KR20210041680A - Antioxidant and moisturizing cosmetic composition containing plant extracts - Google Patents

Abstract

The present invention relates to an antioxidant and moisturizing cosmetic composition containing a plant extract. According to the present invention, it is possible to provide an antioxidant and moisturizing cosmetic composition containing a plant extract which contains a Ligularia stenocephala extract, an Orostachys japonica extract, a Cymbopogon citratus extract, and a Rosa multiflora extract.

Description

Antioxidant and moisturizing cosmetic composition containing plant extracts

The present invention relates to an antioxidant and moisturizing cosmetic composition containing a plant extract, and in more detail, by containing a plant extract including an extract extracted from the above-ground part of gondalbi, rock brush, lemongrass, and brier, it has excellent antioxidant and moisturizing functions. In addition, it relates to an antioxidant and moisturizing cosmetic composition containing a plant extract that exhibits an antiseptic effect as well.

As human skin ages, various internal and external factors change it.

In other words, internally, the secretion of various hormones that regulate metabolism decreases, the function of immune cells and the activity of cells decrease, thereby reducing the biosynthesis of immune proteins and biological components necessary for the living body, and externally, due to the destruction of the ozone layer. As a result, the amount of ultraviolet rays reaching the surface of the sun's rays increases, and as environmental pollution becomes more intense, free radicals and active harmful oxygen increase, so that the thickness of the skin decreases, wrinkles increase, and elasticity decreases. In addition, the complexion of the skin becomes dull, and skin problems occur frequently, and various changes occur, such as increased spots, freckles and age spots.

As aging progresses, the content and arrangement of collagen, elastin, hyaluronic acid, and glycoproteins, which are substances constituting the skin, change or decrease. Symptoms arise, and oxidative stress due to free radicals and free radicals and free radicals occurs. In addition, cyclooxygenase-2 (Cox-2, cyclooxygenase), an enzyme that produces proinflammatory cytokines, is known to cause inflammation in most cells that make up the skin due to aging or ultraviolet rays. Biosynthesis increases, and the biosynthesis of matrix metalloproteinase (MMP), an enzyme that degrades skin tissue by these inflammatory factors, increases, and NO (nitric oxide) production by iNOS (inducible nitric oxide synthase) is increased. It is known to increase.

In other words, external factors such as a decrease in cellular activity due to naturally occurring endogenous aging and a decrease in the biosynthesis of substrate materials due to micro-inflammation, an increase in stress caused by various harmful environments, and an increase in reactive oxygen species caused by sunlight. Decomposition and degeneration are accelerated by the skin, resulting in destruction and thinning of the skin, leading to various symptoms of skin aging.

Therefore, it is a reality that many studies are being conducted on active ingredients that can prevent and improve such aging phenomena. As an example, Korean Patent Publication No. 10-2002-0080657 has been disclosed.

On the other hand, the skin is a representative organ that exhibits an immune response while retaining moisture in the body and performing an essential barrier function to protect the inside from the invasion of external factors.

Here, hyaluronic acid (HA), a representative factor that maintains skin moisture, is one of the three major elements of the skin along with collagen and elastin, and has a strong hydrophilic property to form a gel by combining with water, which plays an important role in maintaining skin moisture. It is known as a natural moisturizer.

Therefore, substances that induce synthesis of hyaluronic acid or inhibit decomposition are effective in moisturizing the skin and preventing aging.In particular, among hyaluronic acid synthase (HAS), HAS2 and HAS3 are known to play a crucial role in the synthesis of hyaluronic acid. , Recently, studies to promote the production of hyaluronic acid through the increase of HAS gene expression have been actively conducted.

Therefore, in the present invention, it was confirmed that extracts such as gondalbi, rock brush, lemongrass, brier, etc. among resource plants in the Namwon-Jirisan area, have antioxidant and moisturizing functions, and accordingly, using gondalbi, rock brush, lemongrass, brier and other extracts. To develop a cosmetic composition with excellent antioxidant and moisturizing functions.

In order to solve the above problems, the present invention contains a plant extract including an extract extracted from the above-ground part of gondalbi, rock brush, lemongrass, and brier, thereby having an excellent antioxidant and moisturizing function, as well as a plant extract showing an antiseptic effect. It is an object to provide an antioxidant and moisturizing cosmetic composition.

