KR20210077096A - Skin wrinkle improvement composition comprising natural oriental medicine extract as an active ingredient - Google Patents

Abstract

The present invention relates to a composition for reducing skin wrinkles comprising an herbal medicine extract as an active ingredient. Any one or more extracts selected from the group consisting of Vitis amurensis Ruprecht and Actinidia arguta according to the present invention have excellent skin wrinkle reduction effects, and can be applied as a cosmetic composition or a quasi-drug composition for reducing skin wrinkles.

Description

Skin wrinkle improvement composition comprising natural oriental medicine extract as an active ingredient}

The present invention relates to a composition for improving skin wrinkles comprising, as an active ingredient, any one or more extracts or fractions thereof selected from the group consisting of herbal medicines, specifically Vitis amurensis Ruprecht and Actinidia arguta.

The skin consists of the epidermis, dermis and subcutaneous tissue, and acts as a barrier to protect internal organs from environmental toxins. The epidermis is the outermost layer of keratinocytes that secrete keratin proteins and lipids to form an extracellular matrix (ECM), melanocytes that produce pigments, and Langerhans cells that play an antigen presentation role. cells), etc. The dermis is the skin layer under the epidermis and is composed of connective tissue that provides tension and elasticity to the skin through the ECM composed of collagen fibrils, microfibrils, and elastic fibers. The subcutaneous tissue is composed of fibroblasts that produce ECM, macrophages that remove pathogens, and adipocytes that preserve body fat.

Skin aging is caused by intrinsic factors such as genetic mutations, cellular metabolism and hormonal changes, and extrinsic factors such as chemicals, toxins, pollutants, and ultraviolet (UV) and radiation. The number of fibroblasts is reduced by the above factors, collagen and elastin are reduced or decomposed, and eventually the ECM component is atrophied and aging proceeds. In addition, the above-mentioned degradation of collagen and elastin is intensified by the activity of matrix metalloproteinases (MMPs) secreted from keratinocytes and fibroblasts, etc., and the decrease in collagen and elastin levels mainly reduces the clinical symptoms of aging skin such as wrinkles and sagging. known to cause

Accordingly, as a result of research on cosmetics for preventing aging or wrinkles of the skin, a wrinkle-improving cosmetic composition containing an extract of red rhododendron root (Korean Patent Publication No. 2015-0143375), elderberry and euari extract as active ingredients. Various compositions such as a cosmetic composition for improving wrinkles (Korean Patent Publication No. 2015-0009266) have been developed.

However, skin wrinkle-improving cosmetics developed so far do not obtain the effect as expected by users, and therefore, there is still a need for development and research on a cosmetic composition exhibiting an excellent skin wrinkle improvement or anti-aging effect.

Under this background, the present inventors completed the present invention by confirming that the extract of Vitis amurensis Ruprecht or Actinidia arguta has an effect on skin wrinkle improvement while studying the skin wrinkle improvement effect of various herbal medicines. did.

One object of the present invention is to provide a cosmetic composition for improving skin wrinkles comprising, as an active ingredient, any one or more extracts or fractions thereof selected from the group consisting of Vitis amurensis Ruprecht and Actinidia arguta. .

Another object of the present invention is to provide a quasi-drug composition for skin wrinkle improvement comprising any one or more extracts or fractions thereof selected from the group consisting of meurugeun and mihu, etc. as an active ingredient.

Another object of the present invention is whitening, skin aging prevention, including the step of administering a composition comprising as an active ingredient any one or more extracts or fractions thereof selected from the group consisting of meurugeun and mihu, etc. Or to provide a method for improving skin wrinkles.

This will be described in detail as follows. Meanwhile, each description and embodiment disclosed in the present invention may be applied to each other description and embodiment. That is, all combinations of the various elements disclosed herein fall within the scope of the present invention. In addition, it cannot be considered that the scope of the present invention is limited by the specific descriptions described below.

In order to achieve the above object, one aspect of the present invention is a skin wrinkle improvement comprising any one or more extracts or fractions thereof selected from the group consisting of Vitis amurensis Ruprecht and Actinidia arguta as an active ingredient. A cosmetic composition is provided.

As used herein, the term, “ Vitis amurensis Ruprecht” is a deciduous vine of the Grape family, also called sanpo-do and Wangmeoru. It grows wild in forests between the foothills of mountains or valleys at an altitude of 100 to 1,650 meters above sea level in Japan, China and Korea, and the fruits are known to be raw or planted as garden trees or fruit trees while making alcohol.

In the present invention, the term " Mihudeung ( Actinidia arguta )" is a creeping deciduous shrub of the Actinidae family, also called Actinidia. It grows in Korea, Japan, Manchuria, Ussuri River basin, Sakhalin, etc., and is known to be eaten raw or eaten raw with young leaves and fruits or juice, fruit wine, and jam.

