KR20210073341A - Manufacturing method of herbal medicine mixed beverage with improved liver function and palatability and beverage produced by the same method - Google Patents

Abstract

The present invention relates to a method of preparing a medicinal herb mixed beverage having a liver function improving effect and improved palatability, and a beverage prepared by the same. The medicinal herb mixed beverage is prepared by the method including the steps of: washing and mixing medicinal herbs such as Curcuma longa, jujube, Pueraria montana var. lobata, Rehmannia glutinosa, Angelica gigas, Astragalus, Cnidium officinale, Lycium chinense, dry ginger, Wolfiporia extensa, Artemisia campestris, and Hovenia dulcis fruits; and extracting a medicinal herb mixed extract from the mixture of the medicinal herbs by using hot water. The medicinal herb mixed beverage is advantageous of: restoring a liver function damaged by alcohol intake; reducing serum and lipid in the liver tissue, increased by alcohol intake; and being prepared without a bad odor and a bad taste, thereby meeting consumer preferences. Moreover, when extracting a mixture, salted groundwater is separately mixed in addition to the use of general purified water so that the beverage may contain highly concentrated natural dissolved minerals and water-soluble calcium as well as functional materials of the salted groundwater. Therefore, the functional materials can be easily consumed.

Description

There is efficacy in improving liver function, and a method for manufacturing a herbal medicine mixed beverage with improved palatability, and a beverage prepared by the manufacturing method {Manufacturing method of herbal medicine mixed beverage with improved liver function and palatability and beverage produced by the same method}

The present invention relates to a method for preparing a herbal medicine mixed beverage having improved liver function and improved palatability, and to a beverage prepared by the method.

As one of the main organs of animals, the liver performs various roles such as detoxification, protein synthesis, storage of nutrients such as glycogen, metabolism of fat, production of bile and urea, and breakdown of red blood cells. It has basic control functions for maintaining health.

The liver decomposes, metabolizes and metabolizes various drugs, alcohol, and other toxic substances that can be recognized as toxins in our body and excretes them through urine or bile. In patients with liver cirrhosis, this detoxification effect is lowered, so the risk of drugs, alcohol, and toxic substances may increase. In addition, the liver is an important immune organ and at the same time is responsible for sterilizing the body. Bacteria that are absorbed into the blood through the large intestine mucosa and enter the body pass through the liver and die by the phagocytic cells called Kupffer cells, so only about 1% of the bacteria pass through the liver safely. can go out However, in patients with liver cirrhosis, this function is reduced and it is easy to be infected with various bacteria. A representative example is Vibrio sepsis caused by eating uncooked fish and shellfish in summer, which occurs intensively in patients with liver cirrhosis.

Therefore, it is necessary to make an effort to protect the liver through a lifestyle that does not put strain on the liver, a correct diet, and appropriate exercise. However, modern people have difficulties in maintaining the balance for such health management due to their busy daily life, so it is necessary to consume foods that help maintain the health of the liver.

On the other hand, Korean Patent No. 1930026 discloses a method for producing a black garlic drink that is effective in improving liver function, and Korean Patent No. 1817282 discloses a method for preparing a mixed drink with improved liver function and antioxidant activity. Although disclosed, there is no disclosure with respect to a method for manufacturing a mixed beverage with herbal medicines having an effect on improving liver function and improving palatability of the present invention and a beverage prepared by the manufacturing method thereof.

The present invention has been derived from the above requirements, and herbal medicine mixture obtained by mixing turmeric, jujube, arrowroot, Sukjihwang, angelica, hwanggi, cheongung, gugija, dry ginger, upper extremity, bokryeong, wormwood and earth worm followed by hot water extraction. By providing a method for producing an extract, and confirming that a beverage containing a herbal medicine mixed extract prepared by the above method is effective in improving liver function and improving palatability, the present invention has been completed.

In order to solve the above problems, the present invention

1) after washing turmeric, jujube, arrowroot, sukjihwang, angelica, hwanggi, cheongung, goji berry, dried ginger, upper extremity, bokryeong, injin mugwort and earth worm, mixing the washed herbal material with water; and

2) hot water extraction of the herbal medicine mixture of step 1) is effective in improving liver function, including; and provides a method for preparing a herbal medicine mixture beverage with improved palatability.

In addition, the present invention is effective in improving liver function prepared by the above manufacturing method, and provides a herbal medicine mixed beverage with improved palatability.

