KR20200103230A - Method for the manufacture of probiotics, feed additive for herbivores with the main ingredient of [jangdae], a new kind of Kenaf - Google Patents

Abstract

The present invention relates to a method for producing probiotics, which are feed additives for herbivorous livestock such as beef cattle, cows, sheep, goats, and horses, more specifically, to a method for producing probiotics that are feed additives for herbivores with main ingredient of jangdae that is new kind of kenaf, in which bacillus subtilis, lactobacillul casei, lactobacillus plantrium, lactobacillus acidophilus and saccharomyces cerevisiae cultures are inoculated to jandae, which is a new kind of kenaf developed in Korea, and solid-fermented. Since new-kind jangdae is used as a main raw material for producing probiotics, which are feed additives, the protein content can be remarkably increased than corn, which is widely used as hay feed for beef cattle and cows, and microbial growth can be improved than mineral or sawdust used as an extender for general feed additives. Since the new-kind jangdae can be grown and used in Korea, it is possible to reduce the production cost of feed additives compared to the conventional kenaf imported from foreign countries.

Description

Method for the manufacture of probiotics, feed additive for herbivores with the main ingredient of [jangdae], a new kind of Kenaf}

The present invention relates to a method for producing a probiotic, which is a feed additive for herbivorous livestock such as beef cattle, dairy cows, sheep, goats, horses, etc., and Bacillus subtilis (Bacillus) in a new variety kenaf [jangdae] developed in Korea. subtilis), Lactobacillul casei, Lactobacillus plantrium, Lactobacillus acidophilus, yeast (Saccharomyces cerevisiae) cultures are inoculated for solid fermentation. It relates to a method for producing a probiotic, a feed additive for herbivorous livestock, using a new variety of kenaf (jangdae) as the main raw material.

Breeding livestock is constantly under stress from the breeding environment, and if homeostasis is broken due to this, it causes a change in the normal flora of the intestinal bacteria, which weakens the power of the beneficial bacteria colony, and when harmful bacteria proliferate, the feed absorption rate decreases due to the decline in intestinal function. . Therefore, to suppress the occurrence of various diseases caused by changes in the intestinal environment, prebiotics have been developed and used in humans, and are used as feed additives for livestock as probiotics. In addition, since the report that streptomycin added to feed improves the feed conversion ratio (FCR) of chicks, it has been widely used as an antibiotic. However, antibiotics remain in animal tissues and thus prevent pathogens harmful to humans or animals. As the use is regulated due to the risk of threatening human health by increasing antibiotic resistance, non-antibiotic physiologically active substances such as probiotics, enzymes, yeasts and growth hormones are widely used. Among them, probiotics are used as feed additives for the purpose of improving the intestinal environment of livestock or for the purpose of maintaining the health of livestock by promoting the growth of livestock and maintaining homeostasis by using the ability to produce special physiologically active substances. In addition, to maintain normal intestinal microbiological homeostasis, probiotics products using various types of microorganisms have been developed and used as feed additives.

The size of the world's direct benefit probiotics (DFM) market reached $784 million in 2016. The market grew at an annual average growth rate (CAGR) of 8.1% from 2017 to 2022, from $851 million in 2017 to about $1.3 billion in 2022, and in Korea, the market size of feed-added microbial products in 2016 was 1658 billion, with an annual average 15 It is expected to grow more than %. Moreover, the decrease in the production of soybean, wheat, and corn, which are feed crops due to climate change caused by global warming every year, is closely related to the productivity of breeding livestock, so it is recognized as an essential factor in improving the growth rate of livestock using probiotics.