The Meurugeun and Mihu, etc. are known as herbal medicines, and may be used by using those collected from nature or by purchasing commercially available ones, but is not limited thereto.

In the present invention, the term "extract" refers to a substance obtained by extracting a certain substance, and specifically, an extract obtained by extracting any one or more oriental medicines selected from the group consisting of Meurugeun and Mihu, etc. of the present invention. , A dilution or concentrate of the extract, a dried product obtained by drying the extract, a prepared or purified product of the extract, or a mixture thereof, and extracts of all formulations that can be formed using the extract itself and the extract.

The method of extracting the herbal medicine is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include a solvent extraction method, an ultrasonic extraction method, a filtration method, a reflux extraction method, and the like, and these may be performed alone or in combination of two or more methods.

In the present invention, the type of the extraction solvent used for the extraction is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, and these may be used alone or in combination of one or more. In the present invention, the extraction solvent may be methanol.

As used herein, the term "fraction" refers to a result obtained by performing fractionation in order to separate a specific component or a specific component group from a mixture including various components.

The fractionation method for obtaining the fraction in the present invention is not particularly limited thereto, as long as it can exhibit a skin wrinkle improvement effect, and may be performed according to a method commonly used in the art. For example, the method includes a solvent fractionation method performed by treating various solvents, an ultrafiltration fractionation method performed by passing an ultrafiltration membrane having a constant molecular weight cut-off value, and various chromatography (size, charge, hydrophobicity or affinity). It may be a chromatographic fractionation method for performing the separation according to the present invention), and combinations thereof.

In the present invention, the type of the fractionation solvent used to obtain the fraction is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the fractionation solvent include polar solvents such as water, distilled water, and alcohol; and non-polar solvents such as hexane, ethyl acetate, chloroform, and dichloromethane. These may be used alone or in combination of two or more. In the case of using an alcohol in the fractionation solvent, an alcohol having 1 to 4 carbon atoms may be used.

In the present invention, the active ingredient may be any one or more extracts selected from the group consisting of Murugeun and Mihudung, and specifically, any one of a Myurugeun extract, a Mihudung extract, or a mixture of a Myurugeun extract and Mihudeung extract. may be, but is not limited thereto.

The cosmetic composition of the present invention may include 0.0001 to 10% by weight of the extract relative to the total cosmetic composition, specifically 0.0005 to 5% by weight.

When the content is less than 0.0001% by weight, it is not preferable because the original desired activity cannot be sufficiently achieved, and when it exceeds 10% by weight, an increase in a distinct effect is not expected as much as an increasing content, which may be uneconomical.

As used herein, the term "skin wrinkle" refers to fine lines caused by skin deterioration, and may be caused by genetic causes, reduction of collagen and elastin present in the skin dermis, external environment, and the like.

As used herein, the term "improvement" refers to suppressing or inhibiting the symptoms of wrinkles generated on the skin using the composition of the present invention, or alleviating the symptoms of skin wrinkles that have already appeared.

In the present invention, the composition may inhibit the activity of elastase (Elastase). The elastase refers to an enzyme having a role of catalyzing the hydrolysis reaction of elastin involved in skin elasticity.

In the present invention, the composition may inhibit the activity of collagenase. The collagenase refers to an enzyme having a role of catalyzing the hydrolysis reaction of collagen present in the skin dermis.

In the present invention, the cosmetic composition comprises a solution, an external ointment, a cream, a foam, a nourishing lotion, a softening lotion, a pack, a softening water, an emulsion, a makeup base, an essence, a soap, a liquid detergent, a bath agent, a sunscreen cream, a sun oil, Suspensions, emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, patches and sprays may be prepared in a formulation selected from the group consisting of, but not limited

The cosmetic composition of the present invention may further include one or more cosmetically acceptable carriers to be formulated in general skin cosmetics, and conventional ingredients include, for example, oil, water, surfactant, humectant, lower alcohol, thickener, A chelating agent, a colorant, a preservative, a fragrance, etc. may be appropriately mixed, but the present invention is not limited thereto.

The cosmetically acceptable carrier included in the cosmetic composition of the present invention varies depending on the formulation of the cosmetic composition.

When the formulation of the present invention is an ointment, paste, cream or gel, as a carrier component, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. may be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc. may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohard propellants such as, but not limited to, locarbon, propane/butane or dimethyl ether. These may be used alone or in combination of two or more.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer, or emulsifier may be used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butylglycol oil and the like can be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic esters, fatty acid esters of polyethylene glycol or sorbitan may be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a suspension, as a carrier component a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters; Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a soap, alkali metal salt of fatty acid, fatty acid hemiester salt, fatty acid protein hydrolyzate, isethionate, lanolin derivative, aliphatic alcohol, vegetable oil, glycerol, sugar, etc. may be used as carrier components. However, it is not limited thereto. These may be used alone or in combination of two or more.

