KR20210035483A - Composition for cultivating strawberry using Spirulina - Google Patents

Abstract

The present invention relates to a nutrient or fertilizer composition for strawberry cultivation using spirulina. More particularly, the present invention includes a fermented extract of spirulina as an active ingredient, wherein the fermented extract of spirulina is obtained by fermenting a spirulina extract with a Bacillus strain. According to the present invention, the composition has an advantage of promoting strawberry growth, having high contents of choline, vitamins, and calcium of strawberry, and cultivating strawberry with high sugar content.

Description

Composition for nutrient or fertilizer for cultivating strawberries using spirulina {Composition for cultivating strawberry using Spirulina}

The present invention relates to a nutrient or fertilizer composition for strawberry cultivation using spirulina, and more particularly, by using a fermented extract of spirulina as a nutrient or fertilizer composition for strawberry cultivation, promoting the growth of strawberries, calcium, vitamin, choline, etc. It relates to a nutrient or fertilizer composition for cultivating strawberries using spirulina that increases the content of and increases the sugar content.

Strawberry (Fragaric ananassa Duch) is a perennial plant belonging to the Rosaceae family. It has excellent scent and color, is well harmonized with acidity and sweetness, and has a unique scent. It has been used habitually in spring for a long time. In particular, the content of vitamin C is higher than that of other fruits, and it has been in the spotlight for reproduction. Strawberries have different ingredients and contents depending on the variety, but in general, they have a lot of organic acids, so they have a lot of acidity and a lot of sugar, and vitamins such as vitamin C are contained in a large amount compared to other fruits and vegetables.

In particular, strawberries contain various antioxidants such as quercetin, caffeic acid, ferulic acid, and flavonols, as well as free and bound phenolic compounds and anthocyanins. It is also known to exhibit antioxidant activity and proliferation inhibitory effect on HepG2 cells, which are human liver cancer cells.

These strawberries are eaten raw when fresh, or eaten as salads and sandwiches, and are processed into processed foods such as jam, jelly, ice cream, frozen strawberries, syrup, juice, and processed milk products (strawberry milk).

In recent years, it is gaining popularity as a well-being food containing a large amount of various components useful for the human body, and accordingly, various researches and developments are in progress.

For example, Korean Patent Laid-Open No. 10-2004-0052747 cultivation of strawberries containing selenium and a method for producing enzymes, Korean Patent Laid-Open Patent No. 10-2004-0052748 cultivation of strawberries containing germanium, and a method for producing enzymes , Korean Patent Registration No. 10-0970840, a natural fermentation composition for strawberry cultivation, and a method of cultivating strawberries having a high choline content using the same.

However, the above Patent Publication Nos. 10-2004-0052747 and 10-2004-0052748 are not grown using natural materials, but the soil for growing strawberries by cultivating strawberries using metal minerals such as selenium or germanium. There is a problem that may cause contamination of the strawberry, as well as a problem that the growth of strawberries may be degraded, and there is a problem that economical cost is high as a metal inorganic material purified by a chemical method is used.

In addition, the Patent No. 10-0970840 has the advantage of cultivating strawberries having a high choline content without causing contamination of the soil, but there is a disadvantage in that the growth promotion of strawberries is not sufficient.

On the other hand, Spirulina is the first photosynthetic organism that was born 3.5 billion years ago, and its name is derived from the etymology of "a spiral-shaped microorganism". In 1827, the German oceanographer Turpin first isolated Spirulina from freshwater, and it is called spirulina. Named it. Spirulina was used as a food for the indigenous peoples living around the lakes where they lived, and in the 16th century it was also used as a staple food for the Aztec Mayans.

Spirulina is a high-density natural chlorophyll nutrient rich in all nutrients needed by living organisms, especially protein and green and yellow vegetables. It contains ~30,000 kinds of even nutrients. In addition, it has a very high protein content (55 ~ 77%), and various components are composed of almost the same ratio as the human body, so the digestive absorption rate in the body is more than 95%, and can be consumed by patients with weakened digestive function, the elderly, and infants. It is a strong alkalinity complete food that has great effect and does not cause an imbalance in nutrition even if taken for a long period of time. In particular, it has been officially recognized by the United Nations (UN), the World Health Organization (WHO), and the United States Food and Drug Administration (FDA) as a complete food in terms of safety and nutrition as a health food. It is also a decided food.

