KR20210058031A - Cosmetic composition for improving skin moisture comprising a hydrolyzed rice protein and process for preparing thereof - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving skin moisture containing hydrolyzed rice protein and to a manufacturing method thereof, and more particularly, to a cosmetic composition having an effect of improving skin moisture by extracting the hydrolyzed rice protein from rice and the manufacturing method thereof, wherein the cosmetic composition according to the present invention significantly improves moisture retention without irritation to the skin, and also the manufacturing method according to the present invention provides an optimal manufacturing process for the cosmetic composition containing the rice protein.

Description

A cosmetic composition for improving skin moisture comprising a hydrolyzed rice protein and a method for preparing the same

The present invention relates to a cosmetic composition comprising a hydrolyzed rice protein and a method for producing the same, and more particularly, to a cosmetic composition having an effect of improving skin moisture without irritation by extracting hydrolyzed rice protein from rice, and a method for manufacturing the same. About.

Rice protein extracted from rice has a relatively low protein content, but quantitative and qualitative interest in rice protein is again being focused as a high-quality protein source.In particular, rice contains about 7% protein. However, it has a digestibility of more than 90% compared to other cereal proteins, and at the same time, it has a high content of lysine, an essential amino acid, and is known as an excellent protein source because it has a balanced amino acid composition.

In addition,'rice bran' is a Chinese character of rice bran, which refers to a pulverized mixture of peel, seed skin, and algae layer, etc., which are formed in the process of making polished rice by grinding brown rice. The weight ratio of rice bran to brown rice before grinding is Although only 10%, rice bran contains rich minerals, nutrients, and functional ingredients equivalent to about 75% of the total rice. In particular, rice bran contains a large amount of dietary fiber, vegetable protein, and other useful ingredients, so it has been reported to have excellent blood cholesterol lowering effect, antioxidant effect, and blood pressure increase inhibitory effect (Nicolsi et al., 1993; Capro et al., 1997). ), tocopherol, phytic acid, phenolic acid, oryzanol, ferulic acid, etc., which are excellent in physiological activities such as antioxidant power, were reported. (Andreason et al., 1999; Kikuzaki et al., 2002).

In spite of the excellent functional effects of rice as a food, the frequency of use of rice was very insufficient in the cosmetics industry. This not only causes discoloration and discoloration when applying the protein material obtained from the existing rice bran to cosmetics, but also various active ingredients (gamma-orizanol, octacose, ceramide, etc.) are removed during the protein purification step, thereby enhancing the functionality as a cosmetic ingredient. There is a problem to be lost.

In addition, as a commonly used method of purifying rice protein, there is a method of separating a mixture of rice protein and starch by immersing it in a large amount of sodium hydroxide solution for several hours. This is a by-product that produces large amounts of salts and harmful substances. There was a problem.

Accordingly, as a result of continuous research on the purification of rice protein for use as a cosmetic composition, the raw materials and enzymes used in the process were specified in the process of purifying rice protein using glycolytic enzymes and proteolytic enzymes after crushing rice. Then, the present invention was completed by optimizing the purification conditions.

In order to solve the conventional problems as described above, the present invention provides a cosmetic composition having an effect of improving skin moisture without irritation by extracting hydrolyzed rice protein from rice, and an optimal method for extracting hydrolyzed rice protein from rice. Having that purpose.

In order to achieve the above object, the present invention provides a cosmetic composition for improving skin moisture containing hydrolyzed rice protein.

In the present invention, the cosmetic composition is based on a total of 100% by weight of the total cosmetic composition, glycerin (Glycerin) 10 to 20% by weight, butylene glycol (Butylene Glycol) 4 to 6% by weight, squalane (squalane) 1 to 3% by weight, 1 to 3% by weight of niacinamide, 1 to 3% by weight of Hydrolyzed Rice Protein, 0.5 to 1.5% by weight of Octyldodecyl Myristate, 1,2- Hexanediol (1,2-Hexanediol) 0.5 to 1.5% by weight, betaine (betaine) 0.6 to 1% by weight, lanoline 0.4 to 0.6% by weight, pentaerythrityl tetraethylhexanoate (Pentaerythrityl Tetrathylhexanoate) 0.4 To 0.6 wt%, Cyclohexasiloxane 0.4 to 0.6 wt%, Dimethicone 0.4 to 0.6 wt%, Cyclopentasiloxane 0.4 wt%, Stearic Acid 0.2 to 0.4 wt%, PEG-100 Stearate 0.2 to 0.4 wt%, Caprylyl Glycol 0.2 to 0.4 wt%, Stearyl alcohol 0.1 to 0.3 wt%, Cetyl Alcohol (Cetyl Alcohol) 0.1 to 0.3 wt%, Glyceryl Stearate 0.1 to 0.3 wt%, Tromethamine 0.05 to 0.15 wt%, Carbomer 0.05 to 0.15 wt%, Perfume 0.05 to 0.15 wt%, Xanthan Gum 0.05 to 0.15 wt%, Polysorbate 20 0.05 to 0.15 wt%, Disod uim EDTA) It may be characterized in that it consists of 0.02 to 0.04% by weight and purified water purified water.