Another aspect of the present invention provides a quasi-drug composition for skin wrinkle improvement comprising as an active ingredient any one or more extracts or fractions thereof selected from the group consisting of Meurugeun and Mihudung.

The meurugeun, mihudung, extract, fraction and skin wrinkle improvement are the same as described above.

The term "quasi-drug" as used in the present invention refers to items with a milder action than pharmaceuticals among items used for the purpose of diagnosing, treating, improving, alleviating, treating or preventing diseases of humans or animals, for example, in the Pharmaceutical Affairs Act. According to the report, quasi-drugs exclude products used for pharmaceutical purposes, and include products used for the treatment or prevention of diseases in humans and animals, and products with minor or no direct action on the human body.

The quasi-drug composition of the present invention may be prepared selected from the group consisting of body cleanser, foam, soap, mask, ointment, cream, lotion, essence and spray, but is not limited thereto.

When the composition according to the present invention is used as a quasi-drug additive, the composition may be added as it is or used together with other quasi-drugs or quasi-drug components, and may be appropriately used according to a conventional method. The mixing amount of the active ingredient may be suitably determined according to the purpose of use.

Another aspect of the present invention provides a method for improving skin wrinkles, comprising administering to an individual a composition comprising any one or more extracts or fractions thereof selected from the group consisting of Meurugeun and Mihudung as an active ingredient.

The meurugeun, mihudung, extract, fraction and skin wrinkle improvement are the same as described above.

As used herein, the term "administration" means introducing the composition of the present invention to a patient by any suitable method, and the administration route of the composition may be administered through any general route as long as it can reach the target tissue. have. For example, intraperitoneal administration, intravenous administration, intramuscular administration, subcutaneous administration, intradermal administration, oral administration, topical administration, may be administered intranasally, but is not limited thereto. Since the composition of the present invention is effective in improving skin wrinkles, the route of administration of the composition may be administered by applying to the skin.

The composition of the present invention may be administered daily or intermittently, and the number of administrations per day may be administered once or divided into two to three times. In addition, the composition of the present invention may be used alone or in combination with other drug treatments for antioxidation, whitening, skin aging or skin wrinkle improvement. Taking all of the above factors into consideration, it is important to administer an amount that can obtain the maximum effect with a minimum amount without side effects, and can be easily determined by those skilled in the art.

As used herein, the term "individual" refers to all animals, such as rats, mice, and livestock, including humans, which have or can develop skin wrinkles or skin aging. Specifically, it may be a mammal including a human.

Any one or more extracts or fractions thereof selected from the group consisting of Vitis amurensis Ruprecht and Actinidia arguta according to the present invention have excellent skin wrinkle improvement effects, and can be applied as a cosmetic composition for skin wrinkle improvement. have.

1 is a diagram showing the elastase inhibitory activity of Myurugeun extract (* p<0.005).
Figure 2 is a diagram showing the elastase inhibitory activity of the extract of Mihudeung (* p<0.005).
3 is a diagram showing the elastase inhibitory activity of olenic acid (* p<0.005).
Figure 4 is a diagram showing the collagenase inhibitory activity of EGCG, Mihudeung extract, and Meurugeun extract (* p<0.05, ** p<0.005).

Hereinafter, the present invention will be described in more detail by way of Examples. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited thereto.

Example 1. Preparation of herbal extracts

Vitis amurensis Ruprecht, Actinidia arguta were purchased from Korea Herbal Medicine (Korea). After weighing 10 g of dry weight of each herbal medicine and pulverizing, 5 times the amount of methanol solvent was added to the sample, and extraction was repeated three times at 65°C. Each extracted sample was freeze-dried (FDU-2100, EYELA, Japan), and the yield was calculated by the gravimetric method.

Example 2. Elastase (Elastase) inhibitory activity measurement

In order to confirm the elastase activity inhibitory effect of each herbal extract prepared in Example 1, an elastase inhibition assay was performed. Using STANA (N-Succinyl-tri(Ala)-p-nitroanilide, Sigma, USA) as a substrate, the amount of p-nitroanilide produced from the substrate at 37°C for 30 minutes was measured at 410 nm. . For elastase, porcine pancreatic elastase (Sigma-Aldrich, St. Louis, MO. USA) was used. Specifically, in 50 mM Tris-HCl buffer (pH 8.6), each concentration (12.5, 25, 50, 100, 200, 250, 500, 1,000 μg/ml) 50 μL of each herbal extract, 25 μL of pig pancreatic elastase ( 2,500 U/mL) and 25 μL of 8.8 mM STANA, a substrate, were mixed and reacted at 37° C. for 30 minutes, and then absorbance was measured at 410 nm. At this time, the negative control group was used with Tris-HCl buffer instead of the sample, and the elastase activity inhibitory effect was calculated by subtracting the absorbance of the blank test without the enzyme. In this case, the standard material (positive control) was used as oleanolic acid (Oleanolic acid, Sigma, USA). The elastase inhibition rate was calculated as follows.