Probiotics produced and used for feeding livestock are most important in maintaining the number of individuals capable of performing the functions of each constituent microorganism. Probiotics use a lot of minerals such as rice bran, barley bran and other minerals such as rice bran and barley bran, which can be easily and cheaply obtained in the vicinity as a moisture control agent or excipient in the production process, and other minerals such as loess, vermiculite, zeolite, illite, and sawdust that can be consumed by livestock. Probiotics are produced and sold by adding some of them. In general, excipients used in the manufacturing process of probiotics are mostly used for the function of microbial carriers, extenders, or moisture control. Since the early 1940s, kenaf has been used as forage, mainly in the United States, and kenaf has a high yield of up to 30 tons per ha. Kenaf is a mallow and annual herbaceous plant native to Africa and India, with a stem height of 3-5M. It contains up to 23% protein. In the case of fiber, it varies depending on the variety, but contains at least 23.9% and up to 29.1%. It has been used as a forage for herbivorous animals, including. In addition, various polyphenols such as flavonoids, which are antioxidants, and campphenols, which are functional physiologically active substances, and microbial probiotics such as vitamins and minerals, are fermented in the process of fermentation of calcium, phosphorus, potassium, magnesium, sodium, zinc, and iron. It contains useful substances and ingredients useful for breeding and livestock.

In this regard, to 100% by weight of kenaf, 0.01% by weight of Pediococcus pentosaceus, 0.01% by weight of Lactobacillus buchneri, 0.1% by weight of Lactobacillus plantarum and xylan The method of manufacturing kenaf silage, characterized by inoculating 0.1% by weight of degrading enzyme and fermenting at 0 to 40°C for 30 to 60 days, has been known as Korean Patent Publication No. 10-1898246, but carrier of probiotics, moisture Using Kenaf as a modulator and excipient, Bacillus subtilis, Lactobacillul casei, Lactobacillus plantarum, Lactobacillus acidophilus, yeast ( Saccharomyces cerevisiae) is a major difference from the present invention for solid culture.

Up to now, as in the present invention, kenaf, including a new variety [jangdae] useful for breeding livestock and microbial culture, is used as a microbial carrier, a moisture control agent, and as an excipient for a solid fermentation medium to maintain optimal and maximum viable bacteria count. There is no known method for preparing probiotics.

Korean Registered Patent Publication 10-0983791 (registered on September 16, 2010) Republic of Korea Patent Publication 10-2017-0051890 (published on May 12, 2017) Republic of Korea Patent Publication 10-0934252 (registered on December 18, 2009) Republic of Korea Patent Publication 10-1898246 (registered on September 6, 2018)

The present invention uses kenaf, an excipient different from the conventional method. Bacillus subtilis, Lactobacillul casei, Lactobacillus plantarum, Lactobacillus acidophilus, which help improve the growth rate and intestinal environment of livestock It is intended to provide a probiotic, a feed additive for herbivorous livestock, obtained by inoculating a yeast (Saccharomyces cerevisiae) culture into a kenaf [jangdae] used as a carrier in the present invention and fermenting it solid.

The present invention is used as a carrier, a moisture control agent, and an excipient for a microorganism to be cultured of kenaf powder, including a new variety [jangdae], in manufacturing a probiotic, a livestock feed additive. Microorganisms inoculated into kenaf are cultured with the addition of organic matter and minerals that satisfy each optimal growth characteristic. The amount of microbial culture added is inoculated and cultured at 0.01% to 0.5% by weight of the total weight.

Solid culture using kenaf as a carrier is carried out in a culture vessel in which temperature and humidity are controlled by mixing organic substances and inorganic substances. Bacillus subtilis, Lactobacillul casei, and Lactobacillus plantarium ( Lactobacillus plantarum), Lactobacillus acidophilus, and yeast (Saccharomyces cerevisiae) are cultured.

When culturing microbial solids using kenaf as a carrier, water is Bacillus subtilis, Lactobacillul casei, Lactobacillus plantarum, Lactobacillus acidophilus. ), etc., start culturing at 40-50%, and for yeast (Saccharomyces cerevisiae), start culturing in a solid medium containing 50-60% moisture.

By forcibly adjusting the moisture content of the kenaf carrier after solid culture is completed to less than 17%, unnecessary molds and various bacteria in the air are prevented from being contaminated.

