KR20210062240A - water purification method and system thereof using symbiosis of microalgae and microorganism - Google Patents

Abstract

The present invention relates to a water purification method and a water purification device for removing heavy metals (Cd, As, Zn, Pb, Cu, Cr, Fe) from wastewater by introducing microorganisms (Bacillus sp.) that promote the growth of microalgae. According to the present invention, an effect of continuously removing heavy metals was obtained by using microorganisms that promote the growth of microalgae even in an environment harmful to the growth of microalgae having a high degree of heavy metal contamination. Biomass thus obtained can be utilized as biomass for additional heavy metal treatment.

Description

TECHNICAL FIELD [Water purification method and system thereof using symbiosis of microalgae and microorganism]

Microalgae can control eutrophication by nitrogen and phosphorus through photosynthesis in water and remove heavy metals. On the other hand, microorganisms are known to help the effective growth of microalgae by interacting with microalgae, such as exchanging vitamins and organic substances in the water system, and to remove contamination by association with microalgae in a specific contaminated environment. . However, high concentrations of heavy metals inhibit the growth of microalgae, making it impossible for microalgae to effectively purify the water quality.

In the prior art, purification using living organisms mainly uses a method using adsorption of dead cells to the outside, but this method has a disadvantage in that the amount of cells to be used increases depending on the degree of contamination. In order to overcome these shortcomings, if heavy metals are removed while growing microalgae, continuous treatment is possible without the addition of additional cells, but there are difficulties such as inhibiting growth or killing microalgae in severe cases when contaminated with specific heavy metals. .

In an environment containing high concentrations of heavy metals, microalgae can remove heavy metals and at the same time select microorganisms that effectively grow microalgae, and cultivate these microalgae and microalgae at the same time, so that microalgae do not die and are contaminated with specific heavy metals. There is a need for technology that can effectively handle the environment.

The water purification method of the present invention relates to a method of removing heavy metals (Cd, As, Zn, Pb, Cu, Cr, Fe) from wastewater by introducing microorganisms (Bacillus sp.) that promote the growth of microalgae.

The method of the present invention is characterized in that the microalgae are green algae, and a part of the microalgae whose growth has been promoted is harvested, and the remaining part is reused.

The invention of the present invention is characterized in that the microorganism is at least one of Bacillus sp., Bacillus altitudinis, or Bacillus aryabhattai.

The method of the present invention is characterized in that the microalgae is at least one of Haematococcus, Scenedesmus, Chlorella, Coelastrum, Gloeocystis, or Micractinium.

The method of the present invention is characterized in that the heavy metal is at least one of Cd, As, Zn, Pb, Cu, Cr, or Fe.

The method of the present invention is characterized in that the microalgae is Chlorella, and the heavy metal is at least one of Cd, Zn, Pb, Cu, Cr, or Fe.

The method of the present invention is characterized in that the microalgae is Micractinium, and the heavy metal is any one or more of As, Zn, Pb, or Cu.

The method of the present invention is characterized in that the microalgae is Coelastrum, and the heavy metal is at least one of Cd, Zn, Cu, or Fe.

The method of the present invention is characterized in that the microalgae is Haematococcus.

The method of the present invention is characterized in that the microalgae is Haematococcus, and the heavy metal is at least one of Cd, As, Zn, Pb, Cu, Cr, or Fe.

The method of the present invention is characterized in that the microalgae is Scenedesmus, and the heavy metal is at least one of Cd, Zn, Pb, Cr, or Fe.

The method of the present invention is characterized in that the microalgae is Gloeocystis, and the heavy metal is any one or more of As, Zn, Pb, or Cu.

The method of the present invention is characterized in that the mixing ratio of microorganisms based on 1 g/L of microalgae is 0.1 g/L.

The water purification apparatus of the present invention includes a water tank containing microalgae in which harmful substances including heavy metals promote growth.

The apparatus of the present invention is characterized in that there are two or more water tanks, each tank having a height difference, and having a connection portion between the tanks.