This spirulina has limited use, and has not been used as a composition for cultivating strawberries anywhere in the past.

KR 10-2004-0052747 A KR 10-2004-0052748 A KR 10-0970840 B1

Accordingly, an object of the present invention is to provide a nutrient or fertilizer composition for strawberry cultivation using Spirulina which promotes the growth of strawberries, increases the content of choline, vitamins, and calcium, and increases sugar content without soil contamination.

The nutrient or fertilizer composition for cultivating strawberries using spirulina of the present invention for achieving the above object comprises a fermented extract of spirulina as an active ingredient, and the fermented extract of spirulina is characterized in that the extract of spirulina is fermented with a Bacillus strain. It is done.

The fermented extract of Spirulina includes drying and pulverizing spirulina, sterilizing the dried spirulina pulverized product, and inoculating Bacillus subtilis TP6 to the sterilized spirulina pulverized product, and a fermentor Fermenting the fermented spirulina for 1 to 5 days at a temperature of 30 to 37°C, and adding at least one extraction solvent of 10 to 20 times by weight of water and ethanol to the fermented spirulina and extracting for 5 to 10 days And, filtering, concentrating and drying the extracted extract.

As the active ingredient, further comprising a fermented extract of mosae tree fruit and a fermented extract of oleum tree leaves, wherein the fermented extract of mosae tree fruit is extracted by adding hot water of 50 to 100° C. to the dried product of Mosae tree fruit Wow, prepared by inoculating Bacillus subtilis TP6 in the extract and fermenting for 1 to 5 days at a temperature of 30 to 37°C in a fermentor, and fermentation of the leaves The extract is extracted by adding hot water of 50 to 100°C to the dried leaves of the serrata, and inoculating Bacillus subtilis TP6 to the extract, and at a temperature of 30 to 37°C in a fermentor. It characterized in that it is produced, including the step of fermenting for 1 to 5 days.

As the active ingredient, further comprising a fermented extract of radish, wherein the fermented extract of radish is extracted by adding hot water of 50 to 100°C to dried radish, and Bacillus subtilis TP6 (Bacillus subtilis) to the extract. It is characterized in that it is prepared by inoculating TP6) and fermenting for 1 to 5 days at a temperature of 30 to 37°C in a fermentor.

As the active ingredient, with respect to 100 parts by weight of the fermented extract of Spirulina, 10 to 20 parts by weight of the fermented extract of the Mosae tree fruit, 10 to 20 parts by weight of the fermented extract of the leaves of the serrata, and 10 to 10 parts by weight of the fermented extract of the radish It contains 20 parts by weight, and the composition is characterized in that it is a nutrient or fertilizer.

According to the nutrient or fertilizer composition for cultivating strawberries using Spirulina of the present invention, as well as promoting the growth of strawberries, there is an advantage in that the strawberry has a high content of choline, vitamins, and calcium, and it is possible to cultivate strawberries having excellent sugar content.

Hereinafter, the present invention will be described in detail.

The greatest feature of the present invention is to promote the growth of strawberries, increase the content of choline, vitamins, and calcium, and increase the sugar content by using a composition containing the fermented extract of Spirulina as an active ingredient as a nutrient or fertilizer.

That is, the composition for cultivating strawberries using spirulina according to the present invention is characterized in that it contains a fermented extract of spirulina as an active ingredient. At this time, the fermented extract of Spirulina is characterized in that it is extracted after fermenting Spirulina into a Bacillus strain.

The fermented extract of Spirulina increases the physiological activity of various nutrients by various enzymes produced by the fermentation strain during fermentation, thereby improving its functionality. It has the characteristic of increasing the sugar content while doubling the ingredients.

In the present invention, the spirulina includes various known spirulina species, and illustratively Spirulina platensis, Spirulina maxima, Spirulina subsalsa, Spirulina major, Spirulina geitleri, Spirulina siamese, Spirulina princeps, Spirulina laxissima, Spirulina curta, Spirulina spirulinoides, Spirulina platensis plate , Spirulina maxima, Spirulina geitleri and Spirulina siamese may be selected from, but are not limited to.

