it is preferable to use the Aureobasidium pullulans GJW (Aureobasidium pullulans GJW) strain isolated and deposited by the present applicant.

Specifically, under the Budapest Treaty, the applicant deposited with the Korean Culture Center of Microorganisms (KCCM) on October 31, 2017 and was given an accession number of KCCM12142P using the Aureobasidium pullulans GJW strain. it is preferable

In one embodiment of the present invention, the culture of the Aureobasidium pullulans strain is a culture product comprising some or all of the substances contained in the culture medium or the culture medium, including the culture medium or culture medium, and means its concentrate. For example, it may refer to a metabolite or secretion resulting from the culture of the strain, or a lysate thereof, and the strain itself may also be included in the culture.

The culture may be cultured in a culture medium containing all of a natural medium, a synthetic medium, or a selective medium, and in particular, may be cultured in a culture medium containing potato extract and dextrose. The culture is not limited in its form or formulation, and may be, for example, liquid or solid.

In one embodiment of the present invention, the culture of the Aureobasidium pullulans strain is centrifuged, washed with sterile physiological saline, dispersed in a solvent, such as sterile oil, and lyophilized to freeze-dried powder It can be prepared and used in the form.

In one embodiment of the present invention, the lysate of the Aureobasidium pullulans strain may be a product obtained by crushing the strain itself by chemical or physical force.

In one embodiment of the present invention, the extract is a product obtained by extracting the strain itself, the culture of the strain, the lysate of the strain, or a mixture thereof regardless of the extraction method, the extraction solvent, the extracted component or the form of the extract. It can mean, and is a broad concept that includes all substances that can be obtained by processing or processing in other ways after extraction. Specifically, the extract may be an ethanol fraction or an ethanol precipitate.

In one embodiment of the present invention, the composition is an Aureobasidium pullulans strain as an active ingredient, a culture of the strain, a lysate of the strain, an extract of the strain, an extract of the culture or an extract of the lysate of the composition It may be included in an amount of 0.001 to 30% by weight based on the total weight. Specifically, the active ingredient is included in an amount of 0.001% by weight or more, 0.01% by weight or more, 0.1% by weight or more, 0.5% by weight or more, 1.0% by weight or more, 1.5% by weight or more, or 2.0% by weight or more, based on the total weight of the composition. and may be included in an amount of 30% by weight or less, 25% by weight or less, 20% by weight or less, 15% by weight or less, 10% by weight or less, or 5.0% by weight or less. If the content of the active ingredient in the composition is less than 0.001% by weight, there is little effect in preventing hair loss or promoting hair growth (eg, promoting growth of dermal papilla cells and promoting vascular endothelial growth factor expression in dermal papilla cells), and the content of the active ingredient is 30 Exceeding the weight percent may affect formulation stability.

On the other hand, growth factors are secreted from cells as substances that can stimulate the growth, proliferation, and differentiation of cells. There are many types of growth factors and steroid hormones, and they are very important as they play a role in regulating various cells. In addition, growth factors act as signaling molecules between cells and bind to specific receptors on the surface of specific cells. Types of growth factors include Keratinocyte Growth Factor (KGF) and Vascular Endothelial Growth Factor (VEGF). are giving

Keratinocyte growth factor is one of fibroblast growth factors and is also called fibroblast growth factor-7 (FGF-7). It promotes the growth of hair and skin cells by enhancing skin elasticity, and activates dermal papilla cells. The dermal papilla cells are cells located at the bottom of the hair follicle and are involved in the generation and growth of hair. The proliferation of these dermal papilla cells is viewed as a doubling time of 48 hours, and the cell activity of effective substances is also identified through the proliferation of dermal papilla cells. In addition, the keratinocyte growth factor increases the synthesis of hyaluronic acid in the keratinocytes and promotes the biosynthesis of collagen and elastin in the dermal layer to regulate the hair cycle to maintain the hair condition and prevent hair loss.