In the device of the present invention, the connection portion is isolated by a semi-permeable membrane.

The water purification apparatus of the present invention includes at least one step 1 microalgal tank for treating harmful substances including heavy metals so that harmful substances containing heavy metals present at a high concentration do not inhibit the growth of a specific microalgae group; and And one or more two-stage microalgae tanks for treating harmful substances including heavy metals so that harmful substances containing heavy metals present do not inhibit the growth of other microalgae except for the specific microalgae; and the microalgae tank of the microalgae tank. The space is separated by a semi-permeable membrane.

The apparatus of the present invention is characterized in that it further comprises one or more three-stage microalgae tanks for treating harmful substances containing heavy metals that do not inhibit the growth of specific microalgae at high concentrations.

In the apparatus of the present invention, the heavy metal of the first stage microalgae tank is at least one of Zn, Cu, or Cd, and at this time, the microalgae are Micractinum sp., Coelastrum sp. Or Haematococcus sp. Any one or more of, and the heavy metal of the second-stage microalgae tank is at least one of pb or Cr, and the microalgae is Scenedesmus sp. Micractinum sp. Or Haematococcus sp. It characterized in that any one or more of.

In the apparatus of the present invention, the harmful substance of the three-stage microalgae tank is at least one of nitrogen or phosphorous, and the microalgae are Chlorella sp., Scenedesmus sp. Or it is characterized in that at least one of Gloeocystis sp.

The device of the present invention is characterized in that the microalgae are cultivated with Bacillus sp. to increase the survival rate even under heavy metal conditions.

According to the present invention, even in a harmful environment having a high degree of heavy metal contamination, the effect of removing heavy metals was obtained by utilizing microorganisms that promote the growth of microalgae. The biomass thus obtained can be used as biomass for additional heavy metal treatment.

Fig. 1. Separation of microorganisms from microalgae communities that grow naturally in artificial lakes in Sejong City
Figure 2. Separation and identification of 7 kinds of microorganisms that coexist with microalgae
Figure 3. Differences in the growth rate of Chlorella by microorganisms mixed with Chlorella
Figure 4. Effluent water from an abandoned mine where microalgae were collected
Fig. 5. Selection and separation of microalgae
Fig. 6. Bacillus sp. And Chlorella sp. Comparison of heavy metal removal efficiency of (standard solution is the first value)
Fig. 7. Growth rate of Chlorella when Bacillus is mixed with Chlorella (microorganisms added)
Fig. 8. Growth rate of Micractinium when Bacillus is mixed with Micractinium (microorganisms added)
Fig. 9. Growth rate of Coelastrum when Bacillus is mixed with Coelastrum (microorganisms added)
Figure 10. Haematococcus growth rate when Bacillus is mixed with Haematococcus (microorganisms added)
Fig. 11. Growth rate of Scenedesmus when Bacillus is mixed with Scenedesmus (microorganisms added)
Fig. 12. Growth rate of Gloeocystis when Bacillus is mixed with Gloeocystis (mi: added microorganism)
Figure 13. Heavy metal residual value by microalgae
14. Image visualizing the reduction of heavy metals by microalgae
Fig. 15. Changes in growth rate according to supply concentration of microorganisms compared to microalgae
Figure 16. Confirmation of growth promotion when microorganisms are mixed under heavy metal-containing conditions
Fig. 17. Terraced water tank simulating an inclined natural waterway
Fig. 18. Example of flow of treated water in individual tanks
Figure 19. Example of arrangement of a heavy metal treatment tank following the flow of treated water
Figure 20. Water flow simulation
Fig. 21. Microalgae and microbial communities remaining in the reactor even at high flow rates

Hereinafter, experiments and examples of the present invention will be described. The scope of the present invention is determined by the claims, and is not limited by the following experiments and examples. Accordingly, these experiments and modifications of the examples belong to the scope of the present invention by the interpretation of the claims of the present invention.