The present invention relates to a method for manufacturing a herbal medicine blend beverage having improved liver function and improved palatability and a beverage prepared by the method, and relates to turmeric, jujube, arrowroot, Sukjihwang, Angelica, Hwanggi, cheongung, gugija, and dry ginger. After washing the upper extremities, bokryeong, wormwood, and earth worm, the herbal medicines are mixed and then hot water extraction to obtain a herbal medicine mixture extract; herbal medicine mixture beverages prepared including alcohol treatment promotes reduced liver cell regeneration rate In animal experiments, it reduces the blood alcohol content and acetaldehyde content increased by alcohol ingestion, and reduces the activity of aspartate transaminase (AST) and alanine transaminase (ALT) in the blood increased by alcohol intake. It was confirmed that increased the activity of ADH (alcohol dehydrogenase) and ALDH (aldehyde dehydrogenase) in liver tissue decreased by alcohol intake, and decreased serum and liver tissue lipids increased by alcohol intake. In addition, the beverage of the present invention, which has confirmed the liver function improvement effect, has the advantage that it does not have off-flavors and images, and conforms to the preferences of consumers.

Moreover, when extracting the mixture, in addition to using general purified water, highly concentrated natural dissolved minerals and water-soluble calcium can be included in the beverage by additionally mixing salted groundwater, and the functional substance of salted sewage can be easily consumed by including the functional material in the beverage. There is this.

1 is a result of confirming the cell regeneration ability for alcohol damage of the herbal medicine mixed beverage of the present invention. CON is a negative control and silymarin is a positive control. Experimental Example 1 is a beverage intake group containing a herbal medicine mixed extract extracted using general purified water, and Experimental Example 2 is a beverage intake group containing a herbal medicine mixture extract extracted using purified water containing saline sewage.
Figure 2 is the result of confirming the change in blood alcohol concentration (A) and acetaldehyde concentration change (B) by the herbal medicine mixed drink of the present invention in an animal model ingesting alcohol. NC is a negative control, EtOH CON is an alcohol-only control, and silymarin is a positive control. Experimental Example 1 is a beverage intake group containing a herbal medicine mixed extract extracted using general purified water, and Experimental Example 2 is a beverage intake group containing a herbal medicine mixture extract extracted using purified water containing saline sewage.
3 is a result of measuring changes in blood AST activity (A) and ALT activity change (B) by the herbal medicine mixed drink of the present invention in an animal model ingesting alcohol. NC is a negative control, EtOH CON is an alcohol-only control, and silymarin is a positive control. Experimental Example 1 is a beverage intake group containing a herbal medicine mixed extract extracted using general purified water, and Experimental Example 2 is a beverage intake group containing a herbal medicine mixture extract extracted using purified water containing saline sewage.
4 is a result of measuring the change in blood ADH activity (A) and ALDH activity change (B) by the herbal medicine mixed drink of the present invention in an animal model ingesting alcohol. NC is a negative control, EtOH CON is an alcohol-only control, and silymarin is a positive control. Experimental Example 1 is a beverage intake group including a herbal medicine mixture extract extracted using normal purified water, and Experimental Example 2 is a beverage intake group containing a herbal medicine mixture extract extracted using purified water containing saline sewage.

the present invention

1) after washing turmeric, jujube, arrowroot, sukjihwang, angelica, hwanggi, cheongung, goji berry, dried ginger, upper extremity, bokryeong, injin mugwort and earth worm, mixing the washed herbal material with water; and

2) hot water extraction of the herbal medicine mixture of step 1) is effective in improving liver function, including; and relates to a method for producing a herbal medicine mixture beverage with improved palatability.

The mixing of step 1) is 0.5~1.5kg of turmeric, 0.25~0.75kg of jujube, 0.25~0.75kg of arrowroot, 0.05~0.15kg of shuangihuang, 0.05~0.15kg of angelica, 0.05~0.15kg of Astragalus, 0.05~0.15kg of cheongung per 100L of water. 0.15kg, 0.1~0.2kg Goji berry, 0.1~0.3kg dry ginger, 0.5~1.5kg upper extremity, 0.05~0.15kg bokryeong, 0.02~0.07kg Injin mugwort, and 0.25~0.75kg of earth berry, preferably 100L of water About turmeric 0.8~1.2kg, jujube 0.4~0.6kg, arrowroot 0.4~0.6kg, sukhumang 0.08~0.12kg, angelica 0.08~0.12kg, astragalus 0.08~0.12kg, cheongung 0.08~0.12kg, wolfberry 0.13~0.17kg, 0.15 to 0.25 kg of dried ginger, 0.8 to 1.2 kg of upper limb, 0.08 to 0.12 kg of bokryeong, 0.04 to 0.06 kg of wormwood, and 0.4 to 0.6 kg of earthworm, more preferably 1 kg of turmeric, 0.5 kg of jujube per 100 L of water , arrowroot 0.5kg, Sukhumi Hwang 0.1kg, Angelica 0.1kg, Astragalus 0.1kg, Chunkyung 0.1kg, Goji berry 0.15kg, dry ginger 0.2kg, upper extremity 1kg, Bokryeong 0.1kg, Injin mugwort 0.05kg, and Earth berry 0.5kg. The present invention is not limited thereto.

