KR20210132794A - A food composition including atage-905 collagen and ellastin mixture showing anti-senescent activity - Google Patents

Abstract

The present invention relates to an anti-aging food composition containing an ATAGE-905 collagen elastin mixture and to an anti-aging food composition having effects such as the enhancement of cell activity, the inhibition of collagenase expression, the inhibition of elastinase activity, and the promotion of differentiation of keratinocytes by adding vitamin, a Centella asiatica extract, pollen, and the like to fish collagen and elastin. The anti-aging food composition of the present invention can maximize an anti-aging function through synergy action between the compositions, and can be provided in the form of food, thereby having the effects of being more convenient and sustainable.

Description

A FOOD COMPOSITION INCLUDING ATAGE-905 COLLAGEN AND ELLASTIN MIXTURE SHOWING ANTI-SENESCENT ACTIVITY

The present invention relates to an anti-aging food composition comprising a mixture of ATAGE-905 collagen and elastin, and by adding vitamins, centella asiatica extract, pollen, etc. to fish collagen and elastin, enhancement of cell activity, inhibition of collagenase expression, elastin The present invention relates to an anti-aging food composition having effects such as inhibition of enzyme activity and promotion of differentiation of keratinocytes.

Aging is a process in which the structure and function of the body gradually deteriorate with age, and the susceptibility to disease and death increases rapidly and debilitates. Efforts have been made to elucidate the cause of aging. Theories that explain causation include genetic programming theory, free radical theory, and hormone-stress theory. The genetic programming theory is that our body is made up of cells, and aging occurs when these cells reach the end of their lifespan. Cells are born with a genetically limited lifespan, and changes such as cell size, number of chromosomes, and enzymes increase or decrease during life occur, and if there are no further changes, aging occurs. While this theory emphasized the factors responsible for the genetically engineered biological changes of aging, the free radical theory emphasized long-accumulating undesirable environmental factors. According to the free radical theory, aging begins by damaging the DNA repair system with a harmful environment such as unstable oxygen molecules, causing various diseases and disorders.

In terms of the phenomenon of aging, aging of the skin is representative. While the skin is in direct contact with the external environment, it plays an important protective film that protects the human body from many fatal harmful factors that try to invade the human body, for example, temperature, humidity, and ultraviolet rays. However, as we age, skin cells are damaged due to various contaminants, strong ultraviolet rays, stress, and nutritional deficiencies, and cell proliferation is not performed properly, resulting in wrinkles, loss of elasticity and keratinization of the skin. Skin aging is largely divided into intrinsic aging and extrinsic aging (Cosmetics & Toiletories, 111:31-37 (1996)). Intrinsic aging is a natural aging phenomenon that occurs due to the gradual decline in the physiological functions of the skin with age. It is clinically characterized by decreased elasticity, rough skin texture, deep wrinkles and pigmentation (Arch Dermatol., 130:87-95 (1994)). Exogenous aging refers to aging caused by extrinsic factors such as ultraviolet rays, reactive oxygen species, and stress. Due to these factors, aging of the skin proceeds rapidly, and as a result, rough wrinkles are generated on the skin surface and elasticity is significantly reduced.

Several studies have been conducted to identify the mechanism of skin aging, and various causes of skin aging have been revealed. When the skin becomes dry due to a change in temperature, a decrease in humidity, or the wind, the physiological activity of the skin as a barrier to the outside decreases and aging is accelerated. In addition, when the skin is exposed to harmful reactive oxygen species or free radicals, lipid peroxide is generated by oxidation in the body, and various proteins constituting the skin are modified. On the other hand, collagen and highly elastic elastin protein, which accounts for most of the skin's dermis (about 70-80% of the total dry weight of the skin), are major proteins produced by fibroblasts and are involved in the mechanical firmness of the skin, the bonding strength and elasticity of the tissue in the dermal layer of the skin. (Journal of the american academy of dermatology, 21:610-613 (1989)). Collagen shows a quantitative decrease as the age increases and exposure to UV rays increases. Collagen is classified according to its morphological and structural characteristics, of which the most well-known are types I, II, III and IV. Collagen types I and III are

It constitutes a component of the intercellular matrix and collagen type IV is a major component of DEJ (Dermal Epidermal Junction). DEJ is located between the epidermal and dermal layers of the skin and controls the permeation and transport of substances between the two layers, controls the differentiation of adjacent skin cells, and maintains the firmness of the skin. Collagen and elastin, which are the main structural components of the skin, are denatured or biosynthesized by natural or external stimuli, which accelerates skin aging.