In addition, vascular endothelial growth factor promotes cell division and migration by increasing plasma protein permeation of capillaries, promotes neovascularization, participates in blood vessel maintenance, and increases capillary size and distribution of hair follicles to increase hair thickness and distribution and promotes hair growth.

The keratinocyte growth factor, fibroblast growth factor, and vascular endothelial growth factor are all factors related to the growth of dermal papilla cells, and increasing the expression of these growth factors is important for maintaining the growth of dermal papilla cells.

The Aureobasidium pullulans strain, preferably the Aureobasidium pullulans GJW strain (Accession No. KCCM12142P), the culture of the strain, the lysate of the strain, the extract of the strain, the extract of the culture or the extract of the lysate can promote the growth of dermal papilla cells, can promote the expression of growth factors, preferably vascular endothelial growth factors, of dermal papilla cells, more preferably, vascular endothelial growth factors of dermal papilla cells derived from human skin tissue promote the expression of

As described above, the composition of the present invention may be a pharmaceutical composition, and the pharmaceutical composition may include a pharmaceutically acceptable carrier. The pharmaceutically acceptable carrier is lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil and the like. It also includes diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, surfactants, and the like.

The pharmaceutical composition may be formulated in the form of oral preparations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups and aerosols, parenteral preparations, injections, and topical administration preparations according to conventional methods.

Among oral formulations, oral solid formulations include tablets, pills, powders, granules, capsules, etc., and these solid formulations include at least one excipient, for example, starch, calcium carbonate, sucrose or lactose, gelatin. and the like, and may include a lubricant such as magnesium stearate and talc. Among oral formulations, oral liquid formulations include suspensions, solutions, emulsions, syrups, and the like, and may include water, diluents such as liquid paraffin, wetting agents, sweeteners, fragrances, preservatives, and the like. Parenteral preparations include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, and freeze-dried preparations. Non-aqueous solvents and suspensions include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injections such as ethyl oleate. possible esters and the like.

In addition, the pharmaceutical composition may be administered to mammals, including or not including humans, by various routes, for example, by oral, intravenous, intramuscular, or subcutaneous injection.

The compositions of the present invention may be administered topically. Topical administration means direct application of an active ingredient or composition (such as a composition for external application to the skin) to the skin and/or hair. The topical composition of the present invention may be in the form of a solution, lotion, plaster, cream, ointment, liposome, spray, gel, foam, roller stick, or any other formulation routinely used in dermatology. have.

In the composition, use, and method of the present invention, the dosage of the active ingredient varies depending on the patient's condition and weight, the degree of disease, drug form, administration route, period, etc., but may be appropriately selected depending on the case. The administration may be administered once or divided into several times a day.

As described above, the composition of the present invention may be a cosmetic composition, and the cosmetic composition is the Aureobasidium pullulans strain, preferably the Aureobasidium pullulans GJW strain (Accession No. KCCM12142P), and the culture of the strain Water, the lysate of the strain, the extract of the strain, the extract of the culture or the extract of the lysate as an active ingredient, according to a conventional cosmetic preparation method, can be prepared in various forms. Specifically, the cosmetic composition is a hair tonic, hair cream, hair lotion, hair shampoo, hair conditioner, hair conditioner, hair spray, hair aerosol, pomade, powder, gel, hair pack, hair treatment, eyebrow hair growth agent, eyelash hair growth agent, eyelashes It may be prepared in the form of nutrients or ointments.

In addition, the cosmetic composition may include conventional adjuvants such as stabilizers, solubilizers, vitamins, pigments and fragrances commonly used in the field of cosmetic compositions, and may include a cosmetically acceptable carrier.

