KR20210049642A - Pharmaceutical composition comprising the extraction of cattail pollen as an effective component for prevention or treatment of diabetes and health functional food comprising the same - Google Patents

Abstract

The present invention relates to a pharmaceutical composition and a health functional food for preventing or treating diabetes comprising an extract of pollen of Typha augustifolia L. as an active ingredient, and more specifically, to a pharmaceutical composition and a health functional food for preventing or treating/alleviating diabetes, comprising an ethyl acetate fraction of an ethanol extract of pollen of Typha augustifolia L. as an active ingredient. As an active ingredient of the anti-diabetic composition of the present invention, the extract of pollen of Typha augustifolia L. exhibits an excellent effect, which can be used in the medicine and health functional food for preventing and alleviating diabetes as proven through an embodiment of the present specification. Furthermore, the extract of pollen of Typha augustifolia L. of the present invention shows no loss of starch degrading enzyme inhibitory activity even under acidic conditions of pH 2 and in plasma, and is easily processed into various forms such as liquids, creams, powders, pills, tablets, etc., and thus is very useful in the pharmaceutical industry and food industry.

Description

Pharmaceutical composition and health functional food for prevention or treatment of diabetes containing the extract of pumice pollen as an active ingredient {PHARMACEUTICAL COMPOSITION COMPRISING THE EXTRACTION OF CATTAIL POLLEN AS AN EFFECTIVE COMPONENT FOR PREVENTION OR TREATMENT OF DIABETES AND HEALTH FUNCTIONAL FOOD COMPRISING THE SAME}

The present invention relates to a pharmaceutical composition and health functional food for the prevention or treatment of diabetes containing a pollen (Pollen of Typha augustifolia L.) extract as an active ingredient, and more specifically, an ethanol extract of a pollen of Typha It relates to a pharmaceutical composition and health functional food for preventing or treating/improving diabetes using as an active ingredient.

Diabetes, one of the most common diseases in modern people, is a type of metabolic disease such as insufficient secretion of insulin or inability to function normally. It is characterized by high blood glucose concentration and causes various symptoms and signs due to high blood sugar.

Diabetes is classified into type 1 and type 2. Type 1 diabetes is caused by the inability to produce insulin at all due to genetic effects, and type 2 diabetes is caused by poor insulin function and insulin resistance. ) Is known as the cause. In particular, type 2 diabetes is known to be largely affected by environmental factors such as high calorie, high fat, high protein diet, lack of exercise, and stress due to westernization of diet.

For the treatment of diabetes, insulin injection is essential in the case of type 1 diabetes, and lifestyle correction and drug treatment are necessary in the case of type 2 diabetes. Nide) and a delaying agent for carbohydrate absorption in the small intestine (glucobi: ingredient name-acarbose, bason: ingredient name-voglibose), and the like have been used.

On the other hand, Typha augustifolia L. is a perennial monocotyledonous plant of the cattail family. It grows in wetlands such as ponds, rivers, rivers, and swamps, and can be found in. , Leaves are linear, narrow and long, green. In Korea, it is mainly used for ornamental and flower arrangements, and caterpillar pollen (pollen) is used in the private sector for hemostasis, pain relief, and diuresis. In oriental medicine, especially the pollen is called phohwang. It has a sweet taste and is not toxic, so it has a wide range of uses such as hemostatic agents, uterine bleeding, hemostatic, hemorrhagic hari, prolapse, anti-inflammatory diuretics, hemorrhoids, hypoplasia, menstrual impurities, cystitis, etc. It has been used as a medicinal material.

Pollen is a reproductive organ of a vascular plant that makes seeds. It is a structure surrounded by a sturdy shell to protect male gametes, and is transported mainly by wind or insects to reach female gametes and fertilize them to form seeds. Pollen is divided into chungmae pots mediated by insects and air pollen pots mediated by wind, and chungmae pots are divided into single plant pots such as darae pots and acorn pots, and miscellaneous pots in which various plant pots are collected. There is a potted plant.

Usually, pollen contains 20 to 25% crude protein, 7 to 15% crude lipid, 2 to 5% crude ash, 20 to 50% crude carbohydrate, and 10 to 15% moisture, so it is excellent nutritionally and contains various physiologically active substances. Including, it has been used as a nutritional supplement and traditional medicine. However, the pollen is composed of a thick layer called'sporopollenin', an extine and an intime, so it cannot be digested or decomposed by itself, so the bioavailability is low, 10-15% (Pyeon HI, 2018, Journal of Life Science 28: 605~614), microbiological contamination may occur due to various plants, and there is also a disadvantage of easily decaying. In addition, toxic substances (1,2-dehydro pyrollizidine alkaloids) suspected of hepatotoxicity, lung toxicity, and carcinogenicity have been identified in pollen of Western borer flowers (Boppre, M et al., J. Agric. Food Chem. 53, 594- 600).