In order to solve the above problems, the antioxidant and moisturizing cosmetic composition comprising a plant extract according to an embodiment of the present invention is an antioxidant containing a plant extract including gondalbi extract, rock brush extract, lemongrass extract and brier extract, and It is possible to provide a moisturizing cosmetic composition.

Here, the gondalbi extract is characterized by having quercitrin, chlorogenic acid, and rutin as indicator substances.

In addition, the rock brush extract is characterized by having gallic acid and epigallocatechin gallate (EGCG) as indicator substances.

In addition, the lemongrass extract is characterized by having chlorogenic acid and p-coumaric acid as indicator substances.

In addition, the brier extract is characterized in that it has astragalin (Astragalin) and gallic acid (Gallic acid) as indicator substances.

In addition, the plant extract is characterized in that each extract is extracted using water or an organic solvent as a solvent.

In addition, the plant extract is characterized in that it comprises the gondalbi extract, rock brush extract, lemongrass extract, and brier extract in a weight ratio of 1 to 1.5: 1.5 to 2: 1 to 1.5: 0.5 to 1.

In addition, the plant extract is characterized in that it is contained in an amount of 3 to 8% by weight, based on the total weight of the cosmetic composition.

In addition, the plant extract may further include a barley bangle extract.

In addition, the plant extract may further include sapodilla extract and peppermint fermentation broth.

In addition, emulsion, cream, lotion, essence, pack, suspension, paste, gel, powder, lipstick, makeup base, foundation, lotion, ointment, gel, patch, essence, cleansing foam, cleansing cream, cleansing water, soap, shampoo It is possible to provide a cosmetic composition for antioxidant and moisturizing, characterized in that the formulation of, rinse, spray.

The antioxidant and moisturizing cosmetic composition containing the plant extract according to the embodiment of the present invention as described above contains plant extracts including extracts extracted from the above-ground parts of gondalbi, rock brush, lemongrass, and brier, thereby providing more antioxidant and moisturizing functions. Can be expressed effectively.

In addition, it has an excellent antioxidant and moisturizing function, as well as an antiseptic effect.

Hereinafter, the description of the present invention with reference to the drawings is not limited to a specific embodiment, and various transformations may be applied and various embodiments may be provided. In addition, the content described below should be understood to include all conversions, equivalents, and substitutes included in the spirit and scope of the present invention.

In the following description, terms such as first and second are terms used to describe various elements, and their meanings are not limited, and are used only for the purpose of distinguishing one element from other elements.

The same reference numbers used throughout this specification denote the same elements.

The singular expression used in the present invention includes a plurality of expressions unless the context clearly indicates otherwise. In addition, terms such as "comprise", "include" or "have" described below are intended to designate the presence of features, numbers, steps, actions, components, parts, or combinations thereof described in the specification. It is to be construed and not to preclude the possibility of the presence or addition of one or more other features, numbers, steps, actions, components, parts, or combinations thereof.

The antioxidant and moisturizing cosmetic composition (hereinafter referred to as'cosmetic composition') containing a plant extract according to an embodiment of the present invention contains a plant extract including an extract extracted from the above-ground part of gondalbi, rock brush, lemongrass, brier, It is possible to provide a cosmetic composition that more effectively exhibits antioxidant and moisturizing functions.

In addition, it is possible to provide a cosmetic composition that improves antioxidant function and exhibits anti-inflammatory effect by further including a botanical extract and the like in the plant extract.

Here, the gondalbi extract is an extract extracted from the above-ground part of the gondalbi, and has quercitrin, chlorogenic acid, and rutin as indicator materials, so it can exhibit antioxidant functions, and has the ability to generate hyaluronic acid to moisturize the skin. It can exert a moisturizing effect by expressing a factor related to.

In addition, the rock brush extract is an extract extracted from the above-ground part of the rock brush, and it has gallic acid and epigallocatechin gallate (EGCG) as indicator substances as indicator materials, so it can superficially reduce active oxygen through antioxidant effect. It has the ability to produce hyaluronic acid, so it can exhibit a moisturizing effect.

