KR20210033368A - Anti-Pollution Cosmetic Composition Comprising Salvia Plebeia Extract - Google Patents

Abstract

The present invention relates to an anti-pollution cosmetic composition comprising an extract extracted from Salvia plebeia, wherein the Salvia plebeia extract may have an anti-pollution effect against air pollutants that may be in contact with the skin by being captured in nanoliposomes.

Description

[Anti-Pollution Cosmetic Composition Comprising Salvia Plebeia Extract]

The present invention relates to an anti-pollution cosmetic composition comprising a gombo cabbage extract, and in more detail, it includes a gombo cabbage extract extracted from gombo cabbage, wherein the gombo cabbage extract is captured as nanoliposomes, in the atmosphere that can contact the skin It relates to an anti-pollution cosmetic composition comprising a gombo cabbage extract that can have an anti-pollution effect against contaminants.

As we enter the modern society, environmental pollution is becoming more severe and the influx of pollutants into the areas where people live is increasing. Due to the introduction of such pollutants, people get diseases such as lungs or bronchi, and problems of lowering the life expectancy are being revealed one after another.

The pollutants cause damage to the skin while contacting the skin as well as the lungs or bronchi, and adverse effects such as dermatitis, skin rash, and skin necrosis are exerted. Therefore, the need for cosmetics that can safely protect skin from pollutants is on the rise.

Benzopyrene is exemplified as belonging to the pollutant. The benzopyrene is produced due to incomplete combustion such as automobile exhaust gas or cigarette smoke, and is a toxic substance that causes cell damage through metabolism conversion to epoxide and reaction with DNA. Recently, as news that it causes atopic dermatitis is exposed, adverse effects on the skin are revealed, and it is one of the dangerous substances that can cause skin deterioration due to problems such as cell mutation and cell damage.

In addition, nitrogen oxides are among the pollutants. The nitrogen oxide is a compound composed of nitrogen and oxygen, and includes nitrogen monoxide, nitrogen dioxide, dinitrogen monoxide, dinitrogen trioxide, dinitrogen tetraoxide, dinitrogen pentoxide, and the like. In general, nitrogen gas is stable and it is difficult to split the atoms constituting it into two, but the bond may be broken when a very high temperature is applied. Often, a high temperature is formed inside the engine of a vehicle, and the exhaust gas of the vehicle contains a large amount of nitrogen oxides.

The nitrogen oxide may include ammonium or a salt thereof. Nitric acid group, another form of nitrogen oxide, reacts with water to form nitric acid and may cause acid rain. The nitric acid combines with ammonium, which is an alkaline substance in the atmosphere, to produce ammonium nitrate, which is a particulate matter in the atmosphere, which is a secondary fine dust and adversely affects the human body. When the ammonium nitrate contacts the human body, it causes inflammation in the eyes, nasal mucosa and skin.

In addition, formaldehyde is one that belongs to the pollutant. The formaldehyde is a colorless gas with a strong odor at room temperature and is also referred to as methanol. The formaldehyde is easily produced when a material containing carbon is incompletely burned, and is synthesized by applying sunlight and oxygen to hydrocarbons such as methane in the air. The formaldehyde is a skin allergy aid, and when it comes into contact with the skin, it may cause problems such as skin rash, skin, and dermatitis.

The above contaminants in contact with the skin cause inflammation and necrosis, and even if the remaining amount in the atmosphere is in contact, if continuous contact occurs, it causes skin troubles.Therefore, measures to deal with the contact of the contaminants with the skin should be devised. will be.

Previously, the trend was to only pay attention to the internal aging of the skin, or to only pay attention to ultraviolet rays as an external factor, but as fine dust and yellow dust become issues, the population interested in the deterioration of the skin's function against contaminants is increasing. to be.

Accordingly, research on cosmetics that can protect the skin against contaminants is continuing, but formulations using chemicals have been mainly studied, and there is a problem that chemicals can cause side effects on the skin and are difficult to sustain ecologically. there was.

Therefore, it is time to study on an anti-pollution cosmetic composition using natural ingredients that are ecologically sustainable and have few side effects.

