KR20210105234A - The Development of Cosmeceutical Products for Whitening and Anti-wrinkle Cosmetic Agent by using bracken(Pteridium aquilinum) bio -conversion substance - Google Patents

Abstract

The present invention relates to a composition comprising, as an active ingredient, fermented Pteridium extract having skin whitening and wrinkle alleviation effects, wherein the fermented Pteridium extract of the present invention can be usefully used as a raw material for cosmeceutical products.

Description

The Development of Cosmeceutical Products for Whitening and Anti-wrinkle Cosmetic Agent by using bracken(Pteridium aquilinum) bio -conversion substance}

The present invention relates to the use of a fermented bracken extract having skin whitening and wrinkle-improving effects using bracken bioconverting material as a raw material for cosmeceutical products.

It is a widely known fact that fermented foods that have been fermented for a long time are good for the body. However, when using fermented substances obtained from microorganisms as cosmetic ingredients for skin application, the amount of fermented substances that contain a lot of useful ingredients for the skin is very small. In addition to the technology of extracting the fermented material, there was a limitation in that it required a high level of technology, such as removing the odor emitted from the microorganism itself and purely separating only the fermented material. Nevertheless, in recent years, the domestic cosmetic industry has succeeded in developing a technology that can extract fermented substances in large quantities, and there have been achievements such as converting substances extracted from soybeans into usable ingredients through microbial fermentation technology and mass-producing them. It has been shown that these substances are highly absorbed from the skin and enhance elasticity by promoting collagen production in the skin tissue.

Skin aging can be divided into intrinsic, chronological aging and phtoaging [Gilchrest BA: J. Am. Acad. Dermatol., 21, 610-613 (1989)]. Intrinsic aging is an aging phenomenon that is naturally induced by the deterioration of physiological functions of the living body with increasing age [Braverman IM et al.: J. Invest. Dermatol., 78, 434-443 (1982)]. Photoaging refers to a change in the appearance or function of the skin due to repeated exposure to light [Ridder GM et al.: J. Am. Acad. Dermatol., 25, 751-760 (1991)]. In addition, skin aging can be caused by the activation of reactive oxygen species according to ultraviolet rays, stress, disease states, environmental factors, wounds, and aging. It damages tissues and promotes adult diseases and aging. More specifically, lipids, proteins, polysaccharides, nucleic acids, etc., which are major constituents of the skin, are oxidized to destroy skin cells and tissues, and eventually skin aging occurs. In particular, the oxidation of proteins causes severe hyper-inflammatory reactions due to the cutting of the connective tissues of the skin, such as collagen, hyaluronic acid, elastin, proteoglycan, and fibronectin, and interferes with the elasticity of the skin. It leads to mutation, the induction of cancer, and the deterioration of immune function.

Therefore, it is necessary to protect the cell membrane by eliminating reactive oxygen species generated during the body's metabolic process, UV irradiation, and inflammatory reaction, and to regenerate damaged cells through active metabolism to proliferate the cells. can recover and maintain healthy skin. In aging, not only reactive oxygen species but also an enzyme called Matrixmetallopretease (MMP) are involved. In vivo, the synthesis and degradation of extracellular matrix, such as collagen, is properly regulated, but as aging progresses, the synthesis decreases, and the matrix, which is an enzyme that breaks down collagen As the expression of metalloproteinase (MMP) is promoted, the elasticity of the skin is reduced and wrinkles are formed. In addition, these decomposing enzymes are activated by exposure to UV rays. Therefore, there is a need for the development of substances capable of regulating or inhibiting the activity of MMP expression induced in cells by ultraviolet rays. Most of the raw materials used as cosmetic materials so far simply inhibit the MMP enzyme activity.