In addition, lemongrass extract is an extract extracted from the upper part of lemongrass, has hyaluronic acid production ability, has chlorogenic acid and p-Coumaric acid, which are related to skin moisturizing HAS1, HAS2, By expressing HAS3 and AQP3, it can control dryness, filaggrin and loricrin related to the skin barrier, and expression of PPAR-α, which is involved in skin moisturizing and epidermal differentiation regulation, can be increased.

In addition, the brier extract is an extract extracted from the brier flower, and has astragalin and gallic acid as indicator materials, so it can exhibit excellent antioxidant effects, and has the ability to generate hyaluronic acid, so that it can exhibit a moisturizing effect. At this time, the brier extract can improve the preservability of the cosmetic composition by exhibiting anti-oxidation and moisturizing functions as well as an antiseptic effect.

The present invention may exhibit more excellent antioxidant and moisturizing effects by containing a plant extract containing such an extract.

These gondalbi extract, rock brush extract, lemongrass extract, and brier extract can be prepared by extracting water or an organic solvent as a solvent, respectively.

The gondalbi, rock brush, lemongrass and brier used at this time are resource plants in the Namwon and Jirisan regions, and can be dried and used, which were collected from Namwon-si, Jeollabuk-do, and dried for 24 to 72 hours at 35 to 50°C, and can be used at 42°C. It is preferable to use one dried for 48 hours.

At this time, if the drying temperature is less than 35°C, the drying time may take too long or water evaporation is not well performed, so extraction efficiency may decrease. If the drying temperature exceeds 50°C, burning or destruction or deformation of the active ingredient may occur. .

In addition, when the drying time is less than 24 hours, drying is not performed well, and when the drying time exceeds 72 hours, since drying is sufficiently performed, it is inefficient, and the extraction efficiency may be lowered.

The organic solvent is preferably ethanol or 1,3-butylene glycol, but methanol, isopropanol, butanol, and the like may also be used. In addition, when using ethanol, it is preferable to use 50% ethanol, but this is only an example of the present invention, so it is not limited thereto.

More specifically, the gondalbi extract or rock brush extract or lemongrass extract or brier extract is added and heated 90 to 110 parts by weight of water or an organic solvent to 4 to 6 parts by weight of Eonryo (one of gondalbi, rock brush, lemon grass and brier) Then, it can be concentrated and freeze-dried to prepare. At this time, it is preferable to add 100 parts by weight of water or an organic solvent to 5 parts by weight of the raw material.

In this case, if the raw material is less than 4 parts by weight, the efficacy of the extract appears less, and if it exceeds 6 parts by weight, it may affect the scent and color, which are the organoleptic properties of the cosmetic.

In addition, if the solvent is less than 90 parts by weight, the active ingredient is not completely leached and the extract is concentrated even if only slightly evaporated, and the active ingredient in the extract may be locally destroyed by excessive heat. If the amount exceeds 110 parts by weight, the amount of the active ingredient is increased. It can be done, but it is inefficient when lyophilized.

On the other hand, when water is used as a solvent, it can be heated at 95 to 105°C for 230 to 250 minutes during extraction, and when using an organic solvent, it can be heated at 75 to 85°C for 230 to 250 minutes at the time of extraction, preferably When using water, it can be heated at 100°C for 240 minutes, and when using an organic solvent at 80°C for 240 minutes.

At this time, when the temperature is 95℃, the time is 230 minutes, when using water, the active ingredient may not be completely extracted if the temperature is less than 75℃ and 230 minutes when using an organic solvent. When using water, the temperature is 105℃ and the time is 250 When using a minute or organic solvent, if it exceeds 85°C and 250 minutes, the active ingredient may be destroyed, and since the yield of the active ingredient to be extracted no longer increases, further extraction is meaningless.

Gondalbee extract, rock brush extract, lemongrass extract, and brier extract can be extracted using either water or an organic solvent as a solvent as described above, but considering the degree of hyaluronic acid production ability, skin moisturizing and preservative effect, an organic solvent is used as a solvent. It is more preferable to use as to be extracted.