More preferably, the cosmetic composition is based on a total of 100% by weight of the total cosmetic composition, glycerin (Glycerin) 15% by weight, butylene glycol (Butylene Glycol) 5% by weight, squalane (squalane) 2% by weight, niacinamide (Niacinamide) 2% by weight, Hydrolyzed Rice Protein 2% by weight, Octyldodecyl Myristate 1% by weight, 1,2-hexanediol (1,2-Hexanediol) 1% %, betaine 0.8% by weight, lanoline 0.5% by weight, pentaerythrityl tetraethylhexanoate 0.5% by weight, cyclohexasiloxane 0.5% by weight, dimethicone ( Dimethicone) 0.5% by weight, Cyclopentasiloxane 0.4% by weight, Stearic Acid 0.3% by weight, PEG-100 Stearate 0.3% by weight, Caprylyl Glycol ( Caprylyl Glycol) 0.3% by weight, Stearyl alcohol 0.2% by weight, Cetyl Alcohol 0.2% by weight, Glyceryl Stearate 0.2% by weight, Tromethamine 0.1% by weight, Carbomer 0.1% by weight, fragrance 0.1% by weight, Xanthan Gum 0.1% by weight, Polysorbate 20 0.1% by weight, Disodium EDTA 0.03% by weight and purified water It may be characterized in that it is composed of purified water.

In the present invention, the cosmetic composition may be characterized in that the essence (essence) formulation.

In the present invention, the cosmetic composition is based on a total of 100% by weight of the total cosmetic composition, glycerin (Glycerin) 8 to 12% by weight, hydrogenated polydecene (Hydrogenated Polydecene) 4 to 6% by weight, cetearyl alcohol ( Cetearyl Alcohol) 2 to 4 wt%, Pentaerythrityladipate/Caprate/Caprylate/Heptanoate 2 to 4 wt%, Hydrolyzed Rice Protein 1 to 3% by weight, propanediol 0.5 to 1.5% by weight, 1,2-hexanediol (1,2-Hexanediol) 0.5 to 1.5% by weight, ethanol (Ethanol) 0.4 to 0.6% by weight, glyceryl Glyceryl Stearate SE 0.4 to 0.6 wt%, Myristyl Alcohol 0.2 to 0.4 wt%, Myristyl Glucoside 0.1 to 0.3 wt%, Butylene Glycol 0.1 to 0.3% by weight, 0.1 to 0.3% by weight of Ceteaeth-20, 0.1 to 0.3% by weight of dimethicone, 0.05 to 0.15% by weight of tocopheryl acetate, acrylate/C10-30 Alkyl acrylate crosspolymer (Acrylates/C10-30 Alkyl Acrylate Crosspolymer) 0.05 to 0.15% by weight, fragrance 0.05 to 0.15% by weight, tromethamine (Tromethamine) 0.05 to 0.15% by weight, and purified water. .

More preferably, the cosmetic composition is based on 100% by weight of the total cosmetic composition, glycerin (Glycerin) 10% by weight, hydrogenated polydecene (Hydrogenated Polydecene) 5% by weight, cetearyl alcohol (Cetearyl Alcohol) 3 Weight%, pentaerythrityladipate/caprate/caprylate/heptanoate (Pentaerythrityladipate/Caprate/Caprylate/Heptanoate) 2.5% by weight, Hydrolyzed Rice Protein 2% by weight, propanediol ( Propanediol) 1% by weight, 1,2-hexanediol (1,2-Hexanediol) 1% by weight, ethanol (Ethanol) 0.5% by weight, Glyceryl Stearate SE 0.5% by weight, myristyl alcohol ( Myristyl Alcohol) 0.3% by weight, Myristyl Glucoside 0.2% by weight, Butylene Glycol 0.2% by weight, Ceteaeth-20 0.2% by weight, Dimethicone 0.2 Wt%, tocopheryl acetate 0.1 wt%, acrylate/C10-30 alkyl acrylate crosspolymer (Acrylates/C10-30 Alkyl Acrylate Crosspolymer) 0.1 wt%, fragrance 0.1 wt%, tromethamine It may be characterized in that consisting of 0.1% by weight and purified water.

In the present invention, the cosmetic composition may be characterized in that the lotion (lotion) formulation.

In order to achieve the above object, the present invention also includes the steps of: S1) adding purified water after pulverizing rice and heating it to 45°C; S2) reacting a reaction solution in which 1% by weight of glycolytic enzyme is mixed with respect to 100% by weight of the total rice for 5 hours; S3) treating the reaction solution at 85 to 95°C for 30 minutes to deactivate the glycolytic enzyme; S4) cooling the reaction solution to 45°C and mixing it with 0.1N aqueous sodium hydroxide solution to adjust the pH to 7; S5) adding a protease to the reaction solution to proceed with the enzyme reaction at 45° C. for 5 hours; S6) treating the reaction solution at 85 to 95°C for 30 minutes to deactivate the protease; S7) cooling the reaction solution to 30 ℃ and extracting the rice protein using 30% ethanol for 48 hours; S8) filtering the extracted rice protein (0.45um~3um) and concentrating at 65~75℃; S9) adding a solvent (1,3-butylene glycol 30%) to the concentrated rice protein and then filtering (0.2um ~ 0.45um); providing a method for producing a hydrolyzed rice protein prepared through do.