The hot water extraction in step 2) may be extraction at 80-120° C. for 3-5 hours, preferably at 90-110° C. for 3-5 hours, and more preferably at 100° C. for 4 hours. During extraction, but is not limited thereto.

In one embodiment of the present invention, the water in step 1) may be pure water or water mixed with saltwater, preferably purified water or purified water containing 5-15% (v/v) saltwater. It can be used, and more preferably, purified water containing 10% (v/v) salted sewage is used, but is not limited thereto.

The term 'saltwater' as used in the present invention is produced by combining fresh water permeated from the land and seawater flowing from the sea, and has a total dissolved solids content such as salt dissolved in the water of 2,000 mg/ It is groundwater in rock aquifers above L.

Saltwater has a high mineral content, and the water temperature is 16~18℃ and the pH is 7.3~7.5, so it has low-temperature stability and physical advantages with little seasonal variation and characteristic change. It is not exposed to the external environment, so it is free from harmful components such as general bacteria and heavy metals. There is no clean water resource.

The salt sewage of the present invention may be anywhere as long as it can be obtained. Preferably, salt sewage obtained from Uljin-gun, Gyeongsangbuk-do was used.

The herbal medicine mixed drink is after step 2), 0.1 to 0.5 parts by weight of taurine, 0.05 to 0.15 parts by weight of guarana extract powder, and 1.5 to 2.5 parts by weight of specification honey based on 100 parts by weight of the herbal medicine mixed hot water extract. Preferably, 0.2 to 0.4 parts by weight of taurine, 0.08 to 0.12 parts by weight of guarana extract powder, and 1.9 to 2.3 parts by weight of specification honey are further mixed with respect to 100 parts by weight of herbal medicine mixed hot water extract, more preferably herbal medicine mixture With respect to 100 parts by weight of the hot water extract, 0.33 parts by weight of taurine, 0.11 parts by weight of guarana extract powder, and 2.18 parts by weight of specification honey are further mixed, but is not limited thereto.

In one embodiment of the present invention, the herbal medicine mixed beverage having an effect on improving liver function and improving palatability is preferably

1) After washing turmeric, jujube, arrowroot, Sukjihwang, angelica, hwanggi, cheongung, gugija, dried ginger, upper extremity, bokryeong, injin mugwort and earth worm, 0.5~1.5kg of washed turmeric, 0.25~0.75 jujube per 100L of water kg, arrowroot 0.25~0.75kg, red sagebrush 0.05~0.15kg, angelfish 0.05~0.15kg, astragalus 0.05~0.15kg, cheongung 0.05~0.15kg, wolfberry 0.1~0.2kg, dry ginger 0.1~0.3kg, upper extremity 0.5~1.5kg , mixing bokryeong 0.05-0.15kg, injin mugwort 0.02-0.07kg and gujaja 0.25-0.75kg; and

2) extracting the herbal medicine mixture of step 1) with hot water at 80 to 120° C. for 3 to 5 hours; can be prepared including,

more preferably

1) After washing turmeric, jujube, arrowroot, sukjihwang, angelica, hwanggi, cheongung, gugija, dried ginger, upper extremity, bokryeong, injin mugwort and earth worm, 0.8~1.2kg of washed turmeric, 0.4~0.6 of jujube per 100L of water kg, kudzu 0.4~0.6kg, red sagebrush 0.08~0.12kg, angelica 0.08~0.12kg, astragalus 0.08~0.12kg, cheongung 0.08~0.12kg, wolfberry 0.13~0.17kg, dry ginger 0.15~0.25kg, upper extremity 0.8~1.2kg , mixing bokryeong 0.08-0.12kg, injin mugwort 0.04~0.06kg and guinea pig 0.4-0.6kg; and

2) extracting the herbal medicine mixture of step 1) with hot water at 90 to 110° C. for 3 to 5 hours; can be prepared including,

even more preferably

1) After washing turmeric, jujube, kudzu, sukjihwang, angelica, hwanggi, cheongung, goji berry, dried ginger, upper extremity, bokryeong, injin mugwort and earth worm, 1 kg of washed turmeric, 0.5 kg of jujube, 0.5 kg of arrowroot per 100L of water , Sukjihwang 0.1kg, Angelica 0.1kg, Astragalus 0.1kg, Chrysanthemum 0.1kg, Goji berry 0.15kg, dry ginger 0.2kg, upper extremity 1kg, Bokryeong 0.1kg, Injin mugwort 0.05kg, and Astragalus 0.5kg; and

2) hot water extraction of the herbal medicine mixture of step 1) at 100° C. for 4 hours; but is not limited thereto.