Plant extracts can be prepared by blending the extracts of Gondalbi extract, rock brush extract, lemongrass extract, and brier extract extracted as described above, and by mixing each extract at a desirable blending ratio, it has more effective anti-oxidation and moisturizing functions while still having an antiseptic effect. A cosmetic composition can be obtained.

That is, the plant extract including the gondalbi extract, the rock brush extract, the lemongrass extract and the brier extract may contain each extract in a weight ratio of 1 to 1.5: 1.5 to 2: 1 to 1.5: 0.5 to 1, and 1.5: 2: It is more preferable to include it in a weight ratio of 1:0.5.

When included in such a weight ratio, it can exhibit the most desirable antioxidant and moisturizing functions while exhibiting an antiseptic effect.

In addition, the plant extract may be contained in an amount of 3 to 8% by weight based on the total weight of the cosmetic composition, and 5% by weight is most preferred.

At this time, if the amount of the mixed extract is less than 3% by weight, relative to the total weight of the cosmetic composition, the antioxidant, moisturizing and preservative effect is insignificant, and if it exceeds 8% by weight, skin safety problems may occur and sensory properties may be affected. have.

In addition, the plant extract of the cosmetic composition according to the embodiment of the present invention may further include a barley bangle extract so as to improve the antioxidant function and also exhibit an anti-inflammatory effect.

Barley Bangi Extract is an extract extracted from the above-ground part of barley bangle. It has saponin, so it acts as an antioxidant and has anti-inflammatory effects such as soothing inflammation. It is extracted by using either water or an organic solvent as a solvent. It can be prepared as, the extraction conditions can be extracted under the same conditions as the other extracts.

Such a barley bangi extract may be further included in an amount of 1.5 to 2.5 parts by weight in the plant extract.

At this time, when the amount of the barley bangle extract is less than 1.5 parts by weight, the antioxidant enhancement and anti-inflammatory effects are insufficient, and when it exceeds 2.5 parts by weight, a problem may occur in skin safety.

That is, the plant extract may include a gondalbi extract, a rock brush extract, a lemongrass extract, a brier extract, and a barley pinch extract in a weight ratio of 1 to 1.5: 1.5 to 2: 1 to 1.5: 0.5 to 1: 0.1 to 0.5, and 1.5 It is more preferable to include in a weight ratio of: 1.5: 1: 0.5: 0.5.

In addition, the mixed extract of the cosmetic composition according to an embodiment of the present invention may further include sapodilla extract and peppermint fermented liquid to improve antioxidant function and preservation.

Sapodilla extract is an extract extracted from the fruit of Sapodilla. It has anti-aging effect by removing free radicals. It contains vitamin A to help maintain healthy mucous membranes and skin. It contains tannins, a polyphenol, to prevent inflammation. To help dermatitis with an action and soothing effect, it can be prepared by extracting the dried sapodilla fruit using either water or an organic solvent as a solvent, and the extraction conditions can be extracted under the same conditions as the other extracts. .

Peppermint fermentation broth has antioxidant and antibacterial effects, reduces evaporation of moisture, and improves the moisturizing effect, and improves the preservability of cosmetic compositions. A mixture of peppermint extract with xylitol powder, moringa powder, and cornelian milk powder. After fermentation by adding lactic acid bacteria can be prepared.

The peppermint extract used at this time is effective in alleviating allergic symptoms because it contains peppermint antioxidants and has an anti-inflammatory effect. It can be prepared by extracting dried peppermint using either water or an organic solvent as a solvent. Conditions can be extracted under the same conditions as the other extracts.

In addition, xylitol powder reduces evaporation of moisture and may benefit skin health.

In addition, moringa powder and cornus powder can improve the preservability of cosmetic compositions through antibacterial and purifying effects.

The lactic acid bacteria are preferably Bifidobacterium bifidum and Streptococcus thermophilus, but are not limited thereto.

Specifically, mint fermentation broth is prepared by mixing 20 to 30 parts by weight of xylitol powder, 0.1 to 0.5 parts by weight of moringa powder, 0.1 to 1 part by weight of cornus powder, and 0.1 to 1 part by weight of cornflower powder to 100 parts by weight of peppermint extract, and then adding lactic acid bacteria and naturally fermenting at room temperature. Can be. After that, it can be filtered and used.