Republic of Korea Patent Registration No. 10-1908975 (announced on October 17, 2018), "Anti-pollution cosmetic composition containing sawijil bread extract as an active ingredient" Republic of Korea Patent Registration No. 10-1944025 (announced on Jan. 30, 2019), "Anti-pollution cosmetic composition containing Haneultari extract as an active ingredient"

In order to solve the above problems, the present invention includes a gombo cabbage extract extracted from gombo cabbage, and the gombo cabbage extract is collected as nanoliposomes, so that it can have an anti-pollution effect against air pollutants that may come into contact with the skin. It is an object of the present invention to provide an anti-pollution cosmetic composition comprising a gombo cabbage extract.

In order to solve the above problems, the present invention provides an anti-pollution cosmetic composition comprising a gombo cabbage extract extracted from gombo cabbage, wherein the gombo cabbage extract is collected as nanoliposomes. .

In addition, the cosmetic composition is characterized in that it has an anti-pollution effect.

In addition, the gombo cabbage extract is characterized in that the gombo cabbage is extracted from the bath.

In addition, the nanoliposome is characterized in that the gombo cabbage extract is collected through a high pressure homogenizer.

In addition, the gombo cabbage extract is characterized in that it contains 0.1 to 30.0% by weight in the nanoliposome.

The anti-pollution cosmetic composition comprising the extract of Gombo cabbage according to the present invention includes the extract of Gombo cabbage extracted from Gombo cabbage, and the Gombo cabbage extract is collected as nanoliposomes to prevent airborne contaminants in contact with the skin. Has a pollution effect.

1 is an exemplary view showing a nanoliposome according to the present invention.
Figure 2 is a graph showing the anti-pollution effect on benzopyrene of an anti-pollution cosmetic composition comprising a gombo cabbage extract according to the present invention.
Figure 3 is a graph showing the anti-pollution effect on ammonium nitrate of an anti-pollution cosmetic composition comprising a gombo cabbage extract according to the present invention.
Figure 4 is a graph showing the anti-pollution effect on formaldehyde of the anti-pollution cosmetic composition comprising the extract of Gombo cabbage according to the present invention.

The following detailed descriptions of the present invention are embodiments in which the present invention may be practiced and refer to the accompanying drawings, which are illustrated as examples of the embodiments. These embodiments will be described in detail enough for those skilled in the art to practice the present invention. It should be understood that the various embodiments of the present invention are different from each other, but need not be mutually exclusive. For example, specific shapes, structures, and characteristics described herein may be implemented in other embodiments without departing from the spirit and scope of the present invention in relation to one embodiment. In addition, it should be understood that the location or arrangement of individual components in each described embodiment may be changed without departing from the spirit and scope of the present invention.

Accordingly, the detailed description to be described below is not intended to be taken in a limiting sense, and the scope of the present invention is limited only by the appended claims, along with all scopes equivalent to those claimed by the claims, if appropriately described. Like reference numerals in the drawings refer to the same or similar functions over several aspects.

Terms used in the present invention have selected general terms that are currently widely used as possible while taking functions of the present invention into consideration, but this may vary according to the intention or precedent of a technician working in the field, the emergence of new technologies, and the like. In addition, in certain cases, there are terms arbitrarily selected by the applicant, and in this case, the meaning of the terms will be described in detail in the description of the corresponding invention. Therefore, the terms used in the present invention should be defined based on the meaning of the term and the overall contents of the present invention, not a simple name of the term.

In the present invention, when a part "includes" a certain component, it means that other components may be further included rather than excluding other components unless otherwise stated.

Hereinafter, the anti-pollution cosmetic composition comprising the extract of Gombo cabbage according to the present invention will be described in detail.

The present invention, Gombo cabbage extract extracted from Gombo cabbage ( Salvia Plebeia Extract ), but the gombo cabbage extract is collected as a nano liposome, and an anti-pollution cosmetic comprising a gombo cabbage extract that can have an anti-pollution effect against air pollutants that may come into contact with the skin. The composition is provided.

