KR20210010599A - The Composition For Obesity-Preventing, Improving, and Curing Composed of Amomum villosum and Atractylodes macrocephala Extracts - Google Patents

Abstract

The present invention relates to a pharmaceutical composition and health functional food which are produced by mixing Amomum villosum and Atractylodes macrocephala extracts in a ratio of 1 : 1 to 1 : 3, and are effective in weight loss, and liver and epididymal weight reduction.

Description

The Composition For Obesity-Preventing, Improving, and Curing Composed of Amomum villosum and Atractylodes macrocephala Extracts containing Yangchunsa and Baekchul extract

The present invention relates to a composition for preventing, improving, and treating obesity containing Yangchunsa and Baekchul extract, and more particularly, to prevent obesity such as weight loss, weight reduction of adipose tissue of liver and epididymis , To a composition that is effective in improving and treating.

Due to excessive nutritional intake of modern people, fat is accumulated in the body due to metabolic disorders, and the number of patients suffering from obesity-associated diabetes and fatty liver disease is increasing.

The obese population is gradually increasing, and 1.5 billion of the world's adult population in 2008 are overweight and obese, and it is estimated that by 2030 the overweight population will reach about 2.1 billion.

In particular, as obesity formed in childhood, the size of adipocytes and the number of cells increase, which is more dangerous because the size of adipocytes decreases but the number of cells does not decrease during a lifetime when weight is decreased in adulthood.

In contrast, in adults, the adipocytes are enlarged but the number of cells does not increase. In addition, in the case of adults, the area and distribution of obesity tend to be confined to the abdomen. That is, only the size of fat cells in adults increases, but the number of fat cells increases with the size of fat cells in childhood obesity, so even if you lose weight in adulthood, only the size of fat cells temporarily decreases and the number of fat cells decreases. Because there are still many conditions, obesity can easily recur.

In order to prevent obesity, proper weight management is essential. Accordingly, as the necessity of weight management has recently emerged, various products are being released as dietary supplements, but side effects such as gastritis, constipation, and loss of appetite are caused.

The present applicant has no side effects by mixing the extract of Yangchunsa ( Amomum villosum ) and the extract of Baekchul ( Atractylodes macrocephala ), and has reached the completion of the present invention, which is effective against obesity without toxicity even after long-term administration.

Sine (砂仁) refers to a lump of ripe fruit or seeds of縮砂仁A momum xanthioides WALL. or Yangchunsa (陽春砂. Amomum villosum Lour.) belonging to the Zingiberaceae family. In the invention, the fruit of Yangchunsa was used as an ingredient.

Yangchunsa is mainly produced in Fujian, Guangdong, Guangxi, and Yunnan, and the congratulatory message is mainly produced in the Indochina Peninsula areas such as Cambodia, India, Laos, Myanmar, Thailand, Vietnam, and some areas of Guangxi and Yunnan in China (Flora). of China, v24:347-356.2000). The fruit of Hainan Temple (海南砂Amomum longiligulare TLWu), which is recognized as genuine in China, but treated as a dignity in Korea, is also being distributed in large quantities. Shen et al. (SHEN Li WANG Yang JIANG Ku et al. Comparison of HPLC Fingerprints of Amomum villosum Lour., Amomurn villosum Lour. var. xanthioides TLWu et Senjen and Amomum longiligulare TLWu. Chinese Pharmaceutical Journal. 2016: 51(12); 1039- 1043.) shows a high degree of similarity in the composition of the three types of deaths as a result of the RP-HPLC study. However, while the chemical composition of Haenamsa and Yangchunsa are similar, there is a clear difference from the previous two, so the pharmacological and clinical efficacy of this The comparison needs to be followed.