In addition, since the skin has many opportunities to come into contact with various external stimuli, wrinkles are more likely to occur than other organs. Among them, facial skin is directly exposed to sunlight, dry outside air, and pollutants, so aging phenomena such as wrinkles occur earlier than skin that is not. The most characteristic change according to the aging of the skin tissue is a change in the skin matrix. As fibroblasts in the dermal layer age, the ability to generate fibers and matrix decreases. As the amount of matrix decreases overall, the thickness of the skin becomes thinner, and wrinkles are formed by lowering the elasticity of the skin. That is, as aging progresses, the elasticity of the skin deteriorates, blood circulation is impaired, and the skin barrier weakens. In addition, when the skin is exposed to ultraviolet rays, free radicals and reactive oxygen species, which are the main causes of skin cell damage, are generated. They cause DNA damage, attack the cell membrane structure and cause aging spots, and can accelerate the creation of wrinkles by attacking collagen and fibers that keep the skin moist, soft, and supple.

On the other hand, since collagen is involved in the amount of moisture in the skin, it is commonly known that eating collagen-rich foods can prevent skin aging, joint weakness, and damage to blood vessels. However, during actual intake and oral administration, it is absorbed after being broken down into amino acids such as glycine and proline through proteolysis. It is preferable to take them together.

In addition, elastin exists in connective tissue together with collagen and is a stretchable protein such as rubber elasticity, and is involved in tissue flexibility and elasticity. Therefore, the content is high in tissue where stretching is always repeated. Elastin plays an important role in skin elasticity and wrinkle prevention. When the elastin content is reduced, elastin called pseudoelastin and an amorphous substance thought to be produced from collagen are accumulated at the same time, causing the skin to lose elasticity and wrinkles to occur.

And, hyaluronic acid, which is a component of the dermal layer of the skin, is a component that fills the gaps between collagen in the dermal layer to maintain moisture and elasticity of the skin. In particular, ultraviolet rays reduce the amount of hyaluronic acid, thereby reducing the moisture content in the skin and increasing the amount of transdermal moisture loss, thereby causing damage to the skin barrier. As a result, the skin becomes rough and its elasticity is reduced, resulting in wrinkles (Baumann L, J. Pathol., 211(2):241-251, 2007). In other words, loss of moisture in the skin is a major cause of skin aging.

In addition, Centella asiatica (scientific name: Centella asiatica), which is known to be excellent for skin regeneration, is also used as a cosmetic raw material for its effects such as skin soothing and skin immunity, and is known to be effective in improving edema medically. In a recent study, we made a fermented extract containing Centella asiatica (C. asiatica) and folic acid as the main ingredients to improve cell biological efficacy in inhibiting cell aging, promoting collagen production, promoting cell regeneration and cell migration, anti-inflammatory, and keratinocytes. It has been shown to promote cell differentiation and inhibit melanogenesis.

In addition, vitamin C, which has excellent antioxidant effects, is also known for its anti-aging properties.

However, the use of various materials known for various anti-aging functions is mainly for cosmetics. In addition, when provided in the form of food, its function is often halved, and when various materials are mixed and used to enhance the anti-aging function, the anti-aging function of each material may not be exhibited on the contrary. Furthermore, even when a preferred combination of materials used to enhance the anti-aging function is known, the effect is different depending on the content ratio and other added extracts.

The researchers of the present invention, in addition to the above substances, so that the functions of collagen, elastin, and hyaluronic acid, which play an important role in anti-aging, can be more activated, by including Centella asiatica, vitamin C, royal jelly, and pollen, anti-aging as a food composition A functional ATAGE-905 collagen-elastin mixture was developed.

Republic of Korea Patent Publication No. 10-2013-0031590 Republic of Korea Patent Registration No. 10-1055153 Republic of Korea Patent Registration No. 10-1971837

An object of the present invention is to provide a highly effective anti-aging food composition.