Therefore, the research on the existing pollen is mainly focused on the development of extraction conditions and pretreatment methods for enhancing digestion and absorption by decomposing the nutrient properties of the pollen and the robust outer shell. In recent years, research on useful physiological activity is being conducted, and it is known to be effective in various diseases such as vascular and circulatory system, digestive system, metabolic system, and aging. Antibacterial and antioxidant effects, immunity enhancing effects, prostatic hyperplasia and prostatitis treatment Effects, etc. have been reported (Choi et al., 2007, Korean J. Food preserv. 14, 87-93; Abouda et al., 2011, J. Microbiol. 6, 376-384; Li et al., 2009, Carbohydrate Polymers 78 , 80-88; In-pyo Hong et al., 2016, Journal of Apiculture 31, 219-225; In-pyo Hong, 2014, Journal of Apiculture 29, 137-142; Yong-ki Park et al., 2015. Journal of Apiculture 30, 299-306; Seok-joong Kim et al., 2005 , Korean Journal of Food Science and Technology 37, 833-837).

Until now, the anti-diabetic effect of pollen has been partially recognized by the private sector, but some have contradicted opinions as it is understood as a food ingredient that diabetic patients should avoid due to the high sugar content of pollen. Among the by-products of beekeeping, royal jelly and propolis have an antidiabetic effect, but pollen is very difficult to understand the diabetes-related activity because the composition of pollen varies greatly depending on the type of plant.

The antidiabetic effect of pollen on scientific basis was determined by the high molecular weight polysaccharides of caterpillar pollen (Lei X et al., 2018. J Ethnopharmacol. 224:169-176) and macromolecular polysaccharides of the pollen (Li X et al. 2017, Molecules. 22(5)). : The antidiabetic effect of E699) is known, but this is limited to the effect of improving insulin resistance and diabetic complications caused by high molecular polysaccharides in pollen. In addition, it has been reported that the flavone component of caterpillar pollen improves insulin resistance (Feng, Xiao-Tao et al., 2014, Bioscience, Biotechnology, Biochemistry 78: 1738-1742), and date palm pollen relieves glycemia. It is known (Mohamed, Nema A. 2018, Biomedicine & Pharmacotherapy 97: 9-18).

However, until now, it has not been known that a specific fat-soluble component of caterpillar pollen exhibits antidiabetic activity through strong α-glucosidase and β-amylase inhibition.

Lei X et al., 2018. J Ethnopharmacol. 224:169-176 Li X et al. 2017, Molecules. 22(5): E699 Feng, Xiao-Tao et al., 2014, Bioscience, Biotechnology, Biochemistry 78: 1738-1742 Mohamed, Nema A. 2018, Biomedicine & Pharmacotherapy 97: 9-18

The present invention was conceived to solve the problems of the prior art as described above, and the problem to be solved in the present invention is to provide a pharmaceutical composition for preventing or treating diabetes containing a pollen extract as an active ingredient and a health functional food I want to.

In order to solve the above problems, the present invention provides a pharmaceutical composition for preventing or treating diabetes containing an ethanol extract of Pollen of Typha augustifolia L. as an active ingredient.

It is preferable that the potted plant ethanol extract is a hexene fraction or an ethyl acetate fraction of the potted plant ethanol extract.

In addition, the present invention provides a health functional food for preventing or improving diabetes containing an ethanol extract of Pollen of Typha augustifolia L. as an active ingredient.

It is preferable that the potted plant ethanol extract is a hexene fraction or an ethyl acetate fraction of the potted plant ethanol extract.

Budeul pollen extract as an active ingredient of the antidiabetic composition of the present invention, as proven through the examples of the present specification, has excellent effects that can be used as medicines and health functional foods for preventing or improving diabetes. In addition, the Budeul pollen extract of the present invention has excellent thermal stability and does not show any loss of starch degrading enzyme inhibitory activity even in the acidic condition of pH 2 and plasma, so it can be easily used in various forms such as liquid, cream, powder, pills, tablets, etc. Because it can be processed, it can be very useful in the pharmaceutical industry and the food industry.