In addition, the present invention relates to a herbal medicine mixed beverage having an effect on improving liver function and having improved palatability prepared by the above manufacturing method.

The beverage has an effect of improving liver function, and there is no off-flavor and no image, so that consumer preference is improved.

Hereinafter, the present invention will be described in more detail using Preparation Examples and Examples. These preparations and examples are only for illustrating the present invention in more detail, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited thereto.

Preparation Example 1. Preparation of herbal medicine mixture extract according to compounding materials

For the preparation of herbal medicine mixed drinks, herbal medicines were mixed as shown in Table 1 below, followed by hot water extraction at 100° C. for 4 hours.

material (unit) Experimental Example 1 Comparative Example 1 Comparative Example 2 Purified water (L) 100 100 100 Turmeric (kg) 1.00 0.6 - Jujube (kg) 0.50 - 0.60 kudzu(grilled root)(kg) 0.50 0.6 1.00 Sukji Hwang (Dried Hwang) (kg) 0.10 - - Angelica (kg) 0.10 0.5 - Astragalus (kg) 0.10 0.5 - celestial palace (kg) 0.10 - 0.50 Goji (kg) 0.15 - 1.00 Dried ginger (kg) 0.20 0.6 - Upper extremity (kg) 1.00 0.6 - Bokryeong (kg) 0.10 - 0.50 Injin Wormwood (kg) 0.05 1.00 0.80 Earth ruler (kg) 0.50 - -

Preparation Example 2. Preparation of herbal medicine extract according to the mixing ratio

To prepare a herbal medicine mixed beverage according to the mixing ratio, herbal medicines were mixed according to the mixing ratio as shown in Table 2 below, followed by hot water extraction at 100° C. for 4 hours.

material (unit) Experimental Example 1 Comparative Example 3 Comparative Example 4 Purified water (L) 100 100 100 Turmeric (kg) 1.00 0.50 1.50 Jujube (kg) 0.50 0.80 0.10 kudzu(grilled root)(kg) 0.50 0.20 0.40 Sukji Hwang (Dried Hwang) (kg) 0.10 0.15 0.05 Angelica (kg) 0.10 0.05 0.25 Astragalus (kg) 0.10 0.10 0.08 celestial palace (kg) 0.10 0.20 0.10 Goji (kg) 0.15 0.50 0.30 Dried ginger (kg) 0.20 0.30 0.10 Upper extremity (kg) 1.00 1.00 0.50 Bokryeong (kg) 0.10 0.10 0.10 Injin Wormwood (kg) 0.05 0.10 0.02 Earth ruler (kg) 0.50 0.40 0.90

Preparation Example 3. Preparation of herbal medicine extract using purified water to which salted groundwater was added

An herbal medicine extract was prepared in the same formulation as in Experimental Example 1, but hot water extraction was performed using purified water containing 10% (v/v) salted groundwater (Experimental Example 2).

The salted sewage used in the present invention contains 1168.7 mg of calcium ions, 1001 mg of magnesium ions, 103.4 mg of potassium ions, and 8757.7 mg of sodium ions per 1 L of salted sewage.

After preparing an herbal medicine extract using purified water with added saltwater, as a result of requesting component analysis, sodium and calcium without addition were measured at 56.4mg/100ml and 19.6mg/100ml, respectively, which means that water-soluble sodium and calcium are It is presumed to originate from the bedrock water.

Preparation Example 4. Preparation of herbal medicine mixed beverage in which taurine, guarana extract powder and specification honey are mixed

0.33 parts by weight of taurine, 0.11 parts by weight of guarana extract powder, and 2.18 parts by weight of specification honey were further mixed with respect to 100 parts by weight of herbal medicine mixed hot water extract prepared in the same formulation as in Experimental Example 1 to prepare a herbal medicine mixed drink (Experimental Example 3).

The powder of the guarana extract is obtained by pulverizing the guarana hot water extract and mixing it with dextrin.

Example 1. Liver function improvement effect of herbal medicine mixed beverage

The liver function improvement effect of the herbal medicine mixture extract of Experimental Example 1 and Experimental Example 2 was confirmed.