Next is skin changes due to pigmentation. The skin normally produces melanin to protect it from the ultraviolet rays of the sun. Melanin is produced in melanocytes, a type of skin cell, and is distributed on the skin surface to show a natural screening function to block ultraviolet rays harmful to the human body. There is an enzyme called tyrosinase that is synthesized from melanocytes in the mechanism of melanin production. Tyrosinase uses an amino acid called tyrosine as a substrate to produce dopaquinone through dopa (DOPA), and melanin, a black pigment, is produced from dopaquinone through several stages of oxidative condensation. Already ascorbic acid (JP Hei 4-9320), hydroquinone (JP Hei 6-192062), kojic acid (JP Hei 56-7710), arbutin (JP Hei 4-9315) and some extracts of , are used as whitening cosmetics because they show tyrosinase inhibitory activity, but their use is limited due to poor stability in the prescription system, decomposing and coloring, the occurrence of off-flavor, ambiguity of efficacy, effectiveness at the biological level, and safety issues, etc. It is becoming. And, although the inhibitory effect of tyrosinase has been proven, the effect is low in experiments similar to the actual biological level.

Next, the skin disease refers to abnormal findings appearing on all skin including the hair, nails, and toenails of mammals including humans, and atopic dermatitis in people with atopic allergy is a representative skin disease. Although the cause of atopic dermatitis has not yet been clearly identified, about 70% of patients with atopic dermatitis have a history of atopic dermatitis, and it occurs well in people with an allergic constitution. It is understood as one of factors or immune diseases. As a characteristic of this disease, most of the skin is scratched by severe itching in the early stage, and it may be further aggravated depending on the secondary infection and, in particular, the patient's mental stability and emotional state. Atopic dermatitis affects a fairly large number of people, with 0.5 to 1% of the total population and 5 to 10% of children suffering from it. Symptoms usually appear within 2 to 6 months of age, especially in infants under 1 year of age, and 85% appear within 5 years of age. Atopic dermatitis occurs mainly in infancy, but recently, the number of patients after puberty is increasing, and the history is also very long. For the treatment of atopic dermatitis, drugs such as adrenocortical hormones, which can be expected to have high pharmacological effects, are used. However, the side effects of such corticosteroids are a problem, and the patient is exposed to the risk of side effects during the treatment period. Moreover, it is well known that rebound (dramatic worsening of the condition of the affected area that occurs after discontinuation of use of the drug) occurs due to discontinuation of use of corticosteroids. As a countermeasure against such side effects, non-steroidal anti-inflammatory drugs or antihistamines that do not contain hormones such as adrenocortical hormones are used, but a complete cure is difficult. Therefore, it is very urgent to develop a therapeutic agent or to alleviate atopic dermatitis without side effects.

Recently, much attention has been paid to functional cosmetics having functions such as antioxidant, wrinkle relief, whitening, and itch relief obtained from natural extracts in order to reduce skin irritation caused by various chemicals. Natural ingredients have fewer side effects on the skin, and as consumers' response to cosmetics using natural ingredients increases, the value of development as a cosmetic raw material is increasing. For example, US Patent Publication No. 5,972,341 describes the anti-wrinkle effect of a plant of the genus Commiphora, in particular, an extract of Commiphora mukul. Japanese Patent Application Laid-Open No. Hei 9-672662 describes seaweed extracts having hyaluronidase inhibitory effects. Japanese Patent Laid-Open No. 10-85905 describes the skin texture improvement effect of fucoidan extracted from Wakame. Japanese Patent Laid-Open No. 2-245087 describes the antioxidant effect of an extract of the genus Sargassum. Japanese Patent Laid-Open No. Hei 3-294384 describes an antioxidant composition extracted from the genus Hizikia. Japanese Patent Laid-Open No. 7-10769 describes an extract of Phaeophyceae having an astringent effect. Korean Patent No. 0431076 discloses a cosmetic composition for improving the healing of atopic skin comprising extracts of baekgeum, perilla, echinacea and fermented soybean, and Korean Patent No. 0511494 discloses treatment of atopic dermatitis including sauerkraut, shiitake, and illite. Korean Patent Publication No. 2004-65126 discloses an effect on the healing and prevention of atopic skin containing oriental plant extracts obtained from natural reishi mushroom, Eugeunpi, licorice, baekbongryeong, baekjima and baeknyeoncho. Disclosed is an oriental cosmetic composition having In addition, Korean Patent Laid-Open No. 2004-11584 discloses a composition excellent for the treatment of atopic skin diseases, which is composed of purified and mixed substances of natural ingredients such as licorice, chanterelle, safflower, and pork (pork fat).