1 is a photograph of a potted plant.
2 is a microscope (200 times) observation photograph of a potted plant.

Hereinafter, the present invention will be described in detail.

In order to test the anti-diabetic efficacy of the caterpillar pollen, the inventors of the present invention prepared caterpillar pollen extract by a certain method, and recovered the ethanol extract thereof as an antidiabetic active ingredient, and the ethanol extract has thermal stability and acid stability. By confirming that it has excellent characteristics, it was attempted to utilize the potted plant extract as an antidiabetic composition.

Specifically, the present inventors prepared hot water and ethanol extracts for the potted pots in order to develop an antidiabetic composition using potted plants known to be effective in various diseases such as vascular and circulatory system, digestive system, and metabolic system, After preparing sequential organic solvent fractions from the ethanol extract, their inhibitory activity against β-amylase and α-glucosidase was evaluated. As a result, potent β-amylase and α- in the ethanol extract of Budeul pollen and its hexene fraction and ethyl acetate fraction. The inhibitory activity of the glucosidase enzyme was confirmed.

Accordingly, the present invention provides a pharmaceutical composition for preventing or treating diabetes containing an ethanol extract of Pollen of Typha augustifolia L. as an active ingredient.

It is preferable that the potted plant ethanol extract is a hexene fraction or an ethyl acetate fraction of the potted plant ethanol extract.

In addition, the present invention provides a health functional food for preventing or improving diabetes containing an ethanol extract of Pollen of Typha augustifolia L. as an active ingredient.

It is preferable that the potted plant ethanol extract is a hexene fraction or an ethyl acetate fraction of the potted plant ethanol extract.

Hereinafter, a method for preparing the ethanol extract of Budeul pollen of the present invention and an antidiabetic active fraction thereof and an efficacy experiment will be described in more detail.

The inventors of the present invention, in order to achieve the object of the present invention, preparing an extract from a potted plant; A step of preparing sequential organic solvent fractions of hexene, ethyl acetate, and butanol from the ethanol extract of puppet pollen and preparing a water residue obtained thereafter; Antidiabetic activity evaluation step of the extracts and fractions; And a stability investigation step of the ethanol extract, the hexene fraction and the ethyl acetate fraction.

The "Budeul pollen extract" included in the composition of the present invention may be obtained by extracting the Budeul pollen with an organic solvent and filtering the extract using a filter net of 0.06mm or less, and concentrating it under reduced pressure.

The solvent used in the present invention includes water (cold water, hot water), alcohol, anhydrous or hydrated lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, alcohol, propanol, butanol, etc.), and a mixed solvent of the lower alcohol and water. Hot water, or 95% ethanol extraction is most preferred.

As a preferred embodiment, the potted plant of the present invention may be extracted with hot water or ethanol. In addition, the hot water or ethanol extract may be sequentially or separately fractionated with an organic solvent of hexene, ethyl acetate and butanol to additionally obtain a hexene fraction, an ethyl acetate fraction, a butanol fraction, and a water residue.

The ethanol extract of Budeul pollen of the present invention and the hexene or ethyl acetate fraction thereof may be prepared as a powder through a conventional powdering process such as vacuum drying, freeze drying, or spray drying. The active extracts and fractions are not decomposed by various degrading enzymes in plasma, and maintain activity even at a heat treatment of 100° C. and a pH in the stomach of the human body at pH 2.

The ethanol extract of puppet pollen of the present invention, its hexene fraction and ethyl acetate fraction exhibit potent inhibitory activity against starch degrading enzymes against β-amylase and α-glucosidase, and thus type 1 diabetes, type 2 diabetes or diabetic retinopathy, diabetic It can be used as a material for a pharmaceutical composition and health functional food related to the prevention and treatment/improvement of diabetic complications such as nephropathy.

As a preferred embodiment, the antidiabetic composition of the present invention can be applied as a pharmaceutical composition.

The pharmaceutical composition is formulated in various forms such as oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, and injections of sterile injectable solutions according to a conventional method according to each purpose of use. It may be used, and may be administered orally, or administered through various routes including intravenous, intraperitoneal, subcutaneous, rectal, topical administration, and the like. Such pharmaceutical compositions may further include a carrier, excipient, or diluent, and examples of suitable carriers, excipients or diluents that may be included include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, Starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, amorphous cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil And the like. In addition, the pharmaceutical composition of the present invention may further include a filler, an anti-aggregating agent, a lubricant, a wetting agent, a flavoring agent, an emulsifying agent, a preservative, and the like.

The pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount. In the present invention, "pharmaceutically effective amount" means an amount sufficient to treat a disease at a reasonable benefit/risk ratio applicable to medical treatment, and the effective dose level is the type of disease, severity, drug activity, Sensitivity to the drug, time of administration, route of administration and rate of excretion, duration of treatment, factors including drugs used concurrently, and other factors well known in the medical field. The pharmaceutical composition of the present invention may be administered as an individual therapeutic agent or administered in combination with another therapeutic agent, may be administered sequentially or simultaneously with a conventional therapeutic agent, and may be administered single or multiple. It is important to administer an amount capable of obtaining the maximum effect in a minimum amount without side effects in consideration of all the above factors, and this can be easily determined by a person skilled in the art.

The effective amount of the component having antidiabetic activity in the pharmaceutical composition of the present invention may vary depending on the age, sex, and body weight of the patient, and is generally 1 to 5,000 mg per body weight, preferably 100 to 3,000 mg daily or every other day. It can be administered or divided into 1 to 3 times a day. However, since it may increase or decrease according to the route of administration, the severity of the disease, sex, weight, age, etc., the dosage amount does not limit the scope of the present invention in any way. The pharmaceutical composition of the present invention can be administered to a subject through various routes. All modes of administration can be expected and can be administered, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dura mater or intra-cerebroventricular injection. In the present invention, "administration" means providing a predetermined substance to a patient by any suitable method, and the route of administration of the pharmaceutical composition of the present invention is oral or parenteral through all general routes as long as it can reach the target tissue. It can be administered orally. In addition, the composition of the present invention may be administered using any device capable of delivering an active ingredient to target cells. In the present invention, the "subject" is not particularly limited, but includes, for example, a human, a monkey, a cow, a horse, a sheep, a pig, a chicken, a turkey, a quail, a cat, a dog, a mouse, a mouse, a rabbit, or a guinea pig. And, preferably, it means a mammal, more preferably a human.

As a preferred embodiment, the antidiabetic composition of the present invention can be applied as a health functional food.

Foods containing an active ingredient having excellent antidiabetic activity of the present invention include, for example, various foods, beverages, gum, tea, vitamin complexes, health supplement foods, etc., and powders, granules, tablets, capsules or beverages. Can be used in the form. In general, the active ingredient of the present invention may be added in an amount of 0.01 to 15% by weight of the total food weight, and the health beverage composition may be added in an amount of 0.02 to 10g, preferably 0.3 to 1g, based on 100 ml. The health functional food of the present invention may contain the compound as an essential component in the indicated ratio as an additional component, as well as food supplementary additives acceptable for food, such as natural carbohydrates and various flavoring agents. Examples of the natural carbohydrates include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and conventional sugars such as polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. Taumatin, rebaudioside A, glysirhizin, saccharin, aspartame, and the like may be used as the flavoring agent. The proportion of the flavoring agent is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the health functional food of the present invention. In addition to the above, the health functional food of the present invention includes a variety of nutrients, vitamins, minerals, synthetic flavors and natural flavors, and other flavoring agents, coloring agents and thickeners, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloids. It may contain thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the health functional food of the present invention may contain pulp for the production of natural fruit juices, fruit juice beverages, and vegetable beverages. These components may be used independently or in combination. The proportion of these additives is generally selected in the range of 0.01 to about 20 parts by weight per 100 parts by weight of the active fraction of the present invention.

Hereinafter, the present invention will be described in more detail through examples. The following examples are only a preferred specific example of the present invention, and the scope of the present invention is not limited to the scope of the following examples.

[Example]

Example 1: Analysis of Physicochemical Properties and Nutritional Components of Cattail Pollen

Caterpillar pollen used in the experiment was a caterpillar pollen harvested in Andong, Gyeongbuk in 2018. It had a lightness of 59.44, a redness of 0.35, and a yellowness of 30.49 as fine granules of jin yellow. Indicated. The moisture content was in a dried state of 12.5%, and when left at room temperature for a long time, moisture was absorbed again (FIG. 1, Table 1). As a result of observation under a microscope (200 times), the caterpillar pollen was a single-grained spherical shape, and the surface was granular, similar to the acorn pollen, and was differentiated from the typical wind plum pollen, Songhwabun, because there was no net patterned wing structure (Fig. 2).