In the present invention, the rice may be characterized in that one or more selected from large-eye brown rice, nokchal brown rice, hyangchal brown rice, black rice, or hongchal brown rice.

In the present invention, the glycolytic enzyme may be characterized in that the yeast purification enzyme.

In the present invention, the protease may be characterized in that at least one of Alcalase 2.4L, Bromelain 1200 GDU, or Papain.

The present invention relates to a cosmetic composition comprising a hydrolyzed rice protein and a method for manufacturing the same, and the cosmetic composition according to the present invention not only significantly improves the moisture potency without irritation to the skin, the manufacturing method according to the present invention is It provides an optimal manufacturing process for cosmetic compositions containing rice protein.

1 shows an overall schematic diagram of a manufacturing process of a cosmetic raw material containing rice protein.
2 shows a comparison of the yield of solids and protein according to the hydrolysis ratio (purified water: rice) in order to set the optimal amount of purified water to be added.
3 shows changes in protein content when yeast purification enzyme or BAN 480L is added as a glycolytic enzyme.
Figure 4 shows the comparison of the change in protein content according to the reaction time during the protein enzyme reaction (Alcalase, Bromelain, Papain).
Figure 5 shows the difference in the content of solids and protein according to the amount of ethanol used.
6 shows the difference in protein content extracted according to the raw material (type of rice).
7 shows a comparison of DPPH radical scavenging ability according to raw materials (types of rice).
Figure 8 shows the comparison of ABST radical scavenging ability according to the raw material (type of rice).

The present invention provides a cosmetic composition for improving skin moisture containing hydrolyzed rice protein.

In the present invention, the cosmetic composition is based on a total of 100% by weight of the total cosmetic composition, glycerin (Glycerin) 10 to 20% by weight, butylene glycol (Butylene Glycol) 4 to 6% by weight, squalane (squalane) 1 to 3% by weight, 1 to 3% by weight of niacinamide, 1 to 3% by weight of Hydrolyzed Rice Protein, 0.5 to 1.5% by weight of Octyldodecyl Myristate, 1,2- Hexanediol (1,2-Hexanediol) 0.5 to 1.5% by weight, betaine (betaine) 0.6 to 1% by weight, lanoline 0.4 to 0.6% by weight, pentaerythrityl tetraethylhexanoate (Pentaerythrityl Tetrathylhexanoate) 0.4 To 0.6 wt%, Cyclohexasiloxane 0.4 to 0.6 wt%, Dimethicone 0.4 to 0.6 wt%, Cyclopentasiloxane 0.4 wt%, Stearic Acid 0.2 to 0.4 wt%, PEG-100 Stearate 0.2 to 0.4 wt%, Caprylyl Glycol 0.2 to 0.4 wt%, Stearyl alcohol 0.1 to 0.3 wt%, Cetyl Alcohol (Cetyl Alcohol) 0.1 to 0.3 wt%, Glyceryl Stearate 0.1 to 0.3 wt%, Tromethamine 0.05 to 0.15 wt%, Carbomer 0.05 to 0.15 wt%, Perfume 0.05 to 0.15 wt%, Xanthan Gum 0.05 to 0.15 wt%, Polysorbate 20 0.05 to 0.15 wt%, Disod uim EDTA) It may be characterized in that it consists of 0.02 to 0.04% by weight and purified water purified water.

More preferably, the cosmetic composition is based on a total of 100% by weight of the total cosmetic composition, glycerin (Glycerin) 15% by weight, butylene glycol (Butylene Glycol) 5% by weight, squalane (squalane) 2% by weight, niacinamide (Niacinamide) 2% by weight, Hydrolyzed Rice Protein 2% by weight, Octyldodecyl Myristate 1% by weight, 1,2-hexanediol (1,2-Hexanediol) 1% %, betaine 0.8% by weight, lanoline 0.5% by weight, pentaerythrityl tetraethylhexanoate 0.5% by weight, cyclohexasiloxane 0.5% by weight, dimethicone ( Dimethicone) 0.5% by weight, Cyclopentasiloxane 0.4% by weight, Stearic Acid 0.3% by weight, PEG-100 Stearate 0.3% by weight, Caprylyl Glycol ( Caprylyl Glycol) 0.3% by weight, Stearyl alcohol 0.2% by weight, Cetyl Alcohol 0.2% by weight, Glyceryl Stearate 0.2% by weight, Tromethamine 0.1% by weight, Carbomer 0.1% by weight, fragrance 0.1% by weight, Xanthan Gum 0.1% by weight, Polysorbate 20 0.1% by weight, Disodium EDTA 0.03% by weight and purified water It may be characterized in that it is composed of purified water.

In the present invention, the cosmetic composition may be characterized in that the essence (essence) formulation.