Baekchul ( Atractylodes macrocephala ) is a root mass of Sapju, a perennial herb belonging to the Asteraceae family, and its outer surface is gray or light brown. The cut surface is yellowish white, rich in fat, slightly fibrous, and has a characteristic aromatic odor. It is collected from October to November, and the fine roots, stems and leaves are removed and dried. Ingredients contain volatile essential oil, vitamin A and vitamin D. The nature is warm, slightly scented and non-poisonous, and the taste is a bit bitter and sweet. Efficacy is to dry the excess moisture that weighs on the body and allows urine to drain well. It also prevents sweating due to weakening of the skin and stabilizes the placenta and fetus.

Accordingly, the present invention uses a composition in which Yangchunsa and Baekchul extracts are mixed in a certain ratio, and shows the efficacy in inhibiting the size of adipocytes in the liver and suppressing the development of mRNA related to adipocyte differentiation in a high fat diet induced obesity model in vivo The invention was completed.

As a prior art for a pharmaceutical composition using an extract of Yangchunsa, Korean Patent No. 10-1793308 may be referred to, which is a pharmaceutical composition for the prevention or treatment of vomiting, comprising an extract of Amomum villosum as an active ingredient It is about. This is different from the anti-obesity effect, which is a pharmaceutical use pursued in the present invention.

Registered Patent 10-0543075, an inventor of a pharmaceutical composition using Baekchul extract, includes a method of extracting a component having an anti-allergic effect from Baekchul, a traditional herbal medicine, using hot water or an organic solvent, and an anti-allergic functional food containing the extracted substance or Disclosed is a pharmaceutical composition. However, this is also far from the pharmaceutical use of the present invention.

Korean Registered Patent Publication 10-1793308 Republic of Korea Patent Publication 10-0543075

The technical object of the present invention is to confirm the anti-obesity inhibitory effect of a mixture of Yangchunsa extract and Baekchul extract using a high fat diet induced obesity model, and provide a pharmaceutical composition corresponding thereto.

The technical object of the present invention is to provide a composition that is effective in the treatment and prevention of weight loss and obesity using a high fat diet induced obesity model in vivo .

In order to solve the above problems, the present invention provides a pharmaceutical composition for the prevention, improvement or treatment of obesity containing Yangchunsa and Baekchul extract as a means for solving the problems.

The Yangchunsa and Baekchul extracts of the present invention are mixed in a ratio of 1:1 to 1:3 by weight, and preferably contain 50 to 200mg/kg based on oral administration of mice.

Yangchunsa and Baekchul extract of the present invention can be used as a pharmaceutical composition for preventing, improving or treating obesity because it has an effect on weight loss, and can also be provided as a health functional food composition.

Since the extract of Yangchunsa and Baekchul of the present invention is effective in reducing weight, it is expected that it can be used as a corresponding pharmaceutical composition and health functional food.

1 shows the weight change of a high fat diet induced obese animal according to the dosage of a mixture of Yangchunsa and Baekchul extract.
Figure 2 shows the weight change of the liver weight (left view) and epididymis (right view) of a high fat diet induced obese animal according to the administration of a mixture of Yangchunsa and Baekchul extract.
3 shows the change in the amount of insulin in the blood according to the administration of a mixture of Yangchunsa and Baekchul extracts.
Figure 4 shows the change in the amount of triglycerides in blood (left) and liver (right) according to the administration of a mixture of Yangchunsa and Baekchul extracts.
5 shows the total cholesterol concentration (left) and LDL-cholesterol concentration (right) in the blood of a high fat diet induced obese animal according to the administration of a mixture of Yangchunsa and Baekchul extract.
6 is a change in phosphorylated Acetyl CoA Carboxylase (ACC) and fatty acid synthase (FAS) according to the administration of a mixture of Yangchunsa and Baekchul extracts (top), SREBP1 mRNA change (bottom left), and FAS mRNA change (bottom Right)

Hereinafter, the present invention will be described in more detail with reference to the drawings and examples. In relation to the present invention, the claims should in principle be interpreted as a case in which the technical content is described in the same manner as compared to the detailed description of the invention, that is, a case where matters described in the detailed description of the invention are described in the same claim. It should not be reduced and interpreted without special circumstances, and it should be understood to include all changes, equivalents, and substitutes included in the spirit and scope of the present invention.