[Table 1] Analysis of Physicochemical Characteristics and Color Difference of Cattail Pollen

As a result of nutritional analysis, crude protein 18.2%, crude fat 1.0%, and crude carbohydrate 64.1% were excellent at 338 kcal/100g, and ash content also showed 4.2%, which contained a large amount of various minerals (Table 2).

[Table 2] Analysis of nutrient composition of caterpillar pollen

Example 2: Preparation of Cattle Pollen Extract and Analysis of Their Useful Components

The potted plants of Example 1 were used to prepare hot water and ethanol extracts. Specifically, 10 times the amount of ethanol was added to the Budeul pollen sample, extracted twice at room temperature, collected and filtered, and concentrated under reduced pressure to prepare an ethanol extract. 10 times of sterilized distilled water was added to the pollen sample, and extraction was repeated at 100° C. for 1 hour twice, followed by filtration and concentration in the same manner as above.

Contents of total polyphenols, total flavonoids, total sugars and reducing sugars were measured by analysis of the components of the pollen extract. For the total polyphenol content, 50 μl of Folin-ciocalteau and 100 μl of Na ₂ CO ₃ saturated solution were added to 400 μl of the extraction sample, and the absorbance was measured at 725 nm after standing at room temperature for 1 hour. Tannic acid was used as a standard reagent. For the total flavonoid content, each sample was extracted with methanol for 18 hours, and 4 ml of 90% diethylene glycol was added to 400 μl of the filtered extraction sample solution, 40 μl of 1 N NaOH was added, and the absorbance was measured at 420 nm after 1 hour reaction at 37°C. As a standard reagent, rutin was used. Reducing sugar was quantified by DNS method and total sugar was quantified by phenol-sulfuric acid method. Their fractionation efficiency and component analysis results are shown in Table 3.

[Table 3] Analysis of Extraction Efficiency and Useful Components of Budeul Pollen Extract

As shown in Table 3, the efficiency of extracting ethanol from the potted plant was 12.5%, which was lower than that of the hot water extraction efficiency (29.8%). In addition, the total polyphenol content of the ethanol extract was 57.4% lower than that of the hot water extract, and the total flavonoid content was similar in the hot water extract and the ethanol extract. In addition, the contents of total sugar and reducing sugar were 2.7 times and 2.65 times higher in the hot water extract than in the ethanol extract, and it was found that most of the hot water extract was composed of sugar.

Example 3: Evaluation of Antidiabetic Activity of Cattle Pollen Extract

The antidiabetic activity of the extract of Caterpillar pollen prepared in Example 2 was evaluated, and in-vitro β-amylase inhibitory activity and α-glucosidase inhibitory activity were evaluated and shown. First, β-amylase (4-alpha-D-glucan maltohydrolase) inhibitory activity was obtained by mixing 2.5 μl of a sample and 25 μl of α-amylase (0.25 U/ml) diluted with 50 mM phosphate buffer (pH 6.8) at 37°C for 10 minutes. After the first reaction, 25 μl of 0.5% soluble starch (Samchun Chemicals Co., Korea) was added and the second reaction was performed at 37°C for 10 minutes, and then the reaction was stopped by heating at 100°C for 5 minutes, and 150 μl of DNS in the reaction solution (3,5-dinitrosalicylic acid, Sigma Co., st. Louis, USA) solution was added, heated at 100° C. for 5 minutes to develop color, and then allowed to cool at room temperature. The color developing solution was calculated by measuring the absorbance at 540 nm.

Inhibition rate (%) = [1-(Enzyme activity after addition of sample/Enzyme activity after addition of control)] x 100

α-glucosidase inhibitory activity was evaluated using pNPG (p-nitrophenol glucoside; Sigma Co., USA), and α-glucosidase (0.25 U/ml) 25 μl diluted with 2.5 μl of sample and 50 mM sodium acetate buffer (pH 5.6). The mixture was first reacted at 37° C. for 10 minutes, and 25 μl of a 1 mM pNPG solution was added, followed by a second reaction at 60° C. for 10 minutes. Thereafter, 25 μl of 1M NaOH was added to stop the reaction, and the inhibition rate was calculated by measuring the absorbance at 405 nm.