The present invention is an anti-aging food composition comprising fish collagen, elastin, hyaluronic acid, centella leaf extract, vitamin C, royal jelly and pollen. Preferably, the composition contains 2 to 2 to elastin based on 100 parts by weight of fish collagen. 8 parts by weight, 2 to 8 parts by weight of hyaluronic acid, 70 to 180 parts by weight of Centella asiatica extract, 70 to 180 parts by weight of vitamin C, 5 to 15 parts by weight of royal jelly, and 2 to 8 parts by weight of pollen. Furthermore, the composition may further include an extract of amaranth oleifera, preferably in a ratio of 2 to 8 parts by weight of the hyacinth extract based on 100 parts by weight of fish collagen.

The anti-aging food composition of the present invention can maximize the anti-aging function through synergy between the compositions, and has the effect of being more convenient and durable by being provided in the form of food.

1 is a graph showing the antioxidant effect of the present invention.
2 is a graph showing the effect of inhibiting the expression of collagenase of the present invention.
3 is a graph showing the elastase activity inhibitory effect of the present invention.
Figure 4 is a graph showing the melanin production inhibitory effect of the present invention.
5 is a graph showing the keratinocyte differentiation promoting effect of the present invention.

The present invention is to maximize the anti-aging function by activating the functions of collagen, elastin, and hyaluronic acid. Collagen, elastin, and hyaluronic acid play important roles in anti-aging, as is already known. In particular, it is directly related to the anti-aging of the skin. In addition, Centella asiatica leaf extract is also used as a major raw material in the present invention as its anti-aging function has recently been highlighted. In addition, vitamin C is also used as the main ingredient. In addition to this, the present invention can strengthen the function of the main ingredients by further including royal jelly, pollen, and golden cherry tree. On the other hand, the present invention can provide a more convenient and more durable anti-aging function by being provided in the form of food rather than as an external preparation for skin, and shows a more optimized composition ratio for ingestion in the form of food.

The skin can be divided into the epidermis and the dermis. The dermis, which is deep inside the skin, consists of collagen, hyaluronic acid, and elastin, and the epidermis, which can be called the outer surface of the skin, consists of ceramides, etc. Each component is an essential component for healthy skin. admit. Collagen plays an important role in maintaining elasticity by tightly connecting skin cells so that they do not fall apart, and elastin acts as a spring to support collagen. It plays an important role in maintaining moisture because it is delivered to the area. Ceramide, a component of the skin barrier, prevents skin moisture from evaporating and blocks harmful substances coming in from the outside to prevent troubles. Skin balance can be maintained only when they exist in a balanced way. Collagen is like a rope in which three polypeptide molecules are twisted into a triple helix. In each polypeptide chain, glycine and proline are alternately arranged with each other, and several different amino acids are interpolated in the middle. Representatively, there are hydroxylysine and hydroxyproline derived from lysine and proline, respectively. These two play an important role in the formation of a quaternary structure in which chains are tightly connected to each other through hydrogen bonds. Collagen ingested as food is absorbed after being broken down into amino acids such as glycine and proline through proteolysis. When lysine and prolines are hydroxylated by lysyl hydroxylase and prolyl hydroxylase during collagen formation, alpha peptide cross-linking going through this process. These enzymatic reactions use vitamin C as a cofactor. If scurvy occurs due to lack of vitamin C, the hydroxylation of proline and lysine does not occur well, so the triple helix formed of three alpha peptides is loosely assembled, resulting in poor collagen structure.

The collagen used in the present invention is preferably fish collagen. This is because it is known that the absorption rate of fish collagen is better than that of normal animal collagen. And, when collagen is actually ingested, as mentioned above, since it is absorbed after being decomposed into amino acids such as glycine and proline through the proteolysis process, it is necessary to ingest a significant amount of vitamin C necessary for collagen synthesis, and the present invention also adopted this. Because vitamin C has a good antioxidant function, it is often used in cosmetics, but in the form used for skin, it is used in relatively small amounts because it has acidity. Vitamin C used as food or medicine generally plays an antioxidant role. When reactive oxygen species and reactive nitrogen species generated during menstruation are adjacent to vitamin C, they remove hydrogen from the 2nd carbon of vitamin C and remove the vitamin C. Leaving a radical in C, the radical generated in vitamin C is stabilized through a resonance structure, or is stabilized by forming dehydroascorbate after one more electron is lost. By changing these highly reactive active oxygen species and active nitrogen species radicals into stable vitamin C radical intermediates, the antioxidant action of scavenging radicals proceeds. In addition, it is known that it prevents the oxidation of cholesterol, maintains blood vessels and increases immunity by increasing interferon production.