The spirulina contains proteins, carbohydrates, water-soluble dietary fiber, antioxidant pigment components (chlorophyll, carotenoids, phycocyanin), antioxidant enzymes (SOD), and the like, and vitamins include beta-carotene, vitamins B1, B2, B6, B12 , Vitamin E, inositol, folic acid, as well as a large amount of choline. In addition, it contains a large amount of inorganic components such as calcium, iron, potassium, magnesium, zinc, manganese, selenium, and germanium. It contains all essential amino acids, and is rich in essential fatty acids such as linolenic acid and gamma linolenic acid. Therefore, when the spirulina is fermented and extracted, its nutritional components are doubled, and when it is applied to strawberry cultivation, various nutritional components can be doubled.

The fermented extract of Spirulina includes drying and pulverizing spirulina, sterilizing the dried spirulina pulverized product, and inoculating Bacillus subtilis TP6 to the sterilized spirulina pulverized product, and a fermentor Fermenting the fermented spirulina for 1 to 5 days at a temperature of 30 to 37°C, and adding at least one extraction solvent of 10 to 20 times by weight of water and ethanol to the fermented spirulina and extracting for 5 to 10 days And, filtering, concentrating and drying the extracted extract.

More specifically, spirulina is dried at 20 to 40° C. and pulverized with 100 to 200 mesh. And it is mixed with 1 to 5 times by weight of purified water, and sterilized for 10 to 20 minutes at a temperature range of 120 to 125°C. It is to prevent invasion of other contaminants other than the fermentation strain during fermentation through the above sterilization.

And after the sterilization, the sterilized spirulina pulverized product is inoculated with a fermentation strain of Bacillus strain, most preferably Bacillus subtilis TP6, and fermented at a temperature of 30 to 37°C for 1 to 5 days. . The reason for inoculating Bacillus subtilis TP6 in the present invention is that the active ingredient of Spirulina can be further doubled compared to other fermented strains.

And the fermented spirulina was dried for 12 to 24 hours at a temperature range of 40 to 50°C to obtain a solid fermented spirulina, and the solid fermented spirulina was mixed with at least one extraction solvent of 10 to 20 times by weight of water and ethanol Mix and extract with stirring at 30-100° C. for 5-10 days. Next, the extract is filtered, concentrated, and freeze-dried to obtain a spirulina fermented extract.

The composition containing the fermented extract of Spirulina prepared as described above, when used as a nutrient or fertilizer for strawberry cultivation as described above, promotes the growth of strawberries, promotes the growth of strawberries, and doubles various nutrients while It has the effect of increasing the sugar content.

On the other hand, the nutrient or fertilizer composition for cultivation of strawberries may further include a fermented extract of a mosae tree fruit and a fermented extract of a leaf of a wild bodhi tree.

The fermented extract of the mosae tree fruit and the fermented extract of the leaves of the garden bodhi tree promote the growth of strawberries and further increase the sugar content.

Here, the fermented extract of the mosae tree fruit is added 10 to 20 times by weight of hot water at 50 to 100° C. to the dried product of the moss tree fruit and extracted for 10 to 20 hours, and then filtered, and Bacillus subtilis is added to the filtered extract. It can be prepared by inoculating TP6 (Bacillus subtilis TP6) and fermenting for 1 to 5 days at a temperature of 30 to 37°C in a fermentor. In addition, it is natural that the fermented product can be filtered, concentrated, and dried.

In addition, the fermented extract of the leaves of the Tsukibori leaves was extracted for 10 to 20 hours by adding hot water of 50 to 100°C of 10 to 20 times by weight to the dried product of the leaves of the Tsukibori, and then filtered, and Bacillus subs the filtered extract. It can be prepared by inoculating Tilis TP6 (Bacillus subtilis TP6) and fermenting for 1 to 5 days at a temperature of 30 to 37°C in a fermentor. In addition, it is natural that the fermented product can be filtered, concentrated, and dried.

In addition, the strawberry cultivation nutrient or fertilizer composition may further include a fermented extract of radish. The fermentation extraction of the radish enhances the nutrients of strawberries and promotes growth.