The gombo cabbage ( Salvia Plebeia ) is a perennial plant belonging to the Lamiaceae family and is a weed that grows in rice fields or fields in various places in Korea. It grows about 15 to 90cm tall and has a lot of twigs. The stem is square and covered with soft hairs. Leaves are oval-shaped or lanceolate, 2-6cm long, 8-25mm wide, and the ends are dull or sharpened, with round sawtooth on the edge, and short and soft hairs on the leaf veins. Small light purple flowers are scattered at the ends of branches around June, and seeds ripen in July. The roots are cabbage roots, but there are many fine roots, and the outpost smells like fishy. Even in winter, the leaves do not dry out and spread widely in the shape of a rosette so they pass through winter. In winter, the shape of the leaves attached to the floor spreads like cabbage, but it is called Gombo cabbage because it is much smaller than the cabbage and the wrinkled shape of the leaves is called Gombo cabbage.

The gombo cabbage contains flavonoids, homoplantangiminin, hispidulin, eukaponoline, eukaponoline-7-glucoside, and the like. In addition, it contains phenolic substances, essential oils, saponins, cardiac glycosides, and unsaturated fatty acids.

The gombo cabbage extract refers to an extract obtained from gombo cabbage.

In more detail, the gombo cabbage extract is one or more selected from the group consisting of water, anhydrous or hydrated lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butylene glycol. It is appropriate to extract using an extraction solvent.

Alcohol is preferred, and ethanol is most appropriately used. The ethanol is preferably 50 to 90% of ethanol.

The appropriate weight of the extraction solvent is preferably 1 to 20 times the weight of the dry weight of the Gombo cabbage, and more preferably 10 times the weight.

The extraction of the gombo cabbage extract may be performed by a bath extraction method, and at this time, it is preferable to heat the temperature to 60 to 80°C, and it will be appropriate to perform the extraction for 1 to 24 hours. During the heating process, the flavonoids, homoplantangiminin, hispidulin, eukaponoline, eukaponoline-7-glucoside, phenolic substances, essential oils, saponins, cardiac glycosides and unsaturated fatty acids have changed during the heating process. It may be that the anti-pollution effect of the anti-pollution cosmetic composition comprising the extract of Gombo cabbage according to the present invention is increased.

The nanoliposome is collected in the extract of the gombo cabbage.

The collection of the nanoliposome may be prepared by a mixture containing a gombo cabbage extract, a polyol, a surfactant, a phospholipid, a fatty acid, and water.

The gombo cabbage extract is preferably contained in an amount of 0.1 to 30.0% by weight based on the total weight of the nanoliposome.

The polyol is not particularly limited, for example, selected from the group consisting of propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, methylpropanediol, isoprene glycol, pentylene glycol, erythritol, xylitol and sorbitol, etc. One or more may be used, preferably propylene glycol. The polyol is preferably 0.1 to 70% by weight based on the total weight of the nanoliposome.

As the surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant and nonionic surfactant may be used, and anionic surfactant and nonionic surfactant are preferably used. Specific examples of the anionic surfactant include alkylacyl glutamate, alkyl phosphate, alkyl lactylate, dialkyl phosphate and trialkyl phosphate. Specific examples of nonionic surfactants include alkoxylated alkyl ethers, alkoxylated alkyl esters, alkyl polyglycosides, polyglyceryl esters and sugar esters. Most preferably, polysorbates belonging to nonionic surfactants are used. The amount of the surfactant is preferably 0.1 to 5.0% by weight based on the total weight of the nanoliposome.

The phospholipid is preferably a natural phospholipid or a synthetic phospholipid, but it is preferable to use a natural phospholipid lecithin. More preferably, the lecithin will be naturally derived unsaturated lecithin or saturated lecithin extracted from soybean or egg yolk. The lecithin is preferably 0.1 to 8.0% by weight based on the total weight of the nanoliposome.

The fatty acid is _{a saturated or unsaturated fatty acid of a C 12 -22} alkyl chain, such as lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid and linoleic acid. It will be preferable that the fatty acid is contained in an amount of 0.1 to 3.0% by weight based on the total weight of the nanoliposome.

It is preferable to use purified water as the water, and it will be preferable to be included in the remaining amount in constituting the nanoliposome.

The nanoliposome may be prepared through various methods, but most preferably, a mixture containing the above ingredients and a gombo cabbage extract are applied to a high pressure homogenizer. At this time, it is preferable to prepare nanoliposomes by passing through a high-pressure homogenizer 2 to 4 times under a pressure of 800 to 1000 bar.