Elastase inhibition rate (%) = 1 (OD of sample) / OD of negative control × 100

As a result, it was confirmed that the elastase inhibitory ability increased as the concentration of each herbal extract was increased as shown in FIGS. 1 to 2 .

In addition, as shown in Table 1 below, the concentration (IC ₅₀ ) of the extract when the inhibitory ability is 50% was calculated, and as a result, it was found to be 446.6 μg/mL for meurugeus root and 177.8 μg/mL for Mihu, respectively. It was confirmed that the material was in stock. In the case of mulberry root, it was _{slightly higher than the IC 50} (134.6 μg/ml) of olenic acid used as a positive control, but it was confirmed that it exhibited strong elastase inhibitory ability considering that it was an herbal extract.

extract IC ₅₀ (μg/ml) V. amurensis Ruprecht 446.6 A. arguta (tail lamp) 177.8 olenic acid 134.6

Example 3. Collagenase (Collagenase) inhibitory activity measurement

In order to confirm the collagenase activity inhibitory effect of each herbal extract prepared in Example 1, a collagenase inhibition assay was performed according to the method of Wunsch E and Heindrich HG. _{Specifically, by adding 4 mM CaCl 2} to 0.1 M Tris-HCl buffer (pH 7.5), 4-phenylazobenzyloxycarbonyl-proline-leucine-glycine-proline-arginine (4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly- Pro-Arg) 0.3 mg/mL was added to a mixture of 0.25 mL of substrate solution and 0.1 mL of each herbal extract by concentration, and 0.15 mL of 0.2 mg/mL collagenase was added, followed by reaction at room temperature for 20 minutes. To stop the reaction, 0.5 mL of 6% citric acid was added, 1.5 mL of ethyl acetate was added, and then centrifuged at 3,000 rpm for 10 minutes to collect only the supernatant and measure the absorbance at 320 nm. At this time, the negative control group used Tris-HCl buffer instead of the sample, and EGCG (Epigallocatechin gallate) was used as the standard (positive control group), and the collagenase inhibitory activity was expressed as the absorbance reduction rate of the extract-treated group and the negative control group. It was. The measured value was expressed as an average value of three repeated experiments.

Collagenase inhibitory activity (%) = (1-A/B) × 100

A: absorbance of the sample at 320 nm

B: Absorbance of negative control at 320 nm

As a result, as shown in FIG. 4 , it was confirmed that the collagenase activity was inhibited as the concentration of each extract increased.

_{In addition, as shown in Table 2 below, as a result of calculating the concentration (IC 50} ) of the extract when the inhibitory ability is 50%, it was found to be 44.4 µg/mL for tail light and 26.7 µg/mL for Meurus root, IC _{50 of} EGCG used as a positive control ( 45.4 μg/ml) was confirmed.

From these results, it was confirmed that the collagenase inhibitory activity of each herbal extract was very excellent, and it was confirmed that it was a material having the effect of improving skin wrinkles.

extract IC ₅₀ (μg/ml) A. arguta (tail lamp) 44.4 V. amurensis Ruprecht 26.7 EGCG 45.4

From the above description, those skilled in the art to which the present invention pertains will understand that the present invention may be embodied in other specific forms without changing the technical spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention, rather than the above detailed description, all changes or modifications derived from the meaning and scope of the claims described below and their equivalents.

Claims (7)

A cosmetic composition for improving skin wrinkles comprising any one or more extracts or fractions thereof selected from the group consisting of Vitis amurensis Ruprecht and Actinidia arguta as an active ingredient.
The cosmetic composition according to claim 1, wherein the extract is prepared by extraction with a solvent selected from the group consisting of water, alcohols having 1 to 4 carbon atoms, and mixed solvents thereof.
The cosmetic composition of claim 1, wherein the extract comprises 0.0001 to 10% by weight of the total cosmetic composition.
The cosmetic composition according to claim 1, wherein the composition inhibits the activity of elastase.
The cosmetic composition according to claim 1, wherein the composition inhibits the activity of collagenase.
A quasi-drug composition for skin wrinkle improvement comprising as an active ingredient any one or more extracts or fractions thereof selected from the group consisting of Meorugun and Mihu, etc.
A method for improving skin wrinkles, comprising administering a composition comprising as an active ingredient any one or more extracts or fractions thereof selected from the group consisting of Meorugun and Mihudung, to individuals other than humans.

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