The cosmetic composition is based on a total of 100% by weight of the total cosmetic composition, glycerin (Glycerin) 8 to 12% by weight, hydrogenated polydecene (Hydrogenated Polydecene) 4 to 6% by weight, cetearyl alcohol (Cetearyl Alcohol) 2 to 4 wt%, Pentaerythrityladipate/Caprate/Caprylate/Heptanoate 2 to 4 wt%, Hydrolyzed Rice Protein 1 to 3 wt% , Propanediol 0.5 to 1.5% by weight, 1,2-hexanediol (1,2-Hexanediol) 0.5 to 1.5% by weight, ethanol (Ethanol) 0.4 to 0.6% by weight, glyceryl stearate SE (Glyceryl Stearate SE) 0.4 to 0.6 wt%, Myristyl Alcohol 0.2 to 0.4 wt%, Myristyl Glucoside 0.1 to 0.3 wt%, Butylene Glycol 0.1 to 0.3 wt%, Cete Ares-20 (Ceteareth-20) 0.1 to 0.3% by weight, dimethicone 0.1 to 0.3% by weight, tocopheryl acetate 0.05 to 0.15% by weight, acrylate/C10-30 alkyl acrylate crosspolymer (Acrylates/C10-30 Alkyl Acrylate Crosspolymer) 0.05 to 0.15% by weight, fragrance 0.05 to 0.15% by weight, tromethamine 0.05 to 0.15% by weight, and purified water.

More preferably, the cosmetic composition is based on 100% by weight of the total cosmetic composition, glycerin (Glycerin) 10% by weight, hydrogenated polydecene (Hydrogenated Polydecene) 5% by weight, cetearyl alcohol (Cetearyl Alcohol) 3 Weight%, pentaerythrityladipate/caprate/caprylate/heptanoate (Pentaerythrityladipate/Caprate/Caprylate/Heptanoate) 2.5% by weight, Hydrolyzed Rice Protein 2% by weight, propanediol ( Propanediol) 1% by weight, 1,2-hexanediol (1,2-Hexanediol) 1% by weight, ethanol (Ethanol) 0.5% by weight, Glyceryl Stearate SE 0.5% by weight, myristyl alcohol ( Myristyl Alcohol) 0.3% by weight, Myristyl Glucoside 0.2% by weight, Butylene Glycol 0.2% by weight, Ceteaeth-20 0.2% by weight, Dimethicone 0.2 Wt%, tocopheryl acetate 0.1 wt%, acrylate/C10-30 alkyl acrylate crosspolymer (Acrylates/C10-30 Alkyl Acrylate Crosspolymer) 0.1 wt%, fragrance 0.1 wt%, tromethamine It may be characterized in that consisting of 0.1% by weight and purified water.

In the present invention, the cosmetic composition may be characterized in that the lotion (lotion) formulation.

In order to achieve the above object, the present invention also includes the steps of: S1) adding purified water after pulverizing rice and heating it to 45°C; S2) reacting a reaction solution in which 1% by weight of glycolytic enzyme is mixed with respect to 100% by weight of the total rice for 5 hours; S3) treating the reaction solution at 85 to 95°C for 30 minutes to deactivate the glycolytic enzyme; S4) cooling the reaction solution to 45°C and mixing it with 0.1N aqueous sodium hydroxide solution to adjust the pH to 7; S5) adding a protease to the reaction solution to proceed with the enzyme reaction at 45° C. for 5 hours; S6) treating the reaction solution at 85 to 95°C for 30 minutes to deactivate the protease; S7) cooling the reaction solution to 30 ℃ and extracting the rice protein using 30% ethanol for 48 hours; S8) filtering the extracted rice protein (0.45um~3um) and concentrating at 65~75℃; S9) adding a solvent (1,3-butylene glycol 30%) to the concentrated rice protein and then filtering (0.2um ~ 0.45um); providing a method for producing a hydrolyzed rice protein prepared through do.

In the present invention, the rice may be characterized in that one or more selected from large-eye brown rice, nokchal brown rice, hyangchal brown rice, black rice, or hongchal brown rice.

In the present invention, the glycolytic enzyme may be characterized in that the yeast purification enzyme. Nuruk refers to a fermenting agent made by breeding fungi with enzymes to make alcohol on cereals. Enzymes purified from koji act on both α-1,4 and α-1,6 bonds of starch to break down starch into glucose. It will function.

In the present invention, the protease may be characterized in that at least one of Alcalase 2.4L, Bromelain 1200 GDU, or Papain.

Hereinafter, the present invention will be described in more detail through examples. These examples are for illustrative purposes only, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not construed as being limited by these examples.

Example 1. Preparation of cosmetic ingredients containing rice protein

The overall schematic diagram of the manufacturing process of the cosmetic raw material containing rice protein according to the present invention is shown in FIG. 1, and the specific manufacturing process of the cosmetic raw material containing rice protein is as follows.