1) Measurement of regenerative activity of liver cells damaged by alcohol

The normal liver cells (Chang cells) used in the experiment were purchased from the Korea Cell Line Bank (KCLB) and cultured in a DMEM medium supplemented with 10% (v/v) FBS at 37° C., 5% CO ₂ incubator. did.

Cell regeneration activity was confirmed by measuring the cell viability, and the cell viability was measured using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) analysis method.

^{Specifically, the cells were equally distributed at 1×10 5} cells/well in a 96-well plate, and after culturing for 24 hours, each sample was added to the medium to a concentration of 100 μg/mL, and then at 37° C., 5% CO ₂ Cultivation was carried out in an incubator for 24 hours. All groups except for the negative control group (CON) were treated with 5% (v/v) ethanol, and after 24 hours, the supernatant was removed, MTT solution was added, and further incubated for 3 hours. Thereafter, the supernatant was removed, 200 μl of dimethyl sulfoxide (DMSO) was added, and then insoluble formazan crystal was dissolved, and the absorbance of the lysate was measured at 570 nm to calculate cell viability. Measurement results were expressed as relative cell viability with respect to the negative control group.

As a result, as shown in Figure 1, Experimental Example 1 and Experimental Example 2 of the present invention increase the cell viability decreased by the addition of ethanol, and thus it was confirmed that there is a liver cell regeneration activity.

2) Confirmation of liver function improvement effect in animal model consuming alcohol

- Experimental animals and classification

Five-week-old (130-150 g) SD (Sprague-Dawley) male rats were distributed from Orient Bio (Gyeonggi-do, Korea) and acclimatized for one week. Then, at the age of 6 weeks, the groups were separated by a randomized block design.

The temperature of the breeding room was 22℃ and the humidity was 55%, and the breeding was carried out in an environment that maintains daylight control from 6:00 to 18:00. During the adaptation period, the animal's peculiarities were observed and recorded, and food and water were freely ingested.

- How to treat the experimental group

The experimental group was a negative control group (NC), an alcohol administration group (EtOH CON), a positive control group administered with ethanol and silymarin 2.5 or 5 mg/kg BW, Experimental Example 1 of the present invention (a beverage containing a herbal medicine mixed extract extracted using general purified water) ) and Experimental Example 2 (a beverage containing a herbal medicine mixed extract extracted using purified water containing salted groundwater) was classified into a group administered with 250 or 500 mg/kg BW.

Alcohol and test substance were orally administered at a dose of 2 mL per kg body weight of white rats. In addition, in order to exclude a phenomenon that interferes with the absorption of alcohol through the gastrointestinal tract, which may occur due to feed intake, after fasting for 18 hours before oral administration of alcohol, after treatment with 20% (v/v) EtOH for 1 week After 30 minutes, the test substance was fed, and on the second week after 40% (v/v) EtOH was treated, the test substance was fed 30 minutes later.

To measure blood ethanol concentration, acetaldehyde concentration and enzyme activity, after administration on the 14th day, fasting for 18 hours, alcohol and each sample were orally administered on the 15th day. was collected. The collected blood was centrifuged at 4° C., 3,000 rpm for 15 minutes to separate serum, alcohol and acetaldehyde concentrations, AST, AST, ADH and ALDH enzyme activities were measured from the separated serum, and lipid content was analyzed.

The liver tissue of the extracted experimental animal was washed several times in PBS (phosphate buffered saline) solution to remove foreign substances on the surface, then weighed, quenched in liquid nitrogen and stored at -70°C until sample analysis. Lipid content of liver tissue used for analysis.

- result

① Measurement of blood alcohol and acetaldehyde

Blood alcohol concentration was analyzed using a kit for ethanol measurement (R-Biopharm, Germany) prepared using the method of Bucher (1951). After dissolving 1 tablet of NAD (0.8 mg) in 3 ml of potassium diphosphate buffer (pH 9.0), 0.1 mL of serum was added and reacted for 3 minutes. After that, the absorbance value measured at 340 nm was referred to as A1, and 0.05 mL of ADH was added and reacted for 5 minutes. Then, the absorbance value measured at 340 nm was referred to as A2, and the alcohol concentration was calculated as in Equation 1 below.