Experiment 1. Selection of microorganisms that promote the growth of microalgae

The applied microalgae were Haematococcus, Scenedesmus, Chlorella, Coelastrum, Gloeocystis, and Micractinium. The initial inoculum of microalgae used for the experiment was cultured for about 7 days at a level of about 0.02 g/L, and the final biomass after cultivation increased from 5 to 15 times at the level of about 0.1 to 0.3 g/L.

The medium was used by mixing 10% OHM medium and 0.5% LB medium in distilled water in order to reproduce the contaminated environment at the level of 10 ppm nitrogen and 1 ppm phosphorus, which are generally polluted concentrations in rivers. OHM (Optimal Haematococcus Medium) medium per 1 L of KNO3 0.41 g, Na2HPO4 0.03 g, MgSO4 7H2O 0.246 g, CaCl2 2H2O 0.11 g and Fe(III) citrate H2O 2.62 mg, CoCl2 6H2O 0.011 mg, CuSO4 5H2O 0.01 mg, Cr2O3 0.075 mg, MnCl2·4H2O 0.98 mg, Na2MoO4·2H2O 0.12 mg, SeO2 0.005 mg and biotin 25 ug, thiamine 17.5 ug, B12 15 ug. It contains 5.0 g of yeast extract. In the experiment to confirm the efficiency of heavy metal treatment, the heavy metals Cd, As, Zn, Pb, Cu, Cr, Fe, which are the target heavy metals, were added to the medium conditions according to the conditions, and the concentrations were all added at a level of 5 ppm. Medium was used.

After classifying and identifying 7 kinds of microorganisms (FIG. 1) that coexist with microalgae, they were mixed with Chlorella, a representative microalgae, and the growth rate was compared (FIG. 3). Bacillus sp. Was found to have the effect of promoting the growth of microalgae, and Bacillus sp. showed the highest growth effect at 151%. All three species that promoted growth were of the genus Bacillus, and Chromobacterium sp. And Chryseobacterium sp. inhibited the growth of microalgae.

Experiment 2. Selection of microalgae effective for heavy metal treatment

The effluent generated from the abandoned mine contains a large amount of heavy metals, and microalgae were collected (FIG. 4) by visiting a nearby river from which it flowed, and then 7 kinds of microalgae were selected and separated (FIG. 5).

Experiment 3. Comparison of microbial removal of heavy metals Bacillus sp. The heavy metal removal efficiency of and Chlorella sp was compared (Fig. 6). In the medium we used, the growth of Bacillus was hardly observed, and the ability to remove heavy metals was hardly observed. Chlorella was able to grow effectively and it was confirmed that most of the heavy metals can be removed. In other words, it is judged that the increase of chlorella (microalgae) biomass plays an important role for the removal of heavy metals in water.

Experiment 4. Comparison of growth rates of 6 types of microalgae selected under various heavy metal conditions (with or without Bacillus microorganisms)

As, Pb, and Fe, as known in the prior art, are the cases where they grow better than the control regardless of whether or not microorganisms are added. What is to be noted in the present invention is an experimental result of an increase in the growth rate of microalgae that are poorly grown in the presence of a specific heavy metal. Referring to the example of FIG. 7, the growth rate is 4% compared to the control in the presence of cadmium (Cd), which is in a state in which growth is not possible. It increased about 2 times from 31% to 64%, about 4 times in the case of chromium (cr) from 12% to 50%, and cadmium (Cd) increased from 4 to 27. In the case of such heavy metals, in the prior art, microalgae dies and cannot be used continuously, but cultivation with microorganisms increases the growth rate, so that continuous cultivation becomes possible.

That is, Bacillus sp. When microorganisms are added (e.g., Cd taste, As taste), it can be confirmed that the biomass growth rate is higher in the Cd, Zn, Pb, Cu, Cr, Fe environment than the case without microorganisms. In particular, it was confirmed that the rate of increase of biomass increased by 2 to 5 times or more under the conditions other than Pb, so in the case of Chlorella, it was confirmed that cultivation with microorganisms increased the growth properties under heavy metal conditions.