In the present invention, since vitamin C is used as a food composition, it can be used in a relatively excessive amount. Preferably, 70 to 180 parts by weight are used based on 100 parts by weight of fish collagen. More preferably, 110 to 140 parts by weight are used, and a more optimized form is 125 parts by weight. Vitamin C in the present invention contributes to the formation and maintenance of collagen in the body. When vitamin C is insufficient, the effect of collagen formation and maintenance is lowered, and the use of more vitamin C than necessary causes rejection of intake.

In the present invention, elastin and hyaluronic acid are additionally used together with collagen. Elastin acts as a spring supporting collagen, and hyaluronic acid stores moisture in the skin and delivers it to the necessary parts, so it is preferable to use it together. It is preferable to use 2 to 8 parts by weight of elastin based on 100 parts by weight of collagen, and if it is used too little, the collagen connection becomes loose, and if used more than necessary, the balance of collagen may be disturbed. More preferably, the proportion is 4 to 6 parts by weight, and the optimized proportion is 5 parts by weight. It is also preferable to use hyaluronic acid in an amount of 2 to 8 parts by weight based on 100 parts by weight of collagen. Because hyaluronic acid functions between collagen and elastin, if it is used too little, it is difficult to show its proper function. More preferably, the proportion is 4 to 6 parts by weight, and the optimized proportion is 5 parts by weight.

Another of the main raw materials used in the present invention is Centella asiatica leaf extract. According to prior papers, there has been mentioned about the combined use of Centella asiatica extract and folic acid, and it is reported that it has a significant effect on anti-aging. Centella asiatica extract is used in an amount of 70 to 180 parts by weight based on 100 parts by weight of fish collagen. More preferably, 110 to 140 parts by weight are used, and a more optimized form is 125 parts by weight. In the present invention, Centella asiatica leaf extract is responsible for the main mechanism of anti-aging together with fish collagen, and when it is used too little, it is insufficient to achieve the desired purpose. does not need to be used as In the present invention, it is preferable to use royal jelly and pollen (pollen) together with the Centella asiatica leaf extract, and serves to further strengthen and stabilize the function of the Centella asiatica leaf extract. Royal jelly and pollen contain various vitamin components, rare minerals, and antibacterial components. In the case of royal jelly, it is preferable to use 5 to 15 parts by weight based on 100 parts by weight of collagen. If you use too little, the desired effect will be insignificant, and if you use it more than necessary, it may act as an allergen. More preferably, the proportion is 8 to 12 parts by weight, and the optimized proportion is 10 parts by weight. In the case of pollen, it is preferable to use 2 to 8 parts by weight based on 100 parts by weight of collagen. Like royal jelly, pollen, if used too little, may not function properly, and if used more than necessary, it may act as an allergen. More preferably, the proportion is 4 to 6 parts by weight, and the optimized proportion is 5 parts by weight.

On the other hand, in the present invention, it is possible to additionally use an extract of geranium oleracea. It is judged that Geumaeja extract enhances the functional aspects of collagen or Centella asiatica extract. Geumaeja is known for its excellent antibacterial action. In Donguibogam, it is recorded that it cures diarrhea and excessive urine output, prevents semen from coming out, and stops oil wells and mongseol, but it seems to prevent the loss of body fluid in general. It is judged to be helpful in maintaining collagen in the present invention as well, and it is judged that the synergistic effect of the combination of collagen, centella asiatica, and golden cherry tree is exhibited. In the case of golden cherry, it is preferable to use 2 to 8 parts by weight based on 100 parts by weight of collagen. If you use too little, it is difficult to function properly, and if you use it for more than necessary, there may be side effects such as constipation if you take it for a long time. More preferably, the proportion is 4 to 6 parts by weight, and the optimized proportion is 5 parts by weight.