[Equation 1]

Alcohol (mmol/L)=[(V×MW)/(ε×d×v×2×1000)]×ΔA

V: final volume

v: sample volume

MW: molecular weight of the substrate used in the analysis

d: light path

ε: Extinction coefficient of NADH at 340 nm = 6.3

ΔA: (A2-A1) sample-(A2-A1) blank

Serum acetaldehyde concentration was analyzed using the acetaldehyde measurement kit (R-Biopharm, Germany) prepared using the method of Lundquist (1974). After dissolving 1 tablet of NAD (0.8 mg) in 3 ml of potassium diphosphate buffer (pH 9.0), 0.2 mL of serum was added and reacted for 3 minutes. After that, the absorbance value measured at 340 nm was called A1, and 0.05 mL of ALDH was added and reacted for 5 minutes. The absorbance value measured at 340 nm was called A2, and the acetaldehyde concentration was calculated in the same manner as in the alcohol concentration calculation formula.

As a result, as shown in FIG. 2 , it was confirmed that the alcohol concentration and acetaldehyde concentration increased by the alcohol administration were decreased by the treatment of Experimental Examples 1 and 2 of the present invention.

② Measurement of AST and ALT enzyme activity in blood

Blood AST was measured using the AST-ALT measurement kit (AM 101, Asan, Korea) prepared using the method of Reitman-Frankel (1957). 1 mL of substrate solution (α-ketoglutamic acid, L-aspartic acid) was pre-reacted at 37°C for 5 minutes, 0.2 mL of serum was added, and reacted at 37°C for 1 hour, followed by 1 mL of coloring reagent (2,4-dinitro phenylhydrazine) was added and left at room temperature for 20 minutes, the reaction was stopped by adding 10 mL of 0.4N NaOH, and then the absorbance was measured at 505 nm with distilled water as a control. The AST activity value was calculated based on the calibration curve of the standard solution.

Blood ALT was measured using the AST-ALT measurement kit (AM 101, Asan, Korea) prepared using the method of Reitman-Frankel (1957). 1 mL of substrate solution (α-ketoglutamic acid, L-aspartic acid) was pre-reacted at 37°C for 5 minutes, 0.2 mL of serum was added, and reacted at 37°C for 1 hour, followed by 1 mL of coloring reagent (2,4-dinitro phenylhydrazine) was added and left at room temperature for 20 minutes, the reaction was stopped by adding 10 mL of 0.4N NaOH, and then the absorbance was measured at 505 nm with distilled water as a control. ALT activity was calculated based on the calibration curve of the standard solution.

As a result, as shown in FIG. 3 , it was confirmed that the AST and ALT activities increased by alcohol administration were decreased by the treatment of Experimental Examples 1 and 2 of the present invention.

③ Measurement of ADH and ALDH enzyme activity in blood

ADH activity in liver tissue was measured by Bergmeyer's method (1974). To 0.2M NaOH/glycine buffer (pH 9.6), 0.67mM NAD solution and 0.05mL of an enzyme solution were added, followed by pre-reaction at 37°C for 5 minutes. Thereafter, 100 mM ethanol was added and mixed, and the absorbance was immediately measured at 340 nm. After reacting the solution at 37° C. for 5 minutes, the amount of NADH production was measured by absorbance at 340 nm, and the activity was calculated using a standard calibration curve. The enzyme activity was expressed in nmol as the amount of NADH produced by 1 mg of protein per minute.

ALDH activity was measured using the Koivula and Koivusalo method (1975). 13.3mM NAD solution, 16.7mM pyrazole solution and 0.05mL of enzyme solution were added to 0.1M tetrasodium pyrophosphate decahydrate buffer, and the reaction was carried out at 37°C for 5 minutes. Absorbance was measured at 340 nm immediately after addition of 60 mM propionaldehyde solution. Then, the solution was reacted again at 37° C. for 5 minutes to measure the amount of NADH produced by absorbance at 340 nm after completion of the reaction, and the activity was calculated using a standard calibration curve. The enzyme activity was expressed in nmol as the amount of NADH produced by 1 mg of protein per minute.

As a result, as shown in FIG. 4 , it was confirmed that the ADH and ALDH activities, which were decreased by alcohol administration, were increased by the treatment of Experimental Examples 1 and 2 of the present invention.

④ Measurement of lipid content in blood and liver tissue

Total cholesterol in blood and liver tissue was measured using a reagent (AM 202-K, Asan Pharmaceutical) that applied the enzyme method of Allain et al. (1974). Since serum cholesterol exists in two forms, CE (cholesterol ester) and free cholesterol, in order to quantify both, ester-type cholesterol in serum is converted to cholesterol esterase, and fatty acid and free cholesterol are converted to cholesterol oxidase. (cholesterol oxidase) to Δ4-cholestenon, a mixture of peroxidase and substrate H ₂ O ₂ , phenol, and 4-amino-antipyrine to develop a red color. After measuring the absorbance at 500 nm, the standard curve for cholesterol was compared with .