Next, androgenetic alopecia is male hormone-dependent and has a direct relationship with the amount of male hormone, and thus, many studies have been recently reported for the prevention and treatment of hair loss through inhibition of male hormone activity. On the other hand, when the function of the sebaceous glands becomes active due to the increase in the secretion of male hormones, comedones formed as excess sebum is stagnant in the hair follicles due to hyperkeratinization of the hair follicle wall is the initial stage of acne. When explaining the mechanism of hair loss and acne caused by male hormones, 5-alpha-reductase (5-α-Reductase) is one of male hormones present in male hormone-responsive tissues such as sebaceous glands, hair follicles, prostate, and epididymis. , an enzyme involved in metabolizing testosterone to dihydrotestosterone, and the conversion requires NADPH. In addition, testosterone (testosterone) is involved in male sexual drive, skeletal muscle increase, male external genitalia, scrotal growth, spermatogenesis, etc., and dihydrotestosterone (dihydrotestosterone) is involved in the corresponding tissues such as acne, sebum increase, hair loss and prostate enlargement. In particular, excessive secretion of male hormone after puberty causes acne and hair loss. To prevent excessive production of dihydrotestosterone, the active form of male hormone produced by 5-alpha-reductase, Researches to develop anti-hair loss and anti-acne agents using enzyme inhibitors are being actively conducted.

In order to solve these skin problems, cosmetics using natural products have been developed in recent years to reduce skin irritation caused by various chemicals. Natural ingredients have fewer side effects on the skin, and as consumers' response to cosmetics using natural ingredients increases, the value of development as a cosmetic raw material is increasing.

An object of the present invention relates to the effect of enhancing the skin absorption rate of a fermented extract of bracken, and an effective course for wrinkle improvement, whitening effect, and prevention of skin aging, which contains bracken extract whose skin absorption rate is improved by fermenting bracken to low molecular weight. To provide a mechanical product composition.

In order to achieve the above object, the present invention provides a cosmetic composition comprising a fermented bracken extract having an effect of enhancing skin absorption as an active ingredient and a method for preparing the same.

The fermented bracken extract of the present invention can be obtained by adding ground bracken to purified water, heat sterilizing, and inoculating lactic acid bacteria in the bracken extract made by filtration, inoculating the anaerobic culture medium, sterilizing and filtering.

The present invention provides a composition comprising the fermented bracken extract obtained by the above manufacturing process as an active ingredient.

The lactic acid bacteria fermented bracken extract obtained by the above method has a superior skin absorption rate than existing substances, so it can be used as an effective cosmetic raw material for wrinkle improvement, whitening effect, and skin aging prevention.

For the above purpose, the present invention puts pulverized bracken in purified water, heat sterilizes, and inoculates lactic acid bacteria into a bracken extract made by filtration, anaerobically cultured for 5 to 120 hours, and then sterilized and filtered. It provides a cosmeceutical product composition comprising as an ingredient.

In the present invention, the term "lactic acid bacteria" is a bacterium that decomposes carbohydrates such as glucose or lactose to produce organic acids such as lactic acid or acetic acid, Lactobacillus, Streptococcus, Bifidobacterium, Leuconostok, Pediococcus or Lactobacillus It refers to microorganisms belonging to the genus of 6 lactic acid bacteria in the genus Caucus. In the present invention, any one or more species belonging to the genus of lactic acid bacteria can be selected or used in combination. Most preferably, it is Lactobacillus casei PM1 (Accession No.: KCCM 10766P).