The terms used in the present application are only used to describe specific embodiments, and are not intended to limit the present invention. Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention belongs.

Terms such as first, second, A, and B may be used to describe various elements, but the elements should not be limited by the terms, and the terms distinguish one element from other elements. It is used only for the purpose of doing. Also, the term "and/or" includes a combination of a plurality of related stated items or any of a plurality of related stated items.

Singular expressions include plural expressions, unless they clearly mean otherwise. In the present application, terms such as "comprise" or "have" are intended to designate the presence of elements, compounds, steps, numbers, temperatures, amounts, or combinations thereof described in the specification, but one or more other features. It is to be understood that the presence or addition of elements or components, compounds, steps, numbers, temperatures, amounts, or combinations thereof, does not preclude the possibility of addition.

Experimental example. Confirmation of anti-obesity efficacy of a mixture of Yangchunsa and Baekchul extracts using a high fat diet-induced obesity model

1) Preparation of Yangchunsa and Baekchul extract

Yangchunsa ( Amomum villosum ) and Baekchul ( Atractylodes macrocephala ), each 100g of which were confirmed to be authentic in the Department of Oriental Medicine, Wonkwang University, was heated under reflux extraction with 900ml of distilled water for 3 hours, filtered, and the filtrate was concentrated and freeze-dried under reduced pressure. g, to obtain 40.0 g of Baekchul extract.

A mixed extract obtained by mixing the above Yangchunsa extract and Baekchul extract in a ratio of 1:1 to 1:3 by weight was prepared, and all experiments were conducted on the same. E 1:1, E 1:2, E 1:3, or 1:1, 1:2, and 1:3 described in the examples and drawings represent the mixing ratio of the Yangchunsa extract and Baekchul extract extracted by the above method, respectively. it means.

In extracting Yangchunsa and Baekchul, different solvents may be used depending on conditions, and the extraction temperature, extraction time, amount of solvent, and residual component treatment method may be designed differently depending on the type of extraction solvent. Various solvents may be used as an extraction solvent, and extractable solvents include water, ethanol, methanol, fatty oil, glycerin, horse oil, ethyl acetate, acetone, butanol, and isopropanol.

2) Breeding and diet of experimental animals

As for the experimental animals, 6-week-old C57BL/6J male mice were purchased (Orientbio, Seongnam, Korea) and adapted to the environment of the breeding room for 1 week. Mice with an average weight of about 20 to 22 g were treated with a normal diet control group (Control), a high fat diet group, and a high fat diet with Yangchunsa and Baekchul extracts 1:1 mixture, 1:2 mixture, 1: 3 The mixture was divided into groups to which the mixture was administered, administered orally using a mouse zone, and then the experiment was conducted while observing changes in body weight. The total concentration of the orally administered Yangchunsa and Baekchul extract mixture was 100mg/kg.

The temperature of the breeding room was maintained at 18~24℃, and the relative humidity was 50~60%, and the contrast was adjusted in a 12-hour cycle (8:00~20:00). In the high fat feed used in this experiment, 15% of lard was added to the basic diet so that about 60% of the total calories were supplied as fat. Animal experiments were approved by the Wonkwang University Animal Experiment Ethics Committee, and the use and management of laboratory animals followed an institutional guideline.

3) Observation of weight change

The body weight of the experimental animals after 6 weeks of breeding and diet period was completed. As described above, the experimental group was divided into a control group, a high fat diet group, and a group administered with a 1:1 mixture of Yangchunsa and Baekchul extracts, a 1:2 mixture, and a 1:3 mixture on a high fat diet. Thus, the change in body weight was confirmed every week. The results are shown through FIG. 1. As shown in FIG. 1, the Yangchunsa and Baekchul extracts had an effect of reducing weight, and in particular, it was found that the higher the content of the Baekchul extract, the superior the effect of weight loss .