1-1. Mixing step of rice and purified water

First, after the rice was pulverized, purified water was added and heated to 45°C. In order to set the optimal amount of purified water added at this time, the yield of solids and protein according to the hydrolysis ratio (purified water: rice) was compared and shown in FIG. 2.

According to FIG. 2, the yield ratio (purified water: rice) increased rapidly from 5:1 to 10:1, but there was no significant difference in the yield from 10:1 to 40:1. Accordingly, the optimum water ratio (purified water: rice) was determined to be 10:1 in consideration of the increase in equipment due to the increase in purified water.

1-2. Glycolytic enzyme treatment step

As a glycolytic enzyme, yeast purification enzyme (SK-Bioland, Korea) or BAN 480L (Novozymes, Denmark) was added and the reaction was carried out at 45° C. for 5 hours, and the results are shown in FIG. 3.

According to FIG. 3, the highest protein extraction amount was shown in the sample using 1.0% yeast purification enzyme, and the protein content of rice generally known as about 68.5mg/ml in terms of the extracted protein content compared to the used rice is 7-8%. You can see that most of them were extracted.

1-3. Glycolytic enzyme inactivation stage

In order to lose the function of the protein enzyme, it was treated for 30 minutes at 85-95°C. Thereafter, it was cooled to 45° C., and the pH was adjusted to 7 using 0.1N aqueous sodium hydroxide solution.

1-4. Protease treatment step

Enzymes used for protein degradation in the present invention are shown in Table 1 below.

sample Protease manufacturer Condition A No treatment - - B Alcalase 2.4L Novozymes, Denmark 55-70℃, pH6.5-8.5 C Bromelain 1200 GDU Novozymes, Denmark 45-50℃, pH6.0-8.0 D Papain Novozymes, Denmark 50-70℃, pH5.0-7.5

The effect of the proteolytic enzyme was analyzed for protein content using a TaKaRa BCA Protein Assay Kit (TAKARA, Japan) after 0.45 μm filtration of the enzyme reaction solution obtained after the reaction for a certain period of time, and the results are shown in FIG. 4.

According to FIG. 4, the protein content of Alcalase, Bromelain, and Papain was higher in the order of 22 hours of enzymatic reaction, and when Alcalase was used, the protein content (4,380 μg/ml) was 2.54 than that of the untreated group (1,720 μg/ml). It appeared twice as high.

As each reaction solution passed 7-8 hours and an odor was generated, the subsequent experiments were carried out by setting the protein enzyme treatment time to 5 hours.

1-5. Step of inactivation of protease

In order to lose the function of the protein enzyme, it was treated for 30 minutes at 85-95°C. After that, it was cooled to 30°C.

1-6. Protein extraction step using ethanol

After deactivating the protease, a certain amount of ethanol was added and immersed for 48 hours, followed by 0.45 μm filtration. At this time, in order to determine the optimal ethanol concentration, solid content and protein content were measured for 0%, 15%, 30%, 50%, and 70% ethanol, respectively, and are shown in FIG. 5.

5, 15% ethanol extract was the highest in solid content, and 70% ethanol extract was the highest in protein. However, when a cosmetic formulation was prepared by adding 30% 1,3-butylene glycol, the samples prepared by extracting 15%, 50%, and 70% ethanol were observed to be suspended/precipitated (Fig. 5, upper right. Reference), the optimal ethanol concentration was determined to be 30%.

1-7. Cosmetic formulation manufacturing step

The extracted protein was filtered (0.45um~3um), concentrated at 65~75℃, and a solvent (30% 1,3-butylene glycol) was added to the concentrated protein, and then filtered again (0.2um~0.45um). Cosmetic raw materials containing rice protein were prepared.

Example 2. Comparison of functional effects according to raw materials

2-1. Comparison of protein content according to raw materials

In the case of using large-eyed brown rice, nokchal brown rice, flavored brown rice, black rice, hongchal brown rice (map rice), and hongchal brown rice (glutinous rice) as raw materials, the protein content is shown in FIG.

According to FIG. 6, the protein content extracted from black rice was the highest, followed by hongchal brown rice (mapped rice) and hyangchal brown rice in that order.

2-2. Comparison of antioxidant effects according to raw materials-DPPH radical scavenging ability

DPPH radical scavenging activity was measured by modifying the method of Shimada K (Shimada K, Fujikawa K, Yahara K, et al. Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J Agric Food Chem 1992;40:945- 948).

DPPH (1,1-diphenyl-2-picrylhydrazyl, MW 394.32g) of 3.94 mg, 0.45 ml of DPPH solution (about 0.1 Mm) in 100 ml of 100% methanol and each sample diluted to a certain concentration in the reaction solution Was mixed in 0.55 ml to conduct an experiment. In the experiment, the antioxidant effect was analyzed by measuring the absorbance at 517 nm with a UV/visible spectrophotometer after allowing it to stand at 37°C for 10 minutes.

The control was measured by the same method by adding a solvent instead of the sample, and methanol was added instead of 0.1Mm DPPH to correct the absorbance value for the color of the sample itself.

DPPH radical scavenging activity was calculated as shown in Equation 1 below, and the results are shown in FIG. 7, and it can be seen that the antioxidant effect of black rice is most excellent at all concentrations.