Liver tissue cholesterol was used by modifying the method of Folch et al. (Folch et al., 1957). After taking 1 g of liver tissue, it was homogenized with a 6 mL chloroform: methanol (2:1, v/v) solution and 2 mL distilled water, and the homogenate was centrifuged at 4 ° C, 1,000 rpm for 10 minutes to obtain a lower layer (chloroform layer). After acquisition, it was used for analysis. After taking 500 μl of the lower layer, it was dried naturally for 24 hours, and 50 μl tryptone X100: chloroform (1:1, v/v) solution was added and mixed. Then, after dilution with 450 μl chloroform, the total amount was 500 μl, and 10 μl of this solution was dried for 12 hours and used for measurement.

Quantification of neutral lipids in serum and liver tissue lipid extracts was measured with kit reagent (AM 157S-K, Asan Pharmaceutical) for quantification by enzymatic method. The liver tissue lipid extract was used in the same manner as the total cholesterol quantification, and 10 µl of the lower supernatant was dried naturally for 12 hours and then dissolved in 50 µl methanol and quantified with a kit.

Quantification of HDL-C (High density lipoprotein cholesterol) in serum and liver tissue was measured with a kit reagent (AM 203, Asan Pharmaceutical) for quantification by enzymatic method. The liver tissue lipid extract was extracted and used in the same method as the quantification of total cholesterol, and was quantified by comparison with a standard cholesterol solution.

Quantification of LDL-C (Low Density Lipoprotein Cholesterol) in serum and liver tissue is calculated by the Friedewald formula when the triglyceride content is 400 mg/dL or more, so it was calculated by Equation 2 below.

[Equation 2]

LDL-C=TC-[HDL-C+(TG/5)

Arteriosclerosis index (Athrogenic index, AI) was calculated by Equation 3 below.

[Equation 3]

AI=[(TC-HDL-C)]/HDL-C

As a result, as shown in Table 3 below, blood TG, TC, LDL-C and arteriosclerosis index, which were increased by alcohol administration, were decreased by the treatment of Experimental Examples 1 and 2 of the present invention, and decreased by alcohol administration It was confirmed that the HDL-C was increased by the treatment of Experimental Example 1 and Experimental Example 2 of the present invention.

In addition, as shown in Table 4 below, TG and TC of liver tissue, which were increased by alcohol administration, were decreased by the treatment of Experimental Examples 1 and 2 of the present invention.

Parameter Serum TG
(mg/dL) TC
(mg/dL) HDL-C
(mg/dL) LDL-C
(mg/dL) arteriosclerosis index NC 35.17±1.63 65.74±1.16 35.13±2.27 22.88±1.87 0.87±0.03 EtOH CON 51.32±3.28 80.48±2.28 25.22±2.06 39.15±3.40 2.19±0.08 positive control
(Silymarin) 2.5mg/kg 45.79±1.69 73.23±2.55 29.36±3.84 33.23±1.53 1.49±0.10 5mg/kg 37.30±4.83 65.43±3.98 36.91±3.56 24.57±2.90 0.77±0.06 Experimental Example 1 250mg/kg 45.28±3.77 74.90±4.26 29.03±2.89 31.79±2.99 1.58±0.13 500mg/kg 43.11±2.61 69.98±1.32 32.89±3.13 27.89±3.02 1.13±0.20 Experimental Example 2 250mg/kg 45.32±2.03 75.18±2.01 28.99±2.11 31.29±1.66 1.59±0.09 500mg/kg 42.57±4.28 70.12±3.00 32.11±3.45 28.33±1.83 1.18±0.11

Parameter Liver TG
(mg/g) TC
(mg/g) NC 14.32±0.22 2.81±0.11 EtOH CON 18.01±1.76 4.02±0.12 positive control
(Silymarin) 2.5mg/kg 16.46±1.02 2.69±0.23 5mg/kg 15.63±0.89 2.40±0.31 Experimental Example 1 250mg/kg 16.59±1.23 3.06±0.25 500mg/kg 15.87±0.34 2.98±1.02 Experimental Example 2 250mg/kg 16.23±0.44 2.99±0.08 500mg/kg 15.88±1.06 2.83±0.23

Example 2. Sensory test of herbal medicine mixed beverage

The sensory test of the herbal medicine mixed beverage of the present invention was conducted on a total of 30 subjects in their 30s and 50s, 15 females and 15 males. The sensory test for taste, aroma, color, and overall preference was conducted using a 9-point scale method, and a higher score means a more positive evaluation.