The fermented extract of radish was extracted for 10 to 20 hours by adding hot water of 10 to 20 weight times of 50 to 100°C to the dried radish, and then filtered, and Bacillus subtilis TP6 to the filtered extract. Can be prepared by inoculating and fermenting for 1 to 5 days at a temperature of 30 to 37° C. in a fermentor. In addition, it is natural that the fermented product can be filtered, concentrated, and dried.

In the present invention, when the fermented extract of spirulina, the fermented extract of mosquito fruit, the fermented extract of the leaves of the serrata, and the fermented extract of radish are included as active ingredients, the amount of each fermented extract is 100% by weight of the fermented extract of the spirulina. With respect to parts, 10 to 20 parts by weight of fermented extract of Mosaic tree fruit, 10 to 20 parts by weight of fermented extract of Tsukibori leaves, and 10 to 20 parts by weight of fermented extract of radish are preferred, which promotes strawberry growth and nutritional components. It takes into account the effect of increasing the sugar content and weight.

And since the composition for cultivating strawberries according to the present invention can be used as a nutrient or fertilizer, when used as a nutrient, the composition is diluted with water of 450 to 550 times, and the diluted strawberry cultivating composition is used for 5 to 10 days when flowering blooms. Foliar spray can be applied 2-3 times at intervals. At this time, it is effective to set the amount of foliar spraying to 1~3ℓ/pyeong as a diluted solution, but this is not limited. In addition, when used as a fertilizer, the composition may be diluted with water of 450 to 550 times, and the diluted strawberry cultivation composition may be applied to the soil at an amount of 1 to 3 ℓ/pyeong, but this is not limited thereto.

Hereinafter, the present invention will be described in more detail through specific examples.

(Example 1)

After collecting spirulina, it was dried at 25° C. for 24 hours, and then pulverized with 100 mesh. Three times the amount of purified water was added to the pulverized material, and sterilized at 121°C for 15 minutes. And the sterilized spirulina was inoculated with Bacillus subtilis TP6 10% (w/v). Next, the spirulina inoculated with the fermentation strain was put into a solid-state fermentor and fermented for 5 days at a temperature of 35°C. Then, the fermented spirulina was dried at a temperature of 40° C. for 24 hours to obtain a solid fermented product, and 15 weight times of 70% (v/v) ethanol at 40° C. was added to the solid fermented product, followed by stirring for 5 days. Extracted. Then, the extract was filtered through a 250 mesh filter, concentrated under reduced pressure at 60° C., and then freeze-dried to prepare a sample.

(Example 2)

In the same manner as in Example 1, a sample was prepared by mixing 200 g of a fermented extract of Mosae tree fruit and 200 g of a fermented extract of oleum tree leaf in 1 kg of the sample prepared as in Example 1.

Here, the fermented extract of the mosae tree fruit is dried at 25° C. for 24 hours and then pulverized with 100 mesh, 10 times by weight of 80° C. hot water is added to the pulverized product and extracted for 15 hours, and then it is used with a 250 mesh filter. Filtered. And after inoculating Bacillus subtilis TP6 2% (w/v) to the filtered extract, put it into a fermentor and fermented for 3 days at a temperature of 35° C., filtered again with a 250 mesh filter, and 60° C. It was prepared by concentrating under reduced pressure and freeze-dried.

And the fermented extract of the leaves of the Tsurubori leaves, dried at 25°C for 24 hours, pulverized them with 100 mesh, added 10 times by weight of hot water at 80℃ to the pulverized product and extracted for 15 hours, and then filtered with a 250 mesh filter. Filtered. And after inoculating Bacillus subtilis TP6 2% (w/v) to the filtered extract, put it into a fermentor and fermented for 3 days at a temperature of 35° C., filtered again with a 250 mesh filter, and 60° C. It was prepared by concentrating under reduced pressure and freeze-dried.

(Example 3)

In the same manner as in Example 2, a sample was prepared by mixing 200 g of a fermented extract of radish to 1.4 kg of a sample prepared as in Example 2.