At this time, if the xylitol powder is less than 20 parts by weight, the moisture evaporation prevention effect may be insignificant, and if it exceeds 30 parts by weight, the sensory properties of the cosmetic composition may be affected.

If the moringa powder is less than 0.1 parts by weight, the efficacy of the moringa powder is insignificant, and if it exceeds 0.5 parts by weight, there may be a problem in skin safety.

If the amount of cornus oil powder is less than 0.1 part by weight, the efficacy of the cornus oil powder is insignificant, and when it exceeds 1 part by weight, skin safety problems may occur and the sensory properties of the cosmetic composition may be affected.

The thus prepared peppermint fermentation broth is prepared by fermentation with lactic acid bacteria, so that the efficacy of each material is more excellent, so that it can effectively act on improving the function of the cosmetic composition.

The sapodilla extract and peppermint fermentation broth may be further included in the mixed extract in an amount of 2 to 2.5 parts by weight and 0.5 to 1 parts by weight, respectively.

In this case, when the sapodilla extract and the peppermint fermentation broth are each less than the above range, the synergistic effect such as antioxidant function and the effect of improving preservation appearing as they are further included are insignificant, and when the sapodilla extract and the peppermint fermentation broth are each less than the above range, the skin safety problem may occur.

That is, the plant extract is 1 to 1.5: 1.5 to 2: 1 to 1.5: 0.5 to 1: 0.1 to 0.5: 0.1 To 0.5: may be included in a weight ratio of 0.1 to 0.5, it is more preferably included in a weight ratio of 1: 1.5: 1: 0.5: 0.4: 0.4: 0.2.

Antioxidant and moisturizing cosmetic compositions containing such plant extracts include emulsions, creams, lotions, essences, packs, suspensions, pastes, gels, powders, lipsticks, makeup bases, foundations, lotions, ointments, gels, patches, essences, It may be formed as a cleansing foam, cleansing cream, cleansing water, soap, shampoo, conditioner, spray formulation, but is not limited thereto.

As described above, the antioxidant and moisturizing cosmetic composition containing the plant extract according to the embodiment of the present invention contains a plant extract including extracts extracted from the above-ground parts of gondalbi, rock brush, lemongrass, and brier, thereby antioxidant and moisturizing. The function can be expressed more effectively.

In addition, it not only has excellent antioxidant and moisturizing functions, but also can exhibit an antiseptic effect.

Hereinafter, the experimental examples to be carried out are provided to aid in understanding, and are merely illustrative of the present invention, and the scope of the present invention is not limited to the following experimental examples.

In addition, in order to find out the effect of the cosmetic composition, the Examples and Experimental Examples were described using ethanol as a solvent, but when preparing the cosmetic composition, 1,3-butylene glycol Can be used mainly.

[ Manufacturing example ]

[Preparation Example 1] Preparation of gondalbi extract

100 parts by weight of 50% ethanol was added to 5 parts by weight of dried gondal ratio, heated at 80° C. for 4 hours, concentrated, and freeze-dried to prepare.

[Preparation Example 2] Preparation of rock brush extract

100 parts by weight of 50% ethanol was added to 5 parts by weight of dried rock brush, heated at 80° C. for 4 hours, concentrated, and freeze-dried to prepare.

[Preparation Example 3] Preparation of lemongrass extract

100 parts by weight of 50% ethanol was added to 5 parts by weight of dried lemongrass, heated at 80° C. for 4 hours, concentrated, and freeze-dried to prepare.

[Preparation Example 4] Preparation of brier extract

100 parts by weight of 50% ethanol was added to 5 parts by weight of dried briers, heated at 80° C. for 4 hours, concentrated, and freeze-dried to prepare.

[Preparation Example 5] Preparation of barley bangle extract

100 parts by weight of 50% ethanol was added to 5 parts by weight of dried barley bangle, heated at 80° C. for 4 hours, concentrated, and freeze-dried to prepare.

[Preparation Example 6] Preparation of sapodilla extract

100 parts by weight of 50% ethanol was added to 5 parts by weight of dried sapodilla, heated at 80° C. for 4 hours, concentrated, and freeze-dried to prepare.