[Equation 1]

DPPH radical scavenging activity(%) = (1-absorbance of sample treatment group/absorbance of no treatment group) X 100

2-3. Comparison of antioxidant effects according to raw materials-ABTS radical scavenging ability

Antioxidant effect using ABTS radical was measured by modifying the method of Vanden Berg et al. by using the method that ABTS freeradical generated by reaction with potassium persulfate is removed by antioxidant substances in the sample, and the color of cyan, which is characteristic of the radical, is decolorized ( Vanden Berg R. Haenen GR. Vanden Berg H. Bast A. Applicability of an improved Trolox equivalent anti-oxidant capacity (TEAC) assay for evaluation of anti-oxidant capacity measurements of mixture, Food Chem. 1999;66 p511-517)

7.4mM 2,2-azino-bis(3-rthylbenzthiazoline-6-sulfonic acid) (ABTS) and 2.6Mm potassium persulfate were mixed at a final concentration and left for 24 hours in the dark at room temperature to form ABTS+, and absorbance at 734 nm. It was diluted with distilled water so that the value became 0.75 (±0.1). 100 µl of samples for each concentration were added to 900 µl of the diluted ABTS radical solution and measured 10 minutes after mixing. The ABTS radical scavenging ability was calculated by the following equation 2, and the results are shown in Fig. 8, and antioxidants of black rice at all concentrations It can be seen that the effect is most excellent.

[Equation 2]

ABTS scavenging ability(%)= (1-absorbance in sample treatment area / absorbance in no treatment area) x 100

Example 3. Essence and lotion preparation

As an aspect of the cosmetic composition according to the present invention, an essence composed of the composition shown in Table 2 below and a lotion composed of the composition shown in Table 3 were prepared.

turn Essence composition Content (% by weight) One Purified water Up to 100 2 glycerin 15% 3 Butylene Glycol 5% 4 Squalane 2% 5 Niacinamide 2% 6 Hydrolyzed Rice Protein 2% 7 Octyldodecyl Myristate One% 8 1,2-hexanediol One% 9 Betaine 0.8% 10 lanolin 0.5% 11 Pentaerythrityl tetraethylhexanoate 0.5% 12 Cyclohexasiloxane 0.5% 13 Dimethicone 0.5% 14 Cyclopentasiloxane 0.4% 15 Stearic Acid 0.3% 16 PEG-100 Stearate 0.3% 17 Caprylyl Glycol 0.3% 18 Stearyl alcohol 0.2% 19 Cetyl alcohol 0.2% 20 Glyceryl stearate 0.2% 21 Tromethamine 0.1% 22 Carbomer 0.1% 23 Spices 0.1% 24 Xanthan gum 0.1% 25 Polysorbate 20 0.1% 26 Disodium EDTA 0.03%

turn Lotion composition Content (% by weight) One Purified water Up to 100 2 glycerin 10% 3 Hydrogenated Polydecene 5% 4 Cetearyl alcohol 3% 5 Pentaerythrityl adipate/caprate/caprylate/heptanoate 2.5% 6 Hydrolyzed Rice Protein 2% 7 Propanediol 1.0% 8 1,2-hexanediol 1.0% 9 ethanol 0.5% 10 Glyceryl Stearate SE 0.5% 11 Myristyl alcohol 0.3% 12 Myristyl glucoside 0.2% 13 Butylene Glycol 0.2% 14 Ceteares-20 0.2% 15 Dimethicone 0.2% 16 Tocopheryl acetate 0.1% 17 Acrylate/C10-30 alkyl acrylate crosspolymer 0.1% 18 Spices 0.1% 19 Tromethamine 0.1%

Example 4. Skin moisture improvement test of essence and lotion

(1) Skin moisture improvement test method

Through the Semyung University Oriental Medicine Bio Industry Clinical Support Center, 20 adult women aged 20 to 55 were targeted, and the test site was kept clean and dry in order to equalize the measurement conditions of the subjects. It was carried out after taking stability in a space where the constant temperature and humidity (22±2℃, relative humidity 40~60%) was maintained.

For moisture measurement, the forearm was measured using a Corneometer (CM825, Courage+Khazaka electronic GmbH, Germany), and the average value was calculated by measuring three values. In Corneometer, the higher the moisture, the higher the measurement value, and the measurement coefficient is an Arbitrary unit (A.U.).

The essence of the present invention was applied to the selected test area (1.5cm X 1.5cm) of the forearm on 4 sites in ^{an amount of 2µl/cm 2} using a micro pipette, and the measurement was performed before product application, 30 minutes after application Each was carried out after 3 hours and 6 hours after application.

(2) Skin moisture improvement test result

The moisture measurement results over time after product application are shown in Table 4 below.

division Before application 30 minutes after application 3 hours after application 6 hours after application No application 31.24 30.98 30.74 30.39 essence 31.31 63.31 56.44 52.51 Lotion 31.40 58.31 54.39 52.67

(Unit: A.U.) When the essence according to the present invention was applied, it was measured as 63.31 AU after 30 minutes of application, 56.44 AU after 3 hours of application, and 52.51 AU after 6 hours of application. When the lotion according to the present invention was applied, 58.31 AU after 30 minutes of application , 54.39 AU after 3 hours of application and 52.67 AU after 6 hours of application, both of the essence and lotion according to the present invention showed high values compared to the non-application area.