Kenaf has a high content of protein and fiber, helps the growth of microorganisms such as calcium, potassium, magnesium, phosphorus, and iron, and contains a large amount of polyphenol, an antioxidant, so it is useful as a prebiotic that helps the growth of livestock. Have. It contains various trace elements, and its particle size is adjustable, so it can provide voids for microorganisms that require oxygen during solid culture. Unlike minerals, there is no heavy metal element that inhibits toxicity and bacterial growth, and vegetable fiber and tissue structure It is an ideal material that can be used as a carrier for attaching and growing microorganisms, and easy to control moisture.

Kenaf (jangdae) to be used in the present invention is a kenaf pulverized with a grinder from a minimum size of 1mm to 2mm to a maximum of 10mm to 30mm with a water content of 17% or less of the carrier on which the solid culture has been completed The pulverized material is mixed in a ratio of 5 to 10% by weight of a medium supplying protein, a carbon source medium supplying energy, and a medium supplying minerals and vitamins, to 90 to 95% by weight of the microbial culture medium.

Microorganisms used as probiotics, which are feed additives for livestock, grow in the tissues and pores of plants using kenaf as a tint.

The present invention uses a new kind of kenaf [jangdae] as the main raw material for manufacturing probiotics, which are feed additives, so that the protein content is much higher than that of corn, which is often used as hay feed for beef cattle or dairy cows, and an extender of general feed additives Microbial growth is better than minerals or sawdust used as a material, and since a new variety [pole] can be grown and used in Korea, it has the effect of reducing the production cost of feed additives compared to the conventional kenaf imported from foreign countries.

1 is a photograph of a real product of a feed additive, a probiotic, manufactured using a new variety of kenaf [jangdae] of the present invention as a main raw material

Hereinafter, the manufacturing process of the present invention will be described in detail by examples.

[Example]

(Manufacturing process 1)

In the case of Bacillus subtilis, which produces enzymes that degrade proteins and starch, 93% by weight of crushed kenaf [pole], 4% by weight of soy peptone as a medium, and 2% by weight of yeast extract %, 1% by weight of concentrated minerals from which only salt is removed from seawater in the clean sea area, water is added to 50% of moisture, mixed with a ribbon mixer, and incubated for 72 hours in a solid culture room with a culture temperature of 35℃ to 37℃ In the case of the control, ordinary sawdust was used instead of kenaf, and as a result of measuring the number of cultured individuals at 12 hour intervals, the average number of cells was 10 ⁷ ~ 10 ⁸ CFU (ColonyForming Unit) / gram.

※Example of three repeated experiments of 72 hours solid culture of Bacillus subtilis using Kenaf of Manufacturing Process 1 as an excipient

As a result of the above experiment, the number of bacteria in Bacillus subtilis was increased in the treatment group compared to the control group as 48 hours passed, and the difference was greater when cultured for 60 hours and 72 hours.

(Manufacturing process 2)

In the case of yeast (Saccharomyces cerevisiae), 3% by weight of corn extract powder, 2% by weight of yeast extract, and 1% by weight of concentrated mineral were mixed with 95% by weight of Kenaf [pole] pulverized product, and then water was added to 50% moisture. Then, the mixture was incubated for 72 hours in a solid culture chamber maintained at a culture temperature of 30°C to 36°C. In the case of the control, ordinary sawdust was used instead of kenaf. And as a result of measuring the number of viable cells at 12-hour intervals, the resulting number of cultured organisms averaged 10 ⁶ ~ 10 ⁷ CFU (ColonyForming Unit) / gram.

※Example of three repeated experiments of 72 hours solid culture of yeast (Saccharomyces cerevisiae) using Kenaf from Manufacturing Process 2 as an excipient

As a result of the above experiment, the number of cells of yeast (Saccharomyces cerevisiae) was increased the most in the treated group compared to the control group when cultured for 60 hours, and the width of the increase decreased when cultured for 72 hours.