Cosmetically acceptable carriers include, but are not limited to, purified water, oils, waxes, fatty acids, fatty alcohols, fatty acid esters, surfactants, humectants, thickeners, viscosity stabilizers, chelating agents, buffers, preservatives, lower alcohols, and the like. it is not If necessary, a moisturizer, an anti-inflammatory agent, an antibacterial agent, an antifungal agent, a vitamin, a sunscreen, an antibiotic, a perfume, and a dye may be included.

{Example}

Hereinafter, the present invention will be described in detail by way of Examples. However, the following examples are only examples to help the overall understanding of the present invention, and the content of the present invention is not limited to the following examples.

<Example 1> Preparation of culture extract of Aureobasidium pullulans strain

Aureobasidium pullulans GJW (Aureobasidium pullulans GJW) strain [Accession No. KCCM12142P] identified and deposited by the present applicant was obtained from the Korean Culture Center of Microorganisms (KCCM), a depository institution.

The obtained strain was suspended in a culture medium (potato extract 4g/L, dextrose 20g/L) in a volume of 1% (v/v) (PBS [phosphate buffered saline] concentration: 1.0 x 10 ⁶ cfu (colony) forming unit)/ml), and cultured with shaking at 30° C./120 rpm for 5 days using a shaking incubator to obtain a culture. Thereafter, the culture was centrifuged to separate cells and a culture solution (supernatant), and only the culture solution was collected. Ethanol was added to the collected culture solution in the same volume as the culture solution and allowed to react for 1 day at 4 ° C. A Black Yeast Culture Extract was obtained.

<Test Example 1> Confirmation of the growth effect of human dermal papilla cells treated with the culture extract of Aureobasidium pullulans strain

In order to confirm the effect of the culture extract (Black Yeast Culture Extract) of the Aureobasidium pullulans strain according to Example 1 on the growth of human dermal papilla cells, the extract was treated on human dermal papilla cells provided from the Department of Dermatology, Dankook University. After that, the cell viability was checked.

Specifically, using DMEM (Dulbecco's Modified Eagle Media, HyClone GE Healthcare) medium containing 10% Fetal Bovine Serum (Cambrex), 1% antibiotics, and 0.1% fungizone, human dermal papilla Cells were seeded in a 96-well plate in a volume of 2 × 10 ² cells/100 μl per well, and cultured (incubator culture) at _{37° C. and 5% CO 2 humid conditions for 72 hours.} After 24 hours, the medium used for culture was removed from each well of the 96-well plate, DPBS (Dulbecco's Phosphate Buffer Saline, Sigma Aldrich, USA) was added to each well and washed twice, and then the medium without FBS ( serum free DMEM) was added.

Then, a treatment group was prepared by adding the extract according to Example 1 so that the final concentrations per well of the 96-well plate were 10ppm, 20ppm, and then _{cultured in an incubator (37°C/5% CO 2} wet condition) for 72 hours. Then, after treatment with CCK-8 reagent (Cell Counting Kit-8, CK04-11, Dojindo Molecular Technologies, Inc, MA, USA) for 2 hours, absorbance at a wavelength of 450 nm was measured with a microplate reader. to confirm the cell viability.

In the positive control group, DMEM ('5% FBS') containing 5% (v/v) FBS was used instead of the extract according to Example 1. In addition, the untreated group not treated with the extract or FBS according to Example 1 was prepared as a control group.

The test was repeated three times, and the obtained test results were statistically processed and shown in FIG. 1 .

As can be seen in Figure 1, the extract treatment group according to Example 1 improved the growth of human dermal papilla cells compared to the control group at extract concentrations of 10ppm and 20ppm to the level of 25.91% and 26.87%.

As a result, according to the test results of FIG. 1, the culture extract of the Aureobasidium pullulans strain according to Example 1 exhibited excellent dermal papilla cell growth promoting activity, and it was found that there was an excellent hair loss prevention or hair growth promoting effect.