1) Beverage containing herbal medicine mixed extracts according to the ingredients

As a result of the sensory test of the beverage containing the herbal medicine mixed extract according to the compounding material, there was almost no difference in color in Experimental Example 1, Comparative Example 1, and Comparative Example 2, but in Experimental Example 1, the bitter taste was neutralized and the characteristic flavor of the herbal medicine was reduced. Therefore, compared to Comparative Examples 1 and 2, it could be easily ingested, and the overall preference was the most remarkable.

flavor incense color Comprehensive symbol map Experimental Example 1 8.4 7.9 7.4 8.5 Comparative Example 1 5.6 5.5 7.3 5.6 Comparative Example 2 5.3 5.2 7.4 5.1

2) Herbal medicine mixed extract according to the ingredients

As a result of the sensory test of the beverage containing the herbal medicine mixed extract according to the mixing ratio, there was almost no difference in color in Experimental Example 1, Comparative Example 3 and Comparative Example 4, and Experimental Example 1 had less bitter taste than Comparative Examples 3 and 4, and odor It was possible to eat softly because there was no

flavor incense color Comprehensive symbol map Experimental Example 1 8.4 7.9 7.4 8.5 Comparative Example 3 7.8 7.5 7.4 7.5 Comparative Example 4 7.5 7.4 7.3 7.3

In addition, Experimental Example 1 and Experimental Example 2 to which salted sewage was added had no difference in overall preference, and the beverage of Experimental Example 3, in which taurine, guarana extract powder, and fermented honey were mixed, had enhanced sweetness compared to Experimental Example 1. It was easy to ingest, so it had the best taste.

Claims (6)

1) after washing turmeric, jujube, arrowroot, sukjihwang, angelica, hwanggi, cheongung, goji berry, dried ginger, upper extremity, bokryeong, injin mugwort and earth worm, mixing the washed herbal material with water; and
2) hot water extraction of the herbal medicine mixture of step 1), which is effective in improving liver function, including a method for producing a herbal medicine mixture beverage with improved palatability. The method of claim 1, wherein the mixing of step 1) is 0.5 ~ 1.5 kg of turmeric, 0.25 ~ 0.75 kg of jujube, 0.25 ~ 0.75 kg of arrowroot, 0.05 ~ 0.15 kg of shuangi, 0.05 ~ 0.15 kg of angelica, 0.05 ~ 0.15 kg of astragalus per 100 L of water 0.15kg, cheongung 0.05~0.15kg, goji berry 0.1~0.2kg, dry ginger 0.1~0.3kg, upper extremity 0.5~1.5kg, bokryeong 0.05~0.15kg, injin mugwort 0.02~0.07kg, and ginseng 0.25~0.75kg A method of producing a herbal medicine mixed beverage with improved taste and efficacy in improving liver function, characterized in that it is effective in improving liver function. The method according to claim 1, wherein the hot water extraction in step 2) is effective in improving liver function, characterized in that it is extracted at 80 to 120° C. for 3 to 5 hours, and has improved palatability. According to claim 1, wherein the method for preparing the beverage
1) 100L of water containing 5~15% (v/v) saltwater after washing turmeric, jujube, arrowroot, Sukjihwang, angelica, hwanggi, cheongung, gugija, dried ginger, upper extremities, bokryeong, injin mugwort and earth worm. For the above washed turmeric 0.8~1.2kg, jujube 0.4~0.6kg, kudzu 0.4~0.6kg, sukhumang 0.08~0.12kg, angelica 0.08~0.12kg, astragalus 0.08~0.12kg, cheongung 0.08~0.12kg, wolfberry 0.13~ 0.17kg, dry ginger 0.15~0.25kg, upper extremity 0.8~1.2kg, bokryeong 0.08~0.12kg, injin mugwort 0.04~0.06kg, and ginseng 0.4~0.6kg; and
2) extracting the herbal medicine mixture of step 1) with hot water at 90 ~ 110 ℃ for 3 ~ 5 hours; 5. The method of claim 4, wherein after step 2), 0.1 to 0.5 parts by weight of taurine, 0.05 to 0.15 parts by weight of guarana extract powder, and 1.5 to 2.5 parts by weight of specification honey based on 100 parts by weight of the herbal medicine mixed hot water extract. A method of producing a herbal medicine mixed beverage having an effect on improving liver function and having improved palatability, characterized in that by adding a step; [Claim 6] An herbal medicine mixed beverage having an effect on improving liver function and improved palatability, prepared by the method of any one of claims 1 to 5.

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