(Manufacturing process 3)

Solid culture of Lactobacillul casei, Lactobacillus plantarum, and Lactobacillus acidophilus is a yeast extract 4 as a medium component in 90% by weight of the crushed material of Kenaf [pole] The medium was composed of weight%, soy peptone 5% by weight, and concentrated mineral 1% by weight, and water was mixed so that water was 50% by weight of the total weight, and the culture temperature was maintained at 30℃ ~ 37℃. Incubated for 120 hours indoors. When the pH fell below 5.0 by measuring the acidity (pH), the pH was maintained above 6.0 with this potassium phosphate (Dibasic). For the control group the cake was used instead of naphthyl general sawdust, and as a result the results of measuring the cell number of viable cells to be cultured object every 12 hours is an average ^{from 7} to 10 It was 10 ⁸ CFU (Colony Forming Unit)/gram.

※Example of 3 replicates of Lactobacillus spp. in solid culture for 120 hours using Kenaf from Manufacturing Process 3 as an excipient

As a result of the above experiment, the number of cells of Lactobacillus spp. began to increase in the treatment group compared to the control after 72 hours incubation, and the increase was the maximum at 84 hours incubation, and the increase in 96 and 108 hours incubation decreased, then 120 hours. It increased again during culture.

(Manufacturing process 4)

Each of the cells subjected to solid fermentation through the manufacturing processes 1, 2, and 3 are mixed with 30% by weight of bacillus in manufacturing process 1, 40% by weight of yeast in manufacturing process 2, and 30% by weight of lactobacillus in manufacturing process 3, and A probiotic, which is a feed additive for herbivorous livestock of the present invention, was prepared by forcibly controlling the moisture content of less than 17% to prevent contamination by unnecessary molds and various germs in the air.

The feed additive of the present invention prepared through the same process as described above is used as livestock feed by mixing 1 kg of the feed additive of the present invention with hay or 500 kg to 1,000 kg of thick feed, and the feed additive of the present invention is generally It is packaged and supplied in units of 20kg to farms and supplied in large quantities of 500kg to 1,000kg to feed factories. Kenaf used as the main raw material of the present invention is not only a new variety developed in Korea, but also general kenaf. Can also be used as.

Claims (3)

(One). In the case of Bacillus subtilis, which produces enzymes that degrade proteins and starch, 93% by weight of crushed kenaf [pole], 4% by weight of soy peptone as a medium, and 2% by weight of yeast extract %, 1% by weight of concentrated minerals from which only salt is removed from seawater in the clean sea area, water is added to 50% of moisture, mixed with a ribbon mixer, and incubated for 72 hours in a solid culture room with a culture temperature of 35℃ to 37℃ Steps to do,
(2). In the case of yeast (Saccharomyces cerevisiae), 3% by weight of corn extract powder, 2% by weight of yeast extract, and 1% by weight of concentrated mineral were mixed with 95% by weight of kenaf [pole] crushed product, and then water was added to 50% of moisture. And then incubating for 72 hours in a solid culture chamber maintained at a culture temperature of 30℃ to 36℃,
(3). Solid culture of Lactobacillul casei, Lactobacillus plantarum, and Lactobacillus acidophilus is a yeast extract 4 as a medium component in 90% by weight of the crushed material of Kenaf [pole] The medium was composed of weight%, soy peptone 5% by weight, and concentrated mineral 1% by weight, and water was mixed so that water was 50% by weight of the total weight, and the culture temperature was maintained at 30℃ ~ 37℃. Incubating for 120 hours at,
(4). Each of the cells obtained by solid fermentation through the steps (1), (2), and (3) was converted to 30% by weight of bacillus in step (1), 30% by weight of yeast in step (2), and lactobacillus in step (3). A herbivorous diet made from a new variety of kenaf [jangdae], characterized in that the probiotic, which is the feed additive of the present invention, is prepared by mixing each at 30% by weight and forcibly adjusting the moisture content to 17% or less. A method of manufacturing a probiotic, a feed additive for livestock. For herbivorous livestock, characterized by the use of kenaf, including a new variety of [jangdae], as a microbial carrier and excipient in the production of probiotics, which are feed additives for herbivorous livestock such as beef cattle, cows, sheep, goats, and horses. A method for producing a feed additive, a probiotic. The method of claim 1, wherein the kenaf [jangdae] is pulverized with a grinder so that the particle size is from 1mm to 2mm to the maximum size is 10mm to 30mm, and is used as a feed additive for herbivorous livestock. .

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