Lactobacillus casei PM1 (Accession No.: KCCM 10766P) strain is a gram-positive, spore-free, rod-shaped bacterium. ) The colonies produced by smearing each 100 μl of the 10 ^-4 -10 ^-5 dilution on agar were first selected, and the second selection was performed on MRS (DIFCO) agar medium containing 0.5% CaCO3. Teeth picking was performed and cultured at 37° C. for 48 hours, and when a transparent ring was formed around a colony, it was determined that the first selected microorganism produced an acid, and the strain was selected. The strains selected in the second stage were subjected to Gram staining as a tertiary morphological observation and then observed under a microscope to select only Gram-positive and rod-type microorganisms. As for the microorganisms formed in this way, only negative bacteria were selected in the catalase reaction using hydrogen peroxide. The culture condition of Lactobacillus casei PM1 (Accession No.: KCCM 10766P) is 35-37°C, and although it has facultative anaerobic characteristics, it is considered suitable for the production of cosmetic raw materials when cultured under anaerobic conditions. The strain of the present invention was deposited with the accession number KCCM 10766P at the Korea Microorganism Conservation Center (KCCM) on July 25, 2006.

The cosmeceutical product composition comprising the fermented bracken extract according to the present invention may include a cosmetically acceptable carrier. In the present invention, the term "cosmetically acceptable carrier" refers to one or more cosmetically acceptable solid or liquid fillers, diluents, extenders, and the like. As used herein, the term "compatibility" means that the components of the composition of the present invention can be mixed with the basic active agent of the present invention and mixed with each other in such a way that there is no interaction that substantially reduces the efficacy of the composition under normal use. means there is The type of carrier used in the present invention depends on the type of product required. The topical compositions useful in the present invention can be formulated into a variety of product types. These include, but are not limited to, lotions, gels, sprays, ointments and mousses. These product types can include several types of carriers including, but not limited to, solutions, aerosols and gels. In the present invention, "topical application (application)" means applying or painting directly on the external skin.

In the present invention, "skin lightening" refers to overall whitening of one or more basic skin tones, i.e. hyperpigmentation, including age spots, melanoma, liver spots, freckles, post-inflammatory hyperpigmentation or sun-induced pigmentation defects. It means to reduce the melanin in the skin, including the whitening of the disorder.

A second aspect of the present invention comprises the steps of preparing a bracken extract made by putting ground bracken in purified water, heat sterilizing, and filtering; Inoculating the Lactobacillus casei PM1 (Accession No.: KCCM 10766P) strain into the bracken extract and culturing anaerobically for 5 to 120 hours; and sterilizing and filtering the lactic acid bacteria fermented extract.

A third aspect of the present invention is to prepare a bracken extract made by putting ground bracken in purified water, heat sterilization, and filtering; Lactobacillus casei PM1 (accession number: KCCM 10766P) strain is inoculated into the bracken extract and anaerobic for 5 to 120 hours culturing; and sterilizing and filtering the lactic acid bacteria fermented extract.

A fourth aspect of the present invention comprises the steps of preparing a bracken extract made by putting ground bracken in purified water, sterilizing heat and filtering; Inoculating the Lactobacillus casei PM1 (Accession No.: KCCM 10766P) strain into the bracken extract and culturing anaerobically for 5 to 120 hours; And it provides a method for producing an anti-aging cosmeceutical product composition comprising the step of sterilizing and filtering the lactic acid bacteria fermented extract.

Hereinafter, the present invention will be described in detail by way of Examples. However, the following examples are merely illustrative of the present invention, and the content of the present invention is not limited thereto.

Example 1. Preparation of bracken extract

Put 90g of a mass of appropriately crushed Russian bracken (wonyong) with a grinder twice in 300ml of purified water, and then sterilize at 121℃ for 15 minutes. The filtered filtrate was used as a bracken extract.

Example 2. Lactic acid bacteria seed culture

1. Put modified MRS (0.05% L-cystein, free beef extract) into a 10 ml cap tube, inoculate Lactobacillus casei PM1 (Accession No.: KCCM 10766P) after sterilization, and incubate anaerobically at 37°C for 12 hours.