The method for preparing the nanoliposome described above is only to describe an example of a known method for preparing a nanoliposome, and the nanoliposome of the present invention is not performed only by the method described above.

Claims of the present invention is a nano-liposome in which the extract of Gombo cabbage is collected, and all modified implementations for preparing it may be included in the scope of the present invention.

The nanoliposome prepared as above includes various ingredients included in cosmetic formulations, for example, stabilizers, solubilizers, vitamins, pigments, flavors or chelating agents, etc., and may be prepared in various formulations and supplied to the market. There will be.

Hereinafter, with reference to Examples, Comparative Examples and Experimental Examples, the anti-pollution cosmetic composition comprising the extract of Gombo cabbage according to the present invention will be described in detail.

Example One. Gombo cabbage Containing extract Anti-pollution Cosmetic Preparation of the composition

An anti-pollution cosmetic composition comprising the extract of Gombo cabbage of Example 1 was prepared through the following process.

Steps of preparing Gombo cabbage extract-The outpost of Gombo cabbage was mixed in 70% ethanol at a weight ratio of 1: 10, and extracted in a bath for 8 hours at 70°C to prepare a Gombo cabbage extract.

Nano-liposome preparation step-20% by weight of Gombo cabbage extract, 30% by weight of propylene glycol as polyol, 2.0% by weight of polysorbate as surfactant, 2.0% by weight of lecithin as phospholipid, 2.0% by weight of lauric acid as fatty acid, purified water in the balance After mixing, it was applied to a high pressure homogenizer to prepare a nanoliposome.

Dilution step-For the experiment, the nanoliposome and purified water were mixed in a weight ratio of 1:10.

Example 2 to 5. Different content Gombo cabbage Containing extract Anti-pollution Preparation of cosmetic composition

An anti-pollution cosmetic composition comprising the extract of Gombo cabbage with different contents of Examples 2 to 5 was prepared through the following process.

Steps of preparing Gombo cabbage extract-The outpost of Gombo cabbage was mixed in 70% ethanol at a weight ratio of 1: 10, and extracted in a bath for 8 hours at 70°C to prepare a Gombo cabbage extract.

Nanoliposome preparation step-each containing 0.1% by weight (Example 2), 10% by weight (Example 3), 30% by weight (Example 4), and 40% by weight (Example 5) of Gombo cabbage extract, and others After mixing 30% by weight of propylene glycol as a polyol, 2.0% by weight of polysorbate as a surfactant, 2.0% by weight of lecithin as a phospholipid, 2.0% by weight of lauric acid as a fatty acid, and purified water in the balance, applied to a high pressure homogenizer to nanoliposomes Was prepared.

Dilution step-For the experiment, the nanoliposome and purified water were mixed in a weight ratio of 1:10.

Comparative example One. Gombo cabbage Crushed filtrate Inclusive Cosmetic Preparation of the composition

For comparison of the experiment, a cosmetic composition comprising the filtrate of crushed gombo cabbage was prepared by simply pulverizing and filtering Gombo cabbage.

A cosmetic composition comprising the crushed filtrate of Gombo cabbage of Comparative Example 1 was prepared through the following manufacturing process.

Gombo cabbage crushing step-Gombo cabbage juice was prepared by crushing gombo cabbage.

Gombo Cabbage Filtration Step-Gombo Cabbage crushed filtrate was prepared by filtering the Gombo Cabbage juice generated from the crushed Gombo Cabbage with filter paper.

Dilution Step-For the experiment, the crushed filtrate of Gombo cabbage and purified water were mixed in a weight ratio of 1:10.

Comparative example 2. Gombo cabbage Containing extract Cosmetic Preparation of the composition

For comparison of the experiment, a cosmetic composition containing the extract of Gombo cabbage was prepared, which was not collected with nanoliposomes and only subjected to hot water extraction. A cosmetic composition including the extract of Gombo cabbage of Comparative Example 2 was prepared through the following manufacturing process.

Steps of preparing Gombo cabbage extract-The outpost of Gombo cabbage was mixed in 70% ethanol at a weight ratio of 1: 10, and extracted in a bath for 8 hours at 70°C to prepare a Gombo cabbage extract.