In the present invention, fish collagen, Centella asiatica leaf extract, and vitamin C are mainly used, and hyaluronic acid, elastin, pollen, and golden cherry, which are used as auxiliary or functional enhancing substances, are used in relatively small amounts, and the range of preferred usage is similar. Hereinafter, the present invention will be described in more detail through Examples and Test Examples. However, these examples and the like are used for the purpose of illustration to help the understanding of the present invention, the scope and scope of the present invention is not limited thereto.

<Preparation of ingredients>

Fish collagen, vitamin C, royal jelly, hyaluronic acid, elastin, and pollen use ready-made raw materials that meet the standards and specifications of the Food and Additives Code. Centella asiatica leaf extract and golden apricot extract are prepared in the following way.

- Centella asiatica leaf extract

3 kg of Centella asiatica leaves purchased from the Seoul Pharmacy Store were dried and pulverized in the shade and at room temperature for 5 days. The pulverized Centella asiatica leaves were immersed in 30 L of 95% ethanol and extracted at 50° C. for 24 hours. This was filtered through a filter paper, dried and concentrated under reduced pressure at 45° C. to obtain 480 g of a total extract.

- Golden primrose extract

The extract was obtained in the same way using 3 kg of Geum aengja purchased from the Seoul Pharmacy Store, to obtain a total extract of 490 g.

<Example 1>

Based on 100 parts by weight of fish collagen, the composition was composed of 5 parts by weight of elastin, 5 parts by weight of hyaluronic acid, 125 parts by weight of Centella asiatica extract, and 125 parts by weight of vitamin C.

<Example 2>

In Example 1, 10 parts by weight of royal jelly was added to constitute a composition.

<Example 3>

5 parts by weight of pollen was added to Example 1 to constitute a composition.

<Example 4>

In Example 1, 10 parts by weight of royal jelly and 5 parts by weight of pollen were added to constitute a composition.

<Example 5>

To Example 1, 5 parts by weight of an extract of Geumaengjae was added to constitute a composition.

<Example 6>

Example 4 was added to 5 parts by weight of the extract of Euphorbia oleracea to form a composition.

<Test Example 1> Antioxidant effect

In order to examine the antioxidant effect of the examples of the present invention, a free radical scavenging activity experiment was performed. This was performed as a method of evaluating antioxidant activity by comparing and measuring the DPPH oxidation inhibitory efficacy through the change in absorbance caused by the reduction of the free radical DPPH (1,1-diphenyl-2-picryl hydrazyl). As a positive control, a widely used synthetic antioxidant, Trolox, was used.

190 μl of 100 μM (in ethanol) DPPH solution and 10 μl of each of the examples and positive control of the present invention were added to prepare a reaction solution, and after reacting at 37° C. for 30 minutes, absorbance was measured at 540 nm.

The IC50 of Trolox was 47 ppm, and the IC50 of Example 1 was 107, showing a low antioxidant effect when compared with Trolox. In the case of Examples 2 and 3, the antioxidant effect was improved by showing 82 and 83, respectively. In the case of Example 4, 53 showed an antioxidant effect comparable to Trolox. Example 5 showed some improvement effect. Example 6 showed an excellent antioxidant effect. This is shown in FIG. 1 .

<Test Example 2> Collagenase expression inhibitory effect

Collagenase expression inhibitory ability of the above examples was measured in comparison with EGCG. As an antioxidant, EGCG is known to have the function of preventing skin aging by regenerating epidermal cells of the skin.

Human fibroblasts were placed in a 96-well plate containing DMEM medium containing 2.5% urea serum at a volume of 5,000 cells per well and cultured until 80% growth. Examples and EGCG were treated at a concentration of 20 μg/ml for 24 hours, and then the cell culture solution was collected. Using a commercially available collagenase measuring instrument, the collagenase production level of the collected cell culture medium was measured. First, the collected cell culture solution was put into a 96-well plate coated with collagenase antibody uniformly, and the antigen-antibody reaction was performed in a thermostat for 3 hours. Thereafter, a secondary collagen antibody bound to a chromophore was placed in a 96-well plate to induce color development at room temperature for 15 minutes, and then 1M sulfuric acid was added to stop the color reaction. The color is yellow, and the degree of yellowness is different depending on the degree of reaction progress. The absorbance was measured at 405 nm, and the expression level was investigated by dividing the absorbance of the treated group by the absorbance of the untreated group. It is believed that the lower the expression level of collagenase, the higher the ability to inhibit the expression of collagenase, the less decomposition of collagen in the skin, the decrease in skin elasticity is inhibited, and the amount of wrinkles generated is reduced.