Inhibition rate (%) = [1-(Enzyme activity after addition of sample/Enzyme activity after addition of control)] x 100

[Table 4] Antidiabetic activity of extracts of pollen extract

As a result, as shown in Table 4, acarbose, which has been used clinically as a therapeutic agent for diabetes, showed strong β-amylase inhibitory activity and α-glucosidase inhibitory activity in a concentration-dependent manner. In the case of the hot-water extract of Caterpillar pollen, the inhibitory activity of β-amylase and α-glucosidase was not observed at all, but the ethanol extract showed very strong β-amylase and α-glucosidase inhibitory activity at the concentration of 0.5mg/ml.

Example 4: Preparation of sequential organic solvent fractions of ethanol extract of Budeul pollen and analysis of useful components thereof

Budeul pollen ethanol extract of Example 2 The ethanol extract was subjected to sequential organic solvent fractionation with hexene, ethyl acetate, and butanol, and finally the water residue was recovered. According to the analysis method described in Example 2, their fractionation efficiency and component analysis results are shown in Table 5.

[Table 5] Analysis of fractionation efficiency and useful components of sequential organic solvent fractions of ethanol extract of Budeul pollen

As shown in Table 5, the most part of the ethanol extract of Caterpillar pollen was a hexene fraction (70.3%), and it was found that the ethanol extract of Caterpillar pollen contained a significant amount of fat-soluble components. They accounted for 12.8% and 12.1%. The ethyl acetate fraction showing the lowest yield represented 9.5% of the ethanol extract of Caterpillar pollen, and 1.18g per 100g of Caterpillar pollen could be recovered. In addition, the analysis of the total polyphenol and total flavonoid content showed the highest polyphenol content (89.0 mg/g) and flavonoid content (101.6 mg/g) in the ethyl acetate fraction. This was 9.1 times higher polyphenol content and 5.95 times higher flavonoid content compared to the water residue showing the lowest content. Therefore, the ethyl acetate fraction of the ethanol extract of Caterpillar pollen was expected to exhibit various physiological activities, and as a result of actual analysis, the fraction showed strong antioxidant activity and nitrite scavenging activity. Therefore, the ethyl acetate fraction of caterpillar pollen is expected to additionally contribute to improving blood circulation and antithrombotic activity by exhibiting excellent antioxidant and carcinogenic activity.

Example 5: Evaluation of Antidiabetic Activity of Sequential Organic Solvent Fractions of Ethanol Extract of Budeul Pollen

The antidiabetic activity of the sequential organic solvent fractions of the ethanol extract of Caterpillar pollen prepared in Example 4 was evaluated according to the method described in Example 3.

[Table 6] Antidiabetic Activity of Sequential Organic Solvent Fractions of Ethanol Extract of Budeul Pollen

As a result, as shown in Table 6, among the fractions of the ethanol extract, the hexene fraction and the ethyl acetate fraction (0.5 mg/ml) showed 17.4 to 18.5% of β-amylase inhibition and 8.1 to 14.8% of α-glucosidase inhibition. I got it. The antidiabetic activity of the hexene fraction and ethylacetate fraction of the ethanol extract of Caterpillar pollen showed stronger antidiabetic activity when treated with high concentration, and showed a concentration-dependent inhibitory activity. Therefore, it was confirmed that the hexene fraction and ethyl acetate fraction of the ethanol extract of Caterpillar pollen can be used for the prevention and treatment of type 2 diabetes.

Example 6: Plasma, acid, and thermal stability evaluation of ethanol extract, hexene or ethyl acetate fraction thereof

Plasma stability, thermal stability, and acid stability for antidiabetic activity were confirmed with respect to the antidiabetic activity of the ethyl acetate fraction of the Ethanol extract, its hexene fraction and the ethyl acetate fraction obtained in Examples 2 and 4 above. Caterpillar pollen extract, its hexene and ethyl acetate fractions were heat treated at 100°C for 1 hour, treated at pH 2 (0.01M HCl) for 1 hour, decreased β-amylase and α-glucosidase inhibitory activity even when treated in plasma for 1 hour. Rarely appeared. Therefore, it was confirmed that the pollen extract, the hexene fraction and the ethyl acetate fraction contained an antidiabetic active substance having acid resistance and heat resistance.

Claims (3)

Budeul pollen (Pollen of Typha augustifolia L.) A pharmaceutical composition for preventing or treating diabetes containing ethanol extract as an active ingredient. The pharmaceutical composition according to claim 1, wherein the potted plant ethanol extract is a hexene fraction or an ethyl acetate fraction of the potted plant ethanol extract. A health functional food for preventing or improving diabetes, comprising as an active ingredient according to claim 1 or 2.

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