At this time, the fermented extract of radish was dried radish at 25° C. for 24 hours and then pulverized with 100 mesh, 10 times by weight of 80° C. hot water was added to the pulverized product, extracted for 15 hours, and then filtered through a 250 mesh filter. And after inoculating Bacillus subtilis TP6 2% (w/v) to the filtered extract, put it into a fermentor and fermented for 3 days at a temperature of 35° C., filtered again with a 250 mesh filter, and 60° C. It was prepared by concentrating under reduced pressure and freeze-dried.

(Test Example 1)

When cultivating strawberries, each of the samples of Example 1, Example 2, and Example 3 was foliar sprayed three times at intervals of 10 days from the opening of the flower room. Each of the samples was diluted with 500 times of water and sprayed, and the amount of foliar spraying was 2 ℓ/pyeong.

The average weight of 100 strawberries grown and harvested in this way was measured, and the sugar content, calcium, vitamin, and choline content were measured.

The results are shown in Table 1 below.

Test Example 1 result Item No treatment Example 1 Example 2 Example 3 unit Test Methods Sugar content 10.3 12.1 12.7 12.7 Brix Refractometer calcium 13 151 155 171 mg/100g Micronutrient Test Method Vitamin C 82 97 105 121 mg/100g Micronutrient Test Method Choline 1.98 0.241 0.248 0.247 mg/100g Micronutrient Test Method weight 24.5 28.7 31.2 32.3 g

As can be seen in Table 1, as a result of cultivating strawberries by applying the composition of the present invention, the content of calcium, vitamin C, and choline increased as well as the sugar content increased and the weight increased compared to the untreated group. I was able to confirm that.

Above, the present invention has been described in detail using preferred embodiments, but the scope of the present invention is not limited to specific embodiments, and should be interpreted by the appended claims. In addition, those who have acquired ordinary knowledge in this technical field should understand that many modifications and variations are possible without departing from the scope of the present invention.

Claims (5)

Including a fermented extract of Spirulina as an active ingredient,
The fermented extract of Spirulina,
A composition for cultivating strawberries using spirulina, characterized in that the extract of Spirulina is fermented with a Bacillus strain.
The method of claim 1,
The fermented extract of Spirulina,
Drying and pulverizing spirulina, and
Sterilizing the dried spirulina pulverized product,
Inoculating Bacillus subtilis TP6 to the sterilized spirulina pulverized product, and fermenting for 1 to 5 days at a temperature of 30 to 37°C in a fermentor,
Injecting at least one extraction solvent of 10 to 20 times by weight of water and ethanol to the fermented Spirulina and extracting for 5 to 10 days,
A composition for cultivating strawberries using spirulina, comprising the steps of filtering, concentrating and drying the extracted extract.
The method according to claim 1 or 2,
As the active ingredient,
Further comprising a fermented extract of the Mosae tree fruit and a fermented extract of the leaves of the garden bodhi tree,
The fermented extract of the mosae tree fruit,
Extracting by adding hot water of 50 ~ 100 ℃ to the dried product of the mosae tree fruit,
It is prepared including the step of inoculating Bacillus subtilis TP6 to the extract and fermenting for 1 to 5 days at a temperature of 30 to 37°C in a fermentor,
The fermented extract of the leaves of the garden bodhi tree,
Extracting by adding hot water of 50 ~ 100 ℃ to the dried leaves of the garden bodhi tree,
Strawberry cultivation composition using spirulina, characterized in that it is prepared including the step of inoculating Bacillus subtilis TP6 to the extract and fermenting for 1 to 5 days at a temperature of 30 to 37°C in a fermentor .
The method of claim 3,
As the active ingredient,
Further comprising a fermented extract of the radish,
The fermented extract of radish,
Extracting the dried radish by adding hot water of 50 ~ 100 ℃,
Strawberry cultivation composition using spirulina, characterized in that it is prepared including the step of inoculating Bacillus subtilis TP6 to the extract and fermenting for 1 to 5 days at a temperature of 30 to 37°C in a fermentor .
The method of claim 4,
As the active ingredient,
With respect to 100 parts by weight of the fermented extract of Spirulina, 10 to 20 parts by weight of the fermented extract of the Mosae tree fruit, 10 to 20 parts by weight of the fermented extract of the leaves of the serrata, and 10 to 20 parts by weight of the fermented extract of radish
The composition is a strawberry cultivation composition using spirulina, characterized in that the nutrient or fertilizer.