When the expression level of the untreated group was 100, EGCG was 75%, and Example 1 partially suppressed the expression of collagenase as 85%. In the case of Examples 2 and 3, the inhibitory power was improved as 78 and 79, and in the case of Example 4, an effect similar to that of EGCG was shown. Example 5 also showed a better effect than Example 1, and Example 6 had the best effect. This is shown in FIG. 2 .

<Test Example 3- Elastase activity inhibitory effect>

The elastase activity inhibitory ability of the Examples was measured in comparison with EGCG. Experiments were conducted by applying the method of Cannell et al. (Cannell RJP, Kellan SJ, Owsianks AM, Walker JM. Results of a large scale screen of microalgae for the production of protease inhibitors. Planta Med. 1988;54(1):10 -14). Using N-succinyl-(L-Ala)3-p-nitroanilide as a substrate, the amount of pnitroanilide produced from the substrate was measured at 445 nm at 37°C for 20 minutes. That is, each test solution is prepared to a constant concentration, taken 0.5 ml each, into a test tube, 0.5 ml of a porcine pancreas elastase (2.5 U/ml) solution dissolved in 50 mM tris-HCl buffer (pH 8.6) is added, and then 50 mM tris- as a substrate. N-succinyl-(L-Ala)3-p-nitroanilide (0.5 mg/ml) dissolved in HCl buffer (pH 8.6) was added, followed by reaction for 20 minutes. Elastase inhibitory activity was calculated as (1 - absorbance of treated group / absorbance of untreated group) * 100.

When the inhibition rate of the untreated group was 0%, EGCG was 21%, Example 1 was 21%, which was similar to EGCG. Examples 2 and 3 showed 35 and 35, respectively, and Example 4 was quite good with 52, respectively. The case of Example 5 was also improved to some extent to 33, and Example 6 showed very good properties. These are shown in FIG. 3 .

<Test Example 4 - Melanin production inhibitory effect>

The melanin production inhibitory effect of Examples was measured in comparison with hydroquinone, a known whitening material. C57BL/6 mouse-derived mouse pigment cells (Mel-Ab cells) (Dooley, TP et al, Skin pharmacol, 7, pp 188-200) in DMEM with 10% placental bovine serum, 100 nM 12-O-tetradecanoyl It was cultured in a medium supplemented with tetradecanoylphorbol-13-acetate and 1 nM cholera toxin at 37° C. and 5% CO 2 conditions. The cultured Mel-Ab cells were removed with 0.25% trypsin-EDTA, and the cells were cultured at a concentration of 100 cells/well (cells/well) in a 24-well plate, and then each test substance was added and cultured for 3 consecutive days from the second day. did. Hydroquinone and Examples were used as test substances at a concentration of 10 μg/ml. Then, the culture medium was removed, washed with PBS, cells were dissolved with 1N sodium hydroxide, and absorbance was measured at 400 nm, and then (1 - absorbance of treated group / absorbance of untreated group) * 100 was obtained as follows.

When the inhibition rate of melanogenesis in the untreated group was 100, the inhibition rate of hydroquinone was 43%, and Example 1 showed 30%, showing some effect. In the case of Examples 2 and 3, the effects were slightly improved to 37 and 35, respectively, and in the case of Example 4, the 45 showed a very good effect, confirming some synergy. In the case of Example 5, 33 showed an effect to some extent, and Example 6 was 48, which confirmed additional synergy. This is shown in FIG. 4 .