2. After inoculating the culture solution into 100 ml modified free beef extract (MRS), it is anaerobically cultured at 37° C. for 12 hours.

Example 3. Fermentation of bracken extract

1. After mixing 10 ml of the extract according to Example 1, 8.5 g NaCl, and 0.5 g L-cysteine in 890 ml of purified water in a fermenter (BIO STAT® B.braun. Germany), the pH was corrected to 65, pH electrode and DO electrode Insert and sterilize at 121°C for 15 minutes.

2. After sterilization, cool the fermenter slowly to 37℃, and then inject mixed gas (5% H2, 10% CO2, N2 filled with residual gas) to replace the inside of the fermenter with mixed gas. At this time, the DO value should be "0".

3. The culture solution according to Example 2 was inoculated into the fermenter and anaerobically cultured at 80 rpm at 37° C. for 24 hours.

4. After fermentation, sterilize at 121℃ for 15 minutes.

5. Filter by 0.45um ultrafiltration.

Claims (8)

A cosmeceutical containing a fermented bracken extract obtained by sterilizing and filtering as an active ingredient after inoculating a strain of lactic acid bacteria into a bracken extract made by heating and sterilizing purified water and filtration, anaerobically cultured for 5 to 120 hours. product composition. Fermented bracken obtained by inoculating a strain of Lactobacillus casei PM1 (Accession No.: KCCM 10766P) strain into a bracken extract made by heating and sterilizing purified water, sterilizing the ground bracken in purified water, culturing anaerobically for 5 to 120 hours, and then sterilizing and filtering the fermented bracken. A cosmeceutical product composition comprising an extract as an active ingredient. Preparing a bracken extract made by adding ground bracken to purified water, sterilizing by heat, and filtering;
Inoculating the lactic acid bacteria in the bracken extract and anaerobic culture for 5 to 120 hours; and
Method for producing a whitening cosmeceutical product composition comprising the step of sterilizing and filtering the lactic acid bacteria fermented extract. Preparing a bracken extract made by adding ground bracken to purified water, sterilizing by heat, and filtering;
Inoculating the Lactobacillus casei PM1 (Accession No.: KCCM 10766P) strain into the bracken extract and culturing anaerobically for 5 to 120 hours; and
Method for producing a whitening cosmeceutical product composition comprising the step of sterilizing and filtering the lactic acid bacteria fermented extract. Preparing a bracken extract made by adding ground bracken to purified water, sterilizing by heat, and filtering;
Inoculating the lactic acid bacteria in the bracken extract and anaerobic culture for 5 to 120 hours; and
A method of manufacturing a wrinkle-improving cosmeceutical product composition comprising the step of sterilizing and filtering the lactic acid bacteria fermented extract. Preparing a bracken extract made by adding ground bracken to purified water, sterilizing by heat, and filtering;
Inoculating the Lactobacillus casei PM1 (Accession No.: KCCM 10766P) strain into the bracken extract and culturing anaerobically for 5 to 120 hours; and
A method of manufacturing a wrinkle-improving cosmeceutical product composition comprising the step of sterilizing and filtering the lactic acid bacteria fermented extract. Preparing a bracken extract made by adding ground bracken to purified water, sterilizing by heat, and filtering;
Inoculating the lactic acid bacteria in the bracken extract and anaerobic culture for 5 to 120 hours; and
Method for producing an anti-aging cosmeceutical product composition comprising the step of sterilizing and filtering the lactic acid bacteria fermented extract. Preparing a bracken extract made by adding ground bracken to purified water, sterilizing by heat, and filtering;
Inoculating the Lactobacillus casei PM1 (Accession No.: KCCM 10766P) strain into the bracken extract and culturing anaerobically for 5 to 120 hours; and
Method for producing an anti-aging cosmeceutical product composition comprising the step of sterilizing and filtering the lactic acid bacteria fermented extract.