4) Weight measurement of liver and epididymis

The mouse was anesthetized with ether and then autopsied to extract the adipose tissue of the liver and epididymis, and the weight was measured, and the results are shown in FIG. 2. The left view of FIG. 2 shows the change in weight of the liver of the mouse, and the right view shows the change in the weight of the epididymis. As shown in Figure 2, the liver (liver) exhibited a weight change similar to that of the control group. This means that the mixture of extracts of Yangchunsa and Baekchul of the present invention has the effect of inhibiting the synthesis of triglycerides in the liver. Therefore, it can be seen that the mixture of extracts of Yangchunsa and Baekchul of the present invention has the effect of preventing and treating obesity such as the liver and epididymis.

The right figure shows the change in the weight of the epididymis. Although the effect of inhibiting the generation and growth of adipocytes was insignificant compared to the control group, it was confirmed that the effect of the high fat diet was remarkably superior to the administration group. Specifically, it was found that the higher the mixing ratio of Baekchul extract compared to Yangchunsa, the better the effect. Through this, it can be seen that mixing Baekchul extract is more effective in inhibiting the activation of the adipose tissue of the epididymis than simply using Yangchunsa extract.

5) Experiment to confirm changes in insulin in blood

To confirm the effect of inhibiting insulin secretion in the blood, as described above, the control group, the high fat diet group (High fat diet), the Yangchunsa and Baekchul extracts 1:1 mixture, 1:2 mixture, 1: Three groups were divided into groups to which the mixture was administered, and the concentration of the standard mixture concentrate was maintained at 100 mg/kg, and was bred for 6 weeks to investigate and analyze blood insulin. The results are shown through FIG. 3.

As shown in Figure 3, it was confirmed that the mixture of the extract of Yangchunsa and Baekchul of the present invention has the effect of inhibiting the increase in blood insulin caused by a high fat diet.

6) Triacylglycerol (TG) change test in blood and liver

In order to confirm the effect of inhibiting triglycerides in blood and liver, as described above, the control group (Control), the high fat diet group (High fat diet), the Yangchunsa and Baekchul extract 1:1 mixture, 1:2 mixture, 1 on a high fat diet. : The concentration of the standard mixture concentrate was kept at 100mg/kg and bred for 6 weeks, and the amount of triglycerides in blood and liver was investigated and analyzed. The results are shown through FIG. 4.

As shown in Figure 4, it was confirmed that the mixture of the extract of Yangchunsa and Baekchul of the present invention has the effect of inhibiting the increase in triglycerides of blood and liver due to a high fat diet.

7) Cholesterol inhibition test

To confirm the inhibitory effect of cholesterol, as described above, the control group, the high fat diet group, and the Yangchunsa and Baekchul extract 1:1 mixture, 1:2 mixture, and 1:3 mixture on a high fat diet Were divided into groups to which obesity was administered, and were bred for 6 weeks while maintaining the concentration of the standard mixture concentrate at 100 mg/kg to investigate and analyze total cholesterol and low-density cholesterol (LDL) in blood. The results are shown through FIG. 5.

As shown in FIG. 5, it was confirmed that the mixture of the extract of Yangchunsa and Baekchul of the present invention has the effect of inhibiting the increase of total cholesterol and low-density cholesterol (LDL) in blood due to a high fat diet.

8) Obesity induction-related genes expression inhibition test

In order to confirm the effect of suppressing the expression of genes related to obesity, as described above, as described above, the control group, the high fat diet group, the Yangchunsa and Baekchul extract 1:1 mixture, 1:2 mixture on a high fat diet, Each group was divided into groups to which the 1:3 mixture was administered and reared for 6 weeks while maintaining the concentration of the standard mixture concentrate at 100mg/kg while inducing obesity. After extracting the liver, intrahepatic SREBP1, p-ACC (phosphorylated ACC), The expression level of FAS was investigated and analyzed. The results are shown through FIG. 6.