[Preparation Example 7] Preparation of peppermint fermentation broth

100 parts by weight of peppermint extract was prepared by mixing 20 parts by weight of xylitol powder, 0.5 parts by weight of moringa powder, and 1 part by weight of cornus powder, followed by addition of lactic acid bacteria and natural fermentation at room temperature.

[ Experimental example 1] polyphenol, flavonoid content and Phenolic compounds analysis

In order to confirm whether it has an antioxidant function and to confirm the phenolic compound of the extract, the contents of the polyphenols and flavonoids and the phenolic compound of Preparation Examples 1 to 4 were measured by the following method.

For polyphenols, 80 µl of Preparation Examples 1 to 4 and 20 µl of 1N Folin reagent were added to a 96 well plate, incubated for 5 minutes at room temperature, 100 µl of 2% Na2CO3 reagent was added, and then incubated for 30 minutes at room temperature. , ELISA absorbance was measured at 750nm.

For flavonoids, 10 μl of Preparation Examples 1 to 4 and 100 μl of diethylene glycol reagent were added to a 96-well plate, 10 μl of 1N NaOH was added, and then incubated at 37° C. for 1 hour, and the ELISA absorbance was measured at 420 nm.

For the analysis of phenolic compounds, 10 mg of Preparation Examples 1 to 4 or 1 mg of a standard product were taken and dissolved in 1 ml of 50% ethanol, and 10 μl of Preparation Examples 1 to 4 were injected into HPLC and analyzed at a rate of 0.8 ml/min.

Water (0.1% formic acid) and acetonitrile (0.1% formic acid) were used as the mobile phase, and the absorbance was used as a column using Shim-pack VP-ODS (4.6 ㎜ x 250 ㎜, 5 ㎛) (Shimadzu, Kyoto, Japan). It was analyzed at 280 nm.

The 19 standard products are gallic acid, gallic acid, chlorogenic acid, methyl gallate, caffeic acid, epigallocatein gallate (EGCG), and syringic acid. ), p-coumaric acid, rutin, ellagic acid, ferulic acid, astragalin, quercitrin, rosmarinic acid, Myricetin, luteolin, quercetin, apigenin, campferol and hesperetin (Sigma-aldrich, St. Louis, MO, USA) were used. I did. Table 1 shows the retention time and maximum absorption wavelength of 19 phenolic compounds.

The results are shown in Table 2 below.

As can be seen from Table 2, it was confirmed that all of Preparation Examples 1 to 4 contained polyphenols and flavonoids.

Accordingly, it was determined that all of the gondalbi extract, rock brush extract, lemongrass extract, and brier extract of Preparation Examples 1 to 4 could exhibit an antioxidant function.

In addition, Preparation Example 1 (Gondalbi extract) contains quercitrin, chlorogenic acid and rutin, and Preparation Example 2 (rock sol extract) is gallic acid and epigallocatechin gallate ( EGCG), Preparation Example 3 (lemongrass extract) contained chlorogenic acid and p-coumaric acid, Preparation Example 4 (brier extract) was Astragalin and Gallic acid. acid).

[ Experimental example 2] Antioxidant function

In order to confirm the antioxidant function, free radical scavenging activity (SC) was measured for the extracts of Preparation Examples 1 to 4 in the following manner to evaluate the antioxidant function.

DPPH (1,1-diphenyl-2-picrylhydrazyl radical) solution (60 M) was prepared. Then, the concentration of Examples 1 to 3 was adjusted to 1%, and the DPPH solution was mixed in a 1:1 ratio, followed by vortexing (vortex mixing). After reacting at room temperature for 30 minutes, absorbance was measured at 520 nm to obtain free radical scavenging activity (SC) according to the formula of Equation 1 below.

Ascorbic acid was used as a positive control.

[Equation 1]

Free radical scavenging ability (%) = (control absorbance-Example absorbance) / (control absorbance) X100

The results are shown in Table 3 below.

As can be seen from Table 3, it was confirmed that both Preparation Examples 1 to 4 showed higher free radical scavenging ability than the control group.