Dilution Step-For the experiment, the dilution of the gombo cabbage and purified water were mixed at a weight ratio of 1:10.

Experimental example 1. About benzopyrene Anti-pollution Measure the effect

Using skin keratinocytes that directly contact the pollutant, the degree of cell damage by the pollutant was measured, Examples 1 to 5 and Comparative Examples 1 to 2 were treated, and the effect of inhibiting skin cell damage was confirmed. Experiments were carried out according to known methods.

Human keratinocyte HaCaT ^{was dispensed into a 96-well plate at a concentration of 5 X 10 3} cells/ml and incubated for 24 hours, and then treated with benzopyrene (10 μM) and Examples 1 to 5 and Comparative Examples 1 to 2, and for 45 hours. It was further cultured. After that, 5mg/ml MTT solution was added and incubated for 3 hours, the medium was removed, 150ul of dimethyl sulfoxide was added to each well, shaken for 10 minutes, and the absorbance was measured at 570nm.

Anti-pollution effect on benzopyrene (10 μM) HaCaT cell proliferation rate (%) Example 1 67.6 Example 2 42.8 Example 3 63.1 Example 4 64.4 Example 5 34.2 Comparative Example 1 15.4 Comparative Example 2 28.3

As a result, according to the concentration, it was confirmed that the cell proliferation rate of HaCaT cells was changed, showing the highest cell proliferation rate in Example 1 in which the gombo cabbage extract was contained in 20% by weight, showing the highest anti-pollution effect for benzopyrene. appear. In addition, Example 2, Example 3, and Example 4 also showed satisfactory values, but Example 5 containing 40% by weight of Gombo cabbage extract exhibited a somewhat lower value.

In addition, in Comparative Example 1, which is a pulverized filtrate obtained by simply pulverizing and filtering Gombo cabbage, and Comparative Example 2 obtained by simple boiling water extraction, a somewhat lower value appears. It could be inferred that a bad effect occurred.

Experimental example 2. About ammonium nitrate Anti-pollution Measure the effect

Using skin keratinocytes that directly contact the pollutant, the degree of cell damage by the pollutant was measured, Examples 1 to 5 and Comparative Examples 1 to 2 were treated, and the effect of inhibiting skin cell damage was confirmed. Experiments were carried out according to known methods.

Human keratinocyte HaCaT ^{was dispensed into a 96-well plate at a concentration of 5 X 10 3} cells/ml and incubated for 24 hours, and then treated with ammonium nitrate (500 μg/ml) and Examples 1 to 5 and Comparative Examples 1 to 2. , Incubated for an additional 45 hours. After that, 5mg/ml MTT solution was added and incubated for 3 hours, the medium was removed, 150ul of dimethyl sulfoxide was added to each well, shaken for 10 minutes, and the absorbance was measured at 570nm.

Anti-pollution effect on ammonium nitrate (500㎍/ml) HaCaT cell proliferation rate (%) Example 1 62.9 Example 2 42.3 Example 3 58.4 Example 4 61.2 Example 5 31.5 Comparative Example 1 13.1 Comparative Example 2 24.7

As a result, according to the concentration, it could be confirmed that the cell proliferation rate of HaCaT cells was changed, showing the highest cell proliferation rate in Example 1 in which the Gombo cabbage extract was contained in 20% by weight, and the anti-pollution effect against ammonium nitrate was the highest. Appeared. In addition, Example 2, Example 3, and Example 4 also showed satisfactory values, but Example 5 containing 40% by weight of Gombo cabbage extract exhibited a somewhat lower value.

In addition, in Comparative Example 1, which is a pulverized filtrate obtained by simply pulverizing and filtering Gombo cabbage, and Comparative Example 2 obtained by simple boiling water extraction, a somewhat lower value appears. It could be inferred that a bad effect occurred.

Experimental example 3. To formaldehyde About Anti-pollution Measure the effect

Using skin keratinocytes that directly contact the pollutant, the degree of cell damage by the pollutant was measured, Examples 1 to 5 and Comparative Examples 1 to 2 were treated, and the effect of inhibiting skin cell damage was confirmed. Experiments were carried out according to known methods.