Bacillus sp. When microorganisms are added, it can be seen that biomass growth is higher in As, Zn, Pb, Cu, and Fe environments than when there are no microorganisms. In particular, the rate of increase of biomass is the highest in As, Zn, Pb, and Cu environments. In the case of Micractinium sp., it can be confirmed that in the case of Micractinium sp. There was (Fig. 8).

Bacillus sp. When microorganisms are added When added, it can be seen that biomass growth is higher than when there are no microorganisms in Cd, Zn, Cu, and Fe environments. Therefore, in the case of Coelastrum sp, it was confirmed that the microalgae did not die and the growth property was improved by culturing with microorganisms under the condition of the heavy metal addition (FIG. 9).

10 shows the growth rate of Haematococcus (microorganisms added) when Bacillus is mixed with Haematococcus. Haematococcus in Bacillus sp. When microorganisms were added, it was confirmed that the biomass growth was higher in the Cd, As, Zn, Pb, Cu, Cr, Fe environment than when there were no microorganisms when added, and this means that Haematococcus removed much more than other microalgae. It showed the range and increased efficiency. In particular, it shows growth of 130% in the condition of 590% and Zn in the As condition, 390% in the case of Pb, and 140% in the condition of Cr. In the case of Haematococcus sp, it can be confirmed that cultivation with microorganisms under the conditions of the heavy metal prevents the death of microalgae and enhances the growth properties, and it was found to be the most efficient among the isolated seeds.

11 shows the growth rate of Scenedesmus when Bacillus is mixed with Scenedesmus (microorganisms added), and Bacillus sp. It can be seen that the biomass growth rate is higher when microorganisms are added than when microorganisms are not present. Therefore, in the case of Scenedesmus sp, it was confirmed that microalgae cultivated together with microorganisms did not die under the conditions in which the heavy metal was added and showed a high growth rate.

12 shows the growth rate of Gloeocystis when Bacillus is mixed with Gloeocystis (microorganisms added), and Bacillus sp. It can be seen that when microorganisms are added, biomass grows more than when microorganisms are not present. Therefore, in the case of Gloeocystis sp, it was confirmed that the microalgae cultured together with the microorganisms in the condition in which the heavy metal was added did not die and the growth rate was increased. From these experimental results, it is expected that when cultured with green algae and Bacillus, there will be an effect of increasing the growth rate of green algae under conditions containing heavy metals. These results were also confirmed in other microalgae, Coccomyxa sp., Chlorococcum sp., and Gloeothece sp.

Experiment 5. Heavy metal removal efficiency (ICP analysis)

13 shows the residual value of heavy metals by microalgae, and FIG. 14 is an image visualizing the reduction of heavy metals by microalgae. It was confirmed that microalgae effectively remove all heavy metals except arsenic regardless of the use of microorganisms. However, when the wastewater is treated with microalgae for a week, the growth rate is about 5 times higher than that of the initial biomass under heavy metal-free conditions, and in this case, the growth rate decreases in the case of the experimental zone treated with heavy metals (Figs. 7 to 12). At this time, if the growth rate is less than 20% compared to the control, it cannot be considered that the growth of the cells has been achieved at all (when calculated at a level of 20% compared to the five-fold growth in the same period under heavy metal-free conditions, the initial inoculation amount is almost the same). In this case, the cells did not grow and died, and even if the cells died, heavy metals were adsorbed to the dead cells, so that the first one can be removed.However, it is possible to recycle the same cells treated with heavy metals and continuously treat them. It is difficult because it cannot be attached anymore Therefore, raising the growth rate to a level of at least 40% or more under heavy metal conditions is a necessary process for continuous treatment (50% recovery and 50% reuse), Bacillus sp. These results can be achieved by using the spawn. Therefore, it is possible to recover the grown biomass and treat heavy metals again.