Example 5. Safety test of essence and lotion on skin patch

(1) Safety test method for skin patch

Through the Semyung University Oriental Medicine Bio Industry Clinical Support Center, 30 adult women aged 20 to 55 years old were evaluated for skin irritation according to the evaluation criteria presented by the International Society for Contact Dermatitis. The test site was kept clean and dry, and the skin was stabilized in a space where constant temperature and humidity (22±2℃, relative humidity 40~60%) was maintained for at least 30 minutes.

Specifically, the test site was wiped with 70% ethanol, dried, and then 25µl of the essence and lotion according to the present invention were dropped into the Finn Chamber, and then fixed on the back of the subject. The patch was removed after 24 hours, and after removing the patch, the test site was marked with a Marking Pen, and the test site was observed after 30 minutes, 24 hours, and 48 hours.

(2) Safety test result for skin patch

The criteria provided by the International Contact Dermatitis Research Group (ICDRG) are shown in Table 5 below.

sign Criteria -
±
+
++
+++ Negative
Doubtful or slight reaction and erythema
Erythema + Induration
Erythema + Induration + Vesicle
Erythema + Induration + Bullae - ± + ++ +++ Score 0 0.5 One 2 3

(※ Skin reaction criteria 1. Negative (-): No irritation

2.Doubtful or slight reaction and erythema (±): no irritation

Faint or barely detectable mild erythema

3. Erythema + Induration (+): light stimulation

Erythema, swelling, and papules with distinct but weak

4.Erythema + Induration + Vesicle (++): Heavy stimulation

Pronounced erythema, papules, and vesicles

5. Erythema + Induration + Bullae (+++): Strong stimulation

Severe erythema and blisters, crust formation)

In the present invention, the average skin reactivity (Mean score) was calculated according to Equation 3 below, and the result determination table based on the Mean score is shown in Table 6 below.

[Equation 3]

i = Subject number 30 minutes after patch removal

j = subject number after 24 hours of patch removal

k = subject number 48 hours after patch removal

Score _i,j,k = Each evaluation result is scored according to ICDRG criteria after 30 minutes, 24 hours, and 48 hours of patch removal.

Skin Patch Test Results Judgment Table Grade Mean Score Non-irritating (1) 0.00-0.75 Unstimulated (2) 0.76-1.50 Light stimulation (3) 1.51-2.50 Heavy stimulation (4) 2.51-4.00 Strong stimulation (5) 4.01 or higher

The results of the skin patch test according to the determination table are shown in Tables 7 and 8 below.

Skin patch test result of the essence according to the present invention No. Subject Initial 30 minutes after patching 24 hours after patch 48 hours after patch One KIS - - - 2 JMH - - - 3 KSH-1 - - - 4 HYM - - - 5 PBK - - - 6 PMH - - - 7 CEY - - - 8 PMJ - - - 9 YJS - - - 10 SYH - - - 11 LHJ - - - 12 PHM - - - 13 KSH-2 - - - 14 PJO - - - 15 PJJ - - - 16 KHJ - - - 17 GEJ - - - 18 KYJ - - - 19 KAR - - - 20 JDH - - - 21 CSO - - - 22 BMH - - - 23 KSJ - - - 24 SYJ - - - 25 KYS - - - 26 SYM - - - 27 KHS - - - 28 YEJ - - - 29 YYH - - - 30 JHJ - - - Responsiveness ± 0 0 0 + 0 0 0 ++ 0 0 0 +++ 0 0 0 Mean Score 0.00 Judgment No irritation

As shown in Table 7 above, according to the results of the skin patch test of the essence according to the present invention, it can be seen that the mean score for all subjects was 0, indicating a non-irritation result.

Results of the patch test of the lotion according to the present invention No. Subject Initial 30 minutes after patching 24 hours after patch 48 hours after patch One KIS - - - 2 JMH - - - 3 KSH-1 - - - 4 HYM - - - 5 PBK - - - 6 PMH - - - 7 CEY - - - 8 PMJ - - - 9 YJS - - - 10 SYH - - - 11 LHJ - - - 12 PHM - - - 13 KSH-2 - - - 14 PJO - - - 15 PJJ - - - 16 KHJ - - - 17 GEJ - - - 18 KYJ - - - 19 KAR - - - 20 JDH - - - 21 CSO - - - 22 BMH - - - 23 KSJ - - - 24 SYJ - - - 25 KYS - - - 26 SYM - - - 27 KHS - - - 28 YEJ - - - 29 YYH - - - 30 JHJ - - - Responsiveness ± 0 0 0 + 0 0 0 ++ 0 0 0 +++ 0 0 0 Mean Score 0.00 Judgment No irritation

As shown in Table 8, it can be seen that the non-irritating result was shown as a mean score of 0 for all subjects in the lotion according to the present invention.