Human keratinocyte HaCaT ^{was dispensed into a 96-well plate at a concentration of 5 X 10 3} cells/ml and cultured for 24 hours, and then treated with formaldehyde (1.5 μg/ml) Examples 1 to 5 and Comparative Examples 1 to 2, Incubated for an additional 45 hours. After that, 5mg/ml MTT solution was added and incubated for 3 hours, the medium was removed, 150ul of dimethyl sulfoxide was added to each well, shaken for 10 minutes, and the absorbance was measured at 570nm.

Anti-pollution effect against formaldehyde (1.5㎍/ml) HaCaT cell proliferation rate (%) Example 1 58.2 Example 2 41.7 Example 3 55.4 Example 4 53.1 Example 5 27.0 Comparative Example 1 11.4 Comparative Example 2 23.2

As a result, according to the concentration, it could be confirmed that the cell proliferation rate of HaCaT cells was changed, and exhibited the highest cell proliferation rate in Example 1 in which the Gombo cabbage extract was contained in 20% by weight, showing the highest anti-pollution effect against formaldehyde. Appeared. In addition, Example 2, Example 3, and Example 4 also showed satisfactory values, but Example 5 containing 40% by weight of Gombo cabbage extract exhibited a somewhat lower value.

In addition, in Comparative Example 1, which is a pulverized filtrate obtained by simply pulverizing and filtering Gombo cabbage, and Comparative Example 2 obtained by simple boiling water extraction, a somewhat lower value appears. It could be inferred that a bad effect occurred.

conclusion.

The anti-pollution cosmetic composition comprising the extract of Gombo Cabbage of the present invention obtained by extracting Gombo Cabbage from Experimental Examples 1 to 3 and collecting it with nanoliposomes has an anti-pollution effect on benzopyrene, ammonium nitrate and formaldehyde in a concentration-dependent manner. It could be confirmed that it was shown.

In addition, nanoliposomes collected from Gombo cabbage extract into nanoliposomes have superior anti-pollution effects against benzopyrene, ammonium nitrate and formaldehyde compared to the pulverized filtrate obtained by simply pulverizing and filtering Gombo cabbage and the extract obtained by simple hot water extraction. It was confirmed that an unexpected effect occurred in the process of extracting Gombo cabbage and collecting it into nanoliposomes.

Therefore, the present invention is a novel substance having an anti-pollution effect, including the extract of Gombo cabbage extracted from Gombo cabbage, but the anti-pollution cosmetic comprising the extract of Gombo cabbage, characterized in that the extract of Gombo cabbage is collected in nanoliposomes. It is stated that the composition has been developed.

Although the present invention has been described with the accompanying drawings, this is only one of various embodiments including the gist of the present invention, and is intended to be easily implemented by those of ordinary skill in the art. As for the purpose, it is clear that the present invention is not limited to the above-described embodiments. Therefore, the scope of protection of the present invention should be interpreted by the following claims, and all technical ideas within the scope equivalent thereto by changes, substitutions, substitutions, etc. within the scope not departing from the gist of the present invention are subject to the rights of the present invention Will be included. In addition, it is clarified that some of the configurations in the drawings are provided in an exaggerated or reduced form than in actuality, as for describing the configuration more clearly.

Claims (5)

An anti-pollution cosmetic composition comprising a gombo cabbage extract extracted from gombo cabbage, wherein the gombo cabbage extract is collected in nanoliposomes.
The method of claim 1,
The cosmetic composition is an anti-pollution cosmetic composition comprising a gombo cabbage extract, characterized in that it has an anti-pollution effect.
The method of claim 1,
The gombo cabbage extract is an anti-pollution cosmetic composition comprising a gombo cabbage extract, characterized in that the jungtang extract of gombo cabbage.
The method of claim 1,
The nanoliposome is an anti-pollution cosmetic composition comprising a gombo cabbage extract, characterized in that the gombo cabbage extract is collected through a high pressure homogenizer.
The method of claim 1,
The gombo cabbage extract is an anti-pollution cosmetic composition comprising a gombo cabbage extract, characterized in that 0.1 to 30.0% by weight is included in the nanoliposome.

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