Experiment 6. Supply concentration of microorganisms that have an effect on the growth of microalgae

In order to confirm the optimal supply ratio of microalgae to microalgae, 0, 5, 40, and 200ul of microbes were supplied respectively to the inoculation amount of 0.02g/L of Chlorella microalgae (0, 0.0125, 0.1, 0.5 times the weight of microalgae inoculation, respectively. Microalgae 1g/L standard microalgae 0.1g/L standard when supplying 40ul microbial inoculum level). As a result of the experiment, As for the 5 ul supply test group and Fe at the 200 ul supply test group, the highest growth rate was shown in all other heavy metals at 40 ul (FIG. 15).

Experiment 7. Re-inoculation of produced microalgal biomass

When heavy metals are removed using microalgae, most of the microalgae die under heavy metal conditions, making it difficult to reuse due to removal by simple adsorption. It was confirmed that microalgae cultivated with microorganisms proceeded with gentle growth even under these adverse conditions.

16 shows that growth is promoted when microorganisms are mixed and used under heavy metal-containing conditions. The condition inside the red square in the lower right is a condition that contains microorganisms, and the condition in the lower left is the condition that it hardly grows without microorganisms, so there is no color. The condition shown above is a control without heavy metals, and it can be seen that when microorganisms are contained, it grows as well as the control.

This water purification method was implemented in a water purification device. The water purification apparatus of the present invention includes a water tank containing microalgae in which harmful substances including heavy metals promote growth. In such a water purification apparatus, there are two or more water tanks, each of which has a height difference, and may have a connection part between the water tanks. In addition, the connection portion may be isolated by a semi-permeable membrane.

An embodiment of the water purification apparatus of the present invention includes three or more water tanks including a water tank containing microalgae for promoting growth of harmful substances including heavy metals; A height difference formed between each of the water tanks; and a connection part formed in the adjacent water irradiation tooth, wherein each connection part is installed in a form that does not face the other connection part, so that the water flow is stagnant in each of the water tanks. It is characterized in that it induces the point where the microalgae does not float but sinks and stays.

In the water purification apparatus of the present invention, a medium with a rough surface (for example, pulp or nonwoven fabric) that helps the attachment of microalgae to the point where the water flow is stagnant can be installed to induce the microalgae to gather without floating. 17 shows a staircase-shaped waterway made by simulating a natural waterway with a slope. The semi-permeable membrane shown in FIG. 17 induces eddy current by installing (zigzag) the outlet through which the treated water flows at a distance from each other in a diagonal to ensure the minimum residence time of the treated water in each tank. The permeable membrane 170 was disposed so that the microalgal cells were not mixed with the water tank on the downstream side. Such a semi-permeable membrane can give various changes to the installation area and installation location (174, 175).

In a place (171, 172, 173 in the water tank) where the fluid flow shown in FIG. 18 flows down along with the treated water and becomes stagnant (in the water tank 171, 172, 173), microalgae that are effective for treating different heavy metals can be disposed for each location. Alternatively, as shown in FIG. 19, a treatment system in the form of setting a treatment zone for microalgae to pass through according to the type of heavy metal contained in the treated water may be made. However, depending on the microalgae, heavy metals whose growth is inhibited in most types can be placed as a preferential treatment zone. For example, in the case of treated water containing Fe, Pb, and Zn, in the first step, the Micractinium zone, which has a high survival rate in Zn, is first passed, and then the Haematococcus seed zone, which has high treatment efficiency and survival rate of Cr, Pb, is passed. And finally, the Scenedesmus seed zone, which treats low-risk Fe, or the Chlorella zone, is called to remove residual nitrogen and phosphorus. At this time, the residence time may vary depending on the condition of the treated water and the treatment area from as little as several minutes to as long as 10 days or more.

The experimental results of the water purification method using the apparatus of FIG. 19 are expressed as examples as follows. An embodiment of the water purification apparatus of the present invention is to treat the toxic substances containing the heavy metals so that the toxic substances containing one or more heavy metals among Cd, Zn, or Cu present in a high concentration do not inhibit the growth of one or more microalgae. At least one step 1 microalgal tank; and at least one second step of treating the hazardous substances containing the heavy metals so that the hazardous substances containing at least one heavy metal among Cr or Pb present in a high concentration do not inhibit the growth of at least one microalgae And a microalgal tank, and the microalgal tank is separated by a semi-permeable membrane.