[ Experimental example 3] Moisturizing function

In order to confirm the moisturizing effect, the moisturizing function was analyzed by measuring hyaluronic acid secretion ability for the extracts of Preparation Examples 1 to 4 in the following manner.

Hyaluronic acid secretion ability was determined by using DMEM (High glucose, Welgene, Daegu, Korea) containing heat inactivation 10% fetal bovine serum (Merck, MA, USA) and 1% penicillin/streptomycin (Gibco, Carlsbad, CA, USA). 5% CO _{2 It} was cultured in a 37 ℃ cell incubator. Each sample was treated at a concentration of 50 μg/mL for 48 hours, and then measured by HA ELISA.

ATRA was used as a brewing control group.

The results are shown in Table 4 below.

As can be seen from Table 4, it was confirmed that the hyaluronic acid secretion ability appeared in all of Preparation Examples 1 to 4, and it was confirmed that Preparation Example 3 appeared higher than that of the control group.

[ Example 1, 2, Comparative example 1 to 4] Preparation of plant extracts

Preparation Examples 1 to 7 were mixed according to the weight ratio of Table 5 to prepare Examples 1 to 3 and Comparative Examples 1 to 4.

[ Formulation example 1 to 7] Cream preparation

Formulation Examples 1 to 7 as cosmetic compositions were prepared using the plant extracts of Examples 1 to 3 and Comparative Examples 1 to 4, respectively, and are shown in Table 6 below.

[ Experimental example 4] Antioxidant effect check

In order to confirm the antioxidant effect of Formulation Examples 1 to 7 in which cosmetic compositions were prepared using Examples 1 to 3 and Comparative Examples 1 to 4, other antioxidants, that is, BHT were compared as a sample, and the antioxidant was used using the NBT method The activity was measured. In order to measure the antioxidant effect, active oxygen produced by xanthine and xanthine oxidase was measured by the NBT method, and the effect of removing active oxygen from the test substance, that is, the active oxygen scavenging effect, was evaluated. The active oxygen scavenging rate was measured by reacting the active oxygen produced by xanthine and xanthine oxidase with Nitro Blue Tetrazolium (NBT) and measuring the blue produced by this at a wavelength of 560 nm. The method of measuring the active oxygen scavenging rate is as follows. To a vial (5 ml vial), _{2.4 ml of 0.05M Na 2} CO ₃ , 0.1 ml of 3 mM xanthine solution, 0.1 ml of 3 mM EDTA solution, 0.1 ml of BSA solution and 0.1 ml of 0.72 mM NBT solution were added, and DMSO (dimethyl sulfoxide) was added thereto. 0.1 ml of the sample solution prepared at a concentration of 0.1 (wt/v)% is added and left at 25°C for 10 minutes. Thereafter, 0.1 ml of a xanthine oxidase solution was added, stirred quickly, and incubation was started at 25°C for 20 minutes. Thereafter, _{0.1 ml of a 6Mm CuCl 2} solution was added to stop the reaction, and the absorbance St at 560 nm was measured. In the blank test, distilled water was used instead of the sample solution, and the absorbance Bt was measured in the same manner as above. Also, the blank of the sample solution is similarly operated using distilled water instead of the xanthine oxidase solution to measure the absorbance Bo. The result of the effect was calculated by the following formula, and the results are shown in Table 7 below.

Inhibition rate (%) = 〔1-([0039] St-So)/(Bt-Bo)〕Х 100

St: absorbance at 560 nm after enzymatic reaction of sample solution

Bt: absorbance at 560 nm after enzymatic reaction of blank test solution

So: Absorbance at 560 nm before reaction when no enzyme is added to the sample solution

Bo: Absorbance at 560 nm before reaction when no enzyme is added to the blank test solution

As can be seen from Table 7 above, it was possible to confirm the antioxidant effect in all samples. However, it was confirmed that Formulation Examples 1 to 3 prepared using Examples 1 to 3 exhibited better antioxidant effect than Formulation Examples 4 to 7 prepared using Comparative Examples 1 to 4, and exhibited better antioxidant effect than BHT. Could.