<Test Example 5 - Keratinocyte differentiation promoting effect>

In order to examine the differentiation-promoting effect of keratinocytes, the amount of CE (Cornified Envelop) generated during differentiation of keratinocytes was measured using absorbance as follows. First, human keratinocytes separated from the epidermis of newborns and primary cultured are placed in a culture flask and attached to the bottom, and then treated with the material of Example at a concentration of 10 ppm in the culture medium. When the cells grow to about 80% of the floor area until incubated for 5 days. At this time, the low calcium (0.03mM) treatment group and the high calcium (1.2mM) treatment group were used as a negative control and a positive control, respectively. Then, the cultured cells are harvested, washed with PBS (Phophate buffered saline), and 10 mM concentration of tris-hydrochloric acid buffer (Tris-HCl, pH 7.4) was added to 1 ml, sonication, boiling, and centrifugation were performed, and the precipitate was suspended again in 1 ml of PBS, and absorbance at 340 nm was measured. Separately, a portion of the solution after sonication was taken to measure the protein content, and was used as a standard when evaluating the degree of cell differentiation.

When the negative control group had a differentiation potency of 100, the positive control group showed 198, Example 1 showed 135, Example 2 showed 151, and Example 3 showed 153, and Example 4 showed 176 because of a synergistic effect. Example 5 showed an effect of 148 to some extent, and Example 6 had a synergistic effect of 181, which was very good. This is shown in FIG. 5 .

<Sensory evaluation>

For 30 women in their 50s classified as dry skin, 10 each were given to ingest the food compositions of Examples 1, 4, and 6 every morning and evening. After 10 days, a survey was conducted on skin elasticity and moisture level. Overall, a response was received that there was an improvement effect, and the satisfaction of Examples 4 and 6 was relatively high.

<Change in Addition Ratio>

The content ratio of Centella asiatica leaf extract, vitamin C, elastin, hyaluronic acid, pollen, and Pomegranate extract was variously adjusted based on the above examples. out of , the effect was somewhat lowered.

From the above results, it was possible to confirm the anti-aging effect of the composition of the present invention.

Claims (8)

An anti-aging food composition comprising fish collagen, elastin, hyaluronic acid, centella asiatica extract, vitamin C and royal jelly. An anti-aging food composition comprising fish collagen, elastin, hyaluronic acid, centella asiatica extract, vitamin C and pollen. An anti-aging food composition comprising fish collagen, elastin, hyaluronic acid, centella asiatica extract, vitamin C, royal jelly and pollen. 4. The method according to any one of claims 1 to 3,
The composition comprises 2 to 8 parts by weight of elastin, 2 to 8 parts by weight of hyaluronic acid, 70 to 180 parts by weight of Centella asiatica extract, 70 to 180 parts by weight of vitamin C, 5 to 15 parts by weight of royal jelly based on 100 parts by weight of fish collagen, An anti-aging food composition, characterized in that the proportion of pollen is 2 to 8 parts by weight. 5. The method of claim 4,
The composition is characterized in that the ratio is 5 parts by weight of elastin, 5 parts by weight of hyaluronic acid, 125 parts by weight of Centella asiatica extract, 125 parts by weight of vitamin C, 10 parts by weight of royal jelly, and 5 parts by weight of pollen based on 100 parts by weight of fish collagen. Anti-aging food composition. 4. The method of claim 3,
The composition is an anti-aging food composition, characterized in that it further comprises an extract of Astragalus. 7. The method of claim 6,
The composition comprises 2 to 8 parts by weight of elastin, 2 to 8 parts by weight of hyaluronic acid, 70 to 180 parts by weight of Centella asiatica extract, 70 to 180 parts by weight of vitamin C, 5 to 15 parts by weight of royal jelly based on 100 parts by weight of fish collagen. , 2 to 8 parts by weight of pollen, Anti-aging food composition, characterized in that the ratio of 2 to 8 parts by weight of the ginseng extract. 8. The method of claim 7,
The composition contains 5 parts by weight of elastin, 5 parts by weight of hyaluronic acid, 125 parts by weight of Centella asiatica extract, 125 parts by weight of vitamin C, 10 parts by weight of royal jelly, 5 parts by weight of pollen, and 5 parts by weight of golden cherry tree extract based on 100 parts by weight of fish collagen. An anti-aging food composition, characterized in that the ratio is negative.

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