The microalgae is any one or more of Haematococcus, Scenedesmus, Chlorella, Coelastrum, Gloeocystis, or Micractinium, and all of these 6 species have a survival rate of 40% or less under Cd conditions, a survival rate of 42% or less under Zn conditions, and 25 under Cu conditions. The heavy metal under conditions that inhibit the growth of all 6 species of microalgae used in the experiment in% or less is Cd, Zn, or Cu. Among the six microalgae used in the experiment, the survival rate of Haematococcus (80%), Scenedesmus (71%), Chlorella (12%), Gloeocystis (79%), and Coelastrum (70%) under the Cr condition was inhibited. , Gloeocystis (11%) is a heavy metal under conditions that inhibit the growth of four species of microalgae by inhibiting the survival rate of Cr or Pb.

The water purification apparatus of the present invention may further include one or more three-stage microalgae tanks for treating harmful substances including heavy metals that do not inhibit the growth of specific microalgae at high concentrations.

In an embodiment of the water purification apparatus of the present invention, the heavy metal of the first-stage microalgae tank is at least one of Zn, Cu, or Cd, and at this time, the microalgae are Micractinum sp., Coelastrum sp. Or Haematococcus sp. It characterized in that any one or more of.

In an embodiment of the water purification apparatus of the present invention, the heavy metal of the two-stage microalgae tank is at least one of pb or Cr, and the microalgae are Scenedesmus sp., Micractinum sp. Or Haematococcus sp. It characterized in that any one or more of.

In an embodiment of the water purification apparatus of the present invention, the harmful substance of the three-stage microalgae tank is at least one of nitrogen or phosphorous, and the microalgae are Chlorella sp., Scenedesmus sp. Or Gloeothece sp. It characterized in that any one or more of.

In an embodiment of the water purification apparatus of the present invention, the microalgae are cultured with Bacillus sp., so that the survival rate is increased even under heavy metal conditions.

FIG. 20 is an image that simulates the process of flowing down the treated water along the stepped water tank, showing the flow of water (green) and the vortex formation point and the stagnation point (dark blue) where the flow rate is relatively high. .

FIG. 21 shows a state in which microalgae and microbial communities gathered at the bottom, which are floating at the vortex point formed when the water purification device of the present invention is operated and water flows in the water tank, do not flow along the water current, but appear in the process of flowing water. This is consistent with the result of showing a stagnant water flow according to the formation of a vortex in the simulation of FIG. 20. By forming a vortex in this way, the microalgae and microbial communities are prevented from being swept to the outside of the water tank of the water purification device, and at the same time, the efficiency of the water purification treatment is enhanced by fixing it at the intended destination point.

Although the above description has been made focusing on experiments and examples, these are only examples and do not limit the present invention, and those of ordinary skill in the art to which the present invention belongs are not departing from the essential characteristics of the experiment and the examples above. It will be appreciated that various modifications and applications that have not been tested or illustrated are possible. That is, since each component specifically shown in the experiment and the examples can be modified and implemented, differences related to such modifications and applications should be construed as being included in the scope of the present invention defined in the appended claims.

170 Zigzag installed semi-permeable membrane
171 Tank 1
172 Tank 2
173 Fish Tank 3
174 Where the semi-permeable membrane can be formed 1
175 Where a semi-permeable membrane can be formed 2
176 Fluid Flow
177 Congestion Area
190 Example of arrangement standard for heavy metal treatment tank
191 Heavy metal treatment zone that inhibits the growth of many microalgae in high concentration cities
192 Heavy metals that inhibit the growth of specific microalgae in high concentrations
193 Heavy metals that do not inhibit the growth of most microalgae even at high concentrations
194 Processing order
195 Example of arrangement and flow direction of treatment tanks for each heavy metal
Simulated image of 200 cascading tanks viewed from the side
201 Vortex or congestion point 1
202 Vortex or congestion point 2
203 Semi-permeable membrane installation location
Simulated image of 204 cascading tank from above