[ Experimental example 5] Check the skin moisturizing effect

In order to confirm the skin moisturizing effect of Formulation Examples 1 to 7 in which cosmetic compositions were prepared using Examples 1 to 3 and Comparative Examples 1 to 4, 90 healthy women aged 30 to 50 were tested.

In order to confirm the effect of improving skin moisturization, Formulation Examples 1 to 7 were divided into 10 people and applied twice a day (morning and evening) to the face for 2 weeks.

Before the experiment begins, determine the 2 areas to be measured, measure the moisture content of the skin 3 times, apply for 2 weeks (2 times/day), and then use the skin moisture content measuring device to use the epidermal layer just below the stratum corneum. The moisture of the was measured. The measurement results are shown in Table 8.

As can be seen from Table 8 above, the moisture content was increased in all samples, thereby confirming the moisturizing effect. However, it was confirmed that Formulation Examples 1 to 3 prepared using Examples 1 to 3 exhibited better moisturizing effect than Formulation Examples 4 to 7 prepared using Comparative Examples 1 to 4.

[ Experimental example 6] Preservation evaluation

Formulation Examples 1 to 5 were stored at a constant temperature and humidity (high temperature, 40°C, and the pH change was measured periodically for 10 weeks to investigate the preservability of each formulation example, and the results are shown in Table 9).

As can be seen from Table 9, it was confirmed that the pH change of Formulation Examples 1 to 3 containing the brier extract had less pH change than Formulation Examples 4 and 5, and the pH change of Formulation Example 3 to which the peppermint fermentation broth was added was the least. I could confirm that.

Accordingly, it is judged that the formulation Examples 1 to 3 have superior preservation properties than Formulation Examples 4 and 5, and Formulation Example 3 is judged to have the best preservation properties.

The embodiments of the present invention have been described above with reference to the accompanying drawings, but those of ordinary skill in the art to which the present invention pertains can be implemented in other specific forms without changing the technical spirit or essential features of the present invention. You will be able to understand. Therefore, the embodiments described above are illustrative and non-limiting in all respects.

Claims (11)

Antioxidant and moisturizing cosmetic composition containing plant extracts including gondalbi extract, rock brush extract, lemongrass extract and brier extract.
The method of claim 1,
The gondalbi extract,
Antioxidant and moisturizing cosmetic composition, characterized in that it has quercitrin, chlorogenic acid, and rutin as indicator substances.
The method of claim 1,
The rock brush extract,
Antioxidant and moisturizing cosmetic composition, characterized in that it has gallic acid and epigallocatechin gallate (EGCG) as indicator substances.
The method of claim 1,
The lemongrass extract,
Antioxidant and moisturizing cosmetic composition, characterized in that it has chlorogenic acid and p-coumaric acid as indicator substances.
The method of claim 1,
The brier extract,
Antioxidant and moisturizing cosmetic composition, characterized in that it has astragalin (Astragalin) and gallic acid (Gallic acid) as indicator substances.
The method of claim 1,
The plant extract,
Antioxidant and moisturizing cosmetic composition, characterized in that each extract is extracted using water or an organic solvent as a solvent.
The method of claim 1,
The plant extract,
Antioxidant and moisturizing cosmetic composition comprising the gondalbi extract, rock brush extract, lemongrass extract, and brier extract in a weight ratio of 1 to 1.5: 1.5 to 2: 1 to 1.5: 0.5 to 1.
The method of claim 1,
The plant extract,
Antioxidant and moisturizing cosmetic composition, characterized in that it is contained in an amount of 3 to 8% by weight, based on the total weight of the cosmetic composition.
The method of claim 1,
The plant extract,
Antioxidant and moisturizing cosmetic composition further comprising a barley bangi extract.
The method of claim 1,
The plant extract,
Antioxidant and moisturizing cosmetic composition further comprising sapodilla extract and peppermint fermentation liquid.
The method of claim 1,
Emulsion, cream, lotion, essence, pack, suspension, paste, gel, powder, lipstick, makeup base, foundation, lotion, ointment, gel, patch, essence, cleansing foam, cleansing cream, cleansing water, soap, shampoo, rinse , Antioxidant and moisturizing cosmetic composition, characterized in that the formulation of a spray.