As shown in Figure 6, it was confirmed that the mixture of the extract of Yangchunsa and Baekchul of the present invention has the effect of suppressing the expression of genes related to obesity induction by a high fat diet.

9) Statistical analysis

All experimental results were calculated as mean±standard deviation (Mean±SD). Statistical analysis according to the statistical significance test between each group was determined to be significant when p<0.05 by performing a one-way analysis of variance test (ANOVA) Duncan post-test comparison (SPSS V12., SPSS Inc, Chicago, IL, USA).

Manufacturing example. Composition for preventing or improving obesity containing extracts of Yangchunsa and Baekchul

As described above, the mixture of the extract of Yangchunsa and Baekchul of the present invention is effective in preventing and treating obesity after every time, and thus it can be formulated in a pharmaceutical unit dosage form by adding a pharmaceutically acceptable carrier, excipient, or diluent.

Here, the carrier, excipient, and diluent include tose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, undecided Vaginal cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, and the like can be used.

In addition, the pharmaceutical dosage form may be used in the form of a pharmaceutically acceptable salt, and may be used alone or in combination with other pharmaceutically active compounds as well as in an appropriate set.

In addition, when a composition containing the active ingredient is formulated, it may be prepared using a diluent or excipient such as a commonly used filler, extender, binder, wetting agent, disintegrant, or surfactant.

In addition, the pharmaceutical dosage forms are formulated in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, etc., external preparations, suppositories, and sterile injectable solutions according to a conventional method. Can be used.

In the solid formulation for oral administration, the composition may be prepared by mixing at least one excipient, for example, starch, calcium carbonate, sucrose, lactose, gelatin, and the like. Further, in addition to simple excipients, lubricants such as magnesium stearate and talc may also be used.

Formulations for parenteral administration may include sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized formulations, and suppositories.

As the non-aqueous solvent and suspension, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate may be used.

As a base for the suppository, witepsol, macrogol, tween 61, cacao butter, laurin paper, glycerogelatin, and the like may be used.

Meanwhile, the composition of the present invention can be used as a health functional food composition, for example, various foods, beverages, gum, tea, or vitamin complexes.

The health functional beverage composition of the present invention is not particularly limited to ingredients other than those containing the active ingredient, and may contain various flavoring agents or natural carbohydrates, as an additional ingredient, as in ordinary beverages.

Examples of the aforementioned natural carbohydrates include monosaccharides such as glucose, fructose; Disaccharides, such as maltose, sucrose, and the like, and polysaccharides, such as dextrin, cyclodextrin, and the like, and sugar alcohols such as xylitol, sorbitol, and erythritol. In addition to those described above, natural flavoring agents (taumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used as flavoring agents.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavors and natural flavoring agents, coloring agents and heavy weight agents (cheese, chocolate, etc.), pectic acid and its salts, organic acids, protection It may contain a colloidal thickener, a pH adjuster, a stabilizer, a preservative, a glycerin, an alcohol, a carbonate used in carbonated beverages, and the like.

Claims (1)

In the health functional food composition for preventing or improving obesity containing a mixture of Yangchunsa ( Amomum villosum ) extract and Baekchul ( Atractylodes macrocephala ) extract as an active ingredient,
The mixture,
Includes a total of 100mg/kg based on oral administration of mice,
Yangchunsa extract and Baekchul extract are mixed at a weight ratio of 1:3,
It has the effect of reducing weight loss, inhibiting the activation of adipose tissue in the epididymis, inhibiting the increase of blood insulin, inhibiting the increase of triglycerides in the blood and liver, inhibiting the increase of total cholesterol and low-density cholesterol (LDL) in the blood, and inhibiting the expression of obesity inducing genes. Health functional food composition for preventing or improving obesity.

Images