Claims (21)

A water purification method that removes heavy metals from wastewater by introducing microorganisms that promote the growth of microalgae. The method of claim 1,
The microalgae are green algae,
A water purification method for continuously removing heavy metals in water, characterized in that a part of the microalgae whose growth has been promoted is harvested and the remaining part is reused. The method of claim 1,
The water purification method, characterized in that the microorganism is at least one of Bacillus sp., Bacillus altitudinis, or Bacillus aryabhattai. The method of claim 1,
The water purification method, characterized in that the microalgae is at least one of Haematococcus, Scenedesmus, Chlorella, Coelastrum, Gloeocystis, or Micractinium. The method of claim 1,
Water purification method, characterized in that the heavy metal is at least one of Cd, As, Zn, Pb, Cu, Cr, or Fe. The method of claim 1,
The microalgae is Chlorella, and the heavy metal is Cd, Zn, Pb, Cu, Cr, or a water purification method, characterized in that at least one of Fe. The method of claim 1,
The water purification method, characterized in that the microalgae is Micractinium, and the heavy metal is any one or more of As, Zn, Pb, or Cu. The method of claim 1,
The water purification method, characterized in that the microalgae is Coelastrum, and the heavy metal is at least one of Cd, Zn, Cu, or Fe. The method of claim 1,
Water purification method, characterized in that the microalgae is Haematococcus. The method of claim 1,
The water purification method, characterized in that the microalgae is Haematococcus, and the heavy metal is at least one of Cd, As, Zn, Pb, Cu, Cr, or Fe. The method of claim 1,
The microalgae is Scenedesmus, and the heavy metal is Cd, Zn, Pb, Cr, or a water purification method, characterized in that at least one of Fe. The method of claim 1,
The microalgae is Gloeocystis, and the heavy metal is any one or more of As, Zn, Pb, or Cu. The method of claim 1,
The microalgae is Gloeocystis, and the heavy metal is any one or more of As, Zn, Pb, or Cu. A water purification device including a tank containing microalgae that promotes the growth of harmful substances including heavy metals. The method of claim 14,
There are two or more of the above tanks,
Each tank has a height difference,
Water purification apparatus, characterized in that having a connection between the water tank. The method of claim 15,
The water purification apparatus, characterized in that the connection portion is isolated by a semi-permeable membrane. One or more first-stage microalgae tanks for treating harmful substances containing heavy metals so that harmful substances containing heavy metals present at a high concentration do not inhibit the growth of specific microalgae groups; And
And one or more two-stage microalgae tanks for treating harmful substances containing heavy metals so that harmful substances containing heavy metals present at a high concentration do not inhibit the growth of other microalgae except for the specific microalgae; and
A water purification device separated by a semi-permeable membrane between the microalgae tanks. The method of claim 17,
One or more three-stage microalgae tanks for treating harmful substances containing heavy metals that do not inhibit the growth of specific microalgae in high concentrations; water purification apparatus further comprising. The method of claim 17,
The heavy metal of the first stage microalgae tank is one or more of Zn, Cu, or Cd, and at this time, depending on the type of heavy metal, the microalgae are Micractinum sp., Coelastrum sp. Or any one or more of Haematococcus sp.,
A water purification apparatus, characterized in that the heavy metal of the second-stage microalgae tank is at least one of pb or Cr, and the microalgae is at least one of Scenedesmus sp., Micractinum sp., or Haematococcus sp. The method of claim 18,
The toxic substance in the three-step microalgae tank is at least one of nitrogen or phosphorous, and the microalgae are Chlorella sp., Scenedesmus sp. Or Gloeocystis sp. Water purification device, characterized in that at least one of. The method of claim 17,
The microalgae are cultured with Bacillus sp., so that the survival rate is increased even under heavy metal conditions.

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