<Test Example 2> Confirmation of the effect of increasing the expression of vascular endothelial growth factor in human dermal papilla cells by the culture extract of the Aureobasidium pullulans strain

In order to determine the effect of the culture extract of the Aureobasidium pullulans strain according to Example 1 on the expression of vascular endothelial growth factor (VEGF) in the skin, human dermal papilla cells were treated with the extract The expression level of vascular endothelial growth factor was confirmed.

After seeding human dermal papilla cells using DMEM medium containing 10% FBS, 1% antibiotics and 0.1% fungal zone in the wells of a 6-well plate so as to become 3 × 10 ^{5 cells/ml, the extract according to Example 1 was added} A treatment group was prepared by treating human dermal papilla cells seeded in a 6-well plate at a concentration of 20 ppm, and then cultured for 48 hours. ) was used to confirm.

In the positive control group, 10 μM Minoxidil (MNX) was used instead of the extract according to Example 1. In addition, the untreated group that was not treated with the extract according to Example 1 or Minoxidil was prepared as a control group.

The test was repeated three times, and the obtained test results were statistically processed and shown in FIG. 2 .

As can be seen in Figure 2, the extract-treated group according to Example 1 (concentration of 20 ppm) expressed the vascular endothelial growth factor of human dermal papilla cells at a level about 1.94 times higher than that of the control group, and compared to the 10 μM Minoxidil-treated group, which is a positive control group. The vascular endothelial growth factor of human dermal papilla cells was expressed at a level of about 2.28 times.

As a result, according to the test results of FIG. 2 , the culture extract of the Aureobasidium pullulans strain according to Example 1 had the effect of significantly increasing the vascular endothelial growth factor expression level of dermal papilla cells, and thus the plasma of capillary blood Increases protein permeability to promote cell division and migration, promotes new blood vessel formation, participates in blood vessel maintenance, and increases the size and distribution of capillaries in hair follicles to increase hair thickness and distribution and promote hair growth Could know.

Statistical processing

For statistical analysis, the t-test test method was used to test the significance between the two groups, and compared with the control group, * P <0.05, ** P <0.01, *** P <0.001 was considered a valid difference.

Aureobasidium pullulans strain according to the present invention, the culture of the strain, the lysate of the strain, the extract of the strain, the extract of the culture or the composition comprising the extract of the lysate as an active ingredient are various external preparations/cosmetics/medicines It can be commercialized as a formulation, and an appropriate content ratio can be controlled in consideration of the functionality, cost, and other conditions of the product to be implemented.

[Formulation Example 1] Tablet

According to the composition shown in Table 1, in a conventional manner, the Aureobasidium pullulans strain according to the present invention, the culture of the strain, the lysate of the strain, the extract of the strain, the extract of the culture or the extract of the lysate are effective Tablets containing as an ingredient were prepared.

Ingredients content (wt%) Aureobasidium pullulans strain according to the present invention, the culture of the strain, the lysate of the strain, the extract of the strain, the extract of the culture or the extract of the lysate 0.001 to 30 vitamin C 2 magnesium stearate One Carboxymethylcellulose calcium 2 silicon dioxide One crystalline cellulose remaining amount

[Formulation Example 2] Ointment

According to the composition described in Table 2, the Aureobasidium pullulans strain according to the present invention, the culture of the strain, the lysate of the strain, the extract of the strain, the extract of the culture or the extract of the lysate according to the present invention are effective according to the composition described in Table 2 An ointment containing as a component was prepared.

Ingredients content (wt%) Aureobasidium pullulans strain according to the present invention, the culture of the strain, the lysate of the strain, the extract of the strain, the extract of the culture or the extract of the lysate 0.001 to 30 sorbitol 9 Caprin/Caprylic Triglyceride 9 liquid paraffin 9 Sorbitan Sesquioleate 6 Octyldodeces-25 9 Cetylethylhexanoate 9 squalane One salicylic acid One glycerin 15 Purified water remaining amount

[Formulation Example 3] Lotion

According to the composition described in Table 3, the Aureobasidium pullulans strain according to the present invention, the culture of the strain, the lysate of the strain, the extract of the strain, the extract of the culture or the extract of the lysate according to the present invention are effective according to the composition described in Table 3 A lotion containing as an ingredient was prepared.