Claims (9)

Cosmetic composition for improving skin moisture containing hydrolyzed rice protein.
The method of claim 1, wherein the cosmetic composition comprises 10 to 20% by weight of glycerin, 4 to 6% by weight of butylene glycol, and squalane 1 based on 100% by weight of the total cosmetic composition. To 3% by weight, 1 to 3% by weight of niacinamide, 1 to 3% by weight of Hydrolyzed Rice Protein, 0.5 to 1.5% by weight of Octyldodecyl Myristate, 1,2 -Hexanediol (1,2-Hexanediol) 0.5 to 1.5% by weight, betaine (betaine) 0.6 to 1% by weight, lanoline 0.4 to 0.6% by weight, pentaerythrityl tetraethylhexanoate (Pentaerythrityl Tetrathylhexanoate) 0.4 to 0.6 wt%, Cyclohexasiloxane 0.4 to 0.6 wt%, Dimethicone 0.4 to 0.6 wt%, Cyclopentasiloxane 0.4 wt%, Stearic Acid 0.2 To 0.4 wt%, PEG-100 Stearate 0.2 to 0.4 wt%, Caprylyl Glycol 0.2 to 0.4 wt%, Stearyl alcohol 0.1 to 0.3 wt% , Cetyl Alcohol (Cetyl Alcohol) 0.1 to 0.3% by weight, Glyceryl Stearate (Glyceryl Stearate) 0.1 to 0.3% by weight, Tromethamine (Tromethamine) 0.05 to 0.15% by weight, Carbomer (Carbomer) 0.05 to 0.15% by weight, Perfume 0.05 to 0.15 wt%, Xanthan Gum 0.05 to 0.15 wt%, Polysorbate 20 0.05 to 0.15 wt%, Disodium EDTA (Disodu im EDTA) A cosmetic composition for improving skin moisture, characterized in that consisting of 0.02 to 0.04% by weight and purified water.
The cosmetic composition for improving skin moisture according to claim 2, wherein the cosmetic composition is an essence formulation.
The method of claim 1, wherein the cosmetic composition comprises 8 to 12% by weight of glycerin, 4 to 6% by weight of hydrogenated polydecene, and cetearyl alcohol based on 100% by weight of the total cosmetic composition. (Cetearyl Alcohol) 2 to 4 wt%, Pentaerythrityladipate/Caprate/Caprylate/Heptanoate 2 to 4 wt%, Hydrolyzed Rice Protein ) 1 to 3% by weight, propanediol 0.5 to 1.5% by weight, 1,2-hexanediol (1,2-Hexanediol) 0.5 to 1.5% by weight, ethanol (Ethanol) 0.4 to 0.6% by weight, glycerol Glyceryl Stearate SE 0.4 to 0.6 wt%, Myristyl Alcohol 0.2 to 0.4 wt%, Myristyl Glucoside 0.1 to 0.3 wt%, Butylene Glycol 0.1 To 0.3% by weight, 0.1 to 0.3% by weight of Ceteaeth-20, 0.1 to 0.3% by weight of dimethicone, 0.05 to 0.15% by weight of tocopheryl acetate, acrylate/C10- 30 Alkyl Acrylate Crosspolymer (Acrylates/C10-30 Alkyl Acrylate Crosspolymer) 0.05 to 0.15% by weight, fragrance 0.05 to 0.15% by weight, tromethamine 0.05 to 0.15% by weight, and purified water Cosmetic composition for improving moisture.
The cosmetic composition for improving skin moisture according to claim 4, wherein the cosmetic composition is a lotion formulation.
S1) adding purified water after pulverizing the rice and heating it to 45°C;
S2) reacting a reaction solution in which 1% by weight of glycolytic enzyme is mixed with respect to 100% by weight of the total rice for 5 hours;
S3) treating the reaction solution at 85 to 95° C. for 30 minutes to deactivate the glycolytic enzyme;
S4) cooling the reaction solution to 45°C and mixing it with 0.1N aqueous sodium hydroxide solution to adjust the pH to 7;
S5) adding a protease to the reaction solution to proceed with the enzyme reaction at 45° C. for 5 hours;
S6) treating the reaction solution at 85 to 95°C for 30 minutes to inactivate the protease;
S7) cooling the reaction solution to 30 ℃ and extracting the rice protein using 30% ethanol for 48 hours;
S8) filtering the extracted rice protein (0.45um~3um) and concentrating at 65~75℃;
S9) adding a solvent (1,3-butylene glycol 30%) to the concentrated rice protein and then filtering (0.2um~0.45um);
Method for producing hydrolyzed rice protein produced through.
[7] The method of claim 6, wherein the rice is at least one selected from large-eyed brown rice, nokchal brown rice, scented brown rice, black rice, or hongchal brown rice.
The method of claim 6, wherein the glycolytic enzyme is a yeast purification enzyme.
The method of claim 6, wherein the protease is at least one of Alcalase 2.4L, Bromelain 1200 GDU, or Papain.

Images