Ingredients content (wt%) Aureobasidium pullulans strain according to the present invention, the culture of the strain, the lysate of the strain, the extract of the strain, the extract of the culture or the extract of the lysate 0.001 to 30 EDTA 2Na 0.2 Hydroxyethyl Cellulose 0.5 mineral oil 5 cetostearyl alcohol 2 Preservatives, Flavors and Colors appropriate amount Purified water remaining amount

Although the present invention has been described with reference to the above-mentioned preferred embodiments, various modifications and variations are possible without departing from the spirit and scope of the invention. Accordingly, it is intended that the appended claims cover such modifications and variations as fall within the scope of the present invention.

Korea Microorganism Conservation Center (Overseas) KCCM12142P 20171031

Claims (13)

Aureobasidium pullulans ( Aureobasidium pullulans ) strain, the culture of the strain, the lysate of the strain, the extract of the strain, the extract of the culture or the extract of the lysate as an active ingredient, preventing hair loss or promoting hair growth composition. According to claim 1,
The Aureobasidium pullulans strain is an Aureobasidium pullulans GJW (Aureobasidium pullulans GJW) strain deposited with accession number KCCM12142P, a pharmaceutical composition for preventing hair loss or promoting hair growth. According to claim 1,
The composition is a pharmaceutical composition for preventing or treating alopecia, preventing hair loss or promoting hair growth. 4. The method of claim 3,
The alopecia is alopecia areata, hereditary androgenetic alopecia, telogen alopecia, traumatic alopecia, alopecia on the hair, pressure alopecia, genital alopecia, alopecia pityroides, syphilitic alopecia, seborrheic alopecia, symptomatic alopecia, non-scarring alopecia, scar alopecia And at least one selected from congenital alopecia, a pharmaceutical composition for preventing hair loss or promoting hair growth. According to claim 1,
The composition is a pharmaceutical composition for preventing hair loss or promoting hair growth, which promotes the growth of dermal papilla cells. According to claim 1,
The composition promotes the expression of vascular endothelial growth factor in dermal papilla cells, a pharmaceutical composition for preventing hair loss or promoting hair growth. 7. The method according to any one of claims 1 to 6,
The composition is a composition for external application to the skin, a pharmaceutical composition for preventing hair loss or promoting hair growth. Aureobasidium pullulans ( Aureobasidium pullulans ) strain, the culture of the strain, the lysate of the strain, the extract of the strain, the extract of the culture or the extract of the lysate as an active ingredient, a cosmetic for preventing hair loss or promoting hair growth composition. 9. The method of claim 8,
The Aureobasidium pullulans strain is an Aureobasidium pullulans GJW (Aureobasidium pullulans GJW) strain deposited with accession number KCCM12142P, a cosmetic composition for preventing hair loss or promoting hair growth. 9. The method of claim 8,
The composition promotes the growth of dermal papilla cells, a cosmetic composition for preventing hair loss or promoting hair growth. 9. The method of claim 8,
The composition promotes the expression of vascular endothelial growth factor in dermal papilla cells, a cosmetic composition for preventing hair loss or promoting hair growth. 12. The method according to any one of claims 8 to 11,
The composition is a composition for external application to the skin, a cosmetic composition for preventing hair loss or promoting hair growth. 13. The method of claim 12,
The formulation of the composition includes hair tonic, hair cream, hair lotion, hair shampoo, hair conditioner, hair conditioner, hair spray, hair aerosol, pomade, powder, gel, hair pack, hair treatment, eyebrow hair growth agent, eyelash hair growth agent, eyelash nutrition agent or An ointment, a cosmetic composition for preventing hair loss or promoting hair growth.

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